Dissertations / Theses on the topic 'Testis'
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Tao, Lian. "The blood-testis barrier and blood vessel permeability in rat testis." Title page, contents and abstract only, 1994. http://web4.library.adelaide.edu.au/theses/09PH/09pht1712.pdf.
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Bibliography: leaves 150-183. Considers the tubule and capillary barriers from the point of view of anatomy, physiological function and possible factors which may cause the tubule barrier to be breached or influence substance exchange across the capillary wall.
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May, Joel. "Gadd45g and mammalian testis determination." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:6ce4cf97-83da-4cc6-a433-25947f53a7f3.
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Mammalian sex is determined by the presence or absence of Sry. This gene, located on the Y chromosome, promotes the development of a testis from the biopotential gonad by initiating a transcriptional programme that results in Sertoli cell formation. SRY is required to reach a specific threshold level during a critical time-period in order to induce the testicular differentiation pathway. We have previously shown that XY mouse embryos lacking Growth arrest and DNA damage inducible 45 gamma (Gadd45g) exhibit gonadal sex reversal due to a delay in Sry expression. Efforts to study the role of GADD45γ in sex determination have been stymied by the lack of a working antibody. As an alternative, I have produced a BAC transgene produces a similar expression profile to endogenous Gadd45g. This BAC was also shown to rescue the sex reversal found in B6-YPOS gonads, though further research is required to understand the molecular mechanisms through which this occurs. As a result, this BAC was chosen for modification using BAC recombineering to contain the sequence for a poly-His/FLAG epitope tag. Though this edited BAC was generated successfully, no transgenic lines have yet been produced. Members of the Gadd45 family have previously been associated with active DNA demethylation. Assuch, I have conducted whole genome bisulphite sequencing on DNA from XY wild-type, XX wild-type and XY Gadd45g-/- embryos at the 18 tail somite stage, where Sry expression is at its peak. Gadd45g and Sry are only expressed in the somatic cells of the gonads, which have been purified through fluorescence-activated flow cytometry using the transgenic line Sf1-EGFP. Due to the low number of cells that are purified from the embryonic gonad at this stage, sequencing libraries were generated using post-bisulphite adaptor tagging (PBAT). Analysis of the acquired sequences revealed that there are no significant differences between the methylomes of XY wild-type, XX wild-type and XY Gadd45g-/- mutant gonadal somatic cells. It therefore appears that GADD45γ does not have a role in establishing the methylome at this particular stage of sex determination.
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Murphy, Martin P. "Components of MPF in the murine testis." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336742.
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Sweeney, Claire. "Cell cycle regulators in the murine testis." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364276.
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Simpson, B. J. B. "Studies in the Human testis in vitro." Thesis, University of Edinburgh, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383086.
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Su, Linlin, and 苏琳琳. "Drug transporters and blood-testis barrier dynamics." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B47752816.
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Kells, Allan Paul. "Fatty acid elongases of the mammalian testis." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323188.
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Simpson, Barbara Jane Beaton. "Studies on the human testis in vitro." Thesis, University of Edinburgh, 1988. http://hdl.handle.net/1842/30757.
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The obligatory role of pituitary luteinizing hormone (LH) and follicle stimulating hormone (FSH) in the control of testicular function is well established. However there is increasing experimental evidence, largely in the rat, that quantitatively normal spermatogenesis not only requires an adequate local supply of testosterone but also the complex interactions between the various cellular components within the seminiferous tubules and interstitial compartments of the testis. The critical role of paracrine mechanisms in the testis may be reflected by the defective spermatogenesis in idiopathic male infertility where systemic levels of LH, FSH and testosterone are normal or elevated. Thus to further our understanding of the hormonal control of spermatogenesis and to define possible aetiological mechanisms in the infertile man, the study of paracrine mechanisms in the human testis is of paramount importance. The approach to study paracrine mechanisms in the human testis was to establish in vitro techniques whereby individual components of the testis were isolated and specific functional markers defined so that their subsequent interaction could be further studied in vitro. At the same time, the delineation of these 'local' parameters were related to the overall functional states of the testis as defined by circulating levels of LH, FSH, testosterone and the histological assessment of spermatogenesis. Testes were obtained at orchidectomy for prostatic carcinoma and from post mortems carried out within 15 hours of death. Methods were established to examine the effect of intratesticular levels of testosterone and systemic levels of LH, FSH and testosterone on quantitative measures of spermatogenesis. For this purpose a simple technique which involved enumeration of spermatid nuclei in fixed testicular homogenates to determine daily sperm production was adopted. Daily sperm production in this group of ageing testes was generally lower than has been observed previously for younger men. Although intratesticular levels of testosterone varied widely there was no indication of an intratesticular deficiency of testosterone as a critical factor in subnormal spermatogenesis in the ageing testis. Inhibin, a peptide marker of Sertoli cell function was measured in human testicular extracts using a sheep pituitary cell bioassay. Inhibin bioactivity was shown to be present in the human testis. The testicular inhibin content did not seem to bear any relationship to FSH implying that the role of inhibin in the testis may be somewhat different to the classical concept of FSH feedback. A technique for the routine isolation of human Leydig cells was established. Human Leydig cells purified by Percoll density centrifugation were highly responsive to hCG, although sensitivity and receptor number were significantly lower compared to the rat. This system was used to test for the effects of putative paracrine factors on human Leydig cell function. In conclusion, a number of in vitro techniques have been established and validated which provide a basis for future investigation of seminiferous tubule and Leydig cell function in the human testis.
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Freitas, Maria João Martinho de. "Identification of TCTEX1D4 interactome in human testis." Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/9746.
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Mestrado em Biomedicina MolecularT-complex testis expressed protein 1 domain containing 4 (TCTEX1D4) é uma cadeia leve de dineina identificada como sendo uma proteina que interage, no testículo humano, com a fosfoproteina fosfatase 1. As funções específicas da TCTEX1D4 ainda são desconhecidas e identificar as suas proteinas interactoras pode ilucidar sobre as funções desta. Foi aplicado o método de dois híbrido de levedura com o intuito de identificar o interactoma da TCTEX1D4. Foram obtidos 494 clones positivos, dos quais 86 foram identificados correspondendo a 44 diferentes proteinas. Uma análise in silico das características funcionas de todas as proteinas identificadas revelou que as proteinas que interagem com a TCTEX1D4 apresentam funções tão diversas como ligação a iões, ligação ao DNA e actividade peptídica. Também foram obtidas os padrões de expressão em diversos tecidos da base de dados UniGene. Duas proteinas que interagem com TCTEX1D4 são específicas de testículo enquanto 5 são enriquecidas nestes tecido. A rede de interação da TCTEX1D4 foi construída no Cytoscape e combinando os padrões de expressão foi possível identificar possíveis complexos proteicos da TCTEX1D4 específicos ou enriquecidos no testículo. Os complexos TCTEX1D4/TCTEX1D2 e TCTEX1D4/CRISP2 foram caracterizados mais profundamente, revelando que a TCTEX1D4 pode estar envolvida na reação acrossómica, mobilidade do espermatozoide e na interação célula-célula. Em conclusão as funções da TCTEXD4 ainda são desconhecidas mas a identificação e caracterização do seu interactoma ajuda a revelar as suas possiveis funções.
T-complex testis expressed protein 1 domain containing 4 (TCTEX1D4) is a dynein light chain that was identified as a phosphoprotein phosphatase 1 interacting partner in human testis. The specific functions of TCTEX1D4 in testis are still unknown and identification of TCTEX1D4 interacting proteins can ilucidate possible functions of this protein. A yeast two hybrid approach was undertook to identify the TCTEX1D4 interactome. We obtained 494 positive clones from which 86 clones were identified corresponding to 44 different proteins. An in silico analyzis was performed for all proteins identified. In silico functional characterization of TCTEX1D4 interactome reveald its diverse in cellular functions ranging from proteins with ion binding function to DNA binding and peptidase activity. Also a tissue expression distribution was obtained from UniGene database and 2 specififc testis and 5 testis enriched TCTEX1D4 interacting proteins were identified. A TCTEX1D4 network was constructed n Cytoscape and combining tissues expression profiles we were able to identified possble TCTEX1D4 protein complexes specific or enriched in testis. Two TCTEX1D4 protein complexes TCTEX1D4/TCTEX1D2 and TCTEX1D4/CRISP2 were further studied revealing that TCTEX1D4 may be involved in acrosome reaction, sperm motility and cell-cell interaction. In conclusion TCTEX1D4 is still known but identification and characterization of its interactome can help unveil its putative functions.
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Willerton, Louise. "Gene expression in mouse testis during development." Thesis, Connect to electronic version, 2003. http://theses.gla.ac.uk:82/theses/available/etd-07042003-142909/.
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Thesis (Ph. D.)--University of Glasgow, 2003.Ph. D. thesis submitted to the Faculty of Veterinary Medicine, University of Glasgow, 2003. Includes bibliographical references. Electronic version also available via Glasgow University e-Theses service.
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Hess, Monna Fay. "Steroidogenesis in the equine testis throughout puberty /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2003. http://uclibs.org/PID/11984.
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Welborn, Joshua Paul. "Rhox Mediated Actions in the Murine Testis." OpenSIUC, 2014. https://opensiuc.lib.siu.edu/theses/1374.
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The Reproductive Homeobox X-linked, Rhox, genes encode transcription factors that are expressed exclusively in the testis, epididymis, placenta, and ovary. While there are 33 Rhox genes in mice, only Rhox5 and Rhox8 are expressed in Sertoli cells, suggesting that they alone regulate the expression of somatic-cell gene products crucial for testicular metabolism and germ-cell development. Targeted deletion of Rhox5, the founding member of the Rhox gene cluster, in mice, results in decreased expression of the insulin-2 gene (Ins2) and other metabolic genes, male subfertility via reduced sperm number, increased germ-cell apoptosis and a reduced proportion of sperm with normal motility. Davis et al. developed a Rhox8 siRNA knockdown transgenic model to study possible functional similarities between Rhox5 and Rhox8 and reveal compensatory actions via the breeding of Rhox5/Rhox8 double knockout mice. They observed that loss of Rhox8 results in downregulation of the sex-determining region Y gene (Sox9). Further analysis of the role of Rhox5 in testicular metabolism regulation was completed by development of mutant constructs encoding combinations of Rhox5 functional domains and subsequent analysis via qRT-PCR, luciferase assay and immunohistochemistry in cell lines transfected with expression plasmids containing these mutants. Our results indicated direct interaction of RHOX5 with the Ins2 promoter. The homeodomain and amino-terminal domain construct being sufficient for promoter activation albeit at a lower level than the full-length RHOX5 construct. MacLean et al. conducted qRT-PCR analysis of cells transfected with plasmids encoding the other Rhox genes revealed that Rhox8 and Rhox11 were also capable of upregulating Ins2 expression at a lower level than Rhox5. Our analysis of metabolic gene expression in the Rhox8 knockdown model also revealed decreased expression of Ins2 as well as insulin receptor-1 (InsR1). Continued analysis of the Rhox8-KD model and Rhox5/Rhox8 double knockout mice in young and aged (~12 months) male mice revealed a subfertility phenotype characterized by reduced litter frequency and size, reduced total spermatozoa and reduced sperm forward motility. This was reflected by a decrease in RHOX8 and SOX9 protein expression maintained in the aged mice that was more severe in the double knockout animals. qRT-PCR analysis of altered gene expression in the Rhox8 single knockdown male mice revealed significant expression downregulation of fellow Rhox genes Rhox5 and Rhox10 and expression upregulation of the growth differentiation factor-9 (Gdf9) gene normally expressed in the somatic cells of the ovary. Based on the regulation of sex specific genes Sox9 and Gdf9 in the Rhox8 knockdown model as well as data published by Daggag et al. revealing that Rhox8 is the only Rhox member expressed in the somatic cells of the embryonic testis, we sought to elucidate the possible role of Rhox8 in sex determination and testicular differentiation/development. Due to the limitations of our current knockdown model whereby siRNA production is initiated postnatally due to its androgen-dependent promoter, we elected to develop a new Rhox8 knockdown model to characterize the embryonic functions of Rhox8. Rhox8 siRNA were cloned into a Cre-recombinase activated expression vector in which a highly active U6 promoter drives expression of the shRNA. The constitutively active form of this vector exhibited knockdown of Rhox8 in stable Rhox8 overexpressing cell lines developed for this purpose. This developed vector is being used to develop a new transgenic line. While waiting for the development of the new Rhox8 knockdown transgenic mice, we chose to characterize the mRNA and protein expression of Rhox8 in the embryonic testis. Using qRT-PCR and immunohistochemistry, expression of Rhox8 was confirmed and exhibited continually increased expression in the Sertoli cells over the course of embryonic day ~13.5-18.5 (E13.5-E18.5). To examine possible functions of Rhox8 in the embryonic testis prior to receipt of the new knockdown model, we adapted a protocol to transfect the new constitutively active Rhox8 knockdown into embryonic gonads and cultured them up to 72 hours after electroporation. This protocol yielded successful expression of GFP from a GFP-expression plasmid and this was maintained for up to 72 hours in E13.5 gonads. qRT-PCR analysis of gonads transfected with the Rhox8 knockdown expression plasmid revealed significant knockdown of Rhox8 and Sox9 mRNA expression at the 48-hour and 72-hour time points.
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O'Mahony, Orla Ann. "Angiotensin II in male reproduction." Thesis, Queen Mary, University of London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368852.
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Glass, Rainer. "Purinergic signalling in endocrine organs : testis, thyroid, thymus." Thesis, University College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250178.
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Hacker, Adam Michael. "Gene expression during testis determination in the mouse." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283285.
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Milisav-Ribaric, Irina. "Characterisation of human dynein-related genes from testis." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627168.
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Fardilha, Margarida Sâncio da Cruz. "Characterization of the PP1 interactome from human testis." Doctoral thesis, Universidade de Aveiro, 2003. http://hdl.handle.net/10773/4490.
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Doutoramento em BiologiaProtein phosphorylation is a major regulatory mechanism notably of signal transduction cascades in eukaryotic cells. Protein phosphorylation is catalysed by protein kinases and can be reversed by the action of protein phosphatases. Although phosphatases were discovered more than sixty years ago, their importance as central players in multiple cellular mechanisms was only recently recognized. PP1, a Serine/Threonine specific phosphatase, is involved in important cellular mechanisms such as the cell cycle, muscle contraction and apoptosis, among others. Its role in such diverse cellular processes depends on its interactions with targeting/regulatory subunits. To date, more than 50 regulatory subunits have been identified that bind the catalytic subunit of PP1 determining its function in a specific cellular location. Several isoforms of PP1 are known, termed PP1 , PP1 and PP1 . The gamma isoform undergoes alternative splicing to yield a ubiquitously expressed PP1 1 and a testis-specific PP1 2 isoform. Incubation of non-motile immature sperm with phosphatase inhibitors induces sperm motility, and PP1 2 was implicated in this process. This led us to search for PP1 2-specific interactors in human sperm that could be targeted for infertility therapeutics or in male contraception. To achieve this goal the Yeast Two Hybrid system was used to screen a human testis library for new PP1 binding proteins using both PP1 1 (YTH1) and PP1 2 (YTH2) as baits. We recovered 120 positive clones in YTH1 and 155 positive clones in YTH2. Among these were clones encoding “bona fide” PP1 interactors such as Nek2 and NIPP1, and also previously uncharacterized proteins. We undertook a more detailed study of a novel gene encoding a novel protein that we termed SEARP-T. This protein of 93KDa is expressed mainly in testis and fluorescence immunocytochemistry was used to determine its intra sperm localization. Both PP1 2 and SEARP-T proteins are present in the tail and in the equatorial segment of the head.These results provide new insights into PP1 function in human testis and sperm motility, and indicates that the Yeast Two Hybrid System provides a mean to understand the roles PP1 plays in diverse cellular regulatory events.
A fosforilação de proteínas é um dos principais mecanismos reguladores de cascatas de transdução de sinais em eucariotas. A fosforilação é catalizada por proteínas cinases e é revertida pela acção de proteínas fosfatases. Embora as proteínas fosfatases tenham sido descobertas à mais de sessenta anos, a sua importância central em múltiplos mecanismos celulares só muito recentemente foi reconhecida. PP1, uma fosfatase específica para serina/treonina, está envolvida em importantes mecanismos celulares, como o ciclo celular, a contracção muscular e a apoptose, entre outros. O seu papel em tão variados processos celulares depende das suas interacções com subunidades reguladoras. Até à data, foram descritas mais de 50 subunidades reguladoras que se ligam à subunidade catalítica da PP1, sendo determinantes para a sua função num local específico da célula. Das três isoformas da PP1 conhecidas, PP1 , PP1 e PP1 , a isoforma gama sofre splicing alternativo, originando a PP1 1, ubíqua e, a PP1 2 específica de testículo. Incubação de espermatozóides imaturos com inibidores de fosfatases induz a sua motilidade, sendo a PP1 2 a fosfatase envolvida. Este facto levou-nos a procurar proteínas de testículo humano capazes de interagir especificamente com a PP1 2 que possam ser alvos terapêuticos no tratamento da infertilidade, ou na contracepção masculina. Para atingir este objectivo utilizou-se o sistema Dois Híbrido de Levedura no rastreio de uma biblioteca de testículo humano, na busca de novas proteínas que se ligam à PP1 usando como isco a PP1 1 (no YTH1) ou a PP1 2 (no YTH2). Obtivemos 120 clones positivos no YTH1 e 155 no YTH2. Entre eles encontravam-se clones que codificam reguladores da PP1 previamente descritos, como a Nek2 e a NIPP1, assim como novas proteínas. Efectuámos um estudo detalhado de um novo gene, codificando uma nova proteína, que denominamos SEARP-T. Esta proteína de 93kDa é expressa maioritariamente em testículo e, a sua localização intracelular foi determinada por imunocitoquímica de fluorescência. Ambas as proteínas, PP1 2 e SEARP-T, estão presentes na cauda e no segmento equatorial da cabeça do espermatozóide. Estes resultados clarificam as funções da PP1 no testículo humano e na motilidade do esperma e, indicam que o sistema Dois Híbrido de Levedura é um bom método para compreender o papel da PP1 em múltiplos eventos de regulação celular.
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Clement, Tracy M. "Molecular mechanisms of sex determination and testis differentiation." Pullman, Wash. : Washington State University, 2009. http://www.dissertations.wsu.edu/Dissertations/Spring2009/t_clement_050709.pdf.
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Slider, Cara L. "Encouraging testicular self-examination behaviors in college males examining the role of fear appeals in protection motivation theory /." Morgantown, W. Va. : [West Virginia University Libraries], 2009. http://hdl.handle.net/10450/10148.
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Thesis (M.S.)--West Virginia University, 2009.Title from document title page. Document formatted into pages; contains iii, 76 p. : ill. Includes abstract. Includes bibliographical references (p. 40-43).
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Pfeiffer, David Carl. "Actin associated intercellular adhesion junctions in the mammalian testis." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29753.
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In the mammalian seminiferous epithelium, the cytoplasm of Sertoli cells adjacent to sites of intercellular attachment exhibits unique structural attributes. In each of these regions, a layer of hexagonally packed actin filaments lies situated between a cistern of endoplasmic reticulum and the plasma membrane. The filament layer together with the reticulum and adjacent plasma membrane are collectively termed an "ectoplasmic specialization". Ectoplasmic specializations occur in apical Sertoli cell regions at sites of attachment to spermatids and basally at sites of attachment to adjacent Sertoli cells.
Ectoplasmic specializations have been hypothesized to be actin associated intercellular adhesion junctions. If this is true, molecular components that characterize actin associated adhesion junctions in general should be present in ectoplasmic specializations. In this study, I tested this prediction in two ways. First, I investigated whether or not the protein vinculin is co-distributed with actin filament bundles in ectoplasmic specializations of the ground squirrel. Second, I immunologically probed ectoplasmic specializations for three cell adhesion molecules (CAMs) that are commonly found in regions of intercellular adhesion in other tissues. My results indicate that vinculin is co-distributed with actin in Sertoli cell regions attached to spermatids. These data are consistent with the conclusion that vinculin is a component of ectoplasmic specializations and, therefore, with the hypothesis that the latter structures are a form of actin associated adhesion junction. Experiments using probes for the CAMs indicate that E-cadherin, A-CAM and N-CAM are probably not present in ectoplasmic specializations. The adhesion molecule at these sites may be a different member of the known CAMs or an as yet unidentified CAM.
Based on data presented here and elsewhere indicating that ectoplasmic specializations are a form of actin associated adhesion junction, I describe the elaborate changes that occur in constituent filament bundles at sites of attachment to spermatids of the ground squirrel and interprete them in the context of the adhesion hypothesis. During the course of the co-localization studies described above, I observed that vinculin and actin are co-distributed at certain sites of intercellular attachment between interstitial cells of Leydig in the ground squirrel testis. Moreover, at the ultrastructural level I found these sites correspond to microfilament rich junction regions. These observations are consistent with the conclusion that actin associated intercellular adhesion junctions exist between interstitial cells of Leydig in the ground squirrel testis.Medicine, Faculty of
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莫穎兒 and Wing-yee Bobo Mok. "Expression of Gap-junctional connexin 31 in rat testis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1999. http://hub.hku.hk/bib/B31223205.
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Mok, Ka-wai, and 莫嘉維. "Blood testis barrier: its biology and significance in spermatogenesis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B49799502.
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Spermatogenesis takes place in the seminiferous epithelium and it is a tightly regulated process that produces spermatozoa from spermatogonia. During spermatogenesis, germ cells have to traverse the seminiferous epithelium, from basal to adluminal compartment and finally reach the luminal edge of the seminiferous tubules at spermiation. During the transit of germ cells, they have to get across the blood-testis barrier (BTB), which is formed by adjacent Sertoli cells. Thus, although BTB is considered as one of the tightest blood-tissue barrier, the BTB undergoes cyclic restructuring to “open” transiently for the translocation of germ cells. However, the integrity of the BTB has to remain intact as the BTB is essential for the developing germ cells behind the barrier. For example, the BTB serves as an immunological barrier to “seal” developing germ cells from the systemic circulation. Since how the BTB restructuring is regulated remains elusive, the study herein aims to provide some information regarding to this events.
The importance of the BTB to spermatogenesis was demonstrated by treating rats with 50 (lowdose) or 250 mg/kg b.w (high-dose) of adjudin. Although the BTB of rats was perturbed in both groups at week 6 post treatment, as shown by an in vivo BTB functional assay, the BTB of the low-dose group was found to have “resealed” at week 20 whereas the BTB of the high-dose group remained disrupted. Besides, despite almost all germ cells were depleted in both group of rats upon week 2 post treatment, spermatogonia were still present in the testis of rats no matter high- or low-dose of adjudin was used. However, spermatogenesis only recovered in low-dose treated group, which have an intact BTB. This suggests that after spermatogenesis is disrupted, its regeneration of spermatogenesis needs more than the existence of spermatogonia in which an intact BTB is required. After demonstrating the necessity of the BTB for spermatogenesis, the next question I addressed was how its restructuring was modulated. The involvement of mammalian target of rapamycin (mTOR) in manipulating the BTB was investigated. mTOR is able to form two distinct signaling complexes namely mTOR complex 1 (mTORC1) or mTORC2 by assembling with raptor or rictor, respectively. rpS6, which is a downstream molecule of mTORC1 was activated specifically during BTB restructuring and knockdown of rpS6 in cultured Sertoli cells was able to promote the TJ-barrier by inducing deposition of F-actin and BTB proteins at the cell-cell interface, suggesting the role of phosphorylated rpS6 is to “open” the BTB for the transit of spermatocytes. Moreover, the knockdown of rictor led to perturbation of TJ-barrier formed by cultured Sertoli cells via a PKC-α depending actin reorganization, causing internalization of BTB proteins. This indicates mTORC2 is necessary for the maintenance of the BTB and hence the two mTOR complexes work antagonistically to regulate the BTB in which mTORC1 is activated to promote the BTB restructuring while the expression of mTORC2 is essential to sustain the BTB integrity.published_or_final_version
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Connor, Frances Ann. "Analysis of Sry-related genes expressed in mouse testis." Thesis, Institute of Cancer Research (University Of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309162.
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Pagotto, Anna. "Functional analysis of cancer/testis antigens in human cancer." Thesis, University of Oxford, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.711647.
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Mok, Wing-yee Bobo. "Expression of Gap-junctional connexin 31 in rat testis /." Hong Kong : University of Hong Kong, 1999. http://sunzi.lib.hku.hk/hkuto/record.jsp?B21254084.
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Zabida, Omer Saleh. "The effect of methamphetamine on the blood-testis barrier." University of the Western Cape, 2018. http://hdl.handle.net/11394/6775.
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>Magister Scientiae - MScIntroduction The blood-testis barrier (BTB) is formed by tight junctions between adjacent Sertoli cells. The barrier formed by these tight junction helps to create a specialized environment for spermatogenesis and provide an immunological barrier to protect developing germ cells. Methamphetamine (Meth) is known as neurotoxin however, its effects on the male reproductive system, especially on Sertoli cells and, the BTB are not well established. Therefore, this study aimed to determine the effects of Meth on the TM4 mouse testis Sertoli cell line and on the integrity of the BTB permeability. Materials and Methods This study investigated the effect of selected concentrations of Meth (0.1 μM, 1 μM, 10 μM, 20 μM and 100 μM) on TM4 mouse testis Sertoli cell line for 24 until 96 hours, using two treatments: an “acute” study (24 hrs exposure) and a “chronic” study, where treatment occurred on a daily basis over 96 hrs. The following parameters were investigated: viability, cell proliferation, mitochondrial activity, monolayer permeability.
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Papp, Steven Ray. "Immunocytochemical localization of glutathione S-transferase subunits in the testis, rete testis, efferent ducts and epididymis of the adult rat and during postnatal development." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55521.
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In the present study, immunolocalization of several glutathione S-transferase (GST) subunits in the testis and excurrent duct system of the adult rat as well as during postnatal development was performed. Dimeric GSTs come from a multigene family and their subunits can be grouped into five classes $( alpha, mu, pi, zeta, theta)$ based on amino acid homology. These isozymes catalyze the conjugation of glutathione with a wide variety of electrophiles, thereby protecting important cellular constituents from electrophilic attack. Light microscope immunocytochemistry using antibodies raised against the Ya and Yc subunits of the alpha class and the Yb$ sb1$ and Yb$ sb2$ subunits of the mu class revealed the distribution. The variability and immunoreactivity to the various subunits of GSTs in the epithelial cells of the extratesticular ducts indicate a marked evolution of the potential role in the detoxification of electrophiles during development and a specialization of this function in the various types of cells along the epididymal epithelium in adult animals.
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Hollenback, David. "Properties of a CoA-dependent, stearoyl-specific transacylase from bovine testis membranes /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/9219.
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Kokk, Kersti. "Regulation of active and passive molecular transport in the testis /." Online version, 2005. http://dspace.utlib.ee/dspace/bitstream/10062/1305/5/kokk.pdf.
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Santos, Lopes Bruno Miguel. "Cancer-Testis Antigene als neue prognostische Faktoren für das Plasmazellmyelom /." [S.l.] : [s.n.], 2009. http://opac.nebis.ch/cgi-bin/showAbstract.pl?sys=000292615.
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Pratis, Kyriakos 1973. "Hormonal regulation of 5α-reductase isoforms in the rat testis." Monash University, Dept. of Obstetrics and Gynaecology, 2001. http://arrow.monash.edu.au/hdl/1959.1/9184.
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Benson, Elizabeth Anne. "Patterns and regulation of gene expression in the Drosophila testis." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.432553.
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Chan, Tung-lei, and 陳冬妮. "Promoter characterization of testis specific protein, Y-linked like2 (TSPYL2)." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41290410.
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Li, Wing-man Michelle, and 李穎雯. "Gap junction regulates the blood-testis barrier dynamics during spermatogenesis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45450225.
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Lam, Hang-yee Chloe, and 林倖而. "Identification of interacting partners of LFA-1 in the testis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/202255.
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Holland, Beverley. "The molecular genetics of germ cell tumours of the testis." Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389269.
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Klein, David Michael. "Nucleoside and HIV Drug Transport at the Blood-Testis Barrier." Diss., The University of Arizona, 2015. http://hdl.handle.net/10150/581323.
Full textAbstract:
The immune-reactive sperm are kept separate from the body by epithelial barriers such as the blood-testis barrier (BTB). While these barriers are beneficial for the protection of sperm from toxicants, they can make treating these areas difficult due to preventing the entry of pharmacological agents. This is especially an issue in the treatment of HIV and Ebola infection based on the ample evidence that these viruses are able to survive and spread from within the male genital tract (MGT), but only a few antiviral drugs are known to access the MGT. Transporters that line the epithelial barriers of the MGT, especially the BTB, are important for determining whether or not a drug is able to penetrate into the MGT through transepithelial transport. Several nucleoside analogs (NSA), which are used to treat HIV infection and leukemias, are known to be able to accumulate in seminal plasma, which makes them a useful tool for understanding transepithelial transport for the BTB. The purpose of these studies is to characterize the transport profile for the MGT, in particular the BTB, to gain a better understanding of how xenobiotics, especially ones based on nucleosides, can access the MGT. The chief finding of this work is the discovery of a transepithelial transport pathway expressed by Sertoli cells that allows for the entry of nucleosides (necessary for germ cell development) and NSA into the MGT. This pathway depends on equilibrative nucleoside transporter (ENT) 1 uptake and ENT2 efflux and occurs in both rats and humans. These studies provide the foundation for being able to predict the penetration of novel drugs into the MGT.
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Phochanukul, Nichanun. "Genomic analysis of Drosophila Sox100B during embryogenesis and testis development." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609213.
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Sousa, Solange Damasceno. "ProteÃmica do fluido da Rete Testis de carneiros Morada Nova." Universidade Federal do CearÃ, 2015. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13842.
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FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgicoO objetivo do estudo foi identificar e caracterizar as proteÃnas do fluido da rete testis de carneiros Morada Nova. Os testÃculos, obtidos de seis carneiros Morada Nova abatidos, foram imediatamente dissecados. A cabeÃa do epidÃdimo foi separada para ter acesso aos ductos eferentes. O fluido oriundo dos ductos eferentes foi obtido por massagem do testÃculo. Posteriormente, o fluido foi centrifugado para remoÃÃo dos debris celulares e espermatozoides. As proteÃnas foram precipitadas com acetona a -20ÂC e quantificadas pelo mÃtodo de Bradford. Cada amostra (400 μg) foi focalizada em tiras de 13 cm (pH 4-7) e a segunda dimensÃo foi realizada em SDS-PAGE a 15%. Os gÃis obtidos foram escaneados com um ImageScanner II (GE Lifesciences, EUA) e analisados utilizando o aplicativo PDQuest versÃo 8.0.1 (Bio-Rad Laboratories, EUA). Os spots detectados apÃs a anÃlise dos mapas 2-D no PDQuest foram cortados dos gÃis e submetidos a digestÃo com tripsina. As proteÃnas foram identificadas por espectrometria de massa em tandem. A informaÃÃo sobre a proteÃna adquirida pela busca no MASCOT foi analisada atravÃs da utilizaÃÃo do programa para anotaÃÃes de proteÃnas (STRAP). Redes de interaÃÃo proteÃna-proteÃna foram obtidas a partir do banco de dados STRING versÃo 9.0. Nos gÃis foram detectados em mÃdia 227  32,1 spots, onde 51% das proteÃnas se encontraram acima de 40 kDa representando 65% da intensidade de todos os spots detectados nos gÃis. Com base na anÃlise da ontologia gÃnica, os processos biolÃgicos mais comuns associados Ãs proteÃnas do fluido rete testis foram regulaÃÃo (24,28%) e processos celulares (23,27%). LigaÃÃo (27,42%) e a atividade catalÃtica (19,30%) corresponderam Ãs funÃÃes moleculares mais frequentes. As proteÃnas mais intensamente expressas foram: albumina, clusterina, serotransferrina, imunoglobulina gama-1 e alfa-2-HS-glicoproteÃna. A maioria das proteÃnas identificadas desempenha importantes papÃis nos processos de espermatogÃnese, proteÃÃo espermÃtica, motilidade, capacitaÃÃo e reaÃÃo acrossÃmica. O fluido da rete testis possui vÃrias proteÃnas envolvidas na espermatogÃnese, o que representa um importante fator, uma vez que estas molÃculas contribuem para o desenvolvimento das cÃlulas germinativas, participando no transporte e conversÃo de substÃncias requeridas para a produÃÃo dos gametas masculinos.
The aim of the study was to identify and characterize the proteins of the rete testis fluid from Morada Nova rams. The testicles, obtained from six slaughtered Morada Nova rams, were immediately dissected. The head of the epididymis was separated to gain access to the efferent ducts. The fluid from the efferent ducts was obtained by testis massage. Thereafter, the fluid was centrifuged to remove cell debris and sperm. Proteins were precipitated with acetone at -20ÂC and quantified by the Bradford assay. Each sample (400 Âg) was focused in strips of 13 cm (pH 4-7) and the second dimension was conducted on SDS-PAGE 15%. The gels were scanned with an ImageScanner II (GE Lifesciences, USA) and analyzed using the PDQuest version 8.0.1 (Bio-Rad Laboratories, USA). Spots detected after PDQuest analysis of 2-D maps were cut from gels and submitted to trypsin digestion. Proteins were identified using tandem mass spectrometry. Protein information obtained by MASCOT was analyzed using the software tool for searching annotations of proteins (STRAP). Protein-protein interaction networks were obtained from STRING version 9.0 database. In the gels were detected 227  32.1 spots (mean  SD), where 51% of the proteins were found above 40 kDa, corresponding to 65% of the intensity of all spots detected. Based on gene ontology analysis, the most common biological processes associated with proteins from rete testis fluid were regulation (24.28%) and cellular process (23.27%). Binding (27.42%) and catalytic activity (19.30%) corresponded to the most frequent molecular functions for those proteins. The most intensely expressed proteins were: albumin, clusterin, serotransferrin, immunoglobulin gamma-1 chain and alpha-2-HS-glycoprotein. The rete testis fluid has a large quantity of proteins related to the spermatogenesis. This feature is important in view of the fact that these molecules contribute to the development of the germ cells, as a result of the transport and conversion of substances required to the production of male gametes.
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Silva, Joana Vieira da. "Electron microscopy study of PP1 and SARP2 in human testis." Master's thesis, Universidade de Aveiro, 2011. http://hdl.handle.net/10773/7698.
Full textAbstract:
Mestrado em Biomedicina MolecularA proteína fosfatase-1 (PP1) é uma fosfatase específica para a desfosforilação de resíduos de serina e treonina, cujas funções celulares dependem da formação de complexos com proteínas que interagem com a PP1 (PIPs). Dada a importância da PP1γ2, isoforma específica de testículo/espermatozóides, na espermatogénese e na função dos espermatozóides, é fundamental identificar as funções das PIPs específicas destes tecidos, tais como a SARP2 (Several Ankyrin Repeat Protein 2). A PP1 alcança a sua especificidade, não só pela diversidade de proteínas com as quais interage, mas também através de diferentes subunidades catalíticas. No entanto, a maioria dos estudos não aborda directamente a importância das diferentes isoformas da PP1. Neste trabalho relatamos, pela primeira vez, os padrões de expressão de PP1γ2, PP1α e SARP2 no testículo humano, colhido in vivo, por microscopia electrónica. Os nossos resultados demostram que a PP1γ2 é a isoforma mais abundante nos estágios finais de maturação das células germinativas masculinas, enquanto que a PP1α está presente no núcleo dos espermatídeos e durante a formação do acrossoma. A SARP2 é altamente abundante no testículo no qual é expressa apenas na fase pós-meiótica, mais especificamente na fase média/tardia dos espermatídeos haplóides elongados. Dado este padrão de localização de SARP2, propomos que esta possa desempenhar um papel importante na diferenciação e/ou função dos espermatozóides. Em conclusão, a SARP2 e a PP1γ2, proteínas abundantes em testículo e espermatozóides, apresentam a mesma localização no testículo humano. O complexo PP1γ2/SARP2 revela especificidade a nível tecidular (testículo), de estadío de espermatogénese (espermiogénese), a nível celular (espermatídeos elongados) e da localização sub-celular (núcleo), o que torna o complexo um novo alvo para a contracepção masculina ou tratamento de infertilidade masculina.
Protein Phosphatase 1 (PP1) is a major eukaryotic serine/threonine specific phosphatase whose cellular functions depend on the complexes it forms with PP1 Interacting Proteins (PIPs). Given the importance of the testis/sperm-enriched variant, PP1γ2, in spermatogenesis and sperm function it is imperative to identify the roles of the testis/sperm specific PIPs, such as SARP2 (several ankyrin repeat protein 2). PP1 achieves its specificity towards the substrates not only by the diversity of the binding partners but also through different catalytic subunits. Most studies have not directly addressed the significance of the different isoforms. Here, we report, for the first time, the expression patterns of PP1α, PP1γ2 and SARP2 in human testis by immunoelectron microscopy, using tissue previously collected in vivo. Our results show that PP1γ2 is the most abundant isoform in the late stages of germ cell maturation, whereas for the most part, PP1α is found in spermatids nucleus and during the acrosome formation. SARP2 exhibits a tissue, cell type, and spermatogenic stage specificity. SARP2 is more abundant in testis, and within the testis it is expressed only in post-meiotic haploid spermatids, mainly in mid/late stages of elongated spermatids. Given this unique expression pattern of SARP2, we propose that it plays a significant role in sperm differentiation and/or function. In conclusion, SARP2 shows the same localization pattern in human testis as PP1γ2, the PP1 testis/sperm-specific isoform. PP1γ2/SARP2 complex is tissue (testis), spermatogenic stage (spermiogenesis), cell type (elongated spermatids) and subcellular compartment (nucleus) specific. Thus making this complex a putative novel target for male contraception or male infertility treatment.
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Hazra, Rasmani. "Sertoli cell steroid nuclear receptors in testis development and function." Thesis, The University of Sydney, 2014. http://hdl.handle.net/2123/10504.
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Steroid action is vital for the development and function of male gonads. Androgen and the androgen receptor (AR) are critical for the initiation and maintenance of complete spermatogenesis and male fertility. Despite this fundamental role, the precise AR-regulated pathways controlling spermatogenic development have yet to be resolved. A major focus of this research was to use a unique gain-of-function transgenic (Tg) mouse model to determine the temporal role of Sertoli cell-specific AR expression in testicular development. The Sertoli cell-specific rat Abpa promoter directed human Tg AR [Tg Sertoli cell (SC)-specific AR (TgSCAR)] expression, providing strong premature postnatal AR immunolocalized to Sertoli cell nuclei. This novel transgenic model provides unique opportunity to directly differentiate vital AR-regulated genes and regulatory pathways involved in optimal Sertoli cell maturation, and meiotic-postmeiotic germ cell development. The current research has also been investigated the functional role of the Sertoli cell glucocorticoid receptor (GR) in vivo using a Tg Cre-loxP approach to conditionally disrupt GR expression. Sertoli cell GR knock-out (SCGRKO) was shown by absent Sertoli cell-specific GR immunolocalization. This novel SCGRKO model has revealed that Sertoli cell-mediated GR actions support normal testicular function. Sertoli cell GR is required to maintain normal testicular Sertoli/germ cell numbers and circulating gonadotrophin levels, as well as optimal Leydig cell maturation and steroidogenesis, providing new insight into gluocorticoid-mediated impact on male reproduction.
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Almutairi, Mikhlid Hammad. "Identification of novel human cancer-testis-antigen genes in cancers." Thesis, Bangor University, 2014. https://research.bangor.ac.uk/portal/en/theses/identification-of-novel-human-cancertestisantigen-genes-in-cancers(015fda5b-5bd3-42c1-a610-811f0acde19a).html.
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Sousa, Solange Damasceno. "Proteômica do fluido da Rete Testis de carneiros Morada Nova." reponame:Repositório Institucional da UFC, 2015. http://www.repositorio.ufc.br/handle/riufc/19050.
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SOUSA, Solange Damasceno. Proteômica do fluido da Rete Testis de carneiros Morada Nova. 2015. 133 f. : Dissertação (Mestrado) - Universidade Federal do Ceará, Centro de Ciências, Departamento de Zootecnia, Programa de Pós-Graduação em Zootecnia. Fortaleza-CE, 2015.Submitted by Eric Santiago (erichhcl@gmail.com) on 2016-08-10T12:26:45Z No. of bitstreams: 1 2015_dis_sdsousa.pdf: 3355993 bytes, checksum: 3f9c9ea989725756a91c3d6679b0614a (MD5)
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The aim of the study was to identify and characterize the proteins of the rete testis fluid from Morada Nova rams. The testicles, obtained from six slaughtered Morada Nova rams, were immediately dissected. The head of the epididymis was separated to gain access to the efferent ducts. The fluid from the efferent ducts was obtained by testis massage. Thereafter, the fluid was centrifuged to remove cell debris and sperm. Proteins were precipitated with acetone at -20°C and quantified by the Bradford assay. Each sample (400 µg) was focused in strips of 13 cm (pH 4-7) and the second dimension was conducted on SDS-PAGE 15%. The gels were scanned with an ImageScanner II (GE Lifesciences, USA) and analyzed using the PDQuest® version 8.0.1 (Bio-Rad Laboratories, USA). Spots detected after PDQuest analysis of 2-D maps were cut from gels and submitted to trypsin digestion. Proteins were identified using tandem mass spectrometry. Protein information obtained by MASCOT was analyzed using the software tool for searching annotations of proteins (STRAP). Protein-protein interaction networks were obtained from STRING version 9.0 database. In the gels were detected 227 ± 32.1 spots (mean ± SD), where 51% of the proteins were found above 40 kDa, corresponding to 65% of the intensity of all spots detected. Based on gene ontology analysis, the most common biological processes associated with proteins from rete testis fluid were regulation (24.28%) and cellular process (23.27%). Binding (27.42%) and catalytic activity (19.30%) corresponded to the most frequent molecular functions for those proteins. The most intensely expressed proteins were: albumin, clusterin, serotransferrin, immunoglobulin gamma-1 chain and alpha-2-HS-glycoprotein. The rete testis fluid has a large quantity of proteins related to the spermatogenesis. This feature is important in view of the fact that these molecules contribute to the development of the germ cells, as a result of the transport and conversion of substances required to the production of male gametes.
O objetivo do estudo foi identificar e caracterizar as proteínas do fluido da rete testis de carneiros Morada Nova. Os testículos, obtidos de seis carneiros Morada Nova abatidos, foram imediatamente dissecados. A cabeça do epidídimo foi separada para ter acesso aos ductos eferentes. O fluido oriundo dos ductos eferentes foi obtido por massagem do testículo. Posteriormente, o fluido foi centrifugado para remoção dos debris celulares e espermatozoides. As proteínas foram precipitadas com acetona a -20°C e quantificadas pelo método de Bradford. Cada amostra (400 μg) foi focalizada em tiras de 13 cm (pH 4-7) e a segunda dimensão foi realizada em SDS-PAGE a 15%. Os géis obtidos foram escaneados com um ImageScanner II (GE Lifesciences, EUA) e analisados utilizando o aplicativo PDQuest® versão 8.0.1 (Bio-Rad Laboratories, EUA). Os spots detectados após a análise dos mapas 2-D no PDQuest foram cortados dos géis e submetidos a digestão com tripsina. As proteínas foram identificadas por espectrometria de massa em tandem. A informação sobre a proteína adquirida pela busca no MASCOT foi analisada através da utilização do programa para anotações de proteínas (STRAP). Redes de interação proteína-proteína foram obtidas a partir do banco de dados STRING versão 9.0. Nos géis foram detectados em média 227 ± 32,1 spots, onde 51% das proteínas se encontraram acima de 40 kDa representando 65% da intensidade de todos os spots detectados nos géis. Com base na análise da ontologia gênica, os processos biológicos mais comuns associados às proteínas do fluido rete testis foram regulação (24,28%) e processos celulares (23,27%). Ligação (27,42%) e a atividade catalítica (19,30%) corresponderam às funções moleculares mais frequentes. As proteínas mais intensamente expressas foram: albumina, clusterina, serotransferrina, imunoglobulina gama-1 e alfa-2-HS-glicoproteína. A maioria das proteínas identificadas desempenha importantes papéis nos processos de espermatogênese, proteção espermática, motilidade, capacitação e reação acrossômica. O fluido da rete testis possui várias proteínas envolvidas na espermatogênese, o que representa um importante fator, uma vez que estas moléculas contribuem para o desenvolvimento das células germinativas, participando no transporte e conversão de substâncias requeridas para a produção dos gametas masculinos.
44
Euler, Mikael von. "Mechanisms influencing activation and survival of normal and malignant lymphoid cells in the testis /." Stockholm, 2002. http://diss.kib.ki.se/2002/91-7349-419-4.
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Wong, Ching-hang. "Cell-cell interactions and cell junction dynamics in the mammalian testis." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B31993084.
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Eacker, Stephen Matthew. "Effects of androgen receptor mutations on murine testis development and function /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/10279.
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Bensoussan, Karen. "Effects of vitamin E deficiency on the rat testis and epididymis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0007/MQ37092.pdf.
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Baker, Susan Jane. "Regulation of gonadotropin receptors in the testis of the adult ram." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=72092.
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Factors influencing testicular gonadotropin receptors and testis responsiveness to luteinizing hormone (LH) stimulation were studied during the seasonal sexual cycle of the adult ram. The normal variation in testis content of gonadotropin receptors was mapped out in relation to changes in (1) testis size, (2) testis responsiveness to LH stimulation and (3) mean serum concentration of LH, follicle stimulating hormone (FSH), testosterone and prolactin (PRL). The increase in gonadotropin receptor numbers during testicular redevelopment was preceded by elevated serum concentrations of PRL and associated with increases in both the frequency of endogenous pulses of LH and the responsiveness of the testis to LH stimulation. When serum PRL concentrations displayed abnormal variations in relation to photoperiod, the normal patterns of change in testis LH receptors and responsiveness to LH stimulation were altered. To determine if the increase in gonadotropin receptors was due to increased frequency of LH pulses up regulating homologous receptors, rams were injected, in the nonbreeding season with small, frequent doses of LH. Testis responsiveness was greatly enhanced but occurred independently of changes in gonadotropin receptor numbers or their binding affinities. To determine if the seasonal rise in serum PRL concentration was influencing gonadotropin receptors, endogenous secretion was suppressed by treatment with 2-bromo-X-ergocryptine (CB154) prior to and during testicular redevelopment. This resulted in delayed testicular redevelopment and reduced numbers of testicular LH receptors. Reduced numbers of LH receptors was associated with reduced responsiveness of the testis to LH stimulation. These results support the hypothesis that the seasonal increase in serum PRL concentration initiates testicular redevelopment by increasing LH receptors. Increases in LH peak frequency enhance responsiveness but this occurs independently of changes in gonadotropin receptors.
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Chung, Pui-yee Nancy, and 鐘佩儀. "Characterization of tight junctions in the testis: implications in male contraception." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B31224337.
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鍾穗華 and Shui-wah Chung. "Cell-cell interactions in the rat testis: biology and future perspectives." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1999. http://hub.hku.hk/bib/B31238385.
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