Relevant bibliographies by topics / Tissues Cryopreservation

Academic literature on the topic 'Tissues Cryopreservation'

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Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Tissues Cryopreservation"

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Crowley, Conor A., William P. W. Smith, K. T. Matthew Seah, Soo-Keat Lim, and Wasim S. Khan. "Cryopreservation of Human Adipose Tissues and Adipose-Derived Stem Cells with DMSO and/or Trehalose: A Systematic Review." Cells 10, no. 7 (July 20, 2021): 1837. http://dx.doi.org/10.3390/cells10071837.

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Adipose tissue senescence is implicated as a major player in obesity- and ageing-related disorders. There is a growing body of research studying relevant mechanisms in age-related diseases, as well as the use of adipose-derived stem cells in regenerative medicine. The cell banking of tissue by utilising cryopreservation would allow for much greater flexibility of use. Dimethyl sulfoxide (DMSO) is the most commonly used cryopreservative agent but is toxic to cells. Trehalose is a sugar synthesised by lower organisms to withstand extreme cold and drought that has been trialled as a cryopreservative agent. To examine the efficacy of trehalose in the cryopreservation of human adipose tissue, we conducted a systematic review of studies that used trehalose for the cryopreservation of human adipose tissues and adipose-derived stem cells. Thirteen articles, including fourteen studies, were included in the final review. All seven studies that examined DMSO and trehalose showed that they could be combined effectively to cryopreserve adipocytes. Although studies that compared nonpermeable trehalose with DMSO found trehalose to be inferior, studies that devised methods to deliver nonpermeable trehalose into the cell found it comparable to DMSO. Trehalose is only comparable to DMSO when methods are devised to introduce it into the cell. There is some evidence to support using trehalose instead of using no cryopreservative agent.
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Arav, Amir. "Cryopreservation by Directional Freezing and Vitrification Focusing on Large Tissues and Organs." Cells 11, no. 7 (March 22, 2022): 1072. http://dx.doi.org/10.3390/cells11071072.

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The cryopreservation of cells has been in routine use for decades. However, despite the extensive research in the field, cryopreservation of large tissues and organs is still experimental. The present review highlights the major studies of directional freezing and vitrification of large tissues and whole organs and describes the different parameters that impact the success rate of large tissue and organ cryopreservation. Key factors, such as mass and heat transfer, cryoprotectant toxicity, nucleation, crystal growth, and chilling injury, which all have a significant influence on whole-organ cryopreservation outcomes, are reviewed. In addition, an overview of the principles of directional freezing and vitrification is given and the manners in which cryopreservation impacts large tissues and organs are described in detail.
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Müller-Schweinitzer, Else. "Cryopreservation of vascular tissues." Organogenesis 5, no. 3 (July 2009): 97–104. http://dx.doi.org/10.4161/org.5.3.9495.

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Xu, Feng, Sangjun Moon, Xiaohui Zhang, Lei Shao, Young Seok Song, and Utkan Demirci. "Multi-scale heat and mass transfer modelling of cell and tissue cryopreservation." Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 368, no. 1912 (February 13, 2010): 561–83. http://dx.doi.org/10.1098/rsta.2009.0248.

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Cells and tissues undergo complex physical processes during cryopreservation. Understanding the underlying physical phenomena is critical to improve current cryopreservation methods and to develop new techniques. Here, we describe multi-scale approaches for modelling cell and tissue cryopreservation including heat transfer at macroscale level, crystallization, cell volume change and mass transport across cell membranes at microscale level. These multi-scale approaches allow us to study cell and tissue cryopreservation.
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Lee, Sanghoon, Ki-Jin Ryu, Boram Kim, Dahyeon Kang, Yoon Young Kim, and Tak Kim. "Comparison between Slow Freezing and Vitrification for Human Ovarian Tissue Cryopreservation and Xenotransplantation." International Journal of Molecular Sciences 20, no. 13 (July 8, 2019): 3346. http://dx.doi.org/10.3390/ijms20133346.

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Two methods for the cryopreservation of human ovarian tissue were compared using a xenotransplantation model to establish a safe and effective cryopreservation method. Ovarian tissues were obtained from women who underwent benign ovarian surgery in the gynecology research unit of a university hospital. The tissues were transplanted into 112 ovariectomized female severe combined immunodeficient mice 4 weeks after slow freezing or vitrification cryopreservation. Tissues were retrieved 4 weeks later. Primordial follicular counts decreased after cryopreservation and xenotransplantation, and were significantly higher in the slow freezing group than in the vitrification group (p < 0.001). Immunohistochemistry and TUNEL assay showed that the Ki-67 and CD31 markers of follicular proliferation and angiogenesis were higher in the slow freezing group (p < 0.001 and p = 0.006, respectively) and DNA damage was greater in the vitrification group (p < 0.001). Western blotting showed that vitrification increased cellular apoptosis. Anti-Müllerian hormone expression was low in transplanted samples subjected to both cryopreservation techniques. Electron microscopy revealed primordial follicle deformation in the vitrification group. Slow freezing for ovarian tissue cryopreservation is superior to vitrification in terms of follicle survival and growth after xenotransplantation. These results will be useful for fertility preservation in female cancer patients.
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Bakhach, Joseph. "The cryopreservation of composite tissues." Organogenesis 5, no. 3 (July 2009): 119–26. http://dx.doi.org/10.4161/org.5.3.9583.

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Cui, X. D., D. Y. Gao, B. F. Fink, H. C. Vasconez, and L. L. Q. Pu. "Cryopreservation of human adipose tissues." Cryobiology 55, no. 3 (December 2007): 269–78. http://dx.doi.org/10.1016/j.cryobiol.2007.08.012.

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Hughes, Sean M., April L. Ferre, Sarah E. Yandura, Cory Shetler, Chris A. R. Baker, Fernanda Calienes, Claire N. Levy, et al. "Cryopreservation of human mucosal tissues." PLOS ONE 13, no. 7 (July 30, 2018): e0200653. http://dx.doi.org/10.1371/journal.pone.0200653.

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Islam, Nadia, Ugwoke Sunday Paul, Rana Alhamdan, Juan Hernandez-Medrano, Bruce K. Campbell, Peter Marsters, and Walid E. Maalouf. "Steroids and miRNAs in assessment of ovarian tissue damage following cryopreservation." Journal of Molecular Endocrinology 62, no. 4 (May 2019): 207–16. http://dx.doi.org/10.1530/jme-18-0237.

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Ovarian cortical tissue cryopreservation is a relatively novel approach to preserving fertility in women diagnosed with cancer. However, the effects of freezing-thawing are not fully understood, mainly due to the lack of suitable methods to assess tissue’s survival after thawing. Disparities in steroid production have been associated with ovarian failure by disrupting folliculogenesis, ovulation and oocyte apoptosis. Moreover, specific miRNAs, identified in human ovarian follicles, are thought to play a fundamental role in folliculogenesis. In this study, we investigated the possible interplay between the ovarian steroidal production and miRNA expression patterns in spent culture media, as potential non-invasive markers for ovarian tissue damage after cryopreservation. Cryopreservation of ovarian cortical tissue decreased (P < 0.05) both steroid production (oestradiol and progesterone) and expression of miRNA-193b and 320A in spent culture media over 5 days; however, expression of miRNA-24 increased (P < 0.05). The number of primordial follicles was also reduced (P < 0.05) in fresh-cultured and cryopreserved-cultured cortical tissues when compared with fresh tissues. Downregulation of miRNA-193b and miRNA-320A together with upregulation of miRNA-24 could have a synergistic role in cell apoptosis, and consequently leading to reduced oestradiol and progesterone production. Thus, there appears to be an interplay between these miRNAs, ovarian steroid production and cell damage, which can be further explored as novel non-invasive markers of cell damage following cryopreservation.
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Matsuura, Yoshitaka, Michiharu Sakamoto, Shuichi Ogino, Jun Arata, and Naoki Morimoto. "Inactivated Nevus Tissue with High Hydrostatic Pressure Treatment Used as a Dermal Substitute after a 28-Day Cryopreservation Period." BioMed Research International 2021 (February 24, 2021): 1–9. http://dx.doi.org/10.1155/2021/3485189.

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Background. Giant congenital melanocytic nevi (GCMN) treatment remains controversial. While surgical resection is the best option for complete removal, skin shortage to reconstruct the skin defect remains an issue. We report a novel treatment using a high hydrostatic pressurization (HHP) technique and a cryopreservation procedure. However, cryopreservation may inhibit revascularization of implanted nevus tissue and cultured epidermal autograft (CEA) take. We aimed to investigate the influence of the cryopreservation procedure on the HHP-treated dermis specimen and CEA take on cryopreserved tissue. Methods. Nevus tissue harvested from a patient with GCMN was inactivated with HHP of 200 MPa and then cryopreserved at -30°C for 28 days. The cryopreserved specimen was compared with fresh (HHP-treated without cryopreservation) tissue and with untreated (without HHP treatment) tissue to evaluate the extracellular matrix, basal membranes, and capillaries. Cultured epidermis (CE) take on the cryopreserved tissue was evaluated following implantation of the cryopreserved nevus tissue with CE into the subcutis of nude mice. Results. No difference was observed between cryopreserved and fresh tissue in terms of collagen or elastic fibers, dermal capillaries, or basement membranes at the epidermal-dermal junction. In 4 of 6 samples (67%), applied CE took on the nevus tissues and regenerated the epidermis in the cryopreserved group compared with 5 of 6 samples (83%) in the fresh group. Conclusion. Cryopreservation at -30°C for 28 days did not result in significant damage to inactivated nevus tissue, and applied CE on the cryopreserved nevus tissues took and regenerated the epidermis. Inactivated nevus tissue with HHP can be used as a dermal substitute after 28-day cryopreservation.
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Dissertations / Theses on the topic "Tissues Cryopreservation"

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Beaty, Myron H. "Cryopreservation of eukaryote algae." Thesis, This resource online, 1991. http://scholar.lib.vt.edu/theses/available/etd-07282008-135156/.

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Vogel, Martin Joseph. "Proteomic profiling following cryopreservation." Diss., Online access via UMI:, 2004. http://wwwlib.umi.com/dissertations/fullcit/1424168.

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Mukherjee, Indra Neil. "A rational design approach for the cryopreservation of natural and engineered tissues." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/22579.

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Thesis (Ph. D.)--Chemical and Biomolecular Engineering, Georgia Institute of Technology, 2008.
Committee Chair: Sambanis, Athanassios; Committee Member: Long, Jr., Robert C.; Committee Member: Ludovice, Peter J.; Committee Member: Prausnitz, Mark R.; Committee Member: Song, Ying C.
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Xu, Xia. "A study of mass transfer in cryopreservation of living tissues." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275199.

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Els, Cecilia Lydia. "Early human follicle ultrastructure comparison after slow cryopreservation in two different cryoprotectants." Thesis, Link to the online version, 2008. http://hdl.handle.net/10019/831.

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Higgins, Adam Zachary. "Intracellular ice formation in tissue constructs and the effects of mass transport across the cell membrane." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/28166.

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Thesis (M. S.)--Biomedical Engineering, Georgia Institute of Technology, 2008.
Committee Chair: Karlsson, Jens; Committee Co-Chair: Nerem, Robert; Committee Member: Meda, Paolo; Committee Member: Prausnitz, Mark; Committee Member: Sands, Jeff; Committee Member: Zhu, Cheng.
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Kagawa, Keiko Sompop Prathanturarug. "Cryopreservation of dendrobium cruentum Rchb. f. /." Abstract, 2006. http://mulinet3.li.mahidol.ac.th/thesis/2549/cd394/4738650.pdf.

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Bedaiwy, Mohamed Ali. "Ovarian tissue cryopreservation and transplantation : approaches and techniques /." Cleveland, Ohio : Cleveland Clinic, 2007. http://www.loc.gov/catdir/toc/ecip082/2007042633.html.

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Fuku, Eiji. "Studies on the cryopreservation of immature and in vitro matured bovine - oocytes." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41590.

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The developmental potential of mammalian oocytes cryopreserved with procedures similar to those used for embryos has been limited, inasmuch as oocytes differ from embryos in advanced stages of development, both physiologically and morphologically. The objective of this work was to elucidate the precise nature of freeze-thaw damage with the expectation that identification of specific targets will enable devising optimal procedures for cryopreservation of bovine oocytes to prevent specific damage and minimize the loss of developmental capacity.
In the first series of experiments, bovine oocytes were vitrified (V-oocytes) or frozen slowly (S-oocytes) at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Survival was assessed morphologically and also by in vitro fertilization (IVF) and culture (IVC). Morphological integrity and developmental capacity were greater in S-oocytes than in V-oocytes (P $<$ 0.05). Transfer of four embryos (2 morulae and 2 blastocysts) derived from post-IVM S-oocytes into a recipient heifer resulted in the birth of twin calves.
In the second series of experiments, oocytes (GV and IVM) were exposed to a cryoprotectant solution (DAP213: 2M DMSO, 1M acetamide, 3M propanediol) for 1.5 or 5 min and viability assessed by IVM-IVF-IVC. Oocytes were also examined by transmission electron microscopy (TEM) before (control) or after exposure to the cryoprotectant. DAP213 induced profound premature cortical granule (CG) release and vesiculation. These changes were less pronounced in oocytes exposed to DAP213 only after IVM. The results suggest that: (1) the extrusion of CG is one of the important cytological events affected by the treatment of oocytes with DAP213; (2) GV oocytes are more sensitive to the cryoprotectant than IVM oocytes.
In the third series of experiments, GV and IVM oocytes were vitrified with DAP213. On rewarming, DAP213 was removed by a one- or three-step dilution procedure and survival assessed by development after IVM-IVF-IVC. Morphology was assessed by TEM study immediately following DAP213 removal. Both assessments indicated that: (1) IVM oocytes are more tolerant to vitrification than are GV oocytes; (2) the three-step dilution is less damaging than the one-step procedure; (3) changes in the zona pellucida (loss of plasticity) of IVM oocytes following vitrification may result from the premature release of cortical granules.
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Lawson, Alison N. "Cryopreservation effects on the in vitro and in vivo function of a model pancreatic substitute." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/39540.

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The effects of two types of cryopreservation, conventional freezing and vitrification, on the in vitro and in vivo function of a pancreatic substitute were investigated. Conventional freezing uses low concentrations of cryoprotective agents (CPAs), slow cooling and rapid warming and allows ice formation. Vitrification requires high concentrations of CPAs coupled with rapid cooling and warming to achieve a vitreous, or ice-free, state. A previously published mathematical model describing the mass transfer of CPAs through the alginate matrix of the substitute and the cell membrane was expanded to incorporate heat transfer as well as CPA cytotoxicity. Our results indicate that temperature of exposure is the most critical parameter for the proper design of CPA addition and removal protocols. The use of a mathematical model is critical to ensure CPA equilibration and minimize CPA exposure. Properly designed CPA addition and removal protocols were used for vitrification. The effects of cryopreservation on the biomaterial and the cellular function of a pancreatic substitute consisting of murine insulinomas encapsulated in calcium alginate/poly-L-lysine/alginate beads were assessed. In vitro results indicate that both vitrification and conventionally frozen perform comparably to fresh. However, in vivo studies reveal that vitrified beads perform worse than both conventionally frozen and fresh beads. With adjustments, it may be possible to improve the performance of the vitrified beads. Nevertheless, for this pancreatic substitute, conventional freezing is the better method and allows successful cryopreservation.
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Books on the topic "Tissues Cryopreservation"

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E, Pegg David, Karow Armond M, and North Atlantic Treaty Organization. Scientific Affairs Division., eds. The biophysics of organ cryopreservation. New York: Published in cooperation with NATO Scientific Affairs Division [by] Plenum Press, 1987.

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Claeys, Cor. Cryopreservation and freeze-drying protocols. [Place of publication not identified]: Humana, 2010.

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Sauter, Peter Richard. Kryokonservierung von Lemnaceae =: Cryopreservation of Lemnaceae. Zürich: Geobotanischen Institut ETH, Stiftung Rübel, 1993.

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International Institute of Refrigeration. Commission C1. International Conference. Cryoprotectants in medical practice =: Utilisation des cryoprotecteurs en médecine : May 12-15, 1997, Hradec Králové (Czech Republic/Rép. Techèque). Paris, France: International Institute of Refrigeration, 1997.

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Moore, Alan A. Refrigerated storage and cryopreservation of walleye and muskellunge semen. [Des Moines, Iowa]: Iowa Dept. of Natural Resources, 1991.

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Echlin, Patrick. Low-temperature microscopy and analysis. New York: Plenum Press, 1992.

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American Society of Mechanical Engineers. Winter Meeting. Low temperature biotechnology: Emerging applications and engineering contributions. New York, NY (345 E. 47th St., New York 10017): ASME, 1988.

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Th, Smit Sibinga C., Das P. C, Meryman Harold Thayer, and Stichting Rode Kruis Bloedbank Groningen/Drente., eds. Cryopreservation and low temperature biology in blood transfusion: Proceedings of the Fourteenth International Symposium on Blood Transfusion, Groningen 1989. Dordrecht, The Netherlands: Kluwer Academic Publishers, 1990.

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Hopkins, R. A. (Richard A.)., ed. Cardiac reconstructions with allograft tissues. New York: Springer-Verlag, 2005.

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Attanasio, A. A. Solis. London: New English Library, 1994.

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Book chapters on the topic "Tissues Cryopreservation"

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Müller-Schweinitzer, E. "Cryopreservation of human pulmonary tissues." In Methods in Pulmonary Research, 509–21. Basel: Birkhäuser Basel, 1998. http://dx.doi.org/10.1007/978-3-0348-8855-4_20.

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Brockbank, Kelvin G. M., Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell. "Vitrification of Heart Valve Tissues." In Cryopreservation and Freeze-Drying Protocols, 399–421. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2193-5_20.

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Brockbank, Kelvin G. M., Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell. "Vitrification of Heart Valve Tissues." In Cryopreservation and Freeze-Drying Protocols, 593–605. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0783-1_31.

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Pegg, David E. "Ice Crystals in Tissues and Organs." In The Biophysics of Organ Cryopreservation, 117–40. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4684-5469-7_7.

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Wolfinbarger, Lloyd, Kelvin G. M. Brockbank, and Richard A. Hopkins. "Application of Cryopreservation to Heart Valves." In Cardiac Reconstructions with Allograft Tissues, 133–60. New York, NY: Springer New York, 2005. http://dx.doi.org/10.1007/0-387-26515-5_16.

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Wolkers, Willem F., and Andres Hilfiker. "Freeze-Drying of Decellularized Heart Valve Tissues." In Cryopreservation and Freeze-Drying Protocols, 499–506. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2193-5_26.

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Warner, Ross M., and Adam Z. Higgins. "Mathematical Modeling of Protectant Transport in Tissues." In Cryopreservation and Freeze-Drying Protocols, 173–88. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0783-1_5.

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Wu, Kezhou, Leila Laouar, Nadia Shardt, Janet A. W. Elliott, and Nadr M. Jomha. "Osmometric Measurements of Cryoprotective Agent Permeation into Tissues." In Cryopreservation and Freeze-Drying Protocols, 303–15. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0783-1_11.

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Häggman, Hely, Mari Rusanen, and Soile Jokipii. "Cryopreservation of In Vitro Tissues of Deciduous Forest Trees." In Plant Cryopreservation: A Practical Guide, 365–86. New York, NY: Springer New York, 2008. http://dx.doi.org/10.1007/978-0-387-72276-4_14.

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Wolkers, Willem F., and Harriëtte Oldenhof. "Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues." In Cryopreservation and Freeze-Drying Protocols, 3–25. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0783-1_1.

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Conference papers on the topic "Tissues Cryopreservation"

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Seawright, Angela, Altug Ozcelikkale, J. Craig Dutton, and Bumsoo Han. "Role of Cells in Freezing-Induced Cell-Fluid Matrix Interactions Within Engineered Tissues." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80531.

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Cryopreservation can provide long-term storage of various biological tissues, which has significant impact on tissue engineering and regenerative medicine. For successful cryopreservation of tissues, tissue functionality must be maintained including physical properties such as mechanical, optical, and transport properties, as well as cellular viability. Such properties are associated with the extracellular matrix (ECM) microstructure. Thus, the preservation of the ECM microstructure may lead to successful cryopreservation [1,2]. Yet, there is still very little known about changes in the ECM microstructure during freezing/thawing.
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Rabin, Yoed, Justin S. G. Feig, Alexander C. Williams, Christopher C. Lin, and Chandrajit Thaokar. "Cryomacroscopy in 3D: A Device Prototype for the Study of Cryopreservation." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80527.

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This study presents a new device prototype for visualization of physical effects associated with large-scale cryopreservation—the preservation of tissues at very low temperatures. Cryopreservation represents the only method for long-term preservation of biomaterials. While techniques for cryopreservation of single cells and small tissue structures are well established, cryopreservation techniques for bulky tissues and organs are still at the developmental stage. Critical to the success of cryopreservation is the control of ice formation—the cornerstone of cryoinjury. One of the most promising techniques for large-scale cryopreservation is known as vitrification, where the crystal phase is suppressed, and the biological material is trapped in a glassy-like state (vitreous in Latin means glassy) [1].
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Teo, Ka Yaw, J. Craig Dutton, and Bumsoo Han. "The Effects of Cryoprotective Agent on Spatiotemporal Deformation of Engineered Tissues During Freezing." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19277.

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Maintaining tissue functionality is crucial for successful cryopreservation. However, current understanding of the effects of cryopreservation including those of cryoprotective agents (CPAs) on post-thaw tissue functionality is still very limited. In numerous cryopreservation protocols, CPAs such as dimethyl sulfoxide (DMSO) are commonly used. Different concentrations and combinations of CPAs have been reported to be advantageous in maintaining post-thaw tissue functionality, but the determination of their optimum composition often requires extensive experimental studies due to the lack of understanding of their effects on the biophysics of the tissues during freezing.
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Teo, Ka Yaw, J. Craig Dutton, Frederick Grinnell, and Bumsoo Han. "Effects of Freezing-Induced Cell-Fluid-Matrix Interactions on Cells and Extracellular Matrix of Engineered Tissues." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53407.

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Long-term cryopreservation of functional engineered tissues (ETs) is a key enabling technology for tissue engineering and regenerative medicine. However, a limited understanding of tissue-level biophysical phenomena during freeze/thaw (F/T) and their effects on cells and ECM microstructure poses significant challenges for i) preserving tissue functionality, and ii) controlling highly tissue-type dependent cryopreservation outcomes.
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Seawright, Angela, and Bumsoo Han. "Effects of Freezing on Cytoskeletal Structure of Fibroblasts in Engineered Tissues." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53105.

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Successful preservation of functional cells and tissues are critical to cell/tissue engineering and regenerative medicines. One of the most reliable methods of cell/tissue preservation is cryopreservation; in which cells and tissues are frozen before storage and thawed when needed. Since the cellular viability and the microstructure of extracellular matrix (ECM) directly correspond to the functionality of a tissue, successful cryopreservation should maintain the extracellular matrix (ECM) microstructure as well as cellular viability post-thaw. A few recent studies [1,2] reported that complex cell-fluid-matrix interaction occurred during freezing of tissues, and this interaction might determine the post-thaw ECM microstructure and affect the cellular viability. These studies also suggested that the cells within tissues might exert or sense mechanical stress during freezing through the cytoskeleton connected to the ECM via cell-matrix adhesion complexes.
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Liu, B. L., and J. J. McGrath. "Ice Formation of Vitrification Solutions for Cryopreservation of Tissues." In 2005 IEEE Engineering in Medicine and Biology 27th Annual Conference. IEEE, 2005. http://dx.doi.org/10.1109/iembs.2005.1616247.

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Ozcelikkale, Altug, Yan Li, Xianfan Xu, and Bumsoo Han. "Effects of Freezing on Collagen Nanoscale Structure in Engineered Tissues." In ASME 2013 2nd Global Congress on NanoEngineering for Medicine and Biology. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/nemb2013-93125.

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The present study aims to systematically investigate the freezing-induced changes that occur at multiple levels of organization of collagen nanostructure in the engineered tissues (ET). Collagen is a major constituent of the extracellular matrix (ECM) of biological tissues, and is also used for scaffold of engineered tissues and biomaterials [1, 2]. Given its abundance and widespread physiological function in vivo, a proper understanding of the relationships between the collagen’s structure, properties, and function is essential for the improvement of current tissue cryopreservation protocols that suffer from highly variable and tissue specific outcomes [3, 4].
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8

Applegate, Dawn R., Kang Liu, and Jonathan Mansbridge. "Practical Considerations for Large-Scale Cryopreservation of a Tissue Engineered Human Dermal Replacement." In ASME 1999 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/imece1999-0587.

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Abstract Tissue engineering is redefining the field of transplantation by providing a readily available, off-the-shelf supply of consistent, easy to use, safe and effective products. Successful design and scale-up of both tissue growth and preservation processes have enabled maintenance of tissue integrity, functionality and viability from product manufacture to end-use and afforded clinical efficacy, feasibility of large-volume distribution and cost-effectiveness. Advances in tissue preservation are being realized through extension of mathematical models and biological principles for isolated cells to bench-scale tissue systems. Hence, implementation problems inherent to large-scale systems are not often considered. Furthermore, the effects of preservation on tissues are not always tested in vivo. This paper addresses the practical obstacles to the design and implementation of a large-scale cryopreservation process. The effects of cryopreservation on in vivo tissue functionality including alteration of cell signal transduction pathways and expression of stress proteins in response to cryopreservation are also reviewed.
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9

Irimia, Daniel, and Jens O. M. Karlsson. "Monte Carlo Simulation of Ice Formation in Tissues." In ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-32681.

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Freezing is a common technique for preservation of isolated cells, and extending its applications to the preservation of tissues would have important implications for the storage and distribution of tissue engineered products. Unlike isolated cells in suspension, cells in tissue interact with each other, and this interaction is known to affect the outcome of tissue cryopreservation. As a consequence, our knowledge of the cryobiology of isolated cells cannot simply be extrapolated to tissues, and new models, which consider the interaction between cells, need to be developed. The model that we propose is based on previous quantitative analysis of intercellular ice propagation in a micropatterned two-cell system. We used Monte Carlo simulations to extrapolate the results from cell pairs to two-dimensional and three-dimensional tissues. Effects of tissue geometry, cellular connectivity, and degree of intercellular interaction were investigated.
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10

Choi, Jeunghwan, and John C. Bischof. "Attachment State Shifts Viability Versus Cooling Rate (Inverted U Curve) During Freezing for Human Dermal Fibroblasts." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53156.

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A large number of studies in cryobiology have focused on understanding the underlying biophysics at the cellular level to help predict survival outcome after cryopreservation or cryosurgery. While this behavior is increasingly well studied and understood in cells gaps remain in our understanding of how cells in tissues behave which can hamper freezing applications in tissues. This study compares freezing behavior in cells in suspension vs. attached (a model tissue) state to investigate any differences in cellular behavior in these two states.
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Reports on the topic "Tissues Cryopreservation"

1

SAMBANIS, ATHANASSIOS. Final Report on Cryopreservation of Biological Tissues. Office of Scientific and Technical Information (OSTI), June 2001. http://dx.doi.org/10.2172/782715.

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