Academic literature on the topic 'Temperate native pasture'

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Journal articles on the topic "Temperate native pasture"

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Pearson, C. J., R. Brown, W. J. Collins, K. A. Archer, M. S. Wood, C. Petersen, and B. Bootle. "An Australian temperate pastures database." Australian Journal of Agricultural Research 48, no. 4 (1997): 453. http://dx.doi.org/10.1071/a96095.

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A census of pasture types and their composition and attributes (e.g. purpose and carrying capacity) was carried out throughout southern Australia from June to October 1994. This paper describes the survey process and subsequent creation of an Australian temperate pastures database. Data were created for 562 local government areas (LGAs) from ‘desk estimates’ by trained agriculturists. They identified about 2500 pasture types, which were grouped into 120 standardised pasture categories. Some findings from the data are identified, namely the high relative importance on an area basis of unimproved native pastures, the importance of weedy species (e.g. Vulpia), and the marked decline with increasing rainfall in importance of annual medics compared with subterranean clover. This paper provides examples of the data and their use for assessment of the regional economic impact of increases in productivity of pasture species.
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Vere, D. T., R. E. Jones, and M. H. Campbell. "The economics of temperate pasture systems on the central and southern Tablelands of New South Wales." Rangeland Journal 23, no. 2 (2001): 159. http://dx.doi.org/10.1071/rj01003.

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Pastures are the basis of most forms of agricultural production on the New South Wales central and southern tablelands. Pastures occupy the bulk of the region's landmass and pasture-based livestock production annually contributes more than three-quarters of the regional gross value of rural production. Throughout the region, there is substantial variation in pasture composition, ranging from high quality introduced perennial grasses and legumes to pastures comprising mainly low quality native species. This paper examines the economics of the main categories of temperate pastures over a range of soil fertility-rainfall environments on the south-eastern tablelands areas of New South Wales. Using a linear programming model and discounted development budgets, the results demonstrate the strong influence of the environment on the economics of the individual pasture systems. The highest economic returns in both the short and longer-terms were to the introduced perennial grass pastures in most of the environments. Pastures based on introduced legumes and the high quality native species also generated sound economic returns, although there are recognised problems with the persistence of the legume pastures. Over time, the returns to the better quality native pastures compare favourably with the introduced legumes and are better suited to acidic soils than the perennial grasses. Low quality native species produced relatively poor economic returns in all environments and unfortunately, are the main pasture type in the region's less favourable environments.
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Eyles, Alieta, Garth Coghlan, Marcus Hardie, Mark Hovenden, and Kerry Bridle. "Soil carbon sequestration in cool-temperate dryland pastures: mechanisms and management options." Soil Research 53, no. 4 (2015): 349. http://dx.doi.org/10.1071/sr14062.

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Permanent pastures, which include sown, native and naturalised pastures, account for 4.3 Mha (56%) of the national land use in Australia. Given their extent, pastures are of great interest with respect to their potential to influence national carbon (C) budgets and CO2 mitigation. Increasing soil organic C (SOC) mitigates greenhouse gases while providing other benefits such as pasture productivity, soil health and ecosystem services. Several management approaches have been recommended to increase C sequestration in pasture-based systems; however, results have proved variable and often contradictory between sites and years. Here, we present an overview of the processes and mechanisms responsible for C sequestration in permanent pastures. In addition, we discuss the merits of traditional and emerging pasture-management practices for increasing SOC in pastures, with a focus on dryland pasture systems of south-eastern Australia. We conclude by summarising the knowledge gaps and research priorities for soil C-sequestration research in dryland pastures. Our review confirms that soils under a range of pasture types have considerable potential for sequestration of atmospheric CO2 in Australia, and that the magnitude of this potential can be greatly modified by pasture-management practices. Although the shortage of long-term studies under Australian conditions limits our ability to predict the potential of various management approaches to sequester soil C, our review indicates that prevention of erosion through maintenance of groundcover and adoption of options that promote deep C sequestration are likely to confer broad-scale maintenance or increases in SOC in pasture soils over a decade or longer. We acknowledge that the evidence is limited; therefore, confidence in the recommended practices in different locations and climates is largely unknown.
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Lodge, G. M. "Studies of soil seedbanks in native and sown pastures in northern New South Wales." Rangeland Journal 23, no. 2 (2001): 204. http://dx.doi.org/10.1071/rj01007.

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Total and germinable soil seedbanks (litter and soil) were studied for a native pasture and three sown pastures (dominated by Phalaris aquatica L. cv. Sirosa) in northern New South Wales from 1993 to 1996. Soil core samples were taken from continuously grazed plots for both pasture types and two oversown treatments in the native pasture and from a spring-autumn rest treatment at the sown pasture sites. At each site above ground herbage mass was also estimated regularly as part of the Temperate Pasture Sustainability Key Program. For all sites and treatments, the proportion of germinable seeds as a percentage of the total (dormant and germinable) seedbank ranged from 1–26% for the native pasture and 1–39% for the sown pastures. Germinable seed numbers ranged from 280 to 26,110 seeds per m2, while total seedbank numbers were from 6700 to 178,360 seeds per m2. Native pasture herbage mass was dominated by native perennial grasses, but seeds of these species were less than 20% of the total seed bank in all treatments in 1994 and 1995. At the sown pasture sites, most of the germinable (51–92%) and total (65–97%) seedbanks were either barnyard grass (Echinochloa crus-galli (L.) Beauv, annual ryegrass (Lolium rigidum Gaudin), subterranean clover (Trifolium subterraneum L.) or wireweed (Polygonum aviculare L.). Since seeds of annuals and other forbs generally dominated both the total and germinable seedbanks of these perennial grass-based pastures, these species were likely to increase over time. Seeds of the sown perennial grass Phalaris aquatica L, cv. Sirosa were less than 1% of the total seedbanks in pastures sown in 1990 and less than 3% of those sown in 1979. With above average summer rainfall at the native pasture site in 1996 and prolific growth of redgrass, seeds of this species were 38–63% of the total and 11–29% of the germinable seedbank in May 1996. Except at this site and time, the species composition of the total and germinable seedbanks did not generally reflect the dominance of the above ground herbage mass of these pastures by perennial grasses.
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Barnes, Phoebe, Brian R. Wilson, Mark G. Trotter, David W. Lamb, Nick Reid, Terry Koen, and Leopold Bayerlein. "The patterns of grazed pasture associated with scattered trees across an Australian temperate landscape: an investigation of pasture quantity and quality." Rangeland Journal 33, no. 2 (2011): 121. http://dx.doi.org/10.1071/rj10068.

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Scattered paddock trees occur across agricultural landscapes in Australia. However, in the temperate regions of Australia their numbers are rapidly declining and they may be lost across much of the landscape in 200 years. Here we examined the spatial distribution of green (GDB), senescent (SDB) and total (TDB) dry pasture biomass, and nutrient status of the GDB fraction around scattered Eucalyptus trees on three parent materials (basalt, granite and meta-sediment) in native and sown pastures across a range of grazed temperate landscapes in northern New South Wales. We used a combination of destructive harvests and a handheld active optical canopy reflectance sensor (AOS) with an integrated GPS to examine pasture biomass around scattered trees. The harvested pasture biomass data indicated that under grazed conditions the presence of scattered trees did not introduce significant radial trends in TDB or GDB out to a distance of 3.5 canopy radii regardless of tree species or parent material. The red and near-infrared reflectance-based Normalised Difference Vegetation Index (NDVI), as measured by the AOS, did indicate a consistent azimuthal trend with larger GDB on the southern side of the tree and lower GDB on the northern side in the native pasture. However, this observation must be qualified as the regression coefficient for the relationship between NDVI and GDB was significant but weak (best r2 = 0.42), and SDB reduced its predictive capacity. We also found a higher percentage of GDB under the canopy than in the open paddock. We suggest that the combination of these results may indicate higher grazing pressure under trees than in the open paddock. Pasture nutrient concentration (P, K and S) was higher in both native and sown pastures beneath the tree canopy compared with the open paddock. This study indicates that, in this temperate environment, scattered trees do not adversely affect pasture production, and that they can improve some pasture nutrients.
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Vickery, P. J., M. J. Hill, and G. E. Donald. "Satellite derived maps of pasture growth status: association of classification with botanical composition." Australian Journal of Experimental Agriculture 37, no. 5 (1997): 547. http://dx.doi.org/10.1071/ea97014.

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Summary. Spectral data from the green, red and near-infrared bands of Landsat MSS and Landsat TM satellite imagery acquired in mid-spring were classified into 3 and 6 pasture growth classes respectively. The classifications were compared with a site database of botanical composition for the Northern Tablelands of New South Wales to examine the association between spectral growth class and pasture composition. Pastures ranged in composition from unimproved native perennial grasses through semi-improved mixtures of native and naturalised grasses and legumes to highly improved temperate perennial grasses and legumes. For 3 years of MSS data, the fast growth class had a mean botanical composition of about 80% improved perennial grass and 0% native; medium growth class averaged 46% improved perennial grass and 14% native; while the slow growth class had about 60% native and 1% improved perennial grass when averaged over 3 years of MSS data. For the 6 class TM data from a single year, a predictive logistic regression of cumulative probability was developed for percentage of ‘very fast’ growth pixels and ordered 10 percentile categories of improved perennial grass or native grass. Differences in patch characteristics between classes with MSS disappeared with TM reclassified to the same 3 class level. Most probable pasture type was inferred from 3 class MSS and TM data using Bayesian probability analysis. The resulting maps were similar in general appearance but detail was better with the TM data. The pasture growth classification identified highly improved perennial grass pastures and native pastures but sensitivity to intermediate pasture types was poor. Future improvement will come from direct measurement of biophysical characteristics using vegetation indices or inversion of reflectance models.
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Garden, D. L., G. M. Lodge, D. A. Friend, P. M. Dowling, and B. A. Orchard. "Effects of grazing management on botanical composition of native grass-based pastures in temperate south-east Australia." Australian Journal of Experimental Agriculture 40, no. 2 (2000): 225. http://dx.doi.org/10.1071/ea98010.

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Grazing management strategies to alter botanical composition of native pastures were investigated at 4 locations in the high rainfall zone of south-east Australia, including Tasmania. These studies were conducted as part of the Temperate Pasture Sustainability Key Program, which evaluated the effects of grazing management on a wide range of pasture types between 1993 and 1996. Pastures in this study were based on Aristida ramosa/Bothriochloa macra, Microlaena stipoides–Austrodanthonia spp. or Themeda triandra–Austrodanthonia spp. Seasonal rests, increased grazing pressure in spring, mob stocking and cutting for hay were compared to continuous grazing at all sites. In addition, specific local treatments were tested at individual sites. Changes in composition resulting from the treatments were minimal at most sites. This may have been due to a combination of the inherent stability of the pastures, the relatively short duration of the experiments, and the drought conditions experienced, which minimised differences between treatments. Some strategies to alter composition of natural pastures are suggested. In the Aristida–Bothriochloa pasture there was a general decrease in Aristida and an increase in Bothriochloa, which was largely unaffected by the type of grazing management applied. The combination of drought conditions and increasing grazing pressure was sufficient to alter composition without specific management strategies being necessary. In the Themeda–Austrodanthonia pasture, resting in spring, 12-month rests or cutting for hay (which involved a spring rest) allowed Themeda to increase in the pasture. The Microlaena–Austrodanthonia pastures were very stable, especially where annual grass content was low. However, certain treatments allowed Microlaena to increase, a result which is regarded as being favourable. The major effects in these latter pastures were on undesirable species. Vulpia spp. were reduced by resting in autumn and increased spring grazing pressure, while Holcus lanatus was increased dramatically by resting in spring and was also increased by resting in autumn or winter, but only when conditions were suitable for growth of this species. In many cases, treatment differences were only expressed following recovery from drought, showing that timing of grazing management to achieve change is critical.
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Vere, D. T., R. E. Jones, and M. H. Campbell. "Long-term change in the economic productivity of four major pasture categories on the south-eastern Tablelands of New South Wales." Rangeland Journal 23, no. 2 (2001): 148. http://dx.doi.org/10.1071/rj01002.

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The perception of change or decline in the productivity of temperate pastures in south-eastern Australia is an important concern to livestock producers and pasture scientists. Much of this concern relates to reductions in the proportions of desirable species in the composition of pasture systems as a result of increased soil and weed problems. The purpose of this paper is to investigate trends in the long-term economic productivity of four categories of temperate pastures (all introduced pastures, introduced perennial grasses, introduced legumes and all native pastures) on the central and southern tablelands of New South Wales. The results provide evidence of economic productivity decline in the all introduced pastures category in relation to sheep production, but this has been due to productivity decline in the dominant legume component of the introduced pastures. In contrast, there has been strong growth in the economic productivity of the introduced perennial grass pastures. Abnormally high beef cattle numbers in the mid-1970s appear to have created an illusion of high productivity and subsequent decline in all introduced pastures. In contrast, the economic productivity of the native pastures which are the bulk of the region's grazing areas, has fallen substantially.
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Whalley, R. D. B., D. A. Friend, P. Sanford, and M. L. Mitchell. "Evaluation of native and introduced grasses for low-input pastures in temperate Australia: rationale and scope." Rangeland Journal 27, no. 1 (2005): 1. http://dx.doi.org/10.1071/rj05004.

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The historical approach to pasture improvement in the high rainfall zone of temperate Australia has been to add introduced herbaceous legumes and to replace perennial native grasses with introduced species requiring high inputs of fertiliser for maintenance. The application of this high-input approach on land with low capability has lead to the loss of perennial grasses, erosion, soil acidification and increasing salinity on the lower slopes. This model of pasture improvement has not been successful on the margins of the wheat belt and in semi-arid regions. The Native and Low-input Grasses Network (NLIGN) was established in 1996 to coordinate research on grasses suitable for land with low capability and for semi-arid regions. The NLIGN multi-site evaluation project was initiated to test promising lines (accessions) of native and introduced grasses for low-input pastures at eight sites across southern Australia. The broad objective of the project was to identify native and/or introduced perennial grass lines that had possible commercial potential for low-input pastures. This objective was achieved in an initial 3-year evaluation phase, which began in 1998 and tested lines for persistence, production and palatability.
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King, K. L., and K. J. Hutchinson. "Pasture and grazing land: assessment of sustainability using invertebrate bioindicators." Australian Journal of Experimental Agriculture 47, no. 4 (2007): 392. http://dx.doi.org/10.1071/ea05270.

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Impacts of practices frequently used to manage Australian pastures are reviewed with the aim of determining which groups are responsive to changes in grazing regime, fertiliser use, pasture types, tree clearing, pesticide use, liming and irrigation. Invertebrate groups sensitive to pasture management regimes may be potential candidates for use as bioindicators of ecological sustainability of these pasture types. This review concentrates on the more intensively utilised temperate pastures of southern Australia, as very little work has been done on the impact of the grazing animal and pasture management on invertebrate fauna on the extensive rangelands of the arid and semiarid zones. Background to the relative importance of invertebrates in the functioning of the pasture ecosystem is given. This has culminated in the construction of food webs for two temperate perennial pastures (an unfertilised, native and a fertilised, sown pasture) at Armidale, NSW, for which there is comprehensive data available. Invertebrate bioindicators of pasture sustainability emerged from the consideration of grazing lands and invertebrate responses. Currently, only four groups would seem to be likely candidates as bioindicators of sustainability of pastures. These are soil nematodes, earthworms, protozoa and Collembola. The main difficulty in monitoring these groups is that it requires specialist expertise, and services provided by commercial laboratories for routine biological soil tests are still in their infancy. There are gaps in our knowledge of how invertebrate fauna react to the pressing issues of soil acidity and salinity.
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Dissertations / Theses on the topic "Temperate native pasture"

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Wallace, Richard Paul, and n/a. "Effects of trees on temperate native pasture productivity." University of Canberra. Applied Science, 1999. http://erl.canberra.edu.au./public/adt-AUC20061113.160245.

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The goal of this work was to quantify the effects of eucalypt woodland blocks on the productivity of native pastures. This research was conducted on the Southern Tablelands of New South Wales. Tree planting or retention is seen by many as an important tool in addressing the problems of soil degradation resulting from clearing and pasture improvement that threaten the sustainability of pasture systems. In particular these are dry land salinity and erosion, both of which affect large areas of agricultural lands in the south east of Australia. Whilst native tree cover remains over substantial portions of Australian pasture lands, mainly on steeper slopes and poorer soils, little has been done to measure the effects of trees on pasture productivity and soil fertility on the Southern Tablelands. Previous studies in other areas have shown a range of effects�from facilitation to inhibition�of pasture growth in the presence of trees. Soil fertility beneath trees has been shown by a number of workers to be elevated in comparison with situations in the open. Given that the range of effects may be highly site dependent, application of results from one area to another may not be valid. Thus it is necessary to measure tree effects on a regional scale if results are to be reliable. Pasture productivity was assessed over a two year period on four sites in the vicinity of Bungendore, New South Wales. A pair of plots was selected on each site, one plot in a block of eucalypt woodland, and the other nearby in an exposed, open situation. Plots were chosen to be as similar to each other as possible with the exception of tree cover. Treed plots had a tree basal area of between 10 and 20 m2 ha-1 and plots had an area of 900 m2. Two of the sites were on granitic soils and had a tree cover consisting predominantly of Eucalyptus pauciflora. The remaining two sites were on soil derived from sedimentary rocks with tree cover consisting mainly of E. mannifera, E. dives and E. melliodora. Perennial native pasture species present were similar across all sites, although their relative contributions to standing biomass varied between sites. As the plots were grazed during the period of measurement, productivity and offtake were measured seasonally using exclosure cages on each plot. Pasture standing biomass was assessed using the comparative yield technique. Microclimate was monitored in each plot by automatic weather stations. Soil moisture to a depth of 45 cm was measured by time domain reflectometry using permanent probes in each plot. Ten additional survey plots on each site, covering the range of tree basal area from 0 - 30 m2 ha-1, were assessed each season in the second year for standing biomass, soil fertility and pasture quality; expressed by nitrogen content and dry matter digestibility. Pasture floristics were measured using the dry-weight-rank method. These additional plots were chosen to be as representative of the paddocks as possible. Over the two years that productivity was measured, it was found to be higher under trees than in the open. This was predominantly due to higher winter and spring growth within treed plots. Grazing offtake was also found to be higher under trees, partly accounting for lower standing biomass found in the treed plots. Wind run, evapotranspiration and photosynthetically active radiation were all reduced by the presence of trees. Beneficial effects of shelter from winds may largely explain the higher productivity observed in the treed plots, and could outweigh negative effects of below ground competition and radiation interception by tree canopies at low to moderate tree densities. Soil moisture was not affected by the presence of trees. Soil fertility also did not differ between treed and open plots nor was there any difference in pasture nitrogen content or dry matter digestibility. On the sites where soils were derived from sedimentary rocks, pasture floristics were found to be related to tree basal area. Themeda ausfralis biomass was negatively related to tree basal area, and was partially replaced by large tussock species such as Poa sieberiana and Chionochloa pallida. A reduction of pasture quality resulted, particularly as the latter species is not grazed to any significant extent. Given the desirability of having deep rooted perennial components in grazing lands, the results of this study indicate that it may be possible to utilise trees to assist in preventing or reducing a range of adverse environmental consequences arising from agricultural activities, without unduly compromising pasture productivity. Additionally, the wide range of environmental conditions provided by a mix of treed and open pasture promotes a higher degree of heterogeneity of the herbaceous layer. This may assist in maintaining productivity over a greater range of climatic conditions than would be the case with a more homogeneous pasture.
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Johnston, William Henry, University of Western Sydney, of Science Technology and Environment College, and School of Environment and Agriculture. "The role of Eragrostis curvula (Schrad.) Nees. complex in temperate pastures in southeastern Australia." THESIS_CSTE_EAG_Johnston_W.xml, 2003. http://handle.uws.edu.au:8081/1959.7/29.

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This thesis examines the hypothesis that, in southern New South Wales and northeast Victoria, Australia, palatable taxa of E. curvula offer advantages that complement those of the species that are traditionally sown in temperate pastures in a landscape context.This hypothesis was based on a review of literature showing that, prior to European settlement, the vegetation, the landscape and the climate were broadly in balance, and the wateruse pattern of the vegetation of southeastern Australia resulted in water being used more-or-less completely by the end of summer. This maximised the capacity of the soil to take up and store water during autumn and winter.Three grazing experiments and one spaced-plant species evaluation study were used to assess the role of summer-growing, C4 Eragrostis curvula in pastures in the temperate zone of southeastern Australia.Issues relating to pasture production and the productivity of wool-growing sheep were investigated. Factors affecting the sustainability of the pastures and their potential on and off site impacts were emphasised.Modelling was used to explore issues of water use, arising from the grazing experiments. It is concluded that the persistence, production, water use patterns, and the adaptability of palatable varieties of E. curvula make it a useful and complementary addition to the range of species that are currently available for use as sown pastures in southern Australia.
Doctor of Philosophy (PhD)
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Joseph, Tony. "The response of photosynthesis and respiration of a grass and a native shrub to varying temperature and soil water content." Thesis, University of Canterbury. School of Biological Sciences, 2011. http://hdl.handle.net/10092/6685.

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In New Zealand, native shrubs are considered an important potential carbon-sink in disturbed or abandoned land (e.g., pastoral land that is unsustainable for long-term pastoral agriculture). However, the impact of varying environmental drivers on carbon uptake from photosynthesis and carbon loss from respiration of a developing shrubland remains uncertain. In this study, the effects of both temperature and soil water content (θ) on photosynthesis and respiration were examined under controlled growth cabinet and field conditions in a pasture grass and the native shrub, kānuka (Kunzea ericoides var. ericoides). The purpose of the investigation was to assess the combined impacts of varying temperature and θ on canopy processes and to disentangle the effects of θ on photosynthesis and respiration for the two different plant types. A controlled growth cabinet study (Chapter 2) showed that θ had a greater effect on the short-term temperature response of photosynthesis than the temperature response of respiration. The optimum value of θ for net photosynthesis was around 30 % for both kānuka and the grass. Statistical analysis showed that the temperature sensitivity of photosynthetic parameters was similar for both plant types, but the sensitivity of respiratory parameters was different. Reduction in θ induced an inhibition of photosynthetic capacity in both plant types. The response of respiratory parameters to θ was not related to substrate limitations, however available evidence suggests that it is likely to be a species dependent plant mechanism in regulating the cost of maintenance due to reduced photosynthate assimilation and decreasing energy supply to support the activity of respiratory enzymes. Results obtained from a field study (Chapter 3) showed that photosynthesis and respiration in the grass and kānuka were sensitive to seasonal changes in temperature and θ. Photosynthetic parameters showed little acclimation following changes in seasonal growth conditions. In contrast, respiratory parameters tended to acclimate more strongly. Respiratory acclimation to multiple environmental conditions was characterised by changes in temperature sensitivity and a shift in the response of respiration to temperature, demonstrating the involvement of both ‘Type I’ and ‘Type II’ acclimation in both plant types. The results from controlled growth cabinet and field studies were used to drive a leaf level model that integrates the responses of photosynthesis and respiration to changes in temperature and θ and incorporates acclimation using variable photosynthetic and respiratory parameters (Chapter 4). This model was used to estimate the annual canopy carbon exchange of the grass and kānuka in response to seasonal changes and to predict changes in canopy carbon exchange under varying future climate change scenarios. The model highlighted the importance of considering seasonally-acclimated parameters in estimating canopy carbon exchange of both plant types to concurrent changes in multiple environmental variables. The overall results support the conclusion that understanding the combined effects of environmental variables on canopy processes is essential for predicting canopy net carbon exchange of a pasture-shrub system in a changing global environment. It has been shown here that the rate of increase in photosynthesis with increasing θ is greater than that of respiration which results in a progressively greater apparent carbon gain at moderate values of θ. Moreover, the impact of lower values of θ, which reduced the apparent sensitivity of respiration to temperature, may effectively decrease the rate of respiration during warmer summer months and enhance thermal acclimation via downregulation of respiration. Therefore, considering the influence of soil water conditions on the temperature sensitivity of photosynthetic and respiratory model parameters has important implications for precisely predicting the net carbon exchange of a pasture-shrub system.
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Conference papers on the topic "Temperate native pasture"

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Wang, Chengyang, Qudus Hamid, Jessica Snyder, Halim Ayan, and Wei Sun. "A Novel Automation System for Microplasma Surface Patterning and Biologics Printing." In ASME/ISCIE 2012 International Symposium on Flexible Automation. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/isfa2012-7106.

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In the field of tissue engineering, regenerative medicine, and life sciences, the topological biochemical cues regulate cell attachment and alignment within the construct. In a native biological system, these cues are inherent. However, most of the biological materials utilized in the fabrication of tissue construct do not possess the appropriate cues required to develop an architecture to support the cell attachment and growth of a functional tissue Therefore the ability to manipulate structural and biochemical cues plays an important role in biofabrication process, and it is a key element to evaluate a engineered cellular model. Plasma surface functionalization and biologics printing have been investigated and validated as two effective techniques to guide cell functions by creating microenvironments. The objective of this work is to develop a novel dual functional platform for freeform microplasma surface patterning and biologics printing process as well as to study the underlying process science and the process induced cellular functions. The microplasma jet system was assembled by two parts. The upper part is a plastic NPT connector surrounding an extending high voltage copper electrode. The lower part is a dielectric Pasteur pipette connected with a capillary micro-scale nozzle tip. The lower part is interchangeable and the diameter of the tip ranges from 50 μm to 1 mm. With up to 20 kV output capability, a high-voltage power supply was connected to the copper electrode through the NPT connector which also served as gas inlet. A high-voltage probe linked to an oscilloscope is used to monitor the real time voltage. The whole microplasma jet system was set up on automation platform, which allows X-Y-Z motion control and switch control. This integrated system operates at atmospheric pressured environment. All tissue constructs could be fabricated at room temperature without the use of a mask. Clear polystyrene microplates were used as plasma treatment substrates. After O2-He mixed microplasma treatment, 7F2 mouse osteoblastic cells were cultured in the microplates for cell biology studies. We demonstrated the capability of our dual functional platform by applying microplasma in the polystyrene wells and control group (without any treatment) in other wells of the same microplate substrate. The results show that the microplasma treatment changed the surface properties and improved cell attachment. This dual functional freeform system allows for surface patterning and printing of cells, proteins, growth factors, etc. to fabricate three-dimensional tissue constructs.
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