Dissertations / Theses on the topic 'The Stain'
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Strong, Neil. "Fungal deterioration of sawn softwood lumber." Thesis, University of Portsmouth, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285528.
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The colonisation of freshly sawn Corsican pine lumber by sapstain and mould fungi was investigated at a sawmill in Hampshire, UK. Three repeat trials encompassing the different seasons of the year were carried out over two years. Results show that fungal colonisation of sawn lumber is dependent on the effect of time of year. Sawlogs were stored for different intervals up to 16 weeks before conversion to boards. Boards were then stored for up to 12 weeks after milling and sampled every 4 weeks to determine the effect of timber ageing on fungal colonisation up to 28 weeks after felling. The metabolic activity of wood cells over the period after felling of the original tree was also measured. It was evident that the defacement of boards reached maximum levels after 12 weeks exposure irrespective of seasonal influences. Initial levels of fungal growth on lumber were reduced if the boards were milled from logs stored for a period prior to conversion. Investigations into the metabolic activity of the wood cells revealed significant levels of respiration taking place up to 28 weeks after felling of the original tree including 12 weeks post-conversion into boards. Boards were used to make a nested stack arrangement allowing plastic tanks top be placed in the centre. The tanks contained a sub-sample of the full-size boards in order to investigate insect activity and effects of gammairradiation. A total of 115 insect species representing 16 of the 34 British orders were collected during the trials. Seventy-two percent of these insects were collected from within the stacks of lumber and investigations using sealed tanks containing boards showed that the insects could influence the fungal colonisation of sawn lumber. Despite the relatively short length of the trials, a succession of insect colonisation from fungivores through to predators and detritivores was recorded. Boards, which were sterilised by gamma-irradiation, were preferentially colonised by mould fungi and subsequent internal staining was confined to the outer surface. Trials with short-length billets allowed the wood-colonising ability of selected sapstain fungi to be investigated under controlled conditions following sterilisation by gamma-irradiation or autoclaving, and storage at 30°C and 20°C. Lesion formation in gamma-irradiated tissue was solely due to the fungus potentially conditioning the wood for colonisation. Colonisation studies also revealed that different fungi exhibit different strategies enabling them to infect timber. Pathogenic species demonstrated a relatively fast initial growth rate to establish themselves before triggering any host anti-fungal responses in the wood. The characteristic lesions created in the billets were investigated using light and electron microscopy to reveal hyphal invasion and or/ wood cell modifications. Respiratory activity of the lesions was elucidated using radioactively labelled glucose allowing the metabolic pathways to be ascertained and demonstrated that wood tissue in the apparently healthy regions adjacent to the lesions reacted as if infected. Future work considers the possibility of biocontrol, using insects in combination with gamma-irradiation of sawn lumber and also further investigations into the reaction zones produced by the fungus growing in the wood.
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Badcock, Rodney Alan. "Optical fibre sensors for structural stain monitoring." Thesis, Brunel University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389265.
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Yilmaz, Namik Kemal 1975. "A stain-free detection system for electrophoresis." Thesis, Massachusetts Institute of Technology, 2001. http://hdl.handle.net/1721.1/44510.
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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2001.Includes bibliographical references.
In this thesis, a novel stain free detection system for slab gel electrophoresis is examined. Currently, stained techniques are used to identify electrophoretic bands in gels. The stains utilized in these methods involve health risks since they are mutagenic. Also stains like EtBr are intercalating agents meaning they wedge themselves into the grooves of DNA and stay there. Since this includes a physical contact the stains remain in the DNA at the end of the experiment. This makes further use DNA very difficult. The stains need to be removed by chemical techniques which are timewise very costly. Also these operations are very inefficient, retrieve rates are very low which leads to waste of most of the analyte. The specific method we addressed aims to eliminate the use of any kind of stains and therefore inherently increase the end product efficiency. The method introduces the absorption method as the means of detection. The physical law governing the absorption technique is the Beer-Lambert Law. The Beer-Lambert Law defines the linear relation, which correlates absorption value to the analyte concentration, path length of the light and wavelength-dependent absorptivity coefficient. Although the proposed method is intended to apply to all kind of different analytes, to achieve primary goals and prove the feasibility of the method, as the first step detection of DNA molecules are targeted. Hence absorption pattern at a wavelength of 254 nm (which is characteristic absorption peak for DNA) is examined. After the method is proven to work robustly, it will be extended to all kind of different analytes. The unique approach used in the proposed detection system is the use of a scanning technique incorporated with absorption technique utilizing a high QE (Quantum efficiency) CCD camera as the detector. Experiments have been performed to determine the only unknown parameter -wavelength-dependent absorptivity coefficient a([gamma])- in the Beer-Lambert Law. The value of a([gamma]) is dependent on the wavelength and also on the transmission media. In our case wavelength of interest is 254 nm and the specific transmission media is agarose gel with 0.8% concentration. Each lane in the agarose gel is scanned under UV light and transmittance values at 254 nm are recorded as a function of position. The recorded data are processed to see the absorption pattern along the lane. The drop in the signal indicates the existence of a DNA band. Experiments have been performed on three different agarose gels, which are 4 mm thick, and with 0.8% concentration. The value of wavelength-dependent absorptivity coefficient a([gamma]) was determined within an error margin. The resolution of the method was found to be 4 ng/[mu]l.
by Namik Kemal Yilmaz.
S.M.
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Howard, Leigh. "Stain Upon the Silence: Samuel Beckett’s Deconstructive Inventions." TopSCHOLAR®, 1991. http://digitalcommons.wku.edu/theses/1427.
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In recent years, deconstruction theory has emerged as a key method for exploring public address, organizational culture, and literary discourse. Deconstruction theory encourages tearing apart hierarchy and established order to gain insights about the artifact being studied. Furthermore, the theory questions surface or superficial messages and encourages the reader to explore signals hidden below the surface. Deconstruction discounts context and places faith in experience.
Using the early plays of Samuel Beckett, this research explores deconstruction as a method to create messages. This new perspective transports deconstruction from a set of theoretical concepts into basic assumptions that enhance communication. This study suggests that deconstructive inventors use processes previously associated with deconstructive criticism to reveal their own beliefs. Furthermore, this study correlates deconstructive invention with rhetorical tropes – metonomy, synecdoche, metaphor, and irony – to create depiction-based persuasion, which asks the rhetor to suspend logic and evoke emotional response.
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Lister, Thomas. "Simulating the colour of port wine stain skin." Thesis, University of Southampton, 2013. https://eprints.soton.ac.uk/352088/.
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Currently, laser treatments for Port Wine Stain (PWS) lesions are considered the choice therapy, but response is poor or treatments are ineffective for around half of patients. It is proposed in this thesis that improvements to the effectiveness of laser treatment can be achieved through the acquisition of estimated PWS vessel number density, depths and diameters for each individual lesion. Information regarding PWS vessel architecture is found to be contained within the colour of the lesion. Presented in this thesis is a method of extracting this information through colour measurements and the inverse application of a skin model. Colour measurements are performed on 14 participants using a Konica-Minolta CM2600d spectrophotometer employing a xenon flashlamp illumination source and an integrating sphere. Light transport is simulated through an 8 layer mathematical skin model inclusive of horizontal, pseudo-cylindrical PWS blood vessels using a new Monte Carlo programme. Within the programme, model parameters were adjusted in an iterative process and skin colour was reproduced with a mean discrepancy of 1.9% reflection for clinically normal skin (24 datasets) and 2.4% for PWS skin (25 datasets). The programme estimated anatomical properties of the measured regions of skin, yielding epidermal melanin volume fractions from 0.4% to 3.3% and mean melanosome diameters from 41 nm to 384 nm across the participant group. The response to laser treatment was assessed for 10 participants through colour measurements taken immediately before and at least 6 weeks after treatment and through expert analysis of photographs for 9 participants taken at these times. Treatment response was not found to correlate directly with the pre-treatment melanin parameters estimated by the programme. Mean depths, diameters and number densities of PWS vessels were also estimated by the programme before and after treatment. These parameters were compared to data obtained from Optical Coherence Tomography (OCT) images for 5 participants. Number densities and diameters predicted by the simulation varied by no more than 10% from the values determined by OCT for 4 and 5 out of 7 regions respectively. Mean depths predicted by the simulation did not correspond with those determined by OCT however. This may be a result of the limited contribution of deeper vessels to the colour of PWS skin. Predicted PWS parameters were compared to treatment response assessed by colour measurement for 10 participants and by photographic analysis for 9 of these. Predicted vessel number densities were not found to correspond with treatment response. Vessel diameters predicted by the simulation correlated with treatment response when compared with the pulse lengths selected for treatment. Optical coefficients derived from the skin model were used to estimate appropriate laser treatment radiant exposures at the predicted mean vessel depths and these radiant exposures corresponded strongly with the treatment response. Suggestions for improvements in the predictions of melanosome diameters through changes in the adjacent skin minimisation procedure within the programme are discussed. The apparent underestimation of PWS blood vessel number densities and mean depths (compared to biopsy studies) may be a result of the reduced influence of deeper PWS vessels upon skin colour. Further investigation, including modifications to the PWS vessel minimisation procedure within the programme, would be necessary to determine whether improvements in these predictions may be achievable. The results of the study show that the new Monte Carlo programme is capable of extracting, from measurements of skin colour, realistic estimates of PWS skin characteristics which can be used to predict treatment response and therefore inform treatment parameters on an individual PWS.
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Askounis, Alexandros. "Surface nano-patterning using the coffee-stain effect." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/10450.
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Addition of nanopacticles in a base solvent leads to suspensions with enhanced physiochemical properties, compared to base solvent. This new type of suspensions is called nanofluids, with applications ranging from biomedicine to automotives. As a consequence extensive research is being conducted in the field, in particular, on the evaporation of these fluids as it leads to well-defined and highly ordered coffee-rings. However, the exact physics governing this phenomenon remain elusive. The goal of this experimental investigation is to elucidate how various parameters affect the progression of nanofluid coffee-stain formation. Examination of the coffee-ring structuring, produced by the free evaporation of sessile droplets containing nanoparticles, revealed an unexpected, disordered region at the exterior edge of the ring. A self-assembly mechanism with two components, particle velocity and wedge constraints, was proposed to describe the deposition of particles at contact lines of evaporating drops. Environmental pressure was used as a method to control particle crystallinity in the coffee-rings. Essentially, evaporation rate and pressure were found to be inversely proportional. Macroscopically, lowering pressure led to a transition from “stick-slip” to constant pinning. Nanoscopically, lowering pressure promoted crystallinity. Findings supported the proposed, in this thesis, particle self-assembly mechanism. Particle aspect ratio and flexibility were subsequently examined. Pinning strength was found to be a function of particle aspect ratio and rigidity, leading to constant pinning. The proposed, in this thesis, particle self-assembly mechanism was found to be applicable to a variety of aspect ratios and flexibilities. Lastly, particulate crystals grew following different pathways depending on particle flexibility.
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Pepelanova, Iliyana [Verfasser]. "The stain-potential of novel food ingredients / Iliyana Pepelanova." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2012. http://d-nb.info/1023627299/34.
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Powell, John Wellington. "Multiple Stain Histology of Skeletal Fractures: Healing and Microtaphonomy." Scholar Commons, 2015. http://scholarcommons.usf.edu/etd/5835.
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The forensic examination of wounds is one of the key elements of analysis performed by forensic anthropologists and forensic pathologists. Gross examination and histological analysis can be used to determine the timing of the wound and its cause. While forensic pathologists are trained to analyze hard and soft tissue wounds, forensic anthropologists, bioarchaeologists, and paleopathologists, focus on hard tissue. Forensic anthropologists have the added benefit of potentially working with residual soft tissue and would benefit from the incorporation of microscopy techniques that take advantage of the soft tissue to better understand perimortem events. Little research has been published that examines if any healing processes, the defining characteristic of an antemortem wound that do not progress beyond the time of death, are preserved within the tissues beyond death and how long they may be visible.
The objectives of this study were to examine if the use of multiple stains will allow earlier visualization of healing processes in skeletal fractures than gross examination and to observe the length of time microscopic healing structures remain visible after death. A total of 224 slides from 19 specimens representing both fractured and un-fractured bones for control samples were taken from nine autopsied individuals at the Hillsborough County Medical Examiner's Office and analyzed using four stains: Hemotoxylin and eosin (H&E), trichrome, Prussian blue, and elastin stain. Slides were analyzed using a set of 14 scored variables and evaluated with nonparametric statistical tests and cluster analyses. H&E, trichrome, and elastin stains were useful in examining wound age and survival time categories were significantly different for presence of elastin and presence of hemorrhage. H&E and trichrome stains proved useful for observing residual healing structures after death and time cohorts after time of autopsy were significantly different for 11 variables. Results from this study support further testing with larger sample sizes, including samples with a wider range of survival time, to better predict survival times of fractures and time since death.
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Woolfall, Marc P. "Novel mediators for oxidation using hydrogen peroxide." Thesis, University of Liverpool, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367254.
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Koster, Petra Henriette Louise. "Analysis of portwine stain disfigurement and pulsed dye laser treatment results." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2000. http://dare.uva.nl/document/55263.
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Macdonald, Emma Louise. "Laboratory studies into the development and control of extrinsic chlorhexidine stain." Thesis, University of Bristol, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.685431.
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Extrinsic staining of teeth is a common aesthetic problem for which toothpaste manufacturers strive to produce effective products to chemically and/or physically control the problem. Clinical studies proving efficacy are rare and reliance on tests in vitro are more common. The aim of these studies was to develop two in vitro models which could be used to screen and compare agents and formulations for dietary stain control. The first model utilised immobilised hydroxyapatite (RA) with tea as the chromogen. Using residual tea optical density to assess extrinsic staining proved unreliable. Tea extraction and direct measurement from wells however was shown to be reliable in demonstrating the efficacy of known active chemical agents and differentiating between toothpastes. The second model used roughened Perspex to simultaneously assess toothpastes and their ingredients for chemical and or mechanical stain removal. In line with the RA model, results differentiated between toothpastes and other agents for chemical stain removal. Chemical/mechanical stain removal in the model also showed differences for toothpastes, abrasives and detergents but abrasivity levels did not show the expected linear correlation: the toothbrush itself appeared to be the dominant factor in the model. The final experiment attempted to determine whether chromogen/pellicle interaction was specific or non-specific. Results showed no evidence of specificity indeed data suggested that chromogens may merely be absorbed into the pellicle and held physically rather than by chemical interactions. Since some of the data from both models was consistent with other published laboratory and clinical studies it is cautiously concluded that the models may prove rapid, simple and useful screening methods for anti-staining agents and products. Only the availability of more clinical data to support claims for numerous products, however, will allow the models to be retrospectively validated.
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Liu, Jingxin. "Stain separation, cell classification and histochemical score in digital histopathology images." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/52290/.
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This thesis focuses on developing new automatic techniques addressing three typical problems in digital histopathology image analysis, histochemical stain separation at pixel-level, cell classifications at region level, and histochemical score assessment at image level, with the aim of providing useful tools to help histopathologists in their decision making. First, we study a pixel-level problem, separating positive chemical stains. To realise the full potential of digital pathology, accurate and robust computer techniques for automatically detecting biomarkers play an important role. Traditional methods transform the colour histopathology images into a gray scale image and apply a single threshold to separate positively stained tissues from the background. In this thesis, we show that the colour distribution of the positive immunohistochemical stains varies with the level of luminance and that a single threshold will be impossible to separate positively stained tissues from other tissues, regardless how the colour pixels are transformed. Based on this observation, two novel luminance adaptive biomarker detection methods are proposed. The first, termed Luminance Adaptive Multi-Thresholding (LAMT) first separate the pixels according to their luminance levels and for each luminance level a separate threshold is found for detecting the positive stains. The second, termed Luminance Adaptive Random Forest (LARF) applies one of the most powerful machine learning models, random forest, as a base classifier to build an ensemble classifier for biomarker detection. Second, we study a cell-level problem, the cell classification task in pathology images. Two different classification models are proposed. The first model for HEp-2 cell pattern classification comes with a novel object-graph based feature, which decompose the binary image into primitive objects and represent them with a set of morphological feature. Work on cell classification is further extended using deep learning model termed Deep Autoencoding-Classification Network (DACN). The DACN model consists of an autoencoder and a conventional classification convolutional neural network (CNN) with the two sharing the same encoding pipeline. The DACN model is jointly optimized for the classification error and the image reconstruction error based on a multi-task learning procedure. We will present experiment results to show that the proposed DACN outperforms all known state-of-the-art on two public indirect immunofluorescence stained HEp-2 cell datasets and H\&E stained colorectal adenocarcinomas cell dataset. Third, we study an image-level problem, assessing the histochemical score of a histopathology image. To determine the molecular class of the tumour, pathologists will have to manually mark the nuclei activity biomarkers by assigning a histochemical score (H-Score) to each TMA core with a semi-quantitative assessment method. Manually marking positively stained nuclei is a time consuming, imprecise and subjective process which will lead to inter-observer and intra-observer discrepancies. In this thesis, we present an end-to-end deep learning system which directly predicts the H-Score automatically. Our system imitates the pathologists' decision process and uses one fully convolutional network (FCN) to extract all nuclei region, a second FCN to extract tumour nuclei region, and a multi-column convolutional neural network which takes the outputs of the first two FCNs and the stain intensity description image as input and acts as the decision making mechanism to directly output the H-Score of the input TMA image. To the best of our knowledge, this is the first end-to-end system that takes a TMA image as input and directly outputs a clinical score. We will present experimental results which demonstrate that the H-Scores predicted by our model have very high and statistically significant correlation with experienced pathologists' scores and that the H-Score discrepancy between our algorithm and the pathologists is on par with the inter-subject discrepancy between the pathologists.
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Gazzoni, Alexandra Flávia. "Análise descritiva da histopatologia criptocóccica." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2009. http://hdl.handle.net/10183/21435.
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A identificação histopatológica dos agentes fúngicos é um método excelente de diagnóstico, devido ao fato de que as estruturas são facilmente identificadas por meio das técnicas histoquímicas. Até o momento, não dispõem-se de métodos aceitáveis para quantificação da atividade da infecção. Esta investigação protocola um método de estimativa para atividade biológica da criptococose através da determinação dos índice de brotamentos e carminofílico do Cryptococcus. Objetivos: Descrever os aspectos histopatológicos da criptococose através das técnicas histoquímicas básicas e especiais da micologia. Métodos: Foram avaliados 33 pacientes com diagnóstico histopatológico prévio da criptococose. Resultados: Houve predominância do sexo masculino. A idade variou entre 10 a 81 anos, com média de 45,6 anos. A criptococose é doença definidora dos casos de Aids, sendo considerado seu principal fator predisponente, seguido de transplantes. O trato respiratório é o mais envolvido. O microrganismos tem tropismo para o sistema nervoso central e apresenta disseminação para outros órgãos. Resultados falso-negativos é reflexo da deficiência de material capsular. A mortalidade foi de 36%, sendo o maior índice obervado até os 3 primeiros meses após o diagnóstico. A criptococose apresenta-se sob duas formas, reativa e paucireativa. Na infecção reativa, os organismos foram menos abundantes e predominantemente intracelulares aos histiócitos e às células gigantes. Na infecção paucireativa, há um grande número de leveduras, que proliferam-se extracelularmente e estão associadas a destruição do tecido afetado. Ambos infecções, reativas e paucireativas mostraram grande variação no índice de brotamento. O índice carminofílico foi menor nas infecção reativas, quando comparados a infecção paucireativa. Discussão: A coloração de Hematoxilina-Eosina é usada para visualizar as alterações estruturais das lesões, bem como da reação tecidual. A coloração da prata é a mais utilizada para identificação dos organismos fúngicos como o Cryptococcus. A coloração de Mucicarmim de Mayer detecta a cápsula mucopolissacarídica circundante corada na cor magenta. A coloração de FM oferece diagnóstico diferencial nos casos inconclusivos à coloração de Mucicarmim de Mayer. A quantificação de IB e IC é uma escala útil na interpretação da resposta inflamatória do hospedeiro e atividade biológica da criptococose.The histopathologic identification from the fungal agents that's a method excellent of diagnostic, due the fact of what the structures são easily identified for histochemical techniques. So far, there is no methods you accepted about to measurement of biologic activity. This investigation aponta to an method of estimate of the activity biologic of cryptococcal infection by determination of the Budding Index and Carminophilic Index of the Cryptococcus. Objectives: Describes the histologic features of cryptococcosis by basic and special histochemical techniques of mycology. Methods: Have been evaluated 33 patients with previous cryptococcosis histopathologic diagnostic. Results: There is an predominance of the males. The age of the patients ranged from 10 to 81 years with a median value 45,4 years. The HIV infection was the main risk factor for disease, followg of transplants. The respiratory tract is the most frequently involved among the organ systems organs. The false-negative latex test are due to capsular deficiency. The moratlity rate was 36%, The high rate of 50% was observed between of 1 to 3 months. The cryptococcal infections is divided into two major histologic categories, reactive and paucireactive, based upon the host reaction. In reactive infection, the organisms were lessa abundant and were predominantly intracellular within histiocytes and giant cells. In paucireactive infection, thre is large numbers of yeats in the lesions, cryptococci proliferate extracellarly within the involved tissues, associated histologically with mucoid degeneration of the surrounding tissue. Both reactice and paucireactive infections showed great variation in Budding Index. The Carminophilic Inded was lower in the reactive infections, when compared with the paucireactive infection. In this Carminophilic Index presented higher measurements.. Discussion: The Hematoxilin-Eosin stain is used to look for strucutural changes of the infectd lesion, as well tissues reactions. The Gomori’s methenamine-silver stain is the more commonly used in identificatifying these organisms. The mucicarmine stain detecting the surrounding mucopolisacharides capsule of the magenta color. The Fontana-Masson staining offers differential diagnostic. The determination of IB and IC is an scale that relied, because it provides an interpretation of the host response and biological activity of the cryptoccosis.
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Jamieson, Craig. "The development of a "new" stain and its comparison with currently available stains for the evaluation of mycobacteria in processed tissue." Master's thesis, University of Cape Town, 2006. http://hdl.handle.net/11427/3080.
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Nik, Mohamed Nik Elena. "Investigation of blood dynamics : surface flow and droplet stain morphology on fabrics." University of Western Australia. Centre for Forensic Science, 2009. http://theses.library.uwa.edu.au/adt-WU2009.0112.
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This thesis is divided into two parts, each of which examines aspects of bloodstain analysis where gravity is the main force applied to blood. Part I is a preliminary study on the dynamics of blood flow on various inclined surfaces and examines the use of blood analogs for easy test replication. The flow of uncoagulated human blood at different volumes and temperatures was examined on wood at a set angle of 1.5°, and on glass at varying incline angles. Glycerol solutions of 59% and 42% were used to represent blood at 23°C and 37°C respectively. Glycerol flow trials of similar volumes were conducted on wood, PVC and glass. Fluid flow plots of distance versus time exhibited double exponential curve behaviour, although a power-law relationship derived by H. E. Huppert's (1982) flow expression was obtained for blood flowing on inclined wood. Blood flow exhibited several observable characteristics; a decreasing width of the leading edge over time, and streaking and component separation of the leading region at very low speeds. On a glass surface, the width of the initial flow region decreased and initial speed increased with increasing angles. The glycerol analogs used in this study did not represent their blood counterparts well due to differences in physical properties of the fluids. Part II of this study focuses on the forensic value of passive bloodstains on three fabrics; 100% cotton drill, 65/35 polyester cotton, and 100% Shantung silk. 26 µL drops of 37°C human blood were deposited onto the three fabrics and paper from a height of 14 cm at various impact angles. The stains were photographed and analysed qualitatively and quantitatively using computational methods. 100% cotton drill, 65/35 polyester cotton and ironed 100% Shantung silk provided useful forensic values such as direction of travel and angle of impact. Overall, this study has provided useful preliminary data for further research work.
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Sheen, Sarah Rachel. "Laboratory and clinical studies on plaque and stain inhibition on dentures and teeth." Thesis, University of Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.392963.
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SALOMAO, OMAR FERNANDES BRAGA. "LIKE TO WALK AMONG RUINS OR FRAGMENTARY: WHERE STAIN AND LINE ARE CONFUSED." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2018. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=36148@1.
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PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIROCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO
A dissertação é uma tentativa de explorar caminhos de investigação da palavra poética que permitiram sua liberação de formatos estritos, como o processo iniciado por: Um lance de dados jamais abolirá o acaso de Mallarmé; a noção que Haroldo de Campos resgata do poeta-calígrafo, do pensamento do livro total, o livro como um objeto; e a estética da ruína identificada por Walter Benjamin no Barroco alemão. O campo de investigação é o de experiências poético-visuais que interrompem, criam deslocamentos ou obstruções na linguagem: manchas, infiltrações, junções, vestígios, ruínas, cicatrizes, colagens e apagamentos – procedimentos poéticos para permear o inaudito, o indizível, camadas e aberturas no objeto poema. Análise de três casos que se equilibram no limiar do poético e do plástico – Waly Salomão e seus Babilaques; Edgard Braga e seus poemas visuais; e Mira Schendel e suas Monotipias. A escrita da dissertação foi concebida em forma de ensaios e fragmentos, trazendo fotos e imagens-resquícios de cadernos e trabalhos feitos pelo autor ao longo do mestrado. São imagens que procuram ativar outros sentidos, sem obrigação explicativa, expositiva ou informativa, mas com uma relação direta e rizomática com a pesquisa.
The thesis is an attempt to explore ways of investigating the poetic word that allowed their release of strict formats, such as the process initiated by: Un Coup de Dés Jamais N Abolira Le Hasard from Mallarmé; the notion that Harold of Campos rescues from the poet-calligrapher, from the thought of the total book, the book as an object; and the aesthetic of ruins identified by Walter Benjamin in the german baroque. The field of investigation is that of poetic-visual experiences that interrupt, create displacements or obstructions in the language: stains, infiltrations, joints, traces, ruins, scars, collages and erasures –– poetic procedures to permeate the unprecedented, the tacit, layers and gaps in the poem object. Analysis of three cases that balance on the threshold of poetic and plastic –– Waly Salomão and his Babilaques; Edgard Braga and his visual poems; and Mira Schendel and her monotipes. The writing of the dissertation was worked in the form of essays and fragments, bringing photos and imagesremnants of notebooks and works done by me throughout the master s process. They are images that seek to activate other senses, without explanatory, expository or informative obligation, but with a direct and risomatic relation with this research.
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Osooly, Amir. "Development and implementation of robust large deformation and contact mechanics capabilities in process modelling of composites." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/747.
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Autoclave processing of large scale, one-piece structural parts made of carbon fiber-reinforced polymer composite materials is the key to decreasing manufacturing costs while at the same time increasing quality. Nonetheless, even in manufacturing simple flat parts, residual strains and stresses are unavoidable. For structural design purposes and to aid in the assembly procedures, it is desirable to have proven numerical tools that can be used to predict these residual geometrical and material properties in advance, thus avoid the costly experimental trial and error methods.
A 2-D finite element-based code, COMPRO, has previously been developed in-house for predicting autoclave process-induced deformations and residual stresses in composite parts undergoing an entire cure cycle. To simulate the tool-part interaction, an important source of residual deformations/stresses, COMPRO used a non-zero thickness elastic shear layer as its only interface option. Moreover, the code did not account for the large deformations and strains and the resulting nonlinear effects that can arise during the early stages of the cure cycle when the material is rather compliant.
In the present work, a contact surface employing a penalty method formulation is introduced at the tool-part interface. Its material-dependent parameters are a function of temperature, degree of cure, pressure and so forth. This makes the stick-slip condition plus separation between the part and the tool possible. The large displacements/rotations and large shear strains that develop at the early stages of the cure cycle when the resin has a very low elastic modulus provided the impetus to include a large strain/deformation option in COMPRO. A new “co-rotational stress formulation” was developed and found to provide a robust method for numerical treatment of very large deformation/strain problems involving anisotropic materials of interest here.
Several verification and validation examples are used to calibrate the contact interface parameters and to demonstrate the correctness of implementation and the accuracy of the proposed method. A number of comparisons are made with exact solutions, other methods, other experiments and the same models in other commercial codes. Finally, several interesting cases are examined to explore the results of COMPRO predictions with the added options.
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Mohan, Naveen. "Novel diagnostic systems for the assessment of tooth colour and stain in clinical trials." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.491147.
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Tooth whitening is becoming popular in the world today due to peoples' increased awareness of their cosmetic appearance. Tooth whitening is usually achieved either by the physical removal of stain using abrasives or by chemically reducing it with the help of bleaching agents. Product evaluations have been carried out by different investigators and manufacturers to assess the whitening efficacy of different products. Techniques employed in product testing should be standard, reliable, precise and accurate so that meaningful comparisons may be carried out. Tooth whitening and stain has been conventionally assessed by means of visual techniques with the help of shade guides and stain indices. Emergence of instrumental techniques has reduced the subjectivity of conventional techniques and has also provided more standardised methods and parameters for product evaluation. The present clinical studies investigate two novel digital techniques for their suitability to assess and quantify tooth colour and stain. A White Light Digital Imaging system was tested for its capability to assess tooth colour and stain. A novel . Fluorescence Imaging system (Quantitative Light Induced Fluorescence system) was investigated for its suitability to quantify stain. Clinical implementation of a novel whiteness index was also tested for its ability to measure tooth colour and stain. The digital techniques and the parameters were investigated for their ability to assess tooth colour and stain with the help of clinical trials through product evaluation and comparison. The proposed Whiteness Index was concluded as a more appropriate index, amongst the colour parameters, to measure differences in tooth whiteness. The White Light Digital Imaging system was efficient in measuring tooth colour and diffused stain. QLF was concluded as more suitable in detecting and quantifying well defined stain areas on the tooth surface.
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Smart, J. R. "The evaluation of Port Wine Stain Haemangioma before and after treatment by pulsed dye laser." Thesis, University of Strathclyde, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381546.
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Ray, Michael Jason. "The Morphology, Physiology and Molecular Characterisation of the Blue Stain In-service Organism Aureobasidium Pullucans." Thesis, Imperial College London, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.487309.
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Aureobasidium pullulans is generally believed to be the main causal organism of the disfigurement of wood in service and coatings applied to wood. The disfigurement is referred to as blue stain in service. As such it represents a major problem to the paint industry and to the use of timber as a decorative product in service. Fifty two test strains and environmental isolates of blue stain fungi were collated to study the morphology, physiology and phylogeny ofA. pullulans. Sequencing of the rDNA 5.8S gene and flanking ITS regions was successful in unequivocally discriminating between the two morphologically similar blue stain fungi: A. pullulans and H. dematioides. The phylogenetic tree based on the rDNA sequence data confirmed that A. pullulans is a single species and there is no evidence of any subspecies from this region of DNA. RAPD analysis was used to investigate intraspecific genetic variability, but the technique was not successful in being able to differe,uiate accurately between these two fungi. However, different banding patterns were observed, suggesting there was a degree of genetic variability. The sequencing data highlighted many cases of misidentification of H. dematioides as A. pullulans from environmental isolations. The difficulty in correctly distinguishing morphologically between A. pullulans and H. dematioides b!'ought into question the conclusion that A. pullulans was the most commonly isolated and thus the main causal organism of blue stain in service. The actual frequency of occurrence of blue stain fungi in stained wood was investigated using PCR-DGGE analysis of environmental DNA samples. The use ofPCR-DGGE gave an insight into the actual fungi present at the time of sampling and gave a molecular snapshot of the blue stain community present on the stained timber samples. The PCR-DGGE analysis showed conclusively that H. dematioides was detected more frequently than A. pullulans. The results also show that PCR-DGGE, with a suitable primer set, is able to differentiate and partially quantify A. pullulans and H. dematioides in environmental samples. Quantitative analysis of A. pullulans was attempted from a new perspective using direct RNA analysis, in the form ofqPCR, to assess the active biomass ofA. pullulans in stained wood. The results showed the relatively constant levels ofmRNA of the two target genes over the eight week time period of the experiment and demonstrated the effectiveness of the RNA extraction procedure in harvesting extremely small amounts of viable RNA from wood. A method ofcontroIling blue stain fungi was investigated by attempting to reduce the number ofA. pullulans spores adhering to four different wood coatings, namely; a solvent based paint, a water based paint, a solvent based wood stain and a water based wood stain. These coatings were tested both before and after artificial weathering. The inhibition ofspore adhesion follows the tenet of prevention rather than cure and is particularly significant given the current environmental pressures on the coatings industry regarding the use ofbiocides to control these fungi. The main aim of the adhesion study was to examine ifzosteric acid could prevent the adhesion ofA. pullulans spores to wood coatings. Zosteric acid was shown to significantly reduce the adhesive capability ofA. pullulans spores on the four different wood coatings and weathering was shown to significantly increase the adhesion ofspores to the coatings. There is potential for utilising the reduction in spore adhesion observed with zosteric acid by its incorporation into paint formulations. Such an approach has great attraction as zosteric acid is a naturally occurring, non-toxic chemical in contrast to the current use of controlled biocides.
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Kolosova, Natalia. "Transcriptome analysis of conifer defense against bark beetle-associated blue-stain fungi and white pine weevil." Thesis, University of British Columbia, 2010. http://hdl.handle.net/2429/29775.
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Conifer forests are exposed to a large number of herbivorous insect species and pathogenic fungi, some of which cause extensive epidemics and substantial losses of forest resources. Bark beetles and white pine weevil represent major threats to conifer forest health. Bark beetles vector fungal pathogens, which are involved in killing of the host trees. Conifers employ a variety of defense strategies, including anatomical, chemical and molecular defense mechanisms. Recent development of conifer genomic resources and tools including large EST databases and microarrays have allowed for large-scale analysis of conifer defense. To evaluate transcriptome response of conifer species to fungal pathogens I performed a comparative analysis of the interior spruce (Picea glauca x engelmannii) response to spruce beetle-associated pathogenic blue-stain fungus Leptographium abietinum and the lodgepole pine (Pinus contorta) response to mountain pine beetle-associated pathogenic blue-stain fungus Grosmannia clavigera using a 21,843-clone cDNA spruce microarray platform. In addition, I performed a direct comparison of the interior spruce response to inoculation with the fungus Leptographium abietinum with the response to white pine weevil (Pissodes strobi) herbivory.
The microarray analyses revealed substantial changes in the transcriptomes of conifer hosts in response to fungal inoculation or insect feeding with more than a thousand genes significantly differentially expressed in each system and interaction studied. The fungus-induced transcriptomes of spruce and pine shared a large number of similarly responding transcripts with some differences in the dynamics of the induced responses. The transcriptome responses of spruce induced by fungal inoculation and weevil feeding had a large overlap and some treatment-specific trends. Among the most strongly up-regulated transcripts in all interactions were phenylpropanoid pathway transcripts, dirigent protein transcripts, laccases, chitinases and transcripts of the terpenoid pathway. Gene specific expression analysis of selected transcripts confirmed and extended the microarray analysis. Cloning and functional characterization of selected chitinases revealed the presence of chitinolytic activity in two interior spruce and one lodgepole pine class I chitinases. Chitinolytic activity in addition to the strong induction of these chitinases in response to different treatments supported their involvement in conifer defense.
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Perold, Maurits. "The effect of sodium lauryl sulphate on blue stain, mould growth and surface properties of SA pine." Thesis, Stellenbosch : University of Stellenbosch, 2006. http://hdl.handle.net/10019.1/2003.
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Thesis (MScFor (Forest and Wood Science))--University of Stellenbosch, 2006.Producing high quality end products, rather than focusing on volume production, is slowly but surely becoming the main driving force in the wood processing industry of South Africa. Drying defects such as surface checks and discolouration by yellow stain and kiln brown stain are major factors in softwood timber downgrades when selecting furniture grade timber. Previous efforts to control these defects have focused on schedule adaptation, but as the industry is still mainly concerned with volume production, and because of the varying lumber price, longer schedules to control these drying defects have not yet been that attractive for the larger sawmills. In ongoing research in this laboratory, a dip treatment of freshly sawn softwood boards in an aqueous solution containing a surfactant called sodium lauryl sulphate (SLS) was used to try to influence the mechanism involved in the development of these stains. Further, complementary research is reported in this study. Firstly, the possibility of using SLS solutions to control surface mould development and bluestain was investigated. Results showed that SLS did control the development of blue stain and mould growth in open-stacked boards for up to three weeks, using concentrations as low as 0.1%, and up to two weeks in closed-stacked timber when using concentrations of 0.2%. Secondly, it was investigated whether SLS treatment would have a detrimental effect on downstream product quality; in particular, kiln dried boards and glued components for furniture manufacturing. Since SLS influenced fluid water flow during kiln drying, the treatment could have exacerbated the occurrence of surface checking due to altered moisture distribution profiles. Results of this investigation showed that the SLS treatment did not result in increased surface checking. Thirdly, as furniture quality timber treated with SLS would be glued, (and also finished with surface coatings), it was further considered important to determine if SLS treatment influenced adhesion properties of wood surfaces. Based on shear test results, it was established that SLS did not influence the adhesion properties of wood when glued with two most commonly used glues in the furniture industry i.e. polyvinyl acetate (PVAc) and urea formaldehyde (UF).
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Britt, Michelle L. "Culture and identity : the academic setting in Philip Roth's The Human Stain and Francine Prose's Blue angel /." Electronic version (PDF), 2003. http://dl.uncw.edu/etd/2003/brittm/michellebritt.pdf.
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Lubelski, Ryan Edward. "Effect Of Metals And Cetylpyridinium Chloride On Tannin-Protein Interactions: Potential Roles In Extrinsic Teeth Stain Formation." Miami University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=miami1574705098076537.
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Plattner, Alex. "Pathogenicity and taxonomy of fungi associated with the mountain pine beetle in British Columbia." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/345.
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The mountain pine beetle is associated with a diverse array of fungi. Grosmannia clavigera is the most pathogenic of these fungi. A comparison was made between two methods that have been used to assess fungal pathogenicity. Results were similar for older trees inoculated with G. clavigera using either the alternating flap technique or cork borer method. Using the cork borer method, younger lodgepole pine trees were inoculated with five different isolates of G. clavigera. After a 48 week incubation period, isolates ATCC 18086, B5 and H55 had induced stronger pathogenic indicators compared to isolates KW 1407 and B20. After a 7 week incubation period, only isolate ATCC 18086 had induced stronger pathogenic indicators. Usually, this isolate grew faster at lower temperatures and in a low oxygen environment. Isolate KW 1407 consistently produced milder pathogenic indicators during both incubation periods. Among the non-pathogenic fungal associates of the mountain pine beetle, Ceratocystiopsis minuta may be considered the most important because it is the type species for the genus Ceratocystiopsis. The history of this genus is complicated because no physical specimen exists for C. minuta. The phylogeny of the genus Ceratocystiopsis was evaluated. Many isolates of C. minuta were assessed as potential epitypes. Several isolates of C. minuta from previous work were shown to be misidentified. C. minuta isolate CBS 116796 is recommended for future genetic work within the genus Ceratocystiopsis. For morphological work, using measurements from the literature is recommended since CBS 116796 did not produce fruiting bodies.
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Lahoda, Lothar. "Standardisierung und Überprüfung der "Fix vital stain method", einer neuen Färbemethode für Stiersperma mit dem Fluoreszenzfarbstoff Hoechst H33258 /." [S.l.] : [s.n.], 1995. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
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Azevedo, Érika Chang de. "Preparação bioquímica para caracterização molecular e estrutural do RNA vírus LRV1-4." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-27042015-172554/.
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O vírus de Leishmania 1-4 ( do inglês Leishmania RNA virus 1-4 ou LRV1-4) é um vírus da família Totiviridae, e que possui capsídeo icosaédrico e RNA dupla-fita que codifica duas proteínas (proteína capsidial e RNA polimerase). Dados recentes indicam o envolvimento do LRV1-4 na patogênese de Leishmania no hospedeiro humano, tornando seu estudo de fundamental importância para o entendimento dessa doença e de seu papel na relação parasito-hospedeiro. Há relatos sobre a purificação do vírus a partir do seu hospedeiro natural (Leishmania guyanensis) e a partir de sistemas de expressão heteróloga. Este trabalho tem por objetivo estabelecer os métodos de purificação para posteriores estudos estruturais por Microscopia Eletrônica de Transmissão por Contraste Negativo (NS-TEM) e por Crio-Microscopia Eletrônica (Cryo-EM). Os estudos aqui propostos irão permitir a construção de um modelo estrutural do capsídeo do LRV1-4 e sua identificação correta dentre os totivírus. Além das contribuições ao conhecimento da biologia/patogenia do LRV1-4 este estudo representa a primeira caracterização estrutural de um capsídeo viral realizada no Brasil, e assim um avanço importante para a área de virologia e biologia estrutural no pais. Foram realizadas ultracentrifugações biológicas, utilizando gradientes de sacarose, para a purificação do vírus a partir do extrato celular de L. guyanensis. As frações que apresentaram RNA viral foram analisadas por Microscopia Eletrônica de Transmissão (Campinas LNNano CNPEM). Além disso, foram realizadas tentativas de expressar a proteína do capsídeo (ORF2) em Leishmania tarentolae e Escherichia coli. Foram também realizados esforços para a obtenção de anticorpos a partir de peptídeos sintetizados após análise computacional da sequência de aminoácidos da proteína do capsídeo. As amostras obtidas a partir do hospedeiro natural do vírus se apresentaram heterogêneas quando analisadas por NS-TEM, de modo que não foi possível a realização de uma análise estrutural. Porém, a presença de partículas do tamanho esperado para o vírus em amostras em que foi detectado o RNA viral indicam que são necessários esforços para obtenção de uma amostra de maior pureza e homogênea. Além disso, não foi possível obter a proteína do capsídeo nos sistemas de expressão heteróloga. A presença de 25 resíduos de cisteína pode estar levando a proteína à degradação rápida em bactéria. Os experimentos de expressão em células de Leishmania ainda não foram conclusivos. Foi obtido um anticorpo anti-peptídeo que reconhece a proteína do capsídeo, tornando possíveis experimentos como imunolocalização e imunoprecipitação do vírus.The Leishmania RNA virus 1-4 (LRV1-4) belongs to the Totiviridae family. It has an icosahedral capsid and a double-strand RNA encoding two proteins (capsid protein and RNA polymerase). Recent data indicate the involvement of LRV1-4 in the pathogenesis of Leishmania in the human host, making their study of fundamental importance for the understanding of this disease and its role in host-parasite relationship. There are reports on the purification of the virus from its natural host (Leishmania guyanensis) and from the same heterologous expression systems such as Escherichia coli.This work aims to stablish purification methods for further structural studies by Negative Stain Transmission Microscopy (NS-TEM) and Cryo-Electron Microscopy (Cryo-EM). The studies proposed here will allow the construction of a structural model of the coat protein of LRV1-4 and their correct identification amongst the Totiviridae. In addition to the contributions to the knowledge of the biology and pathogenesis of LRV1-4, this study represents the first structural characterization of a viral capsid held in Brazil and thus an important step forward for the field of virology and structural biology in the country. Sucrose for virus purification gradients were performed from the cell extract of L. guyanensis. Fractions that showed viral RNA were analised by Transmission Electron Microscopy (Campinas LNNano - CNPEM). Furthermore, attempts have been made to express the capsid protein (ORF2) in Leishmania tarentolae and Escherichia coli. There has also been made efforts to obtain antibodies from peptides synthesized accordingly to the computer analysis of the amino acid sequence of the capsid protein. The samples obtained from the natural host of the virus showed a heterogeneous distribution of particles when examined by NS-TEM so that it was not possible to perform a structural analysis. However, the presence of particles of the size expected for the virus particles in samples where the viral RNA was detected indicate that efforts are necessary to obtain a more homogeneous and pure sample. Moreover, it was not possible to obtain the capsid protein in heterologous expression systems. The presence of 25 cysteine residues could have led to the protein rapid degradation in the bacteria host. The expression experiments in Leishmania cells were not yet conclusive. It was also possible to obtain an anti-peptide antibody recognizing the capsid protein, enabling immunoprecipitation and immunolocalization experiments.
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Bijelovic, Jelena. "Identification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length Polymorphism." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7100.
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Wood inhabiting fungi oposes a great problem for preservation of wooden surfaces everywhere, being the main problem of economic losses of wooden products.
A reference collection consisting of 9 different genus constituting of 21 different strains of wood-inhabiting fungi was used for identification of unknown species of mould and blue stain fungi on wood. The fungus DNA from the samples was isolated from malt extract agar. PCR (Polymerase Chain Reaction) was conducted on rDNA ITS1 and ITS2 regions for amplification of the DNA. The 21 samples were collected to a reference collection for identification of unknown species of fungi on wooden field samples using PCR and T-RFLP (Terminal Restriction Fragment Length Polymorphism).
PCR-based methods, sequencing and T-RFLP were proven to be simple and
accurate methods for detection and identification of fungi in their early stage.
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Hao, Gang. "Laboratory study of shear wave velocity and very small stain modulus of Macao marine clay under anisotropic stress condition." Thesis, University of Macau, 2008. http://umaclib3.umac.mo/record=b1783644.
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Parker, Nikola Alethia. "The xenogenous capacitation response of fresh, cooled/extended and frozen/thawed equine semen as determined by a chlortetracycline stain /." This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-02132009-171012/.
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Garrison, Haley S. "Effects of Episodic Turbulence on Diatoms: with Comments on the use of Evans Blue Stain for Live-Dead Determinations." W&M ScholarWorks, 2013. https://scholarworks.wm.edu/etd/1539617938.
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Episodic turbulence is a short-lived, high-intensity phenomenon in marine environments produced by both anthropogenic and natural causes, such as boat propellers, strong winds, and breaking waves. Episodic turbulence has been shown to cause mortality in zooplankton, but its effects on marine phytoplankton have rarely been investigated. This study focused on two diatoms: Thalassiosira weissflogii and Skeletonema costatum. I found that exposure for 45 s to turbulence intensities above 2.5 cm2 s-3 caused 24-32% reduction in diatom abundance and increased the amount of intact dead cells to 22%. Turbulence also caused extracellular release of optically reactive DOM. At a turbulence level of 4.0 cm2 s-3, photosynthetic efficiency (Fv/Fm) decreased from 0.51 to 0.38 and 0.55 to 0.50 in T. weissflogii and S. costatum respectively. These turbulence levels are comparable to those under breaking surface waves and are much smaller than those generated by boat propellers.
Despite its relatively short duration, episodic turbulence has the potential to affect phytoplankton via lethal and sublethal effects. An improved technique using the Evans Blue stain was developed to enable visual live/dead plankton cell determinations. When used in conjunction with preservation and flow cytometry, this staining method allows the study of phytoplankton mortality due to turbulence and other environmental stresses.
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Wright, Rebecca. "The stain of colonialism : is educational psychology 'haunted' by the effects of colonialism? : using decolonised methodologies to interrogate practice." Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/20525/.
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This re-search explored how trainee educational psychologists (TEPs) enact educational psychology on their fieldwork placements for the Doctorate in Educational and Child Psychology (DECP). This study seeks to reconstruct educational psychology by exploring oppression, power, resistance, subjugation and revolution in relation to identity politics in educational psychology. Applying a postcolonial theoretical lens of ‘psychopolitics’, this re-search examined how psychological explanations of individual pathology ignore social, political, cultural and economic factors. In light of educational psychology’s history of racialisation and colonialism, the ‘hauntings’ of current methodological tools, narratives and assessments are considered. This re-search moves away from Eurocentric forms of knowledge production in educational psychology, towards radical perspectives from black feminism, critical race theory and decolonised methodologies for ‘knowing’ individuals. The methods autoethnography and sharing circles were used with five Year 3 TEPs to collect stories from their placement experiences. The implications of using decolonised methodologies with white participants who occupy spaces of privilege are also discussed. The ‘knowledges’ gathered from TEPs were interpreted into poetic transcriptions and analysed using a psychopolitical framework. The analysis reveals that educational psychology’s history of measurement, comparison, statistical norms and individual differences informs TEPs’ understandings of their work with children, school staff and families. Educational psychology tends to be discussed in relation to individual descriptions of ‘disorder’, largely neglecting socio-political contexts. The emerging themes include: collusion, power, influence and appropriation. Using decolonised methodologies within a Eurocentric context raises the problem of how invested white participants can be in resistance and revolution. This thesis engages with questions around whether educational psychology can be decolonised and imagined anew. I conclude by arguing that, for change to occur, reform at the individual level of the educational psychologist is essential. Finally, I consider implications for future research and the practice of educational psychology.
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Parker, Nikola A. "The xenogenous capacitation response of fresh, cooled/extended and frozen/thawed equine semen as determined by a chlortetracycline stain." Thesis, Virginia Tech, 1996. http://hdl.handle.net/10919/41023.
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Martins, Cláudio Luís Serra. "Desmanchando preconceitos: a AIDS e a estética da mancha nas peças Angels in America, o Homem e a mancha e a Mancha roxa." Universidade do Estado do Rio de Janeiro, 2009. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=1020.
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O surgimento da AIDS em meados da década de oitenta do século passado traz à tona, mais uma vez, a discussão do binarismo doença e culpa. Para o imaginário dessa época, a mancha estampada no corpo do aidético perde o caráter patológico como causa da ação virulenta do HIV e é substituída por outra mancha que vai revestir não só a pele do doente, mas, principalmente, os olhos acusatórios daqueles que vêem a realidade manchada pelo preconceito. Para tratar desse glaucoma imaginário, abordarei o símbolo da mancha nas três peças que fazem parte de minha pesquisa: Angels in America (1990) de Tony Kushner, O Homem e a Mancha (1997) de Caio Fernando Abreu e A Mancha Roxa (1988) de Plínio Marcos. O objetivo é demonstrar como a mancha é objeto/signo adequado, usado por todos e cada um dos três dramaturgos, na representação do doente de AIDS; para, finalmente, refletir no palco o esboroamento da realidade, através de seu sentido impreciso e sem uma fronteira delimitável. Em O Homem e a Mancha, por exemplo, a personagem, para se libertar da visão dessa mancha estampada em seu corpo, desenvolve um comportamento esquizofrênico, criando um mundo fictício ao seu redor e se isolando do mundo real para viver em sua ilha de solidão. Em Angels in America, o corpo lesionado de Prior Walter provoca mais estupor ao seu companheiro Louis do que ao próprio protagonista. Enquanto Louis tenta fugir da imagem emblemática da culpa, Prior se transforma, não em um marcado para a morte, mas em um escolhido, um novo eleito que desafia o Anjo, pedindo por mais vida. E por último, na peça A Mancha Roxa de Plínio Marcos, diferentemente de Caio e Kushner, o símbolo da mancha aparece de modo mais realista e, acima de tudo, de maneira mais fatalista, pois as presidiárias não estão somente presas fisicamente, mas mentalmente, sem possibilidades para escapismos imagináriosThe appearance of AIDS in the first half of the 1980s brings back to surface the discussion of the illness and guilt binarism. According to the imaginary of that age, the stain stamped on the seropositive body loses its pathological caracter as a virulent action caused by the HIV, replaced by another stain, covering not only the skin of the infected but, principally, the accusing eyes of those who see reality stained by prejudice. In order to treat this imaginary glaucoma, I will analyze the metaphor of the stain in the three plays that make part of my research: Angels in America (1990) by Tony Kushner, The man and the skin lesion (1997) by Caio Fernando Abreu and The Purple stain (1988) by Plinio Marcos. It aims at showing how the stain becomes an appropriate object/sign used by the three playwrights in the representation of the seropositive; and to reflect on stage the blurring of reality, through its imprecise meaning without a delimited frontier. In The Man and the skin lesion, for example, one of the characters, in order to free himself from the vision of a stain stamped on his body, develops a sort of esquizofrenic behavior: he creates a sorrounded fictitious world, provoking his isolation from reality, so that he can inhabit on his solitude island. In Angels in America, Prior Walters skin lesion body causes much more fear to his partner Louis than to the protagonist himself. While Louis tries to escape from the emblematic imagery of guilt, Prior transforms himself not into someone marked to die, but as a chosen one, a new elect, who challenges the Angel asking for more life. And finally The Purple stain presents the metaphor of the stain in a more realistic and fatalistic way: female prisoners are not only physically imprisoned, but mentally, without the possibilities of imaginary escapisms
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Rolland, David. "Nanofluidique de solutions polymériques appliquées à la synthèse in situ d'oligosaccharides." Phd thesis, Université de Grenoble, 2012. http://tel.archives-ouvertes.fr/tel-00721738.
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Les biopuces connaissent un grand essor depuis quelques années avec des applicationspossibles pour l'ADN, les protéines et les oligosaccharides. Une puce à oligosaccharidesprésente des difficultés par rapport à une puce à ADN notamment par les contraintes entempérature et il existe moins de travaux dans ce domaine. Ce travail est donc consacré àl'étude d'une puce à oligosaccharide, par synthèse supportée et par masquage avec un film depolymère. Le procédé de fabrications est particulièrement détaillé.Nous étudions tout d'abord expérimentalement la formation d'un film de polymère obtenu parévaporation d'une goutte de solution polymérique sur une surface structurée chimiquement(zone de mouillabilité différente) en suivant son évolution transitoire. Nous montrons que cetype de surface hétérogène est particulièrement adapté pour la fabrication de biopuces.D'autre part, nous réalisons un modèle numérique de l'évaporation d'une goutte de solutionpolymérique sur une surface chauffée à partir de la méthode de la lubrification et d'un modèlede " hauteur de résine ". Les résultats expérimentaux et de simulation numérique sontcomparés et montrent un bon accord qualitatif sur la forme des films de polymères résultantde l'évaporation.Dans ce travail, la synthèse supportée de biopuces à oligosaccharide est menée à bien enutilisant des polymères et des surfaces judicieusement choisies. En particulier, la technique demasquage par film de polymère se révèle être très bien adaptée pour protéger les oligomères àla fois à hautes et à très basses températures.
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Saczko-Brack, Dario [Verfasser], and Hermann [Akademischer Betreuer] Gaub. "Negative stain electron microscopy and single particle analysis on myosin VI and myosin IX / Dario Saczko-Brack ; Betreuer: Hermann Gaub." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2018. http://d-nb.info/1152946277/34.
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Chiu, Chun-hung, and 趙俊雄. "The role of dynamic cooling in improving clinical efficacy during pulsed dye laser treatment of port wine stain in Chinese." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B26661482.
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Demchuk, David Gregory. "I am Black but in My Heart is No Stain of Infamy: Race Relations in Augusta County, Virginia, 1865-1870." W&M ScholarWorks, 1995. https://scholarworks.wm.edu/etd/1539626001.
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Howard-Jones, Michelle Hope. "Differentiating between living and non-living prokaryotic cells : development, evaluation, and application of a modified vital stain and probe method (mVSP)." Diss., Georgia Institute of Technology, 2002. http://hdl.handle.net/1853/25385.
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Björnsson, Hanna. "Comparison and optimization of May-Grunwald Giemsa and May-Grunwald Giemsa Quick Stain for morphological assessment of pleural and ascites effusions." Thesis, Uppsala universitet, Institutionen för medicinsk cellbiologi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-445869.
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Introduction: Effusion cytology can be performed for the purpose of diagnosis, treatment, and prognosis of malignant disease. A common analysis of effusion cytology samples is the May Grunwald Giemsa stain. Aim: The aim of the study was to compare May Grunwald Giemsa stain and May Grunwald Giemsa Quick Stain in order to determine the best quality stain and suggest ways to improve the current staining protocol. Materials and Methods: The methods used in this study are the routine laboratory’s standard procedures for May-Grunwald Giemsa stain and May-Grunwald Giemsa Quick Stain but with adapted washing steps that investigates the effect of tap water, distilled water, and phosphate buffer on stain quality. Two pleural effusion samples were stained in the initial experiment and two pleural effusions and one ascites sample in the second experiment. Results and Conclusion: All samples gave a greater score when stained with May-Grunwald Giemsa Quick Stain compared to traditional May-Grunwald Giemsa stain. For the traditional May-Grunwald Giemsa, the use of any of the three phosphate buffers scores higher than the routine washing where tap water is used. In conclusion, it would be of benefit to further investigate and implement phosphate buffer in traditional staining or proceed with the May-Grunwald Quick Stain for all pleural and ascites effusions.
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Tao, Jiyue, and Asnaf Aziz. "Simulation of thermal stresses in a disc brake." Thesis, Tekniska Högskolan, Högskolan i Jönköping, JTH. Forskningsmiljö Produktutveckling - Simulering och optimering, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-19163.
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The heat flux produced from the friction between a disc and pad system leads to a high temperature which causes thermal stresses in the disc and after a number of repeated braking cycles, cracks might be initiated. The finite element analysis (FEA) is performed to determine the temperatures profile in the disc and to analyze the stresses for the repeated braking, which could be used to calculate the fatigue life of a disc.Sequentially coupled approach is used for thermo-mechanical problem and the problem is divided into two parts, heat analysis and thermal stress analysis. The heat analysis is obtained by including frictional heat and adopting an Eulerian approach. The heat analysis is conducted by using Abaqus and the toolbox developed by Niclas Strömberg. The thermal stress analysis, which is the main focus of this thesis, is followed using Abaqus. The plasticity theory as background for stress analysis is discussed in detail. The rate independent elasto-plastic plasticity is used in the stress analysis. Temperature independent material properties are considered throughout the thesis work.Isotropic, kinematic and combined hardening models are analyzed for simple 2D academic models for different types of cyclic loads. A benchmark disc and pad model, which is less complicated than the real disc-pad model, is also studied. The linear kinematic hardening model with rate independent elastic-plastic plasticity is used for benchmark and real disc-pad model. The results of the benchmark model and the real model are observed to be similar in terms of plasticity theory.
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Santos, Andrea Barbosa. "Encapsulação de oleoresina de paprica por atomização em goma arabica e em aglomerados porosos de amido/gelatina : estabilidade e aplicação." [s.n.], 2003. http://repositorio.unicamp.br/jspui/handle/REPOSIP/255979.
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Orientador: Carlos Raimundo Ferreira GrossoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: Neste trabalho a oleoresina de páprica oriunda de frutos maduros de pimentões (Capsicum annuum L.), foi encapsulada por processo de atomização, utilizando-se os agentes encapsulantes aglomerados porosos de grânulos de amido de arroz / gelatina e goma arábica. A extração da oleoresina encapsulada foi realizada em ultra-som durante cinco minutos em álcool etílico hidratado apresentando rendimentos de extração de 48,8 e 77,5% para os encapsulados em goma arábica, e aglomerados de amido/gelatina respectivamente. Os rendimentos em relação à retenção durante o processo de encapsulação, corrigidos pela deficiência do método de extração, foram de 100% para cápsulas de goma arábica e em torno de 89% para encapsulados de aglomerados porosos de grânulos de amido de arroz / gelatina, expressos em base úmida. A estabilidade da oleoresina livre e microencapsulada foi avaliada frente às diferentes condições de temperatura (- 18 °C; 25 ± 3°C; 50 °C) e ao efeito da luz, com parte das cápsulas sendo mantidas em ambiente sem iluminação. Em ambos ensaios cápsulas fabricadas com goma arábica apresentaram maior proteção a oleoresina de páprica quanto à perda da cor original. A morfologia da parede mostrou que a parede dos encapsulados de aglomerados porosos de grânulos de amido de arroz / gelatina apresentou porosidade e interstícios foram evidenciados na matriz individualmente e nos aglomerados; em relação as microcápsulas de goma arábica, observou-se parede contínua, sem rachaduras ou poros aparentes, mas apresentando concavidades na superfície provocadas pelo processo de secagem. A distribuição de tamanho das partículas foi unimodal, com tamanho médio de 16,0 mm para as cápsulas de goma arábica e 20,3 mm para os aglomerados porosos de grânulos de amido de arroz / gelatina. A funcionalidade das microcápsulas quanto à liberação do recheio, foi avaliada por adição a dois sistemas alimentícios, um contendo somente gel de gelatina incolor sem sabor e em outro, contendo proteína, gordura, carboidratos (bolo). A distribuição da cor foi homogênea em ambos os sistemas, ou seja, não foi verificado à formação de pontos de concentração de cor nos produtos. A avaliação sensorial foi realizada através do teste afetivo de aceitação em escala hedônica de nove pontos, em relação às formulações dos padrões de gel de gelatina e, de bolo isentas de microcápsulas e do teste de comparação da coloração pela escala estruturada mista. As notas obtidas foram altas para os dois produtos antes da ingestão, média na faixa de seis e sete para a gelatina contendo encapsulados dos aglomerados porosos de grânulos de amido de arroz/gelatina e de goma respectivamente, e média de sete e nove para os bolos
Abstract: In this study the resinous paprika oil obtained from ripe peppers (Capsicum annuum L.), was encapsulated by a spray drying process, in porous agglomerates of rice starch/gelatin and gum Arabic capsules. The process of extracting the encapsulated resinous oil was carried out by breaking the capsules by ultrasonication in a solvent (hydrated ethyl alcohol) for five minutes, showing yields of 48,8 and 77,5% when Arabic gum and starch/gelatin were used respectively. The yields during the encapsulation process with respect to the retention of resinous oil as compared to the amount added, corrected by extractions methods, was 100% for gum Arabic capsules and about 89% when starch granules were formed (wet base). The stabilities of the free and micro encapsulated resinous oils were evaluated under different temperature and light conditions (freezer - 18 °C; room temperature 25 ± 3°C; and 50°C). Exposing part of the capsules in a controlled illumination chamber and maintaining the other part in a chamber with no light tested the effect of light. In both trials (temperature and light), capsules produced with gum Arabic provided greater protection to the paprika resinous oil, with respect to loss of the original color. By morphological observations the porosity of the rice starch was apparent when this material was used as wall material, interstices being observed in the matrix both individually and when agglomerated. When gum Arabic was used as wall material, the walls of the capsules were continuous, with no apparent cracks or pores, although showing some indentations on the surface provoked by the drying process. The average size of the capsules was determined by measuring particle size, both presenting a unimodal distribution, with an average particle size of 16,0 mm for the gum Arabic capsules and 20,3 mm for the rice starch/gelatin. In order to determine the functionality of the microcapsules with respect to the liberation of the core material, the capsules were added to two food systems, a simple system containing non-colored gelatin with no added flavor, and a more complex system containing protein, fat and carbohydrate (cake mix). The color distribution was homogenous in both systems, no localized concentrations of color being noted in any of the products. A preference test with a nine point hedonic scale was used for the sensory evaluation of the gelatin formulations and the cakes, as compared to those containing no microcapsules. The scores for both products were high before tasting, in the range from 6 to 7 for the gelatin containing starch/gelatin and gum capsules respectively, and 7 and 9 for the cakes
Doutorado
Doutor em Alimentos e Nutrição
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Azar, Jimmy. "Automated Tissue Image Analysis Using Pattern Recognition." Doctoral thesis, Uppsala universitet, Bildanalys och människa-datorinteraktion, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-231039.
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Automated tissue image analysis aims to develop algorithms for a variety of histological applications. This has important implications in the diagnostic grading of cancer such as in breast and prostate tissue, as well as in the quantification of prognostic and predictive biomarkers that may help assess the risk of recurrence and the responsiveness of tumors to endocrine therapy. In this thesis, we use pattern recognition and image analysis techniques to solve several problems relating to histopathology and immunohistochemistry applications. In particular, we present a new method for the detection and localization of tissue microarray cores in an automated manner and compare it against conventional approaches. We also present an unsupervised method for color decomposition based on modeling the image formation process while taking into account acquisition noise. The method is unsupervised and is able to overcome the limitation of specifying absorption spectra for the stains that require separation. This is done by estimating reference colors through fitting a Gaussian mixture model trained using expectation-maximization. Another important factor in histopathology is the choice of stain, though it often goes unnoticed. Stain color combinations determine the extent of overlap between chromaticity clusters in color space, and this intrinsic overlap sets a main limitation on the performance of classification methods, regardless of their nature or complexity. In this thesis, we present a framework for optimizing the selection of histological stains in a manner that is aligned with the final objective of automation, rather than visual analysis. Immunohistochemistry can facilitate the quantification of biomarkers such as estrogen, progesterone, and the human epidermal growth factor 2 receptors, in addition to Ki-67 proteins that are associated with cell growth and proliferation. As an application, we propose a method for the identification of paired antibodies based on correlating probability maps of immunostaining patterns across adjacent tissue sections. Finally, we present a new feature descriptor for characterizing glandular structure and tissue architecture, which form an important component of Gleason and tubule-based Elston grading. The method is based on defining shape-preserving, neighborhood annuli around lumen regions and gathering quantitative and spatial data concerning the various tissue-types.
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Meng, Xianzhi. "Assessing the effect of pretreatment on cellulose accessibility for cellulosic biofuels production." Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/54347.
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Biomass recalcitrance has been recognized as one of the major barriers that hided the cost-effective conversion of lignocellulosic biomass to bioethanol, therefore the current bioconversion process require an essential step known as pretreatment to increase the cellulose accessibility. This thesis provides information about changes in cellulose accessibility upon different pretreatments, along with how these pretreatments alter the chemical and physical structures of biomass, will be extremely helpful to further optimize the current pretreatment process. Multiple promising analytical techniques including Simons’ stain, NMR cryoporometry, relaxometry, mercury porosimetry was introduced and successfully applied on pretreated biomass samples to characterize the cellulose accessible surface area and biomass porosity. Different pretreatments increase cellulose accessibility through different mechanisms to different extent. Dilute acid pretreatment is more effective than steam explosion in terms of increasing accessible surface area of cellulose as reflected by Simons’ stain and NMR cryoporometry, while NMR relaxometry suggested steam explosion is more effective at pore expansion for the cell wall water pools detected by changes in NMR relaxation time. Alkaline pretreatment decreased cellulose degree of polymerization, cellulose crystallinity, lignin content and subsequently increased cellulose accessibility, with sodium hydroxide pretreatment proved to be much more effective compared lime or soaking in ammonia pretreatment. Delignification through alkaline-based pretreatment is found less effective than removal of hemicellulose using acid in terms of cellulose accessibility increase. Lignin didn’t directly dictate cellulose accessibility but rather restricted xylan accessibility which in turn controls the access of cellulase to cellulose. Pore size distribution analysis based on mercury porosimetry also indicated that the most fundamental barrier in terms of biomass porosity scale for efficient enzymatic hydrolysis is the nano-pore space formed between coated microfibrils, despite some of the porous architecture such as cell lumen and pit could be severely destroyed after pretreatment. The action of cellulase on the characteristics of cellulosic fractions obtained from pretreated biomass was also investigated. Cellulose accessibility was found to increase at the beginning of hydrolysis, and after reaching a maximum value then starting to decrease. Enzymatic hydrolysis resulted in a rapid decrease in the cellulose degree of polymerization then gradually leveled off, suggesting the existence of a synergistic action of endo- and exo-glucanases that contribute to the occurrence of a peeling off type mechanism.
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Salles, Emily Ferreira. "Feo-hifomicose no Rio Grande do Sul : apresentação de série de casos e comentários sobre o tema em nosso meio." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2010. http://hdl.handle.net/10183/26900.
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Feo-hifomicose refere-se a infecções por fungos pigmentados escuros. Revisamos a casuística brasileira entre 1953 e 2010, apresentando as características clínico-epidemiológicas e diagnósticas de 17 casos. Nas quais a coloração de hematoxilina-eosina foi usada para visualizar alterações estruturais nas lesões; a coloração da prata para identificação dos microrganismos; e coloração de Fontana-Masson confirmou a melanina na parede fúngica. Os fungos cresceram sob aspecto de micélio e produziam pigmentos de melanina, que dão às colônias cor negra característica. A observação das características microscópicas dos cultivos forneceu a identificação etiológica. A feo-hifomicose está amplamente disseminada no Brasil. Entretanto, é subestimada devido a fixação das biopsias em formol, o que impede o isolamento em cultivos.Phaeohyphomycosis refers to infection caused by darkly pigmented fungi. We reviewed the Brazilian casuistic from 1953 to 2010 and presented the clinicalepidemiologic and diagnostic features of addictional 17 cases. In the cases hematoxilin and eosin stain was used to look for structural changes of the infected lesion; Gomori’s methenamine-silver stain identified these organisms; and Fontana-Masson staining confirm the presence of melanin fungal cell wall. The organisms formed mycelial colonies and produced melanin-like pigments that give the colonies the characteristic dark color. The microscopic study of cultures identified etiology. The phaeohyphomycosis is a widespread tropical disease in Brazil. However, it is underestimated due to formalin fixation tissue specimens that oppose the prior cultures.
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Bartsch, Birgit [Verfasser], Roswitha [Akademischer Betreuer] Heinrich-Weltzien, Wolfgang [Akademischer Betreuer] Pfister, and Christian [Akademischer Betreuer] Hirsch. "BLACK STAIN : epidemiologische Untersuchung und quantitativer Nachweis kario- und parodontopathogner Keime mittels Real-time-PCR / Birgit Bartsch. Gutachter: Roswitha Heinrich-Weltzien ; Wolfgang Pfister ; Christian Hirsch." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2011. http://d-nb.info/1016391528/34.
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Persson, Erik. "Storage of spruce pulpwood : effects on wood and mechanical pulp /." Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 2001. http://epsilon.slu.se/avh/2001/91-576-6090-5.pdf.
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Hart, Megan Alexandra. "Evaluation of Stain Blocking Primer Coatings with Low Volatile Organic Compound (VOC) Content and Effects of Drying Conditions on the Properties of Water Reducible Coatings for Polypropylene." DigitalCommons@CalPoly, 2011. https://digitalcommons.calpoly.edu/theses/482.
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The California Air Resources Board (CARB) plans to reduce the volatile organic compound (VOC) limit of specialty primers, sealers, and undercoaters (SPSU), also referred to as stain blocking primers, from 350 g/L to 100 g/L in January of 2012. These coatings are primarily used as stain blocking primers over a variety of substrates, such as wood and drywall. Currently the stain blocking primers that are considered most effective are solvent based primers that contain VOC levels much higher than 100 g/L. The goal of this study is to determine if primers on the market today with a VOC content of 100 g/L or less can provide acceptable performance in comparison to primers with a VOC content greater than 100 g/L. This project had four milestones: 1) survey currently available SPSU coatings and related literature, 2) substrate and stain characterization, 3) benchmark testing of existing formulated commercial products, and 4) prepare a final report.
This report: 1) describes the preliminary work performed in preparation of a more systematic and comprehensive study to evaluate the performance of paints sold for the SPSU market, 2) describes the final results of selected test methods for all primers, and 3) discusses the future steps required for the completion of this project.
In preliminary testing, six primers, both waterborne and solvent based, were selected for testing and comparison. Basic primer characterization was completed, including sag, leveling, contrast ratio, and density, as was preliminary stain blocking testing, including stains by various common markers and tannin staining from dark wood. From the tests conducted during this portion of the project, and through input from an industrial advisory panel, a total of 15 commercially available primers, 7 solvent based and 8 water based, were tested. Acceptable test methods representing both the physical properties and the stain blocking capabilities of the SPSU primers were also selected from the preliminary testing and industry counsel.
As a result of the data obtained from stain blocking testing, conclusions were drawn regarding the stain blocking capabilities of both categories of primer and also about each primer individually. Although it cannot be said that all stain blocking primers, both waterborne and solvent based, perform equally, it can be said that there are waterborne primers that perform as well or nearly as well as solvent based primers in several of the stain blocking tests.
Part B of this report addresses the characterization of a water reducible coating for polypropylene. The solvent retention of the paint with varying drying times and film thicknesses is analyzed using thermogravimetric analysis (TGA). Solvent retention affects the production and shipment of coated polypropylene siding. When solvent is retained within the coating, adhesion can be reduced, and stacking or movement of the product may result in adhesion failure.
Corona treatment of polypropylene was used to increase the surface tension of the substrate and improve adhesion. It was hoped that the surface tension would rise to 60 dynes/cm, however this could not be accomplished. An increase in surface tension to 38 dynes/cm was achieved and proved to be effective at significantly increasing the adhesion of the coating to the substrate.
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Barreto, Michelle Gomes. "Estudo Comparativo das t?cnicas de Imunofluoresc?ncia Direta, Imunofluoresc?ncia Indireta e Colora??o de Sellers em amostra de tecido nervoso de c?es e de animais silvestres para o diagn?stico da cinomose." Universidade Federal Rural do Rio de Janeiro, 2006. https://tede.ufrrj.br/jspui/handle/tede/854.
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Previous issue date: 2006-07-27It was performed a study comparative in the diagnostic of the canine distemper through techniques of Direct Immunofluorescence (IFD), Indirect Immunofluorescence (IFI) and Stain Sellers in samples of nervous tissue of dogs and wild animals that died with suspicion clinical of the viruse. Were utilized 118 samples of central nervous system (SNC) of domestic dogs and 3 wild animals, 2 skunks and 1 monkey, utilizing Ammon?s horn (CA), cerebellum (CB) and brain stem (TE). The TE was the region of larger percentage of positive, following of CA. The comparation between Stain Sellers and IFD, this last demonstrated a large sensitive. The IFI observed what to the realization of the technique was necessary the utilization of fresh materials. The utilization of the 117 samples in conditions of autolysis presented negative result. However 1 sample not autolysate, it was result positive in the 3 brain regions (CA, CB, TE). It was not possible the comparative with the technique of IFI, because in the work the technique alone was possible in materials conserved, with the advantage of a read more easy. In the work was detected also canine distemper in a skunk through technique of IFD.
Foi realizado um estudo comparativo do diagn?stico da cinomose pelas t?cnicas de Imunofluoresc?ncia Direta (IFD), Imunofluoresc?ncia Indireta (IFI) e Colora??o de Sellers em amostras de tecido nervoso de c?es e de animais silvestres que morreram com suspeita cl?nica da virose. Foram utilizados 118 amostras de Sistema nervoso central (SNC) de c?es dom?sticos e de 3 animais silvestres , dois gamb?s e um mico, utilizando-se Corno de Ammon (CA), Cerebelo (CB) e Tronco Encef?lico (TE). O TE foi ? regi?o de maior porcentagem de positivos, seguido do CA. A compara??o entre a Colora??o de Sellers, e a IFD, revelou uma maior sensibilidade nesta ?ltima. Na IFI, observamos que para a realiza??o da t?cnica torna-se necess?rio ? utiliza??o de material fresco. Tendo em vista a utiliza??o das 117 amostras em estado de aut?lise apresentaram resultados negativos e apenas 1 n?o autolisado, teve seu resultado positivo nas 3 regi?es cerebrais (CA, CB e TE). N?o foi poss?vel a compara??o com a t?cnica de IFI, porque nesse trabalho, a t?cnica s? foi poss?vel em materiais conservados, com a vantagem de uma leitura mais f?cil. Nesse trabalho foi detectado tamb?m cinomose em um gamb? pela t?cnica de IFD.