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Jia, Zhidong, Yuan Cheng, Xinan Jiang, Chengyan Zhang, Gaoshang Wang, Jiecheng Xu, Yang Li, Qing Peng, and Yi Gao. "3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity." BioMed Research International 2020 (March 4, 2020): 1–22. http://dx.doi.org/10.1155/2020/6354183.
In vitro 3D hepatocyte culture constitutes a core aspect of liver tissue engineering. However, conventional 3D cultures are unable to maintain hepatocyte polarity, functional phenotype, or viability. Here, we employed microfluidic chip technology combined with natural alginate hydrogels to construct 3D liver tissues mimicking hepatic plates. We comprehensively evaluated cultured hepatocyte viability, function, and polarity. Transcriptome sequencing was used to analyze changes in hepatocyte polarity pathways. The data indicate that, as culture duration increases, the viability, function, polarity, mRNA expression, and ultrastructure of the hepatic plate mimetic 3D hepatocytes are enhanced. Furthermore, hepatic plate mimetic 3D cultures can promote changes in the bile secretion pathway via effector mechanisms associated with nuclear receptors, bile uptake, and efflux transporters. This study provides a scientific basis and strong evidence for the physiological structures of bionic livers prepared using 3D cultures. The systems and cultured liver tissues described here may serve as a better in vitro 3D culture platform and basic unit for varied applications, including drug development, hepatocyte polarity research, bioartificial liver bioreactor design, and tissue and organ construction for liver tissue engineering or cholestatic liver injury.
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Shrestha, Sunil, Vinod Kumar Reddy Lekkala, Prabha Acharya, Darshita Siddhpura, and Moo-Yeal Lee. "Recent advances in microarray 3D bioprinting for high-throughput spheroid and tissue culture and analysis." Essays in Biochemistry 65, no. 3 (August 2021): 481–89. http://dx.doi.org/10.1042/ebc20200150.
Abstract Three-dimensional (3D) cell culture in vitro has proven to be more physiologically relevant than two-dimensional (2D) culture of cell monolayers, thus more predictive in assessing efficacy and toxicity of compounds. There have been several 3D cell culture techniques developed, which include spheroid and multicellular tissue cultures. Cell spheroids have been generated from single or multiple cell types cultured in ultralow attachment (ULA) well plates and hanging droplet plates. In general, cell spheroids are formed in a relatively short period of culture, in the absence of extracellular matrices (ECMs), via gravity-driven self-aggregation, thus having limited ability to self-organization in layered structure. On the other hand, multicellular tissue cultures including miniature tissues derived from pluripotent stem cells and adult stem cells (a.k.a. ‘organoids’) and 3D bioprinted tissue constructs require biomimetic hydrogels or ECMs and show highly ordered structure due to spontaneous self-organization of cells during differentiation and maturation processes. In this short review article, we summarize traditional methods of spheroid and multicellular tissue cultures as well as their technical challenges, and introduce how droplet-based, miniature 3D bioprinting (‘microarray 3D bioprinting’) can be used to improve assay throughput and reproducibility for high-throughput, predictive screening of compounds. Several platforms including a micropillar chip and a 384-pillar plate developed to facilitate miniature spheroid and tissue cultures via microarray 3D bioprinting are introduced. We excluded microphysiological systems (MPSs) in this article although they are important tissue models to simulate multiorgan interactions.
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Şükrüoğlu Erdoğan, Özge, Seda Kılıç Erciyas, Ayhan Bilir, Şeref Buğra Tunçer, Demet Akdeniz Ödemiş, Sıdıka Kurul, Hasan Karanlık, Neslihan Cabıoğlu, and Hülya Yazıcı. "Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma." BioMed Research International 2019 (January 17, 2019): 1–9. http://dx.doi.org/10.1155/2019/1407167.
Epigenetic changes have major role in the normal development and programming of gene expression. Aberrant methylation results in carcinogenesis. The primary objective of our study is to determine whether primary tumor tissue and cultured tumor cells in 2D and 3D tissue culture systems have the same methylation signature forPAX5,TMPRSS2, andSBDS. These findings will play an important role in developing in vitro model system to understand the effect of methylation inhibitors on primary tumor tissue. In a previous studyPAX5,TMPRSS2, andSBDSgenes that we are investigating were reported to be methylated more than 60% in breast cancer and malignant melanoma cell lines. However, these genes have never been studied in primary tumor tissues. Thus, primary tumor tissues of breast cancer and malignant melanoma were first grown in 2D and 3D cultures. Then these two types of tumor tissues and their 2D and 3D cultures were investigated for changes considering methylation levels inPAX5,TMPRSS2, andSBDSgenes using real-time polymerase chain reaction. No differences were observed in the primary tissues and culture systems for bothPAX5andTMPRSS2in malignant melanoma tissues. We found thatPAX5gene was an efficient marker to measure the effects of methylation inhibitors for in vitro systems for malignant melanoma tissue.
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Peel, Trisha N., Tim Spelman, Brenda L. Dylla, John G. Hughes, Kerryl E. Greenwood-Quaintance, Allen C. Cheng, Jayawant N. Mandrekar, and Robin Patel. "Optimal Periprosthetic Tissue Specimen Number for Diagnosis of Prosthetic Joint Infection." Journal of Clinical Microbiology 55, no. 1 (November 2, 2016): 234–43. http://dx.doi.org/10.1128/jcm.01914-16.
ABSTRACTWe recently demonstrated improved sensitivity of prosthetic joint infection (PJI) diagnosis using an automated blood culture bottle system for periprosthetic tissue culture [T. N. Peel et al., mBio 7(1):e01776-15, 2016,https://doi.org/10.1128/mBio.01776-15]. This study builds on the prior research by examining the optimal number of periprosthetic tissue specimens required for accurate PJI diagnosis. Current guidelines recommend five to six, which is impractical. We applied Bayesian latent class modeling techniques for estimating diagnostic test properties of conventional culture techniques (aerobic and anaerobic agars and thioglycolate broth) compared to inoculation into blood culture bottles. Conventional, frequentist receiver operating characteristic curve analysis was conducted as a sensitivity analysis. The study was conducted at Mayo Clinic, Rochester, MN, from August 2013 through April 2014 and included 499 consecutive patients undergoing revision arthroplasty from whom 1,437 periprosthetic tissue samples were collected and processed. For conventional periprosthetic tissue culture techniques, the greatest accuracy was observed when four specimens were obtained (91%; 95% credible interval, 77 to 100%), whereas when using inoculation of periprosthetic tissues into blood culture bottles, the greatest accuracy of diagnosis was observed when three specimens were cultured (92%; 95% credible intervals, 79 to 100%). Results of this study show that the greatest accuracy of PJI diagnosis is obtained when three periprosthetic tissue specimens are obtained and inoculated into blood culture bottles or four periprosthetic tissue specimens are obtained and cultured using standard plate and broth cultures. Increasing the number of specimens to five or more, per current recommendations, does not improve accuracy of PJI diagnosis.
The use of two in vitro techniques for Chrysanthemum improvement were studied. Firstly, the direction and extent of somaclonal variation was observed using different explants from cultivars with a range of flower shape and colour. Secondly, an attempt was made to produce chimaeras from mixed calluses.
Tissue culture methods were developed for callus induction and shoot regeneration from 6 cultivars of Chrysanthemum morifolium and for C. carinaturn (Syn. C. tricolour), C. parthenium (Pyrethrum) and Aster novi-belqii (Michaelmas daisy) which were included in the chimaera work.
Flower colour changes were seen among regenerated plants from red, pink and bronze but not from yellow and white cultivars. The direction of colour change was similar to that recorded from spontaneous mutation. Colour change variants were most frequent in plants regenerated from stem (internodal) or petal explants and least from bud explants.
All cultivars showed variants in flower shape amongst regenerated plants. The yellow-flowered cultivars showed the greatest range of types of flower shape, variation in shape was less in the bronze, pink, red and white cultivars. The most distinctive variant types of flower shape were the single, quill and pompon types. The explant types giving the greatest variation in flower shapes were buds or petals.
Overall, the proportion of normal true-to-type flowers obtained was highest using bud cultures. This could be because in bud cultures some buds retain their structure despite the development of callus, or because new shoots are produced much faster from bud callus than from petal or stem callus.
The most successful method for inducing callus fusion was to place fresh explants in contact with each other side-by-side on the medium. Isozyme analysis was used to screen for chimaeric plants, combinations of parent' types being chosen for their distinctively different isozyme patterns. No regenerated plants showed the isozyme pattern of more than one 'parent', but some somaclonal variants in banding patterns of isozymes were obtained.
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Brons, I. G. M. "Tissue culture of rat insulinoma cells." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303711.
Starch gel electrophoresis and isoenzyme staining were studied in raspberry (Rubus idaeus L., R. X neglectus Peck, and R. occidentalis L.). Seven isoenzymes could be separated using one of two gel-electrophoresis buffers: tris-citric acid at pH 7.1 for aconitase (ACO), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), and triose phosphate isomerase (TPI) and histidine-citric acid at pH 5.7 for malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), and shikimate dehydrogenase (SKDH). There were no variations detected between samples obtained from micropropagated shoots, greenhouse-, or field-grown plants. Tissue type and age had no effect on isoenzyme banding patterns except for PGM where this affected the relative densities of the bands. Fifty-five out of 78 raspberry cultivars could be uniquely characterized using the above isoenzymes. Analysis of cultivars obtained from multiple sources detected occasional mislabelled plants. The mode of inheritance of raspberry isoenzymes was studied and analysis of co-segregating loci revealed two possible linkage groups: Mdh-2/Tpi-2/Pgm-1 and Idh-1/widh. A high rate (70%) of adventitious shoot regeneration was observed from leaf-petiole explants of micropropagated shoot cultures of 'Comet' red raspberry cultured on modified Murashige-Skoog medium containing 1 mg/l thidiazuron (TDZ) and 0.5 mg/l 1H-indole-3-butanoic acid (IBA). Variation in the agar concentration or incubation temperature, orientation or scoring of the leaf-petiole explants and use of separate leaf or petiole explants had no effect on shoot regeneration while incubation in the dark for 1, 2, or 3 weeks prior to growth in the light depressed the number of adventitious shoots formed. Only 8 of 22 raspberry cultivars were capable of regenerating from leaf explants of greenhouse-grown plants.
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Cubbin, Ian James. "Tissue culture studies in selected medicinal plants." Thesis, University of Sunderland, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320538.
Aziz, Zaleha Biniti A. "Tissue culture of Centella asiatica : asiaticoside biosynthesis." Thesis, University of Nottingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368364.
Tiwari, Vimlesh Kumar. "Tissue culture and transformation studies of barley." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334512.
Brown, Andrew M. G. "Biochemistry, tissue culture and pharmacology of Tanacetum." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363563.
El-Shaweesh, Kamal Husein. "Tissue culture and irradiation studies in Solanum." Thesis, University of Salford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334320.
Priyadarshan, P. M. "Tissue Culture." In PLANT BREEDING: Classical to Modern, 475–91. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-7095-3_21.
Taylor, N. L., and K. H. Quesenberry. "Tissue Culture." In Red Clover Science, 170–87. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-015-8692-4_14.
Gooch, Jan W. "Tissue Culture." In Encyclopedic Dictionary of Polymers, 928. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14970.
Rupert, E. A., and G. B. Collins. "Tissue Culture." In Agronomy Monographs, 405–16. Madison, WI, USA: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, 2015. http://dx.doi.org/10.2134/agronmonogr25.c16.
Loyola-Vargas, Víctor M., C. De-la-Peña, R. M. Galaz-Ávalos, and F. R. Quiroz-Figueroa. "Plant Tissue Culture." In Springer Protocols Handbooks, 875–904. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_50.
Paranjothy, K. "Hevea Tissue Culture." In Cell and Tissue Culture in Forestry, 326–37. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0992-7_25.
Yongkun Yu and Qingpeng Sun. "Aloe tissue culture technology." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5965875.
Li, Nan-Yu, Hao-Ru Tang, Cong Ge, Fan Mo, Yi-Hu Xiao, and Ya Luo. "Tissue culture of Lavandula angustifolia L." In 2018 INTERNATIONAL CONFERENCE ON BIOTECHNOLOGY AND BIOENGINEERING (8TH ICBB). Author(s), 2019. http://dx.doi.org/10.1063/1.5092392.
Pivovarova, N. S., T. S. Shebitchenko, and A. G. Podboronova. "Obtaining tissue culture of Scutellaria baicalensis." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.198.
The work is devoted to the obtaining of callus culture of Scutellaria baicalensis from sterile microcuttings. Cultivation conditions were determined, growth activity was studied, and a qualitative analysis was carried out.
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Suganuma, Lisa, Hiromichi Fujie, Hiroki Sudama, Yoshihide Sato, Norimasa Nakamura, Kenji Suzuki, Yasuhiro Tanaka, and Nobuyuki Moronuki. "Nanostructure Processed on Culture Plate Improves Cell Adhesion." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53753.
Ligaments and tendons have superior functions, but their healing capacities are limited. We have been developing a novel tissue-engineering technique for the repair of ligaments and tendons which involve stem cell-based self-assembled tissues (scSAT) derived from synovium[1]. For biological reconstruction of soft tissues, it is required for the scSAT to have high tensile strength. Our previous study indicted that, when the scSAT was cultured under high cell density condition, the tensile strength of the scSAT become higher than that cultured under low density condition[2]. However, the scSAT had a significant tendency to detach naturally from the culture dish with increasing cell density. Therefore, we expect that the mechanical property of the scSAT improves by enhancing the cell adhesion to culture plates. Previous studies suggested that nanostructure processed on culture dish affected cell adhesion [3, 4]. In the present study, nanostructure was processed on a silicon wafer using a nanoprocessing technology, and the structure was replicated to a polydimethylsiloxane (PDMS) plate. Human synovium-derived mesenchymal stem cells were cultured on the plate, and cell adhesion and morphological observation were performed.
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Boepple, Kathrin, Meng Dong, Emma Davis, Julia Schueler, Heike Walles, John Hickman, Walter E. Aulitzky, and Heiko van der Kuip. "Abstract 5040: Perfusion air culture of tissue slices: A new method to cultivate tumor tissue with minimal culture-dependent tissue stress." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-5040.
Ou, Ri-Ming, Fang-Hua Niu, Yu-Jie Yang, and Zhi-Hui Li. "The Tissue Culture Technique of Sloanea hemsleyana." In 2015 International Conference on Medicine and Biopharmaceutical. WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789814719810_0182.
van Vlimmeren, Marijke A. A., Anita Driessen-Mol, Cees W. J. Oomens, and Frank P. T. Baaijens. "The Potential of Prolonged Tissue Culture to Reduce Stress Generation and Retraction in Engineered Heart Valve Tissues." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53120.
Heart valve tissue engineering (TE) relies on extracellular matrix production by cells seeded into a degrading scaffold material. Valves are cultured constraint with the leaflets attached to each other for 4 weeks [1]. The seeded cells naturally exert traction forces to their surroundings and due to an imbalance between scaffold, tissue and these traction forces, stress is generated within the tissue, which is good for tissue formation and architecture. However, during culture it causes tissue compaction, resulting in leaflet flattening, and at time of implantation, the leaflets are separated and the generated stress causes retraction of the leaflets (fig 1). This retraction on its turn results in loss of functionality.
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Matsumoto, Takuya, and Seiji Aoyagi. "Removing mesenchymal cells from gland tissue on micro-patterned tissue culture dish." In 2012 International Symposium on Micro-NanoMechatronics and Human Science (MHS). IEEE, 2012. http://dx.doi.org/10.1109/mhs.2012.6492441.
Rolfe, P. "Metabolic measurements in cell culture and tissue constructs." In International Symposium on Instrumentation Science and Technology, edited by Jiubin Tan and Xianfang Wen. SPIE, 2008. http://dx.doi.org/10.1117/12.810606.
XIE, YAOJIAN. "ADVANCES IN TISSUE CULTURE OF EUCALYPTUS IN CHINA." In Proceedings of the International Symposium. WORLD SCIENTIFIC, 2003. http://dx.doi.org/10.1142/9789812704504_0011.
Scott, C. D., and D. K. Dougall. Plant cell tissue culture: A potential source of chemicals. Office of Scientific and Technical Information (OSTI), August 1987. http://dx.doi.org/10.2172/5938126.
Ostry, M. E., and K. T. Ward. Bibliography of Populus cell and tissue culture. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Forest Experiment Station, 1991. http://dx.doi.org/10.2737/nc-gtr-146.
Harris, David T. Tissue Culture Hood for Immunotoxicology of JP-8 Fuel. Fort Belvoir, VA: Defense Technical Information Center, July 2000. http://dx.doi.org/10.21236/ada383524.
Reisch, Bruce, Pinhas Spiegel-Roy, and Aliza Vardi. Tissue Culture and Gene Transfer for Genetic Improvement of Grapes. United States Department of Agriculture, November 1991. http://dx.doi.org/10.32747/1991.7599656.bard.
Peehl, Donna M. Development of a Novel Tissue slice Culture Model of Human Prostate Cancer. Fort Belvoir, VA: Defense Technical Information Center, February 2005. http://dx.doi.org/10.21236/ada435857.
Peehl, Donna M. Development of a Novel Tissue Slice Culture Model of Human Prostate Cancer. Fort Belvoir, VA: Defense Technical Information Center, February 2003. http://dx.doi.org/10.21236/ada417612.
Kraybill, William H. Lectin Enzyme Assay Detection of Viruses, Tissue Culture, and a Mycotoxin Simulant. Fort Belvoir, VA: Defense Technical Information Center, September 1988. http://dx.doi.org/10.21236/ada276469.
Peehl, Donna M. Development of a Novel Tissue Slice Culture Model of Human Prostate Cancer. Fort Belvoir, VA: Defense Technical Information Center, February 2004. http://dx.doi.org/10.21236/ada425981.
Labrune, Elsa, Bruno Salle, and Jacqueline Lornage. An update on in vitro folliculogenesis: a new technique for post-cancer fertility. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, August 2022. http://dx.doi.org/10.37766/inplasy2022.8.0111.
Review question / Objective: The present review intends to summarize the progress of in vitro folliculogenesis in humans. It focuses on the culture media and then, according to the culture stage, on the different culture systems developed with comments on the results obtained. Condition being studied: This review focuses on the progress of in vitro folliculogenesis in humans. Eligibility criteria: Inclusion criteria : all original English-language articles on in vitro folliculogenesis from ovarian tissue in humans; exclusion criteria: non-English papers, works on animals, in vitro maturation and in vivo maturation works carried out within the context of in vitro fertilization protocols, studies on in vitro folliculogenesis that checked slow freezing and/or vitrification of ovarian tissue, studies on frozen or vitrified tissues (these do not have the same objective), studies on short culture times, and studies that lacked major results.
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Peehl, Donna M. Discovery of Hyperpolarized Molecular Imaging Biomarkers in a Novel Prostate Tissue Slice Culture Model. Fort Belvoir, VA: Defense Technical Information Center, June 2013. http://dx.doi.org/10.21236/ada580953.
