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Journal articles on the topic 'Test microbiologico'

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Author: Grafiati

Published: 9 March 2023

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1

Comunian, R., F. Piras, R. Di Salvo, A. Paba, G. Riu, M. Addis, E. P. L. De Santis, and S. Porcu. "Durata di conservazione della carne di suinetto sardo tradizionale e trattata termicamente e confezionata sottovuoto." Archivos de Zootecnia 67, Supplement (January 15, 2018): 173–76. http://dx.doi.org/10.21071/az.v67isupplement.3597.

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Il suinetto da latte arrosto è un piatto tradizionale della Sardegna ampiamente consumato in tutta l’isola e molto apprezzato dai turisti. Purtroppo, a causa della recrudescenza della peste suina africana (PSA) nell’isola, è stata vietata l’esportazione di carni suine fresche e/o prodotti derivati. Alcune precauzioni necessarie per ottenere il permesso di esportare i prodotti a base di carne di maiale sono contenute nel provvedimento attuativo del Programma straordinario di eradicazione della PSA 2015-2017 emanato dal Governo Regionale. Una di queste consiste nel trattamento termico di precottura della carne fino al raggiungimento di una temperatura di 80 °C in tutta la massa. L’esportazione della carne di maiale rappresenta un’importante opportunità economica per gli operatori sardi del settore, fortemente interessati alla dimostrazione dell’efficacia e applicabilità del trattamento termico per garantire la sicurezza e la qualità dei loro prodotti. Lo scopo di questo studio era quello di valutare, mensilmente, da 0 a 150 giorni, la shelf-life dei campioni di carne trattati termicamente e conservati sottovuoto a 4 °C. Pertanto, cinque mezzene di suinetto, per punto di campionamento, sono state analizzate da un punto di vista microbiologico (conta di batteri patogeni e dannosi) e chimico (perossidazione lipidica), prima del completamento della cottura. Dopo 40 min in forno a 210 °C, è stato eseguito test di accettabilità sensoriale, per valutare il gradimento dei consumatori. I risultati ottenuti consentono di concludere che, la contaminazione microbica post-processo non ha determinato un decadimento della qualità e dell’accettabilità sensoriale del prodotto, fino a 150 giorni di shelf-life. I risultati evidenziano la necessità di prendere in considerazione l’applicazione di un trattamento post-letale per ridurre la contaminazione microbica.

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Giorgio, Antonella, Laura Miele, Salvatore Bonis, Irene Conforti, Luigi Palmiero, Marco Guida, Giovanni Libralato, and Francesco Aliberti. "Microbiological Stability of Cosmetics by using Challenge Test Procedure." Journal of Pure and Applied Microbiology 12, no. 1 (March 30, 2018): 23–28. http://dx.doi.org/10.22207/jpam.12.1.04.

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3

Tidswell, Edward C., and Tim Sandle. "Microbiological Test Data—Assuring Data Integrity." PDA Journal of Pharmaceutical Science and Technology 72, no. 1 (October 12, 2017): 2–14. http://dx.doi.org/10.5731/pdajpst.2017.008151.

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4

Allcock, Richard J. N., Amy V. Jennison, and David Warrilow. "Towards a Universal Molecular Microbiological Test." Journal of Clinical Microbiology 55, no. 11 (August 23, 2017): 3175–82. http://dx.doi.org/10.1128/jcm.01155-17.

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ABSTRACTThe standard paradigm for microbiological testing is dependent on the presentation of a patient to a clinician. Tests are then requested based on differential diagnoses using the patient's symptoms as a guide. The era of high-throughput genomic methods has the potential to replace this model for the first time with what could be referred to as “hypothesis-free testing.” This approach utilizes one of a variety of methodologies to obtain a sequence from potentially any nucleic acid in a clinical sample, without prior knowledge of its content. We discuss the advantages of such an approach and the challenges in making this a reality.

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5

IJzerman-Boon, Pieta C., and Edwin R. van den Heuvel. "Validation of qualitative microbiological test methods." Pharmaceutical Statistics 14, no. 2 (November 21, 2014): 120–28. http://dx.doi.org/10.1002/pst.1663.

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6

Norman, Savanah, Alexandra Burton, Christy Mumphrey, Peter Joslyn, and Gregory Cook. "1140. Impact of a Rapid Molecular Bloodstream Diagnostic Test on Optimal Antibiotic Use in Infants." Open Forum Infectious Diseases 8, Supplement_1 (November 1, 2021): S661. http://dx.doi.org/10.1093/ofid/ofab466.1333.

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Abstract Background Rapid molecular bloodstream diagnostics have been shown to decrease time-to-optimal antibiotic therapy in adult and pediatric patients. The purpose of the study was to compare the time-to-optimal antimicrobial therapy both pre-and post- implementation of rapid diagnostic testing in infants. Methods This was a single-center quasi-experimental study conducted from December 2018 to December 2020 at Children’s Hospital New Orleans. A rapid, multiplex polymerase chain reaction bloodstream diagnostic was implemented in January 2019. Antimicrobial Stewardship performed a daily review of all antimicrobials during both periods and made recommendations when necessary. The primary outcome was the difference in time-to-optimal therapy. Secondary outcomes included time-to-effective therapy, 30-day all-cause mortality rate, 30-day recurrent bacteremia rate, and time-to-microbiologic clearance. Patients were excluded if they had an unrelated concomitant infection, withdrawal of care before the result, bacteria not identified by the panel, or were over 6 months of age. Results Thirty-five and forty-three patients met inclusion criteria pre-and post-implementation. The median post-natal age was 2 months and median PRISM score was 12 in both groups. Median time-to-optimal therapy was 53.1 hours in the pre-intervention and 24.4 hours in the post-intervention group (-28.7 hours, P = 0.03). Median time-to-effective therapy was 0 and 1.4 hours, respectively (+1.4 hours, P = 0.02). There was no significant difference in 30-day all-cause mortality (3 vs. 4 patients, P = 0.62), 30-day recurrent bacteremia (0 vs. 2 patients, P = 0.2), or microbiologic clearance (37.3 vs. 26.2 hours, P = 0.09). Conclusion Implementation of a rapid, multiplex bloodstream diagnostic lead to a significant decrease in time-to-optimal antibiotic therapy in infants when compared to standard microbiological techniques. Disclosures All Authors: No reported disclosures

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7

Kapronezai, J., P. Melville, and N. R. Benites. "ANÁLISE MICROBIOLÓGICA, TESTE DE TAMIS E CALIFORNIA MASTITIS TEST REALIZADOS EM AMOSTRAS DE LEITE DE FÊMEAS BUBALINAS PERTENCENTES A REBANHOS DO ESTADO DE SÃO PAULO." Arquivos do Instituto Biológico 72, no. 2 (April 2005): 181–85. http://dx.doi.org/10.1590/1808-1657v72p1812005.

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RESUMO O presente trabalho teve como objetivo a análise dos testes de tamis, CMT e do exame microbiológico de amostras de leite de fêmeas bubalinas. Os quartos mamários foram submetidos ao teste de tamis e CMT, sendo colhidas 262 amostras de leite de fêmeas bubalinas primíparas e pluríparas para análise microbiológica. No teste do tamis, 99,6% das amostras apresentaram resultado negativo. No CMT, observamos resultado negativo em 88,2% das amostras; traços (8%), positivo 1+ (1,5%); positivo 2+ (1,15%); positivo 3+ (1,15%). A análise microbiológica apresentou amostras sem crescimento de microrganismos (75,6%); com isolamento de Staphylococcus spp. (11,8%), Corynebacterium spp. (7,3%), Streptococcus spp. (3,1%) e com crescimento de microrganismos em associações (1,2%). Concluiu-se que nos rebanhos bubalinos estudados, os índices de mastite clínica e subclínica são muito baixos. A análise dos resultados obtidos no CMT e no exame microbiológico nos leva a inferir que a freqüência de animais portadores nos rebanhos estudados é elevada ou que o teste de CMT não pode ser considerado um bom teste de triagem para mastite em bubalinos, o que evidencia a importância da adoção de medidas preventivas a fim de assegurar a qualidade e inocuidade do produto e de seus derivados.

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Augustin, Jean-Christophe, and Vincent Carlier. "French Laboratory Proficiency Testing Program for Food Microbiology." Journal of AOAC INTERNATIONAL 85, no. 4 (July 1, 2002): 952–59. http://dx.doi.org/10.1093/jaoac/85.4.952.

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Abstract The proficiency testing program in food microbiology (Réseau d' Analyses et d'Echanges en Microbiologie des Aliments; RAEMA), created in 1988, currently includes 440 participating laboratories. The program establishes proficiency in detection of Salmonella and Listeria monocytogenes, as well as quantitation of aerobic microorganisms, Enterobacteriaceae, coliforms, Escherichia coli, Clostridium perfringens, coagulase-positive Staphylococcus, and Listeria monocytogenes. Twice a year, 5 test samples are sent to participants to assess their precision and trueness for enumeration and detection of microorganisms. Results show an increasing involvement of food microbiology laboratories in quality assurance programs and use of standard and validated analytical methods. However, the percentage of laboratories obtaining questionable and unsatisfactory microbiological results remains relatively onstant.

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9

Mori, Yoko. "Reliability of the Microbiological Test Results in Foods." Japanese Journal of Food Microbiology 33, no. 3 (2016): 127–33. http://dx.doi.org/10.5803/jsfm.33.127.

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10

SEMENZATO, A., C. A. BENASSI, G. ROSSI, A. BETTERO, M. LUCCHIARI, and R. CERINI. "Dowicil 200 Stability test: chemical and microbiological studies." International Journal of Cosmetic Science 12, no. 6 (December 1990): 265–72. http://dx.doi.org/10.1111/j.1467-2494.1990.tb00541.x.

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11

方, 燕玲. "Research on Microbiological Limit Test of ZuoGui Pills." Pharmacy Information 09, no. 03 (2020): 126–30. http://dx.doi.org/10.12677/pi.2020.93018.

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12

Lucci, Arturo, Carlo P. Campobasso, Antonello Cirnelli, and Giulio Lorenzini. "A promising microbiological test for the diagnosis of drowning." Forensic Science International 182, no. 1-3 (November 2008): 20–26. http://dx.doi.org/10.1016/j.forsciint.2008.09.004.

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13

Noblett, Tracey. "The effects of transport on microbiological proficiency test samples." Accreditation and Quality Assurance 20, no. 4 (May 23, 2015): 329–33. http://dx.doi.org/10.1007/s00769-015-1138-z.

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14

Meagher, A. K., J. A. Passarell, B. B. Cirincione, S. A. Van Wart, K. Liolios, T. Babinchak, E. J. Ellis-Grosse, and P. G. Ambrose. "Exposure-Response Analyses of Tigecycline Efficacy in Patients with Complicated Skin and Skin-Structure Infections." Antimicrobial Agents and Chemotherapy 51, no. 6 (March 12, 2007): 1939–45. http://dx.doi.org/10.1128/aac.01084-06.

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ABSTRACT Exposure-response analyses were performed for the microbiological and clinical efficacy of tigecycline in the treatment of complicated skin and skin-structure infections, where Staphylococcus aureus and streptococci are the predominant pathogens. A prospective method was developed to create homogeneous patient populations for PK-PD analyses. Evaluable patients from three clinical trials were pooled for analysis. Patients received a tigecycline 100-mg loading dose/50 mg every 12 h or a 50-mg loading dose/25 mg every 12 h. At the test-of-cure visit, microbiologic and clinical responses were evaluated. Patients were prospectively evaluated and classified into cohorts based on baseline pathogens: S. aureus only (cohort 1), monomicrobial S. aureus or streptococci (cohort 2), two gram-positive pathogens (cohort 3), polymicrobial (cohort 4), or other monomicrobial infections (cohort 5). A prospective procedure for combining cohorts was used to increase the sample size. Logistic regression evaluated steady-state 24-h area under the concentration-time curve (AUC24)/MIC ratio as a predictor of response, and classification and regression tree (CART) analyses were utilized to determine AUC/MIC breakpoints. Analysis began with pooled cohorts 2 and 3, the focus of these analyses, and included 35 patients with 40 S. aureus and/or streptococcal pathogens. CART analyses identified a significant AUC/MIC breakpoint of 17.9 (P = 0.0001 for microbiological response and P = 0.0376 for clinical response). The continuous AUC/MIC ratio was predictive of microbiological response based on sample size (P = 0.0563). Analysis of all pathogens combined decreased the ability to detect exposure-response relationships. The prospective approach of creating homogeneous populations based on S. aureus and streptococci pathogens was critical for identifying exposure-response relationships.

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15

Aesif, Scott W., David M. Parenti, Linda Lesky, and John F. Keiser. "A Cost-Effective Interdisciplinary Approach to Microbiologic Send-Out Test Use." Archives of Pathology & Laboratory Medicine 139, no. 2 (April 23, 2014): 194–98. http://dx.doi.org/10.5858/arpa.2013-0693-oa.

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Context Use of reference laboratories for selected laboratory testing (send-out tests) represents a significant source of laboratory costs. As the use of more complex molecular analyses becomes common in the United States, strategies to reduce costs in the clinical laboratory must evolve in order to provide high-value, cost-effective medicine. Objective To report a strategy that employs clinical pathology house staff and key hospital clinicians in the effective use of microbiologic send-out testing. Design The George Washington University Hospital is a 370-bed academic hospital in Washington, DC. In 2012 all requisitions for microbiologic send-out tests were screened by the clinical pathology house staff prior to final dispensation. Tests with questionable utility were brought to the attention of ordering clinicians through the use of interdisciplinary rounds and direct face-to-face consultation. Results Screening resulted in a cancellation rate of 38% of send-out tests, with proportional cost savings. Nucleic acid tests represented most of the tests screened and the largest percentage of cost saved through screening. Following consultation, requested send-out tests were most often canceled because of a lack of clinical indication. Conclusions Direct face-to-face consultation with ordering physicians is an effective, interdisciplinary approach to managing the use of send-out testing in the microbiology laboratory.

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Butko, M. P., and D. A. Onishchenko. "APPLICATION OF THE TEST SYSTEM PREMI®TEST FOR THE DETECTION OF ANTIBACTERIAL SUBSTANCES IN FISH AND FISH PRODUCTS." Problems of Veterinary Sanitation, Hygiene and Ecology 1, no. 4 (2018): 6–14. http://dx.doi.org/10.36871/vet.san.hyg.ecol.201804001.

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The article covers the issues of adaptation and application of the Premi®Test test system for accelerated detection of residual amounts of antibacterial substances in fish and fish products. Experiments were conducted to determine the detection limit of a number of antibacterial substances; at the second stage, a series of experiments were conducted to assess the influence of various factors of the analysis (temperature of sample, amount of material under study, sample storage time, sample grinding method, etc.) on the results of studies by the Premi®Test test system. The obtained experimental data and production tests allowed us to confirm and offer an effective microbiological method based on Premi®Test for the detection of antibacterial substances in fish and fish products.

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Arismendi-Morillo, Gabriel, Ileana Hernández, Edgardo Mengual, Alisbeth Fuenmayor, Gisela Romero, and Maribel Lizarzábal. "Comparison of three methods based on endoscopic gastric biopsies for diagnosis of Helicobacter pylori active infection in a clinical setting." Arquivos de Gastroenterologia 48, no. 3 (September 2011): 190–94. http://dx.doi.org/10.1590/s0004-28032011000300007.

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CONTEXT: The correct diagnosis and effective treatment of Helicobacter pylori gastric infection are essential in controlling this infection. OBJECTIVE: To compare the diagnostic value of three tests based in endoscopic gastric biopsies histopathological evaluation with hematoxylin-eosin (H-E) staining, urease rapid test and microbiological culture for detecting Helicobacter pylori active infection, in order to make recommendations for daily clinical practice. METHODS: Gastric biopsies from 115 adult patients (85 female/30 male) were obtained by upper gastrointestinal endoscopy and studied by histopathological evaluation with H-E (antrum-corpus), urease test in 2 hours (antrum) and microbiological culture (antrum). RESULTS: Helicobacter pylori active infection was diagnosed in 67% of patients. Helicobacter pylori active infection was detected by histopathological evaluation with H-E, urease test and microbiological culture in 87%, 79% and 70% of the positive cases, respectively. There were significant differences when histopathological evaluation with H-E and urease test rapid test when compared with microbiological test (P<0.01). There was no significant difference between histopathological evaluation with H-E and urease test (P = 0.7). The kappa index of agreement for histopathological evaluation with H-E/urease test was 0.56, histopathological evaluation with H-E/microbiological culture 0.6, and urease test/microbiological culture 0.64. CONCLUSIONS: In a hospital setting like the one studied, histopathological evaluation with H-E and urease test are the most recommended tests for diagnosis of Helicobacter pylori active infection based in endoscopic biopsies. If pathological information of gastric lesions will be required, histopathological evaluation with H-E is essential. Urease test is mandatory if a prompt diagnosis is necessary. Microbiological culture can be used in cases of persistent or complicated infection, which may require studies on Helicobacter virulence or antimicrobial susceptibility. Selected cases might demand a combination of several tests. The three tests exhibit a good concordance level for Helicobacter pylori active infection diagnosis.

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Tokyol, Çiğdem, Orhan Cem Aktepe, Arif Serhan Cevrioğlu, Mustafa Altındiş, and Fatma Hüsniye Dilek. "Bacterial vaginosis: comparison of Pap smear and microbiological test results." Modern Pathology 17, no. 7 (April 9, 2004): 857–60. http://dx.doi.org/10.1038/modpathol.3800132.

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Lucci, A., and A. Cirnelli. "A microbiological test for the diagnosis of death by drowning." Forensic Science International 168, no. 1 (May 2007): 34–36. http://dx.doi.org/10.1016/j.forsciint.2006.06.050.

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20

Loborec, Steven M., Jose A. Bazan, Nicole V. Brown, Mary Beth Shirk, and Trisha A. Jordan. "Privileging pharmacists improves time to patient notification in the microbiological test review process for patients discharged from the emergency department." American Journal of Health-System Pharmacy 77, Supplement_1 (January 21, 2020): S19—S24. http://dx.doi.org/10.1093/ajhp/zxz335.

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Abstract Purpose Results of a study evaluating the impact of privileging pharmacists to manage microbiologic test results for patients discharged from the emergency department (ED) are reported. Methods This was a single-center, retrospective pre-post study that was conducted at an urban academic medical center. Patients discharged from the ED with a subsequent positive microbiologic test result before and after privileging of an ED specialty practice pharmacist (ED-SPP) to manage the results independently were screened for inclusion. Time to patient notification of a required change in antimicrobial therapy was compared between groups. Numbers of erroneous interventions before and after pharmacist privileging were compared to assess the safety of implementation. Results One hundred seventy-eight positive microbiologic test results (n = 92 pre- and n = 86 postimplementation) were included. The median time to patient notification in the pre-implementation group was 23.6 hours (range, 12.4-93 hours) and in the postimplementation group was 14.9 hours (range, 2.5-27.9 hours; P = 0.0023). As determined by the board-certified infectious disease physician, 1.1% of reviewed microbiologic test results (1 of 92) was erroneous prior to implementation of pharmacist privileging compared with 2.3% (2 of 86) after implementation (P = 0.6105). Conclusion Privileging ED-SPPs to assess microbiologic test results improved the time to patient notification with no statistical difference in the number of erroneous interventions between groups. These findings demonstrate the benefit of clinical privileging and provide support for expansion of this role to other ED-SPPs.

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Petrovic, Jelena, Brankica Kartalovic, Radomir Ratajac, Jasna Prodanov-Radulovic, Igor Stojanov, Marina Zekic, and Srdjan Stefanovic. "Detection of enrofloxacine residues by microbiological screening method." Biotehnologija u stocarstvu 35, no. 1 (2019): 49–59. http://dx.doi.org/10.2298/bah1901049p.

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The usage of microbiological screening tests is widespread in control of presence of antimicrobial drug residues in meat samples. Screening tests must be capable to detect antimicrobial drug residue of interest and detection limits must comply with MRL (Maximum Residue Limit). The aim of this study was to examine the performance of a microbiological screening test with E. coli as test microorganism: capability of detecting enrofloxacina and it?s main metabolite ciprofloxacine at MRL levels in both fortified and incurred chicken tissue samples. Detection limits of microbiological screening test with E. coli was 50 ng/g for enrofloxacin and 25 ng/g for ciprofloxacin. Screening test had positive results in all samples of fortified and incurred meat with residue concentrations above MRL level. The results of this examinations shows that microbiological screening test with E. coli, as simple and cost effective test, is capable to detect enrofloxacine and it?s metabolite ciprofloxacine in treated poultry at MRL level ie test is capable to detect unsafe poultry meat.

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Van Houte, J. "Microbiological Predictors of Caries Risk." Advances in Dental Research 7, no. 2 (August 1993): 87–96. http://dx.doi.org/10.1177/08959374930070022001.

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The prediction of caries risk has been of longstanding interest. Generally, few of the tests involving oral bacteria or their products have become accepted. Presently, the main focus is on counts of lactobacilli (L) and mutans streptococci (MS). Due to their positive numerical association with human caries and the linkage of this association to carbohydrate consumption, counts of L and MS may, potentially, serve not only as a caries risk predictor but also as an indicator of carbohydrate consumption, another caries-risk factor. The value of counts of L and MS as caries-risk predictors has been evaluated by means of studies providing data on test sensitivity, specificity, and predictive values. These and other studies indicate that their use for the prediction of caries risk of individuals is not possible but is more promising for that of the caries risk of groups (e.g., identification of high-caries-risk subjects); further, the prediction of low caries risk may be more reliable than that of high caries risk. The influence of test variables on the test results has been discussed. These include the level of caries increment, subject age, methods of caries evaluation, use of saliva or dental plaque as test sample, sampling frequency, type of bacterial growth medium, and the use of simplified methods rather than conventional laboratory procedures for microbial enumeration. An approach to optimize the use of microbiological caries-risk predictors in different populations as well as their use in conjunction with other caries-risk predictors has been discussed. The latter include the incipient caries lesion or past caries experience and salivary buffering capacity and flow rate. Due to the multifactorial nature of caries etiology, it is expected that multivariate approaches rather than the use of single parameters may improve caries risk prediction for individuals as well as groups of subjects.

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Moraes, Aline Viana, Eduardo Santos Bretas, Camilo Dias Seabra Pereira, Fernando Sanzi Cortez, Augusto Cesar, and Aldo Ramos Santos. "Avaliação da qualidade ambiental do rio Itaguaré, Bertioga-SP, com base em testes de toxicidade e indicadores microbiológicos de balneabilidade." O Mundo da Saúde 35, no. 1 (March 30, 2011): 55–63. http://dx.doi.org/10.15343/0104-7809.20113515563.

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YAMAMOTO, Hiroshi, Hiroshi SATO, Ken-ichi YAGAMI, Jiro ARIKAWA, Masato FURUYA, Tsutomu KUROSAWA, Kazuaki MANNEN, et al. "Microbiological Contamination in Genetically Modified Animals and Proposals for a Microbiological Test Standard for National Universities in Japan." Experimental Animals 50, no. 5 (2001): 397–407. http://dx.doi.org/10.1538/expanim.50.397.

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Kim, Henry S., and Gerald Angyal. "Comparison of Liquid Chromatographic Method to AOAC Microbiological Method for Determination of L-Tryptophan in Tablets and Capsules." Journal of AOAC INTERNATIONAL 76, no. 2 (March 1, 1993): 414–17. http://dx.doi.org/10.1093/jaoac/76.2.414.

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Abstract A reversed-phase liquid chromatographic (LC) method coupled with precolumn derivatization of L-tryptophan with phenylisothiocyanate was compared to the AOAC microbiological method for determining L-tryptophan in tablets and capsules. For the microbiological method, the concentrations of L-tryptophan were 4-8% lower in autoclaved test samples (hot method) than in test samples that were not autoclaved (cold method). When L-tryptophan values obtained by the LC method were compared to those obtained by the cold microbiological method, no significant differences were observed (P > 0.05). The mean relative standard deviations were 2.9% for the LC method and 1.6% for the cold microbiological method. The mean recoveries of standard L-tryptophan added before analysis were 99% for the LC method and 101 % for the cold microbiological method. These results demonstrate that both methods are reliable for determining free L-tryptophan contained in tablets and capsules. However, the LC method has the advantages of using a smaller test portion and having a shorter analysis time.

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Hama, Koji, Tohru Hashida, and Kazusaburo Kataoka. "Microbiological Challenge Test of Contamination Caused by Using the PhaSeal System." Iryo Yakugaku (Japanese Journal of Pharmaceutical Health Care and Sciences) 39, no. 3 (2013): 148–55. http://dx.doi.org/10.5649/jjphcs.39.148.

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KÜMMERLIN, R. "Technical note: Resazurin test for microbiological control of deep-frozen shrimps." International Journal of Food Science & Technology 17, no. 4 (June 28, 2007): 513–15. http://dx.doi.org/10.1111/j.1365-2621.1982.tb00207.x.

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Swenson, Jana M., Robert Skov, and Jean B. Patel. "The cefoxitin disk test – what a clinical microbiologist needs to know." Clinical Microbiology Newsletter 29, no. 5 (March 2007): 33–40. http://dx.doi.org/10.1016/j.clinmicnews.2007.02.004.

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Hong, DY, SO Park, KR Lee, KJ Baek, HW Moon, SB Han, and DH Shin. "Bacterial Contamination of Computer and Hand Hygiene Compliance in the Emergency Department." Hong Kong Journal of Emergency Medicine 19, no. 6 (November 2012): 387–93. http://dx.doi.org/10.1177/102490791201900603.

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Introduction The aim of this study was to determine the degree and nature of bacterial contamination of computer equipment in three Korean emergency departments (ED). Methods Hand hygiene practices of ED doctors and nurses were observed before contact with computer equipment. Microbiological swab samples were obtained from 112 multiple-user computer keyboards and electronic mice in the ED of three teaching hospitals. Isolated organisms were identified by a clinical microbiologist using Gram stain, colony morphology, and susceptibility test. Results Of the 112 samples, 103 (92.0%) showed growth of organisms on culture. Thirty-eight (33.9%) pieces of computer equipment yielded multiple bacterial species. Coagulase-negative Staphylococcus was the most common microorganism isolated (85.7%). Methicillin-resistant Staphylococcus aureus was obtained from two keyboards in two hospitals (1.8%). Hand hygiene compliance was observed on 29.9% occasions. Hand hygiene compliance after patient contact (38.0%) was higher than after other environmental contact (20.7%). Conclusions Multiple user computer equipment in the ED may serve as reservoirs for nosocomial infection. Hand hygiene should be performed before and after using all ED equipment, including computer equipment.

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Dillon, William, Tommy J. Parraga Acosta, Andrew J. Failla, Julio Corrales, Ramesh Mayur, and George J. Alangaden. "593. Utility of Microbiologic Testing Obtained via Bronchoalveolar Lavage on Asymptomatic Lung Transplant Recipients: A Quality Improvement Study." Open Forum Infectious Diseases 8, Supplement_1 (November 1, 2021): S400. http://dx.doi.org/10.1093/ofid/ofab466.791.

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Abstract Background The utility of surveillance bronchoscopy (SB) in asymptomatic lung transplant recipients (LTR) is controversial. Guidelines regarding the timing of SB and diagnostic testing varies across centers. Studies evaluating the role of microbiologic testing are lacking. Our transplant institute currently performs SB at week 1, and months 1, 3, 6, 9, 12, and 24 post-transplant. We evaluated if routine microbiologic testing obtained during SB impacted clinical management. Methods This observational cohort study was performed at Henry Ford Hospital, Detroit, MI and included all LTR done from August 2014 to August 2019. Clinical and laboratory data was abstracted from the electronic medical record Pre/post-SB. Bronchoscopies performed for new or worsening respiratory symptoms, decline in forced expiratory volume at one second ≥10%, new radiographic abnormalities and follow up bronchoscopies to assess stents or recent acute rejection were excluded. Microbiologic tests assessed are shown in Table 2. Management change was defined as reduction in immunosuppression or prescription of antimicrobials. Rate of change in clinical management based on microbiologic test positivity was the primary outcome. Data were analyzed with descriptive statistics. Results 449 SB in 107 LTR were evaluated. Median age was 63 years, 68% were male. The average number of SB performed per patient was 4.2 (Table 1). The most common microbiologic tests performed were bacterial (435), mycobacterial (427), and fungal including Pneumocystis jirovecii (1022) (Table 2). The rate of test positivity and resultant change in management are shown in Table 3. The rate of test positivity was highest for bacterial (54%), fungal (27%) and viral tests (6%) with management changes in 12%, 2%, and 3% respectively. Table 1. Patient Demographics Table 2. Rate of Microbiologic Testing per Surveillance Bronchoscopy Table 3. Rate of Microbiologic Positivity and Management Change per Surveillance Bronchoscopy Conclusion This is the largest study to specifically evaluate the role of routine microbiologic tests during SB in LTR. Bacterial cultures may be appropriate due to higher rates of management changes. However, routine fungal, AFB, and viral studies are unnecessary due to low true positivity, and consequent low rate of management changes. This represents an important opportunity for diagnostic and antimicrobial stewardship. Disclosures All Authors: No reported disclosures

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Guglielmo, Mona, Lucia Chen, Sitaram Vangala, Michelle Lim, Arunima Bera, Tanaya Deshmukh, Paul Krogstad, and Anil Sapru. "353: RELATIONSHIP OF A POSITIVE MICROBIOLOGIC TEST TO CLINICAL OUTCOMES IN PEDIATRIC ARDS." Critical Care Medicine 48, no. 1 (January 2020): 158. http://dx.doi.org/10.1097/01.ccm.0000619764.36834.f6.

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Hutapea, Henny, Yulia Shara Sembiring, and Nathanael Setna. "Effect of Acid-Based Level on Storage Acrylic Emulsion Paint." Indonesian Journal of Chemical Studies 1, no. 1 (June 11, 2022): 13–15. http://dx.doi.org/10.55749/ijcs.v1i1.4.

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In the present study, a novel methodology was developed for the assessment of acid-based effect levels on the storage acrylic emulsion paints., It was found that the acrylic emulsion paint was clumping and smelled bad on the organoleptic test. Most bacterial growth on the microbiological test. This occurred in the acrylic emulsion paint sample which had a pH level of 7. Microbiological checking found little bacterial growth in the paint which had a level of pH 9, which is relatively safe in the storage process for 30 days and has good results in organoleptic and microbiological tests.

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Cosgarea, Raluca, Amelie Bäumer, Bernadette Pretzl, Sven Zehaczek, and Ti-Sun Kim. "Comparison of two different microbiological test kits for detection of periodontal pathogens." Acta Odontologica Scandinavica 68, no. 2 (March 2010): 115–21. http://dx.doi.org/10.3109/00016350903514848.

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Dey, B. P., Alice Thaler, and Frank Gwozdz. "Analysis of Microbiological Screen Test Data for Antimicrobial Residues in Food Animals." Journal of Environmental Science and Health, Part B 38, no. 3 (April 2003): 391–404. http://dx.doi.org/10.1081/pfc-120019904.

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Patel, Vimal. "Performance Comparison of Rapid Petrifilm™ Test Methods vs. Conventional Test Methods for Microbiological Testing of Dietary Supplements." Current Developments in Nutrition 4, Supplement_2 (May 29, 2020): 1832. http://dx.doi.org/10.1093/cdn/nzaa067_059.

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Abstract Objectives The aim of this study is to show suitability of 3M™ Petrifilm™ across various types of common multi ingredient finished dietary supplement products. Methods In present investigation, parallel analysis and suitability was conducted using gold standard methods prescribed under U.S. Pharmacopeia general chapter dietary supplement ⟨2021⟩/⟨2022⟩, US FDA's Bacteriological Analytical Manual (BAM), and AOAC using 3M™ Petrifilm™. Five commonly used tests in food and dietary supplement quality testing were deployed in this study, namely: Total Aerobic Microbial Count (TAMC), Total Yeast and Mold Count (TYMC), Escherichia coli, Staphylococcus aureus, and Coliforms. Dietary supplements selected for this investigation are from most common types of dietary supplements categories sold in US market i.e., multivitamin and mineral, protein, multi mineral, Prenatal, Vitamin D, and Omega 3 Fish oil. Three individual lots of each of the five products were tested to increase robustness of data. Acceptance criteria was set same as USP ⟨2021⟩/⟨2022⟩ requirements, that is to demonstrate a greater than 70% recovery in comparison to a control. Results AOAC using 3M™ Petrifilm™ results show recovery of >70% compared to control for all five test Total Aerobic Microbial Count (TAMC), Total Yeast and Mold Count (TYMC), Escherichia coli, Staphylococcus aureus, and Coliforms: Multivitamin and mineral product recovery ranges were between 79%–111%, Protein product recovery ranges were between 94%–104%, Multi mineral product recovery ranges were between 94%–107%, Prenatal product recovery ranges were between 74%–117%, Vitamin D product recovery ranges were between 93%–123%, and Omega 3 fish oil product recovery ranges were between 83%–101%. Conclusions We found that AOAC based 3M™ Petrifilm™ methods are suitable for selected major categories of dietary supplement finished products. All results from AOAC using 3M™ Petrifilm™ microbiological testing achieved >70% bioburden recovery in comparison to a control. We also found that AOAC using 3M™ Petrifilm™ provides consistent and comparable results to USP ⟨2021⟩/⟨2022⟩/FDA's BAM results for the same samples as well. Funding Sources Reliance Vitamin.

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Raghavendra Babu, N. "MICROBIOLOGICAL ANALYTICAL METHOD FOR DETERMINATION OF SERTACONAZOLE NITRATE IN PHARMACEUTICAL FORMULATIONS." YMER Digital 21, no. 07 (July 28, 2022): 1104–15. http://dx.doi.org/10.37896/ymer21.07/91.

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Sertaconazole nitrate is a third generation synthetic broad-spectrum, benzothiophene imidazole antifungal agent. A new microbiological method was developed for analysis of sertaconazole tablets using candida Albicans as test microorganism. The diffusion assay method was optimized by using different media, organisms and conditions. Prospective validation of the method showed adequate linearity (r = 0.9964), precision (% RSD<2%) and accuracy (mean recovery = 98.26%). Highperformance liquid chromatography was chosen as a comparison method for voriconazole determination. Results of both the microbiological and HPLC methods were compared with student ttest and the contents of voriconazole determined by both methods, showed a strong correlation. Highperformance liquid chromatography was chosen as a comparison method. Results of both the microbiological and HPLC methods were compared with student t-test and the contents of sertaconazole determined by both methods showed a strong correlation. The microbiological analytical method which was developed gives true indication of biological activity and can be used for routine quality control analysis of sertaconazole nitrate in dosage forms. Key words: Sertaconazole nitrate, t-test, Candida albicans, Diffusion method, HPLC, Method validation.

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Lodes, Uwe, Beate Bohmeier, Frank Meyer, Brigitte Koenig, and Hans Lippert. "W1043 Microbiologic Diagnostic with the Novel Lightcycler Septifast® Test During the Early Phase of Surgical Sepsis Mainly Caused By Peritonitis Is Quicker and More Sensitive Than Conventional Microbiological Culture." Gastroenterology 134, no. 4 (April 2008): A—621. http://dx.doi.org/10.1016/s0016-5085(08)62901-7.

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Vukovic, V., M. Vicentijevic, and N. Plavsa. "Stability test for mastitis reagent ad us. vet." Biotehnologija u stocarstvu 29, no. 2 (2013): 399–404. http://dx.doi.org/10.2298/bah1302399v.

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In the stability test of the mastitis reagent ad us.vet., as the finished product, three production series were tested in quantities of 500 ml of the sample, under appropriate storage conditions. For the testing, the appropriate uniformity of temperature and relative humidity was provided. Also, the procedure of the stability test was determined, which included the initial state, then every three months until the end of the first trial and a final testing at the end of shelf life (0, 3, 6, 9, 12 and 18 months). Of the tested parameters the following were included: appearance, pH value of the solution, dry residue (in %) and microbiological purity.

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Rehman, Sijad-Ur, Bilal Noor, Muhammad Ishaq, Kaleem Ullah, Gule Lala, and Romana Bibi. "Infection of the Urinary Tract and its Prevalence Among Children Presenting with Malnutrition." Pakistan Journal of Medical and Health Sciences 16, no. 4 (April 26, 2022): 857–60. http://dx.doi.org/10.53350/pjmhs22164857.

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Background: UTIs are a common cause of acute illness in newborns and young children, affecting 8% of girls and 2% of boys by age seven, with a recurrence rate of 10% to 30%. A UTI is a serious condition that can develop into sepsis and other life-threatening consequences in children Objective: To assess the frequency of urinary tract infections in malnourished children. Material and Methods: The study included 241 patients from the Pediatric Department of Children Gajju khan medical collage Swabi. Pakistan. July 7, 2019 – January 7, 2020, All children had urine samples taken by urine bags and catheterization and forwarded to the hospital laboratory to test for UTIs. The same consultant microbiologist with at least five years of expertise supervised all laboratory studies. Results: As per frequencies and percentages for UTIs, 19 (7.88%) patients had UTIs. Conclusion: Given the high frequency of UTIs in children with severe malnutrition, additional research utilizing standardized microbiological approaches is essential. To support the treatment recommendations for UTIs in these children, using urine dipsticks and microscopy in conjunction with urine culture is critical. Keywords: Protein Energy Malnutrition, Bacterial Infection, Urinary Tract Infections.

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Jericó, Marli de Carvalho, Valéria Castilho, and Márcia Galan Perroca. "Training program on microbiological test collection material methods at a teaching hospital: investment and result assessment." Revista Latino-Americana de Enfermagem 14, no. 5 (October 2006): 749–54. http://dx.doi.org/10.1590/s0104-11692006000500017.

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This study aimed at evaluating the results, direct costs and investment of a training program on microbiological test material collection at a teaching hospital. Test collections that did not follow the established criteria (failure) were considered as the result measure. Variable and absorption costing were used to calculate direct costs and investments, respectively. Of the 11,893 collected materials, failures were evidenced in 59 (0.5%). Direct cost corresponded to R$ 154.10 and R$ 2,431.29 was invested in training. These findings revealed that the evidenced number of anomalies (failures) represented a low percentage in relation to the total collected material for microbiological exams. Therefore, this should not be considered a critical point that justifies the continuity of the training and, consequently, the investment.

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Saldaña, Napoleón González, Mercedes Macías Parra, Hugo Juárez Olguín, José Iván Castillo Bejarano, Monica Punzo Soto, and Francisca Trujillo Jiménez. "Tuberculosis in Children in a Pediatric Hospital in Mexico." American Journal of Tropical Medicine and Hygiene 106, no. 1 (January 5, 2022): 75–79. http://dx.doi.org/10.4269/ajtmh.20-1482.

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ABSTRACT. Tuberculosis (TB) remains a global problem and a diagnostic challenge, especially in pediatrics. The aim of this study was to describe the clinical, microbiological, radiological, and histopathological data of TB in children. A 7-year retrospective and descriptive cohort study that included 127 patients under 18 years of age with diagnosis of active TB was conducted from 2011 to 2018 in a pediatric hospital. Tuberculosis was microbiologically confirmed using Ziehl–Neelsen (ZN) staining, culture or polymerase chain reaction (PCR) in a total of 94 (74%) cases. Thirty-three cases were defined as probable TB based on tuberculin skin test result and epidemiological evaluation. The TB forms found were lymph node (39.3%), bone (15.7%), lung (13.6%), and meningeal TB (8.6%). The most common symptoms were fever (48.8%) and adenopathy (45.6%). History of contact was established in 34.6%. Positive ZN staining (sensitivity 30%) and culture (sensitivity 37%) were found in 29% and 37.7% of subjects, respectively. About 64.5% depicted abnormal chest X-ray. Xpert MTB/RIF® (PCR) was positive in 9.4% and biopsy was compatible in 52.7% of these samples. It is fundamental to have laboratory and epidemiological evaluation that support the diagnosis of the disease in children and thus, define its management; since, in most cases, early microbiologic confirmation is lacking.

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Mailoa, Meigy Nelce, Edir Lokollo, Dessyre Marlen Nendissa, and Pavita Indriani Harsono. "Microbiological and Chemical Characteristics of Smoked Tuna." Jurnal Pengolahan Hasil Perikanan Indonesia 22, no. 1 (April 30, 2019): 89. http://dx.doi.org/10.17844/jphpi.v22i1.25882.

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Smoked fish is traditionally processed by fish through an open process of hot smoked. Smoked fish process in Indonesia, especially in Maluku, is still carried out traditionally with capital and small business scale so that the use of tools is still simple, besides the sanitation and hygiene of handling and processing are still low. The raw material used in this study is smoke tuna loin produced by Dusun Air Manis,Laha village. This study aims to determine the microbiological and chemical quality of the smoked fish that was produced from Dusun Air Manis, Laha village. The test parameters carried out consisted of microbiological tests: 1) Total microbes on frozen tuna loin, washing water, immersion water, bamboo, smoked racks and smoked tuna, 2) Escherichia coli test on frozen tuna loin, washing water, soaking water, smoked tuna, 3) Salmonella test on smoked tuna. Chemical analysis of smoked tuna included moisture and pH. The results showed that the total plate count (TPC), Escherichia coli and Salmonella on tuna loin smoke still met microbiological standards according to SNI 2725: 2013. Moisture of smoked tuna was 59% and still meets the quality standard according to SNI 2725: 2013 which is a maximum of 60%. The pH of smoked tuna was 5.8.

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Domnina, Yu M., V. V. Suslov, N. E. Grammatikova, and S. A. Kedik. "Microbiological Estimation of Nasal Spray Containing Naltrexone Hydrochloride." Drug development & registration 9, no. 4 (November 26, 2020): 116–20. http://dx.doi.org/10.33380/2305-2066-2020-9-4-116-120.

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Introduction. In order to standardize the quality indicators during the expected shelf life of the developed drug naltrexone hydrochloride in the form of a nasal spray containing a high concentration of poloxamer and benzalkonium chloride as a preservative, a microbiological study was carried out. The possibility of using the membrane filtration method for testing prototypes recommended by the State Pharmacopoeia XIV.Aim. Study and selection of test conditions for the «microbiological purity» indicator of nasal spray samples containing naltrexone hydrochloride.Materials and methods. As an object of research, a naltrexone hydrochloride nasal spray was used. When analyzing the microbiological purity, the membrane filtration method recommended in the State Pharmacopoeia XIV.Results and discussion. As part of the study, it was found that the samples of the drug meet the requirements for a microbiological indicator for drugs of category 2. Testing the suitability of the method for samples of the dosage form showed that the antimicrobial effect of the drug was completely removed by washing the filter, which was proved by inoculation of indicator test microorganisms, the quantitative and qualitative nature of growth, which did not differ from the control without the drug.Conclusion. As a result of the studies carried out, the optimal test conditions for the «Microbiological purity» indicator for the nasal spray containing naltrexone hydrochloride were selected and substantiated.

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Ardhany, Syahrida Dian, Susi Novaryatiin, Mohammad Rizki Fadhil Pratama, and Zulkhurnain Utar. "IRRITATION TEST OF BAWANG DAYAK (Eleutherine bulbosa(Mill.) Urb.) EXTRACT CREAM WITH HUMAN PATCH TEST METHOD." Jurnal Farmasi Sains dan Praktis 7, no. 1 (May 1, 2021): 74–80. http://dx.doi.org/10.31603/pharmacy.v7i1.4854.

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Topical agents indicated for the treatment of acne have the potential to cause irritation or allergic contact dermatitis. This study investigates the irritancy potential of anti-acne cream of bawang dayak (Eleutherine bulbosa (Mill.) Urb.) previously tested for microbiological effectiveness with the lowest concentration of 5% and the highest concentration of 20%. The method used in this study is the human patch test. A total of 20 volunteers were recruited for the patch test study, testing the cream. The result showed that all volunteers did not experience irritation both in the 5% or 20% bawang dayak extract cream formulations. However, the interview results were found that some volunteers experienced a slight itching without any significant skin adverse reactions on the cream application. Therefore, based on these initial findings it can be safely concluded that the cream of bawang dayak does not cause significant skin adverse reaction and good enough for further development for anti-acne cream dosage form.

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Blodgett, Robert J., and Anthony D. Hitchins. "Evaluating Presence/Absence of Target Microbes in Microbiological Tests." Journal of AOAC INTERNATIONAL 83, no. 6 (November 1, 2000): 1429–34. http://dx.doi.org/10.1093/jaoac/83.6.1429.

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Abstract A typical qualitative microbiological method performance (collaborative) study gathers a data set of responses about a test for the presence or absence of a target microbe. We developed 2 models that estimate false-positive and false-negative rates. One model assumes a constant probability that the tests will indicate the target microbe is present for any positive concentration in the test portion. The other model assumes that this probability follows a logistic curve. Test results from several method performance studies illustrate these estimates.

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Moreno, Andréia De Haro, and Hérida Regina Nunes Salgado. "Microbiological Assay for Ceftazidime Injection." Journal of AOAC INTERNATIONAL 90, no. 5 (September 1, 2007): 1379–82. http://dx.doi.org/10.1093/jaoac/90.5.1379.

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Abstract A simple, sensitive, and specific biodiffusion assay for the antibacterial ceftazidime was developed using a strain of Staphylococcus epidermidis (ATCC 12228) as the test organism. Ceftazidime was measured in powder for injection at concentrations ranging from 100 to 400 g/mL. The calibration graph for ceftazidime was linear (r2 = 1), and the method validation showed that it was precise (relative standard deviation = 0.415) and accurate. The results obtained by biodiffusion assay were statistically calculated by linear parallel model and by means of regression analysis and were verified using analysis of variance. It was concluded that the microbiological assay is satisfactory for in vitro quantification of the antibacterial activity of ceftazidime in pharmaceuticals.

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Bose, Sanchali, Senthil Kumar Ponnusamy, and Pooja G. "A Preliminary Study on Detection of Serogenic E.coli in Fishes to Conduct a Microbial Assessment of Coastal Water." ECS Transactions 107, no. 1 (April 24, 2022): 3863–73. http://dx.doi.org/10.1149/10701.3863ecst.

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Escherichia coli is a bacterial pathogen mostly responsible for gut infections and related serious illnesses. There have been some studies in the past which detected the presence of E.coli in edible fishes. The diarrheagenic E.coli strains are mainly responsible for such health issues. E.coli is an indicator of the microbiological quality of water as it indicates faecal contamination. In this paper, it is intended to understand the microbiological quality of coastal water inhabited by fish. The edible fishes consumed along the Vishakhapatnam coast were subjected to various tests like Indole production test, Citrate utilization test, Urease test to confirm the presence of E.coli. It was thereby confirmed through Polymerase Chain reaction that Indian Mackerel and Indian Salmon were infected with Sta (Heat stable toxin) serotype and SLT2 (Shiga toxin) respectively. This indicates the poor microbiological quality of the coastal water along with poor handling practices.

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Raghuprakash P, Gowrav M P, Gangadharappa H V, and Hemanth Kumar S. "Rapid microbiological testing method." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (July 22, 2020): 3927–32. http://dx.doi.org/10.26452/ijrps.v11i3.2582.

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Classical microbiological methods currently have unacceptably long cycle times. Rapid microbiological strategies are accessible within the marketplace for about 10 years and are mostly used in the clinical laboratory and in food industries. However, their reapplication in the pharma industry has wide range of advantage. A comparison with ancient strategies to be conjointly performed. During this review, data concerning the validation of RMM strategies described within the document was given in addition as proof of the issue of validation of those strategies. A comparison with ancient strategies is additionally mentioned. This data is beneficial to the industries and in the labs which will doubtless be adopted. These strategies for microorganism free products. This methodology for microorganism identification will be delicate, accurate and fast. How the laboratory should be maintained for carrying out different tests, there should be good hygienic condition maintained. This article also includes different methods for identifying bacteria which is present in drug products as well as the material which are used for doing test. Presence of bacteria may affect the activity of drug product and bio availability may decreases and potency the drug may loss. How the laboratory should be maintained for carrying out different tests, there should be good hygienic condition maintained.

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V, Manish kuptha, and Ragavendiran Rao B. "Justification of Profitable Test Kit for Microbiological Viewing of Antimicrobials in Rooster Eggs." International Journal of Veterinary Science 1, no. 1 (April 25, 2015): 15–17. http://dx.doi.org/10.14445/24550868/ijvs-v1i1p105.

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Fagbamila, I. O., S. S. Ngulukun, S. S. Ardzard, N. Sati, O. T. Ajayi, P. I. Ankeli, Y. Akalusi, et al. "Validation of Commercial Test Kit for Microbiological Screening of Antimicrobials in Chicken Eggs." Research Journal of Veterinary Sciences 5, no. 3 (March 1, 2012): 75–80. http://dx.doi.org/10.3923/rjvs.2012.75.80.

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