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Journal articles on the topic 'Tissue engineering. Regenerative Medicine'

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Author: Grafiati
Published: 14 March 2023

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1

Dzobo, Kevin, Nicholas Ekow Thomford, Dimakatso Alice Senthebane, Hendrina Shipanga, Arielle Rowe, Collet Dandara, Michael Pillay, and Keolebogile Shirley Caroline M. Motaung. "Advances in Regenerative Medicine and Tissue Engineering: Innovation and Transformation of Medicine." Stem Cells International 2018 (July 30, 2018): 1–24. http://dx.doi.org/10.1155/2018/2495848.

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Humans and animals lose tissues and organs due to congenital defects, trauma, and diseases. The human body has a low regenerative potential as opposed to the urodele amphibians commonly referred to as salamanders. Globally, millions of people would benefit immensely if tissues and organs can be replaced on demand. Traditionally, transplantation of intact tissues and organs has been the bedrock to replace damaged and diseased parts of the body. The sole reliance on transplantation has created a waiting list of people requiring donated tissues and organs, and generally, supply cannot meet the demand. The total cost to society in terms of caring for patients with failing organs and debilitating diseases is enormous. Scientists and clinicians, motivated by the need to develop safe and reliable sources of tissues and organs, have been improving therapies and technologies that can regenerate tissues and in some cases create new tissues altogether. Tissue engineering and/or regenerative medicine are fields of life science employing both engineering and biological principles to create new tissues and organs and to promote the regeneration of damaged or diseased tissues and organs. Major advances and innovations are being made in the fields of tissue engineering and regenerative medicine and have a huge impact on three-dimensional bioprinting (3D bioprinting) of tissues and organs. 3D bioprinting holds great promise for artificial tissue and organ bioprinting, thereby revolutionizing the field of regenerative medicine. This review discusses how recent advances in the field of regenerative medicine and tissue engineering can improve 3D bioprinting and vice versa. Several challenges must be overcome in the application of 3D bioprinting before this disruptive technology is widely used to create organotypic constructs for regenerative medicine.
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2

Sahoo, Sambit, Thomas KH Teh, Pengfei He, Siew Lok Toh, and James CH Goh. "Interface Tissue Engineering: Next Phase in Musculoskeletal Tissue Repair." Annals of the Academy of Medicine, Singapore 40, no. 5 (May 15, 2011): 245–51. http://dx.doi.org/10.47102/annals-acadmedsg.v40n5p245.

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Increasing incidence of musculoskeletal injuries coupled with limitations in the current treatment options have necessitated tissue engineering and regenerative medicine- based approaches. Moving forward from engineering isolated musculoskeletal tissues, research strategies are now being increasingly focused on repairing and regenerating the interfaces between dissimilar musculoskeletal tissues with the aim to achieve seamless integration of engineered musculoskeletal tissues. This article reviews the state-of-the-art in the tissue engineering of musculoskeletal tissue interfaces with a focus on Singapore’s contribution in this emerging field. Various biomimetic scaffold and cell-based strategies, the use of growth factors, gene therapy and mechanical loading, as well as animal models for functional validation of the tissue engineering strategies are discussed. Keywords: Functional tissue engineering, Orthopaedic interfaces, Regenerative medicine, Scaffolds
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3

SM, Harsini. "Bone Regenerative Medicine and Bone Grafting." Open Access Journal of Veterinary Science & Research 3, no. 4 (2018): 1–7. http://dx.doi.org/10.23880/oajvsr-16000167.

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Bone tissues can repair and regenerate it: in many clinical cases, bone fractures repair without scar formation. Nevertheless, in large bone defects and pathological fractures, bone healing fail to heal. Bone grafting is defined as implantation of material which promot es fracture healing, through osteoconduction osteogenesis, and osteoinduction. Ideal bone grafting depends on several factors such as defect size, ethical issues, biomechanical characteristics, tissue viability, shape and volume, associated complications, cost, graft size, graft handling, and biological characteristics. The materials that are used as bone graft can be divided into separate major categories, such as autografts, allografts, and xenografts. Synthetic substitutes and tissue - engineered biomateri als are other options. Each of these instances has some advantages and disadvantages. Between the all strategies for improving fracture healing and enhance the outcome of unification of the grafts, tissue engineering is a suitable option. A desirable tissu e - engineered bone must have properties similar to those of autografts without their limitations. None of the used bone grafts has all the ideal properties including low donor morbidity, long shelf life, efficient cost, biological safety, no size restrictio n, and osteoconductive, osteoinductive, osteogenic, and angiogenic properties; but Tissue engineering tries to supply most of these features. In addition it is able to induce healing and reconstruction of bone defects. Combining the basis of orthopedic sur gery with knowledge from different sciences like materials science, biology, chemistry, physics, and engineering can overcome the limitations of current therapies. Combining the basis of orthopedic surgery with knowledge from different sciences like materi als science, biology, chemistry, physics, and engineering can overcome the limitations of current therapies.
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Ribitsch, Iris, Gil Lola Oreff, and Florien Jenner. "Regenerative Medicine for Equine Musculoskeletal Diseases." Animals 11, no. 1 (January 19, 2021): 234. http://dx.doi.org/10.3390/ani11010234.

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Musculoskeletal injuries and chronic degenerative diseases commonly affect both athletic and sedentary horses and can entail the end of their athletic careers. The ensuing repair processes frequently do not yield fully functional regeneration of the injured tissues but biomechanically inferior scar or replacement tissue, causing high reinjury rates, degenerative disease progression and chronic morbidity. Regenerative medicine is an emerging, rapidly evolving branch of translational medicine that aims to replace or regenerate cells, tissues, or organs to restore or establish normal function. It includes tissue engineering but also cell-based and cell-free stimulation of endogenous self-repair mechanisms. Some regenerative medicine therapies have made their way into equine clinical practice mainly to treat tendon injures, tendinopathies, cartilage injuries and degenerative joint disorders with promising results. However, the qualitative and quantitative spatiotemporal requirements for specific bioactive factors to trigger tissue regeneration in the injury response are still unknown, and consequently, therapeutic approaches and treatment results are diverse. To exploit the full potential of this burgeoning field of medicine, further research will be required and is ongoing. This review summarises the current knowledge of commonly used regenerative medicine treatments in equine patients and critically discusses their use.
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Filipczak, Nina, Satya Siva Kishan Yalamarty, Xiang Li, Muhammad Muzamil Khan, Farzana Parveen, and Vladimir Torchilin. "Lipid-Based Drug Delivery Systems in Regenerative Medicine." Materials 14, no. 18 (September 17, 2021): 5371. http://dx.doi.org/10.3390/ma14185371.

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The most important goal of regenerative medicine is to repair, restore, and regenerate tissues and organs that have been damaged as a result of an injury, congenital defect or disease, as well as reversing the aging process of the body by utilizing its natural healing potential. Regenerative medicine utilizes products of cell therapy, as well as biomedical or tissue engineering, and is a huge field for development. In regenerative medicine, stem cells and growth factor are mainly used; thus, innovative drug delivery technologies are being studied for improved delivery. Drug delivery systems offer the protection of therapeutic proteins and peptides against proteolytic degradation where controlled delivery is achievable. Similarly, the delivery systems in combination with stem cells offer improvement of cell survival, differentiation, and engraftment. The present review summarizes the significance of biomaterials in tissue engineering and the importance of colloidal drug delivery systems in providing cells with a local environment that enables them to proliferate and differentiate efficiently, resulting in successful tissue regeneration.
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6

Yahya, Esam Bashir, A. A. Amirul, Abdul Khalil H.P.S., Niyi Gideon Olaiya, Muhammad Omer Iqbal, Fauziah Jummaat, Atty Sofea A.K., and A. S. Adnan. "Insights into the Role of Biopolymer Aerogel Scaffolds in Tissue Engineering and Regenerative Medicine." Polymers 13, no. 10 (May 17, 2021): 1612. http://dx.doi.org/10.3390/polym13101612.

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The global transplantation market size was valued at USD 8.4 billion in 2020 and is expected to grow at a compound annual growth rate of 11.5% over the forecast period. The increasing demand for tissue transplantation has inspired researchers to find alternative approaches for making artificial tissues and organs function. The unique physicochemical and biological properties of biopolymers and the attractive structural characteristics of aerogels such as extremely high porosity, ultra low-density, and high surface area make combining these materials of great interest in tissue scaffolding and regenerative medicine applications. Numerous biopolymer aerogel scaffolds have been used to regenerate skin, cartilage, bone, and even heart valves and blood vessels by growing desired cells together with the growth factor in tissue engineering scaffolds. This review focuses on the principle of tissue engineering and regenerative medicine and the role of biopolymer aerogel scaffolds in this field, going through the properties and the desirable characteristics of biopolymers and biopolymer tissue scaffolds in tissue engineering applications. The recent advances of using biopolymer aerogel scaffolds in the regeneration of skin, cartilage, bone, and heart valves are also discussed in the present review. Finally, we highlight the main challenges of biopolymer-based scaffolds and the prospects of using these materials in regenerative medicine.
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7

TABATA, Yasuhiko. "TISSUE ENGINEERING FOR REGENERATIVE MEDICINE." Japanese jornal of Head and Neck Cancer 28, no. 3 (2002): 573–79. http://dx.doi.org/10.5981/jjhnc1974.28.573.

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8

Różalska, Barbara, Bartłomiej Micota, Małgorzata Paszkiewicz, and Beata Sadowska. "Tissue engineering in regenerative medicine." Forum Zakażeń 6, no. 5 (November 2, 2015): 291–98. http://dx.doi.org/10.15374/fz2015052.

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9

Jenkins, D. Denison, George P. Yang, H. Peter Lorenz, Michael T. Longaker, and Karl G. Sylvester. "Tissue engineering and regenerative medicine." Clinics in Plastic Surgery 30, no. 4 (October 2003): 581–88. http://dx.doi.org/10.1016/s0094-1298(03)00076-2.

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10

Mao, Angelo S., and David J. Mooney. "Regenerative medicine: Current therapies and future directions." Proceedings of the National Academy of Sciences 112, no. 47 (November 24, 2015): 14452–59. http://dx.doi.org/10.1073/pnas.1508520112.

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Organ and tissue loss through disease and injury motivate the development of therapies that can regenerate tissues and decrease reliance on transplantations. Regenerative medicine, an interdisciplinary field that applies engineering and life science principles to promote regeneration, can potentially restore diseased and injured tissues and whole organs. Since the inception of the field several decades ago, a number of regenerative medicine therapies, including those designed for wound healing and orthopedics applications, have received Food and Drug Administration (FDA) approval and are now commercially available. These therapies and other regenerative medicine approaches currently being studied in preclinical and clinical settings will be covered in this review. Specifically, developments in fabricating sophisticated grafts and tissue mimics and technologies for integrating grafts with host vasculature will be discussed. Enhancing the intrinsic regenerative capacity of the host by altering its environment, whether with cell injections or immune modulation, will be addressed, as well as methods for exploiting recently developed cell sources. Finally, we propose directions for current and future regenerative medicine therapies.
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11

Rodríguez-Vázquez, Martin, Brenda Vega-Ruiz, Rodrigo Ramos-Zúñiga, Daniel Alexander Saldaña-Koppel, and Luis Fernando Quiñones-Olvera. "Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine." BioMed Research International 2015 (2015): 1–15. http://dx.doi.org/10.1155/2015/821279.

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Tissue engineering is an important therapeutic strategy to be used in regenerative medicine in the present and in the future. Functional biomaterials research is focused on the development and improvement of scaffolding, which can be used to repair or regenerate an organ or tissue. Scaffolds are one of the crucial factors for tissue engineering. Scaffolds consisting of natural polymers have recently been developed more quickly and have gained more popularity. These include chitosan, a copolymer derived from the alkaline deacetylation of chitin. Expectations for use of these scaffolds are increasing as the knowledge regarding their chemical and biological properties expands, and new biomedical applications are investigated. Due to their different biological properties such as being biocompatible, biodegradable, and bioactive, they have given the pattern for use in tissue engineering for repair and/or regeneration of different tissues including skin, bone, cartilage, nerves, liver, and muscle. In this review, we focus on the intrinsic properties offered by chitosan and its use in tissue engineering, considering it as a promising alternative for regenerative medicine as a bioactive polymer.
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12

McInnes, Adam D., Michael A. J. Moser, and Xiongbiao Chen. "Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering." Journal of Functional Biomaterials 13, no. 4 (November 14, 2022): 240. http://dx.doi.org/10.3390/jfb13040240.

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The multidisciplinary fields of tissue engineering and regenerative medicine have the potential to revolutionize the practise of medicine through the abilities to repair, regenerate, or replace tissues and organs with functional engineered constructs. To this end, tissue engineering combines scaffolding materials with cells and biologically active molecules into constructs with the appropriate structures and properties for tissue/organ regeneration, where scaffolding materials and biomolecules are the keys to mimic the native extracellular matrix (ECM). For this, one emerging way is to decellularize the native ECM into the materials suitable for, directly or in combination with other materials, creating functional constructs. Over the past decade, decellularized ECM (or dECM) has greatly facilitated the advance of tissue engineering and regenerative medicine, while being challenged in many ways. This article reviews the recent development of dECM for tissue engineering and regenerative medicine, with a focus on the preparation of dECM along with its influence on cell culture, the modification of dECM for use as a scaffolding material, and the novel techniques and emerging trends in processing dECM into functional constructs. We highlight the success of dECM and constructs in the in vitro, in vivo, and clinical applications and further identify the key issues and challenges involved, along with a discussion of future research directions.
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13

Saini, Gia, Nicole Segaran, Joseph L. Mayer, Aman Saini, Hassan Albadawi, and Rahmi Oklu. "Applications of 3D Bioprinting in Tissue Engineering and Regenerative Medicine." Journal of Clinical Medicine 10, no. 21 (October 26, 2021): 4966. http://dx.doi.org/10.3390/jcm10214966.

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Regenerative medicine is an emerging field that centers on the restoration and regeneration of functional components of damaged tissue. Tissue engineering is an application of regenerative medicine and seeks to create functional tissue components and whole organs. Using 3D printing technologies, native tissue mimics can be created utilizing biomaterials and living cells. Recently, regenerative medicine has begun to employ 3D bioprinting methods to create highly specialized tissue models to improve upon conventional tissue engineering methods. Here, we review the use of 3D bioprinting in the advancement of tissue engineering by describing the process of 3D bioprinting and its advantages over other tissue engineering methods. Materials and techniques in bioprinting are also reviewed, in addition to future clinical applications, challenges, and future directions of the field.
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14

Varga, Rita. "Historical background of regenerative medicine and tissue engineering." Kaleidoscope history 11, no. 22 (2021): 73–80. http://dx.doi.org/10.17107/kh.2021.22.73-80.

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The ancient desires of men there were emerging from time to time in the tales of many nations and are emerging actually in the screenplays of the film industry. Flying, travelling in space, visiting other planets, achieving eternal youth, becoming invulnerable or even the desire for quick recovery are deeply rooted in men’s fantasies and some of them are turning out step-by-step as a day-to-day reality. Regenerative medicine and tissue engineering are interdisciplinary fields of research that utilize the knowledge of engineers, scientists, and physicians to create tissue-like implants. In the most intensive research on tissue regeneration, there are taken cell samples of the patients’ relevant tissues, which after multiplication on a host artificial matrix are finally replaced to the damaged area for local regeneration. Henceforward, the regenerated tissue regains its original structure and function. The past four decades witnessed the rapid development of these fields, from laboratory experiments throughs animal testing and clinical trials to the administered therapies. Studying the history of original and novel ideas in this field is a key issue in understanding the latest achievements while appreciating the actual results and the future trends respectively. This study outlines a brief summary of the background of 7373he early history and the present challenges of regenerative medicine. In this study, I present a brief survey on the background of regenerative medicine and the principles of tissue engineering, followed by discussing the early years of these fields. In the end, I will describe the most relevant questions and scientific challenges that are still to be answered and overcome.
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15

Wu, David T., Jose G. Munguia-Lopez, Ye Won Cho, Xiaolu Ma, Vivian Song, Zhiyue Zhu, and Simon D. Tran. "Polymeric Scaffolds for Dental, Oral, and Craniofacial Regenerative Medicine." Molecules 26, no. 22 (November 22, 2021): 7043. http://dx.doi.org/10.3390/molecules26227043.

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Dental, oral, and craniofacial (DOC) regenerative medicine aims to repair or regenerate DOC tissues including teeth, dental pulp, periodontal tissues, salivary gland, temporomandibular joint (TMJ), hard (bone, cartilage), and soft (muscle, nerve, skin) tissues of the craniofacial complex. Polymeric materials have a broad range of applications in biomedical engineering and regenerative medicine functioning as tissue engineering scaffolds, carriers for cell-based therapies, and biomedical devices for delivery of drugs and biologics. The focus of this review is to discuss the properties and clinical indications of polymeric scaffold materials and extracellular matrix technologies for DOC regenerative medicine. More specifically, this review outlines the key properties, advantages and drawbacks of natural polymers including alginate, cellulose, chitosan, silk, collagen, gelatin, fibrin, laminin, decellularized extracellular matrix, and hyaluronic acid, as well as synthetic polymers including polylactic acid (PLA), polyglycolic acid (PGA), polycaprolactone (PCL), poly (ethylene glycol) (PEG), and Zwitterionic polymers. This review highlights key clinical applications of polymeric scaffolding materials to repair and/or regenerate various DOC tissues. Particularly, polymeric materials used in clinical procedures are discussed including alveolar ridge preservation, vertical and horizontal ridge augmentation, maxillary sinus augmentation, TMJ reconstruction, periodontal regeneration, periodontal/peri-implant plastic surgery, regenerative endodontics. In addition, polymeric scaffolds application in whole tooth and salivary gland regeneration are discussed.
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Suh, Hwal. "Tissue restoration, tissue engineering and regenerative medicine." Yonsei Medical Journal 41, no. 6 (2000): 681. http://dx.doi.org/10.3349/ymj.2000.41.6.681.

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17

Monteiro, Nelson, Albino Martins, Rui L. Reis, and Nuno M. Neves. "Liposomes in tissue engineering and regenerative medicine." Journal of The Royal Society Interface 11, no. 101 (December 6, 2014): 20140459. http://dx.doi.org/10.1098/rsif.2014.0459.

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Liposomes are vesicular structures made of lipids that are formed in aqueous solutions. Structurally, they resemble the lipid membrane of living cells. Therefore, they have been widely investigated, since the 1960s, as models to study the cell membrane, and as carriers for protection and/or delivery of bioactive agents. They have been used in different areas of research including vaccines, imaging, applications in cosmetics and tissue engineering. Tissue engineering is defined as a strategy for promoting the regeneration of tissues for the human body. This strategy may involve the coordinated application of defined cell types with structured biomaterial scaffolds to produce living structures. To create a new tissue, based on this strategy, a controlled stimulation of cultured cells is needed, through a systematic combination of bioactive agents and mechanical signals. In this review, we highlight the potential role of liposomes as a platform for the sustained and local delivery of bioactive agents for tissue engineering and regenerative medicine approaches.
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18

James, Roshan, and Cato T. Laurencin. "Musculoskeletal Regenerative Engineering: Biomaterials, Structures, and Small Molecules." Advances in Biomaterials 2014 (June 24, 2014): 1–12. http://dx.doi.org/10.1155/2014/123070.

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Musculoskeletal tissues are critical to the normal functioning of an individual and following damage or degeneration they show extremely limited endogenous regenerative capacity. The future of regenerative medicine is the combination of advanced biomaterials, structures, and cues to re-engineer/guide stem cells to yield the desired organ cells and tissues. Tissue engineering strategies were ideally suited to repair damaged tissues; however, the substitution and regeneration of large tissue volumes and multi-level tissues such as complex organ systems integrated into a single phase require more than optimal combinations of biomaterials and biologics. We highlight bioinspired advancements leading to novel regenerative scaffolds especially for musculoskeletal tissue repair and regeneration. Tissue and organ regeneration relies on the spatial and temporal control of biophysical and biochemical cues, including soluble molecules, cell-cell contacts, cell-extracellular matrix contacts, and physical forces. Strategies that recapitulate the complexity of the local microenvironment of the tissue and the stem cell niche play a crucial role in regulating cell self-renewal and differentiation. Biomaterials and scaffolds based on biomimicry of the native tissue will enable convergence of the advances in materials science, the advances in stem cell science, and our understanding of developmental biology.
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19

Campanacci, Domenico Andrea, Gianluca Scalici, and Maurizio Scorianz. "Regenerative medicine in orthopaedic surgery." International Journal of Bone Fragility 1, no. 3 (November 20, 2021): 107–13. http://dx.doi.org/10.57582/ijbf.210103.107.

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Regenerative medicine includes the use of technologies aimed at repairing or replacing damaged cells, tissues and organs, in order to restore their structure and function. The clinical indications for the use of regenerative medicine in orthopaedic surgery are degenerative diseases (arthritis, aseptic necrosis, osteochondritis), posttraumatic conditions (non-union) and osteoarticular segmental bone loss. The objective of tissue regeneration in orthopaedic surgery can be achieved with minimally invasive techniques or using open surgery with the application of biological or synthetic scaffolds, autologous mesenchymal stem cells, growth factors or specific surgical techniques and new-generation surgical devices. Three-dimensional bioprinting, the new frontier of tissue engineering, is a promising technology for regenerative medicine in orthopaedic surgery. In the present review, all the different techniques of bone tissue regeneration will be described with the aim of highlighting their evidence-based effectiveness and trying to define their specific role in different indications.
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20

Pina, Sandra, Viviana P. Ribeiro, Catarina F. Marques, F. Raquel Maia, Tiago H. Silva, Rui L. Reis, and J. Miguel Oliveira. "Scaffolding Strategies for Tissue Engineering and Regenerative Medicine Applications." Materials 12, no. 11 (June 5, 2019): 1824. http://dx.doi.org/10.3390/ma12111824.

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During the past two decades, tissue engineering and the regenerative medicine field have invested in the regeneration and reconstruction of pathologically altered tissues, such as cartilage, bone, skin, heart valves, nerves and tendons, and many others. The 3D structured scaffolds and hydrogels alone or combined with bioactive molecules or genes and cells are able to guide the development of functional engineered tissues, and provide mechanical support during in vivo implantation. Naturally derived and synthetic polymers, bioresorbable inorganic materials, and respective hybrids, and decellularized tissue have been considered as scaffolding biomaterials, owing to their boosted structural, mechanical, and biological properties. A diversity of biomaterials, current treatment strategies, and emergent technologies used for 3D scaffolds and hydrogel processing, and the tissue-specific considerations for scaffolding for Tissue engineering (TE) purposes are herein highlighted and discussed in depth. The newest procedures focusing on the 3D behavior and multi-cellular interactions of native tissues for further use for in vitro model processing are also outlined. Completed and ongoing preclinical research trials for TE applications using scaffolds and hydrogels, challenges, and future prospects of research in the regenerative medicine field are also presented.
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Mantha, Somasundar, Sangeeth Pillai, Parisa Khayambashi, Akshaya Upadhyay, Yuli Zhang, Owen Tao, Hieu M. Pham, and Simon D. Tran. "Smart Hydrogels in Tissue Engineering and Regenerative Medicine." Materials 12, no. 20 (October 12, 2019): 3323. http://dx.doi.org/10.3390/ma12203323.

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The field of regenerative medicine has tremendous potential for improved treatment outcomes and has been stimulated by advances made in bioengineering over the last few decades. The strategies of engineering tissues and assembling functional constructs that are capable of restoring, retaining, and revitalizing lost tissues and organs have impacted the whole spectrum of medicine and health care. Techniques to combine biomimetic materials, cells, and bioactive molecules play a decisive role in promoting the regeneration of damaged tissues or as therapeutic systems. Hydrogels have been used as one of the most common tissue engineering scaffolds over the past two decades due to their ability to maintain a distinct 3D structure, to provide mechanical support for the cells in the engineered tissues, and to simulate the native extracellular matrix. The high water content of hydrogels can provide an ideal environment for cell survival, and structure which mimics the native tissues. Hydrogel systems have been serving as a supportive matrix for cell immobilization and growth factor delivery. This review outlines a brief description of the properties, structure, synthesis and fabrication methods, applications, and future perspectives of smart hydrogels in tissue engineering.
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Hosseinkhani, Hossein, Abraham J. Domb, Ghorbanali Sharifzadeh, and Victoria Nahum. "Gene Therapy for Regenerative Medicine." Pharmaceutics 15, no. 3 (March 6, 2023): 856. http://dx.doi.org/10.3390/pharmaceutics15030856.

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The development of biological methods over the past decade has stimulated great interest in the possibility to regenerate human tissues. Advances in stem cell research, gene therapy, and tissue engineering have accelerated the technology in tissue and organ regeneration. However, despite significant progress in this area, there are still several technical issues that must be addressed, especially in the clinical use of gene therapy. The aims of gene therapy include utilising cells to produce a suitable protein, silencing over-producing proteins, and genetically modifying and repairing cell functions that may affect disease conditions. While most current gene therapy clinical trials are based on cell- and viral-mediated approaches, non-viral gene transfection agents are emerging as potentially safe and effective in the treatment of a wide variety of genetic and acquired diseases. Gene therapy based on viral vectors may induce pathogenicity and immunogenicity. Therefore, significant efforts are being invested in non-viral vectors to enhance their efficiency to a level comparable to the viral vector. Non-viral technologies consist of plasmid-based expression systems containing a gene encoding, a therapeutic protein, and synthetic gene delivery systems. One possible approach to enhance non-viral vector ability or to be an alternative to viral vectors would be to use tissue engineering technology for regenerative medicine therapy. This review provides a critical view of gene therapy with a major focus on the development of regenerative medicine technologies to control the in vivo location and function of administered genes.
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23

Terheyden, Hendrik K. W. "Future: Tissue Engineering (Regenerative Medicine); Mandible." Journal of Oral and Maxillofacial Surgery 67, no. 9 (September 2009): 11. http://dx.doi.org/10.1016/j.joms.2009.05.325.

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24

Hunt, John A., Rui Chen, Theun van Veen, and Nicholas Bryan. "Hydrogels for tissue engineering and regenerative medicine." J. Mater. Chem. B 2, no. 33 (2014): 5319–38. http://dx.doi.org/10.1039/c4tb00775a.

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Injectable hydrogels have become an incredibly prolific area of research in the field of tissue engineering and regenerative medicine, because of their high water content, mechanical similarity to natural tissues, and ease of surgical implantation, hydrogels are at the forefront of biomedical scaffold and drug carrier design.
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Friedrich, Ralf P., Iwona Cicha, and Christoph Alexiou. "Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering." Nanomaterials 11, no. 9 (September 8, 2021): 2337. http://dx.doi.org/10.3390/nano11092337.

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In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.
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Shabbirahmed, Asma Musfira, Rajkumar Sekar, Levin Anbu Gomez, Medidi Raja Sekhar, Samson Prince Hiruthyaswamy, Nagaraj Basavegowda, and Prathap Somu. "Recent Developments of Silk-Based Scaffolds for Tissue Engineering and Regenerative Medicine Applications: A Special Focus on the Advancement of 3D Printing." Biomimetics 8, no. 1 (January 2, 2023): 16. http://dx.doi.org/10.3390/biomimetics8010016.

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Regenerative medicine has received potential attention around the globe, with improving cell performances, one of the necessary ideas for the advancements of regenerative medicine. It is crucial to enhance cell performances in the physiological system for drug release studies because the variation in cell environments between in vitro and in vivo develops a loop in drug estimation. On the other hand, tissue engineering is a potential path to integrate cells with scaffold biomaterials and produce growth factors to regenerate organs. Scaffold biomaterials are a prototype for tissue production and perform vital functions in tissue engineering. Silk fibroin is a natural fibrous polymer with significant usage in regenerative medicine because of the growing interest in leftovers for silk biomaterials in tissue engineering. Among various natural biopolymer-based biomaterials, silk fibroin-based biomaterials have attracted significant attention due to their outstanding mechanical properties, biocompatibility, hemocompatibility, and biodegradability for regenerative medicine and scaffold applications. This review article focused on highlighting the recent advancements of 3D printing in silk fibroin scaffold technologies for regenerative medicine and tissue engineering.
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Häneke, Timm, and Makoto Sahara. "Progress in Bioengineering Strategies for Heart Regenerative Medicine." International Journal of Molecular Sciences 23, no. 7 (March 23, 2022): 3482. http://dx.doi.org/10.3390/ijms23073482.

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The human heart has the least regenerative capabilities among tissues and organs, and heart disease continues to be a leading cause of mortality in the industrialized world with insufficient therapeutic options and poor prognosis. Therefore, developing new therapeutic strategies for heart regeneration is a major goal in modern cardiac biology and medicine. Recent advances in stem cell biology and biotechnologies such as human pluripotent stem cells (hPSCs) and cardiac tissue engineering hold great promise for opening novel paths to heart regeneration and repair for heart disease, although these areas are still in their infancy. In this review, we summarize and discuss the recent progress in cardiac tissue engineering strategies, highlighting stem cell engineering and cardiomyocyte maturation, development of novel functional biomaterials and biofabrication tools, and their therapeutic applications involving drug discovery, disease modeling, and regenerative medicine for heart disease.
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Mahla, Ranjeet Singh. "Stem Cells Applications in Regenerative Medicine and Disease Therapeutics." International Journal of Cell Biology 2016 (2016): 1–24. http://dx.doi.org/10.1155/2016/6940283.

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Regenerative medicine, the most recent and emerging branch of medical science, deals with functional restoration of tissues or organs for the patient suffering from severe injuries or chronic disease. The spectacular progress in the field of stem cell research has laid the foundation for cell based therapies of disease which cannot be cured by conventional medicines. The indefinite self-renewal and potential to differentiate into other types of cells represent stem cells as frontiers of regenerative medicine. The transdifferentiating potential of stem cells varies with source and according to that regenerative applications also change. Advancements in gene editing and tissue engineering technology have endorsed the ex vivo remodelling of stem cells grown into 3D organoids and tissue structures for personalized applications. This review outlines the most recent advancement in transplantation and tissue engineering technologies of ESCs, TSPSCs, MSCs, UCSCs, BMSCs, and iPSCs in regenerative medicine. Additionally, this review also discusses stem cells regenerative application in wildlife conservation.
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Heydari, Zahra, Mustapha Najimi, Hamed Mirzaei, Anastasia Shpichka, Marc Ruoss, Zahra Farzaneh, Leila Montazeri, et al. "Tissue Engineering in Liver Regenerative Medicine: Insights into Novel Translational Technologies." Cells 9, no. 2 (January 27, 2020): 304. http://dx.doi.org/10.3390/cells9020304.

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Organ and tissue shortage are known as a crucially important public health problem as unfortunately a small percentage of patients receive transplants. In the context of emerging regenerative medicine, researchers are trying to regenerate and replace different organs and tissues such as the liver, heart, skin, and kidney. Liver tissue engineering (TE) enables us to reproduce and restore liver functions, fully or partially, which could be used in the treatment of acute or chronic liver disorders and/or generate an appropriate functional organ which can be transplanted or employed as an extracorporeal device. In this regard, a variety of techniques (e.g., fabrication technologies, cell-based technologies, microfluidic systems and, extracorporeal liver devices) could be applied in tissue engineering in liver regenerative medicine. Common TE techniques are based on allocating stem cell-derived hepatocyte-like cells or primary hepatocytes within a three-dimensional structure which leads to the improvement of their survival rate and functional phenotype. Taken together, new findings indicated that developing liver tissue engineering-based techniques could pave the way for better treatment of liver-related disorders. Herein, we summarized novel technologies used in liver regenerative medicine and their future applications in clinical settings.
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Järvinen, Tero A. H., and Toini Pemmari. "Systemically Administered, Target-Specific, Multi-Functional Therapeutic Recombinant Proteins in Regenerative Medicine." Nanomaterials 10, no. 2 (January 28, 2020): 226. http://dx.doi.org/10.3390/nano10020226.

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Growth factors, chemokines and cytokines guide tissue regeneration after injuries. However, their applications as recombinant proteins are almost non-existent due to the difficulty of maintaining their bioactivity in the protease-rich milieu of injured tissues in humans. Safety concerns have ruled out their systemic administration. The vascular system provides a natural platform for circumvent the limitations of the local delivery of protein-based therapeutics. Tissue selectivity in drug accumulation can be obtained as organ-specific molecular signatures exist in the blood vessels in each tissue, essentially forming a postal code system (“vascular zip codes”) within the vasculature. These target-specific “vascular zip codes” can be exploited in regenerative medicine as the angiogenic blood vessels in the regenerating tissues have a unique molecular signature. The identification of vascular homing peptides capable of finding these unique “vascular zip codes” after their systemic administration provides an appealing opportunity for the target-specific delivery of therapeutics to tissue injuries. Therapeutic proteins can be “packaged” together with homing peptides by expressing them as multi-functional recombinant proteins. These multi-functional recombinant proteins provide an example how molecular engineering gives to a compound an ability to home to regenerating tissue and enhance its therapeutic potential. Regenerative medicine has been dominated by the locally applied therapeutic approaches despite these therapies are not moving to clinical medicine with success. There might be a time to change the paradigm towards systemically administered, target organ-specific therapeutic molecules in future drug discovery and development for regenerative medicine.
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Veeman, Dhinakaran, M. Swapna Sai, P. Sureshkumar, T. Jagadeesha, L. Natrayan, M. Ravichandran, and Wubishet Degife Mammo. "Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends." International Journal of Polymer Science 2021 (September 8, 2021): 1–20. http://dx.doi.org/10.1155/2021/4907027.

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As a technique of producing fabric engineering scaffolds, three-dimensional (3D) printing has tremendous possibilities. 3D printing applications are restricted to a wide range of biomaterials in the field of regenerative medicine and tissue engineering. Due to their biocompatibility, bioactiveness, and biodegradability, biopolymers such as collagen, alginate, silk fibroin, chitosan, alginate, cellulose, and starch are used in a variety of fields, including the food, biomedical, regeneration, agriculture, packaging, and pharmaceutical industries. The benefits of producing 3D-printed scaffolds are many, including the capacity to produce complicated geometries, porosity, and multicell coculture and to take growth factors into account. In particular, the additional production of biopolymers offers new options to produce 3D structures and materials with specialised patterns and properties. In the realm of tissue engineering and regenerative medicine (TERM), important progress has been accomplished; now, several state-of-the-art techniques are used to produce porous scaffolds for organ or tissue regeneration to be suited for tissue technology. Natural biopolymeric materials are often better suited for designing and manufacturing healing equipment than temporary implants and tissue regeneration materials owing to its appropriate properties and biocompatibility. The review focuses on the additive manufacturing of biopolymers with significant changes, advancements, trends, and developments in regenerative medicine and tissue engineering with potential applications.
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Phutane, Prasanna, Darshan Telange, Surendra Agrawal, Mahendra Gunde, Kunal Kotkar, and Anil Pethe. "Biofunctionalization and Applications of Polymeric Nanofibers in Tissue Engineering and Regenerative Medicine." Polymers 15, no. 5 (February 27, 2023): 1202. http://dx.doi.org/10.3390/polym15051202.

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The limited ability of most human tissues to regenerate has necessitated the interventions namely autograft and allograft, both of which carry the limitations of its own. An alternative to such interventions could be the capability to regenerate the tissue in vivo.Regeneration of tissue using the innate capacity of the cells to regenerate is studied under the discipline of tissue engineering and regenerative medicine (TERM). Besides the cells and growth-controlling bioactives, scaffolds play the central role in TERM which is analogous to the role performed by extracellular matrix (ECM) in the vivo. Mimicking the structure of ECM at the nanoscale is one of the critical attributes demonstrated by nanofibers. This unique feature and its customizable structure to befit different types of tissues make nanofibers a competent candidate for tissue engineering. This review discusses broad range of natural and synthetic biodegradable polymers employed to construct nanofibers as well as biofunctionalization of polymers to improve cellular interaction and tissue integration. Amongst the diverse ways to fabricate nanofibers, electrospinning has been discussed in detail along with advances in this technique. Review also presents a discourse on application of nanofibers for a range of tissues, namely neural, vascular, cartilage, bone, dermal and cardiac.
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Pinto, F., A. Calarco, A. Brescia, E. Sacco, A. D'addessi, M. Racioppi, and P. F. Bassi. "Regenerative Medicine: Applications and Development in Urology." Urologia Journal 74, no. 4 (October 2007): 197–205. http://dx.doi.org/10.1177/039156030707400402.

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Purpose Congenital abnormalities and acquired disorders can lead to organ damage and loss. Nowadays, transplantation represents the only effective treatment option. However, there is a marked decrease in the number of organ donors, which is even yearly worsening due to the population aging. The regenerative medicine represents a realistic option that allows to restore and maintain the normal functions of tissues and organs. This article reviews the principles of regenerative medicine and the recent advances with regard to its application to the genitourinary tract. Recent findings The field of regenerative medicine involves different areas of technology, such as tissue engineering, stem cells and cloning. Tissue engineering involves the field of cell transplantation, materials science and engineering in order to create functional replacement tissues. Stem cells and cloning permit the extraction of pluripotent, embryonic stem cells offering a potentially limitless source of cells for tissue engineering applications. Most current strategies for tissue engineering depend upon a sample of autologous cells from the patient's diseased organ. Biopsies from patients with extensive end-stage organ failure, however, may not yield enough normal cells. In these situations, stem cells are envisaged as being an alternative source. Stem cells can be derived from discarded human embryos (human embryonic stem cells), from fetal tissue or from adult sources (bone marrow, fat, skin). Therapeutic cloning offers a potentially limitless source of cells for tissue engineering applications. Regenerative medicine and tissue engineering scientists have increasingly applied the principles of cell transplantation, materials science and bioengineering to construct biological substitutes that will restore and maintain normal function in urological diseased and injured tissues such as kidney, ureter, bladder, urethra and penis. Conclusions Regenerative medicine offers several applications in acquired and congenital genitourinary diseases. Tissue engineering, stem cells and, mostly, cloning have been applied in experimental studies with excellent results. Few preliminary human applications have been developed with promising results.
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Wardhana, Aditya, and Michelle Valeria. "Tissue Engineering and Regenerative Medicine: A Review." Jurnal Plastik Rekonstruksi 7, no. 1 (April 25, 2020): 10–17. http://dx.doi.org/10.14228/jpr.v7i1.278.

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Summary: The concept of tissue engineering has now been accounted for one of the most prospective answers to the growing needs of tissue and organ replacements. Many studies and researches are continuously done to achieve a paramount strategy in tissue engineering and regenerative medicine. This review emphasizes the concept, strategies, current application, and current challenges in tissue engineering. The strategy in tissue engineering has much improved and successfully applied in several reconstructive cases. Understanding of isolated cells’ behaviors, materials suitable for its’ scaffolds, in adjuncts with specific growth-inducing factors for each specific tissue or organ built is the key for successful tissue engineering. Ringkasan: Konsep tissue engineering merupakan salah satu jawaban yang paling diharapkan dapat memenuhi kebutuhan pengganti jaringan dan organ yang terus meningkat pada saat ini. Beragam studi dan penelitian secara terus-menerus dilakukan agar dapat memperoleh strategi terbaik dalam tissue engineering dan regenerative medicine. Artikel ini berfokus pada konsep, strategi, aplikasi terkini, dan tantangan di masa mendatang pada tissue engineering. Strategi tissue engineering telah banyak berkembangan dan berhasil diterapkan pada kasus-kasus rekonstruksi. Pemahaman mengenai perilaku sel, kecocokan material dengan scaffolds, serta faktor pendukung pertumbuhan untuk masing-masing jaringan atau organ spesifik yang akan diciptakan merupakan kunci keberhasian tissue engineering.
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Ghafoor, Robia. "Stem Cell Role in Regenerative Dental Medicine." Annals of Jinnah Sindh Medical University 8, no. 2 (December 30, 2022): 45–46. http://dx.doi.org/10.46663/ajsmu.v8i2.45-46.

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Regeneration therapies have widely permeated advanced research that aims to reproduce and repair a lost or damaged organ or tissue in order to restore the function and architecture as close to its original state as possible. Tissue engineering refers to the process of regeneration using techniques such as scaffold based cell cultures, stem cell therapy, and biomolecular signaling.
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Goker, Funda, Lena Larsson, Massimo Del Fabbro, and Farah Asa’ad. "Gene Delivery Therapeutics in the Treatment of Periodontitis and Peri-Implantitis: A State of the Art Review." International Journal of Molecular Sciences 20, no. 14 (July 20, 2019): 3551. http://dx.doi.org/10.3390/ijms20143551.

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Background: Periodontal disease is a chronic inflammatory condition that affects supporting tissues around teeth, resulting in periodontal tissue breakdown. If left untreated, periodontal disease could have serious consequences; this condition is in fact considered as the primary cause of tooth loss. Being highly prevalent among adults, periodontal disease treatment is receiving increased attention from researchers and clinicians. When this condition occurs around dental implants, the disease is termed peri-implantitis. Periodontal regeneration aims at restoring the destroyed attachment apparatus, in order to improve tooth stability and thus reduce disease progression and subsequent periodontal tissue breakdown. Although many biomaterials have been developed to promote periodontal regeneration, they still have their own set of disadvantages. As a result, regenerative medicine has been employed in the periodontal field, not only to overcome the drawbacks of the conventional biomaterials but also to ensure more predictable regenerative outcomes with minimal complications. Regenerative medicine is considered a part of the research field called tissue engineering/regenerative medicine (TE/RM), a translational field combining cell therapy, biomaterial, biomedical engineering and genetics all with the aim to replace and restore tissues or organs to their normal function using in vitro models for in vivo regeneration. In a tissue, cells are responding to different micro-environmental cues and signaling molecules, these biological factors influence cell differentiation, migration and cell responses. A central part of TE/RM therapy is introducing drugs, genetic materials or proteins to induce specific cellular responses in the cells at the site of tissue repair in order to enhance and improve tissue regeneration. In this review, we present the state of art of gene therapy in the applications of periodontal tissue and peri-implant regeneration. Purpose: We aim herein to review the currently available methods for gene therapy, which include the utilization of viral/non-viral vectors and how they might serve as therapeutic potentials in regenerative medicine for periodontal and peri-implant tissues.
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Barceló, Xavier, Stefan Scheurer, Rajesh Lakshmanan, Cathal J. Moran, Fiona Freeman, and Daniel J. Kelly. "3D bioprinting for meniscus tissue engineering: a review of key components, recent developments and future opportunities." Journal of 3D Printing in Medicine 5, no. 4 (December 2021): 213–33. http://dx.doi.org/10.2217/3dp-2021-0017.

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3D bioprinting has the potential to transform the field of regenerative medicine as it enables the precise spatial patterning of biomaterials, cells and biomolecules to produce engineered tissues. Although numerous tissue engineering strategies have been developed for meniscal repair, the field has yet to realize an implant capable of completely regenerating the tissue. This paper first summarized existing meniscal repair strategies, highlighting the importance of engineering biomimetic implants for successful meniscal regeneration. Next, we reviewed how developments in 3D (bio)printing are accelerating the engineering of functional meniscal tissues and the development of implants targeting damaged or diseased menisci. Some of the opportunities and challenges associated with use of 3D bioprinting for meniscal tissue engineering are identified. Finally, we discussed key emerging research areas with the capacity to enhance the bioprinting of meniscal grafts.
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Hao, Dake, Juan-Maria Lopez, Jianing Chen, Alexandra Maria Iavorovschi, Nora Marlene Lelivelt, and Aijun Wang. "Engineering Extracellular Microenvironment for Tissue Regeneration." Bioengineering 9, no. 5 (May 8, 2022): 202. http://dx.doi.org/10.3390/bioengineering9050202.

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The extracellular microenvironment is a highly dynamic network of biophysical and biochemical elements, which surrounds cells and transmits molecular signals. Extracellular microenvironment controls are of crucial importance for the ability to direct cell behavior and tissue regeneration. In this review, we focus on the different components of the extracellular microenvironment, such as extracellular matrix (ECM), extracellular vesicles (EVs) and growth factors (GFs), and introduce engineering approaches for these components, which can be used to achieve a higher degree of control over cellular activities and behaviors for tissue regeneration. Furthermore, we review the technologies established to engineer native-mimicking artificial components of the extracellular microenvironment for improved regenerative applications. This review presents a thorough analysis of the current research in extracellular microenvironment engineering and monitoring, which will facilitate the development of innovative tissue engineering strategies by utilizing different components of the extracellular microenvironment for regenerative medicine in the future.
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Tabata, Yasuhiko. "Positioning of Tissue Engineering in Regenerative Medicine." Inflammation and Regeneration 34, no. 1 (2014): 001–3. http://dx.doi.org/10.2492/inflammregen.34.001.

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Bono, Epifania, Stephanie H. Mathes, Nicola Franscini, and ursula Graf-Hausner. "Tissue Engineering – The Gateway to Regenerative Medicine." CHIMIA International Journal for Chemistry 64, no. 11 (November 26, 2010): 808–12. http://dx.doi.org/10.2533/chimia.2010.808.

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Stoltz, J. F., V. Decot, C. Huseltein, X. He, L. Zhang, J. Magdalou, Y. P. Li, et al. "Introduction to regenerative medicine and tissue engineering." Bio-Medical Materials and Engineering 22, no. 1-3 (2012): 3–16. http://dx.doi.org/10.3233/bme-2012-0684.

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Badylak, Stephen F., and Robert M. Nerem. "Progress in tissue engineering and regenerative medicine." Proceedings of the National Academy of Sciences 107, no. 8 (February 23, 2010): 3285–86. http://dx.doi.org/10.1073/pnas.1000256107.

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Sitharaman, Balaji. "Nanotechnology in Tissue Engineering and Regenerative Medicine." Tissue Engineering Part B: Reviews 18, no. 1 (February 2012): 76. http://dx.doi.org/10.1089/ten.teb.2011.1501.

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Sevastianov, V. I. "TECHNOLOGIES OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE." Russian Journal of Transplantology and Artificial Organs, no. 3 (September 24, 2014): 93. http://dx.doi.org/10.15825/1995-1191-2014-3-93-108.

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Williams, D. J., and I. M. Sebastine. "Tissue engineering and regenerative medicine: manufacturing challenges." IEE Proceedings - Nanobiotechnology 152, no. 6 (2005): 207. http://dx.doi.org/10.1049/ip-nbt:20050001.

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Atala, Anthony. "Regenerative Medicine and Tissue Engineering in Urology." Urologic Clinics of North America 36, no. 2 (May 2009): 199–209. http://dx.doi.org/10.1016/j.ucl.2009.02.009.

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Hellman, Kiki B., and Robert M. Nerem. "Editorial: Advancing Tissue Engineering and Regenerative Medicine." Tissue Engineering 13, no. 12 (December 2007): 2823–24. http://dx.doi.org/10.1089/ten.2007.1504.

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Headon, Denis R. "Workshop on Tissue Engineering/Regenerative Medicine: Houston." Tissue Engineering 10, no. 3-4 (March 2004): 321. http://dx.doi.org/10.1089/107632704323061672.

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Kim, Hong Nam, Alex Jiao, Nathaniel S. Hwang, Min Sung Kim, Do Hyun Kang, Deok-Ho Kim, and Kahp-Yang Suh. "Nanotopography-guided tissue engineering and regenerative medicine." Advanced Drug Delivery Reviews 65, no. 4 (April 2013): 536–58. http://dx.doi.org/10.1016/j.addr.2012.07.014.

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Chew, Sing Yian. "MicroRNAs in tissue engineering & regenerative medicine." Advanced Drug Delivery Reviews 88 (July 2015): 1–2. http://dx.doi.org/10.1016/j.addr.2015.07.001.

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