Journal articles: 'Tissues'

1

Bakhshandeh, Behnaz, Payam Zarrintaj, Mohammad Omid Oftadeh, Farid Keramati, Hamideh Fouladiha, Salma Sohrabi-jahromi, and Zarrintaj Ziraksaz. "Tissue engineering; strategies, tissues, and biomaterials." Biotechnology and Genetic Engineering Reviews 33, no. 2 (July 3, 2017): 144–72. http://dx.doi.org/10.1080/02648725.2018.1430464.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Somepalli, Gowthami, Sarthak Sahoo, Arashdeep Singh, and Sridhar Hannenhalli. "Prioritizing and characterizing functionally relevant genes across human tissues." PLOS Computational Biology 17, no. 7 (July 16, 2021): e1009194. http://dx.doi.org/10.1371/journal.pcbi.1009194.

Full text

Abstract:

Knowledge of genes that are critical to a tissue’s function remains difficult to ascertain and presents a major bottleneck toward a mechanistic understanding of genotype-phenotype links. Here, we present the first machine learning model–FUGUE–combining transcriptional and network features, to predict tissue-relevant genes across 30 human tissues. FUGUE achieves an average cross-validation auROC of 0.86 and auPRC of 0.50 (expected 0.09). In independent datasets, FUGUE accurately distinguishes tissue or cell type-specific genes, significantly outperforming the conventional metric based on tissue-specific expression alone. Comparison of tissue-relevant transcription factors across tissue recapitulate their developmental relationships. Interestingly, the tissue-relevant genes cluster on the genome within topologically associated domains and furthermore, are highly enriched for differentially expressed genes in the corresponding cancer type. We provide the prioritized gene lists in 30 human tissues and an open-source software to prioritize genes in a novel context given multi-sample transcriptomic data.

APA, Harvard, Vancouver, ISO, and other styles

3

Apa, Ludovica, Marianna Cosentino, Flavia Forconi, Antonio Musarò, Emanuele Rizzuto, and Zaccaria Del Prete. "The Development of an Innovative Embedded Sensor for the Optical Measurement of Ex-Vivo Engineered Muscle Tissue Contractility." Sensors 22, no. 18 (September 12, 2022): 6878. http://dx.doi.org/10.3390/s22186878.

Full text

Abstract:

Tissue engineering is a multidisciplinary approach focused on the development of innovative bioartificial substitutes for damaged organs and tissues. For skeletal muscle, the measurement of contractile capability represents a crucial aspect for tissue replacement, drug screening and personalized medicine. To date, the measurement of engineered muscle tissues is rather invasive and not continuous. In this context, we proposed an innovative sensor for the continuous monitoring of engineered-muscle-tissue contractility through an embedded technique. The sensor is based on the calibrated deflection of one of the engineered tissue’s supporting pins, whose movements are measured using a noninvasive optical method. The sensor was calibrated to return force values through the use of a step linear motor and a micro-force transducer. Experimental results showed that the embedded sensor did not alter the correct maturation of the engineered muscle tissue. Finally, as proof of concept, we demonstrated the ability of the sensor to capture alterations in the force contractility of the engineered muscle tissues subjected to serum deprivation.

APA, Harvard, Vancouver, ISO, and other styles

4

Tezcaner, A., G. Köse, and V. Hasırcı. "Fundamentals of tissue engineering: Tissues and applications." Technology and Health Care 10, no. 3-4 (July 8, 2002): 203–16. http://dx.doi.org/10.3233/thc-2002-103-406.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Patil, Amol Somaji, Yash Merchant, and Preethi Nagarajan. "Tissue Engineering of Craniofacial Tissues – A Review." journal of Regenerative Medicine and Tissue Engineering 2, no. 1 (2013): 6. http://dx.doi.org/10.7243/2050-1218-2-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Duance, Vic. "Connective tissue: Get connected with connective tissues." Biochemist 25, no. 5 (October 1, 2003): 7–10. http://dx.doi.org/10.1042/bio02505007.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Leong, Ivone. "New tissue processing technique for adipose tissues." Nature Reviews Endocrinology 14, no. 3 (January 29, 2018): 128. http://dx.doi.org/10.1038/nrendo.2018.8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Yoshizato, Katsutoshi. "Tissue reconstitution: metamorphosis, regeneration, and artificial tissues." Wound Repair and Regeneration 6, no. 4 (July 1998): 273–75. http://dx.doi.org/10.1046/j.1524-475x.1998.60403.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Villar, Cristina C., and David L. Cochran. "Regeneration of Periodontal Tissues: Guided Tissue Regeneration." Dental Clinics of North America 54, no. 1 (January 2010): 73–92. http://dx.doi.org/10.1016/j.cden.2009.08.011.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Rickles, Richard J., and Sidney Strickland. "Tissue plasminogen activator mRNA in murine tissues." FEBS Letters 229, no. 1 (February 29, 1988): 100–106. http://dx.doi.org/10.1016/0014-5793(88)80806-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Atala, Anthony. "Tissue engineering of reproductive tissues and organs." Fertility and Sterility 98, no. 1 (July 2012): 21–29. http://dx.doi.org/10.1016/j.fertnstert.2012.05.038.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

McCullen, Seth D., Andre GY Chow, and Molly M. Stevens. "In vivo tissue engineering of musculoskeletal tissues." Current Opinion in Biotechnology 22, no. 5 (October 2011): 715–20. http://dx.doi.org/10.1016/j.copbio.2011.05.001.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Golbad, Sara, and Mohammad Haghpanahi. "Hyperelastic Model Selection of Tissue Mimicking Phantom Undergoing Large Deformation and Finite Element Modeling for Elastic and Hyperelastic Material Properties." Advanced Materials Research 415-417 (December 2011): 2116–20. http://dx.doi.org/10.4028/www.scientific.net/amr.415-417.2116.

Full text

Abstract:

Pathologies in soft tissues are associated with changes in their elastic properties. Tumor tissues are usually stiffer than the fat tissues and other normal tissues and show the nonlinear behavior in large deformations. There have been a lot of researches about elastography (linear and nonlinear) as a new detecting technique based on mechanical behavior of tissue. In order to formulate the tissue’s nonlinear behavior, a strain energy function is required. For better estimation of nonlinear tissue parameters in elasticity imaging, non linear stress-strain curve of phantom is used. This work presents hyperelastic measurement results of tissue-mimicking phantom undergoing large deformation during uniaxial compression. For phantom samples, 8 hyperelastic models have been used. The results indicate that polynomial model with N=2 is the most accurate in terms of fitting experimental data. To compare the results between elastic and hyperelastic model, a 3-D finite element numerical model developed based on two different materials of elastic and hyperelastic material properties. The comparison confirm the approach of other recent studies about necessity of hyperelastic elastography and state that hyperelastic elastography should be used to formulate a technique for breast cancer diagnosis.

APA, Harvard, Vancouver, ISO, and other styles

14

Lahiri, Pooja, Suranjana Mukherjee, Biswajoy Ghosh, Debnath Das, Basudev Lahiri, Shailendra Kumar Varshney, Mousumi Pal, Ranjan Rashmi Paul, and Jyotirmoy Chatterjee. "Comprehensive Evaluation of PAXgene Fixation on Oral Cancer Tissues Using Routine Histology, Immunohistochemistry, and FTIR Microspectroscopy." Biomolecules 11, no. 6 (June 15, 2021): 889. http://dx.doi.org/10.3390/biom11060889.

Full text

Abstract:

The choice of tissue fixation is critical for preserving the morphology and biochemical information of tissues. Fragile oral tissues with lower tensile strength are challenging to process for histological applications as they are prone to processing damage, such as tissue tear, wrinkling, and tissue fall-off from slides. This leads to loss of morphological information and unnecessary delay in experimentation. In this study, we have characterized the new PAXgene tissue fixation system on oral buccal mucosal tissue of cancerous and normal pathology for routine histological and immunohistochemical applications. We aimed to minimize the processing damage of tissues and improve the quality of histological experiments. We also examined the preservation of biomolecules by PAXgene fixation using FTIR microspectroscopy. Our results demonstrate that the PAXgene-fixed tissues showed significantly less tissue fall-off from slides. Hematoxylin and Eosin staining showed comparable morphology between formalin-fixed and PAXgene-fixed tissues. Good quality and slightly superior immunostaining for cancer-associated proteins p53 and CK5/6 were observed in PAXgene-fixed tissues without antigen retrieval than formalin-fixed tissues. Further, FTIR measurements revealed superior preservation of glycogen, fatty acids, and amide III protein secondary structures in PAXgene-fixed tissues. Overall, we present the first comprehensive evaluation of the PAXgene tissue fixation system in oral tissues. This study concludes that the PAXgene tissue fixation system can be applied to oral tissues to perform diagnostic molecular pathology experiments without compromising the quality of the morphology or biochemistry of biomolecules.

APA, Harvard, Vancouver, ISO, and other styles

15

OKANO, TAKAHISA, SHINICHI SATOH, TAKAHIRO OKA, and TAKEHISA MATSUDA. "Tissue Engineering of Skeletal Muscle Highly Dense, Highly Oriented Hybrid Muscular Tissues Biomimicking Native Tissues." ASAIO Journal 43, no. 5 (September 1997): M753. http://dx.doi.org/10.1097/00002480-199709000-00084.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Aleinik, Aleksandr N., Natalya D. Turgunova, Victoria V. Velikaya, Ludmila I. Musabaeva, Zhanna A. Startseva, and Marat R. Mukhamedov. "Non-Invasive Tissue Injury Monitoring Using Bioimpedance Spectroscopy." Advanced Materials Research 1084 (January 2015): 413–16. http://dx.doi.org/10.4028/www.scientific.net/amr.1084.413.

Full text

Abstract:

An understanding of normal tissue response is necessary for the optimization of radiation treatment in cancer therapy. Cancer cells exhibit altered local dielectric properties compared to normal cells because of the difference in shape, size and orientation. These properties are measurable as a difference in electrical conductance using electrical impedance spectroscopy. Multiple frequency bioimpedance analysis is used to measure change in electrical properties of the irradiated tissues as a function of frequency and time. From the experimental results, it is clear that the electrical properties demonstrated good detection performance. The electrical parameters of the tissues could be used to distinguish the tissue's status. Changes in electrical properties at different frequencies show, that there are differences between conductivity of non-irradiated and irradiated tissues.

APA, Harvard, Vancouver, ISO, and other styles

17

Palmeri, Mark L., and Kathryn R. Nightingale. "Acoustic radiation force-based elasticity imaging methods." Interface Focus 1, no. 4 (June 8, 2011): 553–64. http://dx.doi.org/10.1098/rsfs.2011.0023.

Full text

Abstract:

Conventional diagnostic ultrasound images portray differences in the acoustic properties of soft tissues, whereas ultrasound-based elasticity images portray differences in the elastic properties of soft tissues (i.e. stiffness, viscosity). The benefit of elasticity imaging lies in the fact that many soft tissues can share similar ultrasonic echogenicities, but may have different mechanical properties that can be used to clearly visualize normal anatomy and delineate pathological lesions. Acoustic radiation force-based elasticity imaging methods use acoustic radiation force to transiently deform soft tissues, and the dynamic displacement response of those tissues is measured ultrasonically and is used to estimate the tissue's mechanical properties. Both qualitative images and quantitative elasticity metrics can be reconstructed from these measured data, providing complimentary information to both diagnose and longitudinally monitor disease progression. Recently, acoustic radiation force-based elasticity imaging techniques have moved from the laboratory to the clinical setting, where clinicians are beginning to characterize tissue stiffness as a diagnostic metric, and commercial implementations of radiation force-based ultrasonic elasticity imaging are beginning to appear on the commercial market. This article provides an overview of acoustic radiation force-based elasticity imaging, including a review of the relevant soft tissue material properties, a review of radiation force-based methods that have been proposed for elasticity imaging, and a discussion of current research and commercial realizations of radiation force based-elasticity imaging technologies.

APA, Harvard, Vancouver, ISO, and other styles

18

Hauser, Peter Viktor, Hsiao-Min Chang, Masaki Nishikawa, Hiroshi Kimura, Norimoto Yanagawa, and Morgan Hamon. "Bioprinting Scaffolds for Vascular Tissues and Tissue Vascularization." Bioengineering 8, no. 11 (November 6, 2021): 178. http://dx.doi.org/10.3390/bioengineering8110178.

Full text

Abstract:

In recent years, tissue engineering has achieved significant advancements towards the repair of damaged tissues. Until this day, the vascularization of engineered tissues remains a challenge to the development of large-scale artificial tissue. Recent breakthroughs in biomaterials and three-dimensional (3D) printing have made it possible to manipulate two or more biomaterials with complementary mechanical and/or biological properties to create hybrid scaffolds that imitate natural tissues. Hydrogels have become essential biomaterials due to their tissue-like physical properties and their ability to include living cells and/or biological molecules. Furthermore, 3D printing, such as dispensing-based bioprinting, has progressed to the point where it can now be utilized to construct hybrid scaffolds with intricate structures. Current bioprinting approaches are still challenged by the need for the necessary biomimetic nano-resolution in combination with bioactive spatiotemporal signals. Moreover, the intricacies of multi-material bioprinting and hydrogel synthesis also pose a challenge to the construction of hybrid scaffolds. This manuscript presents a brief review of scaffold bioprinting to create vascularized tissues, covering the key features of vascular systems, scaffold-based bioprinting methods, and the materials and cell sources used. We will also present examples and discuss current limitations and potential future directions of the technology.

APA, Harvard, Vancouver, ISO, and other styles

19

Kouadjo, Kouame E., Yuichiro Nishida, Jean F. Cadrin-Girard, Mayumi Yoshioka, and Jonny St-Amand. "Housekeeping and tissue-specific genes in mouse tissues." BMC Genomics 8, no. 1 (2007): 127. http://dx.doi.org/10.1186/1471-2164-8-127.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Caplan, Arnold I., and Victor M. Goldberg. "Principles of Tissue Engineered Regeneration of Skeletal Tissues." Clinical Orthopaedics and Related Research 367 (October 1999): S12—S16. http://dx.doi.org/10.1097/00003086-199910001-00003.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Martin, I., R. Quarto, B. Dozin, and R. Cancedda. "Producing prefabricated tissues and organs via tissue engineering." IEEE Engineering in Medicine and Biology Magazine 16, no. 2 (1997): 73–80. http://dx.doi.org/10.1109/51.582179.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Niederberger, Craig. "Re: Tissue Engineering of Reproductive Tissues and Organs." Journal of Urology 189, no. 3 (March 2013): 1038. http://dx.doi.org/10.1016/j.juro.2012.11.137.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Mardon, Helen J., and James T. Triffitt. "A tissue-specific protein in rat osteogenic tissues." Journal of Bone and Mineral Research 2, no. 3 (December 3, 2009): 191–99. http://dx.doi.org/10.1002/jbmr.5650020305.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Noda, Sawako, Yoshinori Sumita, Seigo Ohba, Hideyuki Yamamoto, and Izumi Asahina. "Soft tissue engineering with micronized-gingival connective tissues." Journal of Cellular Physiology 233, no. 1 (May 3, 2017): 249–58. http://dx.doi.org/10.1002/jcp.25871.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Kim, Suhon, Hanjun Hwangbo, SooJung Chae, and Hyeongjin Lee. "Biopolymers and Their Application in Bioprinting Processes for Dental Tissue Engineering." Pharmaceutics 15, no. 8 (August 10, 2023): 2118. http://dx.doi.org/10.3390/pharmaceutics15082118.

Full text

Abstract:

Dental tissues are composed of multiple tissues with complex organization, such as dentin, gingiva, periodontal ligament, and alveolar bone. These tissues have different mechanical and biological properties that are essential for their functions. Therefore, dental diseases and injuries pose significant challenges for restorative dentistry, as they require innovative strategies to regenerate damaged or missing dental tissues. Biomimetic bioconstructs that can effectively integrate with native tissues and restore their functionalities are desirable for dental tissue regeneration. However, fabricating such bioconstructs is challenging due to the diversity and complexity of dental tissues. This review provides a comprehensive overview of the recent developments in polymer-based tissue engineering and three-dimensional (3D) printing technologies for dental tissue regeneration. It also discusses the current state-of-the-art, focusing on key techniques, such as polymeric biomaterials and 3D printing with or without cells, used in tissue engineering for dental tissues. Moreover, the final section of this paper identifies the challenges and future directions of this promising research field.

APA, Harvard, Vancouver, ISO, and other styles

26

Tajikawa, Tsutomu, Yota Sekido, Kazuki Mori, Takayuki Kawashima, Yumiko Nakashima, Shinji Miyamoto, and Yasuhide Nakayama. "Diverse Shape Design and Physical Property Evaluation of In-Body Tissue Architecture-Induced Tissues." Bioengineering 11, no. 6 (June 12, 2024): 598. http://dx.doi.org/10.3390/bioengineering11060598.

Full text

Abstract:

Autologous-engineered artificial tissues constitute an ideal alternative for radical surgery in terms of natural anticoagulation, self-repair, tissue regeneration, and the possibility of growth. Previously, we focused on the development and practical application of artificial tissues using “in-body tissue architecture (iBTA)”, a technique that uses living bodies as bioreactors. This study aimed to further develop iBTA by fabricating tissues with diverse shapes and evaluating their physical properties. Although the breaking strength increased with tissue thickness, the nominal breaking stress increased with thinner tissues. By carving narrow grooves on the outer periphery of an inner core with narrow grooves, we fabricated approximately 2.2 m long cord-shaped tissues and net-shaped tissues with various designs. By assembling the two inner cores inside the branched stainless-steel pipes, a large graft with branching was successfully fabricated, and its aortic arch replacement was conducted in a donor goat without causing damage. In conclusion, by applying iBTA technology, we have made it possible, for the first time, to create tissues of various shapes and designs that are difficult using existing tissue-engineering techniques. Thicker iBTA-induced tissues exhibited higher rupture strength; however, rupture stress was inversely proportional to thickness. These findings broaden the range of iBTA-induced tissue applications.

APA, Harvard, Vancouver, ISO, and other styles

27

Brett Kahr. "Tissues." American Imago 65, no. 2 (2008): 299–308. http://dx.doi.org/10.1353/aim.0.0013.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Shi, Chun-Sheng, Na Shu, Li-Li Jiang, and Bo Jiang. "Expression and role of specificity protein 1 and collagen I in recurrent pterygial tissues." International Journal of Ophthalmology 14, no. 2 (February 18, 2021): 223–27. http://dx.doi.org/10.18240/ijo.2021.02.07.

Full text

Abstract:

AIM: To investigate the expression profiles of the transcription factor specificity protein 1 (Sp1) and collagen I in recurrent pterygial tissues. What is more, to compare the changes of Sp1 and collagen I among primary pterygial tissue, recurrent pterygial tissue and conjunctival tissue. METHODS: In the prospective study, we collected the pterygial tissues of 40 patients who underwent resection of primary pterygial tissue and recurrent pterygial tissue, and the conjunctival tissues of 10 patients with enucleation due to trauma. The relative expression levels of Sp1 and collagen I were analyzed by reverse transcription quantitative-polymerase chain reaction and Western blot. Paired t-test was performed to compare the Sp1 and collagen I of recurrent pterygial tissues, as well as the primary pterygial tissues and conjunctival tissues. In further, Pearson’s hypothesis testing of correlation coefficients was used to compare the correlations of Sp1 and Collagen I. RESULTS: The content of Sp1 and collagen I mRNA and protein was significantly greater in recurrent pterygial tissue than that was in primary and conjunctival tissue (P<0.05). There was a positive correlation between the mRNA and protein levels of Sp1 and collagen I in recurrent pterygial tissues (protein: r=0.913, P<0.05; mRNA: r=0.945, P<0.05). CONCLUSION: Sp1 and collagen I are expressed in normal conjunctival, primary, and recurrent pterygial tissues, but expression is significantly greater in the latter. Sp1 and collagen I may be involved in the regulation of the development of recurrent pterygium.

APA, Harvard, Vancouver, ISO, and other styles

29

Cheema, Umber. "Position Paper Progress in the development of biomimetic engineered human tissues." Journal of Tissue Engineering 14 (January 2023): 204173142211456. http://dx.doi.org/10.1177/20417314221145663.

Full text

Abstract:

Tissue engineering (TE) is the multi-disciplinary approach to building 3D human tissue equivalents in the laboratory. The advancement of medical sciences and allied scientific disciplines have aspired to engineer human tissues for three decades. To date there is limited use of TE tissues/organs as replacement body parts in humans. This position paper outlines advances in engineering of specific tissues and organs with tissue-specific challenges. This paper outlines the technologies most successful for engineering tissues and key areas of advancement.

APA, Harvard, Vancouver, ISO, and other styles

30

Zamay, G. S., I. V. Belayanina, A. S. Zamay, M. A. Komarova, A. V. Krat, E. N. Eremina, R. A. Zukov, A. E. Sokolov, and T. N. Zamay. "DNA aptamers selection for breast cancer." Biomeditsinskaya Khimiya 62, no. 4 (2016): 411–17. http://dx.doi.org/10.18097/pbmc20166204411.

Full text

Abstract:

A method of selection of DNA aptamers to breast tumor tissue based on the use of postoperative material has been developed. Breast cancer tissues were used as the positive target; the negative targets included benign tumor tissue, adjacent healthy tissues, breast tissues from mastopathy patients, and also tissues of other types of malignant tumors. During selection a pool of DNA aptamers demonstrating selective binding to breast cancer cells and tissues and insignificant binding to breast benign tissues has been obtained. These DNA aptamers can be used for identification of protein markers, breast cancer diagnostics, and targeted delivery of anticancer drugs.

APA, Harvard, Vancouver, ISO, and other styles

31

Ferris, Jennifer S., Tian Wang, Shuang Wang, Hanina Hibshoosh, Tao Su, Xiaomei Wang, Xiaowei Chen, et al. "Identifying DNA methylation signatures in high-grade serous ovarian cancer: Results vary by control tissue type." Journal of Clinical Oncology 40, no. 16_suppl (June 1, 2022): e17559-e17559. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.e17559.

Full text

Abstract:

e17559 Background: High grade serous ovarian cancer (HGSC) is the most common and fatal epithelial ovarian cancer and is often diagnosed in late stages. DNA methylation has emerged as a potential biomarker for the early detection of cancer, including ovarian cancer. Studies examining DNA methylation in ovarian tumor tissue have used adjacent non-tumor tissues or tissues from unaffected women as the control; however, few have used paired tissue and research is sparse on how results may vary by the control non-tumor tissue type. Therefore, we examined DNA methylation signatures in HGSC tumor tissue using different control non-tumor tissue types. Methods: We examined DNA methylation signatures using the Illumina Infinium MethylationEPIC BeadChip (850k) in formalin-fixed paraffin-embedded tissue. In women with HGSC, we compared DNA methylation patterns between paired adjacent non-tumor ovary (n = 74) and fallopian tube (n = 80) tissues. We compared DNA methylation patterns in these adjacent non-tumor tissues with non-tumor ovary (n = 8) and fallopian tube (n = 8) tissues from unaffected women without ovarian cancer carrying a pathogenic variant in BRCA1/2 ( BRCA1/2+). Lastly, we compared the overlap in differentially methylated CpGs identified in HGSC tumor tissue compared to paired adjacent non-tumor ovary (n = 50) and fallopian tube (n = 52) tissues. We processed the methylation data and used principal components analysis (PCA) and the adjusted Rand Index to compare the non-tumor tissue type methylation patterns. We used a paired t-test between individual-matched tumor and adjacent non-tumor tissue for each CpG site and then applied Bonferroni’s method to adjust the obtained p-values. Results: PCA comparing methylation patterns between paired adjacent non-tumor ovary and fallopian tube tissues from women with HGSC showed an adjusted Rand Index of 0.78, revealing separate clusters that cannot be combined. PCA comparing methylation patterns from the adjacent non-tumor tissues from women with HGSC and the non-tumor tissues from unaffected BRCA1/2+ women showed an adjusted Rand Index of -0.10 and 0.07 for the ovary and fallopian tube tissues, respectively, revealing overlapping clusters that can be combined. Lastly, comparison of the top differentially methylated CpGs identified in HGSC tumor tissue when using paired adjacent non-tumor ovary versus fallopian tube tissues as the control showed minimal overlap (6.8% and 4.0% for hypermethylated and hypomethylated CpGs, respectively). Conclusions: These results suggest that paired adjacent non-tumor ovary and fallopian tube tissues from women with HGSC have different DNA methylation patterns and result in different methylation signatures identified in HGSC tumor tissue. When comparing results across studies, including for validation, the type of non-tumor tissue control must be considered.

APA, Harvard, Vancouver, ISO, and other styles

32

Ferris, Jennifer S., Tian Wang, Shuang Wang, Hanina Hibshoosh, Tao Su, Xiaomei Wang, Xiaowei Chen, et al. "Identifying DNA methylation signatures in high-grade serous ovarian cancer: Results vary by control tissue type." Journal of Clinical Oncology 40, no. 16_suppl (June 1, 2022): e17559-e17559. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.e17559.

Full text

Abstract:

e17559 Background: High grade serous ovarian cancer (HGSC) is the most common and fatal epithelial ovarian cancer and is often diagnosed in late stages. DNA methylation has emerged as a potential biomarker for the early detection of cancer, including ovarian cancer. Studies examining DNA methylation in ovarian tumor tissue have used adjacent non-tumor tissues or tissues from unaffected women as the control; however, few have used paired tissue and research is sparse on how results may vary by the control non-tumor tissue type. Therefore, we examined DNA methylation signatures in HGSC tumor tissue using different control non-tumor tissue types. Methods: We examined DNA methylation signatures using the Illumina Infinium MethylationEPIC BeadChip (850k) in formalin-fixed paraffin-embedded tissue. In women with HGSC, we compared DNA methylation patterns between paired adjacent non-tumor ovary (n = 74) and fallopian tube (n = 80) tissues. We compared DNA methylation patterns in these adjacent non-tumor tissues with non-tumor ovary (n = 8) and fallopian tube (n = 8) tissues from unaffected women without ovarian cancer carrying a pathogenic variant in BRCA1/2 ( BRCA1/2+). Lastly, we compared the overlap in differentially methylated CpGs identified in HGSC tumor tissue compared to paired adjacent non-tumor ovary (n = 50) and fallopian tube (n = 52) tissues. We processed the methylation data and used principal components analysis (PCA) and the adjusted Rand Index to compare the non-tumor tissue type methylation patterns. We used a paired t-test between individual-matched tumor and adjacent non-tumor tissue for each CpG site and then applied Bonferroni’s method to adjust the obtained p-values. Results: PCA comparing methylation patterns between paired adjacent non-tumor ovary and fallopian tube tissues from women with HGSC showed an adjusted Rand Index of 0.78, revealing separate clusters that cannot be combined. PCA comparing methylation patterns from the adjacent non-tumor tissues from women with HGSC and the non-tumor tissues from unaffected BRCA1/2+ women showed an adjusted Rand Index of -0.10 and 0.07 for the ovary and fallopian tube tissues, respectively, revealing overlapping clusters that can be combined. Lastly, comparison of the top differentially methylated CpGs identified in HGSC tumor tissue when using paired adjacent non-tumor ovary versus fallopian tube tissues as the control showed minimal overlap (6.8% and 4.0% for hypermethylated and hypomethylated CpGs, respectively). Conclusions: These results suggest that paired adjacent non-tumor ovary and fallopian tube tissues from women with HGSC have different DNA methylation patterns and result in different methylation signatures identified in HGSC tumor tissue. When comparing results across studies, including for validation, the type of non-tumor tissue control must be considered.

APA, Harvard, Vancouver, ISO, and other styles

33

Schmidt, Christine E., and Jennie M. Baier. "Acellular vascular tissues: natural biomaterials for tissue repair and tissue engineering." Biomaterials 21, no. 22 (November 2000): 2215–31. http://dx.doi.org/10.1016/s0142-9612(00)00148-4.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Okano, Takahisa, and Takehisa Matsuda. "Muscular Tissue Engineering: Capillary-Incorporated Hybrid Muscular Tissues in Vivo Tissue Culture." Cell Transplantation 7, no. 5 (September 1998): 435–42. http://dx.doi.org/10.1177/096368979800700502.

Full text

Abstract:

Requirements for a functional hybrid muscular tissue are 1) a high density of multinucleated cells, 2) a high degree of cellular orientation, and 3) the presence of a capillary network in the hybrid tissue. Rod-shaped hybrid muscular tissues composed of C2C12 cells (skeletal muscle myoblast cell line) and type I collagen, which were prepared using the centrifugal cell-packing method reported in our previous article, were implanted into nude mice. The grafts, comprised three hybrid tissues (each dimension, diameter, approximately 0.3 mm, length, approximately 1 mm, respectively), were inserted into the subcutaneous spaces on the backs of nude mice. All nude mice that survived the implantation were sacrificed at 1, 2, and 4 wk after the implantation. The grafts were easily distinguishable from the subcutaneous tissues of host mice with implantation time. The grafts increased in size with time after implantation, and capillary networks were formed in the vicinities and on the surfaces of the grafts. One week after implantation, many capillaries formed in the vicinities of the grafts. In the central portion of the graft, few capillaries and necrotic cells were observed. Mononucleated myoblasts were densely distributed and a low number of multinucleated myotubes were scattered. Two weeks after implantation, the formation of a capillary network was induced, resulting in the surfaces of the grafts being covered by capillaries. Numerous elongated multinucleated myotubes and mononucleated myoblasts were densely distributed and numerous capillaries were observed throughout the grafts. Four weeks after implantation a dense capillary network was formed in the vicinities and on the surfaces of the grafts. In the peripheral portion of the graft, multinucleated myotubes in the vicinities of the rich capillaries were observed. Thus, hybrid muscular tissues in vitro preconstructed was remodeled in vivo, which resulted in facilitating the incorporation of capillary networks into the tissues. © 1998 Elsevier Science Inc.

APA, Harvard, Vancouver, ISO, and other styles

35

Xu, Wenjian, Xuanshi Liu, Fei Leng, and Wei Li. "Blood-based multi-tissue gene expression inference with Bayesian ridge regression." Bioinformatics 36, no. 12 (April 11, 2020): 3788–94. http://dx.doi.org/10.1093/bioinformatics/btaa239.

Full text

Abstract:

Abstract Motivation Gene expression profiling is widely used in basic and cancer research but still not feasible in many clinical applications because tissues, such as brain samples, are difficult and not ethnical to collect. Gene expression in uncollected tissues can be computationally inferred using genotype and expression quantitative trait loci. No methods can infer unmeasured gene expression of multiple tissues with single tissue gene expression profile as input. Results Here, we present a Bayesian ridge regression-based method (B-GEX) to infer gene expression profiles of multiple tissues from blood gene expression profile. For each gene in a tissue, a low-dimensional feature vector was extracted from whole blood gene expression profile by feature selection. We used GTEx RNAseq data of 16 tissues to train inference models to capture the cross-tissue expression correlations between each target gene in a tissue and its preselected feature genes in peripheral blood. We compared B-GEX with least square regression, LASSO regression and ridge regression. B-GEX outperforms the other three models in most tissues in terms of mean absolute error, Pearson correlation coefficient and root-mean-squared error. Moreover, B-GEX infers expression level of tissue-specific genes as well as those of non-tissue-specific genes in all tissues. Unlike previous methods, which require genomic features or gene expression profiles of multiple tissues, our model only requires whole blood expression profile as input. B-GEX helps gain insights into gene expressions of uncollected tissues from more accessible data of blood. Availability and implementation B-GEX is available at https://github.com/xuwenjian85/B-GEX. Supplementary information Supplementary data are available at Bioinformatics online.

APA, Harvard, Vancouver, ISO, and other styles

36

Rosati, Adolfo, Silvia Caporali, Sofiene B. M. Hammami, Inmaculada Moreno-Alías, Andrea Paoletti, and Hava F. Rapoport. "Tissue size and cell number in the olive (Olea europaea) ovary determine tissue growth and partitioning in the fruit." Functional Plant Biology 39, no. 7 (2012): 580. http://dx.doi.org/10.1071/fp12114.

Full text

Abstract:

The relationship between tissue size and cell number in the ovary and tissue size in the fruit, was studied in eight olive (Olea europaea L.) cultivars with different fruit and ovary size. All tissues in the ovary increased in size with increasing ovary size. Tissue size in the fruits correlated with tissue size in the ovary for both mesocarp and endocarp, but with different correlations: the mesocarp grew about twice as much per unit of initial volume in the ovary. Tissue size in the fruit also correlated with tissue cell number in the ovary. In this case, a single regression fitted all data pooled for both endocarp and mesocarp, implying that a similar tissue mass was obtained in the fruit per initial cell in the ovary, independent of tissues and cultivars. Tissue relative growth from bloom to harvest (i.e. the ratio between final and initial tissue size) differed among cultivars and tissues, but correlated with tissue cell size at bloom, across cultivars and tissues. These results suggest that in olive, tissue growth and partitioning in the fruit is largely determined by the characteristics of the ovary tissues at bloom, providing important information for plant breeding and crop management.

APA, Harvard, Vancouver, ISO, and other styles

37

Egelston, Colt, Weihua Guo, Eliza Bacon, Kena Ihle, Diana L. Simons, Christian Avalos, Jiayi Tan, et al. "Organ specificity dictates tumor immune infiltration and composition in metastatic breast cancer; lessons from a rapid autopsy tissue collection study." Journal of Clinical Oncology 38, no. 15_suppl (May 20, 2020): 1032. http://dx.doi.org/10.1200/jco.2020.38.15_suppl.1032.

Full text

Abstract:

1032 Background: Immune composition in the tumor microenvironment (TME) of patient tumors has proven to play a central role in the propensity of tumors to metastasize and respond to therapy. Evidence has suggested that the metastatic TME is immune aberrant, however limited sample size and numbers has made assessment of the immune TME in the development of multi-organ metastases difficult. Here we utilize a rapid autopsy tissue collection protocol to assess the infiltration and composition of the immune TME in numerous metastatic tissue sites, paired disease-free tissue sites, and the associated tissue draining lymph nodes. Methods: Post-mortem tissues were collected from six metastatic breast cancer patients shortly after death through City of Hope’s “Legacy Project for Rapid Tissue Donation” Program. The average post mortem interval (PMI) for tissue collection was 6 hours. Collected specimens include metastatic lesions and paired non-cancer samples from every cancer-involved organ, disease-free specimens from non-involved major organs, distant and tumor-draining lymph nodes (both cancer-infiltrated and disease free), as well as blood. Immediately following collection, specimens were processed into single cell suspension for flow cytometry. Over 80 immune cell phenotypes were assessed, including CD8+ and CD4+ T cell subsets, B cell subsets, natural killer (NK) cells, tumor associated macrophages (TAMs), dendritic cell subsets, and other cells. Results: Tumor infiltrated tissues were found to have comparable immune cell densities and composition compared to paired disease-free tissues of the same organ type. However, immune cell densities in metastatic tissues and disease-free tissues were significantly different between organ types, with lung immune infiltration consistently being greater than liver tissues. Differences in immune composition between tissue sites were also observed. Notably, liver tissues favored the presence of central memory CD8+ T cells, while lung tissues favored the presence of CD8+ tissue resident memory T cells. Relative to disease-free lung tissues, tumor infiltrated lungs contained diminished frequencies of CD8+ tissue resident memory T cells and altered B cell phenotypes. Conclusions: These data suggest that immune monitoring and trafficking of metastatic tissues site is dictated by organ type, which can be altered in composition by tumor infiltration. Further studies such as these may reveal organ-specific mechanisms of response to therapeutic interventions.

APA, Harvard, Vancouver, ISO, and other styles

38

Azril, Kuo-Yuan Huang, Jonathan Hobley, Mehdi Rouhani, Wen-Lung Liu, and Yeau-Ren Jeng. "A methodology to evaluate different histological preparations of soft tissues: Intervertebral disc tissues study." Journal of Applied Biomaterials & Functional Materials 21 (January 2023): 228080002311556. http://dx.doi.org/10.1177/22808000231155634.

Full text

Abstract:

A tissue preparation method will inevitably alter the tissue content. This study aims to evaluate how different common sample preparation methods will affect the tissue morphology, biomechanical properties, and chemical composition of samples. The study focuses on intervertebral disc (IVD) tissue; however, it can be applied to other soft tissues. Raman spectroscopy synchronized with nanoindentation instrumentation was employed to investigate the compositional changes of IVD, specifically, nucleus pulposus (NP) and annulus fibrosus (AF), together with their biomechanical properties of IVD. These properties were examined through the following histological specimen types: fresh cryosection (control), fixed cryosection, and paraffin-embedded. The IVD tissue could be located using an optical microscope under three different preparation methods. Paraffin-embedded samples showed the most explicit details where the lamellae structure of AF could be identified. In terms of biomechanical properties, there was no significant difference between the fresh and fixed cryosection ( p > 0.05). In contrast, the fresh cryosection and paraffin-embedded samples showed a significant difference ( p < 0.05). It was also found that the tissue preparations affected the chemical content of the tissues and structure of the tissue, which are expected to contribute to biomechanical properties changes. Fresh cryosection and fixed cryosection samples are more promising to work with for biomechanical assessment in histological tissues. The findings fill essential gaps in the literature by providing valuable insight into the characteristics of IVD at the microscale. This study can also become a reference for a better approach to assessing the mechanical properties and chemical content of soft tissues at the microscale.

APA, Harvard, Vancouver, ISO, and other styles

39

Haraguchi, Yuji, Akiyuki Hasegawa, Katsuhisa Matsuura, Mari Kobayashi, Shin-ichi Iwana, Yasuhiro Kabetani, and Tatsuya Shimizu. "Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of Its Synchronous Beatings by Optical Coherence Tomography." BioMed Research International 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/5341702.

Full text

Abstract:

Three-dimensional (3D) tissues are engineered by stacking cell sheets, and these tissues have been applied in clinical regenerative therapies. The optimal fabrication technique of 3D human tissues and the real-time observation system for these tissues are important in tissue engineering, regenerative medicine, cardiac physiology, and the safety testing of candidate chemicals. In this study, for aiming the clinical application, 3D human cardiac tissues were rapidly fabricated by human induced pluripotent stem (iPS) cell-derived cardiac cell sheets with centrifugation, and the structures and beatings in the cardiac tissues were observed cross-sectionally and noninvasively by two optical coherence tomography (OCT) systems. The fabrication time was reduced to approximately one-quarter by centrifugation. The cross-sectional observation showed that multilayered cardiac cell sheets adhered tightly just after centrifugation. Additionally, the cross-sectional transmissions of beatings within multilayered human cardiac tissues were clearly detected by OCT. The observation showed the synchronous beatings of the thicker 3D human cardiac tissues, which were fabricated rapidly by cell sheet technology and centrifugation. The rapid tissue-fabrication technique and OCT technology will show a powerful potential in cardiac tissue engineering, regenerative medicine, and drug discovery research.

APA, Harvard, Vancouver, ISO, and other styles

40

López-Ribot, Jose Luis, Maria Novella Vespa, and W. LaJean Chaffin. "Adherence of Candida albicans germ tubes to murine tissues in an ex vivo assay." Canadian Journal of Microbiology 40, no. 1 (January 1, 1994): 77–81. http://dx.doi.org/10.1139/m94-013.

Full text

Abstract:

Adhesion of Candida albicans germ tubes to murine tissues was examined. An ex vivo assay previously employed to examine adhesion of yeast cells of C. albicans was adapted for use with germ tubes. Binding of germ tubes to kidney, liver, spleen, and lymph node tissues was found to occur throughout the tissue section, with little tissue morphologic specificity. In general, more organisms adhered to spleen and lymph node tissues than to kidney and liver tissues. Observation of adhesion with scanning electron microscopy showed three germ tube – tissue interactions described as loose, tight, or embedded.Key words: Candida, germ tubes, adhesion, ex vivo.

APA, Harvard, Vancouver, ISO, and other styles

41

Chen, Xiaoyu, Hyunwoo Yuk, Jingjing Wu, Christoph S. Nabzdyk, and Xuanhe Zhao. "Instant tough bioadhesive with triggerable benign detachment." Proceedings of the National Academy of Sciences 117, no. 27 (June 23, 2020): 15497–503. http://dx.doi.org/10.1073/pnas.2006389117.

Full text

Abstract:

Bioadhesives such as tissue adhesives, hemostatic agents, and tissue sealants have potential advantages over sutures and staples for wound closure, hemostasis, and integration of implantable devices onto wet tissues. However, existing bioadhesives display several limitations including slow adhesion formation, weak bonding, low biocompatibility, poor mechanical match with tissues, and/or lack of triggerable benign detachment. Here, we report a bioadhesive that can form instant tough adhesion on various wet dynamic tissues and can be benignly detached from the adhered tissues on demand with a biocompatible triggering solution. The adhesion of the bioadhesive relies on the removal of interfacial water from the tissue surface, followed by physical and covalent cross-linking with the tissue surface. The triggerable detachment of the bioadhesive results from the cleavage of bioadhesive’s cross-links with the tissue surface by the triggering solution. After it is adhered to wet tissues, the bioadhesive becomes a tough hydrogel with mechanical compliance and stretchability comparable with those of soft tissues. We validate in vivo biocompatibility of the bioadhesive and the triggering solution in a rat model and demonstrate potential applications of the bioadhesive with triggerable benign detachment in ex vivo porcine models.

APA, Harvard, Vancouver, ISO, and other styles

42

Biswas, Deblina, George C. K. Chen, Hyoung Won Baac, and Srivathsan Vasudevan. "Photoacoustic Spectral Sensing Technique for Diagnosis of Biological Tissue Coagulation: In-Vitro Study." Diagnostics 10, no. 3 (February 29, 2020): 133. http://dx.doi.org/10.3390/diagnostics10030133.

Full text

Abstract:

Thermal coagulation of abnormal tissues has evolved as a therapeutic technique for different diseases including cancer. Tissue heating beyond 55 °C causes coagulation that leads to cell death. Noninvasive diagnosis of thermally coagulated tissues is pragmatic for performing efficient therapy as well as reducing damage of surrounding healthy tissues. We propose a noninvasive, elasticity-based photoacoustic spectral sensing technique for differentiating normal and coagulated tissues. Photoacoustic diagnosis is performed for quantitative differentiation of normal and coagulated excised chicken liver and muscle tissues in vitro by characterizing a dominant frequency of photoacoustic frequency spectrum. Pronounced distinction in the spectral parameter (i.e., dominant frequency) was observed due to change in tissue elastic property. We confirmed nearly two-fold increase in dominant frequencies for the coagulated muscle and liver tissues as compared to the normal ones. A density increase caused by tissue coagulation is clearly reflected in the dominant frequency composition. Experimental results were consistent over five different sample sets, delineating the potential of proposed technique to diagnose biological tissue coagulation and thus monitor thermal coagulation therapy in clinical applications.

APA, Harvard, Vancouver, ISO, and other styles

43

Marolla, Ana Paula Cleto, Jaques Waisberg, Gabriela Tognini Saba, Daniel Reis Waisberg, Fernando Beani Margeotto, and Maria Aparecida da Silva Pinhal. "Glycomics expression analysis of sulfated glycosaminoglycans of human colorectal cancer tissues and non-neoplastic mucosa by electrospray ionization mass spectrometry." Einstein (São Paulo) 13, no. 4 (December 2015): 510–17. http://dx.doi.org/10.1590/s1679-45082015ao3477.

Full text

Abstract:

ABSTRACT Objective To determine the presence of glycosaminoglycans in the extracellular matrix of connective tissue from neoplastic and non-neoplastic colorectal tissues, since it has a central role in tumor development and progression. Methods Tissue samples from neoplastic and non-neoplastic colorectal tissues were obtained from 64 operated patients who had colorectal carcinoma with no distant metastases. Expressions of heparan sulphate, chondroitin sulphate, dermatan sulphate and their fragments were analyzed by electrospray ionization mass spectrometry, with the technique for extraction and quantification of glycosaminoglycans after proteolysis and electrophoresis. The statistical analysis included mean, standard deviation, and Student’st test. Results The glycosaminoglycans extracted from colorectal tissue showed three electrophoretic bands in agarose gel. Electrospray ionization mass spectrometry showed characteristic disaccharide fragments from glycosaminoglycans, indicating their structural characterization in the tissues analyzed. Some peaks in the electrospray ionization mass spectrometry were not characterized as fragments of sugars, indicating the presence of fragments of the protein structure of proteoglycans generated during the glycosaminoglycan purification. The average amount of chondroitin and dermatan increased in the neoplastic tissue compared to normal tissue (p=0.01). On the other hand, the average amount of heparan decreased in the neoplastic tissue compared to normal tissue (p= 0.03). Conclusion The method allowed the determination of the glycosaminoglycans structural profile in colorectal tissue from neoplastic and non-neoplastic colorectal tissue. Neoplastic tissues showed greater amounts of chondroitin sulphate and dermatan sulphate compared to non-neoplastic tissues, while heparan sulphate was decreased in neoplastic tissues.

APA, Harvard, Vancouver, ISO, and other styles

44

Dzobo, Kevin, Nicholas Ekow Thomford, Dimakatso Alice Senthebane, Hendrina Shipanga, Arielle Rowe, Collet Dandara, Michael Pillay, and Keolebogile Shirley Caroline M. Motaung. "Advances in Regenerative Medicine and Tissue Engineering: Innovation and Transformation of Medicine." Stem Cells International 2018 (July 30, 2018): 1–24. http://dx.doi.org/10.1155/2018/2495848.

Full text

Abstract:

Humans and animals lose tissues and organs due to congenital defects, trauma, and diseases. The human body has a low regenerative potential as opposed to the urodele amphibians commonly referred to as salamanders. Globally, millions of people would benefit immensely if tissues and organs can be replaced on demand. Traditionally, transplantation of intact tissues and organs has been the bedrock to replace damaged and diseased parts of the body. The sole reliance on transplantation has created a waiting list of people requiring donated tissues and organs, and generally, supply cannot meet the demand. The total cost to society in terms of caring for patients with failing organs and debilitating diseases is enormous. Scientists and clinicians, motivated by the need to develop safe and reliable sources of tissues and organs, have been improving therapies and technologies that can regenerate tissues and in some cases create new tissues altogether. Tissue engineering and/or regenerative medicine are fields of life science employing both engineering and biological principles to create new tissues and organs and to promote the regeneration of damaged or diseased tissues and organs. Major advances and innovations are being made in the fields of tissue engineering and regenerative medicine and have a huge impact on three-dimensional bioprinting (3D bioprinting) of tissues and organs. 3D bioprinting holds great promise for artificial tissue and organ bioprinting, thereby revolutionizing the field of regenerative medicine. This review discusses how recent advances in the field of regenerative medicine and tissue engineering can improve 3D bioprinting and vice versa. Several challenges must be overcome in the application of 3D bioprinting before this disruptive technology is widely used to create organotypic constructs for regenerative medicine.

APA, Harvard, Vancouver, ISO, and other styles

45

Brandao, Mariana P., Ricardo Iwakura, Abraao A. Honorato-Sobrinho, Kaique Haleplian, Amando S. Ito, Luiz C. Conti de Freitas, and Luciano Bachmann. "Optical Characterization of Parathyroid Tissues." Applied Spectroscopy 70, no. 10 (July 20, 2016): 1709–16. http://dx.doi.org/10.1177/0003702816641120.

Full text

Abstract:

The parathyroid glands are small and often similar to lymph nodes, fat, and thyroid tissue. These glands are difficult to identify during surgery and a biopsy of the parathyroid for identification can lead to damage of the gland. The use of static and time-resolved fluorescence techniques to detect biochemical composition and tissue structure alterations could help to develop a portable, minimally invasive, and nondestructive method to assist medical evaluation of parathyroid tissues. In this study, we investigated 10 human parathyroid samples using absorbance, fluorescence, excitation, and time-resolved fluorescence measurements. Moreover, we compared the results of time-resolved fluorescence measurements with 59 samples of thyroid tissues. The fluorescence lifetimes with emission at 340 nm were 1.09 ± 0.10 and 4.46 ± 0.06 ns for healthy tissue, 1.01 ± 0.25 and 4.39 ± 0.36 ns for benign lesions, and 0.67 ± 0.36 and 3.92 ± 0.72 ns for malignant lesions. The lifetimes for benign and malignant lesions were significantly different, as attested by the analysis of variance with confidence levels higher than 87%. For each class of samples (healthy, benign, and malignant) we perceived statistical differences between the thyroid and parathyroid tissue, independently. After further investigations, fluorescence methods could become a tool to identify normal and pathological parathyroid tissues and distinguish thyroid from parathyroid tissues.

APA, Harvard, Vancouver, ISO, and other styles

46

Ganjewala, Deepak, Asha Devi S., and Ashwani Kumar Srivastava. "Tissue specific variation in biochemical compositions of Acorus calamus (L.) leaves and rhizomes." International Journal of Plant Biology 2, no. 1 (December 5, 2011): 4. http://dx.doi.org/10.4081/pb.2011.e4.

Full text

Abstract:

Sweet Flag (<em>Acorus calamus L</em>.) leaf and rhizome tissues were analyzed for biochemical compositions notably of carbohydrates and lipids. The glycolipid content measured in rhizome tissue was 62.3mg%/FW almost double the glycolipid content (28.8 mg%/FW) in leaf tissues, whereas the sterol content in the leaf tissue (47.9 mg%/FW) was three times of the sterol content in rhizome tissues (15.5 mg%/FW). Carbohydrates content such as total sugar, reducing sugar, sucrose and fructose measured in leaf and rhizome tissues were more or less similar, with slightly higher values of total sugar (18.2 mg%/FW) in the leaf tissues. The study thus revealed variation in biochemical compositions in two different tissues leaf and rhizome of A. calamus.

APA, Harvard, Vancouver, ISO, and other styles

47

Li, Jun, Zhong Zhong, Roy Lidtke, Klaus E. Kuettner, Charles Peterfy, Elmira Aliyeva, and Carol Muehleman. "Radiography of Soft Tissue of the Foot and Ankle with Diffraction Enhanced Imaging." Journal of the American Podiatric Medical Association 94, no. 3 (May 1, 2004): 315–22. http://dx.doi.org/10.7547/0940315.

Full text

Abstract:

Non-calcified tissues, including tendons, ligaments, adipose tissue and cartilage, are not visible, for any practical purposes, with conventional X-ray imaging. Therefore, any pathological changes in these tissues generally necessitate detection through magnetic resonance imaging or ultrasound technology. Until recently the development of an X-ray imaging technique that could detect both bone and soft tissues seemed unrealistic. However, the introduction of diffraction enhanced X-ray imaging (DEI) which is capable of rendering images with absorption, refraction and scatter rejection qualities has allowed detection of specific soft tissues based on small differences in tissue densities. Here we show for the first time that DEI allows high contrast imaging of soft tissues, including ligaments, tendons and adipose tissue, of the human foot and ankle. (J Am Podiatr Med Assoc 94(3): 315–322, 2004)

APA, Harvard, Vancouver, ISO, and other styles

48

Li, Weiwei, and Min Liu. "Distribution of 5-Hydroxymethylcytosine in Different Human Tissues." Journal of Nucleic Acids 2011 (2011): 1–5. http://dx.doi.org/10.4061/2011/870726.

Full text

Abstract:

5-hydroxymethylcytosine (5-hmC) is a modified form of cytosine recently found in mammalians and is believed, like 5-methylcytosine, to also play an important role in switching genes on and off. By utilizing a newly developed 5-hmC immunoassay, we determined the abundance of 5-hmC in human tissues and compared 5-hmC states in normal colorectal tissue and cancerous colorectal tissue. Significant differences of 5-hmC content in different tissues were observed. The percentage of 5-hmC measured is high in brain, liver, kidney and colorectal tissues (0.40–0.65%), while it is relatively low in lung (0.18%) and very low in heart, breast, and placenta (0.05-0.06%). Abundance of 5-hmC in the cancerous colorectal tissues was significantly reduced (0.02–0.06%) compared to that in normal colorectal tissues (0.46–0.57%). Our results showed for the first time that 5-hmC distribution is tissue dependent in human tissues and its abundance could be changed in the diseased states such as colorectal cancer.

APA, Harvard, Vancouver, ISO, and other styles

49

Cotton, D. W. K., and T. J. Stephenson. "Impairment of Autopsy Histology by Organ Washinga Myth." Medicine, Science and the Law 28, no. 4 (October 1988): 319–23. http://dx.doi.org/10.1177/002580248802800411.

Full text

Abstract:

ABSTRACT: Histological sections of post-mortem tissues are often poorly preserved and many pathologists feel that washing tissues during the autopsy may contribute to this. This proposition was tested by immersing blocks of fresh tissue in tap water for 10 minutes and assessing the histological preservation of subsequent tissue sections. Blocks of liver were also kept in tap water for periods of up to 34 hours and the amount of tissue damage was assessed using routine histology. All slides were coded and interpreted by an observer who had not previously seen the tissues. Photographs of the various tissues stained with a variety of techniques were also offered to a group of pathologists, at a Pathological Society meeting, who also attempted to classify them as ‘washed’ or ‘unwashed’. The results were statistically random and showed that washing tissues in the manner described had no deleterious effect on subsequent histological preparations. We conclude that there is no reason to avoid washing tissues during the routine autopsy.

APA, Harvard, Vancouver, ISO, and other styles

50

White, Eoin J., Eoghan M. Cunnane, Muireann McMahon, Michael T. Walsh, J. Calvin Coffey, and Leonard O’Sullivan. "Mechanical characterisation of porcine non-intestinal colorectal tissues for innovation in surgical instrument design." Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine 232, no. 8 (July 23, 2018): 796–806. http://dx.doi.org/10.1177/0954411918788595.

Full text

Abstract:

This article presents an investigation into the mechanical properties of porcine mesocolon, small intestinal mesentery, fascia, and peritoneum tissues to generate a preliminary database of the mechanical characteristics of these tissues as surrogates for human tissue. No study has mechanically characterised porcine tissue correlates of the mesentery and associated structures. The samples were tested to determine the strength, stretch at failure, and stiffness of each tissue. The results indicated that porcine mesenteric and associated tissues visually resembled corresponding human tissues and had similar tactile characteristics, according to an expert colorectal surgeon. Stiffness values ranged from 0.088 MPa to 6.858 MPa across all tissues, with fascia being the weakest, and mesentery and peritoneum being the strongest. Failure stress values ranged from 0.336 MPa to 6.517 MPa, and failure stretch values ranged from 1.766 to 3.176, across all tissues. These mechanical data can serve as reference baseline data upon which future work can expand.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Tissues'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles