Dissertations / Theses on the topic 'Systems biology investigation'
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Gormley, Padhhraig J. "An investigation of advanced data-driven identification methods for systems biology." Thesis, Queen's University Belfast, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.534735.
Full textGray, Kurtis N. "A Molecular Phylogeny of the Echeneoidea (Perciformes: Carangoidei) and an Investigation of Population Structuring Within the Echeneidae." W&M ScholarWorks, 2005. https://scholarworks.wm.edu/etd/1539617836.
Full textWang, Benjamin X. Ph D. Massachusetts Institute of Technology. "Investigation of two-component signaling systems in Pseudomonas aeruginosa and their roles in the mucus barrier." Thesis, Massachusetts Institute of Technology, 2021. https://hdl.handle.net/1721.1/130821.
Full textCataloged from the official PDF of thesis.
Includes bibliographical references.
All living things must be able to sense and respond to signals in their environment in order to grow and survive. For bacteria, a common means through which this occurs is via two-component signaling systems, which are typically comprised of a membrane-bound histidine kinase that senses external cues signals, and a cognate cytoplasmic response regulator that triggers changes in gene expression. The importance of these systems is highlighted by the fact that they have been identified in the vast majority of sequenced bacteria. However, despite their prevalence, much remains to be discovered about these sensory systems. In particular, both the actual processes that these systems control, along with the signals that they sense and respond to, remain poorly characterized in many cases.
In this work, I further characterize two-component signaling systems in the opportunistic pathogen Pseudomonas aeruginosa, which is most well-known for chronically infecting the lungs of patients with cystic fibrosis. I begin by screening a collection of in-frame deletion mutants of each histidine kinase in the PA14 strain against a dozen virulence-associated phenotypes, including different types of motility, biofilm formation, virulence factor production, and antibiotic resistance. Through this approach, I identify nearly two dozen of these proteins as important regulators of virulence. As P. aeruginosa is a human-associated mucosal pathogen, I next search for host-derived signals in mucus that act through these sensory systems in P. aeruginosa. I identify mucins and their associated glycans as signals that act through the RetS histidine kinase and the GacS-GacA two-component signaling system.
One major output of this signaling is the downregulation of the type VI secretion system, which suggests that mucin glycans may serve as host-derived "safety signals" that suppress microbial competition under non-dysbiotic conditions. Finally, I characterize the molecular mechanisms by which RetS inhibits GacS activity to better understand the unusual interactions between these two histidine kinases. Overall, this work underscores the diverse and important roles that two-component signaling systems play in bacteria, and begins to shed light on how microbes like P. aeruginosa utilize these systems to sense and respond to signals in the host.
by Benjamin X. Wang.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Biology
Dubaj, Vladimir, and n/a. "Novel optical fluorescence imaging probe for the investigation of biological function at the microscopic level." Swinburne University of Technology, 2005. http://adt.lib.swin.edu.au./public/adt-VSWT20060905.084615.
Full textPrice, Heather Leigh. "Investigation of larval sensory systems in the marine bryozoan, Bugula neritina." DigitalCommons@CalPoly, 2015. https://digitalcommons.calpoly.edu/theses/1410.
Full textHyder, Jennifer A. "An Investigation of the Effects of Increased Tidal Inundation, Competition, and Facilitation on Salt Marsh Systems." Thesis, University of South Florida, 2015. http://pqdtopen.proquest.com/#viewpdf?dispub=3700275.
Full textThe low-lying topographic nature of salt marshes makes plants in these communities particularly vulnerable to increased salinity and inundation exposure associated with sea level rise. Both increased salinity and inundation have been cited as major causes of reduced plant performance and survival in marsh and areas fringing marsh. In addition to limitations imposed by physical stress, interspecific interactions have also been shown to mediate the performance and survival of salt marsh and salt marsh fringing species. The Stress Gradient Hypothesis (SGH) postulates that species interactions shift from competitive to facilitative as stress levels increase and predicts that (a) the frequency and intensity of facilitative interactions increase as conditions become more stressful for plants and (b) the strength of competitive interactions increases as abiotic stress levels diminish. The SGH has been rigorously tested to examine how both the frequency and intensity of species interactions change under varying physical stress levels. Studies conducted in salt marsh systems have shown facilitation to be as strong of a driving force as competition in influencing plant performance and survival and have shown that while competition appears to be the pervasive force in the less physically stressful terrestrial zones fringing salt marshes, facilitation influences the performance and survival of species in harsher marsh areas. Under conditions of sea level rise, it remains unclear if the nature of interspecific interactions would shift as stress levels change. This research endeavors to examine the interplay between abiotic stresses and biotic interactions under conditions of increased salinity and inundation exposure.
The first study presented here investigated the effects of increased inundation and soil salinity associated with sea level rise on four salt marsh fringing species, and assesses how competition and facilitation impact survival of salt marsh fringing plant survival under these changing conditions. All plant species experienced reduced growth and photosynthetic inhibition below their current distributional positions, both in the presence and absence of neighboring above ground vegetation. The findings also signal a potential shift in the nature of interspecific interactions from competition to facilitation to neutral as plants begin to experience increased salt and inundation exposure.
The second study aimed to disentangle the effects of increased soil salinity and increased soil moisture on four salt marsh fringing species, and to examine the effects of plant neighbors. The results showed that fringe plants exposed to increased inundation experienced a two-fold reduction in performance and survival over 750 g pure salt addition, suggesting that inundation may be a more important limiting factor than salinity with rising sea levels. Landward transplants at the forest-fringe margin exposed to lower soil salinity and decreased inundation exhibited a three-fold increase in performance and survival when compared to controls. Neighbor manipulation studies, which consisted of trimming neighboring vegetation to ground level, again suggested that interspecific interactions in salt marsh fringing species may shift from competitive to facilitative with climate-induced sea level rise. Overall, our findings suggest that salt marsh fringing species may not be able to tolerate changing conditions associated with sea level rise and their survival may hinge on their ability to migrate towards higher elevations.
The final experiment tested the Stress Gradient Hypothesis and investigated the relative importance of facilitation and competition in a salt marsh system under varying stress levels. This study also ascertained whether salt or inundation exposure is the primary influence on salt marsh plant performance and survival. As in previous studies, our findings suggest that many salt marsh plants don't require, but merely tolerate harsher abiotic conditions. The results showed that plants at higher elevations were depressed by strong competitive pressure from neighboring fringe species while plants at lower elevations benefited from the presence of neighbors. Collectively, the results of these studies indicate that species interactions are an integral driver of plant distribution in salt marsh communities. Furthermore, our findings indicate that changing stress levels may not always result in a shift in the nature of interspecific interactions. These studies have endeavored to show that the interplay between competition and facilitation interacts with physical processes to determine the growth and performance of both fringe and marsh plant species. The paucity of studies examining the roles of species interactions and changing abiotic stress levels on multiple salt marsh and salt marsh fringing species warrants the need for additional research. The responses of salt marsh and salt marsh fringing species to sea level rise can not only serve as very valuable and sensitive indictors of climate change, but will also aid in predicting the future location of the marsh-fringe-forest ecotone, which is predicted to shift inland as sea levels continue to rise.
Britton, Oliver Jonathan. "Combined experimental and computational investigation into inter-subject variability in cardiac electrophysiology." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:6299240d-0528-4662-8e1f-5025f39e730f.
Full textParikh, Jaimit B. "Theoretical Investigation of Intra- and Inter-cellular Spatiotemporal Calcium Patterns in Microcirculation." FIU Digital Commons, 2015. http://digitalcommons.fiu.edu/etd/1927.
Full textChee, Meng May. "Investigations into telomere biology in systemic sclerosis." Thesis, University of Glasgow, 2012. http://theses.gla.ac.uk/3615/.
Full textWilcock, Paul. "A systems biology approach for investigating oral squamous cell carcinoma (OSCC)." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/a-systems-biology-approach-for-investigating-oral-squamous-cell-carcinoma-oscc(8ec3728b-1928-450f-b467-76996fa970fb).html.
Full textYen, Jennifer Lee. "Investigating the zebrafish system for modelling cancer genomics and biology." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648122.
Full textXie, Zhi. "Modelling genetic regulatory networks: a new model for circadian rhythms in Drosophila and investigation of genetic noise in a viral infection process." Phd thesis, Lincoln University. Agriculture and Life Sciences Division, 2007. http://theses.lincoln.ac.nz/public/adt-NZLIU20070712.144258/.
Full textMiu, Peter. "An electrophysiological investigation of monosialoganglioside in the mammalian central nervous system." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41186.
Full textThe most probable mechanism underlying the selective enhancement of excitatory inputs by GM1 is an increase in glutamate release, since the amplitudes and frequency of spontaneous miniature postsynaptic responses, recorded either in the presence or absence of presynaptic cell firing, were cosistently increased. When L-glutamate was applied iontophoretically in the dendritic region, GM1 transiently potentiated the postsynaptic glutamate currents, thus further indicating a GM1-induced enhancement of glutamatergic transmission. In contrast, both spontaneous and evoked inhibitory postsynaptic responses were suppressed by GM1. This effect is dependent on changes in excitatory inputs to inhibitory interneurons because in the presence of tetrodotoxin and/or kynurenic acid, GM1 did not alter the amplitude of the monosynaptic IPSPs or the frequency of spontaneous miniature IPSCs.
Both the GM1-induced inward current and the reduction of postsynaptic HVA Ca$ sp{2+}$ currents were antagonised by kynurenic acid, suggesting that these effects might be caused by glutamate receptor activation. By raising intraneuronal Ca$ sp{2+}$ concentration, the potentiated glutamate release would trigger Ca$ sp{2+}$-dependent Ca$ sp{2+}$ inactivation, and thus explain the reduction in HVA Ca$ sp{2+}$ currents.
In conclusion, most of the GM1 actions observed in this project can be explained on the basis of a GM1-induced facilitation of excitatory transmission, mediated especially via enhanced glutamate release.
Cizmeci, Deniz. "A systems biology approach to investigating host-pathogen interactions in infection with Burkholderia pseudomallei." Thesis, University of Exeter, 2017. http://hdl.handle.net/10871/29963.
Full textFinneran, James Joseph. "An experimental and theoretical investigation of the mechanics of the goldfish peripheral auditory system /." Connect to resource, 1997. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1230661897.
Full textSherwood, Amanda R. "INVESTIGATING THERAPEUTIC OPTIONS FOR LAFORA DISEASE USING STRUCTURAL BIOLOGY AND TRANSLATIONAL METHODS." UKnowledge, 2013. http://uknowledge.uky.edu/biochem_etds/13.
Full textShi, Huilin. "Investigation of Common Bases of Sympathetic Nervous System and Neuroblastoma Development." University of Toledo Health Science Campus / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=mco1244058100.
Full textFrawley, Laura E. (Laura Elizabeth). "Investigation of the role of polyploidization in glial cells during the development of the drosophila nervous system." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/117882.
Full textCataloged from PDF version of thesis.
Includes bibliographical references.
Organogenesis is a complex process encompassing cell determination, cell differentiation, cell proliferation, and cell size regulation. The proper orchestration of these events ensures that each organ is scaled correctly and can function properly. Polyploidization is a process by which cells increase their DNA content and is used across species to generate large cells. Our lab had previously determined that subperineurial glia (SPG) of the Drosophila melanogaster nervous system become polyploid by both the endocycle and endomitosis. These are two cell cycle variants employed to produce polyploid cells that differ in the latter undergoing some aspects of mitosis but not cytokinesis. Polyploidization of the SPG is critical for blood-brain barrier (BBB) function. Here, we determined that the developmental switch from endocycling to endomitotic SPG occurs in about 70% of SPG in the brain lobes by the second larval instar. The SPG in the ventral nerve cord and peripheral nervous system solely endocycle. We demonstrated that both the Notch signaling pathway and the String Cdc25 phosphatase are critical in determining whether SPG endocycle or endomitose. Experiments manipulating the percentage of cells that are endocycling versus endomitotic highlight key differences between endocycling and endomitotic SPG. We find that endomitotic SPG cells are capable of achieving higher ploidy and cell area values than endocycling cells and are essential to the integrity of the BBB. Strikingly, we find that endocycling SPG within the ventral nerve cord retain the ability to undergo endomitosis when the Notch signaling pathway or the String Cdc25 phosphatase are altered. Further, we showed that a second glial cell type in the peripheral nervous system, wrapping glia (WG), is polyploid and determined that total WG ploidy correlates with nerve length. Interestingly, when WG ploidy was reduced, we found that axonal ensheathment is defective. We also established that the three WG per peripheral nerve differentially contribute to overall ploidy. Axonal ensheathment throughout the entire nerve seems to be dependent on position along the anterior-posterior larval body axis. Finally, we find that reduction of DNA replication components causes reduced WG ploidy only in longer peripheral nerves.
by Laura E. Frawley.
Ph. D.
Paton, R. C. "Some investigations into the use and meaning of system in biological education." Thesis, University of Leeds, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233889.
Full textBeaulieu, Angela Denise. "Investigations of milk protein and lipid synthesis utilizing an in vitro bovine mammary cell culture system /." The Ohio State University, 1994. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487858417984017.
Full textRobinson, David. "An investigation into novel molecules involved in the development of the nervous system of Drosophila melanogaster." Thesis, University of Greenwich, 2015. http://gala.gre.ac.uk/18054/.
Full textDavis, Jon Franklin. "A Functional, Anatomical, and Molecular Investigation of Natural Reward: Sexual Plasticity and Limbic System." Cincinnati, Ohio : University of Cincinnati, 2005. http://www.ohiolink.edu/etd/view.cgi?acc%5Fnum=ucin1123831570.
Full textTitle from electronic thesis title page (viewed Mar. 23, 2006). Includes abstract. Keywords: limbic, reward, plasticity. Includes bibliographical references.
Adamska-Venkatesh, Agnieszka [Verfasser]. "Spectroscopic investigations of [FeFe] hydrogenases and related model systems / Agnieszka Adamska-Venkatesh." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2015. http://d-nb.info/1072224569/34.
Full textRossmanith, Eva. "Breeding biology, mating system and population dynamics of the Lesser Spotted Woodepcker (Picoides minor) : combining empirical and model investigations." Phd thesis, Universität Potsdam, 2005. http://opus.kobv.de/ubp/volltexte/2005/532/.
Full textIn the field studies I collected basic demographic data of reproductive success and mortality. Moreover, breeding biology and behaviour were investigated in detail. My results showed a significant decrease of the reproductive success with later timing of breeding, caused by deterioration in food supply. Moreover, mate fidelity was of benefit, since pairs composed of individuals that bred together the previous year started earlier with egg laying and obtained a higher reproductive success. Both sexes were involved in parental care, but the care was only shared equally during incubation and the early nestling stage. In the late nestling stage, parental care strategies differed between sexes: Females considerably decreased feeding rate with number of nestlings and even completely deserted small broods. Males fed their nestlings irrespective of brood size and compensated for the females absence. The organisation of parental care in the Lesser Spotted Woodpecker is discussed to provide the possibility for females to mate with two males with separate nests and indeed, polyandry was confirmed.
To investigate the influence of the observed flexibility in the social mating system on the population persistence, a stochastic individual-based model simulating the population dynamics of the Lesser Spotted Woodpecker was developed, based on empirical results. However, pre-breeding survival rates could not be obtained empirically and I present in this thesis a pattern-oriented modelling approach to estimate pre-breeding survival rates by comparing simulation results with empirical pattern of population structure and reproductive success on population level. Here, I estimated the pre-breeding survival for two Lesser Spotted Woodpecker populations on different latitudes to test the reliability of the results.
Finally, I used the same simulation model to investigate the effect of flexibility in the mating system on the persistence of the population. With increasing rate of polyandry in the population, the persistence increased and even low rates of polyandry had a strong influence. Even when presuming only a low polyandry rate and costs of polyandry in terms of higher mortality and lower reproductive success for the secondary male, the positive effect of polyandry on the persistence of the population was still strong.
This thesis greatly helped to increase the knowledge of the autecology of an endangered woodpecker species. Beyond the relevance for the species, I could demonstrate here that in general flexibility in mating systems are buffer mechanisms and reduce the impact of environmental and demographic noise.
Der Schutz von Arten ist eine der Hauptaufgaben des Naturschutzes. Für die Erstellung von Schutzkonzepten sind Informationen zur Autökologie der Zielart notwendige Voraussetzung. Der Kleinspecht (Picoides minor) ist in vielen Teilen seines Verbreitungsgebietes bestandsbedroht, das Wissen zur Biologie und Verhalten der Art ist jedoch lückenhaft. Ziel meiner Arbeit war es daher, demographische Parameter der Populationsdynamik des Kleinspechts zu erfassen, die als Grundlage für Populationsgefährdungsanalysen benötigt werden. Da Untersuchungen in Schweden eine gewisse Flexibilität im Paarungssystem des Kleinspechts zeigten, sollte darüber hinaus das Paarungssystem und sein Einfluss auf die Persistenz der Population untersucht werden.
Die Arbeit umfasste eine Reihe von methodischen Ansätzen, von empirischen Untersuchungen an einer Kleinspechtpopulation im hessischen Vordertaunus über die Aufbereitung von empirischen Daten bis hin zur Entwicklung und Auswertung eines stochastischen individuenbasierten Modells zur Simulation der Populationsdynamik.
Die Ergebnisse der empirischen Untersuchung zeigten eine Abnahme des Reproduktionserfolgs mit fortschreitendem Legebeginn. Die Zusammensetzung der Nestlingsnahrung ließ vermuten, dass dies durch eine Verschlechterung der Nahrungsversorgung begründet war. Paartreue war bei der Reproduktion von Vorteil, da Individuen, die schon im vorherigen Jahr zusammen gebrütet hatten, einen früheren Legebeginn und damit einen höheren Fortpflanzungserfolg aufwiesen als neu formierte Paare. Beide Geschlechter investierten in die Brutpflege, jedoch war die Aufteilung nur während der Bebrütung der Eier und in der ersten Hälfte der Nestlingsperiode gleichmäßig. In der späten Nestlingsperiode konnten geschlechtsspezifische Strategien im elterlichen Investment identifiziert werden: die Weibchen verringerten die Versorgungsrate in Abhängigkeit des Wertes der Brut - gemessen in der Zahl der Nestlinge - und gaben die Versorgung kleiner Bruten ganz auf. Die Männchen dagegen kompensierten dieses Verhalten, so dass auch von den Weibchen verlassene Bruten erfolgreich waren. Interessanterweise konnte mehrmals die Verpaarung von einem Weibchen mit zwei Männchen beobachtet werden. Das Auftreten dieses polyandrischen Paarungssystems wird in der Arbeit als Resultat der Aufteilung der Brutpflege diskutiert.
Die bestätigte Flexibilität im Paarungssystem könnte Einfluss auf die Persistenz der Population haben. Die Persistenz von Populationen kann jedoch nicht empirisch gemessen werden. Daher entwickelte ich ein individuen-basiertes stochastisches Modell zur Simulation der Populationsdynamik des Kleinspechts, dass auf den empirischen Daten basiert. Allerdings fehlten Überlebensraten der ausgeflogenen Jungvögel, die im Feld nicht ermittelt werden kann. Daher testete ich hier eine Methode, die durch den Vergleich von Simulationsergebnissen mit eigenen empirischen Daten zur Populationsstruktur und zum Reproduktionserfolg auf der Ebene der Gesamtpopulation die Überlebensrate der Jungvögel abschätzt. Die Überlebensraten wurde zusätzlich für eine Population des Kleinspechtes ermittelt, deren Datengrundlage aus Freilandstudien in Schweden stammten. Durch den Vergleich der Raten für die beiden Populationen konnte die Aussagefähigkeit des Modells und die Güte der Abschätzungen untersucht werden. Im letzten Teil meiner Arbeit nutzte ich das Modell schließlich, um die Auswirkungen des Paarungssystems auf die Überlebensfähigkeit der Population zu untersuchen. Im Modell konnte ein Weibchen polyandrisch sein, wenn es gute Brutbedingungen hatte und das Geschlechterverhältnis zum Männchen hin verschoben war. Zusätzlich variierte ich die Wahrscheinlichkeit, dass unter diesen Umständen Polyandrie auftritt. Im Model wurden 3 Szenarien getestet: (i) strenge Monogamie, (ii) gelegentliche Polyandrie und (iii) gelegentliche Polyandrie unter der Annahme von Kosten für das sekundäre Männchen in Form von höherer Mortalität und geringerem Reproduktionserfolg. Es zeigte sich, dass selbst sehr geringe Polyandrieraten und die Annahme von Kosten noch einen deutlichen positiven Einfluss auf die Persistenz der Population ausüben. Die Flexibilität im Paarungssystem dient damit als Puffermechanismus gegen demographisches Rauschen und Umweltrauschen.
Diese Arbeit trät dazu bei, die Autökologie des Kleinspechts besser zu verstehen und ist damit wichtige Grundlage für Schutzkonzepte in Mitteleuropa. Über die artspezifische Bedeutung hinaus, leistet die Arbeit einen Beitrag zur Untersuchung von Methoden zur Abschätzung fehlender demographischer Parameter sowie zur Identifizierung von Puffermechanismen. Eine wichtige Schlussfolgerung meiner Arbeit ist es, dass die Flexibilität artspezifischen Verhaltens in zukünftigen Populationsgefährdungsanalysen integriert werden sollte, um die Qualität von Prognosen zur Persistenz von Populationen zu verbessern.
Nyrén, Karl. "Phylogenetic analysis of secretion systems in Francisellaceae and Legionellales : Investigating events of intracellularization." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-448062.
Full textKingston, Demelza. "Investigating central nervous system trypanosomosis in working equids in The Gambia." Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/30682/.
Full textGoschorska, Maja. "Investigating the mechanisms of cell competition in mammals using in vitro systems." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/290214.
Full textHille, Frank [Verfasser]. "Investigation of Spacer Acquisition Mechanisms in Type V-A CRISPR-Cas Systems / Frank Hille." Berlin : Humboldt-Universität zu Berlin, 2020. http://d-nb.info/1215570341/34.
Full textBates, Nicholas Robert. "Investigation of the physical and biological controls of the oceanic CO2 system in the Sargasso Sea." Thesis, University of Southampton, 1995. https://eprints.soton.ac.uk/358400/.
Full textCharles, Sarah Lucy. "Investigations into the physiological actions of nitric oxide in the nervous systems of the ring dove and the rat." Thesis, University of Central Lancashire, 1996. http://clok.uclan.ac.uk/18873/.
Full textMuhammad, Nefertiti. "INVESTIGATING THE PORE COMPOSITION OF THE CHLOROPLAST TWIN ARGININE TRANSPORT SYSTEM." Miami University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami1543609222190572.
Full textKis, Zoltan. "Development of novel synthetic and systems biology tools for investigating and obviating the effect of atherogenic blood flow on vascular cells." Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/33249.
Full textSatler, Jordan. "Do ecological communities co-diversify? An investigation into the Sarracenia alata pitcher plant system." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1467855198.
Full textDunn, Karen. "Investigation into the Nrf2 signalling system dynamics and its crosstalk with the NF-κB signalling pathway at the single cell level." Thesis, University of Liverpool, 2013. http://livrepository.liverpool.ac.uk/16373/.
Full textLalovic, Aleksandra. "Genetic studies of suicidal behaviour : investigation of genes involved in the serotonergic system and cholesterol metabolism." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79022.
Full textGärdes, Astrid [Verfasser]. "A bilateral model system for the molecular investigation of diatom-bacteria interactions / Astrid Gärdes." Bremen : IRC-Library, Information Resource Center der Jacobs University Bremen, 2010. http://d-nb.info/1035032279/34.
Full textBoopathy, Sivakumar. "Investigating Structural and Functional Defects in ALS-causing Profilin 1 Variants." eScholarship@UMMS, 2009. http://escholarship.umassmed.edu/gsbs_diss/923.
Full textBoopathy, Sivakumar. "Investigating Structural and Functional Defects in ALS-causing Profilin 1 Variants." eScholarship@UMMS, 2017. https://escholarship.umassmed.edu/gsbs_diss/923.
Full textMüller, Britta Katrin [Verfasser]. "Systems biological investigation of aerobic and anaerobic aromatic catabolism in the bacterium Aromatoleum aromaticum EbN1 / Britta Katrin Müller." Braunschweig : Technische Universität Braunschweig, 2016. http://d-nb.info/1175818380/34.
Full textBakhti, Mostafa. "Investigation of myelin membrane adhesion and compaction in the central nervous system." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2012. http://hdl.handle.net/11858/00-1735-0000-000D-FB5C-B.
Full textMussard, Chase W. "In Vitro Investigation of the Effect of Exogenous Ubiquitin on Processes Associated with Atherosclerosis." Digital Commons @ East Tennessee State University, 2016. https://dc.etsu.edu/honors/327.
Full textRobinson, Jeffrey G. "An investigation of the seasonal and spatial occurrence of coliform bacteria in a distribution system." Virtual Press, 1992. http://liblink.bsu.edu/uhtbin/catkey/845953.
Full textDepartment of Biology
Steyn, Natassja Lise. "Investigating the localisation of the ESX-3 secretion system in Mycobacterium smegmatis." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71959.
Full textENGLISH ABSTRACT: Mycobacterium tuberculosis is a pathogenic organism that infects a third of the world’s population and causes approximately 2 million deaths per year. Extensive research has been done on this pathogen, however our knowledge of the mechanisms of pathogenicity remain limited. The M. tuberculosis genome contains five ESAT-6 gene cluster regions, ESX-1 to 5, which encode specialized type VII secretion systems. These secretion systems are known to secrete members of the ESAT-6/CFP-10 and PE/PPE protein families, some of which contribute to the pathogenicity and phagosomal escape of the pathogen. ESX-3 has been shown to be essential for in vitro growth and survival of M. tuberculosis. The expression of ESX-3 in M. tuberculosis is regulated by IdeR and Zur, in response to intracellular iron and zinc concentrations, respectively. Interestingly, ESX-3 is not essential for the growth and survival of the saprophytic organism M. smegmatis. In this study, we aimed to identify the subcellular localisation of the individual components of the ESX-3 secretion system in the non-pathogenic, fast-growing organism M. smegmatis. The esx conserved component (ecc) genes from ESX-3 were expressed from the episomal expression vector pDMNI as fusion proteins with green fluorescent protein (GFP). MSMEG_0615 (eccA3), MSMEG_0616 (eccB3), MSMEG_0623 (eccD3) and MSMEG_0626 (eccE3) were successfully cloned into pDMNI and expression of fusion proteins was confirmed by Western blotting for MSMEG_0615-GFP, MSMEG_0616-GFP and MSMEG_0626-GFP in M. smegmatis. In the M. smegmatis ESX-3 knock-out (with MSMEG_0615 to MSMEG_0626 deleted) expression was confirmed for MSMEG_0615-GFP and MSMEG0626-GFP. Fluorescent microscopy determined that MSMEG_0615-GFP localised to a single mycobacterial pole in both strains. MSMEG_0616-GFP and MSMEG_0626-GFP were found to be membrane associated in M. smegmatis, while MSMEG_0626-GFP was found to be membrane associated in the M. smegmatis ESX-3 knock-out. The unipolar localisation of MSMEG_0615-GFP suggests that the assembled ESX-3 secretion system apparatus is situated at a single pole in M. smegmatis. Therefore, we hypothesize that MSMEG_0615 might act as a recruiter protein that is involved in the assembly of ESX-3 at the mycobacterial pole.
AFRIKAANSE OPSOMMING: Mycobacterium tuberculosis is ‘n patogene organisme wat ‘n derde van die wêreld se bevolking infekteer en eis jaarliks 2 miljoen lewens deur tuberkulose. Ten spyte van uitgebreide navorsing, is daar min kennis oor die meganismes van patogenisiteit van hierdie organisme. Die M. tuberculosis genoom bevat vyf duplikasies van die ESAT-6 geen groep gebiede, ESX-1 tot 5, wat kodeer vir gespesialiseerde Tipe VII sekresie sisteme. Hierdie sekresie sisteme is bekend vir die sekresie van lede van die ESAT-6/CFP-10 en PE/PPE proteïen families, waarvan sommige bydra tot die patogenisieit en fagosomale ontsnapping van hierdie organisme. ESX-3 is noodsaaklik vir die in vitro groei en oorlewing van M. tuberculosis. Die uitdrukking van ESX-3 in M. tuberculosis word gereguleer deur IdeR en Zur in reaksie op intrasellulêre yster en sink konsentrasies, onderskeidelik. ESX-3 word nie benodig vir die groei en oorlewing van die saprofitiese organisme M. smegmatis nie. Hierdie studie was gemik om die sub-sellulêre lokalisering van ESX-3 te identifiseer in die niepatogeniese en vinnig-groeiende organisme, M. smegmatis. Die “esx conserved component” (ecc) gene van ESX-3 is uitgedruk vanaf die episomale uitdrukkingsvektor pDMNI as gekombineerde proteïene met die groen fluoreserende proteïen (GFP). MSMEG_0615 (eccA3), MSMEG_0616 (eccB3), MSMEG_0623 (eccD3) en MSMEG_0626 (eccE3) is suksesvol gekloneer en die uitdrukking van die gekombineerde proteïene is bevestig deur Western oordrag vir MSMEG_0615-GFP, MSMEG_0616-GFP en MSMEG_0626-GFP in M. smegmatis. In die M. smegmatis ESX-3 uitklopmutant (met MSMEG_0615 tot MSMEG_0626 uitgeslaan) is uitdrukking bevestig vir MSMEG_0615-GFP en MSMEG0626-GFP. Fluoresensie mikroskopie het bepaal dat MSMEG_0615-GFP gelokaliseer is by ‘n enkele mikobakteriese pool in beide stamme. MSMEG_0616-GFP en MSMEG_0626-GFP was membraan-geassosieerd in M. smegmatis, terwyl en MSMEG_0626-GFP geassosieer het met die membraan in die M. smegmatis uitklopmutant. MSMEG_0615 het gelokaliseer by ‘n enkele pool in M. smegmatis en dit dui aan dat die saamgestelde ESX-3 sekresie sisteem apparaat slegs by ‘n enkele pool voorkom in M. smegmatis. Ons hipotiseer dat MSMEG_0615 dalk mag optree as ‘n werwer proteïen wat betrokke is by die samestelling van die ESX-3 sekresie sisteem by die mikrobakteriese pool.
Stellenbosch University
Ternes, Svenja [Verfasser]. "Investigation of the endocannabinod system using in vivo and in vitro models / Svenja Ternes." Bonn : Universitäts- und Landesbibliothek Bonn, 2013. http://d-nb.info/1054691975/34.
Full textWhelan, Jillian Nicole. "Investigation of Respiratory Syncytial Virus Structural Determinants and Exploitation of the Host Ubiquitin System." Scholar Commons, 2016. http://scholarcommons.usf.edu/etd/6431.
Full textPark, Minwoo. "2',3'-Cyclic nucleotide 3'-phosphodiesterase : investigation of interaction with Fyn tyrosine kinase during the development of nervous system, and mitochondrial import of CNP2 isoform." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81423.
Full textFirst, a possible interaction of CNP with Fyn tyrosine kinase during myelination is investigated. Fyn is an important factor known to be active during the process of myelination, and CNP contains a number of possible tyrosine phosphorylation sites. Furthermore, their presence in an isolated domain of cell membrane called lipid rafts, further strengthened the possibility of interaction. However, no evidence was found which could support the possibility, neither in new born mouse brain nor in in-vitro experiment using transfected cells expressing Fyn and CNP.
Secondly, the role of CNP2 isoform in mitochondria is investigated. CNP2 is found to be associated with mitochondria, and its 20 amino acid segment at the N-terminus possesses characteristics of a mitochondrial import signal. Furthermore, two known sites of serine phosphorylation in the N terminus of CNP2 show influence on mitochondrial localization of the protein. However, results collectively suggests that CNP2 is not imported into mitochondria, as pulse chase did not show the typical processing of what was suspected to be the N-terminal import signal sequence. Furthermore, CNP was degraded when partially purified mitochondria was subjected to protease action, showing that CNP is not enclosed by mitochondrial membranes. Two serine residues at positions 9 and 22 in the N-terminus of CNP2 are likely phosphorylated by both PKA and PKC, and is responsible for the decrease in mitochondrial localization of CNP2.
Kwok, Chiu Wai [Verfasser], and Uwe [Akademischer Betreuer] Strähle. "In vitro cell culture systems for the investigation of the morphogen Sonic hedgehog (Shh) / Chiu Wai Kwok ; Betreuer: Uwe Strähle." Heidelberg : Universitätsbibliothek Heidelberg, 2011. http://d-nb.info/1179782674/34.
Full textChoi, Sungwook. "Investigation of LIN-28 Function in Somatic Gonadal Development and Fertility, and Characterization of the LIN-28 Isoforms in C. elegans Hermaphrodites." eScholarship@UMMS, 2018. https://escholarship.umassmed.edu/gsbs_diss/991.
Full textStephan, Till [Verfasser]. "Investigation of the mitochondrial contact site and cristae organizing system and its role in cristae formation / Till Stephan." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1220504513/34.
Full textNagel, Maximilian [Verfasser], Marc [Akademischer Betreuer] Spehr, and Ivan [Akademischer Betreuer] Manzini. "Physiological investigation of sensory signaling mechanisms in the mouse accessory olfactory system / Maximilian Nagel ; Marc Spehr, Ivan Manzini." Aachen : Universitätsbibliothek der RWTH Aachen, 2019. http://d-nb.info/1211487830/34.
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