Dissertations / Theses: 'Thy-1 glycoprotein'

1

Li, Lei. "The regulation of stanniocalcin-1 gene expression in rat sertoli and leydig cells." HKBU Institutional Repository, 2006. http://repository.hkbu.edu.hk/etd_ra/784.

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2

Kemp, Pauline Anne. "The glycosylation of human alpha-1-antitrypsin expressed in transgenic mouse milk." Thesis, University of Kent, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298167.

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3

Edmonds, Judith Helen Clinical School St Vincent's Hospital Faculty of Medicine UNSW. "Functional characterisation of the HIV-1 glycoprotein-41 cytoplasmic tail." Publisher:University of New South Wales. Clinical School - St Vincent's Hospital, 2009. http://handle.unsw.edu.au/1959.4/44099.

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The unusually long Cytoplasmic tail (CT) of Human Immunodeficiency Virus Type-1 (HIV-1) glycoprotein-41 (gp41) is highly conserved and engineered large truncations often render the virus non-infectious in a cell-type dependent manner. While large CT truncations occur infrequently in natural isolates, little is known about the mechanisms involved in infectious virions harbouring a large CT truncation. This thesis characterises RFgp34, a replication competent laboratory HIV-1 isolate with an acquired 100 amino acid CT truncation, and how it diverged from wildtype RF. The CT truncation and two possible compensatory mutations in Matrix (E40K and F44I) were introduced into the HIV-1 isolate NL4-3. These mutants were tested for infectivity, syncytia formation and glycoprotein incorporation into virions, alternative co-receptor usage and sensitivity to the fusion inhibitor T-20. Compared with RFwt, RFgp34-infected cultures displayed delayed viral replication kinetics in all cell types. Similar sized (MT-4 cells, PBMC) or larger and more numerous syncytia (Hut78 cells) were detected in RFgp34-infected cultures. Similar (Hut78 cells) or decreased (MT-4 cells, PBMC) amounts of glycoprotein was incorporated into RFgp34 virions, compared with RFwt virions. The increased syncytia in RFgp34-infected Hut78 cultures and the reduced glycoprotein incorporation into RFgp34 virions from MT-4 cells and PBMCs may explain the delayed RFgp34 replication kinetics. The Matrix E40K and F44I mutations were not able to directly compensate for the CT truncation to restore infectivity in Hut78 and MT-4 cells, as secondary mutations or the reversion of the CT truncation to a full-length CT were observed. In PBMCs the Matrix mutations alone were able to partially restore infectivity, suggesting specific mutations may compensate for the CT truncation in different cell types. None of the viruses utilised alternative HIV-1 co-receptors, nor were more resistant to T-20 than wildtype HIV-1 suggesting that the CT does not directly play a role in these viral functions. This thesis suggests that the sequence of mutations acquired by RFgp34 to compensate for the CT truncation and restore infectivity in multiple cell types may have occurred in a specific order and the evolution of RFgp34 to out-compete RFwt occurred over many passages.

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4

Bruce, Rachel S. "The structural heterogeneity of alpha-1-acid glycoprotein in asthma." Thesis, University of Strathclyde, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.401354.

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5

Stewart, Yvonne M. "The characterisation of alpha 1 acid glycoprotein in rheumatoid arthritis." Thesis, University of Strathclyde, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248709.

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6

Lai, Pei-Jen. "HIV-1 neutralisation and other aspects of the envelope glycoprotein." Thesis, Imperial College London, 2009. http://hdl.handle.net/10044/1/5472.

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The absence of an effective humoral response contributes to the failure of controlling HIV-1 infection. Methods to elicit a potent neutralising antibody response is still underway and this thesis explores three aspects that can affect neutralisation. The pseudovirus-based assay is now recommended as the standard assay for assessing neutralising antibody response. However, recent studies have reported discrepancies between pseduovirus-based assay and conventional PBMCs or other cell-based assays. The first aim is to investigate possible causes behind this difference. The effect of virus producer cell type and virus platform (pseudovirus vs. replication-competent) was examined and both parameters can affect neutralisation. The second aim was to study the the neutralising antibody response in patients with primary HIV-1 infection. A panel of 6 patients were selected from the SPARTAC clinical trial. Pseudoviruses were constructed with the env gene derived from patient samples at week 0 (baseline) and week 52. The evolutionary history and the neutralisation sensitivity of these primary isolates against a panel of broadly neutralising antibodies and heterologous and autologous sera were studied. The week 52 isolates escaped from antisera neutralisation rapidly, most likely at the glycan level. In addition, viral load was found to correlate directly with intra-patient viral diversity and evolutionary divergence over time. Finally, the effect of Nef on HIV-1 neutralisation was investigated. Recent reports suggest that Nef modifies HIV-1 envelope glycoprotein. Hence, neutralising antibody response might also be modified in the absence of Nef. When subjected to neutralisation with a panel of monoclonal antibodies, the Nef-deleted (ΔNef) HIV-1 was more readily neutralised than the wild-type (WT) virus. Immunoprecipitation assays showed that neutralising antibodies can capture ΔNef virus more efficiently than WT virus. However, the enhanced neutralisation of ΔNef virus was neither due to CD4-down regulation nor difference in glycosylation.

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7

Anwar, Mohammad Arif. "Analysis of the fowlpox virus homologue of mammalian PC-1 glycoprotein." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321939.

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8

Ueno, Shohta. "Functional analysis of the herpes simplex virus type-1 glycoprotein H." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608640.

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9

Cardy, Caroline Maria. "The structure and function of calcium binding epidermal growth factor-like domains in human fibrillin-1." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360210.

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10

Sheppard, Neil Christopher. "The serology of HIV-1 envelope glycoproteins for vaccine use." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.436954.

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11

Gardner, Matthew Ryan. "Targeting the CD4- and Coreceptor-Binding Sites of the HIV-1 Envelope Glycoprotein." Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11644.

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The HIV-1 envelope glycoprotein, Env, facilitates the translocation of the viral capsid across the cellular membrane. Env is a trimer of hetero-dimers composed of a gp120 subunit and gp41 transmembrane protein. The gp120 subunit binds the primary receptor, CD4, leading to conformational changes of Env that then promote binding to the coreceptor, principally CCR5 or CXCR4. As the sole protein on the surface of the virion, Env is under continuous pressure from the host's antibody response. Two classes of antibodies target the highly conserved receptor-binding sites of gp120: CD4-binding site (CD4bs) and CD4-induced (CD4i) antibodies.

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12

Reske, A. "The innate immune response to HSV-1 : glycoprotein mediated activation of dendritic cells." Thesis, University College London (University of London), 2009. http://discovery.ucl.ac.uk/15835/.

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Herpes Simplex Virus (HSV) – 1 also known as Human Herpes Virus (HHV) – 1 is a common infectious agent of humans which can cause a wide variety of clinical outcomes, ranging from mild mucocutaneous lesions to long term morbidity and possible mortality. Viral entry into cells requires the co-ordinated action of at least four HSV-1 envelope glycoproteins: gB, gD and the heterodimer gHgL. Dendritic cells (DC) are the most potent antigen presenting cells and are likely to encounter the virus early after entry into the host. HSV-1 readily infects DC leading to a series of both morphological and functional changes, and yet the pathway induced by HSV-1 that leads to DC maturation has not been elucidated. This thesis aims to understand the role of the viral entry glycoproteins in the activation of DC and the consequent initiation of an immune response. Defining this role has important implications not only in understanding immunopathogenesis, but also in the study of HSV-1 as an immunotherapeutic vector, and in the design of an efficient HSV-1 vaccine. Monocyte-derived DCs (MDDC) were found to recognise and respond to the complex of four essential viral glycoproteins, independent of other viral proteins or nucleic acids. MDDC recognition of these four glycoproteins leads to the upregulation of a maturation phenotype and the production of type I interferon (IFN) as well as the induction of a strongly polarised TH1, IFN-γ dominated allogeneic T cell response. In contrast, monocyte-derived Langerhans cells (MDLC), display only partial maturation phenotype and do not produce type I IFN. Plasmacytoid DC (pDC) induce a strong type I IFN response to a viral infection, but not to the viral surface glycoproteins. In the context of natural HSV-1 infection, these results suggest a model in which at the site of HSV-1 infection, different DC sub-populations respond differently to a viral infection and to glycoproteins expressed on the surface of infected cells in order to provide an effective immune response.

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13

Yule, Fiona Sara Bridget. "The correlation between the glycosylation of Alpha-1-acid glycoprotein and healing in wounds." Thesis, University of Strathclyde, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366836.

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14

Martimbeau, Stephanie. "Receptor-mediated endocytosis of testicular sulfated glycoprotein-1 (SGP-1) by the nonciliated cells of the rat ductuli efferentes." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22770.

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The present study examines the endocytosis of testicular sulfated glycoprotein-1 (SGP-1) by the nonciliated cells of the efferent ducts. SGP-1 is a 70 KDa protein secreted by the Sertoli cells. Once secreted in the seminiferous lumen, the protein binds to the tail of spermatozoa. In the efferent ducts, it is endocytosed by the nonciliated cells, presumably via a receptor-mediated process. Because the initial steps of receptor-mediated endocytosis result from the binding of a ligand's terminal oligosaccharide to a receptor on the ceil surface, several monosaccharides were injected into the lumen of the rete testis to study their effect on the endocytosis of SGP-1 in the efferent duct. The labeling density of various endocytic compartments was estimated and compared in untreated and treated animals with various sugars. The following sugars were tested: glucose, galactose, mannose, mannose 6-phosphate, N-acetylglucosamine, N-acetylgalactosamine, and sialic acid. The findings suggest that, via its sialic acid binding domain, SGP-1 may bind to glycolipids on the tail of spermatozoa, remove them from the membrane forming a lipo-protein complex. The complex may then be endocytosed by the nonciliated cells, via a receptor that would recognize SGP-1's terminal sialic acid residues, and be delivered to the lysosomes to be degraded. (Abstract shortened by UMI.)

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15

Zhou, Amy Yuan. "Exploring the functional role of herpes simplex virus type-1 glycoprotein H in virus entry." Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648658.

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16

Chadwick, James Steward. "The identification of β-lymphocyte epitopes of herpes simplex virus type 1 glycoprotein H." Thesis, University of Leicester, 1991. http://hdl.handle.net/2381/35320.

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Herpes simplex virus type 1 glycoprotein H is a minor viral constituent which has been implicated in viral mechanisms of cell entry and exit. Study of this molecule has been hindered by a relative lack of specific immunological reagents. This study reports the generation and characterisation of specific polyclonal antibodies. The purification of gH and the generation of polyclonal serum is described. Antigenic determinants reactive with polyclonal antibodies were determined using synthetic peptides derived from the primary amino acid sequence of the molecule and a similar aproach was applied using available monoclonal antibodies. A series of 833 hexapeptides, with five residue overlap, were synthesised representing all potential continuous epitopes comprised of six residues or less. Peptides, displaying structural homology with epitopes of the native molecule were identified by screening for antibody binding. To further validate the identity of epitopes, corresponding homologous peptides were conjugated to carrier molecules and used to generate anti-peptide antibodies. The biological and immunological properties of the resulting anti-peptide antibodies were determined. Three antipeptide antibodies appeared reactive with the glycoprotein either following western transfer or in immunoprecipitation confirming the identity of gH-1 epitopes.

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17

Parikh, Khyati. "Investigating the role of auto-immune responses to transient axonal glycoprotein-1 (TAG-1) in experimental autoimmune encephalomyelitis (EAE)." Thesis, Available from the University of Aberdeen Library and Historic Collections Digital Resources, 2009. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=25214.

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18

Hay, Nina. "Alternative splicing and tissue distribution of the mouse sulfated glycoprotein-1 (SGP-1/prosaposin) mRNA and its translation product." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq29713.pdf.

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19

Rodger, Gaener. "A study of the interactions between the glycoproteins of herpes simplex virus type-1." Thesis, University of Cambridge, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624801.

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20

Griffiths, Caroline Mary. "The cloning and expression of Herpes simplex virus type 1 glycoprotein C in vaccinia virus." Thesis, University of Leicester, 1991. http://hdl.handle.net/2381/35382.

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The Herpesviridae family contains numerous virus types, several of which can infect man. The virus which is most widely spread in terms of infection is herpes simplex virus (HSV), of which there are two serotypes (HSV-1 and HSV-2). Following a primary infection, the virus can establish a latent infection within neurons of sensory ganglia, where it remains throughout the lifetime of the host. HSV is able to reactivate from the latent state, and may produce clinical infection at the site of the initial virus invasion (recrudescent lesions). Although most HSV-1 infections are mild or subclinical, infection can lead to life-threatening illness. The search for an effective HSV vaccine has met with limited success to date. In recent years, vaccine research has turned toward the use of viral vectors, for the expression of individual virus proteins with a view to stimulating host immunity. A great deal of attention has been focussed on the vaccinia virus, which is able to accept large amounts of foreign DNA into it's genome with no loss of viability. Several HSV proteins have been expressed from recombinant vaccinia viruses, and this project outlines the cloning of DNA sequences encoding the HSV-1 glycoprotein C, and expression of that protein from a recombinant vaccinia virus. The HSV DNA sequences encoding the glycoprotein were cloned from an existing library, into plasmid vector pSC11. This plasmid allows insertion of gC sequences into the vaccinia virus genome by way of homologous recombination. The plasmid also provides a vaccinia virus promoter for the control of gC transcription during infection of cells with the resulting recombinant viruses. Recombinant vaccinia viruses produced on cloning of a 1.75kb NheI/SphI fragment containing the HSV-1 gC gene did not express the glycoprotein during infections. Vaccinia virus DNA polymerase is known to be sensitive to secondary structures within DNA, and the 5' non-coding region of the HSV gC gene is rich in GC basepairs, which could readily form such structures. Site-directed mutagenesis removed the 5' non-coding region (34 nucleotides) in an attempt to remove any such block to gC transcription. Vaccinia viruses containing the mutated sequences were able to express gC within infected cells, as determined by immunofluorescence. Mice inoculated with a gC-expressing recombinant vaccinia virus were able to induce HSV-neutralising antibodies and displayed 50% protection against a lethal HSV-1 challenge. The implications of gC-expressing vaccinia recombinant viruses, with respect to the search for an HSV vaccine and to the understanding of the role of gC during HSV infections is discussed.

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21

French, Deborah. "The correlation between alpha-1-acid glycoprotein glycosylation and collagen fibril formation in burns' injury." Thesis, University of Strathclyde, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269895.

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22

Forrester, Audrey Jane. "Studies on the function and antigenicity of glycoprotein H of Herpes simplex virus type 1." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386055.

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23

Behan, Jennifer L. "The binding ability of alpha-1-acid glycoprotein as a mechanism of resistance to methadone." Thesis, Edinburgh Napier University, 2010. http://researchrepository.napier.ac.uk/Output/4446.

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Dependence on heroin and other opioids represents a considerable problem worldwide. There is a continual need to improve therapy and/ or find more efficacious alternatives if these issues are to be addressed. The most commonly implemented pharmacological therapy in treating said dependencies is methadone; however its success is the subject of ongoing debate. Certain plasma proteins including alpha1-acid glycoprotein (AGP) bind to drugs which causes inactivation and, if low enough, may prevent a therapeutic effect being attained. The hepatic synthesis of AGP increases two- to five-fold during numerous physiological and pathophysiological conditions, becoming the most prevalent acute phase protein in the blood. Additionally, the structure of the sugar chains (glycans) attached to the surface of underlying polypeptide backbones can differ, potentially altering the functions performed. AGP was isolated from blood samples obtained from patients undergoing various stages and types of opioid-replacement therapy and from heparinised blood samples provided by the Blood Transfusion Service. Structural analysis of the glycans was undertaken primarily through the use of high pH anion-exchange chromatography (HPAEC) and intrinsic fluorescence used as a measure of drug binding. The composition of glycans attached to the polypeptide backbone of AGP isolated from patient samples was found to markedly differ from that of a ‘normal' healthy population. Levels of galactose and N-acetyl-glucosamine were amplified in all methadone treatment groups which suggested increased branching of glycans; this was supported by HPAEC analysis of complete glycan chains. Binding of methadone to all isolated AGP samples was elevated at the highest drug concentrations tested; however the degree of quenching appeared to be greater in patients. Therefore, the glycoforms expressed by AGP appear to be associated with the subsequent binding of the glycoprotein to methadone. It is possible that altered glycosylation could increase affinity for the drug, reducing its bioactive concentration to below that required to produce the pharmacological effect. Currently, the doses of methadone used in opioid replacement therapy are primarily influenced by the expression of physical symptoms, however this preliminary study has indicated that determination of the level and glycoform expression of AGP may offer potential use when determining the most effective therapy and dosage regimen.

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24

Thormann, Nora Mariella [Verfasser]. "The role of the secreted glycoprotein G (gG) in equine herpesvirus type 1 (EHV-1) immune modulation and virulence / Nora Mariella Thormann." Berlin : Freie Universität Berlin, 2012. http://d-nb.info/1026991765/34.

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25

Rosenthal, Allison Lianne. "Sulfated glycoprotein-1 (SGP-1) biosynthesis and its regulation within the nonciliated cells of rat efferent ducts : an in vivo study." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68250.

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The nonciliated epithelial cells of the efferent ducts are specialized in internalizing luminal fluid. They possess an extensive endocytic apparatus which provides an ideal system to study the kinetics of endocytosis. The nonciliated cells actively endocytose sulfated glycoprotein-1 (SGP-1, 70 kDa), a major secretory protein of Sertoli cells. A second form of SGP-1 (65 kDa) present in the secondary lysosomes of Sertoli and nonciliated cells of the efferent ducts is believed to be the equivalent of human prosaposin, the precursor of four small heat stable proteins (saposins A, B, C and D) required for the hydrolysis of sphingolipids by specific lysosomal hydrolases. This study was undertaken to investigate the contribution of Sertoli-derived SGP-1 as well as a putative endogenous SGP-1 within the secondary lysosomes of nonciliated cells. The hormonal control of endogenous lysosomal SGP-1, specifically the influence of testosterone withdrawal and its subsequent replacement was also examined. The results provide evidence that the nonciliated cells of the efferent ducts are involved in the clearance of testicular SGP-1 and reveal the presence of 15 kDa saposins and their 65 kDa precursor in secondary lysosomes of these cells. In addition, the production of an endogenous lysosomal SGP-1 targeted from the Golgi apparatus to the lysosomes after its glycosylation has also been demonstrated. The results further reveal that the endocytosis and lysosomal targeting of SGP-1 in nonciliated cells of the efferent ducts appear to be pituitary controlled.

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26

Boneham, Steven Paul. "Enrichment of random peptide display libraries by antibodies to the HIV-1 envelope glycoproteins." Thesis, University of Nottingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342490.

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27

Sullivan, Veronica. "Analysis of the herpes simplex virus type 1 glycoproteins using recombinant vaccinia virus vectors." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292957.

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28

Pike, Andrew. "Effects of P-glycoprotein on brain penetration of drugs in the CF-1 mdrla -/- mouse model." Thesis, King's College London (University of London), 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414744.

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29

Jenette, Kankanamge Pushpa. "Studies on the Epitope of Rabies Virus Glycoprotein Recognized by a Monoclonal Antibody #1-30-44." 京都大学 (Kyoto University), 2003. http://hdl.handle.net/2433/149174.

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30

Khati, Makobetsa. "Macrophage-HIV interactions : aptamers against the gp120 surface envelope glycoprotein of the macrophage tropic strains of HIV-1." Thesis, University of Oxford, 2002. http://ora.ox.ac.uk/objects/uuid:a32becf2-bf5d-4428-b598-e8057d977fbd.

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HIV-1 has evolved a number of strategies in response to current anti-retroviral drugs and the selection pressure of humoral and cellular immunity. In particular, R5 viral strains that are essential for AIDS pathogenesis are very resistant to neutralization by antibodies. Therefore, the aim of this thesis was to develop synthetic nucleic acid ligands, aptamers, against gp120 of an R5 strain of HIV-1, with a view of using aptamers as novel neutralization molecules and analytical tools to study HIV-1 entry into target cells. The central hypothesis of this thesis was that aptamers by virtue of their small size and slow dissociation rates, compared to antibodies, would easily access and bind occluded gp120 neutralization sites. Using the SELEX protocol and SPR technology, I isolated 2'-Fluoro-pyrimidine-RNA aptamers against HIV-l_Ba-L monomeric gp120. Most of these aptamers not only bound gp120 with high affinities but also neutralized R5 primary isolates in human PBMC by 1,000 to 100,000-fold, truly unprecedented when compared with natural ligands such as antibodies. Some aptamers, like B4, defined a conserved site of gp120 that could not mutate to escape neutralization following stringent selection, in vitro, for breakthrough virus. This was consistent with subsequent findings that B4 aptatope (binding site) overlaps a poorly immunogenic but highly conserved CD4-induced epitope as determined by competition with 17b and 48d mAbs that map to this neutralization epitope on the gp120. This study was thus the first of its kind to describe neutralization of HIV-1 primary isolates by a ligand against the CD4-induced epitope. Most intriguing, although B4 potently neutralized HIV-1_Ba-L infection in PBMC, which is a mixed T cell and macrophage population, it modestly neutralized infection of the same virus in a purified culture of macrophages. These findings are intriguing in that they suggest that aptamers could be used to dissect unique sites on the virus that interact with target cell surface in ways that have not been revealed heretofore, and would help understand better HIV-1 entry pathways, especially in macrophages. Thus neutralizing aptamers such as these could be exploited to provide leads in developing alternative anti-HIV-1 drugs and a deeper understanding of the molecular interactions between the virus and its host cell.

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Harman, A. "Functional analysis of the transmembrane domain and cytoplasmic tail of Herpes simplex virus type-1 glycoprotein H." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603719.

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Recently a role for the transmembrane (TM) domain and cytoplasmic tail in mediating membrane fusion has been demonstrated in many virus fusion proteins and therefore the importance of these regions of HSV-1 gH in this process was investigated. Chimeric constructs were generated in which the TM domain and/or cytoplasmic tail of gH were replaced with analogous regions from other proteins and these constructs were characterised using two assays. Firstly a transient transfection cell fusion assay was used in which cells expressing HSV fusion proteins form syncytia with neighbouring untransfected cells. Secondly a complementation assay was used which measures the ability of these constructs to rescue the infectivity of a gH null virus. These experiments demonstrated the importance of both these regions of gH in mediating membrane fusion as judged by either assay. Specific sequence requirements within these regions were then examined by constructing a series of deletion, truncation or substitution mutants that were then tested using the same assay methods. The results confirmed and extended previous studies which had implicated an SVP motif in the cytoplasmic tail in membrane fusion, and identified key residues in the transmembrane domain (notably a central glycine residue) that are required for fusion function. No gH mutants were isolated that were functional in the cell fusion assay, yet failed to function in virus rescue assays. This implies that neither the cytoplasmic domain nor the transmembrane domain contains specific sequences required for assembly into the virus envelope. This was confirmed by showing that chimeric molecules in which both the TM and cytoplasmic tail domains of gH were replaced by the equivalent domains of the cell-surface protein CD8, were incorporated into virions as efficiently as a wild type gH molecule. It is unclear how gH molecules are directed into the virion envelope. Many mutants that were apparently non-functional in cell fusion were, nevertheless, capable of rescuing virus infectivity. More detailed analysis demonstrated, however, that in these instances the rescued virions entered cells much more slowly than normal virions and that the behaviour of mutant molecules in the two assays was generally consistent. It is apparent that rescue assays which merely record recovery of infectious virus can be misleading.

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32

Anderson, Nicola Elizabeth. "The use of subtle variations in alpha-1-acid glycoprotein glycosylation to distinguish between specific liver diseases." Thesis, University of Strathclyde, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273446.

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33

Hay, Nina. "Alternative splicing and tissue distribution of the mouse sulfated glycoprotein-1 (SGP-1prosaposin) mRNA and its translation product." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27338.

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The alternative splicing of the prosaposin gene results in the inclusion or exclusion of a 9 bp insertion of exon 8, which is located in the saposin B domain of this gene. Thus, exon 8 codes for three amino acid residues (Gin-Asp-Gln), which may potentially be present or absent in human prosaposin. Recently, it was shown that the alternative splicing of the prosaposin gene may be tissue specific and that a possible function of the three amino acid insertion could be to alter the binding specificity of saposin B towards different glycosphingolipids. We have recently cloned the murine SGP-1 gene and found that it also contains the 9 bp exon 8. In the present study we have used reverse transcription-polymerase chain reaction (RT-PCR) to study the distribution of the alternatively spliced mRNAs in several tissues. We have also used Northern Blot analysis to confirm the expression and stability of these transcripts, and light microscope immunocytochemistry to examine whether or not alternative splicing affects the translation of the mRNA transcripts into mature proteins. (Abstract shortened by UMI.)

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34

Paterson, Sarah C. "An investigation of the glycosylation and drug binding ability of alpha-1-acid glycoprotein in chronic myeloid leukaemia." Thesis, University of Strathclyde, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.415366.

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35

Chang, Eddie. "The role of perforin and chemokines in the pathogenesis of chronic corneal inflammation induced by herpes simplex virus type-1 infection." free to MU Campus, others may purchase, 2003. http://wwwlib.umi.com/cr/mo/fullcit?p3091911.

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36

MacLeod, Iain James. "A role for the binding of Herpes Simplex Virus Type-1 glycoproteins in the induction of intracellular signalling." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612092.

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37

Postler, Thomas Verfasser], and Bernhard [Akademischer Betreuer] [Fleckenstein. "The Cytoplasmic Domain of the HIV-1 and SIV Envelope Glycoprotein: Functional Properties and Topology / Thomas Postler. Betreuer: Bernhard Fleckenstein." Erlangen : Universitätsbibliothek der Universität Erlangen-Nürnberg, 2012. http://d-nb.info/1024198774/34.

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38

Johnson, Deborah-Ann. "Disease specific glycosylation changes in the stucture of alpha-1-acid glycoprotein may influence the binding affinity to tuberculosis drugs." Thesis, University of Strathclyde, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.435112.

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39

Li, Yuanhao. "Structural and functional study of bovine herpesvirus 1 glycoprotein B in the interaction with Madin Darby bovine kidney cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24030.pdf.

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40

Copeland, Ronald Jarrod Liu Jian. "The preparation and characterization of a heparin-derived oligosaccharide that binds to herpes simplex virus type 1 glycoprotein D." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2006. http://dc.lib.unc.edu/u?/etd,704.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2006.
Title from electronic title page (viewed Oct. 10, 2007). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the School of Pharmacy (Medicinal Chemistry and Natural Products)." Discipline: Medicinal Chemistry and Natural Products; Department/School: Pharmacy.

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Dey, Antu Kanti. "Structural characterisation of RNA aptamers against the gp 120 surface envelope glycoprotein of a macrophage tropic strain of HIV-1." Thesis, University of Oxford, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409036.

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Mooney, Paul Joseph. "The use of alpha-1-acid glycoprotein as a putative non-invasive marker of fibrosis and prognostic indicator of cirrhosis." Thesis, University of Strathclyde, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.426354.

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Mohan, Hosahalli Krishnamurthy. "Inhaled 99mTc-sestamibi clearance from lungs and its relation to the expression of P-glycoprotein and Multidrug Resistance Protein-1." Thesis, King's College London (University of London), 2016. https://kclpure.kcl.ac.uk/portal/en/theses/inhaled-99mtcsestamibi-clearance-from-lungs-and-its-relation-to-the-expression-of-pglycoprotein-and-multidrug-resistance-protein1(d5e7396e-8448-4f5c-ac7c-aaea224987a7).html.

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P-glycoprotein (P-gp) and Multidrug Resistance Protein (MRP1) are the key cellular drug efflux transporters in a range of tissues and the radiopharmaceutical 99mTc-sestamibi has been demonstrated to be a substrate for both transporters in a number of in vivo and in vitro studies. The elimination rate of 99mTc-sestamibi from the lungs following inhalation as an aerosol has been shown to be delayed in healthy smokers versus non-smokers. It was hypothesized that this was the result of smoke-induced up-regulation of P-gp. The aims of this study were: To examine the relationship between the lung elimination rate of inhaled sestamibi and immuno-histochemical expression of broncho-pulmonary MRP1 and P-gp. To study the repeatability of the technique and assess the effect of various demographic factors affecting the clearance of inhaled sestamibi. Methods: Thirty-five participants were included in the study. They all underwent an inhaled 99mTc-sestamibi clearance study. Of these, 13 were patients undergoing surgery for primary lung cancer (5/13) or spontaneous pneumothorax (8/13). There were 22 healthy volunteers. All participants gave informed consent prior to the study. Semi-quantitative assessment (grade 0-3) of MRP1 and P-gp expression in lung on immuno-histochemical examination was correlated with the elimination rate of sestamibi from the lung tissue. The effect of various factors on inhaled sestamibi clearance including smoking status, age, gender and locality of residence was evaluated. The study was repeated in 10 participants to assess the repeatability and inter-observer reproducibility of the technique. Results: MRP1 expression was seen in 12/13 patients while P-gp in only 2/13. The mean sestamibi elimination rate was faster in patients expressing low levels of MRP1 expression (grade 0-1), mean T½ of 105 ± 20 min, compared with those with higher levels of MRP1 expression (grade 2-3), mean T½ of 149 ± 28 min (P = 0.008). Inhaled sestamibi clearance was significantly delayed in smokers (n=17), mean T½ = 142 ± 29 min, compared to non smokers (n=18), mean T½ = 91 ± 14 min (P < 0.0001). The clearance of inhaled sestamibi was also significantly delayed in non smoking older patients >30 y (n= 6), mean T½ = 101 ± 15 min, compared to younger patients <30 y (n=12), mean T½ = 86 ± 11 min (P < 0.05), and in non smoking men (n=7), T½ = 130 ± 37 min, compared to women (n=11), T½ = 105 ± 28 min (P < 0.05). There was however no significant difference noted between urban dwellers from London (n=22), mean T½ = 89 ± 11 min, compared to semi-rural dwellers (n=13), mean T½ = 96 ± 20 min (P > 0.05). Bland-Altman analysis revealed excellent agreement between test and re-test values with a (bias) of 4.8 min and a standard deviation of the difference (precision) of 5.7 min. There was an excellent inter-observer reproducibility seen with a Spearman Rho value of 0.96 and p<0.05 Conclusion: The study results indicate a significant correlation between the predominant transporter in lung (broncho-alveolar epithelium) MRP1 and inhaled 99mTc- Sestamibi clearance. Inhaled 99mTc- Sestamibi clearance is a safe and reproducible technique. Smoking, increasing age and male gender appear to significantly prolong the lung clearance of inhaled sestamibi. There was however no definite effect of environmental status on the clearance rate observed.

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Stumpe, Marion. "The development of methods for the determination of unbound local anaesthetics and alpha-1 acid glycoprotein in small volumes of neonatal plasma." Thesis, University of Strathclyde, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248366.

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45

Gantner, Benjamin N. "Dectin-1 and toll-like receptor 2 : recognition of pathogens through multiple receptors shapes the innate immune response /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/8346.

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46

Pham-Hung, d'Alexandry d'Orengiani Anne-Laure. "The accessory glycoprotein gp3 of canine Coronavirus type 1 : investigations of sequence variability in feline host and of the basic features of the different variants." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA114831/document.

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Les différents génotypes de Coronavirus canins (CCoV-I/II) et félins (FCoV-I/II) sont phylogénétiquement proches, suggérant des transmissions inter-espèces entre chiens et chats. Lors d’analyses de séquences menées sur des chats infectés, des souches félines atypiques ont pu être mises en évidence, contenant un gène S de type FCoV-I, un gène N de type CCoV-I, ainsi que la présence du gène ORF3, spécifique à CCoV-I. Dans ces souches, le gène ORF3 est présent avec une ou deux délétions toujours identiques, conduisant à la synthèse de protéines tronquées gp3-Δ1 et gp3-Δ2. Les délétions de protéines accessoires étant déjà impliquées dans les transmissions inter-espèces, une étude de caractérisation de la protéine gp3 et de ses différentes formes a été menée. Les trois protéines s’oligomérisent de manière covalente et sont retenues dans le réticulum endoplasmique, en absence de signal spécifique de rétention. Les délétions influencent le niveau d’expression des protéines en cellules félines, où seule l’expression de gp3-Δ1 est visible, alors qu’elles conservent toutes une expression optimale en cellules canines. En l’absence de souches de Coronavirus cultivables en laboratoire contenant le gène ORF3, des cellules canines exprimant l’une des protéines gp3 ont été infectées par une souche CCoV-II. Dans ce modèle, les protéines gp3 ne modifient pas le cycle viral. Dans un contexte d’émergence de nouveaux Coronavirus, la compréhension des mécanismes moléculaires de changement d’hôte est cruciale et les Coronavirus félins et canins peuvent représenter un modèle d’étude utile
The different genotypes of canine (CCoV-I/II) and feline (FCoV-I/II) Coronaviruses share a close phylogenetic relationship, suggesting inter-species transmissions between cats and dogs. Through sequence analyses of cat samples, atypical FCoV strains, harbouring an S gene related to FCoV-I, an N gene close to the CCoV-I cluster and the ORF3 gene, peculiar to CCoV-I, were discovered. This ORF3 gene was systematically truncated in feline samples, displaying either one or two identical deletions, leading to the translation of gp3-Δ1 and gp3-Δ2. As deletions in accessory proteins have already been involved in host-switch, studies of the different variants of gp3 were conducted. Results demonstrate that all proteins oligomerize through covalent bonds and are retained in the ER, without any specific retention signal. Deletions influence the expression level with a proper expression of the three proteins in canine cells, whereas only gp3-Δ1 expression is sustained in feline cells. As no isolates of Coronavirus harbouring the ORF3 gene exists, cells expressing the different gp3 proteins have been infected with a CCoV-II strain. In this model, the gp3 proteins do not influence the viral life cycle. In the light of emergence of new Coronaviruses, investigations on their molecular mechanisms during the host-switch are crucial and canine and feline Coronaviruses could represent a useful model

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Schwarzer, Roland [Verfasser], Andreas [Akademischer Betreuer] Herrmann, Martin [Akademischer Betreuer] Hof, and Michael [Akademischer Betreuer] Veit. "CRACking the Riddle : inquiring the role of the c holesterol binding motif of the HIV - 1 glycoprotein gp41 / Roland Schwarzer. Gutachter: Andreas Herrmann ; Martin Hof ; Michael Veit." Berlin : Humboldt Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2014. http://d-nb.info/1054989559/34.

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Yolamanova, Maral [Verfasser]. "A small peptide derived from the HIV-1 gp120 glycoprotein forms positively charged fibrils that enhance transduction efficiencies of retro- and lentiviral vectors. / Maral Yolamanova." Ulm : Universität Ulm, 2016. http://d-nb.info/1114272647/34.

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Majumdar, Avijit. "Regulation of the activation and activity of the extra-cellular signal regulated kinases 1 & 2 MAP kinase pathway by eukaryotic initiation factor 2 associated glycoprotein p67." [Kent, Ohio] : Kent State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=kent1209000031.

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Thesis (Ph.D.)--Kent State University, 2008.
Title from PDF t.p. (viewed Jan. 26, 2010). Advisor: Bansidhar Datta. Keywords: p67, ERK1, ERK2, oncogenic KRasV12, tumor suppressor. Includes bibliographical references (p. 143-160).

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50

Fulcher, Jennifer Ann. "Novel galectin-1 functions at the host-pathogen interface interactions with Nipah virus envelope glycoproteins and multifunctional roles in dendritic cell activation and development /." Diss., Restricted to subscribing institutions, 2008. http://proquest.umi.com/pqdweb?did=1680017811&sid=14&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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Dissertations / Theses on the topic 'Thy-1 glycoprotein'

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