Academic literature on the topic 'Sucrose Synthesis'
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Journal articles on the topic "Sucrose Synthesis"
Bhuvaneswari, E., B. Sailaja, and S. Sivaprasad. "Impact of photoperiod on circadian sucrose and sucrase rhythms in the digestive system of silkworm, Bombyx mori." Journal of Applied and Natural Science 5, no. 1 (June 1, 2013): 230–41. http://dx.doi.org/10.31018/jans.v5i1.312.
Full textDyer, Ulrich C., and Yoshito Kishi. "Synthesis of C-sucrose." Journal of Organic Chemistry 53, no. 14 (July 1988): 3383–84. http://dx.doi.org/10.1021/jo00249a056.
Full textLunn, John Edward. "Evolution of Sucrose Synthesis." Plant Physiology 128, no. 4 (April 1, 2002): 1490–500. http://dx.doi.org/10.1104/pp.010898.
Full textSuzuki, Michio, and Christopher J. Pollock. "Extraction and characterization of the enzymes of fructan biosynthesis in timothy (Phleum pratense)." Canadian Journal of Botany 64, no. 9 (September 1, 1986): 1884–87. http://dx.doi.org/10.1139/b86-250.
Full textNakai, Tomonori, Naoto Tonouchi, Takayasu Tsuchida, Hitoshi Mori, Fukumi Sakai, and Takahisa Hayashi. "Synthesis of Asymmetrically Labeled Sucrose by a Recombinant Sucrose Synthase." Bioscience, Biotechnology, and Biochemistry 61, no. 11 (January 1997): 1955–56. http://dx.doi.org/10.1271/bbb.61.1955.
Full textLay, Luigi, Francesco Nicotra, Cristina Pangrazio, Luigi Panza, and Giovanni Russo. "Synthesis of antimetabolites of sucrose." Journal of the Chemical Society, Perkin Transactions 1, no. 3 (1994): 333. http://dx.doi.org/10.1039/p19940000333.
Full textSong, Zi-juan, Shu-jun Li, Xi Chen, Li-mei Liu, and Zhan-qian Song. "Synthesis of insecticidal sucrose esters." Forestry Studies in China 8, no. 3 (September 2006): 26–29. http://dx.doi.org/10.1007/s11632-006-0019-2.
Full textHisajima, S., Y. Arai, and T. A. Thorpe. "Sucrose synthesis in callus cultures." Biologia Plantarum 27, no. 1 (January 1985): 74–77. http://dx.doi.org/10.1007/bf02894639.
Full textEhira, Shigeki, Satoshi Kimura, Shogo Miyazaki, and Masayuki Ohmori. "Sucrose Synthesis in the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain PCC 7120 Is Controlled by the Two-Component Response Regulator OrrA." Applied and Environmental Microbiology 80, no. 18 (July 7, 2014): 5672–79. http://dx.doi.org/10.1128/aem.01501-14.
Full textSzyszka, Łukasz, Piotr Cmoch, Aleksandra Butkiewicz, Mykhaylo A. Potopnyk, and Sławomir Jarosz. "Synthesis of Cyclotriveratrylene-Sucrose-Based Capsules." Organic Letters 21, no. 16 (August 7, 2019): 6523–28. http://dx.doi.org/10.1021/acs.orglett.9b02451.
Full textDissertations / Theses on the topic "Sucrose Synthesis"
Baik, Youngmin. "Carbothermal synthesis of aluminum nitride using sucrose." Thesis, McGill University, 1991. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=60643.
Full textHawker, John Seth. "Sucrose and starch metabolism in leaves, storage organs and developing fruits of higher plants." Title page, contents and summary only, 1988. http://web4.library.adelaide.edu.au/theses/09SD/09sdh392.pdf.
Full textLunn, John Edward. "The control of sucrose synthesis in non-photosynthetic tissues." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304460.
Full textKochhar, Anuradha. "Assimilate partitioning and sucrose synthesis in Lamium album L." Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624185.
Full textVerges, Alizee. "Computer-aided design and engineering of sucrose-utilizing transglucosylases for oligosaccharide synthesis." Thesis, Toulouse, INSA, 2015. http://www.theses.fr/2015ISAT0020/document.
Full textChemical synthesis of complex oligosaccharides still remains critical. Enzymes have emerged as powerful tools to circumvent chemical boundaries of glycochemistry. However, natural enzymes do not necessarily display the required properties and need to be optimized by molecular engineering. Combined use of chemistry and tailored biocatalysts may thus be attractive for exploring novel synthetic routes, especially for glyco-based vaccines development. The objective of this thesis was thus to apply semi-rational engineering strategies to Neisseria polysaccharea amylosucrase (NpAS), a sucrose-utilizing α-transglucosylase, in order to conceive novel substrate specificities and extend the potential of this enzyme to catalyze novel reactions, going beyond what nature has to offer. In a first study, a computer aided-approach was followed to reshape the active site of the enzyme (subsites +1, +2 and +3) for the recognition and α-1,4 glucosylation of a non-natural disaccharide acceptor molecule (allyl 2-deoxy-2-N-trichloroacetyl-β-D-glucopyranosyl-(1→2)-α-L-rhamnopyranose). The trisaccharide product is a building block for the chemo-enzymatic synthesis of oligosaccharides mimicking the repetitive units of the Shigella flexneri lipopolysaccharides, and ultimately, for the production of a vaccine against Shigellosis disease. Using computational tools dedicated to the automated protein design, combined with sequence analysis, a library of about 2.7x104 sequences was designed and experimentally constructed and screened. Altogether, 55 mutants were identified to be active on sucrose (the donor substrate), and one, called mutant F3, was subsequently found able to catalyze the α-1,4 glucosylation of the target disaccharide. Impressively, this mutant contained seven mutations in the first shell of the active site leading to a drastic reshaping of the catalytic pocket without significantly perturbing the original specificity for sucrose donor substrate. In a second study, three variants were identified from the screening of the semi-rational library on sole sucrose as displaying totally novel product specificities. They were further characterized, as well as their products, at both biochemical and structural level. These mutants, called 37G4, 39A8 and 47A10, contained between 7 and 11 mutations into their active site. They were found able to use sucrose and maltose (a reaction product from sucrose) as both donor and acceptor substrates to produce in varying amounts erlose (α-D-Glucopyranosyl-(1→4)-α-D-Glucopyranosyl-(1→2)-β-D-Fructose) and panose (α-D-Glucopyranosyl-(1→6)-α-D-Glucopyranosyl-(1→4)-α-D-glucose) trisaccharides, which are not produced at all by parental wild-type enzyme. Relatively high yields were obtained for the production of these molecules, which are known to have acariogenic and sweetening properties and could be of interest for food applications. In a last part, another mutant 30H3 was isolated due to its high activity on sucrose (6.5-fold improvement compared to wild-type activity) from primary screening of the library. When characterized, the mutant revealed a singular product profile compared to that of wild-type NpAS. It appeared highly efficient for the synthesis of soluble maltooligosaccharides of controlled size chains, from DP 3 to 21, and with a low polydispersity. No formation of insoluble polymer was found. The X-ray structure of the mutant was determined and revealed the opening of the catalytic pocket due to the presence of 9 mutations in the first sphere. Molecular dynamics simulations suggested a role of mutations onto flexibility of domain B’ that might interfere with oligosaccharide binding and explain product specificity of the mutant
Baguma, Yona. "Regulation of starch synthesis in cassava /." Uppsala : Dept. of Plant Biology and Forest Genetics, Swedish Univ. of Agricultural Sciences, 2004. http://epsilon.slu.se/a478.pdf.
Full textKonishi, Teruko. "THE EXPRESSION OF SUCROSE SYNTHASE AND ITS ROLE IN PLANT β-GLUCAN SYNTHESIS." Kyoto University, 2002. http://hdl.handle.net/2433/149898.
Full text0048
新制・課程博士
博士(農学)
甲第9606号
農博第1234号
新制||農||841(附属図書館)
学位論文||H14||N3638(農学部図書室)
UT51-2002-G364
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 酒井 富久美, 教授 關谷 次郎, 教授 島田 幹夫
学位規則第4条第1項該当
Gerber, Jacqués. "The phloem unloading and sucrose-sequestration pathway in the internodal stem tissue of the Saccharum hybrid var. NCo376." Thesis, Rhodes University, 2001. http://hdl.handle.net/10962/d1003763.
Full textNell, Hanlie. "Genetic manipulation of sucrose-storing tissue to produce alternative products." Thesis, Link to the online version, 2007. http://hdl.handle.net/10019/1136.
Full textTrollope, Kim Mary. "Engineering a fungal β-fructofuranosidase." Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/96757.
Full textENGLISH ABSTRACT: β-fructofuranosidases are hydrolytic enzymes that act on sucrose to yield the products glucose and fructose. Under high substrate conditions these enzymes display fructosyltransferase activity which results in the synthesis of fructooligosaccharides (FOS). Some enzymes display higher propensities for FOS synthesis than others, with the determinants of this activity remaining unclear. The consumption of FOS produces a prebiotic effect that positively alters the composition of the colonic microflora, and as a result is linked to improved human and animal health. The increased demand for FOS has necessitated the industrial production of these nutraceuticals. In enzymatic sucrose biotransformation processes operating at high substrate loading and temperatures between 50 and 60°C, β-fructofuranosidase activity is negatively influenced by glucose product inhibition and thermal instability. The aim of this study was therefore to engineer the Aspergillus japonicus β-fructofuranosidase, FopA, to improve a FOS synthesis bioprocess. A dual approach was employed to engineer FopA so as to increase the probability of obtaining an improved enzyme variant(s). A random mutagenesis approach was applied to harness the potential of the randomness of introduced mutations as precise structural knowledge of the enzyme regions involved in the phenotypic presentation of product inhibition, specific activity and thermal stability was unavailable. A semi-rational approach afforded the additional opportunity to reduce the number of variants to be screened, yet theoretically increased the functional content of the library. This study details the development of a method to rapidly quantify FOS using Fourier transform mid infrared attenuated total reflectance spectroscopy and multivariate data analysis. The method offers improvements over conventionally used high performance liquid chromatography in terms of reduced sample analysis times and the absence of toxic waste products. This is the first report on the direct screening of an enzyme variant library for FOS synthesis to identify improved variants and will significantly support future engineering of β-fructofuranosidases using random mutagenesis approaches. The random mutagenesis approach yielded a variant displaying limited relief from glucose inhibition. At the peak difference in performance, the variant produced 28% more FOS from the same amount of sucrose, when compared to the parent. The semi-rational approach, using a combined crystal structure and evolutionary-guided approach, yielded a four amino acid combination variant displaying improved specific activity and thermostability that was able to reduce the time to completion of an industrial-like FOS synthesis reaction by 26%. The positive outcome of the semi-rational approach showed that engineering loops regions in an enzyme is a feasible strategy to improve both specific activity and thermostability, most probably due to the modification of enzyme structural flexibility. A bioinformatic tool that enables the identification of β-fructofuranosidases displaying high-level FOS synthesis from protein sequence alone was also developed during the study. These investigations revealed conserved sequence motifs characteristic of enzymes displaying low- and high-level FOS synthesis and a structural loop, unique to the latter group, that were readily applicable identifiers of FOS synthesis capacity. The tool presented may also be useful to improve the understanding of the structure-function relationships of β-fructofuranosidases by facilitating the identification of variations in groups of enzymes that have been functionally sub-classified.
AFRIKAANSE OPSOMMING: β-fruktofuranosidases is hidrolitiese ensieme wat op sukrose inwerk en glukose en fruktose as produkte vorm. Onder toestande met hoë substraatkondisies vertoon hierdie ensieme fruktosieltransferase-aktiwiteit wat tot die sintese van frukto-oligosakkariede (FOS) lei. Sommige ensieme neig na ʼn hoër FOS-sintese as ander, maar die bepalende faktore vir hierdie aktiwiteit is nog onbekend. Die verbruik van FOS veroorsaak ʼn prebiotiese effek wat die samestelling van kolon mikroflora positief beïnvloed en met verhoogde mens- en dieregesondheid verbind word. Die verhoogde aanvraag vir FOS het die industriële produksie van hierdie nutraseutiese middel genoodsaak. Tydens ensiemgedrewe sukrose-biotransformasieprosesse by hoë substraatladings en temperature tussen 50 en 60 °C, word β-fruktofuranosidase-aktiwiteit negatief deur glukose produkonderdrukking en termiese onstabiliteit beïnvloed. Die doel van hierdie studie was dus om die Aspergillus japonicus β-fruktofuranosidase, FopA, vir ʼn verbeterde FOS-sintese bioproses te manipuleer. ʼn Tweeledige benadering is vir FopA manipulasie gevolg om die waarskynlikheid van verbeterde variant(e) te verhoog. ʼn Lukrake mutagenese benadering, wat die potensiaal van ingevoegde mutasie ewekansigheid inspan, is in die lig van onvoldoende akkurate kennis van die strukturele gedeeltes betrokke by produkinhibisie-, spesifieke aktiwiteit- en termiese stabiliteit fenotipes gevolg. Die toepassing van ʼn semi-rasionele benadering het ook geleentheid vir die sifting van ʼn kleiner variantbibioloteek geskep, terwyl die funksionele inhoud teoreties verhoog word. Die studie beskryf die ontwikkeling van ʼn metode vir die vinnige kwantifisering van FOS, gebaseer op Fourier transform middel infrarooi geattenueerde totale refleksie spektroskopie en meerveranderlike data-analise. Dit is die eerste melding van ʼn direkte sifting van ʼn ensiemvariantversameling vir FOS-sintese om verbeterde variante te identifiseer, en kan die toekomstige manipulasie van β-fruktofuranosidases deur middel van lukrake mutagenese-benaderings beduidend ondersteun. Die lukrake mutagenese-benadering het ʼn variant met beperkte opheffing van glukose-onderdrukking gelewer. By die punt waar die prestasie die meeste verskil, het die variant 28% meer FOS vanaf dieselfde hoeveelheid sukrose geproduseer in vergelyking met die ouer-ensiem. Die semi-rasionele benadering, gegrond op ʼn kombinasie van kristalstruktuur en evolusionêre-geleide benaderings, het ʼn vier-aminosuurkombinasie variant met hoër spesifieke aktiwiteit en termostabiliteit gelewer wat die voltooiingstyd van ʼn tipiese industriële FOS sintesereaksie met 26% kon verkort. Die positiewe uitkoms van die semi-rasionele benadering het aangedui dat manipulasie van die lusgedeeltes in ʼn ensiem ʼn lewensvatbare strategie is om beide spesifieke aktiwiteit en termostabiliteit te verbeter, moontlik as gevolg van wysigings in die buigsaamheid van die ensiemstruktuur. ʼn Bioïnformatika-hulpmiddel vir die identifikasie van β-fruktofuranosidases met hoë vlakke van FOS-sintese op grond van proteïenvolgordes is ook tydens die studie ontwikkel. Motiewe met gekonserveerde volgordes kenmerkend van lae- en hoë-vlak FOS-produserende ensieme en ʼn strukturele lus, uniek tot die laasgenoemde groep, is tydens die ondersoek onthul wat as maklike identifiseerders van FOS-sintesekapasiteit kan dien. Die voorgestelde hulpmiddel kan ook nuttig wees om die struktuur-funksie-verwantskap van β-fruktofuranosidases beter te verstaan deur die identifikasie van variasie in ensiemgroepe wat funksioneel gesubklassifiseer is.
Books on the topic "Sucrose Synthesis"
John, Philip. Biosynthesis of the major crop products: The biochemistry, cell physiology, and molecular biology involved in the synthesis by crop plants of sucrose, fructan, starch, cellulose, oil, rubber, and protein. Chichester: Wiley, 1992.
Find full textKonowicz, Paul A. Some synthetic transformations on sucrose. Norwich: University of East Anglia, 1991.
Find full textBook chapters on the topic "Sucrose Synthesis"
Stitt, Mark. "Limitation of Photosynthesis by Sucrose Synthesis." In Progress in Photosynthesis Research, 685–92. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0516-5_144.
Full textSharkey, Thomas D., Marianne M. Laporte, Barry J. Micallef, Christine K. Shewmaker, and Jannette V. Oakes. "Sucrose Synthesis, Temperature, and Plant Yield." In Photosynthesis: from Light to Biosphere, 4527–32. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-009-0173-5_1063.
Full textHeldt, Hans W., and Mark Stitt. "The Regulation of Sucrose Synthesis in Leaves." In Progress in Photosynthesis Research, 675–84. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0516-5_143.
Full textJo, Seongbong, and Kinam Park. "Synthesis and Characterization of Thermoreversible Sucrose Hydrogels (Sucrogels)." In ACS Symposium Series, 113–26. Washington, DC: American Chemical Society, 1999. http://dx.doi.org/10.1021/bk-1999-0737.ch008.
Full textStitt, Mark. "Application of Control Analysis to Photosynthetic Sucrose Synthesis." In Control of Metabolic Processes, 363–76. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4757-9856-2_32.
Full textHAYASHI, TAKAHISA, TERUKO KONISHI, YASUNORI OHMIYA, and TOMONORI NAKAI. "Is cellulose synthesis enhanced by expression of sucrose sysnthesis in poplar." In Abiotic stress tolerance in plants, 187–93. Dordrecht: Springer Netherlands, 2006. http://dx.doi.org/10.1007/1-4020-4389-9_13.
Full textZervosen, Astrid, and Lothar Elling. "Application of Sucrose Synthase in the Synthesis of Nucleotide Sugars and Saccharides." In Carbohydrate Biotechnology Protocols, 235–54. Totowa, NJ: Humana Press, 1999. http://dx.doi.org/10.1007/978-1-59259-261-6_19.
Full textPaulino, C., and M. C. Arrabaça. "Synthesis of Sucrose and Fructans in Wheat Leaves: The Effects of Temperature." In Current Research in Photosynthesis, 3453–56. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-0511-5_777.
Full textSuyama, Kyozo, and Susumu Adachi. "Enzymatic synthesis of new trisaccharide, isoraffinose, from a mixture of lactose and sucrose." In MILK the vital force, 223. Dordrecht: Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-3733-8_182.
Full textYamada, Kouichi, and Jun Takezawa. "Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation." In Subcellular Biochemistry, 435–38. Dordrecht: Springer Netherlands, 2006. http://dx.doi.org/10.1007/978-1-4020-4896-8_42.
Full textConference papers on the topic "Sucrose Synthesis"
Elling, Lothar, Ulrike Römer, and Walter Knöckenberger. "Characterization and Application of Recombinant Sucrose Synthase 1 from Potato for the Synthesis of Sucrose Analogues." In The 4th International Electronic Conference on Synthetic Organic Chemistry. Basel, Switzerland: MDPI, 2000. http://dx.doi.org/10.3390/ecsoc-4-01930.
Full textJin, Li, Fangtong Liu, and Jianpo Zhang. "THE SYNTHESIS AND CHARACTERIZATION OF SUCROSE MODIFIED MAGNETITE." In International Conference on New Materials and Intelligent Manufacturing (ICNMIM). Volkson Press, 2018. http://dx.doi.org/10.26480/icnmim.01.2018.164.166.
Full textLi, Quanhui, Jiaying Xin, Tingting Yao, Zhengyang Wang, and Luoyun Zheng. "The Microwave-assisted Lipase-catalyzed Synthesis of Sucrose Laurate." In International Conference on Electronics, Mechanics, Culture and Medicine. Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/emcm-15.2016.64.
Full textMaria, M. Ibadurrohman, and Slamet. "Synthesis of sucrose ester surfactant by utilizing molasses and waste cooking oil." In INTERNATIONAL CONFERENCE ON TRENDS IN MATERIAL SCIENCE AND INVENTIVE MATERIALS: ICTMIM 2020. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0013809.
Full textNaulidia, R. A., E. Juliana, S. Handayani, F. Damayanti, S. Setiasih, and S. Hudiyono. "Enzymatic synthesis of glycerol and sucrose-palm oil fatty acid esters produced and their potency as antimicrobial agents." In THE 8TH INTERNATIONAL CONFERENCE OF THE INDONESIAN CHEMICAL SOCIETY (ICICS) 2019. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0001346.
Full textTitinchi, Salam J. J., Waheed Saban, Leslie Petrik, and Hanna S. Abbo. "Synthesis, Characterization and Physiochemical Properties of Platinum Supported on Mesoporous Carbon." In ASME 2011 9th International Conference on Fuel Cell Science, Engineering and Technology collocated with ASME 2011 5th International Conference on Energy Sustainability. ASMEDC, 2011. http://dx.doi.org/10.1115/fuelcell2011-54670.
Full textBezler, N. V., and M. Yu Petyurenko. "Bacteria of Pseudomonas genus in sugar beet agrocenosis." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.043.
Full textCuppoletti, John. "Composite Synthetic Membranes Containing Native and Engineered Transport Proteins." In ASME 2008 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. ASMEDC, 2008. http://dx.doi.org/10.1115/smasis2008-449.
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