Relevant bibliographies by topics / Tissue engineering – laboratory manuals

Academic literature on the topic 'Tissue engineering – laboratory manuals'

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Published: 26 July 2024
Last updated: 26 July 2024

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Journal articles on the topic "Tissue engineering – laboratory manuals"

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Karthikeyan Ramalingam,. "Innovations in Oral Pathology Laboratory - A Mini Review." International Journal of Head and Neck Pathology 6, no. 2 (October 13, 2023): 1–5. http://dx.doi.org/10.56501/intjheadneckpathol.v6i1.914.

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Over the past two decades, a multitude of technological advancements have been integrated into histopathology laboratories, offering tools to enhance standardization and ensure occupational safety. Digital tracking plays a central role in guiding the entire process, from labeling cassettes and slides to the final stages of generating whole slide images, and storage of tissue blocks and tissue sections. Versatile equipment has effectively replaced time-consuming manual tasks, which were susceptible to errors and material loss. Currently, collaborative robots are assuming responsibilities once exclusively carried out by humans. The emergence of these novel technologies is anticipated to help in improving oral pathology laboratory practices.
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Iglesias, Carla, Lu Luo, Javier Martínez, Daniel J. Kelly, Javier Taboada, and Ignacio Pérez. "Obtaining the sGAG distribution profile in articular cartilage color images." Biomedical Engineering / Biomedizinische Technik 64, no. 5 (September 25, 2019): 591–600. http://dx.doi.org/10.1515/bmt-2018-0055.

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Abstract The articular cartilage tissue is an essential component of joints as it reduces the friction between the two bones. Its load-bearing properties depend mostly on proteoglycan distribution, which can be analyzed through the study of the presence of sulfated glycosaminoglycan (sGAG). Currently, sGAG distribution in articular cartilage is not completely known; it is calculated by means of laboratory tests that imply the inherent inaccuracy of a manual procedure. This paper presents an easy-to-use desktop software application for obtaining the sGAG distribution profile in tissue. This app uses color images of stained cartilage tissues taken under a microscope, so researchers at the Trinity Centre for Bioengineering (Dublin, Ireland) can understand the qualitative distribution of sGAG with depth in the studied tissues.
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Newman-Gage, Helen. "Application of Quality Assurance Practices in Processing Cells and Tissues for Transplantation." Cell Transplantation 4, no. 5 (September 1995): 447–54. http://dx.doi.org/10.1177/096368979500400506.

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Attention to issues of quality assurance from the early stages of development of experimental cell therapies provides a margin of safety for recipients. Adherence to minimum standards of practice at acquisition, processing, storage, and implantation ensures not only this baseline safety factor for patients, but also provides a baseline for comparative evaluations between different studies or different banks. This paper describes the basic components of a quality assurance program tailored to laboratories and facilities that collect, process, or distribute human cells and tissues for transplantation. These components include policy and procedure manuals, donor screening practices, processing procedures evaluation and control, training and education programs, auditing and investigation roles, responsibility for release of grafts, and recordkeeping and traceability requirements. References and resources for detailed information related to good manufacturing practices and good clinical and laboratory practices are provided. Standards, regulations, and current legislation specifically related to human cells and tissues intended for transplantation are also discussed.
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Speicher, Timothy E., Noelle M. Selkow, and Aric J. Warren. "Manual Therapy Improves Immediate Blood Flow and Tissue Fiber Alignment of the Forearm Extensors." Journal of Physical Medicine and Rehabilitation 4, no. 2 (August 24, 2022): 28–36. http://dx.doi.org/10.33696/rehabilitation.4.029.

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Background: Manual therapy is commonly used by clinicians to improve blood flow and tissue fiber orientation. Hypothesis/Purpose: Using diagnostic ultrasound, the purpose of the study was to examine how the application of Positional Release Therapy (PRT), instrumented assisted soft tissue mobilization (IASTM), therapeutic ultrasound (US) and a combination of all three, affect lateral elbow immediate blood flow and tissue fiber alignment. Study Design: Controlled laboratory study. Methods: Twenty-five participants (26.0 ± 4.5 years; 69.3 ± 4.3 cm; 81.8 ± 16.9 kg) received PRT =13, US =12, IASTM =13, and a combination treatment = 12. Results: Blood flow was significantly higher following PRT (691.54 ± 1237.16 mm2) compared to IASTM (18.73 ± 227.10 mm2) (p=0.050; ES=0.073 (0.16-1.5) and US (-10.09 ± 479.26 mm2) (p=0.042; ES=0.72 (-0.03-1.29), but no different from the combination intervention (627.64 ± 820.22 mm2) (p=0.849). Seventy-five percent of elbows in the PRT intervention showed improvement in blood flow, 54% in the IASTM group, 45% in US, and 73% in the combination group. Tissue fiber alignment was significantly better following IASTM (-5756.00 ± 8156.19 mm2) compared to PRT (-1552.54 ± 3896.58 mm2) (p=0.042; ES=0.66 (-0.01 – 1.31), but no difference was demonstrated among the other interventions (p>0.066). All elbows (100%) that received IASTM showed improved tissue orientation, 77% in the PRT group, 64% in US and 64% in the combination group. Conclusion: Manual therapy, particularly PRT and IASTM, seem to be better at increasing blood flow and muscle fiber orientation, respectively. Level of Evidence: II.
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Ferreira, Mónica V., Wilhelm Jahnen-Dechent, and Sabine Neuss. "Standardization of Automated Cell-Based Protocols for Toxicity Testing of Biomaterials." Journal of Biomolecular Screening 16, no. 6 (April 25, 2011): 647–54. http://dx.doi.org/10.1177/1087057111405380.

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Advances in high-throughput screening (HTS) instrumentation have led to enormous reduction of costs (e.g., of pipetting stations) and to the development of smaller instruments for automation of day-to-day routines in small research laboratories. In the biomaterials community, there has been an increasing interest for standardized screening protocols to identify cell type–specific cytocompatible biomaterials suitable for tissue engineering (TE) applications. In this study, the authors established a multiplexed assay protocol for toxicity screening of biomaterials using a low- to medium-throughput robotic liquid handling station (LHS). The protocol contains analysis of viability, cytotoxicity, and apoptosis combined in one assay. This study includes performance results of a side-by-side comparison of the EpMotion 5070 LHS and conventional pipetting/dispensing systems. Critical parameters were optimized each for a given platform. Higher accuracy and reproducibility were achieved for LHS compared to manually treated samples. The practicability and accuracy of the method in a typical small laboratory setting were tested by running daily routine tasks by trained and untrained laboratory staff. In addition, advantages and disadvantages as well as the step-by-step application protocol are reported. The approach described provides a potential utility in screening biomaterials toxicity, allowing researchers to meet the needs of low- and medium-throughput laboratories.
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Lee, H. P. "Comparison between Traditional and Web-Based Interactive Manuals for Laboratory-Based Subjects." International Journal of Mechanical Engineering Education 30, no. 4 (October 2002): 307–14. http://dx.doi.org/10.7227/ijmee.30.4.3.

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This paper presents the salient features of web-based interactive manuals for laboratory-based subjects and compares them with traditional laboratory manuals. The manuals utilize the latest developments in web applications, JAVA programming and pre-designed spreadsheets with the objective of reducing the time taken for students to perform the mundane and laborious calculations and plotting of graphs. The students can therefore utilize the time in the laboratories on the analysis and discussion of results besides performing the actual experiments. Moreover, the web-based interactive manuals incorporate virtual experiments, digital pictures, animated GIF files and quick movies to familiarize the students with the experimental set-up before performing the physical experiments. The proposed approach is applicable for laboratory-based subjects, which are not amenable to be converted completely to virtual laboratories.
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Chou, P. H., Y. L. Chou, H. C. Wei, C. S. Ho, and S. S. Jiang. "Biomechanical Analysis of Wrist Loading During Lifting Tasks." Journal of Mechanics 17, no. 4 (December 2001): 179–87. http://dx.doi.org/10.1017/s172771910000191x.

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ABSTRACTThis study develops a kinematic/kinetic model to evaluate the effect of different handle angles on wrist loading during lifting task. An imaged-based motion analysis system is used to study the movement pattern, force and moment of the wrist joint among nine different handle angles. Six CCD cameras were used to record 3-D trajectories of limb-mounted markers based on the laboratory coordination system as defined by an 8-marker cube. Euler angles are used to describe the orientation of a distal segment reference frame relative to a proximal segment reference frame. Each segment of the upper extremities is regarded as a uniform rigid body with six degrees of freedom. The resultant loading of the wrist joints was determined using an inverse dynamic procedure.This study indicates that tool handles can be designed or selected to reduce manual loading and the potential for injury during tool use. The mean curve of joint force and moment provided consultations and understandings of the wrist loading during lifting task. In this study, handles that kept the wrist joints in a dorsiflexed and radial deviated position, showed significant reduction in stresses around the surrounding soft tissue.
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Kelder, Cindy, Astrid Bakker, Jenneke Klein-Nulend, and Daniël Wismeijer. "The 3D Printing of Calcium Phosphate with K-Carrageenan under Conditions Permitting the Incorporation of Biological Components—A Method." Journal of Functional Biomaterials 9, no. 4 (October 17, 2018): 57. http://dx.doi.org/10.3390/jfb9040057.

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Critical-size bone defects are a common clinical problem. The golden standard to treat these defects is autologous bone grafting. Besides the limitations of availability and co-morbidity, autografts have to be manually adapted to fit in the defect, which might result in a sub-optimal fit and impaired healing. Scaffolds with precise dimensions can be created using 3-dimensional (3D) printing, enabling the production of patient-specific, ‘tailor-made’ bone substitutes with an exact fit. Calcium phosphate (CaP) is a popular material for bone tissue engineering due to its biocompatibility, osteoconductivity, and biodegradable properties. To enhance bone formation, a bioactive 3D-printed CaP scaffold can be created by combining the printed CaP scaffold with biological components such as growth factors and cytokines, e.g., vascular endothelial growth factor (VEGF), bone morphogenetic protein-2 (BMP-2), and interleukin-6 (IL-6). However, the 3D-printing of CaP with a biological component is challenging since production techniques often use high temperatures or aggressive chemicals, which hinders/inactivates the bioactivity of the incorporated biological components. Therefore, in our laboratory, we routinely perform extrusion-based 3D-printing with a biological binder at room temperature to create porous scaffolds for bone healing. In this method paper, we describe in detail a 3D-printing procedure for CaP paste with K-carrageenan as a biological binder.
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Kyzlasov, P. S., F. G. Kolpacynidi, D. V. Kazantsev, V. I. Doga, A. N. Bashkov, and O. V. Parinov. "NON-TRAUMATIC FORNIX RUPTURE WITH CONTRAST EXTRAVASATION." MEDICAL RADIOLOGY AND RADIATION SAFETY 67, no. 2 (April 2022): 73–75. http://dx.doi.org/10.33266/1024-6177-2022-67-2-73-75.

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Purpose: Description of a clinical case of surgical treatment of nontraumatic rupture of the renal calyx fornix, with extravasation of contrast agent into the perirenal tissue. Material and methods: Provided surgical treatment to a patient with spontaneous rupture of the calyx fornix on the background of ureteral obstruction caused by a stone. Results: Considering the concomitant pathology in the form of obstructive pyelonephritis, it was decided to drain the upper urinary tract with an external stent. After 7 days, the patient underwent replacement of the external stent with the internal one. The patient underwent antibacterial, anti-inflammatory therapy, with a positive clinical, laboratory effect. Retrograde pyelography was performed 7 days later. Violations of the integrity of the cavity system of the left kidney were not revealed. The external stent was replaced with an internal one. After 2 months, ureterolitho-extraction was performed on the left. Conclusion: From the data of the world literature and our own experience, we can conclude that spontaneous, non-traumatic rupture of the fornix is an extremely rare, urgent urological disease. We assume that the development of this process is similar to the Burhave syndrome, since smooth muscle fibers are present in the esophagus as well as in the esophagus. In order to provide immediate assistance to such patients, it is necessary to urgently drain the upper urinary tract. Timely diagnosis of this condition requires rapid tactics in the form of immediate ultrasound, radiological diagnostic methods, as well as computed tomography. The operational manual on the removal of stones in this case should be postponed for 2–3 weeks.
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Hj. Idrus, Ruszymah, and Aminuddin Saim. "Tissue Engineering Laboratory in Malaysia." Asia-Pacific Biotech News 09, no. 14 (July 30, 2005): 664–67. http://dx.doi.org/10.1142/s0219030305001886.

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Dissertations / Theses on the topic "Tissue engineering – laboratory manuals"

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Ghetti, Martina <1988&gt. "New Frontiers of Skin Tissue Engineering: from the Laboratory to Clinical Practice." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2017. http://amsdottorato.unibo.it/8061/1/PhD%20thesis%20Martina%20Ghetti.pdf.

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This thesis is focused on the key role of extracellular matrix in skin tissue engineering. Firstly, I presented three dimensional structures derived through the physiological secretion of extracellular matrix (ECM), that may be a bioinspired scaffold. I performed a biological characterization of cell-assembled ECMs from three different sub-populations of skin fibroblasts; papillary fibroblasts (Pfi), reticular fibroblasts (Rfi), and dermal papilla fibroblasts (DPfi). Fibroblast sub-populations were cultured with ascorbic acid to promote cell-assembled matrix production for 10 days. Cells were removed and the remaining matrices were characterized. I found that the ECM assembled by Pfi exhibited randomly oriented fibers, associated with highest interfibrillar space, reflecting ECM characteristics which are physiologically present within the papillary dermis. Mass spectrometry followed by immunofluorescence analysis showed that Thrombospondin is preferentially expressed within the DPfi ECM. In another experiment, keratinocytes were seeded on the top of cell depleted ECMs to generate epidermal skin constructs. I found that epidermal constructs grown on DPfi or Pfi matrices exhibited normal basement membrane formation, while Rfi matrices were unable to support membrane formation. Thus, inspiration should be taken from these different ECMs, to design therapeutic biomaterials in skin engineering applications. In the second part, I focused on human decellularized matrix for soft tissue repair and I investigated the biological interaction post-implant of this scaffold. The aims of this second part were to show the clinical results after the application of human decellularized matrix in patients suffering from abdominal hernia and to evaluate the response one year post implant, through morphological analysis of biopsy specimens. Clinical results showed that all the patients revealed a well tolerability of human decellularized matrix. Post-implant morphological results showed cellular repopulation, neo-angiogenesis, minimal inflammatory response and a well-organized collagen matrix in all biopsies. This scaffold can be considered a safe product to treat large abdominal defects.
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Alinejad, Mona. "The influence of modified pulp addition to market pulps on properties of laboratory tissue-grade handsheets with an example of Surrogate-based Kriging model and genetic algorithm for data analysis." Miami University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1500394831928467.

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Jalkanen, Ville. "Resonance sensor technology for detection of prostate cancer." Licentiate thesis, Umeå : Tillämpad fysik och elektronik, Umeå univ, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-896.

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Jelena, Vuletić Rakić. "Испитивање биокомпатибилности објеката од полимера произведених адитивном технологијом за примену у области стоматологије." Phd thesis, Univerzitet u Novom Sadu, Medicinski fakultet u Novom Sadu, 2016. http://www.cris.uns.ac.rs/record.jsf?recordId=101372&source=NDLTD&language=en.

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Uobičajeni pristup i testiranju biološkog ponašanja materijala je da se počne sa jednostavnim in vitro testovima baziranim na ćelijskim kulturama. In vitro testovi citotoksičnosti su danas jedan od osnovnih načina za procenu biološkog odgovora na materijal jer su brži, lakši za ponavljanje, ocenjivanje i jeftiniji u odnosu na eksperimente koji se izvode na životinjama i ljudima. Koriste se kao neka vrsta skrining testova za procenu biološke sigurnosti materijala. Za razliku od ćelijskih kultura, istraživanja koja uključuju eksperimentalne životinje pružaju bolji uvid u biokompatibilnost materijala, zbog mogućnosti praćenja kompleksnog imunološkog odgovora živog organizma. Smatraju se neophodnim za ocenu biloških odgovora na novi materijal, pre nego što se on upotrebi na ljudima. Mnogi aspekti biološkog odgovora ne mogu biti reprodukovani in vitro testovima uključujući krvne interakcije, zarastanje rana, reakcije preosetljivosti, karcinogenezu, hroničnu inflamaciju. Eksperimenti na životinjama pružaju informacije o ovim tipovima efekata bez izlaganja ljudi riziku. Cilj ovog istraživanja bio je da se oceni biokompatibilnost objekata od polimera na bazi epoksi smole Accura® ClearVue™ (hemijski sastav: 4,4’- izopropilidendicikloheksanol, produkti oligomerne reakcije sa 1-hlor-2,3- epoksipropanom(40-65%), smešа triaril-sulfonijum soli (50% propilen-karbonata i 50% triaril-sulfonijum heksafluoroantimonatnih soli) (1-10%) i 3-etil-3hidroksimetil-oksetan(10-20%). U oceni citotoksičnosti materijala Accura® ClearVue™ korišćeni su agar diguzioni i MTT test. Oba testa rađena sun a ćelijskim kulturama L929 (mišiji fibroblasti) i MRC-5 (humani fibroblasti). Ocena biokompatibilnosti testiranog materijala vršena je na osnovu urađenog testa iritacije oralne mukoze na modelu bukalne kesice hrčka, što je definisano standardom ISO 10993-10:2010. Biokompatibilnost materijala ispitana je i implantacijom uzoraka u potkožno tkivo dorzuma pacova soja Wistar.
Uobičajeni pristup i testiranju biološkog ponašanja materijala je da se počne sa jednostavnim in vitro testovima baziranim na ćelijskim kulturama. In vitro testovi citotoksičnosti su danas jedan od osnovnih načina za procenu biološkog odgovora na materijal jer su brži, lakši za ponavljanje, ocenjivanje i jeftiniji u odnosu na eksperimente koji se izvode na životinjama i ljudima. Koriste se kao neka vrsta skrining testova za procenu biološke sigurnosti materijala. Za razliku od ćelijskih kultura, istraživanja koja uključuju eksperimentalne životinje pružaju bolji uvid u biokompatibilnost materijala, zbog mogućnosti praćenja kompleksnog imunološkog odgovora živog organizma. Smatraju se neophodnim za ocenu biloških odgovora na novi materijal, pre nego što se on upotrebi na ljudima. Mnogi aspekti biološkog odgovora ne mogu biti reprodukovani in vitro testovima uključujući krvne interakcije, zarastanje rana, reakcije preosetljivosti, karcinogenezu, hroničnu inflamaciju. Eksperimenti na životinjama pružaju informacije o ovim tipovima efekata bez izlaganja ljudi riziku. Cilj ovog istraživanja bio je da se oceni biokompatibilnost objekata od polimera na bazi epoksi smole Accura® ClearVue™ (hemijski sastav: 4,4’- izopropilidendicikloheksanol, produkti oligomerne reakcije sa 1-hlor-2,3- epoksipropanom(40-65%), smeša triaril-sulfonijum soli (50% propilen-karbonata i 50% triaril-sulfonijum heksafluoroantimonatnih soli) (1-10%) i 3-etil-3hidroksimetil-oksetan(10-20%). U oceni citotoksičnosti materijala Accura® ClearVue™ korišćeni su agar diguzioni i MTT test. Oba testa rađena sun a ćelijskim kulturama L929 (mišiji fibroblasti) i MRC-5 (humani fibroblasti). Ocena biokompatibilnosti testiranog materijala vršena je na osnovu urađenog testa iritacije oralne mukoze na modelu bukalne kesice hrčka, što je definisano standardom ISO 10993-10:2010. Biokompatibilnost materijala ispitana je i implantacijom uzoraka u potkožno tkivo dorzuma pacova soja Wistar.
The usual approach in testing biological behavior of materials is to start with simple in vitro tests based on cell cultures. In vitro cytotoxicity tests are one of the basic methods of assessing the biological response to material because they are faster, cheaper, easier for repeating and evaluating compared to experiments carried out on animals and humans. They are used as a kind of screening test for evaluating the biosafety of materials. Unlike cell culture, studies involving experimental animals provide better insight into the biocompatibility of materials due to the possibility of monitoring the complex immune response of a living organism. They are considered necessary for assessing the biological response to new material before it is used on humans. Many aspects of a biological response cannot be reproduced with in vitro tests, including blood interaction, wound healing, hypersensitivity reactions, carcinogenesis, chronic inflammation. Animal experiments provide information about these types of effects without exposing humans to risk.  The aim of this study was to evaluate the biocompatibility of polymer objects on the basis of epoxy resins Accura® ClearVue ™ (chemical composition: 4.4' Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2.3-epoxypropane (40-65%), a mixture of triaryl sulfonium salt (50% propylene carbonate and 50% of a triarylsulfonium hexafluoroantimonate salt) (1- 10%) and 3-ethyl-3-hydroxymethyl-oxetane (10-20%). In the assessment of the cytotoxicity of materials Accura® ClearVue ™ agar diffusion and MTT tests were used. Both tests were conducted on cell cultures L929 (mouse fibroblasts) and MRC-5 (human fibroblasts). An assessment of the biocompatibility of the tested material was done on the basis of an oral mucosa irritation test on a hamster cheek pouch as defined by ISO 10993-10: 2010. The biocompatibility of the material was also tested with the implantation of a samples into the dorsal subcutaneous tissue of a Wistar rats. The subcutaneous implantation test, as one of the most reliable methods for assessing the biocompatibility of dental materials, is defined by ISO 10993-6: 2010. The study was conducted on 30 rats which were sacrificed in groups
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Curtis, Courtney Lee. "Wnt signaling in zebrafish fin regeneration : chemical biology using a GSK3β inhibitor." Thesis, 2014. http://hdl.handle.net/1805/4835.

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Indiana University-Purdue University Indianapolis (IUPUI)
Bone growth can be impaired due to disease, such as osteoporosis. Currently, intermittent parathyroid hormone (PTH) treatment is the only approved therapy in the United States for anabolic bone growth in osteoporosis patients. The anabolic effects of PTH treatment are due, at least in part, to modulation of the Wnt/β-catenin pathway. Activation of the Wnt/ β-catenin pathway using a small molecule inhibitor of GSK3β was previously shown to increase markers of bone formation in vitro. Our study utilized a zebrafish model system to study Wnt activated fin regeneration and bone growth. Wnt signaling is the first genetically identified step in fin regeneration, and bony rays are the main structure in zebrafish fins. Thus, zebrafish fin regeneration may be a useful model to study Wnt signaling mediated bone growth. Fin regeneration experiments were conducted using various concentrations of a GSK3β inhibitor compound, LSN 2105786, for different treatment periods and regenerative outgrowth was measured at 4 and 7 days post amputation. Experiments revealed continuous low concentration (4-5 nM) treatment to be most effective at increasing regeneration. Higher concentrations inhibited fin growth, perhaps by excessive stimulation of differentiation programs. In situ hybridization experiments were performed to examine effects of GSK3β inhibitor on Wnt responsive gene expression. Experiments showed temporal and spatial changes on individual gene markers following GSK3β inhibitor treatment. Additionally, confocal microscopy and immunofluorescence labeling data indicated that the Wnt signaling intracellular signal transducer, β-catenin, accumulates throughout GSK3β inhibitor treated tissues. Finally, experiments revealed increased cell proliferation in fin regenerates following LSN 2105786 treatment. Together, these data indicate that bone growth in zebrafish fin regeneration is improved by activating Wnt signaling. Zebrafish Wnt signaling experiments provide a good model to study bone growth and bone repair mechanisms, and may provide an efficient drug discovery platform.
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Walker, Chandler L. "Targeting acute phosphatase PTEN inhibition and investigation of a novel combination treatment with Schwann cell transplantation to promote spinal cord injury repair in rats." Thesis, 2014. http://hdl.handle.net/1805/4210.

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Indiana University-Purdue University Indianapolis (IUPUI)
Human traumatic spinal cord injuries (SCI) are primarily incomplete contusion or compression injuries at the cervical spinal level, causing immediate local tissue damage and a range of potential functional deficits. Secondary damage exacerbates initial mechanical trauma and contributes to function loss through delayed cell death mechanisms such as apoptosis and autophagy. As such, understanding the dynamics of cervical SCI and related intracellular signaling and death mechanisms is essential. Through behavior, Western blot, and histological analyses, alterations in phosphatase and tensin homolog (PTEN)/phosphatidylinositol-3-kinase (PI3K) signaling and the neuroprotective, functional, and mechanistic effects of administering the protein tyrosine phosphatase (PTP) inhibitor, potassium bisperoxo (picolinato) vanadium ([bpV[pic]) were analyzed following cervical spinal cord injury in rats. Furthermore, these studies investigated the combination of subacute Schwann cell transplantation with acute bpV(pic) treatment to identify any potential additive or synergistic benefits. Although spinal SC transplantation is well-studied, its use in combination with other therapies is necessary to complement its known protective and growth promoting characteristics. v The results showed 400 μg/kg/day bpV(pic) promoted significant tissue sparing, lesion reduction, and recovery of forelimb function post-SCI. To further clarify the mechanism of action of bpV(pic) on spinal neurons, we treated injured spinal neurons in vitro with 100 nM bpV(pic) and confirmed its neurprotection and action through inhibition of PTEN and promotion of PI3K/Akt/mammalian target of rapamycin (mTOR) signaling. Following bpV(pic) treatment and green fluorescent protein (GFP)-SC transplantation, similar results in neuroprotective benefits were observed. GFP-SCs alone exhibited less robust effects in this regard, but promoted significant ingrowth of axons, as well as vasculature, over 10 weeks post-transplantation. All treatments showed similar effects in forelimb function recovery, although the bpV and combination treatments were the only to show statistical significance over non-treated injury. In the following chapters, the research presented contributes further understanding of cellular responses following cervical hemi-contusion SCI, and the beneficial effects of bpV(pic) and SC transplantation therapies alone and in combination. In conclusion, this work provides a thorough overview of pathology and cell- and signal-specific mechanisms of survival and repair in a clinically relevant rodent SCI model.
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Books on the topic "Tissue engineering – laboratory manuals"

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Micou, Melissa Kurtis, and Dawn Kilkenny. A laboratory course in tissue engineering. Boca Raton: Taylor & Francis, 2012.

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Freshney, R. Ian. Culture of cells for tissue engineering. New York, NY: Wiley, 2006.

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Freshney, R. Ian. Culture of Cells for Tissue Engineering. New York: John Wiley & Sons, Ltd., 2006.

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Radisic, Milica, and Black Lauren D. Cardiac tissue engineering: Methods and protocols. New York: Humana Press, 2014.

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P, Hollander Anthony, and Hatton Paul V, eds. Biopolymer methods in tissue engineering. Totowa, N.J: Humana Press, 2004.

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Wright, Bernice. Corneal Regenerative Medicine: Methods and Protocols. Totowa, NJ: Humana Press, 2013.

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Birchler, James A. Plant chromosome engineering. New York, NY: Humana Press, 2011.

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Kioussi, Chrissa. Stem cells and tissue repair: Methods and protocols. New York: Humana Press, 2014.

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Shimizu, Tatsuya, Gilson Khang, Jan-Thorsten Schantz, and Kee-Woei Ng. A manual for differentiation of bone marrow-derived stem cells to specific cell types. New Jersey: World Scientific, 2014.

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Turksen, Kursad, and Gordana Vunjak-Novakovic. Biomimetics and stem cells: Methods and protocols. New York: Humana Press, 2014.

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Book chapters on the topic "Tissue engineering – laboratory manuals"

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Komath, Manoj, H. K. Varma, Annie John, Vinod Krishnan, Deepti Simon, Manikandhan Ramanathan, and G. S. Bhuvaneshwar. "Designing Bioactive Scaffolds for Dental Tissue Engineering." In Regenerative Medicine: Laboratory to Clinic, 423–47. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3701-6_25.

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Arora, Aditya, Arijit Bhattacharjee, Aman Mahajan, and Dhirendra S. Katti. "Cartilage Tissue Engineering: Scaffold, Cell, and Growth Factor-Based Strategies." In Regenerative Medicine: Laboratory to Clinic, 233–57. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3701-6_14.

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Gowanlock, Jordan. "Engineering Moving Images: “Tech Dev” Meets “Look Dev”." In Palgrave Animation, 85–117. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-74227-0_4.

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AbstractThis chapter investigates the way engineering principles have transformed cinematic visual effects and animation production since the 1980s, noting the growing influence of “agile” software development. Film industry training manuals, trade press articles, and interviews with workers show that the flexible, reprogrammable nature of production “workflows” requires constant work to maintain the technical connective tissue of “pipelines.” This emphasis on making and re-making custom tools for every production serves post-Fordist demands of flexibility. Automated simulation-based forms of animation offer a paradigmatic case for these trends, where a spectrum of technical work spans basic research done by lead scientists, through to the scripts written and plug-ins installed by simulation artists.
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Hagen, Charlotte K. "X-Ray Phase Contrast Tomography in Tissue Engineering: Focus on Laboratory Implementations." In Advanced High-Resolution Tomography in Regenerative Medicine, 217–32. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-030-00368-5_15.

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Terkowsky, Claudius, Marcel Schade, Konrad E. R. Boettcher, and Tobias R. Ortelt. "Once the Child Has Fallen into the Well, It is Usually Too Late Using Content Analysis to Evaluate Instructional Laboratory Manuals and Practices." In Open Science in Engineering, 11–23. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-42467-0_2.

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O’Donnell, B., A. Swarup, A. Sidiq, D. Robert, and S. Setunge. "Guidelines for Enzymatic Soil Stabilization." In Lecture Notes in Civil Engineering, 373–98. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-3330-3_40.

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AbstractThere are numerous manuals to guide practitioners in utilizing traditional additives in the construction of road, rail and dam construction but they fall short of specific guidance for non-standard additive-based ecofriendly and cost-effective soil stabilization. Increased attention has recently been on the use of non-standard additives for stabilizing weak soils due to environmental and cost concerns associated with traditional additives. We summarize the specific guidelines of using environmental-friendly enzymes to treat weak soils. We elaborate on the requirements and specifications for the Eko-Soil multi-enzyme product that is manufactured from water and proteins extracted from fermented exudes of plants. Specific tests (laboratory and field) and conditions required for soil stabilization using Eko-Soil enzyme are elaborated using the experience of past construction projects. The guide also elaborates enhancing the efficiency of enzymatic soil stabilization by correctly incorporating the required mixing proportions and pre-requisite condition tests. Professionals and practitioners will benefit from using novel eco-friendly sustainable stabilization techniques in the treatment of weak soils covering many applications including roads, foundations, water containment areas, landfills, working platforms and slope erosion control.
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"Module IV. Tissue Engineering." In A Laboratory Course in Biomaterials, 129–64. CRC Press, 2009. http://dx.doi.org/10.1201/b15832-7.

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H., Tarek. "Low Intensity Pulsed Ultrasound: A Laboratory and Clinical Promoter in Tissue Engineering Abstract." In Tissue Engineering. InTech, 2010. http://dx.doi.org/10.5772/8596.

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"- Decellularized Matrices for Tissue Engineering." In A Laboratory Course in Tissue Engineering, 100–111. CRC Press, 2016. http://dx.doi.org/10.1201/b12792-12.

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Nather, Aziz, Lee Choon Wei, and Tang Zhiqun. "Setting Up a Tissue Engineering Laboratory." In Bone Grafts And Bone Substitutes, 313–20. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812775337_0019.

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Conference papers on the topic "Tissue engineering – laboratory manuals"

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Raxworthy, Michael J., Lorenzo Pio Serino, and Peter D. Iddon. "Electrospun bioresorbable tissue repair scaffolds: From laboratory to clinic." In 2018 3rd Biennial South African Biomedical Engineering Conference (SAIBMEC). IEEE, 2018. http://dx.doi.org/10.1109/saibmec.2018.8363177.

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Bordegoni, Monica, Marina Carulli, and Elena Spadoni. "Multisensory VR for Delivering Training Content to Machinery Operators." In ASME 2021 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2021. http://dx.doi.org/10.1115/detc2021-69974.

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Abstract The issue of training operators in the use of machinery is topical in the industrial field and in many other contexts, such as university laboratories. Training is about learning how to use machinery properly and safely. Beyond the possibility of studying manuals to learn how to use a machine, operators typically learn through on-the-job training. Indeed, learning by doing is in general more effective, tasks done practically are remembered more easily, and the training is more motivating and less tiresome. On the other hand, this training method has several negative factors. In particular, safety may be a major issue in some training situations. An approach that may contribute overcoming negative factors is using Virtual Reality and digital simulations techniques for operators training. The research work presented in this paper concerns the development of a multisensory Virtual Reality environment for training operators to properly use machinery and Personal Protective Equipment (PPE). The context selected for the study is a university laboratory hosting manufacturing machinery. It has been developed an application that allows user to navigate the laboratory, to approach a machine and learn about how to operate it and also what PPE to use while operating. Specifically, the paper describes the design and implementation of the application.
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Hagen, Charlotte K., Panagiotis Maghsoudlou, Giorgia Totonelli, Paul C. Diemoz, Marco Endrizzi, Anna Zamir, Paola Coan, Alberto Bravin, Paolo De Coppi, and Alessandro Olivo. "Strategies for fast and low-dose laboratory-based phase contrast tomography for microstructural scaffold analysis in tissue engineering." In SPIE Optical Engineering + Applications, edited by Stuart R. Stock, Bert Müller, and Ge Wang. SPIE, 2016. http://dx.doi.org/10.1117/12.2237594.

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Briko, Andrey N., Alexander V. Kobelev, Alexey N. Tikhomirov, Ahmad M. Hammoud, Konstantin V. Kotenko, and Ilya I. Eremin. "Development of a Laboratory Stand for Automated Mechanical Production of Stromal-Vascular Fraction From Adipose Tissue." In 2024 6th International Youth Conference on Radio Electronics, Electrical and Power Engineering (REEPE). IEEE, 2024. http://dx.doi.org/10.1109/reepe60449.2024.10479817.

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Nagatomi, Jiro, Michael B. Chancellor, and Michael S. Sacks. "Active Biaxial Mechanical Properties of Bladder Wall Tissue." In ASME 2003 International Mechanical Engineering Congress and Exposition. ASMEDC, 2003. http://dx.doi.org/10.1115/imece2003-43146.

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The urinary bladder is a smooth muscle organ whose main functions are to store and to void urine. Since the most important aspect of the storage function of the bladder is to maintain low intravesical pressure in order to protect the upper urinary tract from backflow of urine, the compliance of the bladder wall is one of the key functional paramters to assess the health of this organ. Previously, our laboratory reported, for the first time, the biaxial mechanical properties of bladder wall tissue in the inactive state (in the absence of calcium in the testing bath solution and thus smooth muscle contraction was abolished) (Gloeckner et al. 2002). The bladder in vivo, however, normaly exhibits passive smooth muscle tone during filling and active contraction during voiding. Therefore, in order to completely characterize the bladder tissue mechanical behaviors, it is necessary to examine the load-deformation relationship of the bladder under the passive and active states. In the present study, a novel experimental model was designed to allow collection of biaxial stress-strain data from urinary bladder wall tissue under passive, active and inactive states.
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Akkus, Ozan, and Allison Sieving. "Laboratory Modules for Reinforcement of Concepts Taught in Undergraduate Tissue Mechanics Course." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192691.

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Tissue mechanics is one of the key courses of the Biomechanics subtrack of the undergraduate curriculum. The aims of the course include: 1) To understand the concepts of stress, strain, viscoelasticity and how these concepts apply to musculoskeletal tissues. 2) The ability to infer the state of stress and strain at a given point in a biological structure under torsional, axial, bending and other types of loads. 3) To understand the anatomy of musculoskeletal tissues. Accomplishment of these aims requires a holistic understanding of statics, strength of materials and microanatomy of connective tissues. Conveying this wide range of topics in one class is a major challenge and most textbooks on this subject lack depth either in engineering or in physiology. The purpose of this abstract is to describe the benefits of the integration of theory with experimental practice for bridging the difficult topics of statics, strength of materials and tissue anatomy within the framework of undergraduate biomedical engineering curriculum.
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Nirmalanandhan, Victor S., Kirsten R. C. Kinneberg, Natalia Juncosa-Melvin, Heather M. Powell, Marepalli Rao, Steven T. Boyce, and David L. Butler. "Evaluation of a Novel Scaffold Material for Tendon Tissue Engineering." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176294.

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Tissue engineering offers an attractive alternative to direct repair or reconstruction of soft tissue injuries. Tissue engineered constructs containing mesenchymal stem cells (MSCs) seeded in commercially available type I collagen sponges (P1076, Kensey Nash Corporation, Exton, PA) are currently being used within our laboratory to repair tendon injuries in rabbit models [1]. When introduced into the wound site, mechanically stimulated stem cell-collagen sponge constructs exhibit 50% greater maximum force and stiffness at 12 weeks compared to values for static controls [1]. However, these constructs often lack the maximum force sufficient to resist the peak in vivo forces acting on the repair site [2, 3]. Insufficient repair biomechanics can be attributed to the poor initial mechanical resistance provided by the collagen sponges to replace the function of the lost tendon before its degradation and replacement with new extracellular matrix. This current study seeks to identify a biologically-derived scaffold with improved mechanical integrity that could be used in stem cell-based tissue engineered constructs for tendon repair.
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Shah, Chirag S., Matthew J. Mason, King H. Yang, Warren N. Hardy, Chris A. Van Ee, Richard Morgan, and Kennerly Digges. "High-Speed Biaxial Tissue Properties of the Human Cadaver Aorta." In ASME 2005 International Mechanical Engineering Congress and Exposition. ASMEDC, 2005. http://dx.doi.org/10.1115/imece2005-82085.

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Traumatic rupture of the aorta (TRA) is one of the leading causes of mortality in automobile crashes. Finite element (FE) modeling, used in conjunction with laboratory experiments, has emerged as increasingly important tool to understand the mechanisms of TRA. Appropriate material modeling of the aorta is a key aspect of such efforts. The current study focuses on obtaining biaxial mechanical properties of aorta tissue at strain rates typically experienced during automotive crashes. Five descending thoracic aorta samples from human cadavers were harvested in a cruciate shape. The samples were subjected to equibiaxial stretch at a strain rate of 44 s−1 using a new biaxial tissue-testing device. Inertially compensated loads were measured. High-speed videography was used to track ink dots marked on the center of each sample to obtain strain. The aorta tissue exhibited anisotropic and nonlinear behavior. The tissue was stiffer in the circumferential direction with a modulus of 10.64 MPa compared to 7.94 MPa in longitudinal direction. The peak stresses along the circumferential and longitudinal directions were found to be 1.89 MPa and 1.76 MPa, respectively. The tissue behavior can be used to develop a better constitutive representation of the aorta, which can be incorporated into FE models of the aorta.
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Holmes, Jeffrey W. "A Matlab-Based Cardiac Mechanics Course: Exportable Tools for Graduate-Level Soft Tissue Biomechanics." In ASME 2001 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2001. http://dx.doi.org/10.1115/imece2001/bed-23021.

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Abstract Graduate courses in finite elasticity and soft tissue biomechanics must deliver substantial theoretical content to prepare students for the complexities of soft tissue mechanics. In the laboratory students often discover that practical tasks such as calculating three-dimensional finite strain from marker data or reconstructing three-dimensional marker positions from two-dimensional views are not obvious despite mastery of the underlying theory. This course sought to replace as much didactic time as possible with hands-on MATLAB programming and simulations to illustrate basic theory and help students make the bridge to practical applications.
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Simmons, D. J., M. Krukowski, L. X. Bi, and E. Mainous. "Positively and Negatively-Charged Ion Exchange Resins: Disparate Effects on Hard Tissue Repair." In ASME 1997 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1997. http://dx.doi.org/10.1115/imece1997-0310.

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Abstract Bioelectrical investigations have long shown that surfaces of bone formation and resorption are negatively and positively charged respectively. We also know that in a number of experimental situations [1], implants of negatively-charged ion exchange resin (NCR= Sephadex, CM)are osteotropic, and that implants of positively-charged resin (PCR= Sephadex DEAE) strongly inhibit bone formation [2]. While the cellular mechanism of action for NCR is thought to involve the local production of transforming growth factor beta [3], the mechanics of PCR action is an unknown. Our laboratory has shown that PCR stunts the in vitro growth of medullary osteoprogenitor cells, normal and transformed osteoblasts, and a number of tumor cell lines [4], PCR was also able to strongly inhibit hamster pancreatic cell engraftment and the growth of established pancreatic cell tumors.
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