Relevant bibliographies by topics / Tandem mass spectrometry

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Tandem mass spectrometry'

Author: Grafiati
Published: 4 June 2021
Last updated: 19 October 2024

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Journal articles on the topic "Tandem mass spectrometry"

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Glish, Gary L., and David J. Burinsky. "Hybrid mass spectrometers for tandem mass spectrometry." Journal of the American Society for Mass Spectrometry 19, no. 2 (February 2008): 161–72. http://dx.doi.org/10.1016/j.jasms.2007.11.013.

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Kumar, Praveen. "Tandem Mass Spectrometry." Journal of Neonatology 19, no. 2 (June 2005): 180–83. http://dx.doi.org/10.1177/0973217920050214.

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Busch, Kenneth L., Gary L. Glish, Scott A. McLuckey, and John J. Monaghan. "Mass spectrometry/mass spectrometry: techniques and applications of tandem mass spectrometry." Analytica Chimica Acta 237 (1990): 509. http://dx.doi.org/10.1016/s0003-2670(00)83956-2.

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Futrell, Jean H. "Mass spectrometry/mass spectrometry: Techniques and applications of tandem mass spectrometry." Microchemical Journal 41, no. 2 (April 1990): 246–47. http://dx.doi.org/10.1016/0026-265x(90)90124-n.

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Moriarty, F. "Mass spectrometry/mass spectrometry. Techniques and applications of tandem mass spectrometry." Environmental Pollution 61, no. 3 (1989): 261. http://dx.doi.org/10.1016/0269-7491(89)90246-7.

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Cooks, R. G. "Mass Spectrometry/Mass Spectrometry. Techniques and Applications of Tandem Mass Spectrometry." International Journal of Mass Spectrometry and Ion Processes 93, no. 2 (October 1989): 265–66. http://dx.doi.org/10.1016/0168-1176(89)80103-x.

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Tian, Qingguo, and Steven J. Schwartz. "Mass Spectrometry and Tandem Mass Spectrometry of Citrus Limonoids." Analytical Chemistry 75, no. 20 (October 2003): 5451–60. http://dx.doi.org/10.1021/ac030115w.

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Shoji, Yuki, Mari Yotsu-Yamashita, Teruo Miyazawa, and Takeshi Yasumoto. "Electrospray Ionization Mass Spectrometry of Tetrodotoxin and Its Analogs: Liquid Chromatography/Mass Spectrometry, Tandem Mass Spectrometry, and Liquid Chromatography/Tandem Mass Spectrometry." Analytical Biochemistry 290, no. 1 (March 2001): 10–17. http://dx.doi.org/10.1006/abio.2000.4953.

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Budzikiewicz, H. "Selected reviews on mass spectrometric topics. XXVIII. Tandem mass spectrometry." Mass Spectrometry Reviews 8, no. 2 (March 1989): 119. http://dx.doi.org/10.1002/mas.1280080204.

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Pisitkun, Trairak, Jason D. Hoffert, Ming-Jiun Yu, and Mark A. Knepper. "Tandem Mass Spectrometry in Physiology." Physiology 22, no. 6 (December 2007): 390–400. http://dx.doi.org/10.1152/physiol.00025.2007.

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Tandem mass spectrometry coupled to liquid chromatography (LC-MS/MS) allows identification of proteins in a complex mixture without need for protein purification (“shotgun” proteomics). Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology.
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Dissertations / Theses on the topic "Tandem mass spectrometry"

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Offei, Felix. "Denoising Tandem Mass Spectrometry Data." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etd/3218.

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Protein identification using tandem mass spectrometry (MS/MS) has proven to be an effective way to identify proteins in a biological sample. An observed spectrum is constructed from the data produced by the tandem mass spectrometer. A protein can be identified if the observed spectrum aligns with the theoretical spectrum. However, data generated by the tandem mass spectrometer are affected by errors thus making protein identification challenging in the field of proteomics. Some of these errors include wrong calibration of the instrument, instrument distortion and noise. In this thesis, we present a pre-processing method, which focuses on the removal of noisy data with the hope of aiding in better identification of proteins. We employ the method of binning to reduce the number of noise peaks in the data without sacrificing the alignment of the observed spectrum with the theoretical spectrum. In some cases, the alignment of the two spectra improved.
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Dabney, David E. "Analysis of Synthetic Polymers by Mass Spectrometry and Tandem Mass Spectrometry." University of Akron / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=akron1259021862.

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Jackson, Anthony Trevor. "Tandem mass spectrometry of polymeric materials." Thesis, University of Warwick, 1995. http://wrap.warwick.ac.uk/44296/.

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Mass spectrometry and tandem mass spectrometry (MS/MS) has been employed to analyse peptides (<600 daltons), synthetic polymers of low molecular weight (<10,000 daltons) and a mixture of polymer additives (300-1200 daltons). Mass spectrometry experiments were performed on a four sector mass spectrometer, a tandem quadrupole mass spectrometer and a time-of-flight instrument. High and low energy collision induced dissociation (CID) spectra were obtained by means of a four sector mass spectrometer and a tandem quadrupole instrument respectively. Surface induced dissociation (SID) spectra of peptides were obtained by means of a four sector mass spectrometer with a modified collision cell in the third field free region. Sequence data were generated by SID from protonated and cationated precursor ions of all four peptides analysed. Furthermore, broad metastable ion peaks were observed in the spectra, arising from fragmentation of precursor ions in the field free region between the electric sector and the magnetic sector of the second mass spectrometer. Field desorption was a good ionisation technique for the generation of molecular weight information from the polymer additives used. High energy eID was found to be more applicable than low energy eID to the structural determination of polymer additives as characteristic ions were observed in the spectra. Mechanisms have been proposed for the generation of some of the fragment ions observed. Ultraviolet-matrix assisted laser desorptionlionisation spectra of synthetic polymers of low molecular weight (<10,000 daltons) were used to calculate molecular weight averages. Furthermore, end group information was also obtained from CID spectra of the same polymer samples. End group and structural information was also obtained from polystyrene and a substituted polystyrene by means of FD-MS/MS.
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Goodwin, Lee. "Capillary electrophoresis-mass spectrometry and tandem mass spectrometry studies of ionic agrochemicals." Thesis, University of York, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.398906.

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Hsi, Kuang-Ying. "Peptide identification of tandem mass spectrometry from quadrupole time-of-flight mass spectrometers." Diss., [La Jolla] : University of California, San Diego, 2009. http://wwwlib.umi.com/cr/ucsd/fullcit?p1462246.

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Thesis (M.S.)--University of California, San Diego, 2009.
Title from first page of PDF file (viewed May 4, 2009). Available via ProQuest Digital Dissertations. Includes bibliographical references (p. 45-46).
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Frank, Ari Michael. "Algorithms for tandem mass spectrometry-based proteomics." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p3307704.

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Thesis (Ph. D.)--University of California, San Diego, 2008.
Title from first page of PDF file (viewed August 13, 2008). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 187-205).
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Beisken, Stephan Andreas. "Informatics for tandem mass spectrometry-based metabolomics." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708325.

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Liu, Xiumin. "Mass Spectrometry and Tandem Mass Spectrometry Analysis of Polymers and Polymer-Protein Interactions." University of Akron / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=akron1406838246.

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Chawner, Ross. "Combined tandem mass spectrometry and ion mobility spectrometry in proteome analyses." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/combined-tandem-mass-spectrometry-and-ion-mobility-spectrometry-in-proteome-analyses(3ba76f18-4703-4f6e-a97f-ee2b1dfb1deb).html.

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Proteomic studies aim to identify, quantify and characterise the full complement of proteins in a cell or organism under a defined set of conditions, and are important to our understanding of cellular mechanisms. However, such studies represent a major analytical challenge. A typical proteome analysis involves enzyme-mediated digestion of complex protein mixtures to yield an even more complex mixture of peptides. Combined reverse-phase liquid chromatography and tandem mass spectrometry is then traditionally utilised to ascertain sequence information from the characteristic peptide sequences. Analytical data derived for the peptides are employed as search terms in database searching of protein sequences derived from gene sequences. The extreme complexity of the peptide mixtures analysed means that additional novel approaches are required to fully interrogate the vast number of tandem mass spectra generated, assigning peptide identity and thereby helping to address demanding biological questions. The research reported here aims to further our understanding of both gas phase peptide/peptide fragment ion structure and peptide fragmentation behaviour using a combination of tandem mass spectrometry and ion mobility measurement.To facilitate the determination of peptide ion collision cross section, a novel standard, QCAL-IM, produced using the QconCAT strategy, has been developed to enable calibration of drift time in Travelling Wave Ion Mobility instruments. The standard facilitates empirical determination of the rotationally averaged collision cross section of any peptide/peptide fragment ion that lies within the calibration range encompassed. QCAL-IM was subsequently utilised to determine the collision cross section of a range of peptide ions produced by Lys-C and Lys-N proteolysis of ‘standard’ proteins. Data produced allowed the effect upon gas phase ion conformation through changing the location of the basic residue lysine within a peptide sequence to be assessed.The fragmentation behaviour of peptide ions produced by a variety of digestion regimes during both collision-induced dissociation (CID) and electron transfer dissociation (ETD) has also been extensively studied. The proteases trypsin and Lys-C are those typically utilised during proteomic studies and peptides produced by each have either the basic residues arginine or lysine at their carboxy-terminus. Secondary enzymatic treatment with the exoprotease carboxypeptidase B cleaves these basic residues from the C-terminus. Tandem mass spectrometric analysis of both tryptic/Lys-C peptides and their CBPB truncated analogue highlights that the dominant fragment ion series observed during both CID and ETD is determined, at least in part, by the location of such basic residues.Finally, studies were undertaken to investigate the factors which may promote/inhibit scrambling of peptide fragment ion sequence, which has recently been shown to take place during CID. The effect of modifying the gas phase basicity of the N-terminal amino acid residue is studied through a combination of derivatisation and synthesis of alternative peptide sequences. Increasing the gas phase basicity is shown to inhibit the observed sequence scrambling while promoting concomitant rearrangement/retention of a carboxyl oxygen at the C-terminus to give enhanced formation of bn+H2O product ion species.
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Roos, Felix Franz. "Algorithms for peptide identification by tandem mass spectrometry /." Zürich : ETH, 2006. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=16844.

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Books on the topic "Tandem mass spectrometry"

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Busch, Kenneth L. Mass spectrometry/ mass spectrometry: Techniques and applications of tandem mass spectrometry. Weinheim: VCH, 1988.

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Busch, Kenneth L. Mass spectrometry/mass spectrometry: Techniques and applications of Tandem mass spectrometry. New York, N.Y: VCH Publishers, 1988.

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Prasain, Jeevan K. Tandem mass spectrometry - applications and principles. Rijeka: InTech, 2012.

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Kinter, Michael, and Nicholas E. Sherman. Protein Sequencing and Identification Using Tandem Mass Spectrometry. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2000. http://dx.doi.org/10.1002/0471721980.

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E, Sherman Nicholas, ed. Protein sequencing and identification using tandem mass spectrometry. New York: John Wiley, 2000.

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Summerfield, Scott Graham. The tandem mass spectrometry of oligopeptides and proteins. [s.l.]: typescript, 1995.

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Davey, S. N. Development and applications of four sector tandem mass spectrometry. Manchester: UMIST, 1996.

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Dale, Vanessa Cordelia Meriel. The study of cyanobacterial toxins by means of tandem mass spectrometry. [s.l.]: typescript, 1994.

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Binns, Kathleen Leslie. Phosphopeptide mapping of axon guidance molecules by Nano-ESI tandem mass spectrometry. Ottawa: National Library of Canada, 2002.

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Butler, John M. Improved analysis of DNA short tandem repeats with time-of-flight mass spectrometry. Washington, D.C: U.S. Dept. of Justice, Office of Justice Programs, National Institute of Justice, 2001.

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Book chapters on the topic "Tandem mass spectrometry"

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Gross, Jürgen H. "Tandem Mass Spectrometry." In Mass Spectrometry, 415–78. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10711-5_9.

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Gross, Jürgen H. "Tandem Mass Spectrometry." In Mass Spectrometry, 539–612. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-54398-7_9.

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Westman-Brinkmalm, Ann, and Gunnar Brinkmalm. "Tandem Mass Spectrometry." In Mass Spectrometry, 89–103. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2008. http://dx.doi.org/10.1002/9780470395813.ch3.

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Ji, Quan, Zhimin Lin, Sixing Chen, and Zengli Yang. "Tandem Mass Spectrometry." In In Vitro Diagnostic Industry in China, 457–73. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-3110-1_27.

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Ciesla, Lukasz. "High-Resolution Mass Spectrometry (Tandem Mass Spectrometry)." In Determination of Target Xenobiotics and Unknown Compound Residues in Food, Environmental, and Biological Samples, 39–52. Boca Raton, FL : CRC Press, 2018. | Series: Chromatographic science series : a series of textbooks and reference books: CRC Press, 2018. http://dx.doi.org/10.1201/9780429446900-5.

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Gaskell, Simon J., and Kevin D. Ballard. "Hybrid Tandem Mass Spectrometry." In Mass Spectrometry in the Biological Sciences: A Tutorial, 29–58. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2618-2_2.

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Gage, Douglas A., Zhi-Heng Huang, and Charles C. Sweeley. "Characterization of Diacylglycerylphospholipids by Fast Atom Bombardment Tandem Mass Spectrometry." In Mass Spectrometry, 53–87. Boston, MA: Springer US, 1994. http://dx.doi.org/10.1007/978-1-4899-1748-5_2.

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Giorgi, Gianluca. "Mass Spectrometry and Tandem Mass Spectrometry: An Overview." In Detection of Chemical, Biological, Radiological and Nuclear Agents for the Prevention of Terrorism, 17–31. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9238-7_3.

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Smith, Richard D., Joseph A. Loo, and Charles G. Edmonds. "The Analysis of Biomolecules by Electrospray Ionization—Mass Spectrometry and Tandem Mass Spectrometry." In Mass Spectrometry, 37–98. Boston, MA: Springer US, 1992. http://dx.doi.org/10.1007/978-1-4899-1173-5_2.

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Staudenmann, Werner, and Peter James. "Interpreting Peptide Tandem Mass-Spectrometry Fragmentation Spectra." In Proteome Research: Mass Spectrometry, 143–66. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-56895-4_8.

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Conference papers on the topic "Tandem mass spectrometry"

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Chen, Ting. "Gene-finding via tandem mass spectrometry." In the fifth annual international conference. New York, New York, USA: ACM Press, 2001. http://dx.doi.org/10.1145/369133.369176.

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Chen, Xiaozhou, Yangli Du, Huamei Li, and Chuanle Xiao. "Protein identification algorithms for tandem mass spectrometry." In 5th International Conference on Advanced Computer Control. Southampton, UK: WIT Press, 2014. http://dx.doi.org/10.2495/icacc130391.

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Li, Xiang, Timothy Cornish, Scott Ecelberger, Stephanie A. Getty, and William B. Brinckerhoff. "Tandem mass spectrometry on a miniaturized laser desorption time-of-flight mass spectrometer." In 2016 IEEE Aerospace Conference. IEEE, 2016. http://dx.doi.org/10.1109/aero.2016.7500615.

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Dančík, Vlado, Theresa A. Addona, Karl R. Clauser, and James E. Vath. "De Novo peptide sequencing via tandem mass spectrometry." In the third annual international conference. New York, New York, USA: ACM Press, 1999. http://dx.doi.org/10.1145/299432.299471.

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El Jadid, Sara, Raja Touahni, and Ahmed Moussa. "Strategies of peptide identification using tandem Mass Spectrometry." In SMC '19: The Second Conference of the Moroccan Classification Society. New York, NY, USA: ACM, 2019. http://dx.doi.org/10.1145/3314074.3314078.

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He, Pinjie, and Kenli Li. "MIC-Tandem: Parallel X!Tandem Using MIC on Tandem Mass Spectrometry Based Proteomics Data." In 2015 15th IEEE/ACM International Symposium on Cluster, Cloud and Grid Computing (CCGrid). IEEE, 2015. http://dx.doi.org/10.1109/ccgrid.2015.31.

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Ollivier, S., D. Olivier, ACL Gerlach, L. Pellissier, M. Chollet-Krugler, F. Lohézic-Le Dévéhat, P. Clerc, J. Boustie, J.-L. Wolfender, and P.-M. Allard. "Comprehensive chemotaxonomy: mining data from tandem mass spectrometry of lichens." In 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. © Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-3399824.

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Akhtar, M. N., B. R. Southey, K. I. Porter, J. V. Sweedler, and S. L. Rodriguez-Zas. "Comparison of tandem mass spectrometry search methods to identify neuropeptides." In 2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops (BIBMW). IEEE, 2011. http://dx.doi.org/10.1109/bibmw.2011.6112530.

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Stott, William R., William R. Davidson, and Richard Sleeman. "High-specificity chemical detection of explosives by tandem mass spectrometry." In Applications in Optical Science and Engineering, edited by James M. Connelly and Shiu M. Cheung. SPIE, 1993. http://dx.doi.org/10.1117/12.142886.

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Bai, Wenruo, Jeffrey Bilmes, and William S. Noble. "Bipartite matching generalizations for peptide identification in tandem mass spectrometry." In BCB '16: ACM International Conference on Bioinformatics, Computational Biology, and Health Informatics. New York, NY, USA: ACM, 2016. http://dx.doi.org/10.1145/2975167.2975201.

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Reports on the topic "Tandem mass spectrometry"

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Voorhees, Kent J. Identification of Biomarkers in Bacteria by Pyrolysis - Tandem Mass Spectrometry. Fort Belvoir, VA: Defense Technical Information Center, September 1995. http://dx.doi.org/10.21236/ada300477.

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Loo, J. A., and R. D. Smith. Tandem mass spectrometry of multiply charged proteins with electrospray ionization. Office of Scientific and Technical Information (OSTI), June 1992. http://dx.doi.org/10.2172/10170406.

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McLuckey, S. A., D. E. Goeringer, and K. G. Asano. High explosives vapor detection by atmospheric sampling glow discharge ionization/tandem mass spectrometry. Office of Scientific and Technical Information (OSTI), February 1996. http://dx.doi.org/10.2172/225970.

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McLuckey, S. A., D. E. Goeringer, K. G. Asano, K. J. Hart, G. L. Glish, B. C. Grant, and D. M. Chambers. Atmospheric sampling glow discharge ionizataion and triple quadrupole tandem mass spectrometry for explosives vapor detection. Office of Scientific and Technical Information (OSTI), August 1993. http://dx.doi.org/10.2172/10184158.

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Kimble, Ashley, Derek Muensterman, Liliana Cahuas, Ivan Titaley, Jennifer Field, Anthony Bednar, and Lee Moores. Extraction and analysis of per- and polyfluoroalkyl Substances (PFAS) from Meals Ready-to-Eat (MRE) films using GC-MS and LC-MS/MS. Engineer Research and Development Center (U.S.), May 2023. http://dx.doi.org/10.21079/11681/47114.

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This work was in response to the Defense Logistic Agency’s (DLA) Subsistence Network Broad Agency Announcement, BAA-0003-16 addressing 2019 NDAA Section 329 that states packaging materials used for Meals Ready-to-Eat (MRE) that contact food products must be free of per- and polyfluoroalkyl substances (PFAS). This was addressed by determining the presence or absence of PFAS on MREs by extraction followed by gas chromatography mass spectrometry (GC-MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). Any samples positive for PFAS were quantitated using LC triple quadrupole (QqQ) MS at the US Army Engineering and Research Development Center (ERDC) and by high resolution quadrupole time-of-flight (qTOF) MS and GC-MS at Oregon State University (OSU).
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Shang, Zhirong, Pan Xie, Ke Pan, Jialin Liu, Wei Xu, Yue Hu, Li Tang, Qinghua Leng, Shuyu Liu, and Chengchuan He. Predictive value of fluorometric method and tandem mass spectrometry for hyperphenylalaninemia and its subtypes in China: A Systematic review and Meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, March 2024. http://dx.doi.org/10.37766/inplasy2024.3.0036.

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Beasley-Green, Ashley. Reference Measurement Procedure for the Absolute Quantification of Albumin in Urine Using Isotope Dilution-Liquid Chromatography-Tandem Mass Spectrometry (ID-LC-MS/MS). Gaithersburg, MD: National Institute of Standards and Technology, 2024. http://dx.doi.org/10.6028/nist.sp.1200-31.

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Moores, Lee C., P. U. Ashvin, I. Fernando, and Garret W. George. Synthesis of 2-Methoxypropyl Benzene for Epitope Imprinting. U.S. Army Engineer Research and Development Center, July 2022. http://dx.doi.org/10.21079/11681/44883.

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Harmful algal blooms (HABs) are occurring with increasing frequency and severity across the globe in part due to climate change and anthropogenic pollution (Bullerjahn et al. 2016). HABs produce several classes of toxins; however, microcystins (MCs) are the most commonly studied (Lone et al. 2015) and can be potent toxins with LD50s in the range of 50 μg/kg (Puddick et al. 2014). Sample analysis in laboratories, typically by high-pressure liquid chromatography tandem mass spectrometry (HPLC-MS/MS) or by Enzyme Linked Immunosorbent Assays (ELISAs) (USEPA 2015). These analytical techniques are highly sensitive and selective for the given toxins; however, the time it takes to collect, transfer, prepare, and analyze a sample before the data can be reported is significant; often, multiple days is the most expeditious.
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Young, Edward D. Development of Tandem, Double-Focusing, Electron Impact, Gas Source Mass Spectrometer for Measurement of Rare Double-Substituted Isotoplogues in Geochemistry. Office of Scientific and Technical Information (OSTI), July 2015. http://dx.doi.org/10.2172/1206480.

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Tucker, Mark L., Shimon Meir, Amnon Lers, Sonia Philosoph-Hadas, and Cai-Zhong Jiang. Elucidation of signaling pathways that regulate ethylene-induced leaf and flower abscission of agriculturally important plants. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597929.bard.

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The Problem: Abscission is a highly regulated process, occurring as a natural terminal stage of development, in which various organs are separated from the parent plant. In most plant species, the process is initiated by a decrease in active auxin in the abscission zone (AZ) and an increase in ethylene, and may be accelerated by postharvest or environmental stresses. Another potential key regulator in abscission is IDA (Inflorescence Deficient in Abscission), which was identified as an essential peptide signal for floral organ abscission in Arabidopsis. However, information is still lacking regarding the molecular mechanisms integrating all these regulators. In our previous BARD funded research we made substantial progress towards understanding these molecular events in tomato, and the study is still in progress. We established a powerful platform for analysis of genes for regulatory proteins expressed in AZ. We identified changes in gene expression for several transcription factors (TFs) directly linked to ethylene and auxin signaling and several additional regulatory proteins not so obviously linked to these hormones. Moreover, we demonstrated using a virus-induced gene silencing (VIGS) assay that several play a functional role in the onset of abscission. Based on these results we have selected 14 genes for further analysis in stably transformed tomato plants. All 14 genes were suppressed by RNA interference (RNAi) using a constitutive promoter, and 5 of them were also suppressed using an abscission-specific promoter. Transformations are currently at different stages of progress including some lines that already display an abscission phenotype. Objectives: We propose here to (1) complete the functional analysis of the stably transformed tomato plants with T2 lines and perform transcriptome analysis using custom abscission-specific microarrays; (2) conduct an indepth analysis of the role of IDA signaling in tomato leaf and flower abscission; (3) perform transcriptome and proteome analyses to extend the earlier gene expression studies to identify transcripts and proteins that are highly specific to the separation layer (i.e., target cells for cell separation) prior to the onset of abscission; (4) extend and compliment the work in tomato using a winnowed set of genes in soybean. Methodology: Next Generation Sequencing (NGS) of mRNA will be used to further increase the list of abscission-associated genes, and for preparation of a custom tomato abscission microarray to test altered gene expression in transgenic plants. Tandem mass spectrometry (LC-MS/MS) of protein extracts from leaf petiole, flower pedicel and their AZ tissues will be used to identify the proteome of the AZ before and during abscission. AZ-specific gene promoters will be used in stably transformed tomato plants to reduce non-target phenotypes. The bean pod mottle virus (BPMV) plasmid vectors will be used for VIGS analysis in soybean. Expected Contribution: Our study will provide new insights into the regulation of ethylene-induced abscission by further revealing the role of key regulators in the process. This will permit development of novel techniques for manipulating leaf and flower abscission, thereby improving the postharvest performance of agriculturally important crops.
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