Relevant bibliographies by topics / Targeted integration

Academic literature on the topic 'Targeted integration'

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Published: 9 March 2023
Last updated: 5 October 2024

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Journal articles on the topic "Targeted integration"

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Amoils, Shannon. "Targeted integration." Nature Reviews Microbiology 4, no. 2 (February 2006): 87. http://dx.doi.org/10.1038/nrmicro1357.

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Tabernero, J., and F. J. Ramos. "305 Integration of targeted therapies." European Journal of Cancer Supplements 7, no. 2 (September 2009): 74. http://dx.doi.org/10.1016/s1359-6349(09)70258-7.

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Shi, Zhaoying, Dandan Tian, Huhu Xin, Jingru Lian, Xiaogang Guo, and Yonglong Chen. "Targeted integration of genes inXenopus tropicalis." genesis 55, no. 1-2 (January 2017): e23006. http://dx.doi.org/10.1002/dvg.23006.

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Bhatt, Shivam, and Ronald Chalmers. "Targeted DNA transposition in vitro using a dCas9-transposase fusion protein." Nucleic Acids Research 47, no. 15 (June 25, 2019): 8126–35. http://dx.doi.org/10.1093/nar/gkz552.

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Abstract Homology-directed genome engineering is limited by transgene size. Although DNA transposons are more efficient with large transgenes, random integrations are potentially mutagenic. Here we present an in vitro mechanistic study that demonstrates efficient Cas9 targeting of the mariner transposon Hsmar1. Integrations were unidirectional and tightly constrained to one side of the sgRNA binding site. Further analysis of the nucleoprotein intermediates demonstrated that the transposase and Cas9 moieties can bind their respective substrates independently or in concert. Kinetic analysis of the reaction in the presence of the Cas9 target–DNA revealed a delay between first and second strand cleavage at the transposon end. This step involves a significant conformational change that may be hindered by the properties of the interdomainal linker. Otherwise, the transposase moiety behaved normally and was proficient for integration in vitro and in Escherichia coli. Specific integration into the lacZ gene in E. coli was obscured by a high background of random integrations. Nevertheless, Cas9 is an attractive candidate for transposon-targeting because it has a high affinity and long dwell-time at its target site. This will facilitate a future optogenetic strategy for the temporal control of integration, which will increase the ratio of targeted to untargeted events.
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Hughes, A. J., R. K. C. Lin, D. M. Peehl, and A. E. Herr. "Microfluidic integration for automated targeted proteomic assays." Proceedings of the National Academy of Sciences 109, no. 16 (April 2, 2012): 5972–77. http://dx.doi.org/10.1073/pnas.1108617109.

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Stephens, Zachary, Daniel O’Brien, Mrunal Dehankar, Lewis R. Roberts, Ravishankar K. Iyer, and Jean-Pierre Kocher. "Exogene: A performant workflow for detecting viral integrations from paired-end next-generation sequencing data." PLOS ONE 16, no. 9 (September 22, 2021): e0250915. http://dx.doi.org/10.1371/journal.pone.0250915.

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The integration of viruses into the human genome is known to be associated with tumorigenesis in many cancers, but the accurate detection of integration breakpoints from short read sequencing data is made difficult by human-viral homologies, viral genome heterogeneity, coverage limitations, and other factors. To address this, we present Exogene, a sensitive and efficient workflow for detecting viral integrations from paired-end next generation sequencing data. Exogene’s read filtering and breakpoint detection strategies yield integration coordinates that are highly concordant with long read validation. We demonstrate this concordance across 6 TCGA Hepatocellular carcinoma (HCC) tumor samples, identifying integrations of hepatitis B virus that are also supported by long reads. Additionally, we applied Exogene to targeted capture data from 426 previously studied HCC samples, achieving 98.9% concordance with existing methods and identifying 238 high-confidence integrations that were not previously reported. Exogene is applicable to multiple types of paired-end sequence data, including genome, exome, RNA-Seq and targeted capture.
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Blumenschein, George R., and Roy S. Herbst. "Integration of Targeted Therapies in Gemcitabine Chemotherapy Regimens." Clinical Lung Cancer 4, no. 4 (January 2003): 217–23. http://dx.doi.org/10.3816/clc.2003.n.001.

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Volis, Sergei. "Species-targeted plant conservation: time for conceptual integration." Israel Journal of Plant Sciences 63, no. 4 (February 6, 2015): 232–49. http://dx.doi.org/10.1080/07929978.2015.1085203.

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To date, there have been only limited attempts to conceptually unify ex situ and in situ approaches as parts of an integrated conservation methodology. This paper is an attempt of such conceptual integration of existing approaches for the efficient conservation of rare and endangered plant species. My integration of available plant conservation biology literature is based on the idea that ecologically significant species genetic variation is of primary importance for plant conservation. This idea is used for providing guidelines about inventory of existing populations, sampling and propagating sampled material, and use of this material in species recovery actions.
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Sun, Duxin. "Nanotheranostics: Integration of Imaging and Targeted Drug Delivery." Molecular Pharmaceutics 7, no. 6 (December 6, 2010): 1879. http://dx.doi.org/10.1021/mp1003652.

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Montini, Eugenio, Daniela Cesana, Manfred Schmidt, Francesca Sanvito, Maurilio Ponzoni, Lucia Sergi Sergi, Fabrizio Benedicenti, et al. "Modeling the Genotoxicity of Viral Vector Integration in a Tumor Prone Hematopoietic Stem Cell Transplantation Model." Blood 108, no. 11 (November 16, 2006): 451. http://dx.doi.org/10.1182/blood.v108.11.451.451.

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Abstract Insertional mutagenesis represents a major hurdle to successful gene therapy and mandates for sensitive pre-clinical assays of genotoxicity. Cdkn2a−/ − mice are defective for p53 and Rb pathways, and are susceptible to a broad range of cancer-triggering genetic lesions. We developed an in-vivo genotoxicity assay, based on transplantation of Cdkn2a−/ − hematopoietic stem cells (HSCs), treated or not with prototypical retroviral (RV) and lentiviral (LV) vectors. In our rationale if RV or LV treatment is genotoxic, transplanted mice will show a significantly earlier tumor onset. Using this approach, we detected a dose-dependent acceleration in tumor onset in the mice transplanted with RV treated cells. We compared the RV and LV integration site distribution in pre-transplant cells and tumors from the transplanted mice. As expected, RV integrates close to gene promoters in pre-transplant cells and tumors. Moreover, we found that RV preferentially targeted genes encoding for transcription factors, kinases and genomic positions previously described as Common Integration Sites (CIS). CIS are genomic regions targeted at high frequency in tumors by retroviral integrations and probably map within or close to proto-oncogenes activated upon integration. Interestingly, in tumors, RV insertions at CIS and cell cycle genes were further enriched and associated to early lymphomagenesis. Remarkably, LV tested in the same conditions, did not show any tumor acceleration, and targeted CIS much less frequently than RV in pre-transplant cells or tumors, and did not show selection for integrations at any specific gene class. This is the first evidence that prototypic LV have low oncogenic potential, and provides a major rationale for their application to HSC gene therapy. To dissect the role in lymphomagenesis of the strong enhancers in the RV LTRs and the different integration site selection of each vector, we tested an RV with enhancer deleted LTRs (SIN RV) and a moderate promoter in internal position, an LV with a strong RV-like enhancer-promoter into the LTRs or in internal position. Our results show that: SIN RV shows reduced effect on lymphomagenesis acceleration with respect the conventional RV; LV with RV like LTRs treatment significantly accelerates lymphomagenesis, underlining important role of RV-LTRs in oncogenesis; LV with RV-like enhancer in internal position show a reduced genotoxicity. These data show that the type of genetic elements used (strong enhancers or moderate promoter) and their position in the vector genome (LTR or internal positions) significantly influence the vector safety profile. We are currently mapping the integration sites in pre-transplant cells and tumors marked by each vector to identify the genes targeted by the integrations and elucidate the potential mechanism of deregulation. The described model provide an important tool to compare the risk of insertional mutagenesis of different integrating vectors and provides a platform to test different vector types, designs and safety improvements.
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Dissertations / Theses on the topic "Targeted integration"

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Lacombe, Laurie. "CRISPR-Cas9-based strategies for enhanced targeted integration." Electronic Thesis or Diss., université Paris-Saclay, 2024. http://www.theses.fr/2024UPASL047.

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La transplantation de cellules souches et progénitrices hématopoïétiques (CSPH) corrigées autologues est une stratégie prometteuse pour traiter les troubles génétiques sanguins, immunitaires et métaboliques en raison de leur capacité d'autorenouvellement et de différenciation en cellules sanguines variées. Une des méthodes les plus utilisées pour modifier les CSPH repose sur les outils d'édition de gènes comme les nucléases CRISPR/Cas9. L'intégration ciblée via CRISPR-Cas9 permet l'insertion précise de séquences thérapeutiques, offrant une source durable de cellules corrigées La cassure double brin (CDB) induite par Cas9 peut être réparée par trois voies principales : La jonction d'extrémités non homologues (NHEJ), où les brins libres générés sont ré-ligaturés, ou la recombinaison homologue dirigée (HDR) et la jonction d'extrémités médiée par microhomologie (MMEJ), qui utilise un modèle d'ADN pour la réparation. En fournissant un modèle d'ADN externe, la HDR et la MMEJ peuvent être détournées pour insérer des séquences curatives. Une plateforme de thérapie génique a été développée, avec des locus de refuge comme AAVS1 et HBA. Le locus HBA, en raison de sa forte expression d'α-globine et de sa perte de gène asymptomatique, est particulièrement prometteur pour l'expression de protéines thérapeutiques dans les cellules érythroïdes. Cependant, cette approche prometteuse soulève d'importants défis quant à l'amélioration et à l'évaluation de son efficacité et de sa sécurité.Pour aborder ces défis, divers aspects de l'intégration ciblée basée sur CRISPR/Cas9 ont été étudiés, notamment l'optimisation des méthodes d'administration du modèle ADN, la réduction de la toxicité cellulaire et l'exploitation des voies de réparation pour maximiser l'efficacité de l'intégration.L'un des principaux domaines d'investigation a porté sur la sélection et l'optimisation des méthodes d'administration pour l'introduction de modèles donneurs dans les cellules cibles. Les méthodes d'administration non virales et virales ont été explorées, l'AAV6 et l'IDLV se révélant les plus prometteuses.La toxicité cellulaire, souvent déclenchée par les outils d'intégration ciblée comme l'AAV et CRISPR/Cas9, diminue la viabilité cellulaire en activant la réponse aux dommages de l'ADN, affectant ainsi la capacité de greffe des CSPH. Pour atténuer ces effets génotoxiques, des protocoles visant à réduire la toxicité tout en atteignant des taux suffisants d'intégration ciblée ont été explorés.Les voies de réparation basées sur l'homologie, nécessaires à l'intégration des cassettes, sont limitées à des phases spécifiques du cycle cellulaire. La MMEJ opère pendant les phases G1/S, tandis que la HDR se produit pendant les phases S/G2. La NHEJ, active tout au long du cycle cellulaire, est responsable de la plupart des inconvénients de CRISPR, notamment les aberrations chromosomiques post-CDB.Pour favoriser l'utilisation de la HDR et de la MMEJ, un inhibiteur de la NHEJ a été testé, entraînant une augmentation significative de l'efficacité de l'intégration ciblée avec l'AAV et l'IDLV. Avec l'AAV6 identifié comme méthode optimale d'administration et l'inhibition de la NHEJ comme moyen d'améliorer l'intégration ciblée, la sécurité de la stratégie au niveau du site ciblé a été évaluée. En utilisant le séquençage ciblé à lecture longue, la caractérisation et la quantification des altérations génomiques induites par CRISPR/Cas9 et des événements d'intégration ciblée ont été réalisées, démontrant ainsi l'amélioration de la sécurité de notre approche.Ces résultats mettent en évidence le potentiel de l'inhibition de la NHEJ comme stratégie prometteuse pour améliorer l'édition du génome et l'intégration ciblée de l'ADN
Transplantation of autologous corrected Hematopoietic Stem and Progenitor Cells (HSPCs) emerges as an attractive strategy for treating blood, immune, and metabolic genetic disorders due to their self-renewal capacity and ability to differentiate into any blood cell type. One popular approach to edit HSPCs relies on gene-editing tools such as CRISPR/Cas9 nucleases. Targeted homology-directed integration using CRISPR-Cas9 in HSPCs enables precise insertion of therapeutic sequences, providing a long-term source of corrected cells. The Cas9 induced double-stranded break (DSB) can be repaired via three main pathways: Non-Homologous End Joining (NHEJ), where generated free strands are re-ligated, or Homology Directed Repair (HDR) and Microhomology-mediated End Joining (MMEJ), which uses a template DNA for repair. By providing an external template of interest, MMEJ and HDR can be hijacked to insert curative sequences at a chosen site. A universal gene therapy platform has been developed, with safe harbor loci like AAVS1 and HBA allowing integration of any therapeutic sequence. The HBA locus, with its high α-globin expression and asymptomatic gene loss, is particularly promising allowing the development of a universal platform for expression and secretion of therapeutic proteins into erythroid cells.However, this promising approach raises important challenges about enhancing and evaluating its efficacy and safety. To address these questions, various aspects of CRISPR/Cas9-based targeted integration were investigated, focusing on optimizing delivery methods, minimizing cell toxicity, and exploiting repair pathways to maximize integration efficiency.One of the key areas of investigation centered on the selection and optimization of delivery methods for introducing donor templates into target cells. We explored both non-viral and viral-based delivery approaches.The potential of AAV6 and IDLV delivery methods was found to be the most promising.Cellular toxicity is triggered by numerous tools utilized in targeted integration using CRISPR-Cas9. AAV and CRISPR/Cas9-induced DSBs decrease cell viability by activating the DNA Damage Response, further impacting HSPC engraftment capacity. Concerns about potential genotoxic effects have led us to explore protocols aimed at reducing toxicity while achieving sufficient rates of targeted integration.Homology-based repair pathways necessary for cassette integration are restricted to specific phases of the cell cycle, reducing the window of action for this strategy. MMEJ operates during G1/S phases, while HDR occurs during S/G2 phases. NHEJ, active throughout the cell cycle, is responsible for most CRISPR drawbacks, particularly chromosomal aberrations post-DSB. To promote HDR and MMEJ utilization, a chemical compound known to inhibit NHEJ has been tested. NHEJ inhibition resulted in a significant increase in targeted integration efficiency with both AAV and IDLV-mediated delivery.Having identified AAV6 as the optimal template delivery method and NHEJ inhibition as a means of enhancing targeted integration, the safety of the strategy at the targeted site was assessed. Using targeted long-read sequencing, characterization and quantification of CRISPR/Cas9-induced genomic alterations and targeted integration events were achieved, demonstrating the improved safety of our approach.Overall, these data highlight the potential of NHEJ inhibition as a promising strategy for tuning genome editing and enhancing DNA targeted integration
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Collison, Sean Alfred. "Targeted gene integration for the production of recombinant pharmaceuticals in plants." Thesis, St George's, University of London, 2016. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.706519.

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Production of recombinant protein pharmaceuticals in plants is to become an important biotechnology for improving global health. In addition to having particular advantages for responding to pandemics it also addresses the need for affordable production of pharmaceuticals in developing countries. Whilst the development of transient expression systems has greatly improved the issue of recombinant protein yield from plants, improved expression vectors have also enhanced production from transgenic plants, and there are still drug products for which the technical simplicity of transgenic plant manufacture is important. For transgenic approaches, transgene insertion characteristics such as copy number and sites of insertion are known to affect transgene expression, so the random nature of transgenesis using agrobacterium is problematic, requiring massive screening campaigns to identify optimal lead plant lines. Furthermore, regulatory bodies require detailed characterisation of all genomic alterations resulting from the transformation process. Screening plant lines and genomic and phenotypic analysis is a significant time and cost burden. This thesis describes the design and construction of a series of vectors to develop a targeted gene integration TGI system, utilising the site-specific recombinases PhiC31 integrase and Cre to facilitate predictable gene expression and simplify genomic characterisation by inserting transgenes into predetermined target sites. PhiC31 was applied to perform recombinase- mediated cassette exchange (RMCE), using a promoter-trap system for selection for recombination of the incoming cassette in the correct orientation and allowing the generation of marker-free plants. Cre allowed potential excision of any ectopic integration events occurring during agrobacterium transformation. Over 89 TO target plant lines were generated and screened for recombinant protein production of the fluorescent marker protein, dsRED. They were self-fertilised and 43 T1 progeny were screened by segregation on selective medium. Expression analysis indicated higher transgene expression was associated with single-locus T-DNA insertion. The variation in dsRED expression at TO was also seen in T1 target plant lines, indicating that transgene integration sites are a determining factor for expression yields. Genomic analysis of 22 selected target lines was performed by Southern blot. Single copy T1 target plant lines were transformed with vectors for recombinases and an integration cassette by a novel strategy, to perform RMCE. Putative integrated plant lines were selected for the RMCE event. In a small number of regenerants, loss of dsRED and gain of GUS expression was observed at TO and subsequently in TO backcross plants, indicating successful exchange of the DNA cassettes. This thesis paves the way for a predictable and rapid approach to develop stable transgenic tobacco plants for the production of recombinant pharmaceutical proteins.
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Cortés, Ríos Julio César. "Targeted feedback collection for data source selection with uncertainty." Thesis, University of Manchester, 2018. https://www.research.manchester.ac.uk/portal/en/theses/targeted-feedback-collection-for-data-source-selection-with-uncertainty(3ce078e5-4e5a-4bf5-afb3-1ee51b863925).html.

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The aim of this dissertation is to contribute to research on pay-as-you-go data integration through the proposal of an approach for targeted feedback collection (TFC), which aims to improve the cost-effectiveness of feedback collection, especially when there is uncertainty associated with characteristics of the integration artefacts. In particular, this dissertation focuses on the data source selection task in data integration. It is shown how the impact of uncertainty about the evaluation of the characteristics of the candidate data sources, also known as data criteria, can be reduced, in a cost-effective manner, thereby improving the solutions to the data source selection problem. This dissertation shows how alternative approaches such as active learning and simple heuristics have drawbacks that throw light into the pursuit of better solutions to the problem. This dissertation describes the resulting TFC strategy and reports on its evaluation against alternative techniques. The evaluation scenarios vary from synthetic data sources with a single criterion and reliable feedback to real data sources with multiple criteria and unreliable feedback (such as can be obtained through crowdsourcing). The results confirm that the proposed TFC approach is cost-effective and leads to improved solutions for data source selection by seeking feedback that reduces uncertainty about the data criteria of the candidate data sources.
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Brady, Troy Leon. "The role of the integrase c-terminus in replication and targeted integration of the yeast retrotransposon Ty5." [Ames, Iowa : Iowa State University], 2007.

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Qiao, Junhua [Verfasser], and J. [Akademischer Betreuer] Bode. "Systematic Study on Generation of Mammalian Production Cell Lines by Targeted Integration (RMCE) / Junhua Qiao ; Betreuer: J. Bode." Braunschweig : Technische Universität Braunschweig, 2009. http://d-nb.info/1175828807/34.

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Hurlburt, Michael S. "An empirical framework for the evaluation of mental health care strategies targeted to community integration of severely mentally ill homeless individuals /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC IP addresses, 1997. http://wwwlib.umi.com/cr/ucsd/fullcit?p9722820.

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Hassan, Aamir Ul. "Integration of Genome Scale Data for Identifying New Biomarkers in Colon Cancer: Integrated Analysis of Transcriptomics and Epigenomics Data from High Throughput Technologies in Order to Identifying New Biomarkers Genes for Personalised Targeted Therapies for Patients Suffering from Colon Cancer." Thesis, University of Bradford, 2017. http://hdl.handle.net/10454/17419.

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Colorectal cancer is the third most common cancer and the leading cause of cancer deaths in Western industrialised countries. Despite recent advances in the screening, diagnosis, and treatment of colorectal cancer, an estimated 608,000 people die every year due to colon cancer. Our current knowledge of colorectal carcinogenesis indicates a multifactorial and multi-step process that involves various genetic alterations and several biological pathways. The identification of molecular markers with early diagnostic and precise clinical outcome in colon cancer is a challenging task because of tumour heterogeneity. This Ph.D.-thesis presents the molecular and cellular mechanisms leading to colorectal cancer. A systematical review of the literature is conducted on Microarray Gene expression profiling, gene ontology enrichment analysis, microRNA and system Biology and various bioinformatics tools. We aimed this study to stratify a colon tumour into molecular distinct subtypes, identification of novel diagnostic targets and prediction of reliable prognostic signatures for clinical practice using microarray expression datasets. We performed an integrated analysis of gene expression data based on genetic, epigenetic and extensive clinical information using unsupervised learning, correlation and functional network analysis. As results, we identified 267-gene and 124-gene signatures that can distinguish normal, primary and metastatic tissues, and also involved in important regulatory functions such as immune-response, lipid metabolism and peroxisome proliferator-activated receptors (PPARs) signalling pathways. For the first time, we also identify miRNAs that can differentiate between primary colon from metastatic and a prognostic signature of grade and stage levels, which can be a major contributor to complex transcriptional phenotypes in a colon tumour.
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LLADO, SANTAEULARIA MANEL. "THERAPEUTIC GENOME EDITING IN RETINA AND LIVER." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/696628.

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In vivo gene therapy with adeno-associated viral (AAV) vectors has been successful at treating several inherited diseases, specifically those caused by loss of function mutations which require transfer of a correct copy of a gene. This would not benefit dominant diseases due to gain of function mutations which produce toxic protein products. In addition, since AAV genomes persist as episomes in target cells, AAV mediated transgene expression might be short lived in tissues where cell proliferation occurs when newborn or after damage, like for example the liver. To overcome these challenges, I have developed AAV-based therapeutic approaches which use genome editing to introduce stable modifications at specific genomic loci. First, an allele-specific approach which targets the Rhodopsin P347S dominant mutation was developed and tested both in vitro and in vivo. I achieved allele-specific targeting of human P347S rhodopsin, which reduced mRNA levels and improved retinal electrical function in a mouse model of autosomal dominant retinitis pigmentosa. Second, I developed a mutation- and homology-independent targeted integration (HITI) approach for gene correction in photoreceptors. I demonstrated feasibility of this approach in mouse and pig photoreceptors using a reporter gene and characterized on-target precision of HITI in the murine rhodopsin locus. I then tested the therapeutic potential of this approach in a mouse model of autosomal dominant retinitis pigmentosa and observed mild and transient improvement of retinal function in treated eyes, which suggests that the levels of editing obtained need optimization. Third, I developed a HITI approach for expressing therapeutic genes from the liver by targeting the albumin locus, which is highly transcribed in hepatocytes. I demonstrated feasibility and efficiency of this approach using a reporter gene, and characterized on-target precision of HITI, as well as off-target integration due to Cas9 cleavage. I then tested the therapeutic potential of the integration of a copy of the human arylsulfatase B (ARSB) gene, which is mutated in a rare lysosomal storage disease, mucopolysaccharidosis type VI (MPS VI), in the albumin locus in the liver of newborn MPSVI mice. I demonstrated that this approach achieves stable expression of ARSB at levels that reduce glucosaminoglycan (GAG) urinary secretion, one of the main readouts of MPSVI phenotype. This stable expression of ARSB is contrary to the decrease of transgene expression observed in neonatal MPSVI mice injected with the same dose of a conventional gene therapy vector, thus overcoming the potential loss of transgene expression caused by hepatocyte proliferation. Overall, I have developed different genome editing approaches for conditions that are inherited as either dominant or recessive. I have tested these approaches in two relevant tissues for gene therapy like retina and liver and shown the potential to provide AAV with persistent transgene expression in proliferating tissues like the newborn liver.
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Tirovolis, Nikolaos Labros. "Integration of commercial aircraft economic targets into the initial design process." Thesis, Imperial College London, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.425793.

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Ewart, Steven. "Starting hand position effects on arm configuration for targeted reaching movements." Thesis, University of Iowa, 2014. https://ir.uiowa.edu/etd/4625.

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Books on the topic "Targeted integration"

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Bank, World, ed. Clearing the global health fog: A systematic review of the evidence on integration of health systems and targeted interventions. Washington, D.C: World Bank, 2009.

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Normand, Andrew C. Proposals for the integration of aims, objectives and targets in the Scottish criminial justice system. Edinburgh: Scottish Executive, 2003.

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Africa Institute of South Africa, ed. Millennium development goals: Achievements and prospects of meeting the targets in Africa. Pretoria, South Africa: Africa Institute of South Africa, 2008.

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Benucci, Antonella, Giulia I. Grosso, and Viola Monaci. Linguistica Educativa e contesti migratori. Venice: Fondazione Università Ca’ Foscari, 2021. http://dx.doi.org/10.30687/978-88-6969-570-4.

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The volume, produced within the framework of the COMMIT project “Fostering the Integration of Resettled Refugees in Croatia, Italy, Portugal and Spain”, concerns the current European situation, and in particular the teaching of L2 in its relations and interdisciplinary exchanges with other scientific fields dealing with migratory phenomena; therefore, starting from the COMMIT experience, it offers a wide perspective, going beyond the borders of the countries involved in the project and identifying good practices that can be replicated in different territorial and social contexts to ensure successful social inclusion of newly arrived citizens. COMMIT is a project funded by the European Commission (DG HOME), co-financed by the Ministry of Interior and the Project Partners and managed by the Mediterranean Coordination Office of the International Organization for Migration (IOM), in Italy. The project was implemented in collaboration with the IOM Missions in Croatia, Portugal and Spain, together with the Communitas Consortium, the Adecco Foundation for Equal Opportunities and the University for Foreigners of Siena (UNISTRASI). The project activities were implemented from 1 January 2019 to 30 April 2021. The project, based on the idea that successful integration of resettled refugees occurs both by putting in place certain structural conditions and by promoting mutual exchange between resettled refugees and their host communities, aimed to support their integration into their new communities, with a special focus on women and young refugees as particularly vulnerable groups. A secure humanitarian migration route to the European Union launched in 2013 is targeted at refugees who are beneficiaries of resettlement. Several Member States, including Croatia, Italy, Portugal and Spain, have therefore established or strengthened their national resettlement and humanitarian admission programmes for resettled refugees of Syrian, Eritrean, Ethiopian or Sudanese origin. In preparation for resettlement, beneficiaries participate in a series of pre-departure cultural orientation activities. Among them, training in L2 language and culture plays a crucial role. The book hence tries to offer answers to the many challenges that characterise the field of language education in contexts marked by the presence of migrants from an interdisciplinary perspective. It provides for effective solutions for an inclusive language education, attentive to ‘vulnerable’ subjects, paying attention to the interweaving of complex individual, social, cultural and economic contexts, such as school and university training courses and reception and resettlement programmes in host societies. In particular, the current situation in Italy, regarding both teaching L2 in a school context and teaching modern languages to adult foreigners, is still lacking in interdisciplinary relations and exchanges between language teaching and other scientific fields dealing with migratory phenomena. However, in recent years a particular sensitivity and empathy towards linguistic and cultural contact have developed.
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V, Kuosmanen, and Gaál Gabor, eds. Exploration target selection by integration of geodata using statistical and image processing techniques, an example from central Finland. Espoo: Geological Survey of Finland, 1988.

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McKay, C. A study of system interface sets (SIS) for the host, target and integration environments of the space station program (SSP). [Houston, Tex.]: Research Institute for Computing and Information Systems, University of Houston-Clear Lake, 1987.

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Combelles-Siegel, Pascale. Target Bosnia: Integrating information activities in peace operations : NATO-led operations in Bosnia-Herzegovina, December 1995-1997. Washington, DC: National Defense University, 1998.

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Levesque, Roger J. R. The Dramatic Clarification of School Segregation and Diversity Law. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780190633639.003.0001.

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Educational segregation is spreading. Although rising percentages of different ethnic/racial and multiracial groups complicate statistical findings, Blacks still remain substantially more segregated from Whites than other racial/ethnic groups, even despite the legacy of school desegregation and integration efforts targeted at Black youth. This chapter examines the spread of educational segregation and highlights how the legal system increasingly views such segregation as outside of the legal system’s reach. It examines how the legal system approaches discrimination and notes how the book explores the source, nature, and implications of what constitutes segregation and related phenomena, such as desegregation, integration, and discrimination. It concludes by detailing the arguments made in the remainder of the book.
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Kaufmann, Philipp A., and Oliver Gaemperli. Hybrid Cardiac Imaging. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199392094.003.0028.

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Assessment of both coronary anatomy and myocardial perfusion are equally important for the appropriate treatment of patients with stable coronary artery disease. Cardiac hybrid imaging allows integration of coronary anatomy and perfusion in one all-in-one image, thereby avoiding mental integration of findings. In selected subgroups of patients, cardiac hybrid imaging has demonstrated superior diagnostic accuracy compared to single modalities. The combination of coronary anatomy and function provides incremental prognostic information and improves risk stratification of patients with suspected or known CAD. Aside from CT coronary angiography, coronary artery calcium score (CACS) scans obtained from native ECG-triggered CT are used for hybrid imaging. They are used either for attenuation correction, or can be combined with radionuclide information to improve CAD detection and risk stratification. A large number of integrated hybrid scanners are commercially available and offer advantages for cardiac hybrid imaging. However, these devices are not mandatory, and hybrid imaging is perfectly feasible from two separate datasets using appropriate image fusion software. Cardiac magnetic resonance has entered the arena of hybrid imaging and several integrated PET/MRI devices are already commercially available. Its advantages include the lack of ionizing radiation and a high spatial resolution, particularly for soft tissue structures. In research, hybrid imaging moves beyond its conventional borders of perfusion imaging to target specific molecular or biological pathways that underlie cardiac disease, a concept known as molecular imaging. The combination of radionuclide imaging with CT or MRI offers attractive features to co-localize biological signals from radiolabeled targeted compounds with microanatomical structures.
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Burton, Derek, and Margaret Burton. Integration and control: hormones. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780198785552.003.0010.

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Fish, and other vertebrates, possess both a neural control system and a hormonal one dependent on the circulation. Fish hormones may be secreted by well-defined endocrine glands or by diffuse tissue and can have profound effects on the function of target tissues or organs which possess receptor molecules recognized by specific hormones. Some endocrine activities in fish are not relevant to mammals, and vice versa. Thus fish can have chromatophores controlled hormonally to change skin colour, the same hormones having different roles in mammals. Likewise, ‘prolactin’ in fish cannot regulate lactation. Individual endocrine glands, tissues and cells of fish are described in detail and the possible roles of their hormones are discussed. Fish endocrinology is becoming increasingly important with the realization of its potential applications in aquaculture and with the recognition of the consequences of pollutant endocrine disruption.
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Book chapters on the topic "Targeted integration"

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Jones, Kathryn S., Joseph Kulkosky, and Anna Marie Skalka. "Analyses of HIV Integration Components." In Advances in Molecular Biology and Targeted Treatment for AIDS, 21–26. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5928-9_2.

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Tsakraklides, Vasiliki. "Targeted Integration Through Transformation of Hydroxyurea-Arrested Cells." In Methods in Molecular Biology, 139–45. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1414-3_9.

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Gama-Norton, L., P. Riemer, U. Sandhu, K. Nehlsen, R. Schucht, H. Hauser, and D. Wirth. "Defeating Randomness – Targeted Integration as a Boost for Biotechnology." In Cell Engineering, 53–82. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-2245-5_3.

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Sherman, Paula A., and James A. Fyfe. "DNA Cleaving Activity of Purified Human Immunodeficiency Virus Integration Protein." In Advances in Molecular Biology and Targeted Treatment for AIDS, 27–33. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5928-9_3.

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Schenkwein, Diana, and Seppo Ylä-Herttuala. "Development of Lentiviral Vectors for Targeted Integration and Protein Delivery." In Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools, 185–98. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3753-0_14.

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Gersbach, Charles A., and Carlos F. Barbas. "Targeted Plasmid Integration into the Human Genome by Engineered Recombinases." In Site-directed insertion of transgenes, 267–84. Dordrecht: Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-94-007-4531-5_10.

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Malmqvist, Lars. "Rapid Synergy Realisation in Post-Merger Integration Through Targeted Transition Architectures." In Advances in Information and Communication Technology, 432–39. Cham: Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-50818-9_46.

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Biggs, Daniel, Chiann-mun Chen, and Benjamin Davies. "Targeted Integration of Transgenes at the Mouse Gt(ROSA)26Sor Locus." In Methods in Molecular Biology, 299–323. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2990-1_13.

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Chiorini, J. A., S. M. Wiener, L. Yang, R. H. Smith, B. Safer, N. P. Kilcoin, Y. Liu, E. Urcelay, and R. M. Kotin. "The Roles of AAV Rep Proteins in Gene Expression and Targeted Integration." In Adeno-Associated Virus (AAV) Vectors in Gene Therapy, 25–33. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-80207-2_2.

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Fluthgraf, Sandra, Barbara Schruff, and Ulrich Klinner. "ADH1 Disruption in Pichia stipitis by Targeted Restriction Enzyme Mediated Integration (tREMI)." In Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology, 221–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-55758-3_35.

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Conference papers on the topic "Targeted integration"

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Mayer, Ingrid A. "Abstract ES3-3: Integration of novel targeted agents with endocrine therapy." In Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium; December 9-13, 2014; San Antonio, TX. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.sabcs14-es3-3.

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Ferguson, Scott, CallawayTurner Turner, and Joseph Donndelinger. "Targeted Initial Populations for Multiobjective Product Line Optimization." In 12th AIAA Aviation Technology, Integration, and Operations (ATIO) Conference and 14th AIAA/ISSMO Multidisciplinary Analysis and Optimization Conference. Reston, Virigina: American Institute of Aeronautics and Astronautics, 2012. http://dx.doi.org/10.2514/6.2012-5443.

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Kubota, Tetsushi, Shinji Kuroda, Toshiaki Morihiro, Hiroshi Tazawa, Shunsuke Kagawa, and Toshiyoshi Fujiwara. "Abstract 4747: Novel HER2-targeted gold nanoparticles; integration of antibody therapy and nanotechnology." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-4747.

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El-Sayed, Mohamed, David Lingerfelt, Austin McGuire, and Allan Wicker. "Design and Development of a Folding Electrically Assisted Bicycle." In ASME 2016 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/imece2016-66238.

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With the rapid development of hybrid and electrically driven transportation systems, several concepts of electrically driven bicycles have been developed and introduced to the market. Also, for storage advantage and portability several versions of folding bikes are available in the market. In this paper, the design, development and validation process for creating a version of a folding electrically assisted bicycle is presented. The designed, developed, and road tested bicycle concept is targeted for use by college students, exercise enthusiast, or for commuting to work. It is also intended as a means of transportation for those living in large cities with a considerable riding distance and a limited space to store a bicycle. In addition to being foldable, the electrically assisted bicycle is designed with a small footprint and a ride performance comparable to any normal city bicycle. To achieve the design objectives, the process starts with identifying the key attributes such as portability, durability, drivability, maintainability, and safety. The steps for translating the targeted attributes to design criteria and product specifications are discussed. Consequently, the analysis and integration technical tasks needed to achieve the established bicycle specifications for both architectural and performance integration efforts are identified. During the early stages of the design phase, different alternatives and off-the-shelf components are considered. Architectural and performance integration activities including detailed virtual modeling, simulations, and analyses are implemented to develop the bicycle and achieve targeted attributes within the design constraints. For concept proofing, the designed concept was developed and road tested. Preliminary results of physical testing demonstrate the achievement levels of different targeted attributes. While some targets were achieved, the initial physical tests indicate that further design improvements are needed through additional development and validation iterations. These improvements could be achieved through adjustment of targets, weight reduction, and alternative materials.
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Verma, Dinesh, Greg Cirincione, Tien Pham, and Bong Jun Ko. "Generation and management of training data for AI-based algorithms targeted at coalition operations." In Ground/Air Multisensor Interoperability, Integration, and Networking for Persistent ISR IX, edited by Tien Pham, Michael A. Kolodny, and Dietrich M. Wiegmann. SPIE, 2018. http://dx.doi.org/10.1117/12.2305244.

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Hsieh, Wen-Fei, Shih-Hsiang Tseng, and Bo Min She. "The Novel TEM Sample Preparation Approach for Targeted via with Barrier/Cu Seed Layer Inspection." In ISTFA 2009. ASM International, 2009. http://dx.doi.org/10.31399/asm.cp.istfa2009p0214.

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Abstract In this study, an FIB-based cross section TEM sample preparation procedure for targeted via with barrier/Cu seed layer is introduced. The dual beam FIB with electron beam for target location and Ga ion beam for sample milling is the main tool for the targeted via with barrier/Cu seed layer inspection. With the help of the FIB operation and epoxy layer protection, ta cross section TEM sample at a targeted via with barrier/Cu seed layer could be made. Subsequent TEM inspection is used to verify the quality of the structure. This approach was used in the Cu process integration performance monitor. All these TEM results are very helpful in process development and yield improvement.
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Radzikowski, Kacper, Osamu Yoshie, and Robert Nowak. "Support software for Automatic Speech Recognition systems targeted for non-native speech." In iiWAS '20: The 22nd International Conference on Information Integration and Web-based Applications & Services. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3428757.3429971.

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Zhuang, Huiying, Sandip Parikh, and Sandeep Shah. "Targeted Boiler Cleaning Using Smart Sootblower." In ASME 2006 Power Conference. ASMEDC, 2006. http://dx.doi.org/10.1115/power2006-88075.

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A Smart Sootblower has been developed to meet the increasing demand for advanced boiler cleaning equipment. Utility plants are challenged to burn coals with severe slagging tendency. Slagging condition may be significantly different from pendant to pendant across the boiler. Conventional retractable sootblowers cleans superheat and reheat pendants with even intensity, resulting in over cleaning of some pendants and “clinker” buildup in others. Modern instrumentation such as SuperHeat Fouling Monitoring (SHFM) systems and Video Cameras is able to locate the exact spot where the slag is accumulated. With the help of these detention systems, the Smart Sootblower, a multi-mode soot blower, is able to target and adjust clean intensity based on slagging condition, thus minimizing tube leakage and clinker formation. The Smart Sootblower has two motors that independently control translation and rotation motion. This allows the sootblower to change helix for different cleaning in different sections of the boiler. Operators can select zones require aggressive cleaning and zones need less or no cleaning. Integration of SuperHeat Fouling Monitoring (SHFM) system would provide operators the knowledge of fouling condition and enable automatic control of sootblowers for optimal performance. Actual installations and data are presented.
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McNeely, Marcus. "Leveraging Integration of Calibration Management Systems in Healthcare Metrology." In NCSL International Workshop & Symposium. NCSL International, 2015. http://dx.doi.org/10.51843/wsproceedings.2015.20.

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Software packages with targeted calibration and maintenance functionality have long been incorporated to track calibration, maintenance and asset management for IM&TE in regulated environments with an ever-increasing list of functionality to meet specific needs of the end-user group. As corporations move towards implementations of major ERP (Enterprise Resource Planning) software applications, resident calibration functionality ´modules’ are increasingly mandated to metrology organizations, often without user acceptance, with corporate focus on efficiency of implementing a single solution at the cost functionality that falls short of specific metrology user requirements. API (Application Programming Interface, AKA, Integration) is a viable option to leverage the desired functionality between two or more systems’ data. Integration is a user-defined interaction (to the field-level) so that key information can be ´pushed’ or ´pulled’ to and from target and source software packages. This paper discusses the strategy of justifying and incorporating integration between an ERP (or other software systems) and desired CMMS software (Calibration/Maintenance Management System), its architecture overview and common validation plans to satisfy corporate quality and IT organizations in order to maximize compliance and efficiency in the metrology department
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Escudeiro, Paula, Márcia Campos, Francisca Escudeiro, and Nuno Escudeiro. "A serious game for the cognitive stimulation of seniors." In Intelligent Human Systems Integration (IHSI 2024) Integrating People and Intelligent Systems. AHFE International, 2024. http://dx.doi.org/10.54941/ahfe1004479.

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With the increasing number of elderly individuals in the Portuguese population and the consequent rise in cognitive decline associated with normal aging, there is a growing need to invest in mental health. This investment can be realized through the promotion of active aging and cognitive stimulation. Developing interactive tools that provide stimulation and motivation for everyday activities proves to be an effective strategy in slowing down cognitive decline. These tools aim to simulate familiar everyday scenarios for end-users, thereby encouraging regular use. This project focuses on the design and implementation of a platform to assist in evaluating and training the cognitive capacities of adults. The platform facilitates early awareness of cognitive deficiencies and stimulates users with pre-clinical symptoms, eliminating the need for visits to a medical office. It presents an innovative alternative to traditional tests conducted in clinical environments. Specifically, the platform recreates scenarios encountered during appointments with psychologists, transforming them into a game consisting of minigames. These minigames mimic real-world tasks, enabling a seamless integration of users' daily life results and enhancing their interaction with the environment. This approach aims to prevent the stress often associated with traditional cognitive stimulation programs and yield more accurate results without the pressure of a clinical setting. The primary goal is to develop a serious game that efficiently links simulation results to the daily activities of the targeted audience. This game adapts a scientifically validated cognitive training program to an Information Technology (IT) platform. The most significant outcome of this work is the cognitive stimulation of users and the effective integration of stimulation results. Through this serious game, the project strives to promote active aging, cognitive stimulation, and mental health using an interactive platform tailored to individual everyday life and activities.
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Reports on the topic "Targeted integration"

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Halas, Naomi J. Seamless Integration of Detection and Therapy for Breast Cancer Using Targeted Engineered Nanoparticles. Fort Belvoir, VA: Defense Technical Information Center, June 2005. http://dx.doi.org/10.21236/ada446343.

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Halas, Naomi J. Seamless Integration of Detection and Therapy for Breast Cancer using Targeted Engineered Nanoparticles. Fort Belvoir, VA: Defense Technical Information Center, June 2006. http://dx.doi.org/10.21236/ada465826.

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Halas, Naomi J. Seamless Integration of Detection and Therapy for Breast Cancer using Targeted Engineered Nanoparticles. Fort Belvoir, VA: Defense Technical Information Center, June 2007. http://dx.doi.org/10.21236/ada473391.

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Volpe Martincus, Christian, Mauricio Mesquita Moreira, Kati Suominen, Rosario Campos, Álvaro Inchauspe, Luis Torres Paz, Elena Achar Samra, et al. Integration & Trade Journal: Volume 17: No. 37: July-December, 2013. Inter-American Development Bank, January 2014. http://dx.doi.org/10.18235/0008280.

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In spite of the fact that they make up more than 90% of firms and account for a substantial portion of employment in these countries, rather little is known about the role of small- and mid-size enterprises (SMEs) in Latin American and the Caribbean (LAC) trade. In particular, there are a series of pending yet pivotal questions, such as: To what extent and how do SMEs contribute to their countries exports? How internationalized are LAC SMEs compared to SMEs in other world regions? How diversified are LAC SMEs exports across products and destinations? What are the key bottlenecks to LAC SME internationalization? This issue of the Journal intends to drive a holistic, thoughtful and targeted discussion of patterns of SME internationalization and its major bottlenecks and how they can be (or not) mitigated most effectively.
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Röders, Jonathan, and Mari Mirasol. Research Brief: Deconstructing the Formerly Armed Actor Threat Stigma. Trust After Betrayal, January 2023. http://dx.doi.org/10.59498/68050.

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This research brief highlights the critical importance of deconstructing the threat stigma associated with formerly armed actors (FAAs) to ensure a more inclusive and successful (re)integration process. By challenging the prevailing perception that FAAs are inherently violent beings, disarmament, demobilisation, and reintegration (DDR) programmes can promote societal acceptance and prevent the marginalisation of FAAs. Public awareness campaigns that contribute to a more nuanced understanding of their backgrounds play a key role in reshaping community perceptions and empowering FAAs as active agents in the (re)integration process. Recognising them as individuals with complex motives and experiences, avoiding stigmatising language and providing targeted support and opportunities for their social reintegration, enhance their chances of becoming valued and productive members of their communities.
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Tzfira, Tzvi, Michael Elbaum, and Sharon Wolf. DNA transfer by Agrobacterium: a cooperative interaction of ssDNA, virulence proteins, and plant host factors. United States Department of Agriculture, December 2005. http://dx.doi.org/10.32747/2005.7695881.bard.

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Agrobacteriumtumefaciensmediates genetic transformation of plants. The possibility of exchanging the natural genes for other DNA has led to Agrobacterium’s emergence as the primary vector for genetic modification of plants. The similarity among eukaryotic mechanisms of nuclear import also suggests use of its active elements as media for non-viral genetic therapy in animals. These considerations motivate the present study of the process that carries DNA of bacterial origin into the host nucleus. The infective pathway of Agrobacterium involves excision of a single-stranded DNA molecule (T-strand) from the bacterial tumor-inducing plasmid. This transferred DNA (T-DNA) travels to the host cell cytoplasm along with two virulence proteins, VirD2 and VirE2, through a specific bacteriumplant channel(s). Little is known about the precise structure and composition of the resulting complex within the host cell and even less is known about the mechanism of its nuclear import and integration into the host cell genome. In the present proposal we combined the expertise of the US and Israeli labs and revealed many of the biophysical and biological properties of the genetic transformation process, thus enhancing our understanding of the processes leading to nuclear import and integration of the Agrobacterium T-DNA. Specifically, we sought to: I. Elucidate the interaction of the T-strand with its chaperones. II. Analyzing the three-dimensional structure of the T-complex and its chaperones in vitro. III. Analyze kinetics of T-complex formation and T-complex nuclear import. During the past three years we accomplished our goals and made the following major discoveries: (1) Resolved the VirE2-ssDNA three-dimensional structure. (2) Characterized VirE2-ssDNA assembly and aggregation, along with regulation by VirE1. (3) Studied VirE2-ssDNA nuclear import by electron tomography. (4) Showed that T-DNA integrates via double-stranded (ds) intermediates. (5) Identified that Arabidopsis Ku80 interacts with dsT-DNA intermediates and is essential for T-DNA integration. (6) Found a role of targeted proteolysis in T-DNA uncoating. Our research provide significant physical, molecular, and structural insights into the Tcomplex structure and composition, the effect of host receptors on its nuclear import, the mechanism of T-DNA nuclear import, proteolysis and integration in host cells. Understanding the mechanical and molecular basis for T-DNA nuclear import and integration is an essential key for the development of new strategies for genetic transformation of recalcitrant plant species. Thus, the knowledge gained in this study can potentially be applied to enhance the transformation process by interfering with key steps of the transformation process (i.e. nuclear import, proteolysis and integration). Finally, in addition to the study of Agrobacterium-host interaction, our research also revealed some fundamental insights into basic cellular mechanisms of nuclear import, targeted proteolysis, protein-DNA interactions and DNA repair.
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Wilson, Thomas E., Avraham A. Levy, and Tzvi Tzfira. Controlling Early Stages of DNA Repair for Gene-targeting Enhancement in Plants. United States Department of Agriculture, March 2012. http://dx.doi.org/10.32747/2012.7697124.bard.

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Gene targeting (GT) is a much needed technology as a tool for plant research and for the precise engineering of crop species. Recent advances in this field have shown that the presence of a DNA double-strand break (DSB) in a genomic locus is critical for the integration of an exogenous DNA molecule introduced into this locus. This integration can occur via either non-homologous end joining (NHEJ) into the break or homologous recombination (HR) between the broken genomic DNA and the introduced vector. A bottleneck for DNA integration via HR is the machinery responsible for homology search and strand invasion. Important proteins in this pathway are Rad51, Rad52 and Rad54. We proposed to combine our respective expertise: on the US side, in the design of zincfinger nucleases (ZFNs) for the induction of DNA DSBs at any desired genomic locus and in the integration of DNA molecules via NHEJ; and on the Israeli side in the HR events, downstream of the DSB, that lead to homology search and strand invasion. We sought to test three major pathways of targeted DNA integration: (i) integration by NHEJ into DSBs induced at desired sites by specially designed ZFNs; (ii) integration into DSBs induced at desired sites combined with the use of Rad51, Rad52 and Rad54 proteins to maximize the chances for efficient and precise HR-mediated vector insertion; (iii) stimulation of HR by Rad51, Rad52 and Rad54 in the absence of DSB induction. We also proposed to study the formation of dsT-DNA molecules during the transformation of plant cells. dsT-DNA molecules are an important substrate for HR and NHEJ-mediatedGT, yet the mode of their formation from single stranded T-DNA molecules is still obscure. In addition we sought to develop a system for assembly of multi-transgene binary vectors by using ZFNs. The latter may facilitate the production of binary vectors that may be ready for genome editing in transgenic plants. ZFNs were proposed for the induction of DSBs in genomic targets, namely, the FtsH2 gene whose loss of function can easily be identified in somatic tissues as white sectors, and the Cruciferin locus whose targeting by a GFP or RFP reporter vectors can give rise to fluorescent seeds. ZFNs were also proposed for the induction of DSBs in artificial targets and for assembly of multi-gene vectors. We finally sought to address two important cell types in terms of relevance to plant transformation, namely GT of germinal (egg) cells by floral dipping, and GT in somatic cells by root and leave transformation. To be successful, we made use of novel optimized expression cassettes that enable coexpression of all of the genes of interest (ZFNs and Rad genes) in the right tissues (egg or root cells) at the right time, namely when the GT vector is delivered into the cells. Methods were proposed for investigating the complementation of T-strands to dsDNA molecules in living plant cells. During the course of this research, we (i) designed, assembled and tested, in vitro, a pair of new ZFNs capable of targeting the Cruciferin gene, (ii) produced transgenic plants which expresses for ZFN monomers for targeting of the FtsH2 gene. Expression of these enzymes is controlled by constitutive or heat shock induced promoters, (iii) produced a large population of transgenic Arabidopsis lines in which mutated mGUS gene was incorporated into different genomic locations, (iv) designed a system for egg-cell-specific expression of ZFNs and RAD genes and initiate GT experiments, (v) demonstrated that we can achieve NHEJ-mediated gene replacement in plant cells (vi) developed a system for ZFN and homing endonuclease-mediated assembly of multigene plant transformation vectors and (vii) explored the mechanism of dsTDNA formation in plant cells. This work has substantially advanced our understanding of the mechanisms of DNA integration into plants and furthered the development of important new tools for GT in plants.
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Dors, Nathan. WebISO: Target-Side Integration Models. Internet2, October 2003. http://dx.doi.org/10.26869/ti.17.1.

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Urban, Peter, and Eva Peschke. Multibeam echo grid simulator - Short description of the research software. GEOMAR Helmholtz Centre for Ocean Research Kiel, Germany, 2023. http://dx.doi.org/10.3289/sw_3_2023.

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This repository contains the simulation code (python) for reproducing the results of the accepted paper "Echo grid integration: A novel method for preprocessing multibeam water column data to quantify underwater gas bubble emissions." by Urban et. al. 2023 (Accepted) The program simulates multibeam water column images for virtual surveys over individual targets or target clouds and allows for testing the effects of different echo grid integration methods. (See Urban et. al 2023 for more details)
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Puestow. L52194 Detection of Third Party Encroachment Using Satellite Based Remote Sensing Technologies. Chantilly, Virginia: Pipeline Research Council International, Inc. (PRCI), July 2015. http://dx.doi.org/10.55274/r0011045.

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Building on past experience, it was the objective of this investigation to automate the satellite-based detection of encroachment events, to improve target detection and reduce false alarms using radar and optical imagery and to investigate the integration of one-call services into the process flow. Algorithm development for target detection using optical imagery was carried out with the intention to facilitate the future integration of unmanned airborne vehicle (UAV) technology into the process. The capacity of the multitemporal algorithm was extended to enable the detection of area changes in addition to vehicle targets. The integration of existing notification services in the satellite-based approach was examined. A satellite-based encroachment monitoring system is now in place to undertake large-scale field demonstrations over 100 to 200 miles of right-of-way for a period of several months, preceded by a pre-service calibration phase of several weeks to adjust the procedures to local conditions. A constant false alarm rate between 5 and 10% can be achieved after a service period of 8 to 10 months.
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