Dissertations / Theses on the topic 'Thermoacidophiles'
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ELIE, CHRISTIANE. "Adn polymerases d'archaebacteries : sensibilite des archaebacteries halophiles a l'aphidicoline : purification et caracterisation de deux adn polymerases chez deux archaebacteries thermoacidophiles." Paris 6, 1989. http://www.theses.fr/1989PA066172.
Full textWilliams, Timothy David. "Aspects of lithoautotrophy in iron-oxidizing thermoacidophilic archaea." Thesis, University of Warwick, 1995. http://wrap.warwick.ac.uk/78814/.
Full textGiroux, Xavier. "Etude du cycle viral de SSV1, un virus d'archaea thermoacidophile." Paris 11, 2010. http://www.theses.fr/2010PA112214.
Full textLn 1978, Carl Woese identified a third domain of life, the Archaea. These organisms were first identified in extreme environmental conditions, such as high temperature and high salt concentration. There are about fifty archaeal viruses currently identified, and they have a large variety of morphotypes. Among these viruses, the Fuselloviruses are the best characterized. They infect members of the Sulfolobus genus, isolated from sulfurous hot springs. There are currently nine Fuselloviruses genomes sequenced, with thirteen CDS common to all these viruses. Ln silico analysis predicted that one of these thirteen CDS, B251, could encode a bacterial-like replication initiator. We found that B251 have the characteristics of a bacterial-like replication initiator. We also identified a protein, which could be involved in replication initiation. B 129 is a zinc finger protein wich binds both to the origin of replication and close to the attP site, reminiscent of IHF in E. Coli. We performed in silico analyses of the nine Fuselloviruses genomes to identify their replication origin. Using GC skew analyses, we found that the Fuselloviruses could be divided in two subgroups, each of which replicate their DNA in a different way. All these results help to understand how the Fuselloviruses replicate their DNA and will provide a better understanding of their life cycle
Han, Chae Joon. "Physiological studies of extremely thermoacidophilic microorganisms undernormal and stressed conditions." Raleigh, NC : North Carolina State University, 1998. http://www.lib.ncsu.edu/etd/public/etd-1052132339841121/etd-title.html.
Full textBudgen, Nigel. "The catabolism of glucose by the thermoacidophilic archaebacterium Thermoplasma acidophilum." Thesis, University of Bath, 1988. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383178.
Full textSeipel, Kurtz. "Continuous growth and heat shock of thermoacidophilic Sulfolobus in a triple-stage chemostat for overexpression and isolation of chaperonin." Thesis, University of Iowa, 2012. https://ir.uiowa.edu/etd/2981.
Full textSmith, Leon David. "Studies of dehydrogenases from thermoacidophilic archaebacteria with a view to cofactor regeneration." Thesis, University of Bath, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328734.
Full textHelmecke, Julia Verfasser], Dietmar [Akademischer Betreuer] [Schomburg, and Dieter [Akademischer Betreuer] Jahn. "Vom Genom zum systemweiten Verständnis des Stoffwechsels thermoacidophiler Sulfolobales / Julia Helmecke ; Dietmar Schomburg, Dieter Jahn." Braunschweig : Technische Universität Braunschweig, 2019. http://d-nb.info/1198398833/34.
Full textAngelov, Angel Stoyanov. "Genome sequence analysis and characterization of recombinant enzymes from the thermoacidophilic archaeon Picrophilus torridus." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=974034916.
Full textAygar, Sema. "The Role Of Small Heat Shock Proteins Of The Thermoacidophilic Archaeon Thermoplasma Volcanium In The Stress Response." Master's thesis, METU, 2011. http://etd.lib.metu.edu.tr/upload/12613237/index.pdf.
Full textC for two hours. But, the second sHsp of the Tp. volcanium, TVN0984/sHsp was not effective in improvement of the thermal resistance of the mesophilic bacterium (i. e., E.coli). The expression of the TVN0775/sHsp and TVN0984/sHsp genes increased about 3 fold after heat-shock at 65°
C, as revealed by Real-Time PCR analysis. Although expression of the both genes was induced at 70°
C, TVN0984/sHsp gene expression was increased higher (about 5 fold) than that of the TVN0775/sHsp gene expression (about 1.5 fold). Tp. volcanium cells were exposed to high pH (pH: 3.5, pH: 4.0, pH: 4.5, pH: 5.0), and the change in the sHsp genes&rsquo
expression profile were analyzed. The results showed that TVN0775/sHsp gene expression was more sensitive to increased pH than TVN0984/sHsp gene expression. The TVN0775/sHsp gene transcription induced at most 2.5 fold at pH 4.0 and the gene expression either reduced or did not change at higher pH values (i.e., pH 4.5 and 5.0). On the other hand, TVN0984/sHsp gene expression did not change at pH 4.0 but significantly reduced at higher pH values. The effect of oxidative stress on the expression of TVN0775 and TVN0984 genes was investigated by treatment of Tp. volcanium cells with 0.01 mM, 0.02 mM, 0,03 mM and 0.05 mM H2O2. For both sHsp genes, transcription was induced at lower concentrations of H2O2 (0.01 mM and 0.02 mM). At higher concentrations of H2O2 expression of both genes&rsquo
transcription either did not changed or down regulated. Lastly, in this study we have purified the recombinant TVN0775/sHsp, as an Nterminal 6x his-tag fusion to homogeneity on Ni-NTA affinity column. Purified protein samples were used in the chaperone activity assays using bovine glutamate dehydrogenase enzyme (boGDH) as substrate. We have found that the recovery of glutamate dehydrogenase activity at 45°
C, 50°
C and 53°
C in the presence of the Tp. volcanium sHsps was higher than that of spontaneous refolding. Also, TVN0775/sHsp increased the recovery of the boGDH enzyme that was denatured at 2.5 M GdnHCl concentrations for 30 min.
Verheyen, Julia [Verfasser], and Bettina [Akademischer Betreuer] Siebers. "Sulfolobus acidocaldarius & sulfolobus solfataricus : exploitation of thermoacidophilic archaea for biotechnological applications / Julia Verheyen. Betreuer: Bettina Siebers." Duisburg, 2015. http://d-nb.info/1074825136/34.
Full textJachlewski, Silke [Verfasser], Bettina [Akademischer Betreuer] Siebers, and Hans-Curt [Akademischer Betreuer] Flemming. "Biofilm formation and EPS analysis of the thermoacidophilic Archaeon Sulfolobus acidocaldarius / Silke Jachlewski. Gutachter: Hans-Curt Flemming. Betreuer: Bettina Siebers." Duisburg, 2013. http://d-nb.info/1049578309/34.
Full textWolf, Jacqueline Verfasser], and Dietmar [Akademischer Betreuer] [Schomburg. "Metabolic adaptation of the thermoacidophilic crenarchaeon Sulfolobus solfataricus P2 in response to changing nutrient conditions / Jacqueline Wolf ; Betreuer: Dietmar Schomburg." Braunschweig : Technische Universität Braunschweig, 2017. http://d-nb.info/1175817554/34.
Full textBenninghoff, Jens Carlo [Verfasser], and Bettina [Akademischer Betreuer] Siebers. "The effect of organic solvents on biofilm formation of the thermoacidophilic Archaeon Sulfolobus acidocaldarius / Jens Carlo Benninghoff ; Betreuer: Bettina Siebers." Duisburg, 2020. http://d-nb.info/1220226386/34.
Full textMombelli, Enrico. "Stabilité conformationnelle et mécanisme de dénaturation de protéines thermostables : étude de la carboxypeptidase et de la ribonucléase Sso7D de l'archéobactérie thermoacidophile "Sulfolobus solfataricus"." Montpellier 2, 1999. http://www.theses.fr/1999MON20165.
Full textHaferkamp, Patrick [Verfasser], Bettina [Akademischer Betreuer] Siebers, and Daniel [Akademischer Betreuer] Hoffmann. "Biochemical studies of enzymes involved in glycolysis of the thermoacidophilic crenarchaeon Sulfolobus solfataricus / Patrick Haferkamp. Gutachter: Daniel Hoffmann. Betreuer: Bettina Siebers." Duisburg, 2011. http://d-nb.info/1017931879/34.
Full textSouza, Aryádina Mara Ribeiro de. "Atividade das bacteriocinas bovicina HC5 e nisina sobre o crescimento e a resistência térmica de Alicyclobacillus acidoterrestris em sucos de frutas." Universidade Federal de Viçosa, 2008. http://locus.ufv.br/handle/123456789/5297.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
Aguardando liberação do SIGILO
Aguardando liberação do SIGILO
CHO, YEN-LIN, and 卓宴琳. "Accumulation Mechanism of Lead in Thermoacidophilic Red Algae." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/32610290756479866880.
Full text東海大學
環境科學與工程學系
104
The thermoacidophilic red algae consist of three genus and seven species, which has been often found in sulfate-enriched hot spring. Such red algae were named as Cyanidiales that is able to tolerate high temperature and acidic environments. Due to such attributes, the Cyanidiales have been considered as a superior material to remove heavy metals such as lead from industrial wastewater and mining areas. In this study, we aimed to determine the retention capacity and develop the retention mechanisms of lead on Cyanidiales in relation to the lead speciation. The sorption isotherm of lead at pH 5.0 on six species of red algae including Galdieria maximum, Galdieria partita, Galdieria phlegrea, Galdieria sulphuraria, Cyanidium caldarium and Cyanidioschyzon merolae was first conducted. Lead retention mechanisms were determined using the Fourier Transform Infrared Spectroscopy (FTIR) and the linear combination fitting (LCF) of lead LIII-edge X-ray absorption spectroscopy (XAS). The sorption isotherm showed there was more than one step of sorption behaviors for Pb to retain on Cyanidiales. In the first step, the sorption capacity of lead on red algae was in the sequence of Cm (67.57 mg g-1) > Gs (34.84 mg g-1) > Cc (29.07 mg g-1) > Gph (24.33 mg g-1) > Gp (22.42 mg g-1) > Gm (12.85 mg g-1). After the first step, the greatest sorption capacity for each read algae was found in the sequence of Cc (299.36 mg g-1) > Cm (214.04 mg g-1) > Gp (128.64 mg g-1) > Gph (115.53 mg g-1) > Gs (108.77 mg g-1) > Gm (38.20 mg g-1). The FTIR analyses revealed that Amide I (C=O) and Amide II (C-N) functional groups were mainly responsible for the sorption of lead. Linear combination fitting of the XAS data showed that lead is mainly reacted with organic fractions in the system. The organic species of lead was determined as lead bound to the cell wall of plant and humate. The XAS data also suggested that lead might be precipitated on the surfaces of red algae. Conclusionly, the retention mechanisms of lead on Cyanidioales might be (1) lead bound with proteins and surface functional groups of the algae and (2) lead precipitated on the surfaces of the algae.
Ho, Wen-li, and 賀文麗. "Expression of Glucose Isomerase Gene from Thermoacidophile in Escherichia coli." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/57482038731574166390.
Full text東吳大學
微生物學系
93
High fructose corn syrup( HFCS ) is mixture of 55% fructose and 45% glucose. It is sweeter than sucrose by 1.3 fold and glucose by 1.7 fold. The production cost is lower than common sugars, and the raw material is easy to obtain. So it becomes the most widely used sweetener. The HFCS production from starch includes the isomerization of glucose to fructose, by glucose isomerase/ xylose isomerase. Using thermostable and acid tolerant glucose isomerase in HFCS production and improving the yield of glucose isomerase can lower the cost. We studied the expression of glucose isomerase gene, xylA, from the thermoacidophile, Alicyclobacillus acidocaldarius 49-1 isolated from the hot spring of Yangming Mountain. We cloned the xylA gene into expression vector pET-25b. The coding sequence of xylA was fused with a signal sequence, pelB and expressed under control of T7 promoter. The result appeared that the fusion protein formed the inclusion body. By decreasing the growth, it did not express active protein, but when growing in M9 minimum medium, active GI protein was produced apparently.
Jan, Ren-Long, and 詹仁隆. "A novel species of thermoacidophilic archaeon: Sulfolobus yangmingensis sp.nov." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/97450019528408808503.
Full textChiang, Yi-ting, and 江沂庭. "Characterization of recombinant ketol-acid reductoisomerase from thermoacidophilic archaeon Sulfolobus acidocaldarius DSM639." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/78174130242826535943.
Full text國立中央大學
生命科學研究所
100
Sulfolobus acidocaldarius DSM639 is a thermoacidophilic crenarchaeon which can grow in hot springs at 55-85 ˚C and acidic environments (pH 2-3). As we facing the extreme living environmental challenge now and in near future, the extreme adaptation genes, extremozymes and strategies from archaea may help us to adapt in hostile environments. In previous study, ketol-acid reductoisomerase (KARI, EC 1.1.1.86) was identified as one of numerous extra-cellular proteins of S. acidocaldarius DSM639 by LC/MS/MS. This enzyme not only participates in valine, leucine and isoleucine biosynthesis pathway, but also can be expressed in recombinant organism for bio-butanol production. In this study, KARI with GTG as start codon was overexpressed by recombinated E. coli system, and the biochemical properties of KARI were also explored. The results of N-terminal sequencing and DNA sequencing verified that KARI was expressed by using GTG as start codon in E. coli. It suggested that GTG can be used as start codon for protein expression in both archaeobacteria and E. coli. Furthermore, the KARI enzyme assay showed that optimal temperature and pH value of recombinant KARI was 65 ˚C and pH 7.3 in 100 mM HEPES-potassium containing 10 mM MgCl2 condition. The specific activity of purified enzyme was 3.5 μmole/min/mg, and the Km was 14 μM responding to co-substrate: NADPH.
郭曉卉. "Cloning of the Glucose Isomerase Gene from Thermoacidophile : Expression in Escherichia coli and Streptomyces." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/32840070374045555343.
Full text東吳大學
微生物學系
91
This research was dealt with the thermoacidophilic Bacillus acidocaldarius 49-1 isolated from the hot spring of Yangmin mountains in order to find its glucose isomerase ( GI )gene - xylA. We used a set of degenerate primers : XI-1 and XI-2 from literature. The primers consist conserved amino acid sequences of glucose isomerase( GI ) from two groups of microorganism. After performing polymerase chain reaction( PCR ), we obtained a specific 177 bp DNA fragment from strain 49-1. Using this fragment as radioactive probe to screen chromosomal DNA, 2.0 kb BamHI DNA fragment and 4.4 kb EcoRI DNA fragment were found. After cloning, pSJ16 and pSJ5 plasmids were obtained. By DNA sequencing and analysis, it is shown that pSJ5 includes complete xylA gene which encodes glucose isomerase. The xylA gene was subcloned into pBluescript II KS(+) vector to generate pSJ101 and pSJ102 plasmids. The xylA gene in pSJ102 was then transferred into pTrc99A, an expression vector which contains a strong trc promoter, for the construction of pSH201. Plasmid pSH201 expressed the GI activity in Escherichia coli strain HB101. The results were shown as following : (1) The specific activity of the GI in pSH201/E. coli HB101 is 100-fold of E. coli HB101 and pTrc99A / E. coli HB101, and threefold of strain 49-1. Moreover, the addition of IPTG does not increase GI activity of pSH201/E. coli HB101 obviously ; (2) The specific activity of pSH201/E. coli HB101 was increased 8-fold of strain 49-1 by heating at 70℃, indicating that the heat treatment can purify GI ; (3) From SDS-PAGE analysis, the molecular weight of GI is estimated about 49 kDa. Furthermore, plasmids pIS35 and pIS44 were obtained by the ligation of pSH201 with the Streptomyces plasmid pIJ702. The GI activity was proved when pIS35 and pIS44 were transformed them into E. coli HB101, but no activity was examined when they were in Streptomyces lividans TK64.
Ko, Shun-Wen, and 柯舜文. "Isolation of a Glucose Isomerase-Producing Thermoacidophile and the Purification and Characterization of its Enzyme." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/29476871109863270992.
Full text東吳大學
微生物學系
85
Abstract The purpose of the present study is to develop a glucose isomerase with a higher optimum temperature and thermostability and a lower optimum pH in order to solve problems in processing and thus lower costs and to increase the quality of high fructose corn syrup. The major results of the present study are as follows： 《1》The method used HEPES buffer containing magnesium and cobalt ions as thereacting medium and used a reacting agent containing glucose oxidase was selected as the analyzing method for the screening of glucose isomerase- producing strains. 《2》A solid medium using Gelrite in place of agar as the gelling agent was established for the isolation of thermoacidophiles (pH3, >60℃).The Allen''s basal salts medium supplemented with xylose (2g/l) and yest extract (0.1g/l)was used as the medium for the screening of glucose isomerase- producingstrains. 《3》Microorganisms in hot spring water as well as in surrounding soil throughout the area of Mt. Ta-Tun were screened. It was found that the glucose isomerase produced by a thermoacidophile No. 49-1 (its optimum growth temperature is around 70 ℃ and its optimum growth pH are between 2.5-4.5) is characterized by a higher optimum temperature (75-80 ℃) and a lower optimum pH (6.5). After studying some identification characters, this isolate was found to be most similar to Bacillus acidocaldarius. 《4》 This bacterial strain was grown in an air-lift fermemter. A large number of bacterial cells were collected and broken by sonication. A crude enzyme solution was then obtained. After the crude enzymes were treated by means of ion exchanger chromatography, gel filtration and t-ButylHIC cartridge, the specific activity of the glucose isomerase could be enhanced 5.6-fold. Recovery was 17.7%. Molecular weight of the purified enzyme was determined as 195,000 by gel filtration on Sephadex G-150 column.SDS-PAGE analysis showed a single band for the enzyme with an molecular weight of 45,000 indicating that the glucose isomerase was composed of homo-tetrameric subunits. T he activity of the purified enzyme had optimum temperatures between 75 ℃an d 80 ℃, and an optimum pH lower than or equal to 6.0. After being incubated in a buffer at pH 6-8 at 80 ℃ for half an hour, the enzyme still retained about 50% of its original activity. The thermostability test was performed at pH 6 and it was found that the enzyme still retained 70％ of its activity at 70℃ for an hour and 60％ of its activity at 80℃ for ten minitunes. Both manganese ion and cobalt ion have relevant enhancing effect on the enzyme''s activity.
Auernik, Kathryne Sherlock. "Functional genomics analysis of metal mobilization by the extremely thermoacidophilic archaeon Metallosphaera sedula." 2009. http://www.lib.ncsu.edu/theses/available/etd-03202009-213938/unrestricted/etd.pdf.
Full textAngelov, Angel. "Genome Sequence Analysis and Characterization of Recombinant Enzymes from the Thermoacidophilic Archaeon Picrophilus torridus." Doctoral thesis, 2004. http://hdl.handle.net/11858/00-1735-0000-0006-ACD9-E.
Full textTasi, G. K., and 蔡正國. "Partial characterization of a novel extracellular thermostable esterase from newly isolated thermoacidophilic Bacillus acidocaldarius strain A1." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/64040164499585018926.
Full text國立臺灣師範大學
生物研究所
88
ABSTRAT Partial characterization of a novel extracellular thermostable esterase from newly isolated thermoacidophilic Bacillus acidocaldarius strain A1 Several moderate thermophilic bacterial strains were isolated from hot acid spring about pH 3 and 55 degree C by enrichment methods. One of those was identified as Bacillus acidocaldarius strain A1. This strain grew under a narrow pH (pH 3~4) and moderate high temperature (45~65 degree C) condition and was optimal at pH 4 and 55 degree C. A thermostable esterase activity was detected in the culture medium supernatant when assayed by using the p-nitrophenyl ester. Adding olive oil to culture medium promoted bacterial growth, but had no effects on the esterase activity. The pH and temperature optima for the esterase activity were pH 7 and 75 degree C, respectively. This crude esterase activity was found capable of hydrolyzing long-chain fatty acid esters (pNPC12 to pNPC16) and exhibited thermal stability. It showed a half-life of 30 min at 75 degree C, and after 3 h incubation at 95 degree C, 18﹪of activity still remained. The effects of several kinds of salts, detergent, and chelating agent, EDTA were also examined. No ions used could promote the esterase activity. However, some detergents and ions had inhibitory effects on the enzyme, and EDTA had no inhibitory effects on it.
Chang, Tao-Tzu, and 張道慈. "A novel Archaeal Virus and a novel thermoacidophilic species isolated from Geothermal Valley of Bei Tou." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/26207942454530547298.
Full text國立陽明大學
微生物及免疫學研究所
96
We have identified a novel Archaea virus, named Acidianus dimorpho virus (ADV) from Geothermal Valley of Bei Tou, Taipei, Taiwan. Its host cell strain BT is a new isolate from the same locale. Based on its 16S rDNA sequence and DNA – DNA hybridization data, strain BT can be classified as a new species in the genus Acidianus. Its type strain is strain BTT. Strain BTT can used Fe3+ as electron acceptor under anaerobic condition. It can grow using S0 as an electron acceptor in anaerobic environment. The optimum growth temperature and pH of strain BTT was 75 oC and 3.0. When static cultured to the stationary phase, a biofilm-like structure appeared on water-gas interface which we called “white float”, We named the strain BTT a new species, Acidianus albonavi. ADV is a spindle-shaped dsDNA virus with a tail. When purified from host cells, its tail length ranges from 88 to 320 nm. Without UV or mitomycin induction, ADV started to lyse host cells 3 days after infection and released 80 viral particles was from each cell. In high ADV concentration, ADV could cause cells to expand to a “balloon cell” with diameters from 2 to 6 μm. Viewed under TEM, there were ADV particles in the balloon cell 3 days after infection. When we placed ADV in spring water of Geothermal Valley for 3 days, the virus transformed into a filament. Its length ranges from 466.7 to1213.3 nm. ADV is similar to STSV1 (Sulfolobus tengchongensis Spindle-Shaped Virus) in terms of both shape and amino acids sequence.
Angelov, Angel Stoyanov [Verfasser]. "Genome sequence analysis and characterization of recombinant enzymes from the thermoacidophilic archaeon Picrophilus torridus / vorgelegt von Angel Stoyanov Angelov." 2004. http://d-nb.info/974034916/34.
Full text