Academic literature on the topic 'Surfce anlaysis'

Abstract Introduction Notch signaling is essential for the optimal generation of T, B and myeloid cells. Epidermal growth factor-like (EGF) repeats in the extracellular domain of Notch receptors are modified by O-fucose and O-GlcNAc glycans transferred by protein O-fucosyltransferase 1 (POFUT1) and EGF O-GlcNAc-transferase (EOGT), respectively. EOGT promotes Notch ligand binding and Notch signaling in the developing retina in mice, and its loss is the basis of Adam's Oliver Syndrome 4 (AOS4) in humans. The loss of POFUT1 in humans leads to the Dowling Degos Disease 2 (DDD2). Thus, O-fucose and O-GlcNAc glycans may have different functions in Notch signaling, reflected in different requirements for T, B or myeloid cell development. Methods: Eogt control, Eogt null, Pofut1 floxed and Vav1-iCre transgenic mice were used to generate single and compound mutant mice with inactive Eogt, Pofut1 or both Eogt and Pofut1. Antibody markers of hematopoietic stem progenitor cell (HSPC), lymphoid and myeloid subsets were used to identify different T, B and myeloid cell subsets by flow cytometry using the Cytek TM Aurora Flow Cytometer. FCS files were analyzed using FlowJo software (BD). CD45.1+ (B6.SJL-Ptprc a Pepc b/BoyJ #002014) congenic mice were used for bone marrow transfer. Expression of NOTCH1 and binding of soluble Notch ligands to DN T cells was analyzed by flow cytometry. Expression of different Notch target genes was determined using qRT-PCR anlaysis. Results: Eogt null mice exhibited altered production of certain T cell subsets in thymus and B cell subsets in spleen, most similar to alterations observed in mice lacking LFNG, MFNG and RFNG. This phenotype was cell-autonomous as Eogt null bone marrow cells transferred defective T and B cell development to irradiated recipient mice. More severe defects in hematopoiesis were observed in mice conditionally lacking Pofut1 in hematopoietic stem cells (HSC) via Vav1-iCre. However , deletion of both O-fucose and O-GlcNAc glycans together in Eogt:Pofut1 double knockout HSC, led to the most severe decrease in common lymphoid and myeloid progenitors in bone marrow. In thymus, the dramatic reduction in T cell subsets observed in Pofut1 cKO mice was reduced further (in DN2 subsets) in Eogt:Pofut1 dKO mice. In spleen, there was a significantly greater decrease in follicular B and other B cell populations, and a greater increase in CD11b/c+ and Gr1+ myeloid cells in Eogt:Pofut1 dKO mice. Splenomegaly occurred in both Pofut1 cKO and Eogt:Pofut1 dKO mice, with a greater increase in extramedullary hematopoiesis in the dKO spleen. Binding of soluble DLL4-Fc was >90% reduced in Pofut1 cKO DN T cells, and not further reduced in dKO cells, while NOTCH1 expression at the cell surface was only slightly reduced. The relative expression of Notch target genes including Hes1, CD25, cMyc, and Deltex1 was similarly reduced in Pofut1 cKO and Eogt:Pofut1 dKO DN T cell progenitors. Conclsuion: The combined data suggest that O-fucose and O-GlcNAc glycans act in an additive fashion to optimize Notch signaling in lymphoid and myeloid differentiation. Keywords: Notch Signaling, O-Glycans, Protein O-fucosyltransferase 1 (POFUT1), O-GlcNAc transferase (EOGT), Lymphopoiesis, Myelopoiesis. Disclosures Stanley: Aviceda Therapeutics, Inc.: Consultancy, Current holder of stock options in a privately-held company.

Bioemulsifier production from a Probiotic Lactobacillus plantarum isolated from a rice basedfermented product was studied. The optimization of production of bio emulsifier as a function offermentation time in shaker and stationary conditions was carried out. The highest production of bioemulsifier with 38.63% decline in surface tension at 72 hoccurred under stationary conditions. The isolatedbio emulsifier is partially purified for characterization and anlaysis of CMC (0.45 g l1), oil displacement area,emulsification index, emulsifying activity and emulsion stability. Results showed that the bio emulsifier hadgood emulsifying property and FTIR analysis indicates the presence of proteins and polysaccharidesfractions. Aqueous solutions of the bioemulsifier displayed antimicrobial activity and antiadhesive activityagainst the pathogens: Escherichia coli ATCC, Escherichia coli ETCC, Yersinia enterocolitica and Staphylococcusaureus. All the results indicate that the bioemulsifier produced from L. plantarum can be utilized in foodformulation as a multiuse food additive.

AbstractThe climate of West Africa is expected to become more arid due to increased temperature and uncertain rainfall regimes, while its population is expected to grow faster than the rest of the world. As such, increased demand for food will likely coincide with declines in agricultural production in a region where severe undernutrition already occurs. Here, we attempt to discriminate between the impacts of climate and other factors (e.g. land management/degradation) on crop production across West Africa using satellite remote sensing. We identify trends in the land surface phenology and climate of West African croplands between 2000 and 2018. Using the combination of a an attribution framework and residual trend anlaysis, we discriminate between climate and other impacts on crop productivity. The combined effect of rainfall, land surface temperature and solar radiation explains approximately 40% of the variation in cropland productivity over West Africa at the 95% significance level. The largest proportions of croplands with greening trends were observed in Mali, Niger and Burkina Faso, and the largest proportions with browning trends were in Nigeria, The Gambia and Benin. Climate was responsible for 52% of the greening trends and 25% of the browning trends. Within the other driving factors, changes in phenology explained 18% of the greening and 37% of the browning trends across the region, the use of inputs and irrigation explained 30% of the greening trends and land degradation 38% of the browning trends. These findings have implications for adaptation policies as we map out areas in need of improved land management practices and those where it has proven to be successful.

A total number of 201 surface soil samples were collected encompassing the fields of Central Farm of Horticultural College and Research Institute, Periyakulam, Tamil Nadu. The GPS data (Latitude ºN and Longitude ºE) were recorded for each sampling site by using GPS-Garmin eTrex Vista HCX model. Field maps were collected and field number wise digitization was done. Soil samples were processed and anlaysed for soil physic-chemical and fertility parameters. Results indicated that soil samples were neutral to alkaline in reaction, non saline, and slightly calcareous to non calcareous in nature. Soil fertility groupings under percent category indicated that the soils were medium in soil organic carbon, low in available nitrogen, medium to high in available phosphorus, medium to high in available potassium, and low in available sulphur. With respect to DTPA extractable micronutrients, Fe, Zn, Mn and Cu were found to dominate by low to medium, low, high, medium to high categories, respectively. HWS-Boron was also recorded under the high category. The nutrient index values of the samples indicated a high status for organic carbon, available P and K, while medium for available N and adequate for available sulphur. With respect to micro nutrients, nutrient index values indicated that adequate for DTPA-Zn and very high for DTPA-Fe, Mn, Cu, and HWS-B. Thematic maps generated on the individual parameters depicted the spatial variability of parameters in the Central Farm of Horticultural College and Research Institute, Periyakulam. In the identified areas of poor fertility status, nutrient deficiency has to be eliminated by the application of organic and /or inorganic sources to maintain sustainable soil fertility status. Soil test-based fertilizer recommendations and micronutrients are to be followed to mitigate nutrient deficiencies and achieve sustained crop production and soil fertility.

Electromagnetic wave scattering from a semi-infinite array of dipoles in free space is described by using asymptotic high frequency methods. An electric field integral expression is obtained and solved with asymptotic high frequency methods. An asymptotic field expression is obtained for a finite ×
infinite array of dipoles in free space. The analytical closed form expression for the array guided surface wave launching coefficient is obtained via a combination of an asymptotic high frequency analysis of a related reciprocal problem and Lorentz reciprocity integral formulation for the semi-infinite planar dipole array in which modified Kirchhoff approximation is used. The accuracy and the validity of the asymptotic analytical solutions are compared with the numerical solutions available in the literature before.

TiO2 films were deposited by the sol–gel dip coating method assisted by ultra sonic agitation, for their use as propane gas sensors. XRD anlaysis revealed the TiO2 anatase phase for all samples. The film thickness was controlled with the number of the dip-coating immersion cycles, obtaining thicknesses from 10 to 130 nm and a growing rate of 18 nm per immersion. Surface morphology shows that films grow more compact and densely packed as the number of immersion cycles increase. Gas sensing studies indicate that work temperatures above 300°C are needed for sensing activation of the films. Gas sensors sensitivity as a function of propane concentration and film thickness revealed a two-regime behavior: for partial pressures of propane above 400 ppm the sensitivity is not dependent of the film thickness, whereas it is dependent on this parameter at lower partial pressures. Such behavior is attributed to a space charge region and a change in surface structure. For the low concentration regime, the maximum sensitivity was obtained for a film thickness of 110 nm or 5 inmersions.