Journal articles on the topic 'Sucrose Storage'
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Madritsch, Silvia, Svenja Bomers, Alexandra Posekany, Agnes Burg, Rebekka Birke, Florian Emerstorfer, Reinhard Turetschek, Sandra Otte, Herbert Eigner, and Eva M. Sehr. "Integrative transcriptomics reveals genotypic impact on sugar beet storability." Plant Molecular Biology 104, no. 4-5 (August 4, 2020): 359–78. http://dx.doi.org/10.1007/s11103-020-01041-8.
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Abstract Key message An integrative comparative transcriptomic approach on six sugar beet varieties showing different amount of sucrose loss during storage revealed genotype-specific main driver genes and pathways characterizing storability. Abstract Sugar beet is next to sugar cane one of the most important sugar crops accounting for about 15% of the sucrose produced worldwide. Since its processing is increasingly centralized, storage of beet roots over an extended time has become necessary. Sucrose loss during storage is a major concern for the sugar industry because the accumulation of invert sugar and byproducts severely affect sucrose manufacturing. This loss is mainly due to ongoing respiration, but changes in cell wall composition and pathogen infestation also contribute. While some varieties can cope better during storage, the underlying molecular mechanisms are currently undiscovered. We applied integrative transcriptomics on six varieties exhibiting different levels of sucrose loss during storage. Already prior to storage, well storable varieties were characterized by a higher number of parenchyma cells, a smaller cell area, and a thinner periderm. Supporting these findings, transcriptomics identified changes in genes involved in cell wall modifications. After 13 weeks of storage, over 900 differentially expressed genes were detected between well and badly storable varieties, mainly in the category of defense response but also in carbohydrate metabolism and the phenylpropanoid pathway. These findings were confirmed by gene co-expression network analysis where hub genes were identified as main drivers of invert sugar accumulation and sucrose loss. Our data provide insight into transcriptional changes in sugar beet roots during storage resulting in the characterization of key pathways and hub genes that might be further used as markers to improve pathogen resistance and storage properties.
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Rosche, Elke G., Daniel Blackmore, Christina E. Offler, and John W. Patrick. "Increased capacity for sucrose uptake leads to earlier onset of protein accumulation in developing pea seeds." Functional Plant Biology 32, no. 11 (2005): 997. http://dx.doi.org/10.1071/fp05127.
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Pea (Pisum sativum L.) cotyledons, overexpressing a potato sucrose transporter (StSUT1), were used to explore the hypothesis that sucrose stimulates the onset of storage protein biosynthesis. The study focused on the transition between pre-storage and storage phases of seed development. During this period supply of sucrose and hexose to transgenic cotyledons was unaffected by StSUT1 expression. However, protoplasmic levels of sucrose but not hexoses were elevated in transgenic cotyledons. Total protein levels in cotyledons followed the same temporal trend as observed for sucrose and this was reflected in an earlier appearance of protein bodies. Protein levels in wild type and StSUT1 cotyledons were found to lie on the same sucrose dose-response curve and this could be reproduced in vitro when wild type cotyledons were cultured on media containing various sucrose concentrations. Rates of [14C]sucrose uptake and incorporation into polymeric forms were consistent with protoplasmic sucrose supplying a proportion of the carbon skeletons required for storage protein accumulation. In addition, vicilin gene expression was up-regulated earlier in StSUT1 cotyledons. We conclude that sucrose functions both as a signal and fuel to stimulate storage protein accumulation and assembly into protein bodies. An earlier stimulation of storage protein synthesis is considered to largely account for the 14% increase in protein levels of StSUT1 seeds at harvest.
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Gąsecka, Monika, Jerzy Stachowiak, Włodzimierz Krzesiński, Mikołaj Knaflewski, and Piotr Goliński. "Changes in Glucose, Fructose and Sucrose Contents in Storage Roots of Asparagus During Vegetation Period." Vegetable Crops Research Bulletin 69, no. 1 (January 1, 2008): 145–54. http://dx.doi.org/10.2478/v10032-008-0029-9.
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Changes in Glucose, Fructose and Sucrose Contents in Storage Roots of Asparagus During Vegetation PeriodThe objective of the field experiment conducted during 2000-2002 was to determine changes in glucose, fructose and sucrose contents in storage roots of asparagus (Asparagus officinalisL.) cv.‘Thielim’during vegetation period. The aim of the study was also to estimate the correlation between yield and the content of carbohydrates. Sum of glucose, fructose and sucrose contents (GFS) and sucrose contents in storage roots of asparagus decreased at the beginning and increased at the end of harvest. Generally glucose and fructose for carbohydrate contents increased, while that of sucrose decreased. A possitive correlation was observed for sucrose and GFS between asparagus spears and storage roots (r=0.821 and r=0.641, respectively). A negative correlation between the yield of spears and glucose, sucrose and GFS contents in storage roots was found (r=0.595, r=0.624, r=0.794, respectively). Positive correlations were found between total yield during harvest and year of cropping, average GFS content in storage roots during harvest, sum of radiation during harvest, while negative correlation between total yield and sum of average daily air temperature during harvest was found.
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Lee, Pui-Yuei, Yien-Ping Wong, Shuhaidah Othman, and Hui-Yee Chee. "Room-temperature stable loop-mediated isothermal amplification (LAMP) reagents to detect leptospiral DNA." Asian Biomedicine 15, no. 4 (August 1, 2021): 183–89. http://dx.doi.org/10.2478/abm-2021-0023.
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Abstract Background Loop-mediated isothermal amplification (LAMP) is one of the most promising tools for rapidly detecting Leptospira spp. However, LAMP is hampered by cold storage to maintain the enzymatic activity of Bst DNA polymerase. Objective To overcome the drawback of cold storage requirement for LAMP reagents we modified the reagents by adding sucrose as stabilizer. We then sought to determine the stability at room temperature of the premixed LAMP reagents containing sucrose. Method Premixed LAMP reagents with sucrose and without sucrose were prepared. The prepared mixtures were stored at room temperature for up to 60 days, and were subjected to LAMP reactions at various intervals using rat kidney samples to detect leptospiral DNA. Results The premixed LAMP reagents with sucrose remained stable for 45 days while sucrose-free premixed LAMP reagents showed no amplification from day 1 of storage at room temperature up to day 14. Conclusion The LAMP reagent system can be refined by using sucrose as stabilizer, thus allowing their storage at room temperature without the need for cold storage. The modified method enables greater feasibility of LAMP for field surveillance and epidemiology in resource-limited settings.
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Campbell, L. G., and K. L. Klotz. "Characterizing sugarbeet varieties for postharvest storage losses is complicated by environmental effects and genotype × environment interactions." Canadian Journal of Plant Science 87, no. 1 (January 1, 2007): 121–27. http://dx.doi.org/10.4141/p06-085.
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Each year millions of tons of sugarbeet (Beta vulgaris L.) roots are stored in large exposed piles prior to processing. During postharvest storage, respiration and invert sugar formation consume sucrose and even a small reduction in these losses would have substantial economic impact. This study investigated the relative importance of hybrid, environment, and hybrid × environment interactions and examined their implications in characterizing hybrids for sucrose loss during storage or developing hybrids with improved storage properties. Glucose, fructose, and extractable sucrose concentrations and respiration rate were measured 30 and 120 d after harvest (DAH) on five hybrids produced in six environments. Environment effects were significant on both dates for all traits except fructose 30 DAH. Significant hybrid × environment interactions were observed for respiration rate 30 and 120 DAH, for extractable sucrose 120 DAH, and for glucose concentration 30 DAH. The only trait with a significant hybrid main effect was extractable sucrose 30 DAH. For the 90 d between measurements, extractable sucrose losses for individual hybrid-environment combinations ranged from 1 to 63% of the sucrose available 30 DAH. It appeared that large environmental impacts and hybrid × environment interactions, compared to the relatively small hybrid influences, would complicate selecting parental lines with all or most of the storage traits desired. Furthermore, a comprehensive evaluation of commercial hybrids or breeding lines for storage traits would require considerable resources. Efforts to understand the impact of production practices and growing season environment on storage properties would probably be more productive than attempting to produce commercial hybrids with improved storage characteristics. Key words: Beta vulgaris L., respiration, glucose, fructose, extractable sucrose
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Weber, Hans, Ute Heim, Sabine Golombek, Ljudmilla Borisjuk, and Ulrich Wobus. "Assimilate uptake and the regulation of seed development." Seed Science Research 8, no. 3 (September 1998): 331–46. http://dx.doi.org/10.1017/s0960258500004268.
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AbstractSeed development is a series of events involving cell division, followed by cell differentiation and storage activity In legume cotyledons, cell differentiation starts in certain regions and gradually spreads to other parts, thereby building up developmental gradients The entire process appears to be subject to metabolic control The high hexose state of the premature legume embryo as controlled by seed coat-specific invertases favours cell division Differentiation is initiated when hexose decreases and sucrose increases Seed development occurs in a close interaction with seed metabolism and transport processes Movement of photoassimilates from the sieve tubes to the unloading region of the maternal seed tissue is symplasmic and controlled by plasmodesmal passage Sucrose uptake into Vicia faba cotyledons is mediated by a H+-sucrose symporter located in the outer epidermis which generates transfer cells Formation of the sucrose uptake system is induced during the early to mid-cotyledon stage by tissue contact with the maternal seed coat and is controlled by carbohydrate availability In contrast, a hexose transporter gene is also expressed in epidermal cells covering younger, mitotically active regions of the cotyledons The sucrose uptake system apparently generates the high sucrose state immediately preceding the storage phase Sucrose specifically induces storage-associated differentiation processes indicating a specific sucrose-dependent signalling pathway operating in maturing cotyledons Moreover, the mode of sucrose uptake — apoplasmic movement into the epidermal cells with subsequent symplasmic transfer to the storage parenchyma cells — appears to control coordinated cotyledon development Unlike sucrose, amino acid transport into legume cotyledons is passive during early development but at later stages when large amounts of storage proteins are synthesized an additional active uptake system is established to ensure a sufficient supply
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Almeida, Elka Fabiana Aparecida, Patrícia Duarte de Oliveira Paiva, Luiz Carlos de Oliveira Lima, Franklin Cordeiro Silva, Juliana Fonseca, and Denismar Alves Nogueira. "Calla lily inflorescences postharvest: pulsing with different sucrose concentrations and storage conditions." Ciência e Agrotecnologia 35, no. 4 (August 2011): 657–63. http://dx.doi.org/10.1590/s1413-70542011000400003.
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Calla lily is an appreciated specie used for flower arrangements. In spite of its commercial importance, there is little information on calla lily postharvest conservation. Thus, this study aimed to determine the best sucrose concentration for pulsing and cold storage conditions to extend calla lily postharvest durability. Flower stalks were submitted to a pulsing pre-treatment using 2, 4, 8, 12 and 16% sucrose in the solution, for one hour, plus a treatment with direct storage in cold chamber (4ºC), without a prior-treatment. Dry storage or storage in solution with the commercial product Flower® was also tested. A completely randomized design was used with four replicates and three inflorescences per plot. Spathe length and width were daily measured from which the opening and wilting processes were analyzed. It was observed that pulsing with sucrose was efficient in extending calla lily inflorescences opening process and durability. Dry storage for short periods (less than six days) can also be used, but a prior-treatment with 12% sucrose pulsing for one hour or with a water supply for the same period was required. For storage in solution, a pulsing with 5% or 7.5% sucrose was recommended.
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La Bonte, Don R., David H. Picha, and Hester A. Johnson. "Carbohydrate-Related Changes in Sweetpotato Storage Roots during Development." Journal of the American Society for Horticultural Science 125, no. 2 (March 2000): 200–204. http://dx.doi.org/10.21273/jashs.125.2.200.
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The quantity and pattern of carbohydrate-related changes during storage root development differed among six sweetpotato cultivars [Ipomoea batatas (L.) Poir. `Beauregard', `Heart-o-Gold', `Jewel', `Rojo Blanco', `Travis', and `White Star']. Measurements were taken for individual sugars, total sugars, alcohol-insoluble solids (AIS, crude starch), and dry weight (DW) at 2-week intervals from 7 to 19 weeks after transplanting (WAT) in two separate years. Sucrose was the major sugar during all stages of development, representing at least 68% of total sugars across all cultivars and dates. Pairwise comparisons showed `Heart-o-Gold' had the highest sucrose content among the cultivars. Sucrose content increased by 56% for `Heart-o-Gold' over the 12 weeks of assay, ranking first among the cultivars at 17 and 19 WAT and possessing 27% more sucrose than the next highest ranking cultivar, `Jewel', at 19 WAT. Fructose content profiles varied among and within cultivars. `Beauregard' showed a consistent increase in fructose throughout development while `Whitestar' showed a consistent decrease. The other cultivars were inconsistent in their fructose content profiles. Glucose content profiles were similar to those for fructose changes during development. The relationship between monosaccharides was fructose = 0.7207 × glucose + 0.0241. Cultivars with the highest fructose and glucose content could be selected by breeders after 13 WAT. Early clonal selection for high sucrose and total sugars is less promising because substantive changes in clonal rank occurred for sucrose and total sugars after 15 WAT. Cultivars ranking the highest in total sugars had either more monosaccharides to compensate for a lower sucrose content or more sucrose to compensate for a lower monosaccharide content. The relationship between DW and AIS was similar (AIS = 0.00089 × DW), and DW and AIS increased with time for most cultivars. Cultivars with high DW and AIS can be selected early during storage root development.
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Simmons, Donna M. "Cryoprotectant Tissue Storage Solutions: Stability at Lower Temperatures, Longer Storage Times, More Versatile Usage." Microscopy Today 8, no. 3 (April 2000): 28. http://dx.doi.org/10.1017/s155192950006106x.
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Infusion with buffer/sucrose solutions (up to 30% sucrose) has long been used to ‘cryoprotect’ tissue in an attempt to prevent ice crystal artifact in frozen sections. This is helpful for example in sectioning large, fixed tissue blocks that must be frozen relatively slowly in dry ice to allow sectioning on a sliding microtome. In 1977, De Olmos added ethylene glycol to the mixture (30 g cane sugar/50 ml 0.1 M PO4 buffer at pH 7.2 in 20 ml ethylene glycoi) for -10°C storage of free floating sections from lightly fixed primate brain. Jones and Kane in 78 used this solution for storage of sections at -20°C (standard household freezer temperature) for up to one month before horseradish peroxidase histochemical reaction, in their methods, they cautioned that "sucrose attracts insects" (ants, personal communication). We and others have found the above cryoprotectant solution generally useful for storage of free floating sections from fixed brain. We observed that adjacent sections stored at 4°C in buffer for 2 weeks (common practice for Nissl stained sections) lost their reactivity to antibody labelling.
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Bowtell, J. L., K. Gelly, M. L. Jackman, A. Patel, M. Simeoni, and M. J. Rennie. "Effect of different carbohydrate drinks on whole body carbohydrate storage after exhaustive exercise." Journal of Applied Physiology 88, no. 5 (May 1, 2000): 1529–36. http://dx.doi.org/10.1152/jappl.2000.88.5.1529.
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Seven untrained male subjects participated in a double-blind, crossover study conducted to determine the efficacy of different carbohydrate drinks in promoting carbohydrate storage in the whole body and skeletal muscle during recovery from exhaustive exercise. The postabsorptive subjects first completed an exercise protocol designed to deplete muscle fibers of glycogen, then consumed 330 ml of one of three carbohydrate drinks (18.5% glucose polymer, 18.5% sucrose, or 12% sucrose; wt/vol) and also received a primed constant infusion of [1-13C]glucose for 2 h. Nonoxidative glucose disposal (3.51 ± 0.28, 18.5% glucose polymer; 2.96 ± 0.32, 18.5% sucrose; 2.97 ± 0.16, 12% sucrose; all mmol ⋅ kg− 1 ⋅ h− 1) and storage of muscle glycogen (5.31 ± 1.11, 18.5% glucose polymer; 4.07 ± 1.05, 18.5% sucrose; 3.45 ± 0.85, 12% sucrose; all mmol ⋅ kg wet wt−1 ⋅ h− 1; P < 0.05) were greater after consumption of the glucose polymer drink than after either sucrose drink. The results suggest that the consumption of a glucose polymer drink (containing 61 g carbohydrate) promotes a more rapid storage of carbohydrate in the whole body, skeletal muscle in particular, than an isoenergetic sucrose drink.
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Walsh, Kerry B., Russell C. Sky, and Sharon M. Brown. "The anatomy of the pathway of sucrose unloading within the sugarcane stalk." Functional Plant Biology 32, no. 4 (2005): 367. http://dx.doi.org/10.1071/fp04102.
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The physical path of sucrose unloading in the sugarcane stalk is described. About 50% of the vascular bundles in the internodes were located within 3 mm of the outside of the stalk. These bundles were inactive in long distance sucrose transport, as assessed by dye tracers of phloem flow. A sheath of fibres isolates the phloem apoplast from that of the storage parenchyma. In bundles associated with long distance transport (i.e. in the central region), the fibre sheath is narrowest to either side of the phloem fibre cap, and consists of living cells with plasmodesmata within pits in the secondary wall. Plasmodesmata were also arranged into pit fields between cells of the storage parenchyma. Since the vascular apoplast is isolated from the apoplast of the storage parenchyma, sucrose must move through the symplast of the fibre sheath. The calculated flux of sucrose through plasmodesmata of this cell layer was at the low end of reported values in the literature. Sucrose unloading within the storage parenchyma may also follow a symplastic route, with unloading into the apoplast of the storage parenchyma occurring as part of a turgor mechanism to increase sink strength.
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Fugate, Karen K., John D. Eide, Daniel N. Martins, Michael A. Grusak, Edward L. Deckard, and Fernando L. Finger. "Colocalization of sucrose synthase expression and sucrose storage in the sugarbeet taproot indicates a potential role for sucrose catabolism in sucrose accumulation." Journal of Plant Physiology 240 (September 2019): 153016. http://dx.doi.org/10.1016/j.jplph.2019.153016.
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Davis, Jeanine M., and Wayne H. Loescher. "Diurnal Pattern of Carbohydrates in Celery Leaves of Various Ages." HortScience 26, no. 11 (November 1991): 1404–6. http://dx.doi.org/10.21273/hortsci.26.11.1404.
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Diurnal fluctuations in soluble carbohydrates and starch were monitored in young (expanding), mature (first fully expanded), and old (nearing senescence) celery (Apium graveolens L.) leaves. In all tissues, mannitol and sucrose were the carbohydrates present in the highest concentrations. In old and young leaflets and their petioles, there was little change in levels of mannitol and sucrose in 26 hours. In mature leaflets, sucrose accumulated in the light and decreased in the dark; mannitol increased slightly in late afternoon. Starch concentration, although quite low, showed definite diurnal fluctuations in mature leaflets, but only small changes in young and old leaflets. Both sucrose and mannitol were present in mature petiole phloem tissues. Mannitol concentrations were high in the adjacent storage parenchyma tissue, but sucrose was almost undetectable. These data support earlier findings that sucrose is produced, translocated, and metabolized throughout the celery plant. Mannitol is also translocated, but also serves as a major storage carbohydrate in leaf tissues, especially petiole parenchyma. Starch serves as a minor short-term storage compound in leaflets.
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Milkevych, V. "REDUCTION OF SUCROSE LOSSES DURING SUGAR BEET STORAGE." Scientific Works of National University of Food Technologies 25, no. 3 (June 2019): 206–14. http://dx.doi.org/10.24263/2225-2924-2019-25-3-24.
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Yu, Fang, Zhiming Ni, Xingfeng Shao, Lina Yu, Hongxing Liu, Feng Xu, and Hongfei Wang. "Differences in Sucrose Metabolism in Peach Fruit Stored at Chilling Stress versus Nonchilling Stress Temperatures." HortScience 50, no. 10 (October 2015): 1542–48. http://dx.doi.org/10.21273/hortsci.50.10.1542.
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To explore differences in sucrose metabolism between peach fruit subjected to chilling stress (5 °C) and nonchilling stress (10 °C), sucrose concentration as well as the activities and gene expression levels for enzymes associated with sucrose metabolism were compared. Fruits stored at 5 °C accumulated higher concentrations of H2O2 and developed severe chilling injury (CI) compared with fruit kept at 10 °C. Activities and gene expression levels for enzymes related to sucrose metabolism, such as acid invertase (AI), neutral invertase (NI), sucrose synthase (SS), and sucrose phosphate synthase (SPS) were higher in fruit stored at 5 °C than at 10 °C throughout or late in storage. A sharp increase in net sucrose cleavage activity dramatically decreased sucrose concentration and increased reducing sugars at 5 °C. The sucrose concentration at 10 °C increased over the first 21 days and then declined slightly, and was higher than in fruit at 5 °C throughout storage. The increase in net sucrose cleavage activity at 5 °C is contrary to the expectation that biochemical reactions ordinarily proceed more rapidly with increasing temperature. We conclude that chilling stress stimulates the activities and transcription levels of enzymes involved in sucrose metabolism, resulting in increased sucrose cleavage.
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Siswoyo, Tri Agus, Ika Oktavianawati, Djenal Djenal, Bambang Sugiharto, and Untung Murdiyanto. "CHANGES OF SUCROSE CONTENT AND INVERTASE ACTIVITY DURING SUGARCANE STEM STORAGE." Indonesian Journal of Agricultural Science 8, no. 2 (October 25, 2016): 75. http://dx.doi.org/10.21082/ijas.v8n2.2007.75-81.
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Invertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the key enzymes involved in sucrose metabolism in sugarcane plants. They are highly correlated with sucrose and reducing sugar contents during plant growth. The sugarcane plants have two kinds of invertases, namely neutral invertase (NI) and acid invertase (AI). They have different function in sucrose accumulation. The research aimed to study the role of AI and NI in accumulation of reducing sugar during storage of sugarcane stems. Plant materials of 18-month-old field grown sugarcane of the commercial variety R-579 (wet-land) and M 442-51 (dry-land) were used. Three internodes were sampled to represent immature (internode 1-8/F1), maturing (internode 9-16/F2), and mature (internode 17-24/F3) stem tissues. All tissues were stored for 0-9 days at room temperature (28-32oC) and each day, the sample<br />was extracted to determine invertase activity, total soluble protein, and sugar contents. This observation was valid for invertase activity expressed on a protein basis. At the initiate<br />harvested (0-3 days), NI had a higher specific activity than AI in the sucrose-accumulating region of the sugarcane stems. Negative significant correlation was found between NI specific activity and sucrose accumulation (r2 = 0.41, P < 0.05). AI showed a higher specific activity after 4 days harvested and had negative correlation with sucrose accumulation (r2 = 0.40, P < 0.05). These results showed that NI could be more responsible in sucrose hydrolisis than AI at early storage of sugarcane stems.
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Siswoyo, Tri Agus, Ika Oktavianawati, Djenal Djenal, Bambang Sugiharto, and Untung Murdiyanto. "CHANGES OF SUCROSE CONTENT AND INVERTASE ACTIVITY DURING SUGARCANE STEM STORAGE." Indonesian Journal of Agricultural Science 8, no. 2 (October 25, 2016): 75. http://dx.doi.org/10.21082/ijas.v8n2.2007.p75-81.
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Invertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the key enzymes involved in sucrose metabolism in sugarcane plants. They are highly correlated with sucrose and reducing sugar contents during plant growth. The sugarcane plants have two kinds of invertases, namely neutral invertase (NI) and acid invertase (AI). They have different function in sucrose accumulation. The research aimed to study the role of AI and NI in accumulation of reducing sugar during storage of sugarcane stems. Plant materials of 18-month-old field grown sugarcane of the commercial variety R-579 (wet-land) and M 442-51 (dry-land) were used. Three internodes were sampled to represent immature (internode 1-8/F1), maturing (internode 9-16/F2), and mature (internode 17-24/F3) stem tissues. All tissues were stored for 0-9 days at room temperature (28-32oC) and each day, the sample<br />was extracted to determine invertase activity, total soluble protein, and sugar contents. This observation was valid for invertase activity expressed on a protein basis. At the initiate<br />harvested (0-3 days), NI had a higher specific activity than AI in the sucrose-accumulating region of the sugarcane stems. Negative significant correlation was found between NI specific activity and sucrose accumulation (r2 = 0.41, P < 0.05). AI showed a higher specific activity after 4 days harvested and had negative correlation with sucrose accumulation (r2 = 0.40, P < 0.05). These results showed that NI could be more responsible in sucrose hydrolisis than AI at early storage of sugarcane stems.
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Silva, Silvanda, Sven Verlinden, Robert Herner, and Randolph Beaudry. "Sugars and Respiration Profiles in Asparagus Spears and Changes during Storage." HortScience 30, no. 4 (July 1995): 823F—823. http://dx.doi.org/10.21273/hortsci.30.4.823f.
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Base-to-tip profiles of sucrose, glucose, fructose, and respiration rate were measured for asparagus (Asparagus officinalis L.) spears stored at 0C. Fructose content was ≈3-fold and 4-fold higher than glucose and sucrose, respectively. The highest level of fructose was found in the base and was ≈15-fold higher than the tip. The changes in asparagus metabolism were characterized by loss of sucrose and a high rate of respiration within the first hours after harvest. Sucrose was more rapidly lost than the other sugars during this period. The respiration rate was measured along the length of intact spears at 0.5, 1, 2, and 3 h after harvest. Subsequent measurements were taken after larger time intervals for 23 days. The respiration rate declined rapidly to ≈60% of the initial rate within 12 h, decreasing more slowly thereafter. Initially, the respiration rate of the tip was about four times that of the base, but, after 23 days, the respiration rate of the tip was only twice that of the base. Sucrose content and respiration rates were closely correlated.
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Dewi, Eko Nurcahya, Aulleta Affri Nurbaiti, and Lukita Purnamayati. "Chemical Changes of Shredded Catfish (Clarias gariepinus) Added with Different Concentration of Sucrose During Storage at Room Temperature." E3S Web of Conferences 147 (2020): 03001. http://dx.doi.org/10.1051/e3sconf/202014703001.
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Shredded catfish is a processed dried food, usually used as a side dish and stored for a long time. The main criteria of shredded fish selection is its taste, color and appearance. During storage, shredded catfish might be experienced deterioration in quality. Therefore, the purpose of this study was to determine changes in the chemical quality of shredded catfish with different sucrose concentrations during storage. The study was carried out by adding sucrose to the formulation of shredded catfish respectively 27.5%, 32.5%, and 37.5%. For a comparison purposed, commercial shredded catfish with sucrose addition of 35% was applied. All samples were stored for 60 days and observed every 30 days for the moisture content, aw, color measurement, peroxide value (PV), thiobarbituric acid (TBA), free fatty acid (FFA) and fatty acid profile. The results show that during storage at room temperature shredded catfish with 27.5% sucrose was the best quality product. During storage there was an increase in moisture content, aw, TBA and FFA, while for PV parameters increased in fluctuation at day 30 of storage and decreased at day 60 of storage. Color parameter decreased from dark to slightly bright and unsaturated fatty acids decreased in oleic, linoleic, linolenic and DHA. Based on these results, shredded catfish still safe to be consumed during storage for 60 days or two months.
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Klotz, Karen L., and Larry G. Campbell. "Effects of Aphanomyces Root Rot on Carbohydrate Impurities and Sucrose Extractability in Postharvest Sugar Beet." Plant Disease 93, no. 1 (January 2009): 94–99. http://dx.doi.org/10.1094/pdis-93-1-0094.
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Sugar beet (Beta vulgaris) roots with rot caused by Aphanomyces cochlioides often are incorporated into storage piles even though effects of disease on processing properties are unknown. Roots with Aphanomyces root rot were harvested from six fields over 2 years. For each field, roots with similar disease symptoms were combined and assigned a root rot index (RRI) value (0 to 100; 0, no rot symptoms; 100, all roots severely rotted). After 20 or 120 days storage at 4°C and 95% relative humidity, concentrations of the major carbohydrate impurities that accumulate during storage and sucrose extractability were determined. Root rot affected carbohydrate impurity concentrations and sucrose extractability in direct relation to disease severity symptoms. Generally, roots with active and severe infection (RRI ≥ 85) exhibited elevated glucose and fructose concentrations 20 and 120 days after harvest (DAH), elevated raffinose concentration 120 DAH, and reduced sucrose extractability 20 and 120 DAH. Roots with minor or moderate disease symptoms (RRI 20 to 69), or damaged roots with no signs of active infection, had similar carbohydrate impurity concentrations and sucrose extractability after 20 and 120 days storage. Processing properties declined when RRIs exceeded 43, as determined by regression analysis, or when storage duration increased from 20 to 120 days. Results indicate that both disease severity and anticipated duration of storage be considered before Aphanomyces-infected roots are incorporated into storage piles.
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Hussain, Syed Bilal, Cai-Yun Shi, Ling-Xia Guo, Wei Du, Ying-Xing Bai, Hafiz Muhammad Kamran, Alisdair R. Fernie, and Yong-Zhong Liu. "Type I H+-pyrophosphatase regulates the vacuolar storage of sucrose in citrus fruit." Journal of Experimental Botany 71, no. 19 (June 26, 2020): 5935–47. http://dx.doi.org/10.1093/jxb/eraa298.
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Abstract The aim of this work was to evaluate the general role of the vacuolar pyrophosphatase proton pump (V-PPase) in sucrose accumulation in citrus species. First, three citrus V-PPase genes, designated CsVPP-1, CsVPP-2, and CsVPP-4, were identified in the citrus genome. CsVPP-1 and CsVPP-2 belonging to citrus type I V-PPase genes are targeted to the tonoplast, and CsVPP-4 belonging to citrus type II V-PPase genes is located in the Golgi bodies. Moreover, there was a significantly positive correlation between transcript levels of type I V-PPase genes and sucrose, rather than hexose, content in fruits of seven citrus cultivars. Drought and abscisic acid treatments significantly induced the CsVPP-1 and CsVPP-2 transcript levels, as well as the sucrose content. The overexpression of type I V-PPase genes significantly increased PPase activity, decreased pyrophosphate contents, and increased sucrose contents, whereas V-PPase inhibition produced the opposite effect in both citrus fruits and leaves. Furthermore, altering the expression levels of type I V-PPase genes significantly influenced the transcript levels of sucrose transporter genes. Taken together, this study demonstrated that CsVPP-1 and CsVPP-2 play key roles in sucrose storage in the vacuole by regulating pyrophosphate homeostasis, ultimately the sucrose biosynthesis and transcript levels of sucrose transport genes, providing a novel lead for engineering or breeding modified taste in citrus and other fruits.
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Aggarwal, P., and M. Michael. "Effect of replacing sucrose with fructose on the physico-chemical sensory characteristics of kinnow candy." Czech Journal of Food Sciences 32, No. 2 (April 22, 2014): 158–63. http://dx.doi.org/10.17221/221/2013-cjfs.
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We determine the effect of substitution of sucrose with fructose on the physico-chemical composition and sensory characteristics of kinnow candy. Candy was prepared with peel using sucrose and fructose at the ratio of 100:0, 0:100, 75:25, 50:50, and 25:75 with 70 °B of TSS. Organoleptically, the candy prepared with 100% fructose proved the best but the candy prepared with 25:75 of sucrose to fructose was equally good. The least acceptable was the candy prepared with 100% sucrose, as it had a dull appearance and slight crystallisation was observed during storage. Moisture, acidity and ascorbic acid decreased while total soluble solids, reducing and total sugars and limonin increased with no change in ash content of the candies during four months of storage.
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Chenlo, F., R. Moreira, L. Chaguri, and M. D. Torres. "Note. Sugar, Moisture Contents, and Color of Chestnuts during Different Storage Regimes." Food Science and Technology International 15, no. 2 (April 2009): 169–78. http://dx.doi.org/10.1177/1082013208106321.
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Color, moisture and sucrose, glucose and fructose contents of chestnuts (Castanea sativa Mill.) at several initial states (whole and undried; whole, peeled, and partially air-dried, with or without prior sucrose treatment; peeled, broken, and undried or partially air-dried) were monitored for 11 months during storage under various conditions (unpacked, packed in plastic bags at ambient pressure, or vacuum packed) and temperature (at room temperature, 3°C or -18°C). Moisture content decreased considerably in all cases except in those of packed whole peeled chestnuts without sucrose treatment and stored at room temperature or 3°C. Chestnuts stored for 2 months in contact with the atmosphere had moisture contents of only ∼4% of their dry weight. After 4 months storage, sucrose content had fallen (in some cases to zero) in all samples except in broken chestnuts dried to a 2% moisture content and conventionally stored whole unpeeled chestnuts (in which sucrose content rose slightly). Glucose and fructose contents generally peaked after 1-2 months of storage, and by month 4 returned to initial or lower levels. Sugar content was hardly affected by initial moisture or sugar contents except when moisture content had been reduced to 2%. Color changes were least during storage in air or at 3°C.
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Silva, Silvanda, Sven Verlinden, Robert Herner, and Randolph Beaudry. "Effects of Carbohydrate Loading on Sugar Profile and Respiration of Asparagus Spears during Storage." HortScience 30, no. 4 (July 1995): 824F—824. http://dx.doi.org/10.21273/hortsci.30.4.824f.
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Asparagus spears (Asparagus officinalis L.) were placed in solutions of six different concentrations of sucrose (0%, 1%, 2%, 4%, 8%, and 16%) plus citric acid at 0C for 24 h following harvest. The profiles of sucrose, fructose, glucose, and respiration rate along the length of the spear were evaluated throughout storage. The effect of carbohydrate loading on the rate of respiration, sucrose loss, and the shelf life of asparagus was determined. For all treatments, sugars decreased and respiration rate increased from the butt to the tip. The 4% sucrose treatment enhanced the sugar level in the tip ≈5-fold relative to the control. For the 8% and 16% treatments, sucrose tended to accumulate in the base. Spears loaded with higher sucrose concentrations had higher respiration rates than controls up to 3 h after loading. After this time, no significant differences were observed between treatments. For all treatments, respiration rates declined rapidly following harvest, stabilizing in ≈24 h. Weight gain and growth increased as the treatment sucrose concentration decreased. Solution uptake was enhanced by loading at lower humidity levels.
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Bernal-Lugo, I., and A. C. Leopold. "Seed stability during storage: Raffinose content and seed glassy state." Seed Science Research 5, no. 2 (June 1995): 75–80. http://dx.doi.org/10.1017/s0960258500002646.
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AbstractIt has been proposed that sucrose and raffinose play a role in the storability of maize seeds. The levels of these sugars in the embryos and the glassy state were compared in maize seeds of contrasting storage stability to determine the relationship between sugar composition, glassy state and the storability of the seed. Sucrose was the predominant sugar but its content was not correlated with good storage. The content of raffinose as a mass fraction of total sugars and the magnitude of the glassy state showed positive correlations with storage stability. It is suggested that in maize seeds dry storage stability is a reflection not of the total soluble sugars, but of the mixture of sucrose with raffinose. Apparently raffinose amplifies the magnitude of the glass signal and this in turn is associated with enhanced storability.
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Guo, Jianmin, William A. Jermyn, and Matthew H. Turnbull. "Carbon partitioning and sucrose metabolism in two field-grown asparagus (Asparagus officinalis) cultivars with contrasting yield." Functional Plant Biology 29, no. 4 (2002): 517. http://dx.doi.org/10.1071/pp01148.
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The aim of this study was to investigate the roles of carbon partitioning and sucrose metabolism in regulating cultivar differences in spear yield in asparagus (Asparagus officinalis L.). In the two cultivars studied, maximum net photosynthetic rate (Amax) was positively correlated with sucrose phosphate synthase (SPS) activity (r2=0.86), which was in turn linked to increases in sucrose content in cladophyll tissue. The high-yielding cultivar ASP-69 exhibited greater SPS activity and sucrose content than the low-yielding cultivar ASP-03, in fully-expanded and mature cladophyll tissue. ASP-69 also displayed a higher percentage of soluble solids in stem cell sap than did ASP-03. Sucrose synthase (SS) activity in storage roots in ASP-69 was significantly greater than in ASP-03 during fern growth season. Total non-structural carbohydrate (TNC) in storage roots did not differ in the two cultivars. Biomass analysis revealed that ASP-69 had a greater root/shoot ratio than ASP-03, suggesting that the total carbohydrate storage pool, rather than carbohydrate concentration, is an important determinant of asparagus yield. The overall results substantiate the conclusion that carbohydrate partitioning in the two asparagus cultivars studied is a property of the entire plant, and is influenced by both source and sink properties. This is highlighted by greater Amax, SPS activity and sucrose concentrations in cladophyll tissue in ASP-69, and greater SS activity and total carbohydrate content in storage root tissue in ASP-69.
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Thammawong, Manasikan, Daisuke Nei, Poritosh Roy, Nobutaka Nakamura, Takeo Shiina, Yuuichi Inoue, Hidenobu Hamachi, and Shigeyuki Nonaka. "Characteristics of Sugar Content in Different Sections and Harvest Maturity of Bamboo Shoots." HortScience 44, no. 7 (December 2009): 1941–46. http://dx.doi.org/10.21273/hortsci.44.7.1941.
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Bamboo shoots (Phyllostachys pubescens Mazel) harvested from two cultivation areas were used to investigate the amount of accumulated sugars (sucrose, glucose, and fructose) in four sections along the length of a bamboo shoot. Bamboo shoots harvested from above ground (emerged bamboo shoot) and underground of the same cultivation area were also used to study the changes in sugar content during storage at both 5 and 25 °C. The amounts of sucrose, glucose, fructose, and total sugar of underground bamboo shoots were higher than those of emerged shoots. Sucrose content in the apical section was significantly higher than that in other sections. Meanwhile, higher amounts of glucose, fructose, and total sugar (the sum of sucrose, glucose, and fructose) were observed in the basal section. Changes in sugar content were also observed during storage. The fresh, unpeeled bamboo shoots have particular cellular chemical properties and respond differently to storage duration and condition depending on harvest maturity.
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Tegeder, Mechthild, Melinda Thomas, Louise Hetherington, Xin-Ding Wang, Christina E. Offler, and John W. Patrick. "Genotypic differences in seed growth rates of Phaseolus vulgaris L. II. Factors contributing to cotyledon sink activity and sink size." Functional Plant Biology 27, no. 2 (2000): 119. http://dx.doi.org/10.1071/pp99117.
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A previous study [Thomas et al. (2000) Aust. J. Plant Physiol. 27, 109–118] showed that genotypic dif-ferences in seed growth rates of Phaseolus vulgaris L. cultivars was accounted for by variation in dry matter flux and seed size. Bulk cotyledon saps contained identical concentrations of sucrose across cultivars suggesting that geno-typic variation in capacities for sucrose transport and metabolism are equally matched. Cotyledon sucrose transport, monitored as in vitro uptake of [14C]sucrose, exhibited genotypic variation and this was abolished by para-chloromercuribenzene- sulfonate. Eadie–Hofstee transformations of concentration-dependent [14C]sucrose uptake showed that genotypic variation in sucrose flux resulted from differences in maximal transporter activity. Maximal sucrose fluxes and levels of transcript and microsomal protein for the sucrose/H+ symporter and H+-ATPase were positively correlated. In contrast, sucrose binding protein transcript and microsomal protein levels correlated negatively with sucrose fluxes. In all cultivars, a sucrose/H+ symporter and H+-ATPase were co-localised to plasma membranes of the dermal cell complexes. Total plasma membrane surface areas of the dermal cell complexes and total volume of storage parenchyma cells correlated with cultivar variation in seed growth rates. Differences in cell number and size accounted for cultivar variation in total plasma membrane surface area of the dermal cell complexes and total storage parenchyma cell volume.
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Rizal, Samsul, Julfi Restu Amelia, and Suharyono A. S. "THE EFFECT OF ADDITION OF SUCROSE SOLUTION ON THE ANTIBACTERIAL ACTIVITIES OF GREEN GRASS JELLY EXTRACT SINBIOTIC BEVERAGES DURING STORAGE IN COLD TEMPERATURE." Agric 31, no. 1 (July 21, 2019): 53–66. http://dx.doi.org/10.24246/agric.2019.v31.i1.p53-66.
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Sinbiotic drinks have a very acidic taste, so it is necessary to add sucrose solution to get the best taste. This study aims to determine the effect of adding 65% (v/v) sucrose solution to changes in antibacterial activity of green grass jelly synbiotic drinks during storage in cold temperatures. The finished green grass jelly synbiotic product was given two different treatments, namely the product without the addition of sucrose solution and product with the addition of 10% (v/v) of 65% (b/v) sucrose solution. The product was stored for 28 days at a cold temperature of ± 10oC. Observations were carried out every 7 days for antibacterial activity, pH, total acid, and total lactic acid bacteria. Antibacterial activity was evaluated using the agar diffusion method against pathogenic bacteria including Staphylococcus aureus, Salmonella sp., Bacillus cereus, and Eschericia coli. The results showed that the antibacterial activity, pH, and total lactic acid bacteria of green grass jelly synbiotic drinks both without and with the addition of 65% (b/v) sucrose as much as 10% (v/v) reduced during storage at cold temperatures, while total acid increases. There was no significant difference between the antibacterial activity and the characteristics of the green grass jelly synbiotic drink given 65% sucrose solution and without the addition of 65% sucrose solution. Thus the study concluded that the addition of 65% sucrose solution to increase the preference for the product did not significantly affect the change in antibacterial activity of the green grass jelly synbiotic beverage during storage in cold temperatures.
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Liu, Juan, Qinghua Huang, Peizi Kang, Lei Liang, and Junjia Chen. "Lignin Accumulation in Three Pumelo Cultivars in Association with Sucrose and Energy Depletion." Biomolecules 9, no. 11 (November 5, 2019): 701. http://dx.doi.org/10.3390/biom9110701.
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Lignification, which occurs in many horticultural fruit and vegetables, brings about undesirable texture and unfavorable consumer preference. However, this problem has rarely been studied. In this work, three pumelo cultivars cvs “Hongroumiyou” (HR), “Bairoumiyou” (BR), and “Huangroumiyou” (HuR) were stored at 25 °C for 90 days, and juice sacs were sampled to explore the lignin accumulation and its relationship to sucrose and energy depletion were investigated. The results displayed that HuR contained lower sucrose content, lower ATP level, but higher lignin content compared to BR and HR during postharvest storage, indicating that the sequence according to storage resistance on the basis of lignin content is as follows: HuR < BR < HR. Furthermore, sucrose degradation attributed to enhanced activities of neutral invertase (NI), soluble acid invertase (S-AI), cell wall-bound invertase (B-AI), and energy deficit on account of declined ATP level, showed significantly negative correlation with lignin accumulation, suggesting that lignin accumulation occurrence could induce sucrose degradation and energy deficit during postharvest storage. Additionally, higher activities of phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD) could accelerate lignin synthesis and resulted in lignin accumulation during postharvest pumelo storage.
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Hill, L. M., and S. Rawsthorne. "Carbon supply for storage-product synthesis in developing seeds of oilseed rape." Biochemical Society Transactions 28, no. 6 (December 1, 2000): 667–69. http://dx.doi.org/10.1042/bst0280667.
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The aim of this work was to find out how the sugars in the endosperm of oilseed rape contribute to the flux of oil synthesis. While the hexose content of the liquid endosperm decreased during development the sucrose content increased. It is important to understand the relative rates of use of the endosperm sugars for two reasons. Firstly we need to know which sugars are used, and at what stages in development, in order to understand the roles of enzymes involved in their metabolism. Secondly, changes in sugar concentration have been implicated in the regulation of expression of genes determining storage-product synthesis [see Weber, Borisjuk and Wobus (1997) Trends Plant Sci. 2, 169–174, for review]. The rate of consumption of sugar is one factor governing its concentration. We present data showing both the concentration-dependence of conversion of sugar to oil, and the in vivo concentrations of sugars; we relate these data sets to each other and discuss the effects of the intracellular pool of sucrose. Glucose, fructose and sucrose are all substrates for oil synthesis, but the rates of their use (particularly sucrose) are underestimated because of dilution by sucrose from the intracellular pool.
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Babić, J., D. Šubarić, B. Milicevic, D. Ačkar, M. Kopjar, and N. Nedic Tiban. "Influence of trehalose, glucose, fructose, and sucrose on gelatinisation and retrogradation of corn and tapioca starches." Czech Journal of Food Sciences 27, No. 3 (July 27, 2009): 151–57. http://dx.doi.org/10.17221/31/2009-cjfs.
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The effects of trehalose, glucose, fructose, and sucrose on the gelatinisation and retrogradation properties of corn (CS) and tapioca (TS) starches were studied with differential scanning calorimetry (DSC). The results showed that the sugars affect gelatinisation and retrogradation of both starches, with the effect varying significantly between sugars. The addition of trehalose, glucose, fructose, and sucrose increased the gelatinisation temperatures and enthalpy of gelatinisation of corn and tapioca starches. The extent of increase followed the order: fructose < glucose < trehalose < sucrose with CS, and fructose < trehalose < sucrose < glucose with TS. The retrogradation studies showed that sugars of lower molecular weights (glucose and fructose) were less effective in the reduction of retrogradation than those of higher molecular weights (sucrose and trehalose). Trehalose retarded retrogradation of both corn and tapioca starches under all conditions investigated. Sucrose had the same effect on the corn starch retrogradation. The effects of other sugars depended on the type of starch, storage period, and storage temperature.
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Weber, Alison, and Sharareh Hekmat. "The Effect of Stevia rebaudiana on the Growth and Survival of Lactobacillus rhamnosus GR-1 and Sensory Properties of Probiotic Yogurt." Journal of Food Research 2, no. 2 (March 28, 2013): 136. http://dx.doi.org/10.5539/jfr.v2n2p136.
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<span style="font-family: Times New Roman; font-size: small;"></span><strong><span style="font-family: "Times New Roman","serif"; font-size: 10pt;" lang="EN-US"></span></strong>This study evaluated the effect of various sweetening agents on sensory properties of probiotic yogurt. The growth and survival of <em>Lactobacillus rhamnosus</em><em> </em>GR-1 in yogurt was measured at 1, 14 and 28 days of storage. Bacterial growth reached a range of 10<sup>8</sup>-10<sup>9</sup> Colony Forming Units (CFU) ml<sup>-1</sup> over the storage period for six probiotic yogurt samples (0.12% stevia, 0.12% stevia-inulin-chromium (SIC), 1.0% sucralose, 5.0% sucrose, 6.0% xylitol and a control). Sensory panelists (n = 109) used a 9-point hedonic scale and evaluated five sensory characteristics (flavour, appearance, sweetness, texture, and overall quality) of the four sweetened probiotic yogurts (0.12% stevia, 0.12% SIC, 1.0% sucralose and 5.0% sucrose). The sucralose sample was significantly preferred for all five characteristics (P < 0.001) and the sucrose sample was significantly liked more than both the stevia and SIC samples for all characteristics except flavour (P < 0.001). Overall, the stevia sample received higher mean scores than the SIC sample.<span style="font-family: Times New Roman; font-size: small;"> </span>
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Campbell, L. G., K. L. Klotz, and L. J. Smith. "Postharvest Storage Losses Associated with Rhizomania in Sugar Beet." Plant Disease 92, no. 4 (April 2008): 575–80. http://dx.doi.org/10.1094/pdis-92-4-0575.
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During storage of sugar beet, respiration and rots consume sucrose and produce invert sugar. Diseases that occur in the field can affect the magnitude of these losses. This research examines the storage of roots with rhizomania (caused by Beet necrotic yellow vein virus) and the effectiveness of rhizomania-resistant hybrids in reducing postharvest losses. Roots of susceptible hybrids from sites with rhizomania had respiration rates 30 days after harvest (DAH) that ranged from 0.68 to 2.79 mg of CO2 kg–1 h–1 higher than roots of the resistant hybrids. This difference ranged from 2.60 to 13.88 mg of CO2 kg–1 h–1 120 DAH. Roots of resistant hybrids from sites with rhizomania had 18 kg more sucrose per ton than roots from susceptible hybrids 30 DAH, with this difference increasing to 55 kg Mg–1 120 DAH. The invert sugar concentration of susceptible hybrids from sites with rhizomania ranged from 8.38 to 287 g per 100 g of sucrose higher than that for resistant hybrids 120 DAH. In contrast, differences between susceptible and resistant hybrids in respiration rate, sucrose loss, and invert sugar concentration in the absence of rhizomania were relatively small. Storage losses due to rhizomania can be minimized by planting resistant hybrids and processing roots from fields with rhizomania soon after harvest.
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Tarpley, Lee, Donald M. Vietor, and Frederick R. Miller. "Metabolism of Sucrose During Storage in Intact Sorghum Stalk." International Journal of Plant Sciences 157, no. 2 (March 1996): 159–63. http://dx.doi.org/10.1086/297334.
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Echeverria, Ed. "Intracellular localization of sucrose-phosphate phosphatase in storage cells." Physiologia Plantarum 95, no. 4 (December 1995): 559–62. http://dx.doi.org/10.1034/j.1399-3054.1995.950408.x.
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Echeverria, Ed. "Intracellular localization of sucrose-phosphate phosphatase in storage cells." Physiologia Plantarum 95, no. 4 (December 1995): 559–62. http://dx.doi.org/10.1111/j.1399-3054.1995.tb05522.x.
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Beuchat, Larry R., David A. Mann, Christine A. Kelly, and Ynes R. Ortega. "Retention of Viability of Salmonella in Sucrose as Affected by Type of Inoculum, Water Activity, and Storage Temperature." Journal of Food Protection 80, no. 9 (July 25, 2017): 1408–14. http://dx.doi.org/10.4315/0362-028x.jfp-16-537.
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ABSTRACT Outbreaks of salmonellosis have been associated with consumption of high-sugar, low–water activity (aw) foods. The study reported here was focused on determining the effect of storage temperature (5 and 25°C) on survival of initially high and low levels of Salmonella in dry-inoculated sucrose (aw 0.26 ± 0.01 to 0.54 ± 0.01) and wet-inoculated sucrose (aw 0.24 ± 0.01 to 0.44 ± 0.04) over a 52-week period. With the exception of dry-inoculated sucrose at aw 0.26, Salmonella survived for 52 weeks in dry- and wet-inoculated sucrose stored at 5 and 25°C. Retention of viability was clearly favored in sucrose stored at 5°C compared with 25°C, regardless of level or type of inoculum or aw. Survival at 5°C was not affected by aw. Initial high-inoculum counts of 5.18 and 5.25 log CFU/g of dry-inoculated sucrose (aw 0.26 and 0.54, respectively) stored for 52 weeks at 5°C decreased by 0.56 and 0.53 log CFU/g; counts decreased by >4.18 and >4.25 log CFU/g in samples stored at 25°C. Inactivation rates in wet-inoculated sucrose were similar to those in dry-inoculated sucrose; however, a trend toward higher persistence of Salmonella in dry- versus wet-inoculated sucrose suggests there was a higher proportion of cells in the wet inoculum with low tolerance to osmotic stress. Survival patterns were similar in sucrose initially containing a low level of Salmonella (2.26 to 2.91 log CFU/g). The pathogen was recovered from low-inoculated sucrose stored at 5°C for 52 weeks regardless of type of inoculum or aw and from dry-inoculated sucrose (aw 0.54) and wet-inoculated sucrose (aw 0.24) stored at 25°C for 12 and 26 weeks, respectively. Results emphasize the importance of preventing contamination of sucrose intended for use as an ingredient in foods not subjected to a treatment that would be lethal to Salmonella.
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Chiewchankaset, Porntip, Saowalak Kalapanulak, and Treenut Saithong. "Extended Utilization of Constraint-Based Metabolic Model in a Long-Growing Crop." Processes 7, no. 5 (May 4, 2019): 259. http://dx.doi.org/10.3390/pr7050259.
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The constraint-based rMeCBM-KU50 model of cassava storage root growth was analyzed to evaluate its sensitivity, with respect to reaction flux distribution and storage root growth rate, to changes in model inputted data and constraints, including sucrose uptake rate-related data—photosynthetic rate, total leaf area, total photosynthetic rate, storage root dry weight, and biomass function-related data. These mainly varied within ±90% of the model default values, although exceptions were made for the carbohydrate (−90% to 8%) and starch (−90% to 9%) contents. The results indicated that the predicted storage root growth rate was highly affected by specific sucrose uptake rates through the total photosynthetic rate and storage root dry weight variations; whereas the carbon flux distribution, direction and partitioning inclusive, was more sensitive to the variation in biomass content, particularly the carbohydrate content. This study showed that the specific sucrose uptake rate based on the total photosynthetic rate, storage root dry weight, and carbohydrate content were critical to the constraint-based metabolic modeling and deepened our understanding of the input–output relationship—specifically regarding the rMeCBM-KU50 model—providing a valuable platform for the modeling of plant metabolic systems, especially long-growing crops.
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Pozueta-Romero, Diego, Pedro Gonzalez, Ed Etxeberria, and Javier Pozueta-Romero. "The Hyperbolic and Linear Phases of the Sucrose Accumulation Curve in Turnip Storage Cells Denote Carrier-mediated and Fluid Phase Endocytic Transport, Respectively." Journal of the American Society for Horticultural Science 133, no. 4 (July 2008): 612–18. http://dx.doi.org/10.21273/jashs.133.4.612.
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Sucrose accumulation into sink cells consists of multiple components collectively characterized by a biphasic kinetics curve. Whereas the hyperbolic phase at low external sucrose concentration denotes a membrane-bound, carrier-mediated component, the linear nonsaturable phase at higher concentrations has been suggested to represent facilitated diffusion. We recently demonstrated the occurrence of fluid phase endocytosis (FPE) uptake of sucrose in heterotrophic cells. To investigate the involvement of this process within both phases of the sucrose accumulation curve, we analyzed the effect of phloridzin and latrunculin-B (sucrose/H+ symport and endocytosis inhibitors, respectively) in the accumulation of sucrose and the endocytic marker Alexa-488 in turnip (Brassica campestris L.) storage parenchyma cells. At low external sucrose concentration, phloridzin, but not latrunculin-B, greatly reduced sucrose accumulation. By contrast, at high external sucrose concentration, phloridzin and latrunculin-B significantly inhibited sucrose accumulation. In addition, latrunculin-B exerted a pronounced inhibitory effect on Alexa-488 uptake at any external sucrose concentration. Our results indicate that carrier-mediated and endocytic uptake (at different magnitudes) of sucrose take place at any external sucrose concentration; that within the hyperbolic phase of the sucrose accumulation curve, most of the sucrose accumulating in the cell enters via plasmalemma-bound carrier(s); and that within the linear phase, plasmalemma-bound carriers and FPE are determinants of sucrose accumulation, with the involvement of FPE increasing parallel to external sucrose. Confocal laser scanning microscopy observations confirmed the increased involvement of FPE at higher external sucrose concentrations.
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Horsley, Tasmien N., Steven D. Johnson, and Terrence K. Stanger. "Optimising storage and in vitro germination of Eucalyptus pollen." Australian Journal of Botany 55, no. 1 (2007): 83. http://dx.doi.org/10.1071/bt05194.
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The best sucrose solution for maximum in vitro germination of Eucalyptus pollen was investigated in order to evaluate pollen germination rate as an indicator of pollen viability. In vitro germination of both freshly collected and 1-year-old pollen (stored at 4°C) of Eucalyptus grandis, E. smithii, E. nitens, E. dunnii and E. macarthurii was carried out in 0, 10, 20, 30, 40 and 50% (w/v) sucrose solutions, either with (0.15 mg L–1) or without boric acid. Similar trends were obtained for both fresh and 1-year-old pollen, with all species responding most favourably to 30% (w/v) sucrose and 0.15 mg L–1 boric acid. When an optimal in vitro germination medium had been established, the viabilities (%germination) of E. smithii, E. nitens and E. grandis pollen, stored at room (25°C), fridge (4°C), freezer (–10°C) and liquid nitrogen (–196°C) temperatures, were compared. For all tested species, germination declined as storage temperature increased, and by 8 months, the highest survival was obtained with cryostored pollen.
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Pasqual, Moacir, Leonardo Ferreira Dutra, Aparecida Gomes de Araujo, and Milene Alves de Figueiredo. "'Ponkan' mandarin (Citrus reticulata Blanco) immature fruits storage." Ciência e Agrotecnologia 30, no. 5 (October 2006): 1017–20. http://dx.doi.org/10.1590/s1413-70542006000500029.
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The aim of this work was to evaluate the effect of 'Ponkan' mandarin (C. reticulata) x 'Pêra' sweet orange (C. sinensis) immature fruits storage and sucrose concentrations on embryos in vitro culture. Fruits with 3 to 4 cm in diameter were harvested and placed inside black polyethylene bags with lateral openings and stored at 5±1ºC during 135 days. Every 15 days a sample was removed, its embryos were excised and individually inoculated in test tubes containing 15 mL of MS medium (Murashige & Skoog, 1962) with sucrose (0, 1.5, 3, 6, 12, 18 and 24 g L-1) and 0.3 mg L-1 GA3 and 1 g L-1 activated charcoal. Those treatments rested 48 hours in the dark and later in a growth room at 27 ± 1ºC with a 16-h photoperiod and 32 µmol m-2 s-1 light intensity. Immature fruits can be stored for posterior excision and embryos culture. Fruits with 120 days after the pollination can be stored for at most 135 days without damaging the embryos viability. It was observed a better development of the aerial part and root system of plantlets from 'Ponkan' mandarin x 'Pêra' sweet orange embryos in MS medium with 12-18 g L-1 sucrose.
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Diógenes Júnior, Armando Carlos, Stefanie De Freitas Almeida, Emanuel Neto Alves de Oliveira, Pedro Victor Crescêncio de Freitas, Bruno Fonsêca Feitosa, and Rosane Liége Alves de Souza. "Development and stability of candy in soursop mass." Ciência e Natura 42 (May 11, 2020): e12. http://dx.doi.org/10.5902/2179460x43168.
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The objective was to develop and characterize candies in soursop mass, replacing sucrose partially with glucose syrup, and to evaluate the stability during 90 days of storage under different temperatures. Two formulations of candies were prepared with sucrose substitution by glucose syrup, as well as a standard sample with sucrose alone. They were heated and concentrated to 71 °Brix for packaging in polyethylene packages. Afterwards, the candies were stored at 10 and 20 °C in a Biochemical Oxygen Demand (BOD) incubator and 28.1 °C (ambient temperature) for 90 days. During storage, the physical-chemical analyzes were performed: water content, total solids, pH, total titratable acidity, total soluble solids, water content and activity. It wasverified that the storage conditions caused reduction of the values of water content and water activity, besides increasing the values of total solids, total soluble solids and Ratio for all samples and storage conditions. The determining factor for the stability and preservation of product characteristics was the storage temperature; Being 10 ° C the ideal temperature for a better preservation of the candies in the standard formulation and 20 ° C for the added formulations of glucose syrup.
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Strausbaugh, Carl A., Imad Eujayl, Eugene Rearick, Paul Foote, and Dave Elison. "Sugar Beet Cultivar Evaluation for Storability and Rhizomania Resistance." Plant Disease 93, no. 6 (June 2009): 632–38. http://dx.doi.org/10.1094/pdis-93-6-0632.
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To reduce storage losses and improve resistance to rhizomania caused by Beet necrotic yellow vein virus (BNYVV), studies were initiated to establish a storage cultivar selection program. In 2006 and 2007, 30 or more commercial sugar beet (Beta vulgaris) cultivars were grown in soil naturally infested with BNYVV. At harvest, two root samples from each plot were collected and used to establish percent sugar. Additional samples were placed on top of an indoor pile (set point 1.7°C) and inside an outdoor pile in a randomized complete block design with four replications. After 142 and 159 days in indoor storage, sucrose reduction ranged from 13 to 90% in 2007 and 57 to 100% in 2008. Outdoor storage sucrose reduction ranged from 13 to 32% in 2007 and 28 to 60% in 2008. An average of 31 and 45% of the root surface was covered with fungal growth in 2007 and 2008, respectively. Cultivars that retained the most sucrose had resistance to BNYVV and the least fungal growth and weight loss. Indoor storage with BNYVV-infested roots allowed for the most consistent cultivar separation and will potentially lead to selection of cultivars for improved storability and rhizomania resistance.
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Blanchard, Mylène, Francois Castaigne, and Joseph Makhlouf. "CHANGES IN SUGAR CONTENT IN CUT ONION DURING STORAGE." HortScience 29, no. 4 (April 1994): 253e—253. http://dx.doi.org/10.21273/hortsci.29.4.253e.
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Our purpose was to study the impact of controlled atmosphere (CA) on respiration of and changes in sugar content of diced onions. The onions were peeled, diced, washed, disinfected, and centrifuged before storage for 12 days under gas mixtures of 21% O2 and 0% CO2 (air), 2% O2 and 0% CO2, and 2% O2 and 10% CO2. Every 4 days, respiration rate and sugar content (total, sucrose, fructose, and glucose) were determined. Carbon dioxide-enriched atmosphere limited respiration rate and sucrose depletion, whereas the mere reduction of O2 had no effect. Reducing sugar contents remained constant during storage regardless of the treatment.
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Jones, RB, JK Truett, and M. Hill. "Postharvest handling of cut immature Eucalyptus foliage." Australian Journal of Experimental Agriculture 33, no. 5 (1993): 663. http://dx.doi.org/10.1071/ea9930663.
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Optimum postharvest and storage treatments were investigated for cut immature branches of Eucalyptus crenulata Blakely & Beuzev. and Eucalyptus gunnii J. D. Hook. The application of the germicide BCDMH at 10 mg/L of active chlorine, 100 �L Agral-600/L. or 0.25% sucrose to vase solutions significantly enhanced vase life in E. crenulata Longevity in E. gunnii was significantly extended by sucrose (0.25-2% w/v), but not by germicides or Agral. Sucrose pulses (1-10% for 24 11 at 20�C) or exogenous ethylene (50 �L/L for 24 h at 20�C) had no effect on the longevity of either species. Both species produced very low levels of ethylene immediately after harvest and after a 24-h simulation of dry transport at 20�C. Longevity was not significantly altered in either species by 35 days of dry storage at 1�C. or by 7 days of wet storage (branches held in distilled water + 50 mg DICAJL) at 1�C, but declined significantly in both species after 7 days of wet storage at 10�C.
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Budiarto, Kurniawan, and Budi Marwoto. "Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture." Jurnal Natur Indonesia 13, no. 2 (November 21, 2012): 174. http://dx.doi.org/10.31258/jnat.13.2.174-177.
Full textAbstract:
Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage.
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Zhou, Shuqian, Lu Chen, Gang Chen, Yongxin Li, and Huqing Yang. "Molecular Mechanisms through Which Short-Term Cold Storage Improves the Nutritional Quality and Sensory Characteristics of Postharvest Sweet Potato Tuberous Roots: A Transcriptomic Study." Foods 10, no. 9 (September 2, 2021): 2079. http://dx.doi.org/10.3390/foods10092079.
Full textAbstract:
Sweet potato (Ipomoea batatas (L.) Lam.) is a commercially relevant food crop with high demand worldwide. This species belongs to the Convolvulaceae family and is native to tropical and subtropical regions. Storage temperature and time can adversely affect tuberous roots’ quality and nutritional profile. Therefore, this study evaluates the effect of storage parameters using physicochemical and transcriptome analyses. Freshly harvested tuberous roots (Xingxiang) were stored at 13 °C (control) or 5 °C (cold storage, CS) for 21 d. The results from chilling injury (CI) evaluation demonstrated that there was no significant difference in appearance, internal color, weight, and relative conductivity between tuberous roots stored at 13 and 5 °C for 14 d and indicated that short-term CS for 14 d promoted the accumulation of sucrose, chlorogenic acid, and amino acids with no CI symptoms development. This, in turn, improved sweetness, antioxidant capacity, and nutritional value of the tuberous roots. Transcriptome analyses revealed that several key genes associated with sucrose, chlorogenic acid, and amino acid biosynthesis were upregulated during short-term CS, including sucrose synthase, sucrose phosphate synthase, phenylalanine ammonia-lyase, 4-coumarate-CoA ligase, hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase, serine hydroxymethyltransferase, alanine aminotransferase, arogenate dehydrogenase, and prephenate dehydratase. These results indicated that storage at 5 °C for 14 d could improve the nutritional quality and palatability of sweet potato tuberous roots without compromising their freshness.
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Bích, Lê Như, and Nguyễn Thị Hồng Nhung. "SUCROSE PULSING AND COLD STORAGE ON POST-STORAGE ATTRIBUTES OF CUT LILY FLOWERS IN DALAT, VIETNAM." Tạp chí Khoa học Đại học Đà Lạt 10, no. 2 (May 4, 2020): 14. http://dx.doi.org/10.37569/dalatuniversity.10.2.575(2020).
Full textAbstract:
Ảnh hưởng của việc tiền xử lý đường và bảo quản lên tuổi thọ và đặc điểm của hoa lily cắt cành ở thành phố Đà Lạt được nghiên cứu. Sau khi thu hoạch, hoa lily được xử lý bằng dung dịch đường sucrose ở ba nồng độ (0, 50, và 100g/L) kết hợp với 0.2mM STS và xử lý với nước (đối chứng) trong 24h ở nhiệt độ phòng (23oC), sau đó bảo quản lạnh ướt và lạnh ở nhiệt độ 2.5oC. Sau một và hai tuần bảo quản lạnh, hoa lily được cắm trong nước để theo dõi thời gian cắm, thời gian bắt đầu nở hoa, tỷ lệ nở, và sự hấp thụ nước. Kết quả cho thấy khi hoa lily được tiền xử lý với dung dịch sucrose 50g/L, STS 0.2mM và bảo quản lạnh trong một tuần thì có thời gian cắm tăng (16.8 ngày) với màu sắc nở đẹp hơn, thời gian nở chậm hơn (5.8 ngày), các nụ hoa nở hoàn toàn (100%), và duy trì sự hấp thụ nước (91.5ml).
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Feller, Carmen, and Matthias Fink. "Refraction as a Measure of Soluble Carbohydrates in Storage Roots of Asparagus." HortScience 42, no. 1 (February 2007): 57–60. http://dx.doi.org/10.21273/hortsci.42.1.57.
Full textAbstract:
The objective of our study was to determine how accurately refractometry can quantify soluble carbohydrates in the storage roots of asparagus (Asparagus officinalis L.). Fructose, glucose, sucrose, and fructans as well as refraction were measured in 51 root samples that were taken from commercial fields. There was substantial variation in refraction both within roots of the same plant (cv, 6%) and within plants in the same field (cv, 20%). Samples of asparagus root sap contained fructose, glucose, sucrose, and fructans in varying fractions and, in addition, significant amounts of other solubles, which contributed considerably to refraction. Therefore, refraction readings are no direct measure of fructose, glucose, sucrose, and fructans in asparagus root sap. However, the concentration of these carbohydrates can be well estimated by a regression function, which uses refraction readings as input (r = 0.89).