Journal articles on the topic 'Sucrose Synthesis'

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1

Bhuvaneswari, E., B. Sailaja, and S. Sivaprasad. "Impact of photoperiod on circadian sucrose and sucrase rhythms in the digestive system of silkworm, Bombyx mori." Journal of Applied and Natural Science 5, no. 1 (June 1, 2013): 230–41. http://dx.doi.org/10.31018/jans.v5i1.312.

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The impact of photoperiod on circadian sucrose and sucrase rhythms were analyzed in the digestive system of Bombyx mori under 12 hr light-dark cycle (LD), continuous light (LL) and continuous dark (DD). The rhythmic changes were interpreted as synthetic cycles in gut wall and release or uptake cycles in gut lumen. The gut wall comprised 6 sucrose synthetic cycles (SS cycles) under LD, LL and 5 under DD. The 24 hr rhythm of LD and LL was clock shifted to 28.8 hr under DD. In gut content, the sucrose rhythm showed 7 sucrose uptake cycles (SUcycles) under LD, 6 under LL and 5 under DD and the 24 hr rhythm of LD was clock shifted to 28.0 hr under LL and 34 hr under DD. In the gut wall sucrase rhythm maintained 7 SES cycles under LD and DD and 9 cycles under LL and its 24-hr rhythm is advanced to 18.2 hr. In the gut lumen 5 SER cycles under LD, 8 under LL and 6 under DD and its rhythm is advanced to 15 hr under LL and 20 hr under DD. Further analysis of data showed that LD favoured both synthesis and uptake of sucrose while LL, favoured the sucrase synthesis and its release.
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2

Dyer, Ulrich C., and Yoshito Kishi. "Synthesis of C-sucrose." Journal of Organic Chemistry 53, no. 14 (July 1988): 3383–84. http://dx.doi.org/10.1021/jo00249a056.

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3

Lunn, John Edward. "Evolution of Sucrose Synthesis." Plant Physiology 128, no. 4 (April 1, 2002): 1490–500. http://dx.doi.org/10.1104/pp.010898.

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4

Suzuki, Michio, and Christopher J. Pollock. "Extraction and characterization of the enzymes of fructan biosynthesis in timothy (Phleum pratense)." Canadian Journal of Botany 64, no. 9 (September 1, 1986): 1884–87. http://dx.doi.org/10.1139/b86-250.

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A preparation of phlein sucrase from seedling shoots of timothy (Phleum pratense L.) is described which catalyzed the synthesis of fructan with a mean molecular size of 34 000 using sucrose as the substrate. Activity was fully sedimentable at 25 000 × g, had a pH optimum of 7.0, and a Km for sucrose of 0.15 M. Activity was inhibited by β-mercaptoethanol and sodium diethyl dithiocarbamate. Raffinose and stachyose, but not members of the kestose series of oligofructans, could act as fructosyl donors in addition to sucrose. Formation of oligosaccharides during high molecular weight fructan synthesis was minimal, with synthesis occurring by a mechanism apparently analogous to bacterial levansucrase. These observations are discussed in relation to the in vivo patterns of fructan biosynthesis observed in different species of higher plants.
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5

Nakai, Tomonori, Naoto Tonouchi, Takayasu Tsuchida, Hitoshi Mori, Fukumi Sakai, and Takahisa Hayashi. "Synthesis of Asymmetrically Labeled Sucrose by a Recombinant Sucrose Synthase." Bioscience, Biotechnology, and Biochemistry 61, no. 11 (January 1997): 1955–56. http://dx.doi.org/10.1271/bbb.61.1955.

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6

Lay, Luigi, Francesco Nicotra, Cristina Pangrazio, Luigi Panza, and Giovanni Russo. "Synthesis of antimetabolites of sucrose." Journal of the Chemical Society, Perkin Transactions 1, no. 3 (1994): 333. http://dx.doi.org/10.1039/p19940000333.

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7

Song, Zi-juan, Shu-jun Li, Xi Chen, Li-mei Liu, and Zhan-qian Song. "Synthesis of insecticidal sucrose esters." Forestry Studies in China 8, no. 3 (September 2006): 26–29. http://dx.doi.org/10.1007/s11632-006-0019-2.

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8

Hisajima, S., Y. Arai, and T. A. Thorpe. "Sucrose synthesis in callus cultures." Biologia Plantarum 27, no. 1 (January 1985): 74–77. http://dx.doi.org/10.1007/bf02894639.

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9

Ehira, Shigeki, Satoshi Kimura, Shogo Miyazaki, and Masayuki Ohmori. "Sucrose Synthesis in the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain PCC 7120 Is Controlled by the Two-Component Response Regulator OrrA." Applied and Environmental Microbiology 80, no. 18 (July 7, 2014): 5672–79. http://dx.doi.org/10.1128/aem.01501-14.

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ABSTRACTThe filamentous, nitrogen-fixing cyanobacteriumAnabaenasp. strain PCC 7120 accumulates sucrose as a compatible solute against salt stress. Sucrose-phosphate synthase activity, which is responsible for the sucrose synthesis, is increased by salt stress, but the mechanism underlying the regulation of sucrose synthesis remains unknown. In the present study, a response regulator, OrrA, was shown to control sucrose synthesis. Expression ofspsA, which encodes a sucrose-phosphate synthase, andsusAandsusB, which encode sucrose synthases, was induced by salt stress. In theorrAdisruptant, salt induction of these genes was completely abolished. The cellular sucrose level of theorrAdisruptant was reduced to 40% of that in the wild type under salt stress conditions. Moreover, overexpression oforrAresulted in enhanced expression ofspsA,susA, andsusB, followed by accumulation of sucrose, without the addition of NaCl. We also found that SigB2, a group 2 sigma factor of RNA polymerase, regulated the early response to salt stress under the control of OrrA. It is concluded that OrrA controls sucrose synthesis in collaboration with SigB2.
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10

Szyszka, Łukasz, Piotr Cmoch, Aleksandra Butkiewicz, Mykhaylo A. Potopnyk, and Sławomir Jarosz. "Synthesis of Cyclotriveratrylene-Sucrose-Based Capsules." Organic Letters 21, no. 16 (August 7, 2019): 6523–28. http://dx.doi.org/10.1021/acs.orglett.9b02451.

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11

TAKAHASHI, Hideki, Kozo HARA, Hitoshi HASHIMOTO, Takashi SASAKI, and Hajime TANIGUCHI. "Synthesis of Glucosylxyluloside Using Sucrose Phosphorylase." Journal of the Japanese Society of Starch Science 40, no. 1 (1993): 1–5. http://dx.doi.org/10.5458/jag1972.40.1.

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12

Khan, Riaz, Michael R. Jenner, Harold Lindseth, Khizar S. Mufti, and Gita Patel. "Ring-opening reactions of sucrose epoxides: Synthesis of 4′-derivatives of sucrose." Carbohydrate Research 162, no. 2 (May 1987): 199–207. http://dx.doi.org/10.1016/0008-6215(87)80215-x.

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13

Towill, Leslie R. "Mobilisation of carbohydrates during germination of spores of Onoclea sensibilis L." Proceedings of the Royal Society of Edinburgh. Section B. Biological Sciences 86 (1985): 203–12. http://dx.doi.org/10.1017/s0269727000008149.

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SynopsisA high percentage of germination of Onoclea sensibilis L. spores is initiated by a low fluence of red light while imbibed but unirradiated spores exhibit a very low percentage of germination. Onoclea spores contain lipid, protein and sucrose reserves. Sucrose is degraded and starch is synthesised during germination while lipid reserves are mobilised during early gametophytic development. The amount of protein remains constant during both developmental stages. There is very little detectable mobilisation of any of the reserves in the imbibed but ungerminating spores maintained in the dark for up to 18 days. Sucrose degradation and starch synthesis are not interrelated because photoenhanced sucrose degradation can occur without starch synthesis and photoinduction of starch synthesis can occur without sucrose degradation. Evidence is presented to suggest that the mode of action of light in enhancing starch synthesis is to increase the availability of a starch precursor rather than the activities of starch synthesising enzymes. Sucrose may be mobilised after irradiation because it becomes accessible to the sucrose degrading enzymes which are in abundance in the spores.
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14

Sun, Shijing, Zhongyuan Zhao, and Kenji Umemura. "Further Exploration of Sucrose-Citric Acid Adhesive: Synthesis and Application on Plywood." Polymers 11, no. 11 (November 13, 2019): 1875. http://dx.doi.org/10.3390/polym11111875.

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The development of eco-friendly adhesives is a major research direction in the wood-based material industry. Previous research has already demonstrated the mixture of sucrose and citric acid could be utilized as an adhesive for the manufacture of particleboard. Herein, based on the chemical characteristics of sucrose, a synthesized sucrose-citric acid (SC) adhesive was prepared, featuring suitable viscosity and high solid content. The investigation of synthesis conditions on the bond performance showed that the optimal mass proportion between sucrose and citric acid was 25/75, the synthesis temperature was 100 °C, and the synthesis time was 2 h. The wet shear strength of the plywood bonded with SC adhesive, which was synthesized at optimal conditions and satisfied the China National Standard GB/T 9846-2015. The synthesis mechanism was studied by both 13C NMR analysis and HPLC, and the chemical composition manifesting caramelization reaction occurred during the synthesis process. The results of ATR FT-IR indicated the formation of a furan ring, carbonyl, and ether groups in the cured insoluble matter of the SC adhesive, which indicated dehydration condensation as the reaction mechanism between sucrose and citric acid.
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15

Morrill, J. S., L. K. Kwong, P. Sunshine, G. M. Briggs, R. O. Castillo, and K. K. Tsuboi. "Dietary CHO and stimulation of carbohydrases along villus column of fasted rat jejunum." American Journal of Physiology-Gastrointestinal and Liver Physiology 256, no. 1 (January 1, 1989): G158—G165. http://dx.doi.org/10.1152/ajpgi.1989.256.1.g158.

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Adult rats when fed a high carbohydrate diet of 70% sucrose or glucose for 24 h following a 4-day fast showed increased concentrations of intestinal sucrase-isomaltase (EC 3.2.1.48, EC 3.2.1.10) and maltase-glucoamylase (EC 3.2.1.20) but not lactase-phlorizin hydrolase (EC 3.2.1.23, EC 3.2.1.62). The concentration increases of these enzymes were accompanied by corresponding acceleration of their synthesis rates. Contrary to earlier studies by others, suggesting that upper villus cells in the fasted intestine are unresponsive to stimulation of sucrase activity by refeeding a high-sucrose diet, the concentration increases of both sucrase-isomaltase and maltase-glucoamylase were seen to occur in cells all along the length of the villus column. The earlier studies differed from the present study by basing enzyme assays relative to protein rather than the DNA content of villus cell fractions. We have shown that villus cells increase their protein content severalfold while migrating to villus tip, providing the basis for the difference between earlier and the present findings. Further evidence that stimulation of sucrase-isomaltase and maltase-glucoamylase by high carbohydrate is not restricted to the crypt and lower villus region was obtained by the finding that their synthesis rates appeared to be equally stimulated along the length of the villus column.
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16

Sheiham, Aubrey. "Sucrose and Dental Caries." Nutrition and Health 5, no. 1-2 (April 1987): 25–29. http://dx.doi.org/10.1177/026010608700500205.

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Sucrose is unequivocally implicated in the cause of dental caries. Biochemical, microbiological, animal and human clinical and epidemiological evidence support a causal relationship. The risk of caries is related both to the amount and the frequency of intake of sucrose. The evidence that sucrose is important is that a) extracellular synthesis of polysaccharides by plaque bacteria is dependent on high concentration of sucrose. Without synthesis of polymers S. mutans cannot colonize the mouth in large numbers, b) studies on animals show a relationship between sucrose content of a food and its cariogenicity, c) there is a direct relationship between the quantity of sucrose consumed and caries in humans, d) the relationship between dietary sucrose and caries in humans approximates an S-shaped curve that rises more steeply when the sucrose-containing products are consumed frequently and when newly erupted teeth are present in young children and adolescents. Following the sharp rise, the curve flattens out. Sucrose is much more cariogenic than starch in humans. Reduction in sucrose consumption levels by half will benefit dental health and is unlikely to have any detrimental effects on health.
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17

Zheng, Yi, Spencer Anderson, Yanfeng Zhang, and R. Michael Garavito. "The Structure of Sucrose Synthase-1 from Arabidopsis thaliana and Its Functional Implications." Journal of Biological Chemistry 286, no. 41 (August 24, 2011): 36108–18. http://dx.doi.org/10.1074/jbc.m111.275974.

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Sucrose transport is the central system for the allocation of carbon resources in vascular plants. During growth and development, plants control carbon distribution by coordinating sites of sucrose synthesis and cleavage in different plant organs and different cellular locations. Sucrose synthase, which reversibly catalyzes sucrose synthesis and cleavage, provides a direct and reversible means to regulate sucrose flux. Depending on the metabolic environment, sucrose synthase alters its cellular location to participate in cellulose, callose, and starch biosynthesis through its interactions with membranes, organelles, and cytoskeletal actin. The x-ray crystal structure of sucrose synthase isoform 1 from Arabidopsis thaliana (AtSus1) has been determined as a complex with UDP-glucose and as a complex with UDP and fructose, at 2.8- and 2.85-Å resolutions, respectively. The AtSus1 structure provides insights into sucrose catalysis and cleavage, as well as the regulation of sucrose synthase and its interactions with cellular targets.
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18

Mach, Mateusz, and Sławomir Jarosz. "REACTION OF SUGAR PHOSPHONATES WITH SUCROSE ALDEHYDES. SYNTHESIS OF HIGHER ANALOGS OF SUCROSE." Journal of Carbohydrate Chemistry 20, no. 5 (June 30, 2001): 411–24. http://dx.doi.org/10.1081/car-100105713.

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19

Sun, Shijing, Min Zhang, Kenji Umemura, and Zhongyuan Zhao. "Investigation and Characterization of Synthesis Conditions on Sucrose-ammonium Dihydrogen Phosphate (SADP) Adhesive: Bond Performance and Chemical Transformation." Materials 12, no. 24 (December 6, 2019): 4078. http://dx.doi.org/10.3390/ma12244078.

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Sucrose is one of the most abundantly available renewable chemicals in the world, and it is expected to be utilized as a raw material for wood-based material products. Herein, a novel adhesion system that was based on sucrose and ammonium dihydrogen phosphate (ADP) was synthesized into an adhesive with 80% solid content, and this eco-friendly was utilized on the fabrication of plywood. The effects of the synthesis conditions on the plywood bond performance and synthesis mechanism were investigated. The optimal synthesis conditions were as follows: the mass proportion between sucrose and ADP was 90/10, the synthesis temperature was 90 °C, and the synthesis time was 3 h. The bonding performance of the plywood that was bonded by optimal SADP adhesive satisfied the GB/T 9846-2015 standard. The chemical analysis was performance tested by using High-Performance Liquid Chromatography (HPLC), Attenuated Total Reflection-Fourier Transform Infrared Spectra (ATR-FTIR), and Pyrolysis Gas Chromatography and Mass Spectrometry (Py-GC/MS) to understand the chemical transformation during the synthesis process. The chemical analysis results confirmed that the hydrolysis and conversation reaction of sucrose occurred in the synthesized SADP adhesive, and ADP promoted the pyrolysis efficiency of sucrose.
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20

Jadhav, Jagruti, and Amit P. Pratap. "Enzymatic Synthesis and Characterization of Sucrose Erucate." Tenside Surfactants Detergents 54, no. 6 (November 15, 2017): 539–45. http://dx.doi.org/10.3139/113.110528.

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21

Sokołowska, Patrycja, Michał Kowalski, and Sławomir Jarosz. "First synthesis of cryptands with sucrose scaffold." Beilstein Journal of Organic Chemistry 15 (January 23, 2019): 210–17. http://dx.doi.org/10.3762/bjoc.15.20.

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22

Vlahov, Iontcho, Petinka Vlahova, and Robert Linhardt. "Regioselective Synthesis of Sucrose Monoesters as Surfactants." Journal of Carbohydrate Chemistry 16, no. 1 (January 1, 1997): 1–10. http://dx.doi.org/10.1080/07328309708006506.

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23

Uenishi, Jun’ichi, and Atsushi Ueda. "Synthesis of (+)-sucrose via β-d-psicofuranosylation." Tetrahedron: Asymmetry 19, no. 18 (September 2008): 2210–17. http://dx.doi.org/10.1016/j.tetasy.2008.09.008.

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24

Lunn, John E., and Elspeth MacRae. "New complexities in the synthesis of sucrose." Current Opinion in Plant Biology 6, no. 3 (June 2003): 208–14. http://dx.doi.org/10.1016/s1369-5266(03)00033-5.

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25

Chen, J., and K. Park. "Synthesis of fast-swelling, superporous sucrose hydrogels." Carbohydrate Polymers 41, no. 3 (March 2000): 259–68. http://dx.doi.org/10.1016/s0144-8617(99)00144-7.

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26

Barrett, Anthony G. M., Barend C. B. Bezuidenhoudt, and Laura M. Melcher. "Redox glycosidation: a stereoselective synthesis of sucrose." Journal of Organic Chemistry 55, no. 18 (August 1990): 5196–97. http://dx.doi.org/10.1021/jo00305a008.

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27

Patil, Damodar R., Jonathan S. Dordick, and David G. Rethwisch. "Chemoenzymatic synthesis of novel sucrose-containing polymers." Macromolecules 24, no. 11 (May 1991): 3462–63. http://dx.doi.org/10.1021/ma00011a068.

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28

LAY, L., F. NICOTRA, C. PANGRAZIO, L. PANZA, and G. RUSSO. "ChemInform Abstract: Synthesis of Antimetabolites of Sucrose." ChemInform 25, no. 23 (August 19, 2010): no. http://dx.doi.org/10.1002/chin.199423218.

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29

Park, Hyun Gyu, Ho Nam Chang, and Jonathan S. Dordick. "Chemoenzymatic synthesis of sucrose-containing aromatic polymers." Biotechnology and Bioengineering 72, no. 5 (2001): 541–47. http://dx.doi.org/10.1002/1097-0290(20010305)72:5<541::aid-bit1018>3.0.co;2-c.

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30

Baik, Youngmin, Kartik Shanker, Joseph R. McDermid, and Robin A. L. Drew. "Carbothermal Synthesis of Aluminum Nitride Using Sucrose." Journal of the American Ceramic Society 77, no. 8 (August 1994): 2165–72. http://dx.doi.org/10.1111/j.1151-2916.1994.tb07113.x.

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31

Wernicke, Angelika, Stanislaw Belniak, Sophie Thévenet, Gérard Descotes, Alain Bouchu, and Yves Queneau. "Synthesis of sucrose carbonates in aqueous medium." Journal of the Chemical Society, Perkin Transactions 1, no. 7 (1998): 1179–82. http://dx.doi.org/10.1039/a800096d.

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32

Potopnyk, Mykhaylo A., Piotr Cmoch, and Sławomir Jarosz. "Short Synthesis of Diamide-Linked Sucrose Macrocycles." Organic Letters 14, no. 16 (August 2, 2012): 4258–61. http://dx.doi.org/10.1021/ol301993d.

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33

Strumia, M. C., M. N. Zamora, and H. E. Bertorello. "Hydrogels from acrylic sucrose. Synthesis and characterization." Journal of Applied Polymer Science 49 (1991): 9–14. http://dx.doi.org/10.1002/app.1991.070490003.

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34

Kim, K. B., and E. J. Behrman. "A new synthesis of sucrose 6′-phosphate." Carbohydrate Research 277, no. 2 (November 1995): C9. http://dx.doi.org/10.1016/0008-6215(95)00267-w.

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35

Kim, K. B., and E. J. Behrman. "A new synthesis of sucrose 6′-phosphate." Carbohydrate Research 270, no. 1 (April 1995): 71–75. http://dx.doi.org/10.1016/0008-6215(94)00003-x.

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36

Cruces, M. Angeles, Francisco J. Plou, Manuel Ferrer, Manuel Bernabé, and Antonio Ballesteros. "Improved synthesis of sucrose fatty acid monoesters." Journal of the American Oil Chemists' Society 78, no. 5 (May 2001): 541–46. http://dx.doi.org/10.1007/s11746-001-0300-5.

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37

Hill, L. M., and S. Rawsthorne. "Carbon supply for storage-product synthesis in developing seeds of oilseed rape." Biochemical Society Transactions 28, no. 6 (December 1, 2000): 667–69. http://dx.doi.org/10.1042/bst0280667.

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The aim of this work was to find out how the sugars in the endosperm of oilseed rape contribute to the flux of oil synthesis. While the hexose content of the liquid endosperm decreased during development the sucrose content increased. It is important to understand the relative rates of use of the endosperm sugars for two reasons. Firstly we need to know which sugars are used, and at what stages in development, in order to understand the roles of enzymes involved in their metabolism. Secondly, changes in sugar concentration have been implicated in the regulation of expression of genes determining storage-product synthesis [see Weber, Borisjuk and Wobus (1997) Trends Plant Sci. 2, 169–174, for review]. The rate of consumption of sugar is one factor governing its concentration. We present data showing both the concentration-dependence of conversion of sugar to oil, and the in vivo concentrations of sugars; we relate these data sets to each other and discuss the effects of the intracellular pool of sucrose. Glucose, fructose and sucrose are all substrates for oil synthesis, but the rates of their use (particularly sucrose) are underestimated because of dilution by sucrose from the intracellular pool.
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38

Khan, Riaz, and Gita Patel. "Branched-chain sucroses: Synthesis and Wittig reaction of the 1′-aldehydo derivative of sucrose." Carbohydrate Research 162, no. 2 (May 1987): 209–15. http://dx.doi.org/10.1016/0008-6215(87)80216-1.

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39

Yamashita, Yoshihisa, Kiyotaka Tomihisa, Yoshio Nakano, Yoshihiro Shimazaki, Takahiko Oho, and Toshihiko Koga. "Recombination between gtfB andgtfC Is Required for Survival of a dTDP-Rhamnose Synthesis-Deficient Mutant of Streptococcus mutans in the Presence of Sucrose." Infection and Immunity 67, no. 7 (July 1, 1999): 3693–97. http://dx.doi.org/10.1128/iai.67.7.3693-3697.1999.

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ABSTRACT The rml genes are involved in dTDP-rhamnose synthesis in Streptococcus mutans. A gene fusion betweengtfB and gtfC, which both encode extracellular water-insoluble glucan-synthesizing enzymes, accompanied by inactivation of the rml genes was observed for cells grown in the presence of sucrose. The survival rates of rmlmutants isolated in the absence of sucrose were drastically reduced in the presence of sucrose. The rates were consistent with the frequency of spontaneous gene fusions between gtfB andgtfC, suggesting that the spontaneous recombinant organisms were selected in the presence of sucrose. The rml mutants with a gtfB-gtfC fusion gene had markedly reduced water-insoluble glucan synthetic activity and lost the ability to colonize glass surfaces in the presence of sucrose. These results suggest that the rml mutants of S. mutans, which are defective in dTDP-rhamnose synthesis, can survive only in the absence of water-insoluble glucan synthesis.
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40

Dai, Yanjiao, Binglin Chen, Yali Meng, Wenqing Zhao, Zhiguo Zhou, Derrick M. Oosterhuis, and Youhua Wang. "Effects of elevated temperature on sucrose metabolism and cellulose synthesis in cotton fibre during secondary cell wall development." Functional Plant Biology 42, no. 9 (2015): 909. http://dx.doi.org/10.1071/fp14361.

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Global warming has the potential to increase air temperatures by 1.8 to 4.0°C by the end of the 21st century. In order to reveal the effects of increased temperatures on the sucrose metabolism and cellulose synthesis in cotton fibre during its flowering and boll formation stage, field experiments with elevated temperature regimes (32.6/28.6°C, mean daytime/night-time temperature during flowering and boll formation stage during 2010–12, the same below) and ambient temperature regimes (30.1/25.8°C) were conducted. Activities of sucrose synthase and acid/alkaline invertase decreased under elevated temperature in fibre, but activities of sucrose phosphate synthase were increased. Callose content increased, but sucrose content decreased within the cotton fibre under elevated temperature. The disparity of callose content and sucrose content between the two temperature regimes decreased with the number of days post anthesis, indicating that the effects of elevated temperature on both sucrose content and cellulose content were diminished as the boll matured. Due to the dynamics of the carbohydrate content and associated enzyme activities, we hypothesise that the restrained sucrose metabolism and cellulose biosynthesis under elevated temperatures were mainly attributed to the changed activities of sucrose synthase and invertase. Furthermore, 32.6/28.6°C had a negative effect on the cellulose synthesis compared with 30.1/25.8°C.
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41

Trevanion, Stephen J., C. Kate Castleden, Christine H. Foyer, Robert T. Furbank, W. Paul Quick, and John E. Lunn. "Regulation of sucrose-phosphate synthase in wheat (Triticum aestivum) leaves." Functional Plant Biology 31, no. 7 (2004): 685. http://dx.doi.org/10.1071/fp04038.

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The regulation of sucrose-phosphate synthase (SPS, E.C. 2.4.1.14), a key enzyme of sucrose synthesis, was investigated in wheat (Triticum aestivum L.) leaves. Wheat SPS was activated in the light, with an increased affinity for its substrates and the activator glucose-6-phosphate, reduced sensitivity to inhibition by Pi, but no change in maximum catalytic activity. Based on these properties, assays to measure the total activity and activation state of the enzyme were established and validated using several different wheat cultivars, grown under different environmental conditions. As found in previous studies on other species, e.g. spinach, activation appeared to be linked to the prevailing rate of photosynthesis rather than light per se. Long-term exposure to higher light levels increased total SPS activity in the leaves, and some experiments indicated that this response could occur within 1 h of exposure of low-light-grown plants to high light. However, activation of pre-existing enzyme was a more common short-term response to high light. Wheat, like many important cereal species, stores a large amount of sucrose in its leaves. In contrast with spinach, which stores more starch in its leaves, accumulation of sucrose in wheat leaves did not lead to inactivation of SPS or inhibition of sucrose synthesis. In conclusion, the mechanisms linking the rates of sucrose synthesis and photosynthetic CO2 fixation in wheat leaves appear to be similar to those in other species, but the mechanisms involved in short-term feedback inhibition of sucrose synthesis by sucrose, found in starch-storing species, are lacking in wheat.
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42

Jarosz, Sławomir, Patrycja Sokołowska, and Łukasz Szyszka. "Synthesis of fine chemicals with high added value from sucrose: Towards sucrose-based macrocycles." Tetrahedron Letters 61, no. 22 (May 2020): 151888. http://dx.doi.org/10.1016/j.tetlet.2020.151888.

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43

Römer, Ulrike, Nadja Nettelstroth, Walter Köckenberger, and Lothar Elling. "Characterization of Recombinant Sucrose Synthase 1 from Potato for the Synthesis of Sucrose Analogues." Advanced Synthesis & Catalysis 343, no. 6-7 (August 2001): 655–61. http://dx.doi.org/10.1002/1615-4169(200108)343:6/7<655::aid-adsc655>3.0.co;2-a.

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44

AHMADI, A., and D. A. BAKER. "The effect of water stress on grain filling processes in wheat." Journal of Agricultural Science 136, no. 3 (May 2001): 257–69. http://dx.doi.org/10.1017/s0021859601008772.

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The effect of water stress, commencing from the late cell division period, on in vivo grain growth was studied in relation to grain sucrose, water status and in vitro starch synthesis. Detached ear experiments were conducted to assess the effect of sink dehydration on grain filling processes under non-limiting source conditions. Water stress caused premature grain desiccation and resulted in a marked decline in grain sucrose and reduced grain weight. Both sucrose uptake and conversion to starch in vitro were increased by mild water stress (solute potential (Ψs)−0·8 MPa). However, a decline in Ψs below this optimum resulted in reduced sucrose uptake and starch synthesis not attributable to a reduced supply of sucrose. Stressed grains which failed to accumulate dry matter in vivo showed significant starch synthesis when cultured in vitro. Grains from in situ and osmotically stressed plants showed a lower capacity for starch synthesis in vitro. The results indicate that grain filling processes under stress conditions are limited by (1) low substrate availability and low Ψs within the sink i.e. an unfavourable seed environment (non-lasting effect) and (2) reduced synthetic capacity of the sink (carry-over effect).
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45

Grimaud, F., P. Faucard, L. Tarquis, S. Pizzut-Serin, P. Roblin, S. Morel, S. Le Gall, et al. "Enzymatic synthesis of polysaccharide-based copolymers." Green Chemistry 20, no. 17 (2018): 4012–22. http://dx.doi.org/10.1039/c8gc01251b.

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46

Suzuki, Akio, Yoshinori Kanayama, and Shohei Yamaki. "Occurrence of Two Sucrose Synthase Isozymes during Maturation of Japanese Pear Fruit." Journal of the American Society for Horticultural Science 121, no. 5 (September 1996): 943–47. http://dx.doi.org/10.21273/jashs.121.5.943.

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The properties of sucrose synthase (SS) isozymes partially purified from immature fruit (SS I) of Japanese pear (Pyrus serotina Rehder var. culta Rehder) were different than those of mature fruit (SS II). A clear difference in elusion pattern during DEAE-cellulose chromatography was observed, although the apparent molecular weight of the native proteins extracted from both stages was 350 kD. The Km value of SS II for UDP was similar to that for UDP-glucose; while with SS I, the Km for UDP was lower than that for UDP-glucose. This suggests that SS II activity favors sucrose synthesis compared with SS I, which favors sucrose cleavage. The optimum pH for activity toward sucrose synthesis was 8.0 for SS II and 8.5 to 9.5 for SS I. SS II from mature fruit may be an isozyme of SS occurring during periods of rapid sucrose accumulation, while SS I from immature fruit is more similar to the typical SS which functions mainly toward sucrose cleavage in many plants.
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47

Li, Cong, Yu Liu, Jing Tian, Yanshu Zhu, and Jinjuan Fan. "Changes in sucrose metabolism in maize varieties with different cadmium sensitivities under cadmium stress." PLOS ONE 15, no. 12 (December 11, 2020): e0243835. http://dx.doi.org/10.1371/journal.pone.0243835.

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Sucrose metabolism contributes to the growth and development of plants and helps plants cope with abiotic stresses, including stress from Cd. Many of these processes are not well-defined, including the mechanism underlying the response of sucrose metabolism to Cd stress. In this study, we investigated how sucrose metabolism in maize varieties with low (FY9) and high (SY33) sensitivities to Cd changed in response to different levels of Cd (0 (control), 5, 10, and 20 mg L−1 Cd). The results showed that photosynthesis was impaired, and the biomass decreased, in both varieties of maize at different Cd concentrations. Cd inhibited the activities of sucrose phosphate synthase (SPS) and sucrose synthase (SS) (sucrose synthesis), and stimulated the activities of acid invertase (AI) and SS (sucrose hydrolysis). The total soluble sugar contents were higher in the Cd-treated seedlings than in the control. Also, Cd concentrations in the shoots were higher in SY33 than in FY9, and in the roots were lower in SY33 than in FY9. The decreases in the photosynthetic rate, synthesis of photosynthetic products, enzyme activity in sucrose synthesis direction, and increases in activity in hydrolysis direction were more obvious in SY33 (the sensitive variety) than in FY9 (the tolerant variety), and more photosynthetic products were converted into soluble sugar in SY33 than in FY9 as the Cd stress increased. The transcript levels of the sugar transporter genes also differed between the two varieties at different concentrations of Cd. These results suggest that sucrose metabolism may be a secondary response to Cd additions, and that the Cd-sensitive variety used more carbohydrates to defend against Cd stress rather than to support growth than the Cd-tolerant variety.
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48

Gajda, Norbert, and Sławomir Jarosz. "Synthesis of macrocyclic derivatives with di-sucrose scaffold." Arkivoc 2017, no. 2 (July 13, 2016): 76–86. http://dx.doi.org/10.3998/ark.5550190.p009.719.

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49

Chan, Anita Wai-Yin, and Bruce Ganem. "A Regioselective, Chemoenzymatic Synthesis of Sucrose- 1′-Methacrylate." Biocatalysis 8, no. 2 (January 1993): 163–69. http://dx.doi.org/10.3109/10242429308998203.

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50

Gagnaire, Juliette, Gülsen Toraman, Gérard Descotes, Alain Bouchu, and Yves Queneau. "Synthesis in water of amphiphilic sucrose hydroxyalkyl ethers." Tetrahedron Letters 40, no. 14 (April 1999): 2757–60. http://dx.doi.org/10.1016/s0040-4039(99)00321-4.

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