Dissertations / Theses on the topic 'Sydney history'
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Lees, Jennifer Anne, University of Western Sydney, of Arts Education and Social Sciences College, and School of Humanities. "Eisteddfoditis : the significance of the City of Sydney Eisteddfod in Australian cultural history 1933-1941." THESIS_CAESS_HUM_Lees_J.xml, 2003. http://handle.uws.edu.au:8081/1959.7/714.
Full textDoctor of Philosophy (PhD)
Lees, Jennifer Anne. "Eisteddfoditis : the significance of the City of Sydney Eisteddfod in Australian cultural history 1933-1941 /." View thesis, 2003. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20051109.114852/index.html.
Full textA thesis submitted in requirement for the degree of Doctor of Philosophy - Communication & Media, University of Western Sydney, 2003. Bibliography : leaves 350-372.
Steele, Jeremy Macdonald. "The aboriginal language of Sydney a partial reconstruction of the indigenous language of Sydney based on the notebooks of William Dawes of 1790-91, informed by other records of the Sydney and surrounding languages to c.1905 /." Master's thesis, Electronic version, 2005. http://hdl.handle.net/1959.14/738.
Full textBibliography: p. 327-333.
Introduction -- Sources and literature -- The notebooks -- Manuscripts and databases -- Neighbouring languages -- Phonology -- Pronouns -- Verbs -- Nouns -- Other word classes -- Retrospect and prospect.
'Wara wara!" - 'go away' - the first indigenous words heard by Europeans at the time of the social upheaval that began in 1788, were part of the language spoken by the inhabitants around the shores of Port Jackson from time immemorial. Traces of this language, funtionally lost in two generations, remain in words such as 'dingo' and 'woomera' that entered the English language, and in placenames such as 'Cammeray' and 'Parramatta'. Various First Fleeters, and others, compiled limited wordlists in the vicinity of the harbour and further afield, and in the early 1900s the surveyor R.H. Mathews documented the remnants of the Dharug language. Only as recently as 1972 were the language notebooks of William Dawes, who was noted by Watkin Tench as having advanced his studies 'beyond the reach of competition', uncovered in a London university library. The jottings made by Dawes, who was learning as he went along, are incomplete and parts defy analysis. Nevertheless much of his work has been confirmed, clarified and corrected by reference to records of the surrounding languages, which have similar grammatical forms and substantial cognate vocabulary, and his verbatim sentences and model verbs have permitted a limited attempt at reconstructing the grammar.
Mode of access: World Wide Web.
xxi, 333 p. ill. (some col.), maps (some col.), ports
Butler-Bowdon, Caroline School of Planning and Urban Development Faculty of Built Environment UNSW. "Sydney apartments: the urban, cultural and design identity of the alternative dwelling 1900-2008." Awarded By:University of New South Wales. School of Planning and Urban Development, 2009. http://handle.unsw.edu.au/1959.4/44495.
Full textThornley, Clare A. "The Royal Philharmonic Society of Sydney: the rise and fall of a musical organisation." University of Sydney, 2004. http://hdl.handle.net/2123/1414.
Full textThe Royal Philharmonic Society of Sydney, formed as the Sydney Philharmonic Society in 1885, represented the rich tradition of amateur choral organisations present in Sydney in the nineteenth and early twentieth centuries. Under the strong leadership of two of their conductors, Roberto Hazon and Joseph Bradley, the Philharmonic Society presented the Sydney and Australian premieres of many choral works, engaged the services of many international vocal soloists, performed for full houses, and was invited to perform at many important civic and state events. Yet this organisation has been forgotten by history and the Sydney music community. Although many issues contributed to the decline of this amateur organisation, the strongest factors included the Philharmonic’s inability to maintain consistency in their leadership in later years, a change in general musical trends from amateur vocal performances to professional orchestral concerts, an increase in competition from other entertainments, the establishment of the ABC, and an ongoing lack of support from the city and state governments. These were further exacerbated by the lack of support from members of the Sydney press, particularly the Sydney Morning Herald. Therefore, an in-depth study into the story of the Royal Philharmonic Society of Sydney not only uncovers the history of a forgotten music organisation, it also contributes to a deeper understanding of the musical performance culture of the late nineteenth and early twentieth centuries in Sydney.
Finnane, Gabrielle. "Second nature : artifice and history in film /." View thesis View thesis, 1996. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030909.115616/index.html.
Full textHope, Cathy. "A history of the Sydney and Melbourne Film Festivals, 1945-1972 negotiating between culture and industry /." Connect to this title online Connect to this title online (alternate address), 2004. http://cicada.canberra.edu.au/public/adt-AUC20050630.130907/.
Full textHope, Cathy, and n/a. "A History of the Sydney and Melbourne Film Festivals, 1945-1972: negotiating between culture and industry." University of Canberra. Creative Communication, 2004. http://erl.canberra.edu.au./public/adt-AUC20050630.130907.
Full textWilkenfeld, George. "The electrification of the Sydney energy system, 1881-1986." Phd thesis, Australia : Macquarie University, 1989. http://hdl.handle.net/1959.14/33547.
Full textBibliography: leaves 360-379.
Electrification: an historical process -- A prehistory of electrification: the Sydney energy system to1881 -- Slow dawn of the electric light, 1881-1904 -- The momentum of growth, 1904-1932 -- The state takes charge, 1932-1950 -- Triumph of the grid, 1950-1986 -- The limits to electrification.
All technological systems require energy. The concentration of human population and economic activity in cities has relied on the development of urban energy systems, which bring energy to the city and distribute it to points of end use within it. Over the past century, electro-technology has come to dominate urban energy systems throughout the developed world. This process has been imperfectly documented and analysed, because the relationships between electricity and the energy service markets and local political frameworks within which each instance of urban electrificaiton has taken place have generally been neglected. -- This thesis presents electrification as an historical change in the urban energy system. It identifies the most important influences on urban energy demand and on the organisation of energy supply, and traces their interaction before the introduction of electro-technology, then from the beginning of electrification in the 1880s to its completion in the 1980s. -- Urban electrification is best observed and understood by following its course within a single city. Sydney is well suited to such an analysis, since it is highly electrified and encompasses within its two hundred year history all the major energy technologies of the past millenium. During the first century of its existence, it developed distinctively urban markets for transportation, street lighting, commercial, industrial and residential energy services. These were revolutionised by steam and by gas, the first specifically urban energy technology. -- The thesis examines how each energy form in turn gained a foothold in the Sydney energy system, diffused through it and spread beyond it to the rest of the state of New South Wales. It analyses long term trends in each of the various urban energy markets, and draws parallels in the pattern of succession of supply technologies. It demonstrates that these patterns were repeated with the introduction of electricity and, in the 1970s, by its emerging successors. -- During Sydney's second century each of its energy markets was electrified in turn, while its separate electricity supply systems coalesced into a unified grid serving the entire metropolis, and extending later into the rest of the state. Largely as a result of political circumstances in the 1880s, when electric lighting was first introduced, the municipal electricity supply organisations acquired considerable influence and autonomy, and resisted the later attempts of state governments to co-ordinate their development. --The electrification of the Sydney and NSW energy systems had largely run its course by the late 1970s. Electricity supply had exhausted the economies of scale and technological development which had given it an advantage over other fuels. It had saturated the urban energy markets, and was facing new competitors in the form of natural gas and more efficient utilisation technologies. These changes in the energy system exacerbated the inherent problems in the organisation of electricity supply, which was predicated on unlimited growth and slow to adapt to the end of electrification.
Mode of access: World Wide Web.
[13], 379 leaves ill., maps
Jayawickrema, Jacintha. "A reconstruction of the ecological history of Longneck Lagoon New South Wales, Australia /." View thesis, 2000. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20050720.135957/index.html.
Full textStenekes, Willem J. "History denied : a study of David Irving and Holocaust denial /." View thesis, 2002. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030704.164555/index.html.
Full text"A thesis presented to the University of Western Sydney in the fulfillment of the requirement for the degree of Master of Arts (Honours), May 2002." Bibliography: p. 300-333.
KARSKENS, Grace. "THE ROCKS AND SYDNEY: SOCIETY, CULTURE AND MATERIAL LIFE 1788-C1830." University of Sydney, History, 1995. http://hdl.handle.net/2123/405.
Full textBavinton, George M. "One man's vision : a play in two acts and an accompanying exegesis." Thesis, Queensland University of Technology, 2006. https://eprints.qut.edu.au/16311/2/George_Bavinton_-_One_Man%27s_Vision.pdf.
Full textBavinton, George M. "One man's vision : a play in two acts and an accompanying exegesis." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16311/.
Full textHughes, Lesley Patricia School of Social Work UNSW. "To labour seriously : Catholic sisters and social welfare in late nineteenth century Sydney." Awarded by:University of New South Wales. School of Social Work, 2002. http://handle.unsw.edu.au/1959.4/19047.
Full textHenderson, Peter Charles. "A history of the Australian extreme right since 1950 /." View thesis, 2002. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030924.134813/index.html.
Full text"A thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy, December 2002, School of Humanities, University of Western Sydney" Bibliography : p. [419]-451.
Nicholls, Philip Herschel. "A review of issues relating to the disposal of urban waste in Sydney, Melbourne and Adelaide : an environmental history." Title page, contents and abstract only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phn6153.pdf.
Full textBubacz, Beryl M. "The Female and Male Orphan Schools in New South Wales, 1801-1850." University of Sydney, 2007. http://hdl.handle.net/2123/2474.
Full textThis thesis is concerned with an examination and re-assessment of the establishment, operation and management of the Female and Male Orphan Schools, in the first half of the nineteenth century in New South Wales. The chaplains and governors in the early penal settlement were faced with a dilemma, as they beheld the number of children who were ‘orphaned’, neglected, abandoned and destitute. In order to understand the reasons why these children were in necessitous circumstances, the thesis seeks to examine the situations of the convict women, who were the mothers of these children. Governors Philip Gidley King and Lachlan Macquarie respectively in 1801 and 1819 established the Schools, which provided elementary education, training and residential care within a religious setting. Researching the motives underlying the actions of these men has been an important part of the thesis. An examination of the social backgrounds of some of the children admitted to these Schools has been undertaken, in order to provide a greater understanding of the conditions under which the children were living prior to their admissions. Information about family situations, and the social problems encountered by parents that led them to place their children in the Schools, have been explored. The avenues open to the girls and boys when they left the Schools, has formed part of the study. Some children were able to be reunited with family members, but the majority of them were apprenticed. A study of the nature of these apprenticeships, has led to a greater understanding of employment opportunities for girls and boys at that time. In 1850 the Schools were amalgamated into the Protestant Orphan School at Parramatta. By examining the governance and operation of the Schools during their last two decades as separate entities, we have more knowledge about and understanding of these two colonial institutions. It is the conclusion of this thesis that some of the harsher judgements of revisionist social historians need to be modified. It was the perception that more social disorder would occur if action was not taken to ‘rescue’ the ‘orphaned’ children, usually of convict parentage. However genuine charity, philanthropy and concern was displayed for the children in grave physical and moral danger. The goals of the founders were not always reached in the Orphan Schools, nevertheless they performed an invaluable service in the lives of many children.
Dance, Anne T. "Landscapes of perception : reclaiming the Athabasca oil sands and the Sydney tar ponds." Thesis, University of Stirling, 2013. http://hdl.handle.net/1893/16957.
Full textMcPherson, Ailsa School of Theatre Film & Dance UNSW. "Diversions in a tented field : theatricality and the images and perceptions of warfare in Sydney entertainments 1879-1902." Awarded by:University of New South Wales. School of Theatre, Film and Dance, 2001. http://handle.unsw.edu.au/1959.4/18264.
Full textTredinnick, Mark. "Writing the wild : place, prose & the ecological imagination /." View thesis, 2003. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20040630.093441/index.html.
Full textIuliano, Susanna. "Constructing Italian ethnicity : a comparative study of two Italian language newspapers in Australia and Canada, 1947-1957." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22595.
Full textThe specific focus of this study is the Italian language press and its attempts to shape the ideals of italianita of Italian migrants in Canada and Australia in the immediate post-war period. This work is based on two newspapers, Montreal's Il Cittadino Canadese and La Fiamma published in Sydney, New South Wales. All available editions from the decade 1947 to 1957 are examined in order to determine which symbols and causes were used to promote Italian ethnic cohesiveness.
In the course of this thesis, it is argued that La Fiamma used religion as the basis of its ideal of italianita, while the Italo-Canadian paper Il Cittadino Canadese made the issue of Italian political representation in Canadian government structures the basis of its quest to unite Italian migrants into an ethnic 'community'. Some possible reasons for the difference in focus between the two newspapers are presented in the conclusion. Also, suggestions are made for future comparative research between Italian ethnic communities in Canada and Australia which may help to better explain the differences laid bare in this paper.
Sawyer, Wayne. "Simply growth? : a study of selected episodes in the history of years 7-10 English in New South Wales from 1970s to the 1990s /." View thesis, 2002. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030623.111035/index.html.
Full textSchell, Christopher. "Ecology and life-history variation within a population of the frog Limnodynastes Tasmaniensis (Anura: Myobatrachidae) from a remnant woodland of the Cumberland plain in north-western Sydney /." View thesis, 2002. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20040423.154855/index.html.
Full text"A thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy at the University of Western Sydney, Centre for Integrated Catchment Management". References : leaves 133 - 162.
Farwig, Victoria Jane. "Evaluation of mineral magnetic properties and thermal activation characteristics of soil material in reconstructing post-fire sediment redistribution and fire history, Sydney Basin, Australia." Thesis, Swansea University, 2006. https://cronfa.swan.ac.uk/Record/cronfa43195.
Full textHallani, Houssein. "Utilising behaviour history and fuzzy trust levels to enhance security in ad-hoc networks." View thesis, 2007. http://handle.uws.edu.au:8081/1959.7/19135.
Full textA thesis submitted to the University of Western Sydney, College of Health and Science, School of Computing and Mathematics, in fulfilment of the requirements for the degree of Doctor of Philosophy. Includes bibliography.
Lloyd, Justine, University of Western Sydney, of Arts Education and Social Sciences College, and School of Cultural Histories and Futures. "I'd rather not be in Marrickville : aerial modernities and the domestication of the sublime." THESIS_CAESS_CHF_Lloyd _J.xml, 2000. http://handle.uws.edu.au:8081/1959.7/450.
Full textDoctor of Philosophy (PhD)
Frawley, J. W. "Country all round : the significance of a community's history for work and workplace education /." View thesis View thesis, 2001. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030416.131433/index.html.
Full text"A thesis submitted in the School of Applied Social and Health Sciences at the University of Western Sydney (Nepean) for the degree of Doctor of Philosophy, February 2001" Bibliography : leaves 327-343.
Norrie, Philip Anthony. "An Analysis of the Causes of Death in Darlinghurst Gaol 1867-1914 and the Fate of the Homeless in Nineteenth Century Sydney." University of Sydney, 2007. http://hdl.handle.net/2123/1862.
Full textThis thesis examines a ledger which listed all the causes of death in Darlinghurst Gaol, Sydney’s main gaol, from 1867 to 1914 when the gaol was closed and all the prisoners were transferred to the new Long Bay Gaol at Maroubra. The ledger lists the name of the deceased prisoner, the date of their death, the age of the prisoner at the time of their death and the cause of death along with any special comments relevant to the death where necessary. This ledger was analysed in depth and the death rates and diseases causing the deaths were compared to the general population in New South Wales and Australia as well as to another similar institution namely Auburn Prison, the oldest existing prison in New York State and the general population of the United States of America (where possible). Auburn Prison was chosen because it was the only other prison in the English speaking world (British Empire and United States of America) that had a similar complete list of deaths of prisoners in the same time frame – in this case beginning in 1888. The comparison showed that the highest death rates were in the general population of the United States of America (statistics on New York State alone could not be found) followed by Auburn Prison followed by the general population of Australia then the general population of New South Wales (the latter two were very similar) and the lowest death rates were in Darlinghurst Gaol. The analysis showed that individuals were less likely to die in the main prison, compared to the relevant general population in New South Wales and New York State despite the fact that 8 – 9% of these prison deaths were due to executions, a cause of death not encountered in the general population. This thesis explores the reasons why mortality rates were lower in prison despite the popular perception was that Victorian era gaols were places of harshness, cruelty and death (think of the writings of Charles Dickens, the great moralist writer who was the conscience of the era) compared to the general free population.
Schell, Christopher B., University of Western Sydney, of Science Technology and Environment College, and of Science Food and Horticulture School. "Ecology and life-history variation within a population of the frog Limnodynastes Tasmaniensis (Anura: Myobatrachidae) from a remnant woodland of the Cumberland plain in north-western Sydney." THESIS_CSTE_SFH_Schell_C.xml, 2002. http://handle.uws.edu.au:8081/1959.7/692.
Full textDoctor of Philosphy (PhD)
Garaty, Janice Royaline, and res cand@acu edu au. "Holy Cross College Woollahra 1908-2001: A micro-study of Catholic education in the Archdiocese of Sydney in the twentieth century." Australian Catholic University. School of Arts and Sciences, 2008. http://dlibrary.acu.edu.au/digitaltheses/public/adt-acuvp223.15102009.
Full textMayne, Patricia Anne. "A history of TAMAR (1996-2008) in relation to the Anglican Church of Australia in general and the Diocese of Sydney in particular. TAMAR (Towards A More Appropriate Response) was formed by a group of Sydney Anglican women to address the issue of sexual abuse in the Australian Anglican Church." Thesis, Australian Catholic University, 2016. https://acuresearchbank.acu.edu.au/download/eabcf422e231b2b679dae250ca2877917f8f111b144b5e0f343b2ca5a1e20c9c/35209611/Mayne_2016_A_history_of_tamar_in_relation_to.pdf.
Full textCork, Kevin James. "Twenty-four miles around Nelungaloo : the history and importance of cinema exhibition in pre-television times to a country area of central-western New South Wales /." View thesis, 1994. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030916.125146/index.html.
Full textBeder, Sharon Science & Technology Studies (STS) UNSW. "From pipe dreams to tunnel vision : engineering decision-making and Sydney's sewerage system." Awarded by:University of New South Wales. Science and Technology Studies (STS), 1989. http://handle.unsw.edu.au/1959.4/20621.
Full textRobinson, Raymond Stanley. "Dreaming tracks : history of the Aboriginal Islander Skills Development Scheme, 1972-1979 : its place in the continuum of Australian indigenous dance and the contribution of its African American founder, Carole Y. Johnson /." View thesis View thesis, 2000. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030604.085603/index.html.
Full textA thesis presented in fulfilment of the requirements for the degree of Master of Arts (Honours) - (Performance), School of Applied Social and Human Sciences, University of Western Sydney, Nepean, 2000. Bibliography : Vol. 1, leaves 202-209.
Cupido, Conroy Alan. "Significant influences in the composition of Hendrik Hofmeyr's song cycle, Alleenstryd." Thesis, connect to online resource, 2009. http://digital.library.unt.edu/ark:/67531/metadc12110.
Full textOrr, Kirsten School of Architecture UNSW. "A force for Federation: international exhibitions and the formation of Australian ethos (1851-1901)." Awarded by:University of New South Wales. School of Architecture, 2006. http://handle.unsw.edu.au/1959.4/23987.
Full textBlaser, Andrea. "Sustainability gap : a case study of Olympic development in Sydney, Australia and Beijing, China /." Connect to title online (Scholars' Bank) Connect to title online (ProQuest), 2008. http://hdl.handle.net/1794/8479.
Full textDumitrascu, Oana. "The Australian, The Age et The Sydney Morning Herald, trois grands quotidiens australiens vecteurs d'assimilation des Aborigènes? : Une étude de la période 1997-2007." Paris 9, 2012. https://portail.bu.dauphine.fr/fileviewer/index.php?doc=2012PA090027.
Full textThis thesis is a study of three major Australian newspapers (The Australian, The Age and The Sydney Morning Herald) over a ten year period. Focusing on three important issues for the relationship between Aborigines and non Aborigines (the stolen generations, land rights and reconciliation) this thesis analyses how the three newspapers present the Aborigines, what role they are being given in the nation by these newspapers and what image of the Aborigines do they build. This in order to find out if their techniques contribute to perpetrate a certain form of oppression of the Aborigines, and to what extent. Our research suggests that Australia's painful past regarding the encounter between Aborigines and non Aborigines, with the frontier wars, the dispossession and the stolen children, influences to different extents the way in which the three newspapers of our research write about Aborigines. The tendency to make their interest come last, to present them as enemies of the nation or as a threat to its stability, the tendency to imprison them in an image that has not changed since the 19th and 20th centuries and not give them the opportunity to express themselves on issues concerning them or their own image, are elements pointing to a certain form of oppression that stops Aborigines from taking an equal place to that of the other Australians in the contemporary Australian society. In this sense, this research contributes to the study of media and its ways of functioning as well as to the study of Australian history, society and politics
Brihault, Jean. "Lady Morgan et l'Irlande." Rennes 2, 1985. http://www.theses.fr/1985REN20016.
Full textDucrocq, Myriam-Isabelle. "Le réalisme dans la pensée politique anglaise de la Grande Rébellion à la Glorieuse Révolution : à travers les oeuvres de Thomas Hobbes, James Harrington, Algernon Sydney et John Locke." Paris 3, 2007. http://www.theses.fr/2007PA030153.
Full textThe purport of this doctoral thesis is to bring in perspective four emblematic thinkers of the period of English history extending between The Great Rebellion and the Glorious Revolution : Thomas Hobbes, John Harrington, Algernon Sydney and John Locke. These authors, whose respective status and posterity widely differ, are divided by strong ideological differences : Hobbes was a proponent of absolutist government, Harrington and Sidney inclined in favour of a regime based on popular representation and John Locke was a partisan of parliamentary monarchy. Whatever their differences, these philosophers share a common attitude that consists in taking into account the real conditions presiding over the exercise of political power. This common perspective which has been described as political realism is founded on three main axes : the first one is the will to choose a foundation of all political organisations the principles of nature as evidenced by the historical study of human societies and rational analysis ; the second axis is to be found in their search for optimal conditions to ensure the preservation of the Commonwealth built on such foundation ; the third and last axis consists in taking into account the Commonwealth ‘s interest in the resolution of a certain number of fundamental political questions that prevailed during that period. We shall establish in conclusion that their common realism induces them to put forward similar propositions on the nature and extent of the power that political leaders must be entrusted with, whatever the chosen form of government may be
Noordhuis-Fairfax, Sarina. "Field | Guide: John Berger and the diagrammatic exploration of place." Phd thesis, Canberra, ACT : The Australian National University, 2018. http://hdl.handle.net/1885/154278.
Full textThomas, Julian. "Heroic history and public spectacle : Sydney 1938." Phd thesis, 1991. http://hdl.handle.net/1885/112136.
Full text"History of the Sydney Film Festival 1954-1983." University of Technology, Sydney. Faculty of Humanities and Social Sciences, 2005. http://hdl.handle.net/2100/252.
Full textJohns, Leanne. "Entrepreneurs and their business networks in colonial Sydney, 1817-1824." Phd thesis, 2007. http://hdl.handle.net/1885/150145.
Full textBishop, Catherine Elizabeth. "Commerce was a woman : women in business in colonial Sydney and Wellington." Phd thesis, 2012. http://hdl.handle.net/1885/151294.
Full textHealy, Matthew. "Hard sell: Australian football in Sydney." Thesis, 2002. https://vuir.vu.edu.au/18171/.
Full textYingprasertchai, Thanvapon Senee. "Influence of metal exposure history on metal tolerance in the Sydney rock oyster (Saccostrea glomerata)." Thesis, 2016. http://hdl.handle.net/1959.13/1312288.
Full textTolerance to heavy metal pollution has been described in many living organisms exposed to heavy metals at sublethal levels. Organisms living in metal contaminated sites have to develop physiological processes to respond to metal exposure to ensure survival and reproduction. The organisms may respond to this challenge through physiological acclimation or adaptation (the selection of resistant genotypes). This thesis was focused on the influence of prior metal exposure history on metal tolerance in Sydney rock oyster, Saccostrea glomerata in NSW, Australia. The thesis explored both physiological acclimation and possible adaptation to metal stress. To determine the level of metal contamination among estuaries in NSW and indicate the most relevant metals for further experimental studies, multivariate statistical techniques such as principle component analysis (PCA) and factor analysis (FA), and ecological risk indices including contamination factor (Cf), degree of contamination (Cd) and pollution loading index (PLI) were employed. Using accumulated metals in tissues of the oysters growing wild and farmed in NSW, these analyses identified locations both low and elevated in metals and identified metals most responsible for driving differences among locations.. The results indicated that most of polluted estuaries were located in the Central region of NSW, having impacts from large urban and industrial conurbation, while the unpolluted estuaries were located in the Northern / Southern region within the major regional waterways with low urban activity. The most elevated metals among NSW estuaries were cadmium, copper, lead and zinc. To investigate possible adaptation, the influence of metal exposure history on metal tolerance in the Sydney rock oyster (Saccostrea glomerata) offspring was investigated. The oysters were sampled from ten NSW estuaries such as Clyde River, Hastings River, Hunter River, Manning River, Nambucca, North Haven, Port Stephens, Port Kembla, Swansea Channels, and Wallis Lake which have a gradient in metal exposure history based on the results of multivariate analysis (PCA/FA) and ecological indices (Cf, Cd and PLI) of metals accumulated to adult oysters. Embryos were prepared under laboratory conditions by fertilizing sperm from ten males and oocytes from ten females within estuary using a strip spawning technique. The embryos were then exposed to sublethal concentrations of 2.5-40 μg/l, copper and 7.5-120 μg/l zinc for 48 h. The median effective concentration (EC50) was determined as the metal concentration that caused 50% abnormal D-veliger larvae development.. The EC50 values for copper were not significantly different among estuaries. Interestingly, a strong positive correlation between EC50 and tissue metal concentration was found for zinc (R² = 0.835, p<0.000), but not for copper. Locations with higher environmental Zn exposures had more tolerant offspring. Such a finding suggests adaptation to Zn exposure (the selection of resistant genotypes) or a physiological acclimation effect mediated via maternal transfer. The full-length genomic sequence of S. glomerata, metallothionein (sgMT) was cloned and characterized. The sgMT sequence was composed of two metal responsive elements (MRE), one TATA box, one activator protein-1 (AP-1), three coding exons (28, 117, 80 bp), and two introns (115, 393 bp). The coding exons encoding a protein of 74 amino acids, with 9 cysteine motifs (Cys-X-Cys), cysteine-rich (28%), high lysine content (13.5%) and no aromatic amino acid residues. The phylogenetic analysis of sgMT protein sequence revealed that the sgMT was classified into the MT isoform I. Sequencing of full length of MT mRNA and MT genomic DNA allowed the identification of an intron-exon boundary and the development of the quantitative real time polymerase chain reaction (qRT-PCR). The qRT-PCR revealed that tissue-specific MT gene expression in S. glomerata was expressed highest in the digestive gland; significantly higher than gills, mantle, adductor muscle and gonad. The digestive gland was selected as a target tissue for a further examination of S. glomerata MT gene expression. Physiological acclimation to metals was also investigated by comparing adult oysters with a past history of metal exposure to oysters with no past history of metal exposure (both from the same gene pool) and examining their responses to subsequent metal challenges. Specifically, the experiment involved examining the influence of prior metal exposure history in wild Sydney rock oyster (Saccostrea glomerata) on metal accumulation, MT mRNA expression, glutathione peroxidase (GPx) and superoxide dismutase (SOD) upon subsequent cadmium, copper and zinc exposure. Oysters were sampled from seven locations in Hunter River such as Fullerton Cove, Kooragang Dykes, Windmill, South Arm Bridge, Fern bay, Fullerton St Stockton and Stockton Bridge. The results of multivariate analysis (PCA/FA) and ecological indices (Cf and Cd) of metal accumulation in the oyster tissue from seven locations elucidated that Windmill was the most contaminated with metals, while Fern Bay was the lowest. Copper and zinc were the most elevated metals with 3.71 and 2.51 times higher than NSW background, thus these metals were selected as metal candidates for metal exposure experiment. Cadmium was included as a positive control. Windmill(high past metal exposure history) and Fern Bay( negligible past metal exposure history)oysters were exposed to 20 and 200 μg/l of cadmium, 50 and 500 μg/l of copper and 200 and 2000 μg/l of zinc for 14 days. After exposure, MT mRNA expression, GPx and SOD activities were measured in digestive glands. The two-way ANOVA results revealed that Windmill oysters activated MT mRNA expression significantly higher than Fern bay oysters after exposure to 200 μg/l Cd, F(2, 22) = 10.35, p = 0.0007 and 2000 μg/l Zn, F(2, 21) = 24.28, p < 0.0001. The GPx activity was not significantly different between those oysters. The SOD activity showed significant interaction effects for copper with F(2, 21) = 10.91, p = 0.0006, indicating that Windmill oysters produced SOD activity significantly higher than Fern bay oysters after 50 μg/l Cu exposure. Significant interaction was also found for zinc with F(2, 24) = 7.27, p = 0.0034, where Fern bay oysters produced SOD higher than Windmill oysters after 200 μg/l Zn exposure, while Windmill oysters produced SOD higher than Fern bay oysters after 2000 μg/l Zn exposure. Thus prior exposure to metals can result in an upregulated compensatory response upon subsequent exposure to metals indicative of acclimation. Both acclimation, and potentially adaptation, are mechanisms responsible for the observed tolerance to metals in the Sydney Rock Oyster.
Swanton, Bruce. "Protecting Sydney, 1788-1862 : public security in a colonial environment." Master's thesis, 1996. http://hdl.handle.net/1885/145954.
Full textHely, Patsy. "Clay objects and the articulation of place." Phd thesis, 2007. http://hdl.handle.net/1885/151498.
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