Dissertations / Theses: 'Tissue culture'

1

Sheibani, Ahmad. "Tissue culture studies of Pistacia." Thesis, University of Salford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.238801.

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2

Arunyanart, Sumay. "Chrysanthemum improvement through tissue culture." Thesis, Arunyanart, Sumay (1988) Chrysanthemum improvement through tissue culture. PhD thesis, Murdoch University, 1988. https://researchrepository.murdoch.edu.au/id/eprint/51910/.

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The use of two in vitro techniques for Chrysanthemum improvement were studied. Firstly, the direction and extent of somaclonal variation was observed using different explants from cultivars with a range of flower shape and colour. Secondly, an attempt was made to produce chimaeras from mixed calluses. Tissue culture methods were developed for callus induction and shoot regeneration from 6 cultivars of Chrysanthemum morifolium and for C. carinaturn (Syn. C. tricolour), C. parthenium (Pyrethrum) and Aster novi-belqii (Michaelmas daisy) which were included in the chimaera work. Flower colour changes were seen among regenerated plants from red, pink and bronze but not from yellow and white cultivars. The direction of colour change was similar to that recorded from spontaneous mutation. Colour change variants were most frequent in plants regenerated from stem (internodal) or petal explants and least from bud explants. All cultivars showed variants in flower shape amongst regenerated plants. The yellow-flowered cultivars showed the greatest range of types of flower shape, variation in shape was less in the bronze, pink, red and white cultivars. The most distinctive variant types of flower shape were the single, quill and pompon types. The explant types giving the greatest variation in flower shapes were buds or petals. Overall, the proportion of normal true-to-type flowers obtained was highest using bud cultures. This could be because in bud cultures some buds retain their structure despite the development of callus, or because new shoots are produced much faster from bud callus than from petal or stem callus. The most successful method for inducing callus fusion was to place fresh explants in contact with each other side-by-side on the medium. Isozyme analysis was used to screen for chimaeric plants, combinations of parent' types being chosen for their distinctively different isozyme patterns. No regenerated plants showed the isozyme pattern of more than one 'parent', but some somaclonal variants in banding patterns of isozymes were obtained.

3

Brons, I. G. M. "Tissue culture of rat insulinoma cells." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303711.

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4

Rathbone, Sandra. "Dynamic culture of tissue engineered ligaments." Thesis, Keele University, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.534314.

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5

Cousineau, Johanne. "Isoenzyme studies and tissue culture of raspberry." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=70254.

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Starch gel electrophoresis and isoenzyme staining were studied in raspberry (Rubus idaeus L., R. X neglectus Peck, and R. occidentalis L.). Seven isoenzymes could be separated using one of two gel-electrophoresis buffers: tris-citric acid at pH 7.1 for aconitase (ACO), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), and triose phosphate isomerase (TPI) and histidine-citric acid at pH 5.7 for malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), and shikimate dehydrogenase (SKDH). There were no variations detected between samples obtained from micropropagated shoots, greenhouse-, or field-grown plants. Tissue type and age had no effect on isoenzyme banding patterns except for PGM where this affected the relative densities of the bands. Fifty-five out of 78 raspberry cultivars could be uniquely characterized using the above isoenzymes. Analysis of cultivars obtained from multiple sources detected occasional mislabelled plants. The mode of inheritance of raspberry isoenzymes was studied and analysis of co-segregating loci revealed two possible linkage groups: Mdh-2/Tpi-2/Pgm-1 and Idh-1/widh.
A high rate (70%) of adventitious shoot regeneration was observed from leaf-petiole explants of micropropagated shoot cultures of 'Comet' red raspberry cultured on modified Murashige-Skoog medium containing 1 mg/l thidiazuron (TDZ) and 0.5 mg/l 1H-indole-3-butanoic acid (IBA). Variation in the agar concentration or incubation temperature, orientation or scoring of the leaf-petiole explants and use of separate leaf or petiole explants had no effect on shoot regeneration while incubation in the dark for 1, 2, or 3 weeks prior to growth in the light depressed the number of adventitious shoots formed. Only 8 of 22 raspberry cultivars were capable of regenerating from leaf explants of greenhouse-grown plants.

6

Cubbin, Ian James. "Tissue culture studies in selected medicinal plants." Thesis, University of Sunderland, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320538.

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7

Aziz, Zaleha Biniti A. "Tissue culture of Centella asiatica : asiaticoside biosynthesis." Thesis, University of Nottingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368364.

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8

Tiwari, Vimlesh Kumar. "Tissue culture and transformation studies of barley." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334512.

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9

Brown, Andrew M. G. "Biochemistry, tissue culture and pharmacology of Tanacetum." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363563.

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10

El-Shaweesh, Kamal Husein. "Tissue culture and irradiation studies in Solanum." Thesis, University of Salford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334320.

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11

Longden, David. "Tissue culture induced changes in tobacco somaclones." Thesis, University of Liverpool, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306567.

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12

Al-Ani, Nabeel K. "Some epigenetic effects in plant tissue culture." Thesis, Aberystwyth University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.659362.

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13

Massa, Denise Marie. "Is Organic Tissue Culture of Petunia Possible?" Available to subscribers only, 2009. http://proquest.umi.com/pqdweb?did=1796330171&sid=6&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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14

Khan, Saifullah. "Variation arising through tissue culture in soft fruits." Thesis, University of Edinburgh, 1997. http://hdl.handle.net/1842/28357.

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The study mainly concerns the potential of the tissue culture for mass propagation and to evaluate the trueness to type of regenerated plants from tissue culture using appropriate molecular markers. Three soft fruits species Ribes, Fragaria and Rubus were used in this study. The study has three main sections dealing with an evaluation of appropriate molecular marker systems to study the plants regenerated via micropropagation, callus culture and regeneration and tissue culture and regeneration. The first section evaluates the potential of SDS-PAGE protein electrophoresis and RAPD-PCR as markers to distinguish among clonally propagated cultivars of R.nigrum. SDS-PAGE was able to distinguish only four out of ten cultivars tested. RAPD-PCR was able to distinguish all the cultivars studied using only two primers. The data generated by RAPD-PCR and from pedigree information was used to examine the relatedness among the cultivars studied. RAPD-PCR was further used to examine the purity of the cultivar Baldwin collected at various locations in the UK. Polymorphism was detected and differences were found between the sub samples of a single cultivar. The second section deals with the multiplication of Rubus, Ribes and Fragaria by micropropagation. The effect of culture cycle on the plants regenerated was evaluated using RAPD-PCR. Ribes did not show any variation until the 14^th generation cycle but in the 15^th and 16^th cycles variation was detected from 6.2% and 13.4% respectively. Considerable variation was detected in Rubus starting with the 4^th subculture and was at maximum in the 7^th subculture. In Fragaria, all plants at subculture 3 were evaluated and variation was detected between them. The relevance of such variation on the release of material of all the release species is discussed in relation to certification scheme requirements. The third experimental section evaluates the potential of callus as explant source for the multiplication and regeneration of plants in Ribes and Fragaria species.

15

Afshar-Sterle, Shoukat. "Cell, tissue culture and transformation of Triticum tauschii /." Title page, abstract and contents only, 2000. http://web4.library.adelaide.edu.au/theses/09APSP/09apspa258.pdf.

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16

Akhtar, Lal Hussain. "Tissue culture and stress tolerance in Gossypium species." Thesis, Bangor University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296184.

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17

Sipen, Philip. "Tissue Culture and Cryopreservation of Banana {Musa spp.)." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.523611.

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18

倪建春 and Kin-chun Ngai. "Demonstration of bilirubin cytotoxicity by tissue culture system." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1996. http://hub.hku.hk/bib/B31214526.

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19

Anjum, M. A. "Tissue culture and frost tolerance studies in Solanum." Thesis, University of Salford, 1994. http://usir.salford.ac.uk/14833/.

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In vitro shoot cultures of two commercial cultivars of Solanum tuberosum, Desiree and Mans Piper, and of two wild species S. commersonil and S. acaule, were established from single nodal explants and seedling tissues respectively. Callus cultures were initiated from potato stem, leaf and tuber explants. Cell suspension cultures were obtained from tuber and stem-derived calluses of S. tuberosum. Protoplasts were isolated from leaves of greenhouse-grown plants and from suspension-cultured cells of S. tuberosum, and in vitro shoot cultures of S. tube rosum and S. commersonii. Plantlets were regenerated from tuber discs, internodal explants, growing calluses, suspension-cultured cells and protoplast-derived calluses. Microtubers were induced from nodal explants of these Solanum species. Attempts were made to select frost-tolerant cell lines through resistance to hydroxyproline, by direct transfer of axillary buds, callus cultures and suspensioncultured cells to media containing different concentrations of hydroxyproline. Selection was also made after exposure of suspension-cultured cells to a freezing temperature (-6° C), and to gamma-irradiation (20 Gy). Several hyp-resistant cell lines were established from callus cultures and suspension-cultured cells but not from axillary buds. Most of these selected cell lines were found to show increased tolerance to frost. Plants were regenerated from one of the hyp-tolerant, frosttolerant cell lines. The cellular damage to S. tuberosum cv. Desiree callus cells due to freezing temperatures was examined, and the cellular structure of the callus of three Solanum species and one frost-tolerant cell line was compared by electron microscopy.

20

Tan, Chia Lock. "Tissue culture and genetic transformation of Theobroma cacao." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310835.

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21

Deljou, Ali. "Tissue culture and genetic transformation in potato breeding." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339661.

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22

Kermanee, Prasart. "Tissue culture and genetic manipulation of Thai rice." Thesis, University of Nottingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368338.

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23

James, V. J. "Regulation of xenobiotic catabolism in plant tissue culture." Thesis, Cardiff University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380205.

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24

Ge, Cheng. "Novel technologies for cell culture and tissue engineering." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:ab1014cf-80a4-4675-b607-96dc52c39b17.

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Cell culture has been a fundamental tool for the study of cell biology, tissue engineering, stem cell technology and biotechnology in general. It becomes more and more important to have a well-defined physiochemical microenvironment during cell culture. Conventional cell cultures employ expensive, manually controlled incubation equipment, making it difficult to maximize a cultures yield. Furthermore, previous studies use qualitative methods to assess cell culture proliferation that are inherently inaccurate and labour intensive, thereby increasing the cost of production. In addition, three dimensional cell culture, in scaffold, has been shown to provide more physiological relevant information as it mimic more accurate conditions that are similar to the physiological conditions of the human body compared with two dimension, which has special interest to regenerative medicine. Therefore, a portable and automated total-analysis-system (Î¼TAS) was proposed with microenvironment control and quantitative analysis techniques to monitor cell proliferation and metabolic activity. The automated portable heating system was validated to be capable to maintain a stable physiochemical microenvironment, with little margin of error, for cellular substrate outside of conventional incubation. A standalone platform system was designed and fabricated with accurate temperature control by employing an optically transparent ITO-film with a large heating area. The transparency of the film is critical for continuous in-situ microscopic observation over long-term cell culture process. Previous studies have attempted to use ITO-film as a heating element, but were unable to distribute the heat evenly onto the microbioreactor platform. This nagging problem in the literature was improved through a novel film design. As a result, the ITO-film based heating system was evaluated and constructed successfully to serve as a heating element for long-term static cell culture with facilitated proliferation rate in gas-permeable PDMS microbioreactor outside of conventional incubation. In addition to maintaining a stable microenvironment, a non-invasive in-situ technology for monitoring cell viability and proliferation rate was constructed and developed based on bioimpedance spectroscopy (BIS). It was primarily focused on making decisions for structure and specification of proposed system-on a chip BIS measurement. The miniaturization of BIS system on microbioreactor platform was achieved by utilizing and integrating switching matrix array, impedance analyzer chip with reliable analogue-front-end circuitry. The realized system was verified with the DLD-1 cells and its monitored data were validated with conventional bioassays. Three dimensional cell cultures with scaffold is a key to the success of tissue engineering. Engineered cornea collagen scaffold may be feasible using re-seeding proper human cells onto a decellularized corneal scaffold. The quality of the scaffold and the interaction of the cells are critical to the key function (i.e transparency, haze and total transmittance) of final products. An integrated corneal collagen scaffold quality assessment system, via optical property inspection unit, was innovatively designed and realized with non-invasive and non-destructive characteristics. The H1299 cells were seeded onto inspected corneal scaffold and BIS system, which were realized in the previous chapter, were used to validate its applicability for 3D cell culture. The cell adhesion as an outcome at different scaffolds with different optical properties has revealed the importance of the microstructure of scaffold on the cell functions. The results showed the developed technologies can be used for the quality control of corneal scaffold and the fabricated Î¼TAS not only enabled environmental control but, with BIS-based in-situ assay, it also facilitate the function (i.e adhesion) and viability monitoring with quantitative and qualitative analysis in 3D-alike cell culture. Additionally, by considering its low decontamination and cost-effective nature with compatibility for high-throughput screening applications, the fabricated and integrated systems has significant applications in tissue engineering.

25

Bonfils, Anne-Christine D. (Anne-Christine Dominique) Carleton University Dissertation Biology. "Tissue culture and intergeneric somatic hybridization in Brassicaceae." Ottawa, 1992.

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26

Ngai, Kin-chun. "Demonstration of bilirubin cytotoxicity by tissue culture system /." Hong Kong : University of Hong Kong, 1996. http://sunzi.lib.hku.hk/hkuto/record.jsp?B18155030.

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27

Baker, William P., Tyrone Harvard Hanks, and Louis Eduardo Marin. "Tissue Culture and Cloning of Carnegiea gigantea, Cactaceae." University of Arizona (Tucson, AZ), 2015. http://hdl.handle.net/10150/554346.

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Cloning has become an established method of supplying valuable timber trees and other plants for commercial purposes. Cloning of these plants allows multiple copies to be produced from superior phenotypes. In this study, in vitro clones were produced from phenotypically selected, commercially available saguaro (Carnegiea gigantea). The clones were produced from tissue plugs obtained from surface sterilized saguaro. The plugs were transferred using standard aseptic technique to culture dishes containing solid Callus Initiation Medium (Gamborg's B-5 medium supplemented with 10 mg/l auxin and 8 g/l agar). The cultures were incubated under continuous cool fluorescent lights at 24 C until callus formation was observed. Healthy callus were transferred to solid Development Medium (Gamborg's B-5 medium supplemented with 10 mg/l auxin, 10.0 mg Kinetin, and 8 g/l agar) and further incubated. Resulting clones were prepared for in vivo conditions by transfer to sterile potting soil and successfully outplanted to the green house. Such clones may supply scarce C. gigantea for future research. The use of single genotypes for ecological applications should be avoided since they lack natural population variability.

28

Wilson, Christopher G. "Modeling the dynamic composition of engineered cartilage." Link to electronic thesis, 2002. http://www.wpi.edu/Pubs/ETD/Available/etd-0326102-204208/.

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29

Charles, Lara Nicole. "Culture of cells from mammalian tissue cryopreserved without cryoprotection." [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-2819.

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30

Taeb, Abdulkarim Giumaa. "Influence of culture environment on tulip micropropagation." Thesis, University of Nottingham, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328788.

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31

Garber-Goldsman, Cheryl. "Studies of a central noradrenergic system in tissue culture." Thesis, University of Ottawa (Canada), 1985. http://hdl.handle.net/10393/4981.

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32

Batur, Sule. "Bacterial adhesion to endothelial cells in tissue culture systems." Thesis, Georgia Institute of Technology, 1991. http://hdl.handle.net/1853/25406.

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33

O'Dea, Reuben. "Multiphase modelling of tissue growth in dynamic culture conditions." Thesis, University of Nottingham, 2008. http://eprints.nottingham.ac.uk/10446/.

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In this thesis, a series of mathematical models suitable for describing biological tissue growth are developed. The motivation for this work is a bioreactor system which provides perfusion and compressive mechanical stimulation to a cell-seeded scaffold; however, the formulation is sufficiently general to be applied to a vast range of tissue engineering applications. Our models are used to investigate the influence of (i) cell-cell and cell-scaffold interactions, and (ii) the mechanical environment, on tissue growth. In the first part of the thesis, we extend a model due to Franks (2002) (in which the cell and culture medium phases are represented by viscous fluids) by including perfusion and coupling the cells' response to their environment. Specifically, we consider the effect of the cell density and pressure on tissue growth. We analyse the model using analytic and numerical techniques; numerical simulations suggest that comparison of construct morphology in the presence and absence of perfusion provides a means to identify the dominant regulatory growth stimulus. The solid characteristics of the construct and interactions between the cells and scaffold are necessarily neglected in the two phase model. Guided by this, we develop more complex three phase models. Using numerical simulations, the influence of cell-cell and cell-scaffold interactions is investigated and less porous scaffolds are shown to improve control over cell behaviour. We use the model to compare the cells' response to different regulatory stimuli, including flow-induced shear stress. Our results suggest that uniform initial cell seeding and stimulating cell movement are crucial in maintaining the mechanical integrity of tissue constructs. We also study the effect of scaffold compression on the mechanical environment of the cells contained within, developing both a classical Biot formulation and a multiphase model. We demonstrate that the bioreactor geometry introduces significant spatial variation in the mechanical stimuli relevant to tissue growth and that such considerations will play a key role in comprehensive models of mechanotransduction-affected growth.

34

Cancino, Escalante Giovanni Orlando. "Tissue culture and Agrobacterium-mediated transformation studies in Passiflora." Thesis, University of Nottingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342022.

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35

Craig, Wendy. "Tissue culture of Brassiceae : a basis for genetic improvement." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307757.

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36

Wardrop, Julie. "Biotechnological applications of perfluorochemical liquids in plant tissue culture." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389475.

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37

Khalid, Norzulaani. "Somaclonal variation through tissue culture studies in Chrysanthemum morifolium." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329847.

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38

Castellanos, Marcos. "Tissue culture and transformation of poinsettia (Euphorbia pulcherrima Willd.)." Thesis, University of Nottingham, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439998.

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39

Ghazvini, Reza Fotouhi. "Tissue culture and frost tolerance studies in Citrus spp." Thesis, University of Salford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308133.

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40

Wright, Rachel Claire. "Promoter probing of Listeria monocytogenes during tissue culture infection." Thesis, University of Warwick, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409907.

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41

Okyere-Boateng, George. "Tissue culture response in oil palm : (Elaeis guineensis Jacquin)." Thesis, University of Reading, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.395176.

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42

Chen, Ting-yu, and 陳婷玉. "Tissue Culture of Six Paphiopedilums." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/63898036664908086425.

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碩士
國立臺灣大學
園藝學研究所
88
Protocorm explants of Paphiopedilum delenatii were cultured on 1/2-MS medium containing 0-10 mg/L NAA and 0-10 mg/L kinetin. The plain basal medium (devoid any hormones additive) were found as the best medium for shood bud formation. NAA and kinetin inhibited shoot bud development of the protocorms. Shoot buds derived maining from the epidermal cells of the protocorm explants without intermediate calli form. The method can be used to shorten the period of bud proliferation and breeding. The leaf explants of Paphiopedilum callosum album ''Oakhill'' × lawrenceanum album ''Tradition'' formed yellow calli on the medium containing 5 mg/L 2,4-D and 1 mg/L TDZ in 20 weeks those leaf-derived calli could be subcultured on the same medium but failed to produce plantlets. Difference on percentage of callus inducing and morphogenesis were among 5 species tested. Calli from leaf explants had lower regeneration and died easily in subculture period. Further investigation should be focus on the problems. The percentage of shoot bud inducing had distinction in Paphiopedilum philippinense (PH59、PH60, these two came from different parents) may resulted mainly on genetic variation. The bud formation frequency of cutting-leaf explants were higher than intact leaves. These shoot-buds formed from leaf base developed into normal plantlets on plain basal medium. Stem node explants of Paphiopedilum philippinense were cultured in light on 1/2-MS medium. After 12 weeks, shoot buds formed directly from nodes. The buds transfered into free medium after 20 weeks. Some buds became muliple shoots and developed into plantlets with roots. The percentage of bud inducing could reach 80 ﹪. Calli derived from protocorms of Paphiopedilum callosum album ''Oakhill'' × lawrenceanum album ''Tradition'' subcultured on 1/2-MS medium containing 5 mg/L 2,4-D and 1 mg/L TDZ for three years were cultured in light on 1/2-MS medium adding 0.5 mg/L BPA formed shoot buds. This proved these calli retained totipotency in long-term subculture. Cytokinins ( kinetin、zeatin、BA、2iP and BPA ) retarded browning of calli. GA3、TIBA and ABA inhibited calli growth.

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CAI, ZONG-RUI, and 蔡宗叡. "Tissue culture of eucalyptus saligna." Thesis, 1991. http://ndltd.ncl.edu.tw/handle/00478117284774190243.

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Huang, Chien-Chen, and 黃千真. "Tissue culture of Aleurites montana." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/18407499483450231338.

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碩士
國立臺灣大學
森林環境暨資源學研究所
101
The Mature and non-mature seeds of Aleurites montana were used in this study, after arterialized with 1% sodium hypochlorite solution for 10 minutes. The seeds were cultured in MS culture medium, treated at 25℃ with a photoperiod of 16 hours and a light intensity of 28μEm-2s-1，which would result in 100% aseptic seedling. Cutting the stem of aseptic seedling into a length of 0.5~1.0 cm, cultured the samples in MS culture medium with 4mg/IBA for 4 weeks, which could induce the green callus, then taking 0.5cm3 sample from the green callus, cultured the sample in MS culture medium with 4mg/IBA, treated with a photoperiod of 16 hours, which could induce 97% multi-buds after 8 weeks. Cutting roots from the aseptic seedlings, the roots were cultured in MS culture medium with 4mg/IBA, treated with a photoperiod of 16 hours, after 4 weeks, each explants could induce 48.2 somatic embryos, if sub-cultured in the same culture medium and with the same treatment, which could produce secondary somatic embryo; putting somatic embryo in the culture medium with 0.1mg/IBA, which would promote the maturation of somatic embryo and conversion ratio. Taking 2.0mg/l green and tight callus, putting in 50ml liquid culture medium, in every 7 days, it was sub-cultured under the conditions of old liquid : new liquid = 1:5, which would increase to 3.3mg/l, and appear the formation of cell with dense cytoplasm.

45

Lo, Kuo~Jui, and 羅國瑞. "Tissue Culture of Taraxacum formosanum." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/69730092196932460564.

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碩士
中國醫藥學院
中國藥學研究所
89
Summary Taraxacum spp (Compositae) were first recorded in Sin-Sho-Pen-Tsao of the Tang Dynasty under inferior category.Taraxacum formosanum is one of the trantional Chinese medicinal herbs used in the treatment of matitis, tonsillits, cholestasis and cleaning the waste of the liver. Due to limited geographical distribution and indiscriminate collection, T. formosanum is now rarely found in its natural habitat. Over development of land for many use and introduction of alien species of T. officinale also resulted in reduction population of T. formosanum. The purpose of this study was to establish the tissue culture techniques for mass propagation of Taraxacum formosanum. The results of the present investigation are summarized as: 1.Nearly 100% germination rate was obtained, when seeds of T. formosanum were cultured on a medium containing half-strength MS basic salts supplemented with 0.5mg/l BA, 3% sucrose and 1.0% Difco agar. 2.A medium containing half-strength MS salts, 1.0 mg/l NAA, 0.5 mg/l BA, 3% sucrose and 1% Difco agar can induce callus from T. formosanum seed. 3.A medium containing half-strength MS salts, 0.5 mg/l NAA, 1.0 mg/l kinetin, 3% sucrose and 1.0% Difco agar (pH=5.7) was the most suitable medium for inducing callus formation of T. formosanum. The cultures were maintained at 25±1℃ under dark. 4.Addition of 10%(v/v) coconut milk (CM) or potato homogenate is not good for callus growth of T. formosanum. 5.Adding (500,1000,2000 mg/l) peptone or casein hydrolysate(CH) is not good for callus growth of T. formosanum. 6.Adventitious buds can be induced from leaves when cultured on a medium containing half-strength MS basic salts, 0.5mg/l BA, 3% sucrose and 1% Difco agar (pH=5.7). The cultures were maintained at 25±1℃ under a photoperiod of 14-h light (2000 lux fluorecent light) and 10-h dark cycle. 7.For rooting of adventitious buds, which are induced on leaves, buds were cultured on a medium containing 1/4~1/2 MS basic salts, 0.5mg/l NAA, 3~5% sucrose and 1% Difco agar (pH=5.7). The cultures were maintained at 25±1℃ under a photoperiod of 14-h light (2000 lux fluorecent light) and 10-h dark cycle. 8.The plantlets of T. formosanum produced from tissus culture could be transplanted into a mixture of vermiculite and peat moss (1:1), and grown successfully under outdoor conditions.

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Hung, Ming-Hung, and 洪銘宏. "Tissue culture of Taxus mairei." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/15619472501620853743.

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高毓瑩. "Tissue Culture of Zelkova Serrate." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/85438558056548564320.

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劉祖惠. "The tissue culture of Dieffenbachia." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/83314905429411437685.

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王上仁. "Tissue culture of Cotyledon tomentosa." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/3trs28.

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SHEN, MIN-JUAN, and 沈敏娟. "Tissue culture of ginkgo biloba." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/99777319907932899101.

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