Relevant bibliographies by topics / Thermoacidophiles

Academic literature on the topic 'Thermoacidophiles'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Thermoacidophiles"

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Vetter, Anna M., Julia Helmecke, Dietmar Schomburg, and Meina Neumann-Schaal. "The Impact of Pyroglutamate:Sulfolobus acidocaldariusHas a Growth Advantage overSaccharolobus solfataricusin Glutamate-Containing Media." Archaea 2019 (April 24, 2019): 1–9. http://dx.doi.org/10.1155/2019/3208051.

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Microorganisms are well adapted to their habitat but are partially sensitive to toxic metabolites or abiotic compounds secreted by other organisms or chemically formed under the respective environmental conditions. Thermoacidophiles are challenged by pyroglutamate, a lactam that is spontaneously formed by cyclization of glutamate under aerobic thermoacidophilic conditions. It is known that growth of the thermoacidophilic crenarchaeonSaccharolobus solfataricus(formerlySulfolobus solfataricus) is completely inhibited by pyroglutamate. In the present study, we investigated the effect of pyroglutamate on the growth ofS. solfataricusand the closely related crenarchaeonSulfolobus acidocaldarius.In contrast toS. solfataricus,S. acidocaldariuswas successfully cultivated with pyroglutamate as a sole carbon source. Bioinformatical analyses showed that both members of theSulfolobaceaehave at least one candidate for a 5-oxoprolinase, which catalyses the ATP-dependent conversion of pyroglutamate to glutamate. InS. solfataricus, we observed the intracellular accumulation of pyroglutamate and crude cell extract assays showed a less effective degradation of pyroglutamate. Apparently,S. acidocaldariusseems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared toS. solfataricus. Concludingly,S. acidocaldariusexhibits a more efficient utilization of pyroglutamate and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures.
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Colman, Daniel R., Saroj Poudel, Trinity L. Hamilton, Jeff R. Havig, Matthew J. Selensky, Everett L. Shock, and Eric S. Boyd. "Geobiological feedbacks and the evolution of thermoacidophiles." ISME Journal 12, no. 1 (October 13, 2017): 225–36. http://dx.doi.org/10.1038/ismej.2017.162.

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Müller, Hans-Dieter, and Helmut Görisch. "Archaebacterial Citrate Synthases: The Enzymes from the Thermoacidophiles Sulfolobus acidocaldarius and Thermoplasma acidophilum Show pro-S Stereospecificity." Zeitschrift für Naturforschung C 44, no. 11-12 (December 1, 1989): 927–30. http://dx.doi.org/10.1515/znc-1989-11-1209.

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Abstract Citrate synthase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius was purified 365-fold to electrophoretic homogeneity. At 40 °C and pH 8.1 the homogeneous enzyme shows a specific activity of 73 units per mg, which corresponds to a turnover number of 44 sec-1. Citrate synthase from S. acidocaldarius shows pro-S stereospecificity, as is found with a partially purified preparation of the enzyme from Thermoplasma acidophilum, another thermoacidophilic archaebacterium.
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Chaban, Bonnie, Sandy Y. M. Ng, and Ken F. Jarrell. "Archaeal habitats — from the extreme to the ordinary." Canadian Journal of Microbiology 52, no. 2 (February 1, 2006): 73–116. http://dx.doi.org/10.1139/w05-147.

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The domain Archaea represents a third line of evolutionary descent, separate from Bacteria and Eucarya. Initial studies seemed to limit archaea to various extreme environments. These included habitats at the extreme limits that allow life on earth, in terms of temperature, pH, salinity, and anaerobiosis, which were the homes to hyper thermo philes, extreme (thermo)acidophiles, extreme halophiles, and methanogens. Typical environments from which pure cultures of archaeal species have been isolated include hot springs, hydrothermal vents, solfataras, salt lakes, soda lakes, sewage digesters, and the rumen. Within the past two decades, the use of molecular techniques, including PCR-based amplification of 16S rRNA genes, has allowed a culture-independent assessment of microbial diversity. Remarkably, such techniques have indicated a wide distribution of mostly uncultured archaea in normal habitats, such as ocean waters, lake waters, and soil. This review discusses organisms from the domain Archaea in the context of the environments where they have been isolated or detected. For organizational purposes, the domain has been separated into the traditional groups of methanogens, extreme halophiles, thermoacidophiles, and hyperthermophiles, as well as the uncultured archaea detected by molecular means. Where possible, we have correlated known energy-yielding reactions and carbon sources of the archaeal types with available data on potential carbon sources and electron donors and acceptors present in the environments. From the broad distribution, metabolic diversity, and sheer numbers of archaea in environments from the extreme to the ordinary, the roles that the Archaea play in the ecosystems have been grossly underestimated and are worthy of much greater scrutiny.Key words: Archaea, methanogen, extreme halophile, hyperthermophile, thermoacidophile, uncultured archaea, habitats.
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Shah, Shiraz Ali, Niels R. Hansen, and Roger A. Garrett. "Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism." Biochemical Society Transactions 37, no. 1 (January 20, 2009): 23–28. http://dx.doi.org/10.1042/bst0370023.

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Transcripts from spacer sequences within chromosomal repeat clusters [CRISPRs (clusters of regularly interspaced palindromic repeats)] from archaea have been implicated in inhibiting or regulating the propagation of archaeal viruses and plasmids. For the crenarchaeal thermoacidophiles, the chromosomal spacers show a high level of matches (∼30%) with viral or plasmid genomes. Moreover, their distribution along the virus/plasmid genomes, as well as their DNA strand specificity, appear to be random. This is consistent with the hypothesis that chromosomal spacers are taken up directly and randomly from virus and plasmid DNA and that the spacer transcripts target the genomic DNA of the extrachromosomal elements and not their transcripts.
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Dinkla, Inez J. T., Mariekie Gericke, B. K. Geurkink, and Kevin B. Hallberg. "Acidianus Brierleyi is the Dominant Thermoacidophile in a Bioleaching Community Processing Chalcopyrite Containing Concentrates at 70°C." Advanced Materials Research 71-73 (May 2009): 67–70. http://dx.doi.org/10.4028/www.scientific.net/amr.71-73.67.

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Bioleaching test work was performed in continuously operated multi-stage reactor systems at 70°C using a thermophilic culture treating an Aguablanca Ni-Cu concentrate from Spain and a blend of Cu concentrates from Bor, Serbia. The copper in both these concentrates occurs as chalcopyrite and therefore the use of thermophiles was applied, which resulted in copper recoveries of over 95%. Qualitative assessment of the microbial community in the bioreactors was performed by terminal restriction enzyme fragment length polymorphism (T-RFLP) and clone library analysis of the 16S rRNA genes amplified by PCR. T-RFLP analysis revealed that only archaea were present, and that the communities in both the Aguablanca and Bor systems consisted of two different microorganisms. A 16S rRNA gene clone library using DNA from the Aguablanca system was constructed and screened. Again, two archaea were detected in similar relative abundance in the population as found by T-RFLP analyses. The sequences of these two cloned genes revealed that the dominant archaeon (up to 98% of the total archaea detected) was Acidianus brierleyi, and the other was Metallosphaera sedula. Quantitative assessment of the microbial community was performed by Q-PCR and confirmed the dominance of archaea in the system with Acidianus being the dominant strain (98-99% of the total population) and a minor part of the population (1-2%) consisted of Metallosphaera. Additionally, very small amounts of Sulfolobus spp. were detected. This study, along with other recent studies on the diversity of thermoacidophiles involved in the solubilization of copper from chalcopyrite concentrates, revealed that a wider variety of thermoacidophiles are involved in bioprocessing of metal sulfides, and showed that A. brierleyi should be considered an important biomining acidophile.
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Flores, Gilberto E., Ryan C. Hunter, Yitai Liu, Anchelique Mets, Stefan Schouten, and Anna-Louise Reysenbach. "Hippea jasoniae sp. nov. and Hippea alviniae sp. nov., thermoacidophilic members of the class Deltaproteobacteria isolated from deep-sea hydrothermal vent deposits." International Journal of Systematic and Evolutionary Microbiology 62, Pt_6 (June 1, 2012): 1252–58. http://dx.doi.org/10.1099/ijs.0.033001-0.

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Thirteen novel, obligately anaerobic, thermoacidophilic bacteria were isolated from deep-sea hydrothermal vent sites. Four of the strains, designated EP5-rT, KM1, Mar08-272rT and Mar08-368r, were selected for metabolic and physiological characterization. With the exception of strain EP5-rT, all strains were short rods that grew between 40 and 72 °C, with optimal growth at 60–65 °C. Strain EP5-rT was more ovoid in shape and grew between 45 and 75 °C, with optimum growth at 60 °C. The pH range for growth of all the isolates was between pH 3.5 and 5.5 (optimum pH 4.5 to 5.0). Strain Mar08-272rT could only grow up to pH 5.0. Elemental sulfur was required for heterotrophic growth on acetate, succinate, Casamino acids and yeast extract. Strains EP5-rT, Mar08-272rT and Mar08-368r could also use fumarate, while strains EP5-rT, KM1 and Mar08-272rT could also use propionate. All isolates were able to grow chemolithotrophically on H2, CO2, sulfur and vitamins. Phylogenetic analysis of 16S rRNA gene sequences placed all isolates within the family Desulfurellaceae of the class Deltaproteobacteria , with the closest cultured relative being Hippea maritima MH2 T (~95–98 % gene sequence similarity). Phylogenetic analysis also identified several isolates with at least one intervening sequence within the 16S rRNA gene. The genomic DNA G+C contents of strains EP5-rT, KM1, Mar08-272rT and Mar08-368r were 37.1, 42.0, 35.6 and 37.9 mol%, respectively. The new isolates differed most significantly from H. maritima MH2 T in their phylogenetic placement and in that they were obligate thermoacidophiles. Based on these phylogenetic and phenotypic properties, the following two novel species are proposed: Hippea jasoniae sp. nov. (type strain Mar08-272rT = DSM 24585T = OCM 985T) and Hippea alviniae sp. nov. (type strain EP5-rT = DSM 24586T = OCM 986T).
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Wheaton, Garrett H., Arpan Mukherjee, and Robert M. Kelly. "Transcriptomes of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Exposed to Metal “Shock” Reveal Generic and Specific Metal Responses." Applied and Environmental Microbiology 82, no. 15 (May 20, 2016): 4613–27. http://dx.doi.org/10.1128/aem.01176-16.

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ABSTRACTThe extremely thermoacidophilic archaeonMetallosphaera sedulamobilizes metals by novel membrane-associated oxidase clusters and, consequently, requires metal resistance strategies. This issue was examined by “shocking”M. sedulawith representative metals (Co2+, Cu2+, Ni2+, UO22+, Zn2+) at inhibitory and subinhibitory levels. Collectively, one-quarter of the genome (554 open reading frames [ORFs]) responded to inhibitory levels, and two-thirds (354) of the ORFs were responsive to a single metal. Cu2+(259 ORFs, 106 Cu2+-specific ORFs) and Zn2+(262 ORFs, 131 Zn2+-specific ORFs) triggered the largest responses, followed by UO22+(187 ORFs, 91 UO22+-specific ORFs), Ni2+(93 ORFs, 25 Ni2+-specific ORFs), and Co2+(61 ORFs, 1 Co2+-specific ORF). While one-third of the metal-responsive ORFs are annotated as encoding hypothetical proteins, metal challenge also impacted ORFs responsible for identifiable processes related to the cell cycle, DNA repair, and oxidative stress. Surprisingly, there were only 30 ORFs that responded to at least four metals, and 10 of these responded to all five metals. This core transcriptome indicated induction of Fe-S cluster assembly (Msed_1656-Msed_1657), tungsten/molybdenum transport (Msed_1780-Msed_1781), and decreased central metabolism. Not surprisingly, a metal-translocating P-type ATPase (Msed_0490) associated with a copper resistance system (Cop) was upregulated in response to Cu2+(6-fold) but also in response to UO22+(4-fold) and Zn2+(9-fold). Cu2+challenge uniquely induced assimilatory sulfur metabolism for cysteine biosynthesis, suggesting a role for this amino acid in Cu2+resistance or issues in sulfur metabolism. The results indicate thatM. sedulaemploys a range of physiological and biochemical responses to metal challenge, many of which are specific to a single metal and involve proteins with yet unassigned or definitive functions.IMPORTANCEThe mechanisms by which extremely thermoacidophilic archaea resist and are negatively impacted by metals encountered in their natural environments are important to understand so that technologies such as bioleaching, which leverage microbially based conversion of insoluble metal sulfides to soluble species, can be improved. Transcriptomic analysis of the cellular response to metal challenge provided both global and specific insights into how these novel microorganisms negotiate metal toxicity in natural and technological settings. As genetics tools are further developed and implemented for extreme thermoacidophiles, information about metal toxicity and resistance can be leveraged to create metabolically engineered strains with improved bioleaching characteristics.
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Wheaton, Garrett, James Counts, Arpan Mukherjee, Jessica Kruh, and Robert Kelly. "The Confluence of Heavy Metal Biooxidation and Heavy Metal Resistance: Implications for Bioleaching by Extreme Thermoacidophiles." Minerals 5, no. 3 (July 7, 2015): 397–451. http://dx.doi.org/10.3390/min5030397.

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Rinker, K. D., C. J. Han, and R. M. Kelly. "Continuous culture as a tool for investigating the growth physiology of heterotrophic hyperthermophiles and extreme thermoacidophiles." Journal of Applied Microbiology 85, S1 (December 1998): 118S—127S. http://dx.doi.org/10.1111/j.1365-2672.1998.tb05290.x.

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Dissertations / Theses on the topic "Thermoacidophiles"

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ELIE, CHRISTIANE. "Adn polymerases d'archaebacteries : sensibilite des archaebacteries halophiles a l'aphidicoline : purification et caracterisation de deux adn polymerases chez deux archaebacteries thermoacidophiles." Paris 6, 1989. http://www.theses.fr/1989PA066172.

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Les archaebacteries constituent une troisieme lignee cellulaire intermediaire entre les eubacteries et les eucaryotes; nous nous sommes interesses aux adn polymerases de ces micro-organismes. Nous avons tout d'abord montre que la croissance et la synthese de l'adn in vivo chez des archaebacteries halophiles etaient inhibees par l'aphidicoline, un inhibiteur specifique de la replication chez les eucaryotes. Ce resultat suggere qu'une adn replicase, apparentee aux adn replicases eucaryotes pourrait exister chez ces archaebacteries. J'ai ensuite purifie et caracterise une adn polymerase monomerique, insensible a l'aphidicoline et thermophile chez l'archaebacterie thermoacidophile sulfolobus acidocaldarius. En collaboration avec s. Salhi et j. M. Rossignol, nous avons montre que cette enzyme pouvait repliquer a haute temperature un adn de phage uni-amorce et pouvait etre utilisee pour l'amplification genique par la methode de polymerisation en chaine. Enfin, avec a. Hamal, j'ai purifie et caracterise une autre adn polymerase monomerique, insensible a l'aphidicoline et thermophile chez une autre archaebacterie thermoacidophile phylogenetiquement eloignee de sulfolobus acidocaldarium: thermoplasma acidophilum. J'ai prepare des anticorps polyclonaux diriges contre l'adn polymerase de s. Acidocaldarius et j'ai montre que ces anticorps ne reconnaissaient pas l'adn polymerase de t. Acidophilum
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Williams, Timothy David. "Aspects of lithoautotrophy in iron-oxidizing thermoacidophilic archaea." Thesis, University of Warwick, 1995. http://wrap.warwick.ac.uk/78814/.

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The phylogeny and proteins of chemolithoautotrophic growth of thermoacidophilic Sulfolobus-like archaea were investigated. Sulfolobus-like strain HT is a high growth temperature chemolithotrophic isolate with potential application in mineral sulphide bioleaching. The gene encoding its 16S rRNA was cloned and sequenced. Phylogenetic analysis of this sequence showed strain HT segregating within the genus Sulfolobus. It showed greater similarity to the sequence of Sulfolobus shihatae, a facultative chemolithotroph, than to the sequence of Sulfolobus acidocaldarius, an obligate heterotroph. Flanking regions of the 16S rRNA gene were also sequenced, showing secondary structure similarity to those of S. acidocaldarius, implying a similar excision and processing pathway. A protein of 330 kDa, consisting of 59 kDa and 19 kDa subunits, was overexpressed during CO2-limited autotrophic growth of Sulfolohus strain LM and had previously been shown to co-purify with ATP and acetyl-CoA dependent CO2 uptake. The 59 kDa subunit was partially purified and its N-terminal amino acid sequence obtained. The gene encoding this polypeptide was cloned and sequenced. An open reading frame likely to encode the 19 kDa subunit was adjacent to this gene, forming a possible operon. Homology searches revealed that the predicted amino acid sequence of the 59 kDa subunit was similar to those of ATP-dependent biotin carboxylase enzymes, predicted active site residues being conserved. Homology searches of the predicted amino acid sequence of the ORF likely to encode the 19 kDa subunit revealed similarity to biotin carboxyl carrier proteins, with a biotin binding motif being conserved. In Sulfolobus LM, a polypeptide of 27 kDa molecular weight was overexpressed during autotrophic growth on ferrous iron in comparison with autotrophic growth on tetrathionate. This polypeptide was partially purified and its N-terminal amino acid sequence obtained. After the cloning and sequencing of the gene encoding this protein by a co-worker, homology searches were carried out. It showed homology to the alkyl hydroperoxide reductase / thiol specific antioxidant (AbpCrrSA) family of proteins, members of which are thought to play a role in protection against oxidative stress. The predicted amino acid sequence was phylogenetically analysed, segregating within a group of sequences derived from eukaryotes and archaea, which possess one conserved cysteine residue, as opposed to a group consisting of eukaryotes and eubacteria, possessing two conserved cysteine residues. A membrane bound cytochrome showing a difference spectrum alpha absorbance peak at 572 nm had previously been found to be present only during ferrous iron oxidation in thermoacidophilic archaea. This novel cytochrome was partially purified from the membrane fraction of Sulfolohus strain LM autotrophically grown on ferrous iron. It was shown to retain haem staining activity after SDS treatment, thus allowing its identification as a polypeptide of approximately 66 kDa. A procedure which may allow the N-terminal sequencing of this protein and the initiation of its molecular biological study was identified.
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Giroux, Xavier. "Etude du cycle viral de SSV1, un virus d'archaea thermoacidophile." Paris 11, 2010. http://www.theses.fr/2010PA112214.

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Les Archaea ont été identifiées en 1978 par Carl Woese, séparant le domaine du vivant en trois domaines distinct. Ces organismes ont principalement été isolés à partir d'environnements extrêmes (haute température, haute salinité. . . ). A ce jour, une cinquantaine de virus d'archaea ont été identifiés, et ils présentent une grande variété de morphotypes. Les Fusellovirus, qui infectent les archaea thermoacidophile du genre Sulfolobus, sont parmi les plus étudiés. Les génomes de neuf Fusellovirus ont été séquencés jusqu'à présent, et treize CDS sont communes à l'ensemble de ces virus. Il a été prédit sur la base d'analyses in silico que l'une de ces treize CDS, B251, code un initiateur de réplication de type bactérien. Nous avons montré que B251 possède les caractéristiques d'un initiateur de réplication de type bactérien. Nous avons également identifié une protéine pouvant être impliquée dans l'initiation de la réplication des Fusellovirus. B129 est une protéine à doigt de zinc qui se fixe à la fois sur l'origine de réplication et à proximité du site de recombinaison attP, propriété similaire à celles d'IHF d'E. Coli. En parallèle de ces analyses in vitro, nous avons étudié in silico l'organisation des génomes des Fusellovirus afin de localiser leur origine de réplication. Ces analyses nous ont permis de séparer les Fusellovirus en deux groupes se répliquant potentiellement selon des mécanismes différents. L'ensemble des résultats obtenus participe à une meilleure compréhension du cycle viral des Fusellovirus
Ln 1978, Carl Woese identified a third domain of life, the Archaea. These organisms were first identified in extreme environmental conditions, such as high temperature and high salt concentration. There are about fifty archaeal viruses currently identified, and they have a large variety of morphotypes. Among these viruses, the Fuselloviruses are the best characterized. They infect members of the Sulfolobus genus, isolated from sulfurous hot springs. There are currently nine Fuselloviruses genomes sequenced, with thirteen CDS common to all these viruses. Ln silico analysis predicted that one of these thirteen CDS, B251, could encode a bacterial-like replication initiator. We found that B251 have the characteristics of a bacterial-like replication initiator. We also identified a protein, which could be involved in replication initiation. B 129 is a zinc finger protein wich binds both to the origin of replication and close to the attP site, reminiscent of IHF in E. Coli. We performed in silico analyses of the nine Fuselloviruses genomes to identify their replication origin. Using GC skew analyses, we found that the Fuselloviruses could be divided in two subgroups, each of which replicate their DNA in a different way. All these results help to understand how the Fuselloviruses replicate their DNA and will provide a better understanding of their life cycle
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Han, Chae Joon. "Physiological studies of extremely thermoacidophilic microorganisms undernormal and stressed conditions." Raleigh, NC : North Carolina State University, 1998. http://www.lib.ncsu.edu/etd/public/etd-1052132339841121/etd-title.html.

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Budgen, Nigel. "The catabolism of glucose by the thermoacidophilic archaebacterium Thermoplasma acidophilum." Thesis, University of Bath, 1988. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383178.

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Seipel, Kurtz. "Continuous growth and heat shock of thermoacidophilic Sulfolobus in a triple-stage chemostat for overexpression and isolation of chaperonin." Thesis, University of Iowa, 2012. https://ir.uiowa.edu/etd/2981.

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Smith, Leon David. "Studies of dehydrogenases from thermoacidophilic archaebacteria with a view to cofactor regeneration." Thesis, University of Bath, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328734.

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Helmecke, Julia Verfasser], Dietmar [Akademischer Betreuer] [Schomburg, and Dieter [Akademischer Betreuer] Jahn. "Vom Genom zum systemweiten Verständnis des Stoffwechsels thermoacidophiler Sulfolobales / Julia Helmecke ; Dietmar Schomburg, Dieter Jahn." Braunschweig : Technische Universität Braunschweig, 2019. http://d-nb.info/1198398833/34.

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Angelov, Angel Stoyanov. "Genome sequence analysis and characterization of recombinant enzymes from the thermoacidophilic archaeon Picrophilus torridus." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=974034916.

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Aygar, Sema. "The Role Of Small Heat Shock Proteins Of The Thermoacidophilic Archaeon Thermoplasma Volcanium In The Stress Response." Master's thesis, METU, 2011. http://etd.lib.metu.edu.tr/upload/12613237/index.pdf.

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In this study, possible involvement of the small heat shock proteins (sHsps) from a thermoacidophilic archaeon, Thermoplasma (Tp) volcanium in the stress response was investigated. Our results showed that heterologous, high level expression of TVN0775/sHsp gene in E.coli increased its thermotolerance at 53°
C for two hours. But, the second sHsp of the Tp. volcanium, TVN0984/sHsp was not effective in improvement of the thermal resistance of the mesophilic bacterium (i. e., E.coli). The expression of the TVN0775/sHsp and TVN0984/sHsp genes increased about 3 fold after heat-shock at 65°
C, as revealed by Real-Time PCR analysis. Although expression of the both genes was induced at 70°
C, TVN0984/sHsp gene expression was increased higher (about 5 fold) than that of the TVN0775/sHsp gene expression (about 1.5 fold). Tp. volcanium cells were exposed to high pH (pH: 3.5, pH: 4.0, pH: 4.5, pH: 5.0), and the change in the sHsp genes&rsquo
expression profile were analyzed. The results showed that TVN0775/sHsp gene expression was more sensitive to increased pH than TVN0984/sHsp gene expression. The TVN0775/sHsp gene transcription induced at most 2.5 fold at pH 4.0 and the gene expression either reduced or did not change at higher pH values (i.e., pH 4.5 and 5.0). On the other hand, TVN0984/sHsp gene expression did not change at pH 4.0 but significantly reduced at higher pH values. The effect of oxidative stress on the expression of TVN0775 and TVN0984 genes was investigated by treatment of Tp. volcanium cells with 0.01 mM, 0.02 mM, 0,03 mM and 0.05 mM H2O2. For both sHsp genes, transcription was induced at lower concentrations of H2O2 (0.01 mM and 0.02 mM). At higher concentrations of H2O2 expression of both genes&rsquo
transcription either did not changed or down regulated. Lastly, in this study we have purified the recombinant TVN0775/sHsp, as an Nterminal 6x his-tag fusion to homogeneity on Ni-NTA affinity column. Purified protein samples were used in the chaperone activity assays using bovine glutamate dehydrogenase enzyme (boGDH) as substrate. We have found that the recovery of glutamate dehydrogenase activity at 45°
C, 50°
C and 53°
C in the presence of the Tp. volcanium sHsps was higher than that of spontaneous refolding. Also, TVN0775/sHsp increased the recovery of the boGDH enzyme that was denatured at 2.5 M GdnHCl concentrations for 30 min.
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Books on the topic "Thermoacidophiles"

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Prangishvili. Electrogenic Reactions in Photosynthetic Reactions Centres of Purple Bacteria/Eucaryotic Features of Thermoacidophilic Archaebacteria. Routledge, 1991.

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Book chapters on the topic "Thermoacidophiles"

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Vardanyan, Narine, and Arevik Vardanyan. "Thermoacidophiles for Bioleaching of Copper." In Microorganisms for Sustainability, 177–206. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-3731-5_9.

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Zaparty, Melanie, and Bettina Siebers. "Physiology, Metabolism, and Enzymology of Thermoacidophiles." In Extremophiles Handbook, 601–39. Tokyo: Springer Japan, 2011. http://dx.doi.org/10.1007/978-4-431-53898-1_28.

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Gooch, Jan W. "Thermoacidophile." In Encyclopedic Dictionary of Polymers, 928. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14955.

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Gooch, Jan W. "Extreme Thermoacidophile." In Encyclopedic Dictionary of Polymers, 892. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13727.

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Gulik, Annette, Vittorio Luzatti, Mario de Rosa, and Agata Gambacorta. "Biradical Tetraether Lipids from Thermoacidophilic Archaebacteria." In Advances in Experimental Medicine and Biology, 37–45. New York, NY: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-7908-9_4.

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Maezato, Yukari, Karl Dana, and Paul Blum. "Engineering Thermoacidophilic Archaea using Linear DNA Recombination." In Methods in Molecular Biology, 435–45. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-197-0_26.

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Cubellis, M. V., C. Rozzo, G. Sannia, M. I. Arnone, and G. Marino. "Aspartate Aminotransferase from the Thermoacidophile Archaebacterium Sulfolobus Solfataricus." In Biochemistry of Vitamin B6, 125–28. Basel: Birkhäuser Basel, 1987. http://dx.doi.org/10.1007/978-3-0348-9308-4_21.

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Iwasaki, Toshio, Asako Kounosu, and Sergei A. Dikanov. "The [2Fe‐2S] cluster in sulredoxin from the thermoacidophilic archaeon Sulfolobus tokodaii strain 7, a novel water‐soluble Rieske protein**This investigation was supported in part by Grants‐in‐aid from the Ministry of Education, Science, Sports and Culture of Japan (no. 11169237 to T.I.) and by a grant of Cooperative Research under the Japan‐U.S. Cooperative Science Program from JSPS (BSAR‐507 to T.I.) and NSF (INT‐9910113 to S.A.D.). S.A.D. thanks the Illinois EPR Research Center (NIH grant RR01811) for assistance." In EPR in the 21st Century, 488–93. Elsevier, 2002. http://dx.doi.org/10.1016/b978-044450973-4/50083-4.

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Conference papers on the topic "Thermoacidophiles"

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Kawasaki, Yoko, and Norio Kurosawa. "Construction of a plasmid vector for thermoacidophilic crenarchaeon Sulfolobus acidocaldarius." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0136.

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Schmitz, Rob, Arjan Pol, Sepehr Mohammadi, Carmen Hogendoorn, Ton van Gelder, Mike Jetten, Lena Daumann, and Huub Op den Camp. "The Thermoacidophilic Methanotroph Methylacidiphilum Fumariolicum SolV Oxidizes Subatmospheric H2 with a High-Affinity [NiFe] Hydrogenase." In Goldschmidt2020. Geochemical Society, 2020. http://dx.doi.org/10.46427/gold2020.2310.

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Harris, Carolynn, Jeemin Rhim, Yujiao Zhang, Alec Cobban, Jamie McFarlin, Harpreet Batther, Sebastian Kopf, and William Leavitt. "Determining controls on hydrogen isotope fractionation in archaeal tetraether lipids in a thermoacidophilic archaeal heterotroph." In Goldschmidt2022. France: European Association of Geochemistry, 2022. http://dx.doi.org/10.46427/gold2022.10597.

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You might also be interested in the extended bibliographies on the topic 'Thermoacidophiles' for particular source types:
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