Log in

Relevant bibliographies by topics / Thermoacidophiles / Journal articles

Journal articles on the topic 'Thermoacidophiles'

To see the other types of publications on this topic, follow the link: Thermoacidophiles.

Author: Grafiati

Published: 10 December 2022

Last updated: 28 January 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Thermoacidophiles.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Vetter, Anna M., Julia Helmecke, Dietmar Schomburg, and Meina Neumann-Schaal. "The Impact of Pyroglutamate:Sulfolobus acidocaldariusHas a Growth Advantage overSaccharolobus solfataricusin Glutamate-Containing Media." Archaea 2019 (April 24, 2019): 1–9. http://dx.doi.org/10.1155/2019/3208051.

Full text

Abstract:

Microorganisms are well adapted to their habitat but are partially sensitive to toxic metabolites or abiotic compounds secreted by other organisms or chemically formed under the respective environmental conditions. Thermoacidophiles are challenged by pyroglutamate, a lactam that is spontaneously formed by cyclization of glutamate under aerobic thermoacidophilic conditions. It is known that growth of the thermoacidophilic crenarchaeonSaccharolobus solfataricus(formerlySulfolobus solfataricus) is completely inhibited by pyroglutamate. In the present study, we investigated the effect of pyroglutamate on the growth ofS. solfataricusand the closely related crenarchaeonSulfolobus acidocaldarius.In contrast toS. solfataricus,S. acidocaldariuswas successfully cultivated with pyroglutamate as a sole carbon source. Bioinformatical analyses showed that both members of theSulfolobaceaehave at least one candidate for a 5-oxoprolinase, which catalyses the ATP-dependent conversion of pyroglutamate to glutamate. InS. solfataricus, we observed the intracellular accumulation of pyroglutamate and crude cell extract assays showed a less effective degradation of pyroglutamate. Apparently,S. acidocaldariusseems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared toS. solfataricus. Concludingly,S. acidocaldariusexhibits a more efficient utilization of pyroglutamate and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures.

APA, Harvard, Vancouver, ISO, and other styles

2

Colman, Daniel R., Saroj Poudel, Trinity L. Hamilton, Jeff R. Havig, Matthew J. Selensky, Everett L. Shock, and Eric S. Boyd. "Geobiological feedbacks and the evolution of thermoacidophiles." ISME Journal 12, no. 1 (October 13, 2017): 225–36. http://dx.doi.org/10.1038/ismej.2017.162.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Müller, Hans-Dieter, and Helmut Görisch. "Archaebacterial Citrate Synthases: The Enzymes from the Thermoacidophiles Sulfolobus acidocaldarius and Thermoplasma acidophilum Show pro-S Stereospecificity." Zeitschrift für Naturforschung C 44, no. 11-12 (December 1, 1989): 927–30. http://dx.doi.org/10.1515/znc-1989-11-1209.

Full text

Abstract:

Abstract Citrate synthase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius was purified 365-fold to electrophoretic homogeneity. At 40 °C and pH 8.1 the homogeneous enzyme shows a specific activity of 73 units per mg, which corresponds to a turnover number of 44 sec-1. Citrate synthase from S. acidocaldarius shows pro-S stereospecificity, as is found with a partially purified preparation of the enzyme from Thermoplasma acidophilum, another thermoacidophilic archaebacterium.

APA, Harvard, Vancouver, ISO, and other styles

4

Chaban, Bonnie, Sandy Y. M. Ng, and Ken F. Jarrell. "Archaeal habitats — from the extreme to the ordinary." Canadian Journal of Microbiology 52, no. 2 (February 1, 2006): 73–116. http://dx.doi.org/10.1139/w05-147.

Full text

Abstract:

The domain Archaea represents a third line of evolutionary descent, separate from Bacteria and Eucarya. Initial studies seemed to limit archaea to various extreme environments. These included habitats at the extreme limits that allow life on earth, in terms of temperature, pH, salinity, and anaerobiosis, which were the homes to hyper thermo philes, extreme (thermo)acidophiles, extreme halophiles, and methanogens. Typical environments from which pure cultures of archaeal species have been isolated include hot springs, hydrothermal vents, solfataras, salt lakes, soda lakes, sewage digesters, and the rumen. Within the past two decades, the use of molecular techniques, including PCR-based amplification of 16S rRNA genes, has allowed a culture-independent assessment of microbial diversity. Remarkably, such techniques have indicated a wide distribution of mostly uncultured archaea in normal habitats, such as ocean waters, lake waters, and soil. This review discusses organisms from the domain Archaea in the context of the environments where they have been isolated or detected. For organizational purposes, the domain has been separated into the traditional groups of methanogens, extreme halophiles, thermoacidophiles, and hyperthermophiles, as well as the uncultured archaea detected by molecular means. Where possible, we have correlated known energy-yielding reactions and carbon sources of the archaeal types with available data on potential carbon sources and electron donors and acceptors present in the environments. From the broad distribution, metabolic diversity, and sheer numbers of archaea in environments from the extreme to the ordinary, the roles that the Archaea play in the ecosystems have been grossly underestimated and are worthy of much greater scrutiny.Key words: Archaea, methanogen, extreme halophile, hyperthermophile, thermoacidophile, uncultured archaea, habitats.

APA, Harvard, Vancouver, ISO, and other styles

5

Shah, Shiraz Ali, Niels R. Hansen, and Roger A. Garrett. "Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism." Biochemical Society Transactions 37, no. 1 (January 20, 2009): 23–28. http://dx.doi.org/10.1042/bst0370023.

Full text

Abstract:

Transcripts from spacer sequences within chromosomal repeat clusters [CRISPRs (clusters of regularly interspaced palindromic repeats)] from archaea have been implicated in inhibiting or regulating the propagation of archaeal viruses and plasmids. For the crenarchaeal thermoacidophiles, the chromosomal spacers show a high level of matches (∼30%) with viral or plasmid genomes. Moreover, their distribution along the virus/plasmid genomes, as well as their DNA strand specificity, appear to be random. This is consistent with the hypothesis that chromosomal spacers are taken up directly and randomly from virus and plasmid DNA and that the spacer transcripts target the genomic DNA of the extrachromosomal elements and not their transcripts.

APA, Harvard, Vancouver, ISO, and other styles

6

Dinkla, Inez J. T., Mariekie Gericke, B. K. Geurkink, and Kevin B. Hallberg. "Acidianus Brierleyi is the Dominant Thermoacidophile in a Bioleaching Community Processing Chalcopyrite Containing Concentrates at 70°C." Advanced Materials Research 71-73 (May 2009): 67–70. http://dx.doi.org/10.4028/www.scientific.net/amr.71-73.67.

Full text

Abstract:

Bioleaching test work was performed in continuously operated multi-stage reactor systems at 70°C using a thermophilic culture treating an Aguablanca Ni-Cu concentrate from Spain and a blend of Cu concentrates from Bor, Serbia. The copper in both these concentrates occurs as chalcopyrite and therefore the use of thermophiles was applied, which resulted in copper recoveries of over 95%. Qualitative assessment of the microbial community in the bioreactors was performed by terminal restriction enzyme fragment length polymorphism (T-RFLP) and clone library analysis of the 16S rRNA genes amplified by PCR. T-RFLP analysis revealed that only archaea were present, and that the communities in both the Aguablanca and Bor systems consisted of two different microorganisms. A 16S rRNA gene clone library using DNA from the Aguablanca system was constructed and screened. Again, two archaea were detected in similar relative abundance in the population as found by T-RFLP analyses. The sequences of these two cloned genes revealed that the dominant archaeon (up to 98% of the total archaea detected) was Acidianus brierleyi, and the other was Metallosphaera sedula. Quantitative assessment of the microbial community was performed by Q-PCR and confirmed the dominance of archaea in the system with Acidianus being the dominant strain (98-99% of the total population) and a minor part of the population (1-2%) consisted of Metallosphaera. Additionally, very small amounts of Sulfolobus spp. were detected. This study, along with other recent studies on the diversity of thermoacidophiles involved in the solubilization of copper from chalcopyrite concentrates, revealed that a wider variety of thermoacidophiles are involved in bioprocessing of metal sulfides, and showed that A. brierleyi should be considered an important biomining acidophile.

APA, Harvard, Vancouver, ISO, and other styles

7

Flores, Gilberto E., Ryan C. Hunter, Yitai Liu, Anchelique Mets, Stefan Schouten, and Anna-Louise Reysenbach. "Hippea jasoniae sp. nov. and Hippea alviniae sp. nov., thermoacidophilic members of the class Deltaproteobacteria isolated from deep-sea hydrothermal vent deposits." International Journal of Systematic and Evolutionary Microbiology 62, Pt_6 (June 1, 2012): 1252–58. http://dx.doi.org/10.1099/ijs.0.033001-0.

Full text

Abstract:

Thirteen novel, obligately anaerobic, thermoacidophilic bacteria were isolated from deep-sea hydrothermal vent sites. Four of the strains, designated EP5-rT, KM1, Mar08-272rT and Mar08-368r, were selected for metabolic and physiological characterization. With the exception of strain EP5-rT, all strains were short rods that grew between 40 and 72 °C, with optimal growth at 60–65 °C. Strain EP5-rT was more ovoid in shape and grew between 45 and 75 °C, with optimum growth at 60 °C. The pH range for growth of all the isolates was between pH 3.5 and 5.5 (optimum pH 4.5 to 5.0). Strain Mar08-272rT could only grow up to pH 5.0. Elemental sulfur was required for heterotrophic growth on acetate, succinate, Casamino acids and yeast extract. Strains EP5-rT, Mar08-272rT and Mar08-368r could also use fumarate, while strains EP5-rT, KM1 and Mar08-272rT could also use propionate. All isolates were able to grow chemolithotrophically on H2, CO2, sulfur and vitamins. Phylogenetic analysis of 16S rRNA gene sequences placed all isolates within the family Desulfurellaceae of the class Deltaproteobacteria , with the closest cultured relative being Hippea maritima MH2 T (~95–98 % gene sequence similarity). Phylogenetic analysis also identified several isolates with at least one intervening sequence within the 16S rRNA gene. The genomic DNA G+C contents of strains EP5-rT, KM1, Mar08-272rT and Mar08-368r were 37.1, 42.0, 35.6 and 37.9 mol%, respectively. The new isolates differed most significantly from H. maritima MH2 T in their phylogenetic placement and in that they were obligate thermoacidophiles. Based on these phylogenetic and phenotypic properties, the following two novel species are proposed: Hippea jasoniae sp. nov. (type strain Mar08-272rT = DSM 24585T = OCM 985T) and Hippea alviniae sp. nov. (type strain EP5-rT = DSM 24586T = OCM 986T).

APA, Harvard, Vancouver, ISO, and other styles

8

Wheaton, Garrett H., Arpan Mukherjee, and Robert M. Kelly. "Transcriptomes of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Exposed to Metal “Shock” Reveal Generic and Specific Metal Responses." Applied and Environmental Microbiology 82, no. 15 (May 20, 2016): 4613–27. http://dx.doi.org/10.1128/aem.01176-16.

Full text

Abstract:

ABSTRACTThe extremely thermoacidophilic archaeonMetallosphaera sedulamobilizes metals by novel membrane-associated oxidase clusters and, consequently, requires metal resistance strategies. This issue was examined by “shocking”M. sedulawith representative metals (Co2+, Cu2+, Ni2+, UO22+, Zn2+) at inhibitory and subinhibitory levels. Collectively, one-quarter of the genome (554 open reading frames [ORFs]) responded to inhibitory levels, and two-thirds (354) of the ORFs were responsive to a single metal. Cu2+(259 ORFs, 106 Cu2+-specific ORFs) and Zn2+(262 ORFs, 131 Zn2+-specific ORFs) triggered the largest responses, followed by UO22+(187 ORFs, 91 UO22+-specific ORFs), Ni2+(93 ORFs, 25 Ni2+-specific ORFs), and Co2+(61 ORFs, 1 Co2+-specific ORF). While one-third of the metal-responsive ORFs are annotated as encoding hypothetical proteins, metal challenge also impacted ORFs responsible for identifiable processes related to the cell cycle, DNA repair, and oxidative stress. Surprisingly, there were only 30 ORFs that responded to at least four metals, and 10 of these responded to all five metals. This core transcriptome indicated induction of Fe-S cluster assembly (Msed_1656-Msed_1657), tungsten/molybdenum transport (Msed_1780-Msed_1781), and decreased central metabolism. Not surprisingly, a metal-translocating P-type ATPase (Msed_0490) associated with a copper resistance system (Cop) was upregulated in response to Cu2+(6-fold) but also in response to UO22+(4-fold) and Zn2+(9-fold). Cu2+challenge uniquely induced assimilatory sulfur metabolism for cysteine biosynthesis, suggesting a role for this amino acid in Cu2+resistance or issues in sulfur metabolism. The results indicate thatM. sedulaemploys a range of physiological and biochemical responses to metal challenge, many of which are specific to a single metal and involve proteins with yet unassigned or definitive functions.IMPORTANCEThe mechanisms by which extremely thermoacidophilic archaea resist and are negatively impacted by metals encountered in their natural environments are important to understand so that technologies such as bioleaching, which leverage microbially based conversion of insoluble metal sulfides to soluble species, can be improved. Transcriptomic analysis of the cellular response to metal challenge provided both global and specific insights into how these novel microorganisms negotiate metal toxicity in natural and technological settings. As genetics tools are further developed and implemented for extreme thermoacidophiles, information about metal toxicity and resistance can be leveraged to create metabolically engineered strains with improved bioleaching characteristics.

APA, Harvard, Vancouver, ISO, and other styles

9

Wheaton, Garrett, James Counts, Arpan Mukherjee, Jessica Kruh, and Robert Kelly. "The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles." Minerals 5, no. 3 (July 7, 2015): 397–451. http://dx.doi.org/10.3390/min5030397.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Rinker, K. D., C. J. Han, and R. M. Kelly. "Continuous culture as a tool for investigating the growth physiology of heterotrophic hyperthermophiles and extreme thermoacidophiles." Journal of Applied Microbiology 85, S1 (December 1998): 118S—127S. http://dx.doi.org/10.1111/j.1365-2672.1998.tb05290.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

KOCABIYIK, S., and B. ERDEM. "Intracellular alkaline proteases produced by thermoacidophiles: detection of protease heterogeneity by gelatin zymography and polymerase chain reaction (PCR)." Bioresource Technology 84, no. 1 (August 2002): 29–33. http://dx.doi.org/10.1016/s0960-8524(02)00019-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Alharbi, Faisal, Thomas Knura, Bettina Siebers, and Kesen Ma. "Thermostable and O2-Insensitive Pyruvate Decarboxylases from Thermoacidophilic Archaea Catalyzing the Production of Acetaldehyde." Biology 11, no. 8 (August 22, 2022): 1247. http://dx.doi.org/10.3390/biology11081247.

Full text

Abstract:

Pyruvate decarboxylase (PDC) is a key enzyme involved in ethanol fermentation, and it catalyzes the decarboxylation of pyruvate to acetaldehyde and CO2. Bifunctional PORs/PDCs that also have additional pyruvate:ferredoxin oxidoreductase (POR) activity are found in hyperthermophiles, and they are mostly oxygen-sensitive and CoA-dependent. Thermostable and oxygen-stable PDC activity is highly desirable for biotechnological applications. The enzymes from the thermoacidophiles Saccharolobus (formerly Sulfolobus) solfataricus (Ss, Topt = 80 °C) and Sulfolobus acidocaldarius (Sa, Topt = 80 °C) were purified and characterized, and their biophysical and biochemical properties were determined comparatively. Both enzymes were shown to be heterodimeric, and their two subunits were determined by SDS-PAGE to be 37 ± 3 kDa and 65 ± 2 kDa, respectively. The purified enzymes from S. solfataricus and S. acidocaldarius showed both PDC and POR activities which were CoA-dependent, and they were thermostable with half-life times of 2.9 ± 1 and 1.1 ± 1 h at 80 °C, respectively. There was no loss of activity in the presence of oxygen. Optimal pH values for their PDC and POR activity were determined to be 7.9 and 8.6, respectively. In conclusion, both thermostable SsPOR/PDC and SaPOR/PDC catalyze the CoA-dependent production of acetaldehyde from pyruvate in the presence of oxygen.

APA, Harvard, Vancouver, ISO, and other styles

13

Allan, R. N., L. Lebbe, J. Heyrman, P. De Vos, C. J. Buchanan, and N. A. Logan. "Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica." International Journal of Systematic and Evolutionary Microbiology 55, no. 3 (May 1, 2005): 1039–50. http://dx.doi.org/10.1099/ijs.0.63397-0.

Full text

Abstract:

Thirteen strains of endospore-forming bacteria were isolated from geothermal soils at Cryptogam Ridge, the north-west slope of Mt Melbourne, and at the vents and summit of Mt Rittmann in northern Victoria Land, Antarctica. 16S rRNA gene sequencing, SDS-PAGE and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of Mt Melbourne represent a novel species of Brevibacillus and that the six isolates from Cryptogam Ridge and the vents and summit of Mt Rittmann represent a novel species of Aneurinibacillus. Brevibacillus strains were not isolated from the sites at Mt Rittmann or Cryptogam Ridge and Aneurinibacillus strains were not isolated from the north-west slope of Mt Melbourne. Preliminary metabolic studies revealed that l-glutamic acid, although not essential for growth, was utilized by both species. The Brevibacillus species possessed an uptake system specific for l-glutamic acid, whereas the Aneurinibacillus species possessed a more general uptake system capable of transporting other related amino acids. Both species utilized a K+ antiport system and similar energy systems for the uptake of l-glutamic acid. The rate of uptake by the Brevibacillus species type strain was 20-fold greater than that shown by the Aneurinibacillus species type strain. The names Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov. are proposed for the novel taxa; the type strains are Logan B-1657T (=LMG 22481T=CIP 108307T) and Logan B-1599T (LMG 22483T=CIP 108308T), respectively.

APA, Harvard, Vancouver, ISO, and other styles

14

Bonanno, Alexander, and Parkson Lee-Gau Chong. "Certain, but Not All, Tetraether Lipids from the Thermoacidophilic Archaeon Sulfolobus acidocaldarius Can Form Black Lipid Membranes with Remarkable Stability and Exhibiting Mthk Channel Activity with Unusually High Ca2+ Sensitivity." International Journal of Molecular Sciences 22, no. 23 (November 30, 2021): 12941. http://dx.doi.org/10.3390/ijms222312941.

Full text

Abstract:

Bipolar tetraether lipids (BTL) have been long thought to play a critical role in allowing thermoacidophiles to thrive under extreme conditions. In the present study, we demonstrated that not all BTLs from the thermoacidophilic archaeon Sulfolobus acidocaldarius exhibit the same membrane behaviors. We found that free-standing planar membranes (i.e., black lipid membranes, BLM) made of the polar lipid fraction E (PLFE) isolated from S. acidocaldarius formed over a pinhole on a cellulose acetate partition in a dual-chamber Teflon device exhibited remarkable stability showing a virtually constant capacitance (~28 pF) for at least 11 days. PLFE contains exclusively tetraethers. The dominating hydrophobic core of PLFE lipids is glycerol dialky calditol tetraether (GDNT, ~90%), whereas glycerol dialkyl glycerol tetraether (GDGT) is a minor component (~10%). In sharp contrast, BLM made of BTL extracted from microvesicles (Sa-MVs) released from the same cells exhibited a capacitance between 36 and 39 pF lasting for only 8 h before membrane dielectric breakdown. Lipids in Sa-MVs are also exclusively tetraethers; however, the dominating lipid species in Sa-MVs is GDGT (>99%), not GDNT. The remarkable stability of BLMPLFE can be attributed to strong PLFE–PLFE and PLFE–substrate interactions. In addition, we compare voltage-dependent channel activity of calcium-gated potassium channels (MthK) in BLMPLFE to values recorded in BLMSa-MV. MthK is an ion channel isolated from a methanogenic that has been extensively characterized in diester lipid membranes and has been used as a model for calcium-gated potassium channels. We found that MthK can insert into BLMPLFE and exhibit channel activity, but not in BLMSa-MV. Additionally, the opening/closing of the MthK in BLMPLFE is detectable at calcium concentrations as low as 0.1 mM; conversely, in diester lipid membranes at such a low calcium concentration, no MthK channel activity is detectable. The differential effect of membrane stability and MthK channel activity between BLMPLFE and BLMSa-MV may be attributed to their lipid structural differences and thus their abilities to interact with the substrate and membrane protein. Since Sa-MVs that bud off from the plasma membrane are exclusively tetraether lipids but do not contain the main tetraether lipid component GDNT of the plasma membrane, domain segregation must occur in S. acidocaldarius. The implication of this study is that lipid domain formation is existent and functionally essential in all kinds of cells, but domain formation may be even more prevalent and pronounced in hyperthermophiles, as strong domain formation with distinct membrane behaviors is necessary to counteract randomization due to high growth temperatures while BTL in general make archaea cell membranes stable in high temperature and low pH environments whereas different BTL domains play different functional roles.

APA, Harvard, Vancouver, ISO, and other styles

15

Reysenbach, Anna-Louise, Emily St. John, Jennifer Meneghin, Gilberto E. Flores, Mircea Podar, Nina Dombrowski, Anja Spang, et al. "Complex subsurface hydrothermal fluid mixing at a submarine arc volcano supports distinct and highly diverse microbial communities." Proceedings of the National Academy of Sciences 117, no. 51 (December 4, 2020): 32627–38. http://dx.doi.org/10.1073/pnas.2019021117.

Full text

Abstract:

Hydrothermally active submarine volcanoes are mineral-rich biological oases contributing significantly to chemical fluxes in the deep sea, yet little is known about the microbial communities inhabiting these systems. Here we investigate the diversity of microbial life in hydrothermal deposits and their metagenomics-inferred physiology in light of the geological history and resulting hydrothermal fluid paths in the subsurface of Brothers submarine volcano north of New Zealand on the southern Kermadec arc. From metagenome-assembled genomes we identified over 90 putative bacterial and archaeal genomic families and nearly 300 previously unknown genera, many potentially endemic to this submarine volcanic environment. While magmatically influenced hydrothermal systems on the volcanic resurgent cones of Brothers volcano harbor communities of thermoacidophiles and diverse members of the superphylum “DPANN,” two distinct communities are associated with the caldera wall, likely shaped by two different types of hydrothermal circulation. The communities whose phylogenetic diversity primarily aligns with that of the cone sites and magmatically influenced hydrothermal systems elsewhere are characterized predominately by anaerobic metabolisms. These populations are probably maintained by fluids with greater magmatic inputs that have interacted with different (deeper) previously altered mineral assemblages. However, proximal (a few meters distant) communities with gene-inferred aerobic, microaerophilic, and anaerobic metabolisms are likely supported by shallower seawater-dominated circulation. Furthermore, mixing of fluids from these two distinct hydrothermal circulation systems may have an underlying imprint on the high microbial phylogenomic diversity. Collectively our results highlight the importance of considering geologic evolution and history of subsurface processes in studying microbial colonization and community dynamics in volcanic environments.

APA, Harvard, Vancouver, ISO, and other styles

16

Angelov, A., and W. Liebl. "Insights into extreme thermoacidophily based on genome analysis of Picrophilus torridus and other thermoacidophilic archaea." Journal of Biotechnology 126, no. 1 (October 2006): 3–10. http://dx.doi.org/10.1016/j.jbiotec.2006.02.017.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Reysenbach, Anna-Louise, Krista Longnecker, and Julie Kirshtein. "Novel Bacterial and Archaeal Lineages from an In Situ Growth Chamber Deployed at a Mid-Atlantic Ridge Hydrothermal Vent." Applied and Environmental Microbiology 66, no. 9 (September 1, 2000): 3798–806. http://dx.doi.org/10.1128/aem.66.9.3798-3806.2000.

Full text

Abstract:

ABSTRACT The phylogenetic diversity was determined for a microbial community obtained from an in situ growth chamber placed on a deep-sea hydrothermal vent on the Mid-Atlantic Ridge (23Ã¯Â¿Â½22′ N, 44Ã¯Â¿Â½57′ W). The chamber was deployed for 5 days, and the temperature within the chamber gradually decreased from 70 to 20Ã¯Â¿Â½C. Upon retrieval of the chamber, the DNA was extracted and the small-subunit rRNA genes (16S rDNA) were amplified by PCR using primers specific for theArchaea or Bacteria domain and cloned. Unique rDNA sequences were identified by restriction fragment length polymorphisms, and 38 different archaeal and bacterial phylotypes were identified from the 85 clones screened. The majority of the archaeal sequences were affiliated with the Thermococcales (71%) and Archaeoglobales (22%) orders. A sequence belonging to the Thermoplasmales confirms that thermoacidophiles may have escaped enrichment culturing attempts of deep-sea hydrothermal vent samples. Additional sequences that represented deeply rooted lineages in the low-temperature eurarchaeal (marine group II) and crenarchaeal clades were obtained. The majority of the bacterial sequences obtained were restricted to the Aquificales(18%), the ɛ subclass of the Proteobacteria(ɛ-Proteobacteria) (40%), and the genusDesulfurobacterium (25%). Most of the clones (28%) were confined to a monophyletic clade within the ɛ-Proteobacteria with no known close relatives. The prevalence of clones related to thermophilic microbes that use hydrogen as an electron donor and sulfur compounds (S0, SO4, thiosulfate) indicates the importance of hydrogen oxidation and sulfur metabolism at deep-sea hydrothermal vents. The presence of sequences that are related to sequences from hyperthermophiles, moderate thermophiles, and mesophiles suggests that the diversity obtained from this analysis may reflect the microbial succession that occurred in response to the shift in temperature and possible associated changes in the chemistry of the hydrothermal fluid.

APA, Harvard, Vancouver, ISO, and other styles

18

Han, Yejun, Aaron S. Hawkins, Michael W. W. Adams, and Robert M. Kelly. "Epimerase (Msed_0639) and Mutase (Msed_0638 and Msed_2055) Convert (S)-Methylmalonyl-Coenzyme A (CoA) to Succinyl-CoA in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Cycle." Applied and Environmental Microbiology 78, no. 17 (June 29, 2012): 6194–202. http://dx.doi.org/10.1128/aem.01312-12.

Full text

Abstract:

ABSTRACTCrenarchaeotal genomes encode the 3-hydroxypropionate/4-hydroxybutyrate (3-HP/4-HB) cycle for carbon dioxide fixation. Of the 13 enzymes putatively comprising the cycle, several of them, including methylmalonyl-coenzyme A (CoA) epimerase (MCE) and methylmalonyl-CoA mutase (MCM), which convert (S)-methylmalonyl-CoA to succinyl-CoA, have not been confirmed and characterized biochemically. In the genome ofMetallosphaera sedula(optimal temperature [Topt], 73°C), the gene encoding MCE (Msed_0639) is adjacent to that encoding the catalytic subunit of MCM-α (Msed_0638), while the gene for the coenzyme B12-binding subunit of MCM (MCM-β) is located remotely (Msed_2055). The expression of all three genes was significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO2fixation. Recombinant forms of MCE and MCM were produced inEscherichia coli; soluble, active MCM was produced only if MCM-α and MCM-β were coexpressed. MCE is a homodimer and MCM is a heterotetramer (α2β2) with specific activities of 218 and 2.2 μmol/min/mg, respectively, at 75°C. The heterotetrameric MCM differs from the homo- or heterodimeric orthologs in other organisms. MCE was activated by divalent cations (Ni2+, Co2+, and Mg2+), and the predicted metal binding/active sites were identified through sequence alignments with less-thermophilic MCEs. The conserved coenzyme B12-binding motif (DXHXXG-SXL-GG) was identified inM. sedulaMCM-β. The two enzymes together catalyzed the two-step conversion of (S)-methylmalonyl-CoA to succinyl-CoA, consistent with their proposed role in the 3-HP/4-HB cycle. Based on the highly conserved occurrence of single copies of MCE and MCM inSulfolobaceaegenomes, theM. sedulaenzymes are likely to be representatives of these enzymes in the 3-HP/4-HB cycle in crenarchaeal thermoacidophiles.

APA, Harvard, Vancouver, ISO, and other styles

19

McCarthy, Samuel, Tyler Johnson, Benjamin J. Pavlik, Sophie Payne, Wendy Schackwitz, Joel Martin, Anna Lipzen, Erica Keffeler, and Paul Blum. "Expanding the Limits of Thermoacidophily in the Archaeon Sulfolobus solfataricus by Adaptive Evolution." Applied and Environmental Microbiology 82, no. 3 (November 20, 2015): 857–67. http://dx.doi.org/10.1128/aem.03225-15.

Full text

Abstract:

ABSTRACTExtremely thermoacidophilicCrenarchaeotabelonging to the orderSulfolobalesflourish in hot acidic habitats that are strongly oxidizing. The pH extremes of these habitats, however, often exceed the acid tolerance of type species and strains. Here, adaptive laboratory evolution was used over a 3-year period to test whether such organisms harbor additional thermoacidophilic capacity. Three distinct cell lines derived from a single type species were subjected to high-temperature serial passage while culture acidity was gradually increased. A 178-fold increase in thermoacidophily was achieved after 29 increments of shifted culture pH resulting in growth at pH 0.8 and 80°C. These strains were named super-acid-resistantCrenarchaeota(SARC). Mathematical modeling using growth parameters predicted the limits of acid resistance, while genome resequencing and transcriptome resequencing were conducted for insight into mechanisms responsible for the evolved trait. Among the mutations that were detected, a set of eight nonsynonymous changes may explain the heritability of increased acid resistance despite an unexpected lack of transposition. Four multigene components of the SARC transcriptome implicated oxidative stress as a primary challenge accompanying growth at acid extremes. These components included accelerated membrane biogenesis, induction of themeroperon, and an increased capacity for the generation of energy and reductant.

APA, Harvard, Vancouver, ISO, and other styles

20

Fiore, Michele, and René Buchet. "Symmetry Breaking of Phospholipids." Symmetry 12, no. 9 (September 10, 2020): 1488. http://dx.doi.org/10.3390/sym12091488.

Full text

Abstract:

Either stereo reactants or stereo catalysis from achiral or chiral molecules are a prerequisite to obtain pure enantiomeric lipid derivatives. We reviewed a few plausibly organic syntheses of phospholipids under prebiotic conditions with special attention paid to the starting materials as pro-chiral dihydroxyacetone and dihydroxyacetone phosphate (DHAP), which are the key molecules to break symmetry in phospholipids. The advantages of homochiral membranes compared to those of heterochiral membranes were analysed in terms of specific recognition, optimal functions of enzymes, membrane fluidity and topological packing. All biological membranes contain enantiomerically pure lipids in modern bacteria, eukarya and archaea. The contemporary archaea, comprising of methanogens, halobacteria and thermoacidophiles, are living under extreme conditions reminiscent of primitive environment and may indicate the origin of one ancient evolution path of lipid biosynthesis. The analysis of the known lipid metabolism reveals that all modern cells including archaea synthetize enantiomerically pure lipid precursors from prochiral DHAP. Sn-glycerol-1-phosphate dehydrogenase (G1PDH), usually found in archaea, catalyses the formation of sn-glycerol-1-phosphate (G1P), while sn-glycerol-3-phosphate dehydrogenase (G3PDH) catalyses the formation of sn-glycerol-3-phosphate (G3P) in bacteria and eukarya. The selective enzymatic activity seems to be the main strategy that evolution retained to obtain enantiomerically pure lipids. The occurrence of two genes encoding for G1PDH and G3PDH served to build up an evolutionary tree being the basis of our hypothesis article focusing on the evolution of these two genes. Gene encoding for G3PDH in eukarya may originate from G3PDH gene found in rare archaea indicating that archaea appeared earlier in the evolutionary tree than eukarya. Archaea and bacteria evolved probably separately, due to their distinct respective genes coding for G1PDH and G3PDH. We propose that prochiral DHAP is an essential molecule since it provides a convergent link between G1DPH and G3PDH. The synthesis of enantiopure phospholipids from DHAP appeared probably firstly in the presence of chemical catalysts, before being catalysed by enzymes which were the products of later Darwinian selection. The enzymes were probably selected for their efficient catalytic activities during evolution from large libraries of vesicles containing amino acids, carbohydrates, nucleic acids, lipids, and meteorite components that induced symmetry imbalance.

APA, Harvard, Vancouver, ISO, and other styles

21

Mardanov, Andrey V., Vitali A. Svetlitchnyi, Alexey V. Beletsky, Maria I. Prokofeva, Elizaveta A. Bonch-Osmolovskaya, Nikolai V. Ravin, and Konstantin G. Skryabin. "The Genome Sequence of the Crenarchaeon Acidilobus saccharovorans Supports a New Order, Acidilobales, and Suggests an Important Ecological Role in Terrestrial Acidic Hot Springs." Applied and Environmental Microbiology 76, no. 16 (June 25, 2010): 5652–57. http://dx.doi.org/10.1128/aem.00599-10.

Full text

Abstract:

ABSTRACT Acidilobus saccharovorans is an anaerobic, organotrophic, thermoacidophilic crenarchaeon isolated from a terrestrial hot spring. We report the complete genome sequence of A. saccharovorans, which has permitted the prediction of genes for Embden-Meyerhof and Entner-Doudoroff pathways and genes associated with the oxidative tricarboxylic acid cycle. The electron transfer chain is branched with two sites of proton translocation and is linked to the reduction of elemental sulfur and thiosulfate. The genomic data suggest an important role of the order Acidilobales in thermoacidophilic ecosystems whereby its members can perform a complete oxidation of organic substrates, closing the anaerobic carbon cycle.

APA, Harvard, Vancouver, ISO, and other styles

22

Ree, Heesoo K., Kaiming Cao, David L. Thurlow, and Robert A. Zimmermann. "The structure and organization of the 16S ribosomal RNA gene from the archaebacterium Thermoplasma acidophilum." Canadian Journal of Microbiology 35, no. 1 (January 1, 1989): 124–33. http://dx.doi.org/10.1139/m89-019.

Full text

Abstract:

The complete nucleotide sequence of the 16S rRNA gene from Thermoplasma acidophilum, as well as its 5′ and 3′ flanking regions, were determined by the dideoxynucleotide chain termination method. The 16S rRNA gene encodes 1471 nucleotides. The primary and secondary structures of T. acidophilum 16S rRNA both exhibit typical archaebacterial features. The sequence appears to be more closely related to 16S rRNAs of the methanogen–halophile group than to those of the thermoacidophile group. Secondary-structure comparisons generally support this relationship, although there are several examples in which the single-stranded loops in particular helices of T. acidophilum 16S rRNA more strongly resemble their counterparts in the 16S rRNA of Sulfolobus solfataricus, a member of the thermoacidophile group. In contrast to the polycistronic rRNA operons found in most organisms, the three rRNA genes from T. acidophilum occur in only a single copy per genome and appear to be physically unlinked. Consistent with this, the 16S rRNA gene is flanked by putative promoter and terminator sequences that are comparable to the transcription control signals from other archaebacterial genes. The sequence TATATATA, which is very similar to the archaebacterial promoter consensus TTTAT/AATA, is located 18 bases before the probable site of transcription initiation, TGCACAT. There is a potential transcription termination site immediately downstream from the gene that consists of a relatively stable stem and loop structure followed by stretches of Tresidues.Key words: archaebacteria, thermoacidophile, rRNA sequence, rRNA secondary structure, promoter.

APA, Harvard, Vancouver, ISO, and other styles

23

Hess, Matthias. "Thermoacidophilic proteins for biofuel production." Trends in Microbiology 16, no. 9 (September 2008): 414–19. http://dx.doi.org/10.1016/j.tim.2008.06.001.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Semrau, Jeremy D., Alan A. DiSpirito, and J. Colin Murrell. "Life in the extreme: thermoacidophilic methanotrophy." Trends in Microbiology 16, no. 5 (May 2008): 190–93. http://dx.doi.org/10.1016/j.tim.2008.02.004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Bertoldo, C., C. Dock, and G. Antranikian. "Thermoacidophilic Microorganisms and their Novel Biocatalysts." Engineering in Life Sciences 4, no. 6 (December 2004): 521–32. http://dx.doi.org/10.1002/elsc.200402155.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Zentgraf, B., G. Rogge, and B. Nagel. "A thermoacidophilic bacterium similar toBacillus acidocaldarius." Acta Biotechnologica 12, no. 6 (1992): 481–87. http://dx.doi.org/10.1002/abio.370120608.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Hemmi, Hisashi, Satoshi Yamashita, Takefumi Shimoyama, Toru Nakayama, and Tokuzo Nishino. "Cloning, Expression, and Characterization ofcis-Polyprenyl Diphosphate Synthase from the Thermoacidophilic Archaeon Sulfolobus acidocaldarius." Journal of Bacteriology 183, no. 1 (January 1, 2001): 401–4. http://dx.doi.org/10.1128/jb.183.1.401-404.2001.

Full text

Abstract:

ABSTRACT cis-polyprenyl diphosphate synthases are involved in the biosynthesis of the glycosyl carrier lipid in most organisms. However, only little is known about this enzyme of archaea. In this report, we isolated the gene of cis-polyprenyl diphosphate synthase from a thermoacidophilic archaeon, Sulfolobus acidocaldarius, and characterized the recombinant enzyme.

APA, Harvard, Vancouver, ISO, and other styles

28

Morana, A., A. Esposito, L. Maurelli, G. Ruggiero, E. Ionata, M. Rossi, and F. Cara. "A Novel Thermoacidophilic Cellulase from Alicyclobacillus acidocaldarius." Protein & Peptide Letters 15, no. 9 (September 1, 2008): 1017–21. http://dx.doi.org/10.2174/092986608785849209.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Kufner, Kristina, and Georg Lipps. "Construction of a chimeric thermoacidophilic beta-endoglucanase." BMC Biochemistry 14, no. 1 (2013): 11. http://dx.doi.org/10.1186/1471-2091-14-11.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Gulik, A., V. Luzzati, M. DeRosa, and A. Gambacorta. "Tetraether lipid components from a thermoacidophilic archaebacterium." Journal of Molecular Biology 201, no. 2 (May 1988): 429–35. http://dx.doi.org/10.1016/0022-2836(88)90149-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

PRANGISHVILLI, David, Wolfram ZILLIG, Alfons GIERL, Lothar BIESERT, and Ingelore HOLZ. "DNA-Dependent RNA Polymerases of Thermoacidophilic Archaebacteria." European Journal of Biochemistry 122, no. 3 (March 3, 2005): 471–77. http://dx.doi.org/10.1111/j.1432-1033.1982.tb06461.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Görisch, H., T. Hartl, W. Grossebüter, and J. J. Stezowski. "Archaebacterial malate dehydrogenases. The enzymes from the thermoacidophilic organisms Sulfolobus acidocaldarius and Thermoplasma acidophilum show A-side stereospecificity for NAD+." Biochemical Journal 226, no. 3 (March 15, 1985): 885–88. http://dx.doi.org/10.1042/bj2260885.

Full text

Abstract:

The stereoselective transfer of hydrogen from NADH to oxaloacetate catalysed by malate dehydrogenases (EC 1.1.1.37) from the thermoacidophilic archaebacteria Sulfolobus acidocaldarius and Thermoplasma acidophilum was studied by the p.m.r. method described by Zhou & Wong [(1981) J. Biochem. Biophys. Methods 4, 329-338]. Both enzymes are A-side (pro-R) stereospecific for NADH.

APA, Harvard, Vancouver, ISO, and other styles

33

Lee, Sun-Young, Richard H. Dougherty, and Dong-Hyun Kang. "Inhibitory Effects of High Pressure and Heat on Alicyclobacillus acidoterrestris Spores in Apple Juice." Applied and Environmental Microbiology 68, no. 8 (August 2002): 4158–61. http://dx.doi.org/10.1128/aem.68.8.4158-4161.2002.

Full text

Abstract:

ABSTRACT The effectiveness of combined high pressure and heat treatment for reducing spore levels of Alicyclobacillus acidoterrestris, a thermoacidophilic spore-forming bacterium, in commercial pasteurized apple juice was investigated. Spores suspended in apple juice were successfully destroyed by combining high pressure with a mild or high temperature (45, 71, or 90°C).

APA, Harvard, Vancouver, ISO, and other styles

34

Song, Jin Long, Shuang Jiang Liu, and Cheng Ying Jiang. "Bioleaching of Chalcopyrite by Thermophilic Archaea." Advanced Materials Research 1130 (November 2015): 338–41. http://dx.doi.org/10.4028/www.scientific.net/amr.1130.338.

Full text

Abstract:

Bioleaching and biooxidation of sulfidicores and concentrates generate very high acidities and a great of heat, which rise the temperature in the reactors or heaps, and accumulate the sulfur on the surface of the ores. Extremely thermoacidophilic archaea, mainly from the genus ofAcidianus, Sulfolobus,Metallosphaeraandsulfurisphaera, have great potential to contribute to biomining processes for their inherent tolerance for low pH, high temperature, and high-soluble metal concentrations. Species of the genusMetallosphaeratypically grow by aerobic respiration on CO2with S0, tetrathionate (S4O62+), and Fe2+as electron donors, particularly suitble for metal extraction under high temperature by their iron- and sulfur-oxidation ability. Several species fromMetallosphaeraandAcidianusgenerawere investigated for their ability and conditions to dissolve various ores under a range of conditions. All of them showed good performance in copper extraction from chalcopyrite, with strainM.cuprinaAr-4 displaying higher activity than others. Surface analysis of chalcopyrite leached with the strain showed the leaching products accumulated on the ores. Our study will cover new understandings on the application of these thermoacidophilic archaea in biomining.

APA, Harvard, Vancouver, ISO, and other styles

35

Auernik, Kathryne S., and Robert M. Kelly. "Impact of Molecular Hydrogen on Chalcopyrite Bioleaching by the Extremely Thermoacidophilic Archaeon Metallosphaera sedula." Applied and Environmental Microbiology 76, no. 8 (February 26, 2010): 2668–72. http://dx.doi.org/10.1128/aem.02016-09.

Full text

Abstract:

ABSTRACT Hydrogen served as a competitive inorganic energy source, impacting the CuFeS 2 bioleaching efficiency of the extremely thermoacidophilic archaeon Metallosphaera sedula. Open reading frames encoding key terminal oxidase and electron transport chain components were triggered by CuFeS2. Evidence of heterotrophic metabolism was noted after extended periods of bioleaching, presumably related to cell lysis.

APA, Harvard, Vancouver, ISO, and other styles

36

Potter, Simon. "Evidence for a dual-specificity isocitrate dehydrogenase in the euryarchaeotan Thermoplasma acidophilum." Canadian Journal of Microbiology 39, no. 2 (February 1, 1993): 262–64. http://dx.doi.org/10.1139/m93-037.

Full text

Abstract:

The thermoacidophilic euryarchaeotan Thermoplasma acidophilum occupies a paradoxical place in phylogenetic trees. It has been found to possess both NAD- and NADP-dependent isocitrate dehydrogenase activities. Kinetic evidence presented suggests that both activities are functions of the same protein. The significance of this result, evolutionary and otherwise, is discussed.Key words: archaea, isocitrate dehydrogenase, molecular evolution.

APA, Harvard, Vancouver, ISO, and other styles

37

Rivero, Matías, Constanza Torres-Paris, Rodrigo Muñoz, Ricardo Cabrera, Claudio A. Navarro, and Carlos A. Jerez. "Inorganic Polyphosphate, Exopolyphosphatase, andPho84-Like Transporters May Be Involved in Copper Resistance inMetallosphaera sedulaDSM 5348T." Archaea 2018 (2018): 1–12. http://dx.doi.org/10.1155/2018/5251061.

Full text

Abstract:

Polyphosphates (PolyP) are linear polymers of orthophosphate residues that have been proposed to participate in metal resistance in bacteria and archaea. In addition of having a CopA/CopB copper efflux system, the thermoacidophilic archaeonMetallosphaera sedulacontains electron-dense PolyP-like granules and a putative exopolyphosphatase (PPXMsed,Msed_0891) and four presumedpho84-like phosphate transporters (Msed_0846,Msed_0866,Msed_1094, andMsed_1512) encoded in its genome. In the present report, the existence of a possible PolyP-based copper-resistance mechanism inM. sedulaDSM 5348Twas evaluated.M. sedulaDSM 5348Taccumulated high levels of phosphorous in the form of granules, and its growth was affected in the presence of 16 mM copper. PolyP levels were highly reduced after the archaeon was subjected to an 8 mM CuSO4shift. PPXMsedwas purified, and the enzyme was found to hydrolyze PolyPin vitro. Essential residues for catalysis of PPXMsedwere E111 and E113 as shown by a site-directed mutagenesis of the implied residues. Furthermore,M. sedula ppx,pho84-like, andcopTMAgenes were upregulated upon copper exposure, as determined by qRT-PCR analysis. The results obtained support the existence of a PolyP-dependent copper-resistance system that may be of great importance in the adaptation of this thermoacidophilic archaeon to its harsh environment.

APA, Harvard, Vancouver, ISO, and other styles

38

Giardina, P., M. G. de Biasi, M. de Rosa, A. Gambacorta, and V. Buonocore. "Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus." Biochemical Journal 239, no. 3 (November 1, 1986): 517–22. http://dx.doi.org/10.1042/bj2390517.

Full text

Abstract:

Glucose dehydrogenase has been purified to homogeneity from cell extracts of the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus. The enzyme utilizes both NAD+ and NADP+ as coenzyme and catalyses the oxidation of several monosaccharides to the corresponding glyconic acid. Substrate specificity and oxidation rate depend on the coenzyme present; when NAD+ is used, the enzyme binds and oxidizes specifically sugars presenting equatorial orientation of hydroxy groups at C-2, C-3 and C-4. The Mr of the native enzyme is 124,000 and decreases to about 60,000 in the presence of 6 M-guanidinium chloride and to about 30,000 in the presence of 5% (w/v) SDS. The enzyme shows maximal activity at pH 9, 77 degrees C and 20 mM-Mg2+, -Mn2+ or -Ca2+ and is fairly stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol or acetone.

APA, Harvard, Vancouver, ISO, and other styles

39

Fujii, T., Y. Hata, M. Ohzeki, H. Moriyama, T. Wakagi, N. Tanaka, and T. Oshima. "Refined crystal structure of ferredoxin from thermoacidophilic archaeon." Acta Crystallographica Section A Foundations of Crystallography 52, a1 (August 8, 1996): C233. http://dx.doi.org/10.1107/s010876739609006x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Krasil'nikova, E. N., T. I. Bogdanova, L. M. Zakharchuk, and I. A. Tsaplina. "Sulfur-Metabolizing Enzymes in Thermoacidophilic Bacteria Sulfobacillus sibiricus." Applied Biochemistry and Microbiology 40, no. 1 (January 2004): 53–56. http://dx.doi.org/10.1023/b:abim.0000010352.40671.af.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Umrania, Valentina V., and Jitendra S. Joshi. "Screening of Thermoacidophilic Autotrophic Bacteria for Covellite Solubilization." Applied Biochemistry and Biotechnology 102-103, no. 1-6 (2002): 359–66. http://dx.doi.org/10.1385/abab:102-103:1-6:359.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

CONSALVI, Valerio, Roberta CHIARALUCE, Laura POLITI, Agata GAMBACORTA, Mario ROSA, and Roberto SCANDURRA. "Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus." European Journal of Biochemistry 196, no. 2 (March 1991): 459–67. http://dx.doi.org/10.1111/j.1432-1033.1991.tb15837.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Iwasaki, Toshio, Toshiharu Suzuki, Takahide Kon, Takeo Imai, Akio Urushiyama, Daijiro Ohmori, and Tairo Oshima. "Novel Zinc-containing Ferredoxin Family in Thermoacidophilic Archaea." Journal of Biological Chemistry 272, no. 6 (February 7, 1997): 3453–58. http://dx.doi.org/10.1074/jbc.272.6.3453.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Wakagi, Takayoshi, and Tairo Oshima. "Energy metabolism of a thermoacidophilic archaebacterium,Sulfolobus acidocaldarius." Origins of Life and Evolution of the Biosphere 17, no. 3-4 (September 1987): 391–99. http://dx.doi.org/10.1007/bf02386477.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Anemüller, S., M. Lübben, and G. Schäfer. "The respiratory system ofSulfolobus acidocaldarius, a thermoacidophilic archaebacterium." FEBS Letters 193, no. 1 (November 25, 1985): 83–87. http://dx.doi.org/10.1016/0014-5793(85)80084-3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

SMITH, LEON D., STEPHEN J. BUNGARD, MICHAEL J. DANSON, and DAVID W. HOUGH. "Dihydrolipoamide dehydrogenase from the thermoacidophilic archaebacterium Thermoplasma acidophilum." Biochemical Society Transactions 15, no. 6 (December 1, 1987): 1097. http://dx.doi.org/10.1042/bst0151097.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Fujii, Tomomi, Yasuo Hata, Takayoshi Wakagi, Nobuo Tanaka, and Tairo Oshima. "Novel zinc-binding centre in thermoacidophilic archaeal ferredoxins." Nature Structural & Molecular Biology 3, no. 10 (October 1996): 834–37. http://dx.doi.org/10.1038/nsb1096-834.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Takagi, Masakazu, Hideyuki Tamaki, Yukiko Miyamoto, Roberta Leonardi, Satoshi Hanada, Suzanne Jackowski, and Shigeru Chohnan. "Pantothenate Kinase from the Thermoacidophilic Archaeon Picrophilus torridus." Journal of Bacteriology 192, no. 1 (October 23, 2009): 233–41. http://dx.doi.org/10.1128/jb.01021-09.

Full text

Abstract:

ABSTRACT Pantothenate kinase (CoaA) catalyzes the first step of the coenzyme A (CoA) biosynthetic pathway and controls the intracellular concentrations of CoA through feedback inhibition in bacteria. An alternative enzyme found in archaea, pantoate kinase, is missing in the order Thermoplasmatales. The PTO0232 gene from Picrophilus torridus, a thermoacidophilic euryarchaeon, is shown to be a distant homologue of the prokaryotic type I CoaA. The cloned gene clearly complements the poor growth of the temperature-sensitive Escherichia coli CoaA mutant strain ts9, and the recombinant protein expressed in E. coli cells transfers phosphate to pantothenate at pH 5 and 55°C. In contrast to E. coli CoaA, the P. torridus enzyme is refractory to feedback regulation by CoA, indicating that in P. torridus cells the CoA levels are not regulated by the CoaA step. These data suggest the existence of two subtypes within the class of prokaryotic type I CoaAs.

APA, Harvard, Vancouver, ISO, and other styles

49

Hamana, Koei, Shigeru Matsuzaki, Masaru Nitsu, Keijiro Samejima, and Hideyuki Nagashima. "Polyamines of unicellular thermoacidophilic red alga Cyanidium caldarium." Phytochemistry 29, no. 2 (January 1990): 377–80. http://dx.doi.org/10.1016/0031-9422(90)85082-q.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Lipps, Georg. "Plasmids and viruses of the thermoacidophilic crenarchaeote Sulfolobus." Extremophiles 10, no. 1 (January 6, 2006): 17–28. http://dx.doi.org/10.1007/s00792-005-0492-x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!