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1

Gibberd, M. R., and P. S. Cocks. "Effect of waterlogging and soil pH on the micro-distribution of naturalised annual legumes." Australian Journal of Agricultural Research 48, no. 2 (1997): 223. http://dx.doi.org/10.1071/a96074.

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Variation in the size and composition of the soil seed bank of 3 naturalised legumes, subterranean clover (Trifolium subterraneum L.), woolly clover (T. tomentosum L.), and cluster clover (T. glomeratum L.), was related to soil characteristics on a transect that ascended from a winter waterlogged area in a wheatbelt pasture. Growth of the 3 species was compared with other species from section Vesicaria (T. resupinatum and T. clusii) in waterlogged and freely drained pots for 34 days. Of the 3 naturalised legumes present in the transect, cluster clover was rare, and woolly and subterranean clovers were separated on the basis of their response to soil pH and the likelihood of winter waterlogging. Subterranean clover was absent from soil with pH > 7·0; these areas were dominated by woolly clover. Areas with pH < 7·0 were dominated by subterranean clover, except where winter waterlogging was likely, in which case they were once again dominated by woolly clover. The waterlogging tolerance of woolly clover, together with that of other species in section Vesicaria, was confirmed in the pot experiment. After 34 days, shoot dry weight of the waterlogged Vesicaria species was an average of 39% greater than the freely drained controls. Root length continued to increase for the duration of the waterlogging treatment with much of the new root growth as laterals. Conversely, shoot and root growth in subterranean and cluster clovers was severely reduced by waterlogging. The patchy distribution of woolly clover in many wheatbelt pastures can be explained by its response to high pH and winter waterlogging compared with subterranean clover.
2

Norman, Hayley C., David G. Masters, Allan J. Rintoul, Matt G. Wilmot, Vijay Jayasena, Angelo Loi, and Clinton K. Revell. "The relative feeding value of a new pasture legume, eastern star clover (Trifolium dasyurum), compared with subterranean clover (Trifolium subterraneum)." Australian Journal of Agricultural Research 56, no. 6 (2005): 637. http://dx.doi.org/10.1071/ar04271.

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An accession of eastern star clover (Trifolium dasyurum C. Presl.), a new species to agriculture, has been identified by the National Annual Pasture Legume Improvement Program for commercial release in Australia. This paper reports the findings of an experiment designed to assess the feeding value of eastern star clover compared with a commercial cultivar of subterranean clover (T. subterraneum L. cv. Dalkeith). The hypothesis tested in this experiment is that sheep grazing eastern star clover will have similar growth rates, condition scores, and wool production to sheep grazing subterranean clover. Additionally, it is hypothesised that the sheep grazing the different clover species will have similar health and meat quality. The relative feeding value of the 2 clover species was tested using 14-month-old Merino wethers, grazing 8 by 0.5 ha plots (4 of each species). Stocking rates were such that herbage mass availability would not have limited feed intake. Liveweight, wool growth, and animal health were monitored. After 10 weeks of grazing, 2 animals from each plot were slaughtered for sensory analysis of meat. Although there were generally no differences in liveweight change, condition, or wool growth between sheep grazing eastern star or subterranean clovers, sheep in the subterranean clover plots grew faster during the first month of grazing. There were no differences in meat eating quality (tenderness, juiciness, or flavour), animal health, or vegetable matter contamination of wool. The data suggest that the clovers had the same relative feeding value despite herbage mass from the eastern star clover plots having lower in vitro digestibility, crude protein, and higher fibre than herbage mass from the subterranean clover plots. Given that there was a minimum of 1000 kg/ha of herbage mass in the plots, it is likely that sheep gazing the two clovers were able to maintain the same weight by selecting the high quality components of the pasture. Differences in plant quality are associated with differences in reproductive strategies of the clovers.
3

Conning, S. A., M. Renton, M. H. Ryan, and P. G. H. Nichols. "Biserrula and subterranean clover can co-exist during the vegetative phase but are out-competed by capeweed." Crop and Pasture Science 62, no. 3 (2011): 236. http://dx.doi.org/10.1071/cp10218.

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Biserrula (Biserrula pelecinus L.) is a recently domesticated annual pasture legume developed for ley farming systems that have traditionally relied upon subterranean clover (Trifolium subterraneum L.). This study examined competitive interactions between biserrula and subterranean clover and the common broad-leaf weed capeweed (Arctotheca calendula L.) during seedling establishment and vegetative growth, in order to develop guidelines for successful legume pasture management. Two glasshouse experiments were conducted to investigate the allocation of biomass to roots and shoots in biserrula, capeweed, and subterranean clover and its relationship with competitive ability in the first 100 days after sowing. In Experiment 1, capeweed had a higher relative growth rate of shoots and roots than the two legumes and developed a more extensive root system. Experiment 2 consisted of growing binary mixtures of the three species at different densities. The effect of competition on the biomass of biserrula, capeweed, and subterranean clover was best modelled by a power–exponential model. Increasing capeweed densities suppressed the biomass production of both biserrula and subterranean clover, whereas capeweed biomass increased with increasing densities of subterranean clover. This study suggests that the competitive advantage of capeweed is mainly conferred during the seedling stage. It also suggests that biserrula and subterranean clover germinating at the same time can co-exist as a mixed sward, at least up until flowering, if biserrula density is high relative to subterranean clover.
4

Chapman, R., T. J. Ridsdill-Smith, and N. C. Turner. "Water stress and redlegged earth mites affect the early growth of seedlings in a subterranean clover/capeweed pasture community." Australian Journal of Agricultural Research 51, no. 3 (2000): 361. http://dx.doi.org/10.1071/ar99108.

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The impact of water stress and infestations of redlegged earth mite [Halotydeus destructor Tucker (Acarina : Penthaleidae)] on the early growth and botanical composition of a mixed subterranean clover (Trifolium subterraneum L.) and capeweed (Arctotheca calendula Levyns) pasture was investigated in a controlled environment experiment. Water stress and redlegged earth mite infestations both significantly reduced herbage production from both species. The yield of the subterranean clover was suppressed less by water stress than that of the capeweed. The differing sensitivities of the two species to water stress were attributed to differences in seedling size and growth rates at the onset of the drought. Redlegged earth mites caused greater feeding damage on cotyledons of the subterranean clover than of the capeweed. Despite this, the mites had a greater deleterious impact on the growth of the capeweed, which was suppressed both in the presence and absence of water stress. Redlegged earth mites in the presence of water stress did not significantly affect the growth of the subterranean clover. Furthermore, in the absence of water stress, the growth of the subterranean clover was greater when mites were present than when absent. The greater sensitivity of the capeweed to the effects of feeding by the redlegged earth mites was attributed to the smaller size of its seedlings at the time the redlegged earth mites were introduced. The increase in growth of the subterranean clover following the introduction of redlegged earth mites is more likely due to a change in the competitive relationships between the two plant species than to any direct effect of the mites’ feeding. Our observations indicate that the presence of water stress and redlegged earth mites significantly affects the competitive interactions between seedlings of subterranean clover and capeweed.
5

Eden, T. M., P. J. Gerard, D. J. Wilson, and N. L. Bell. "Effects of invertebrate pests on white and annual clovers in dryland soil." New Zealand Plant Protection 63 (August 1, 2010): 235–40. http://dx.doi.org/10.30843/nzpp.2010.63.6574.

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Two experiments were carried out at Ruakura in soil taken from Whatawhata hill country pasture The relative susceptibility of several annual clovers (Trifolium spp) and perennial white clover (T repens) to slugs (Deroceras reticulatum) clover root weevil (Sitona lepidus) adults native crickets (Nemobius sp) or wheat bugs (Nysius huttoni) was tested by sowing seed of the clovers into separate rows in turf Susceptibility of clovers to clover cyst nematode (Heterodera trifolii) was tested by sowing each clover variety into Whatawhata soil inoculated with the nematode Plant growth was assessed in both experiments 4 weeks after sowing White clover was more susceptible to pests than the annual clovers with slugs and clover root weevil significantly reducing seedling survival and clover cyst nematode significantly reducing plant growth Subterranean clover (T subterraneum) cv Denmark was the least affected by pests showing no significant reduction in survival in the presence of slugs the most damaging pest and no significant decrease in plant root and shoot dry weight when exposed to nematodes
6

Rogers, ME, and CL Noble. "The effect of NaCl on the establishment and growth of balansa clover (Trifolium michelianum Savi var. balansae Boiss.)." Australian Journal of Agricultural Research 42, no. 5 (1991): 847. http://dx.doi.org/10.1071/ar9910847.

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The effect of NaCl on the germination, seedling emergence and growth of established plants of balansa clover, Trifolium michelianum Savi var. balansae Bois. cv. Paradana was studied and compared with plants of subterranean clover T: subterraneum L. cv. Clare. Balansa and subterranean clover had similar salt tolerances at germination and seedling emergence. However balansa clover suffered from poor seedling vigour even under non-saline conditions, and this resulted in a low number of emerged seedlings over all NaCl concentrations compared with subterranean clover. Established plants of balansa clover, despite a lower initial seedling yield, were significantly more salt tolerant (P < 0.001) and had a greater survival rate over a four month period at concentrations greater than 75 mol m-3 NaCl. Shoot concentrations of chloride and sodium increased with increasing salinity in both species, however concentrations were lower in balansa clover especially at higher NaCl concentrations (75 and 100 mol m-3 NaCl). It is concluded that whilst established stands of balansa clover may be able to withstand high concentrations of NaCl, the major limitation to its establishment and production in saline irrigation areas is its poor seedling emergence and vigour.
7

Smetham, M. L. "Subterranean clover (Trifolium subterraneum): its history and current and future research in New Zealand." NZGA: Research and Practice Series 11 (January 1, 2003): 61–72. http://dx.doi.org/10.33584/rps.11.2003.2994.

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This review covers the history of subterranean clover (Trifolium subterraneum) and its present occurrence in New Zealand; a review of research and conclusions to be drawn from this; and examines past and future research directions. Subterranean clover has been important in terms of area of use in New Zealand since the late 1930's. Today it makes a significant contribution to pasture production on steep, north-facing North Island hill country where effective rainfall is low, and in other areas where soil moisture drops below wilting point for between 2 weeks and 5 months of the year. Research has identified the superiority of cultivars possessing a compact, ground-hugging habit of growth, and flowering late, in late October - early November, for use in North Island hill country. In drier areas, more research is needed to identify superior cultivars, although there is evidence that lines in the mid-season or early mid-season flowering groups may be appropriate. A major problem for the use of subterranean clovers in New Zealand is the slow breakdown of hardseededness. About 7 M ha of land in New Zealand is mapped as being subject to slight to severe moisture deficit (an area which could with advantage support subterranean clover), but further research is needed to identify appropriate cultivars for 4.8 M ha or more of this area. Key words: history, New Zealand, research, Trifolium subterraneum
8

Coventry, DR, JR Hirth, and KKH Fung. "Nutritional restraints on subterranean clover grown on acid soils used for crop-pasture rotation." Australian Journal of Agricultural Research 38, no. 1 (1987): 163. http://dx.doi.org/10.1071/ar9870163.

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Lime, N and molybdenum fertilizers were used to establish how soil acidity limits the growth of subterranean clover (Trifolium subterraneum), in pot culture, on acid soils taken from north-east Victoria. The results indicate that two nutritional factors associated with the growth of subterranean clover are affected by excess acidity. These are manganese toxicity and ineffective nodulation of the clover. The plants grown on one unlimed soil (Rutherglen) had manganese concentrations and symptoms consistent with manganese toxicity. Liming the second soil (Lilliput) overcame a N deficiency by way of increased nodule formation. A third nutritional factor commonly associated with excess acidity, and found on the Lilliput soil, molybdenum deficiency, was not alleviated by the application of lime. Instead, the application of molybdenum fertilizer gave increases in clover dry matter production at all lime rates. In the absence of molybdenum fertilizer, the molybdenum concentration of the herbage was extremely low, irrespective of liming. These results are discussed in relation to already established field responses of subterranean clover to lime, and the management of the clover-ley system on the strongly acid soils.
9

Norman, Hayley C., Angelo Loi, Matt G. Wilmot, Allan J. Rintoul, Bradley J. Nutt, and Clinton K. Revell. "Sheep grazing bladder clover (Trifolium spumosum L.) had similar productivity and meat quality to sheep grazing subterranean clover (Trifolium subterraneum L.)." Animal Production Science 53, no. 3 (2013): 209. http://dx.doi.org/10.1071/an12185.

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An accession of bladder clover, a new species to agriculture, was selected for commercial release in Australia with the cultivar name of AGWEST Bartolo. As part of a duty-of-care assessment, we tested the hypothesis that sheep grazing the bladder clover cultivar will have similar liveweight, condition scores and wool production to sheep grazing a widely adopted subterranean clover cultivar (cv. Dalkeith). Further, we hypothesised that sheep grazing bladder clover and subterranean clover will have similar meat quality (after 48 days of grazing) and health as indicated by muscle, liver and kidney panel analysis. The data supported the hypotheses. While there were significant initial differences in liveweight between the two groups of sheep, there were no significant differences in liveweight at later time points during the grazing period and no significant differences in mean daily growth rate between weighing times. There were no differences in wool growth between sheep grazing the bladder or subterranean clover cultivars. Sheep in the subterranean clover plots had significantly higher condition scores after the first 24 days of grazing; however, these differences were not apparent after another 24 days of grazing. There were some differences in indications of animal health; however, there were no significant detrimental effects associated with bladder clover and sheep grazing both species had mean plasma indicators that were deemed ‘healthy’. There were no differences in meat eating quality as estimated by a consumer panel for tenderness, juiciness or flavour. Dry seasonal conditions limited biomass growth and the sheep were removed from the subterranean clover plots on Day 48 of grazing. The sheep grazing the bladder clover plots remained on the plots for a further 26 days. The data indicate that, for the conditions of this experiment, the clovers had the same relative feeding value. Laboratory analysis of herbage from across the clover plots (bulked before analysis) suggest that the bladder clover had higher in vitro digestibility, crude protein and lower fibre than the herbage sample from the subterranean clover plots. The biomass data indicate that the bladder clover cultivar can support more grazing days per ha in a relatively dry season than the subterranean clover cultivar.
10

Nichols, P. G. H., M. J. Barbetti, P. M. Evans, A. D. Craig, G. A. Sandral, B. S. Dear, P. Si, and M. P. You. "Napier subterranean clover (Trifolium subterraneum L. var. yanninicum)." Australian Journal of Experimental Agriculture 46, no. 8 (2006): 1109. http://dx.doi.org/10.1071/ea05084.

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Napier is a late flowering F6-derived crossbred subterranean clover of var. yanninicum [(Katz. et Morley) Zohary and Heller] developed by the collaborating organisations of the National Annual Pasture Legume Improvement Program. It is a replacement for both Larisa and Meteora and has been selected for release on the basis of its greater herbage and seed production and disease resistance to both known races of clover scorch and 2 of the common races of Phytophthora root rot. Napier is recommended for sowing in Victoria, Western Australia, New South Wales, and South Australia. It is best suited to moderately acidic soils prone to water-logging and to loamy and clay soils with good water-holding capacity in areas with a minimum growing season length of 7.5 months, which extends into late November. Napier is well adapted to the permanent pasture systems found in the areas in which it will be grown. Its upright, vigorous growth makes it well suited to grazing by cattle or sheep and to fodder conservation. Napier has been granted Plant Breeders Rights in Australia.
11

Evans, P. M., S. Walton, P. A. Riffkin, and G. A. Kearney. "Effect of plant density on the winter production of annual clovers grown in monocultures." Australian Journal of Experimental Agriculture 42, no. 2 (2002): 135. http://dx.doi.org/10.1071/ea01003.

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The small-seeded annual clovers, balansa and Persian, are often assumed to be poor winter producers. Their small seed size, of about 1 mg or less, and poor regeneration, possibly due to inappropriate grazing management in many instances, contributes to this perception. To test the hypothesis that early growth of these clovers is determined by the weight of germinating seed, as it is in subterranean clover, an experiment with 2 cultivars of subterranean clover, Leura and Trikkala, 2 cultivars of balansa clover, Paradana and Bolta, and 1 of Persian clover, Nitro Plus, was established in the field at Hamilton, western Victoria, at 6 sowing densities. The winter production at 2 additional sites, Lake Bolac and Streatham, in their third and second and third seasons, respectively, was also examined. Plant density varied from 30 to 37000 plants/m2 across sites and species. With equal weight of germinating seed per unit area at sowing, balansa and Persian clovers produced more herbage in winter than did the 2 subterranean clover cultivars Leura and Trikkala (P<0.05). Even though there was high correlation between seed weight and seedling weight across all species shortly after emergence (r2 = 0.99), by harvest time no differences in plant weight existed between any treatments growing at the same plant density. From this we conclude the following: (i) for the same weight of germinable seed per unit area, balansa and Persian clovers produced more dry weight per hectare than subterranean clover, because they had higher plant densities; (ii) there were no differences in dry matter production per hectare between species growing at similar plant densities by harvest time at the end of winter; (iii) it appeared that in winter the small-seeded species exhibited a higher relative growth rate than the 2 subterranean clovers.
12

Edmeades, DC, FPC Blamey, CJ Asher, and DG Edwards. "Effects of pH and aluminium on the growth of temperate pasture species. II. Growth and nodulation of legumes." Australian Journal of Agricultural Research 42, no. 5 (1991): 893. http://dx.doi.org/10.1071/ar9910893.

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Ten temperate pasture legumes inoculated with appropriate rhizobia were grown for 31 days in flowing solution culture. Solution ionic strength was approximately 2700 8M and contained inorganic nitrogen (150 , 8M NO3-) only at the commencement of the experiment. Solution pH was maintained at 4.5, 5.0, 5.5 and 6.0. Also, five aluminium (Al) treatments were imposed, with nominal Al concentrations of 0, 3, 6, 12 and 24 8M (2.5, 7.1, 8.3, 11.2 and 24.7 8M Al measured) at pH 4.5. Solution pH <6 . 0 markedly reduced total dry mass (TDM) in all cultivars of white clover (Trifolium repens) cvv. 'Grasslands Pitau, Huia, G18 and Tahora' and red clover (Trifolium pratense) cvv. 'Grassland Turoa and Pawera', and to a lesser extent in the two subterranean clover (Trifolium subterraneum) cvv. 'Tallarook and Woogenellup'. In contrast, solution pH had no effect on the growth of Lotus corniculatus cv. Maitland, while Lotus pedunculatus cv. Maku grew best at pH 4.5. Lotus pedunculatus cv. Maku grew best in solution where the sum of the activities of the monomeric Al species {Alm} was maintained at 5.9 8M. The growth of all other species was decreased with Al in solution, a 50% reduction in TDM being associated with c. 6 8M {Alm] for white clover and subterranean clover, and c. 3 8M in red clover and Lotus corniculatus cv. Maitland.
13

Leys, A., and B. Plater. "Simazine mixtures for control of annual grasses in pastures." Australian Journal of Experimental Agriculture 33, no. 3 (1993): 319. http://dx.doi.org/10.1071/ea9930319.

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The effectiveness of tank mixes of simazine plus paraquat, and simazine plus fluazifop-P, for control of annual grass weeds in subterranean clover (Trifolium subterraneum L.) pastures was compared in 6 field experiments in southern New South Wales between 1989 and 1991. Mixtures of simazine (500-750 g a.i./ha) plus very low rates of paraquat (40-60 g a.i./ha) gave better than 90% control of vulpia [Vulpia bromoides (L.) S. F. Gray and V. myuros (L.) C. C. Gmelin], barley grass (Hordeum leporinum Link), annual ryegrass (Lolium rigidum Gaud.), and great brome (Bromus diandrus Roth) and were at least as effective as mixtures of simazine plus fluazifop-P (53-106 g a.i./ha). Subterranean clover plant density was not affected by any treatment. Excellent control of grasses caused increased subterranean clover dry matter yields at 1 site and no differences at another site. In weed-free conditions, 12 of 21 herbicide treatments damaged subterranean clover (severe leaf burn and retarded growth) and significantly (P<0.05) reduced dry weight, but this was not reflected in subsequent seed yields.
14

Muir, S. K., A. J. Kennedy, G. Kearney, P. Hutton, A. N. Thompson, P. Vercoe, and J. Hill. "Offering subterranean clover can reduce methane emissions compared with perennial ryegrass pastures during late spring and summer in sheep." Animal Production Science 60, no. 11 (2020): 1449. http://dx.doi.org/10.1071/an18624.

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Sheep production systems in south-west Victoria are based predominantly on perennial ryegrass pastures, resulting in highly seasonal growth and declining feed quantity and nutritive value in late spring and summer. These changes result in reduced animal performance and increased CH4 emissions per kg DM intake. A potential alternative to the feedbase used in south-west Victoria that provides high quality and quantity of feed in late spring and early summer are legume-based pastures, such as clovers and lucerne. This experiment examined the impact of legume-based pastures on the growth rates and CH4 emissions of Maternal Composite ewes during late spring and early summer. In 2014, 240 Maternal Composite ewes grazed either perennial ryegrass (Lolium perenne L.), lucerne (Medicago sativa L.), subterranean clover (Trifolium subterraneum L.) or arrowleaf clover (Trifolium vesiculosum Savi.) pastures for 6 weeks during late spring and early summer (November and December). Sheep grazing subterranean clover were heavier at the end of the experiment than sheep grazing perennial ryegrass. Methane measurements using portable accumulation chambers indicated lower daily CH4 emissions (g/day) from sheep grazing subterranean clover (23.5 g/day) than from sheep grazing lucerne (27.3 g/day) and perennial ryegrass (32.3 g/day) pastures. Methane emissions and liveweight changes appeared to be associated with the nutritive characteristics of the forage offered. Legume-based pastures provide sheep producers in south-west Victoria an option to increase growth rates and decrease CH4 emissions during a period when perennial ryegrass pastures are declining in nutritive value.
15

Hill, MJ. "Sward growth of monocultures and binary mixtures of phalaris, lucerne, white clover and subterranean clover under two defoliation regimes." Australian Journal of Experimental Agriculture 31, no. 1 (1991): 51. http://dx.doi.org/10.1071/ea9910051.

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Sirosa phalaris, WL5 15 lucerne, Haifa white clover and Seaton Park subterranean clover were grown in monocultures and binary mixtures on a cracking clay soil and cut at 4- and 8-week intervals over 3 growing seasons at Scone, New South Wales (32�S.). The plots were given supplementary irrigation between mid April and November to prevent water deficits. The deep-rooted perennials dominated mixtures under infrequent cutting, with cumulative dry matter yields for the growing season reaching 11 t/ha for lucerne-based mixtures. Frequent defoliation increased the contribution of the clovers in mixtures, and mixtures and monocultures containing clovers were more productive than other plots in winter, 1986. Frequent defoliation reduced the vigour and density of lucerne, resulting in balanced mixtures with phalaris and subterranean clover in the third year. Only lucerne plots contributed large amounts of dry matter yield (up to 4 t/ha) between December and April. Regeneration of subterranean clover from seedlings, and white clover from seedlings or stolons, was better in clover-only plots than in mixtures with phalaris. The growth of mixtures in response to mean daily air temperature in spring could be divided into 2 distinct patterns, with growth peaking at about 15�C for determinate (annual or dormant) mixtures, and at about 18�C for lucerne mixtures and pure white clover. In autumn, growth patterns were determined by the method of regeneration (i.e. by seedings or dormant crowns).
16

Horsnell, LJ. "The growth of improved pastures on acid soils. 2. The effect of soil incorporation of lime and phosphorus on the growth of subterranean clover and lucerne pastures and on their response to topdressing." Australian Journal of Experimental Agriculture 25, no. 1 (1985): 157. http://dx.doi.org/10.1071/ea9850157.

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Subterranean clover responds poorly to superphosphate application on some acid soils of the Southern Tablelands of New South Wales. A field experiment was undertaken, for two years, to examine the effects of incorporating large additional amounts of superphosphate or rock phosphate in the soil, with and without lime, on the growth of subterranean clover, lucerne and phalaris sown with recommended rates of lime superphosphate. Dry matter responses of subterranean clover and lucerne to superphosphate topdressing in the second year were also recorded. In the first year, subterranean clover growth was increased by the additional lime and by lime plus superphosphate. Lucerne growth was increased by additional lime. In the second year, the growth of subterranean clover was increased by the lime treatments and the superphosphate treatments applied in the previous year and by the deep incorporation into the soil of lime and superphosphate together. Subterranean clover growth also responded to the application of rock phosphate without lime. Lucerne dry matter production in the second year was increased by the lime, superphosphate and rock phosphate treatments applied in the first year. Lime application increased the yield responses of subterranean clover and lucerne to superphosphate topdressed in the second year. Lime application had no effect on the nitrogen content of the clover but increased that of lucerne. Lime application reduced the aluminium levels in the tops of all three species. The data suggest that the responsiveness of pastures to superphosphate on these soils is increased by the application of lime and rock phosphate and is related to low nitrogen fixation and high aluminium levels in the plant.
17

Tang, C., L. Barton, and C. Raphael. "Pasture legume species differ in their capacity to acidify soil." Australian Journal of Agricultural Research 49, no. 1 (1998): 53. http://dx.doi.org/10.1071/a97080.

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The capacity of subterranean clover (Trifolium subterraneum L. cv. Clare), medic (Medicago murex Willd. cv. Zodiac), serradella (Ornithopus sativus Brot. line SP1/13), biserrula (Biserrula pelecinus L. line Mor99), and woolly clover (Trifolium tomentosum L.) to acidify soil under N2 fixation was compared in a pot experiment using a poorly buffered sandy soil. The amount of acid produced per kg shoot dry matter (specific acid production) varied betweefin species and with growth stages, ranging from 44 to 128 cmol/kg shoot. Subterranean clover and serradella acidied soil to a greater extent than woolly clover and medic, whereas biserrula acidified soil least. Irrespective of pasture species and growth stage, specific acid production correlated well with concentrations of excess cations and calcium in shoots. Furthermore, total excess cation, ash alkalinity, and calcium in shoots were all good indicators of total acid production across all of the species.
18

Stirzaker, RJ, BG Sutton, and N. Collis-George. "Soil management for irrigated vegetable production. I. The growth of processing tomatoes following soil preparation by cultivation, zero-tillage and an in situ-grown mulch." Australian Journal of Agricultural Research 44, no. 4 (1993): 817. http://dx.doi.org/10.1071/ar9930817.

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A sequence of lettuce and tomato crops was grown in a field experiment on a sandy loam soil in order to study the relationship between soil tillage and plant growth under the near optimal water and nutrient conditions which are characteristic of vegetable production. This paper describes the performance of processing tomato crops, grown over two seasons under a range of soil management systems. The soil management systems included a cultivated and a zero-tillage treatment, as well as a zero-tillage treatment which followed a crop of subterranean clover (Trifoliurn subterraneum L.). The subterranean clover was sown in autumn and senesced in early summer to form an in-situ mulch. The bulk density of the soil in the zero-tillage treatment was high, and this was associated with a large reduction in vegetative growth, compared with the cultivated treatment. The bulk density of the soil was also high in the treatment where the subterranean clover was grown, but the vegetative growth of tomatoes was equal to that in the cultivated treatment. Despite the large differences in vegetative growth among treatments, there was no significant difference in the fruit yield. These results show that processing tomatoes can be successfully grown under zero-tillage when irrigation and fertilizer are well managed, and that vegetative growth and fruit yield are poorly correlated. The in-situ mulch of subterranean clover improved the physical conditions of the soil compared to the zero-tillage treatment, successfully controlled weeds and reduced soil erosion, but at the cost of having land out of production during the winter.
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Banik, Bidhyut Kumar, Zoey Durmic, William Erskine, and Clinton Revell. "Anti-methanogenic advantage of biserrula (Biserrula pelecinus) over subterranean clover (Trifolium subterraneum) from in vitro fermentation is maintained across growth stages and cutting treatments." Crop and Pasture Science 70, no. 3 (2019): 263. http://dx.doi.org/10.1071/cp18069.

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The annual forage legume biserrula (Biserrula pelecinus L.) offers a promising opportunity as a low-methanogenic, bioactive pasture for southern Australian grazing systems where subterranean clover (Trifolium subterraneum L.) is the dominant annual pasture legume. This in vitro study to assess methanogenic potential examined how growth stage and cutting of biserrula affect biomass, nutritive value and fermentative parameters including methanogenic potential compared with subterranean clover. Both species were grown in a glasshouse, where three growth-stage treatments comprised plant collection at vegetative, reproductive or maturity growth stages. Three cutting (simulated grazing) treatments included cutting at the vegetative or reproductive stage and an uncut control, with herbage collection at maturity. Methane production in biserrula was unaffected by the stage of growth. Other nutritive value and fermentative parameters varied significantly with growth stage, the highest fibre content and lowest crude protein (CP) being found at maturity. Regrowth from herbage cut at the reproductive stage had higher CP and lower biomass than other cut treatments. In biserrula, this regrowth also showed the highest fermentability of the three cutting treatments including elevated methane yield. Notwithstanding these treatment effects on fermentability, biserrula maintained its strong anti-methanogenic advantage over subterranean clover across all treatments, confirming its potential as an anti-methanogenic bioactive pasture.
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Costello, Tom and Anne. "Subterranean clover in North Canterbury sheep pastures." NZGA: Research and Practice Series 11 (January 1, 2003): 189–92. http://dx.doi.org/10.33584/rps.11.2003.3011.

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The Hawarden basin is typified by dry, hot conditions over summer, which makes it difficult to retain ryegrass/white clover pastures on the lighter soils. These conditions have led to the introduction of better-adapted species such as the annual, subterranean clover. The mid-flowering cultivar 'Mt Barker' was first sown in the 1930s; then the earlyflowering 'Woogenellup' and late 'Tallarook' were used in the 1960s. The 'Mt Barker' type became dominant in these old pastures. The key factors for good subterranean clover growth were soil fertility (particularly P and S), space following dry summers for seedlings to re-establish, and moisture in the spring to maximise herbage yield. A visit to Australia in 1996 prompted the inclusion in new sowings of the late-flowering 'Leura' at high sowing rates (8-10 kg/ ha). It has taken 5 years for seed stocks to build to a level where we can identify good seedling strikes of 'Leura'. Two of the autumns over this period were very difficult and hindered normal establishment. Rainfall is erratic in North Canterbury, which affects the success of subterranean clover from one year to another. Dry summer conditions are optimal for subterranean clover strikes, as the pasture opens, and when the autumn break occurs, more than 2 000 subterranean clover seedlings/m2 can be produced. Autumn grazing management, when recovering from extreme drought, is critical because pasture must be spelled for 6 weeks to allow the subterranean clover to establish. However, if the autumn is wet, pasture cover must be controlled so that subterranean clover seedlings are not smothered. Set-stocking is best in spring to control pasture cover, but grazing should not be too hard during the flowering and seed set period. Subterranean clover seed yields of up to 250 kg/ha have been achieved from old pastures, but this is less seed than is produced under Australian conditions. Pasture renovation using the baker boot drill to re-introduce grasses and new cultivars of subterranean clover has been successful following very dry summers. Lamb growth rates have been excellent on subterranean clover-based pastures and these have increased the farm's meat production and reduced the cost of maintaining pastures. Key words: grazing management, North Canterbury, seedling establishment, seed set, subterranean clover
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O'Rourke, Tiernan A., Megan H. Ryan, Tim T. Scanlon, Krishnapillai Sivasithamparam, and Martin J. Barbetti. "Amelioration of root disease of subterranean clover (Trifolium subterraneum) by mineral nutrients." Crop and Pasture Science 63, no. 7 (2012): 672. http://dx.doi.org/10.1071/cp12239.

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Subterranean clover (Trifolium subterraneum) is a key pasture legume across southern Australia and elsewhere. Decline in subterranean clover pastures was first recognised in Australia during the 1960s and manifests as an increase in weeds and a decrease in desirable legume species. While both root disease and poor nutrition contribute to subterranean clover pasture decline, the relationships between root disease and nutrition have not been determined. The objective of this study was to define these relationships. Field experiments were undertaken to determine the nutritional and pathogen status of soils and subterranean clover from three Western Australian field sites. Subsequently, controlled environment experiments were undertaken to determine the relative severities of tap and lateral root disease and growth of plants when soil cores taken from these three field sites were amended with a complete nutrient solution or a range of individual macro- or micronutrient treatments. Application of a ‘Hoaglands’ complete nutrient solution decreased the severity of tap root disease by an average of 45% and lateral root disease by 32%. Amendment with K alone reduced the severity of tap root disease an average of 32%; while the application of N alone reduced the severity of tap root disease by 33% and lateral root disease by 27%. Application of Hoaglands, K, N or Zn increased shoot and root dry weight, while Mo only increased shoot dry weight. This is the first report to show that mineral nutrients can substantially ameliorate root disease in subterranean clover. The results demonstrate that while root disease limits plant growth, improvement in the nutritional status of nutrient-impoverished soils can significantly reduce root disease. There is significant potential to incorporate nutrient amendments into an integrated and more sustainable approach to better manage root disease and to increase productivity of pasture legumes where soils are inherently nutrient deficient in one or more nutrients.
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Pinkerton, A., and PJ Randall. "Sulfur requirements during early growth of Trifolium balansae, Trifolium subterraneum, Medicago murex, and Phalaris aquatica." Australian Journal of Experimental Agriculture 35, no. 2 (1995): 199. http://dx.doi.org/10.1071/ea9950199.

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Sulfur (S) requirements for early growth were determined for 3 legumes (Trifolium balansae, T. subterraneum, Medicago murex) and 1 grass (Phalaris aquatica). Plants were grown in sand culture in a glasshouse and supplied with nutrient solutions containing 6 rates of S (1-32 �g/mL). Legumes were supplied with nitrogen (N) at 168 �g/mL, and phalaris with 28 or 168 pg N/mL. Plants were sampled twice, the second sampling coinciding with flowering of the legumes. Diagnostic indices [total S (St), HI-reducible S (sulfate-S), oxidised S (S6+)], and ratios (sulfate-S/St, S6+/St) were derived for whole shoots (WS), youngest open leaves (YOL) of legumes, and youngest expanded blades (YEB) of the grass. The largest responses to S by the legumes were made by balansa clover and murex medic, which both outyielded subterranean clover, but subterranean clover was the most efficient user of S and had the lowest external S requirement. Concentrations of S6+ were always higher than concentrations of sulfate-S, but the 2 were highly correlated and much lower than St. No S fraction or plant part sampled was consistently superior as an indicator of S status. The larger relative increases and sharper breaks in gradient of the relationships between yield and sulfate-S or S6+ compared with St were offset by their greater relative variability. All indices were subject to Piper-Steenbjerg effects, although these did not seriously affect the critical values. Critical values of St and S6+ in YOL, St in WS of balansa clover, and S, and S6+ in WS of subterranean clover changed little up to 60 days after sowing, but it was necessary to know plant age when assessing the S status of murex medic or phalaris. Critical concentrations of S, in phalaris were little affected by N supply, but critical concentrations of sulfate-S and S6+ were higher with 28 than 168 �g N/mL. Critical values for subterranean clover agreed well with previously published data.
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Hyslop, M. G., M. W. A. Slay, and C. A. Moffat. "Dry matter accumulation and sheep grazing preference of six winter active annual legumes." NZGA: Research and Practice Series 11 (January 1, 2003): 117–21. http://dx.doi.org/10.33584/rps.11.2003.3006.

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Winter active legumes are suited to the mild winter areas of New Zealand, where they provide high quality forage in late winter and early to mid-spring. Six winter active annual legumes, persian clover, balansa clover, sweet clover, hybrid serradella, subterranean clover, and Medicago truncatula, were sown in a randomised complete block design with four replicates. Their dry matter (DM) accumulation and grazing preference to young sheep were measured every 3-6 weeks from sowing on 6 April 2000 until 24 October 2000. Total ungrazed DM production ranged from 5 500 kg/ha (sweet clover) to 9 300 kg/ha (balansa clover) over this period. The seasonal pattern of growth varied significantly between species, as did hogget grazing preference, with persian, balansa, and subterranean clover most preferred. These six plant species are capable of producing large amounts of high quality dry matter, but at all grazings, preference was extreme (measured by the number of sheep preferring to graze any one of the species when given a choice of all six), which may indicate the limited usefulness of some species. Key words: accumulative yield, grazing preference, Medicago truncatula, Melilotus albus, Ornithopus sativus x compressus, seasonal growth, sheep, Trifolium balansae, Trifolium respinatum, Trifolium subterraneum, winter active legumes
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Alva, AK, CJ Asher, and DG Edwards. "The role of calcium in alleviating aluminium toxicity." Australian Journal of Agricultural Research 37, no. 4 (1986): 375. http://dx.doi.org/10.1071/ar9860375.

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Effects of varying calcium (Ca) concentration at constant or varying solution ionic strength on root elongation of soybean (Glycine max (L.) Merr.) and subterranean clover (Trifolium subterraneum L.) were determined in aluminium (Al) free nutrient solution or solutions containing monomeric Al activities (+aAl mono) of 8-10 8M for subterranean clover and 20-22 8M for soybean. In Al-free solutions, the root length for subterranean clover was not significantly influenced by Ca or ionic strength. However, soybean root length was greater at 500 8M Ca than at higher Ca concentrations. Raising the ionic strength at 500 and 5000 8M Ca significantly decreased root length. In the presence of Al, maximum root length of both species occurred at 15 000 8M Ca. Soybean root length at 500 and 5000 8M Ca was 35% and 87% respectively of that at 15000 8M Ca. The corresponding values for subterranean clover were 53% and 81%. The positive effect of Ca concentration on root length, despite a nearly constant +aAL mono, confirms the existence of a protective action of Ca against Al toxicity. Raising the solution ionic strength at 500 8M Ca in the presence of Al improved the root growth of soybean by 86% and that of subterranean clover by 45%. At 5000 8M Ca, a small beneficial effect of increased ionic strength (14%) was found only in subterranean clover. Increasing Ca concentration in solution decreased water extractable and 0-1 M HNO3 extractable Al in roots of both plant species. Transfer of soybean seedlings to Al-free nutrient solutions containing 500, 1500 or 5000 pM Ca after 24, 48 or 96 h in a solution containing +aAL mono of 22 8M resulted in a substantial recovery in primary root growth. Relative root lengths were in each case significantly higher at 5000 8M Ca than at 1500 or 500 pM Ca. Roots transferred to 500 8M Ca after exposure to Al for 6 or 18 h underwent a period of accelerated elongation after a lag period of 30-40 h. By 138 h there were no significant differences in root length between the unstressed control plants and those subjected to 6 or 18 h Al-stress.
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Dear, B. S., and P. S. Cocks. "Effect of perennial pasture species on surface soil moisture and early growth and survival of subterranean clover (Trifolium subterraneum L.) seedlings." Australian Journal of Agricultural Research 48, no. 5 (1997): 683. http://dx.doi.org/10.1071/a96121.

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Subterranean clover seedling numbers and growth in swards containing 1 of 5 perennial pasture species [phalaris (Phalaris aquatica) cv. Sirolan, cocksfoot (Dactylis glomerata) cv. Currie, lucerne (Medicago sativa) cv. Aquarius, wallaby grass (Danthonia richardsonii) cv. Taranna, and lovegrass (Eragrostis curvula) cv. Consol] were compared with those in typical annual pastures and pure clover swards in the wheatbelt of eastern Australia. Presence of a perennial species or the volunteer annual grass (Eragrostis cilianensis) increased the rate of drying of the soil surface (0–5 cm) after late February and May rain, compared with subterranean clover swards. Perennials differed in the rate they dried the soil surface, with the more summer-active lucerne and consul lovegrass drying the profile more rapidly than phalaris. The amount of water in the surface 5 cm, 6 days after the rainfall event on 27–28 February, was strongly negatively correlated (r = –0·75, P < 0·01) with the amount of green perennial biomass, but not related to standing dead material or surface residues. Where perennials were present, a smaller proportion (2–4%) of the clover seed pool produced seedlings in response to late summer rain, compared with pure clover swards (18%). A higher proportion of the seed pool produced seedlings (19–36%) following rain in late autumn but there was no difference between species. The more summer-active perennials (cocksfoot, danthonia, and lucerne) markedly depressed the survival of emerged clover seedlings following both germinations. Of the seedlings that emerged in early March, the proportion remaining by 29 March was 57% in phalaris, 21% in lucerne, 13% in danthonia, and 1% in cocksfoot, compared with a 78% increase in seedlings in pure subterranean clover swards. By 15 May, all perennials had <2 clover seedlings/m2 surviving, compared with 37 in the annual pasture and 964 plants/m2 in pure subterranean clover. Following the May germination, the highest proportion of emerged seedlings surviving until 29 May was in the phalaris swards (40%) and least in the cocksfoot and danthonia swards (2–4%). Presence of a perennial or annual grass decreased (P < 0·05) relative water content of clover seedlings on 15 March from 74% in pure clover swards, to 48% in annual pasture, 34% in phalaris, and 29% in lucerne swards. Clover seedlings growing in pure subterranean swards on 15 March (17 days after germinating rain) were 4 times larger than those in lucerne and twice as large as those in either phalaris or annual pasture. Seed size did not differ between treatments, but available mineral soil nitrogen was significantly higher (P < 0·001) in pure subterranean clover swards (32 mg N/g) compared with perennials (3–13 mg N/g). Strategies such as heavy grazing in late summer to reduce green biomass of the perennials or sowing the perennials at lower densities may reduce the adverse effects that perennials have on subterranean clover seedlings in these drier environments.
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Humphries, A. W., S. S. Robinson, D. Hawkey, D. M. Peck, T. D. Rowe, C. T. de Koning, and A. Newman. "Diversity for resistance to a moderately virulent bluegreen aphid (Acyrthosiphon kondoi Shinji) population in Trifolium species." Crop and Pasture Science 67, no. 9 (2016): 1009. http://dx.doi.org/10.1071/cp15102.

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The resistance of a diverse range of Trifolium species (clovers) to a highly virulent bluegreen aphid (BGA, Acyrthosiphon kondoi Shinji) population (Urrbrae 2011) collected in South Australia was assessed in greenhouse and field experiments, with the aims of determining the potential impact of this insect pest on biomass and identifying resistant genotypes for future plant-breeding activities. Resistance to BGA was found in populations of clovers that show some level of outcrossing—white clover (T. repens L.), rose clover (T. hirtum All.), crimson clover (T. incarnatum L.) and red clover (T. pratense L.)—and in one entry of the inbreeding subspecies of subterranean clover, T. subterraneum L. subsp. subterraneum (Katzn. and Morley). Resistance was not found in T. s. brachycalycinum (Katzn. and Morley) or T. s. yanninicum (Katzn. and Morley). In a greenhouse experiment, damage from BGA resulted in forage yield penalties of 72–100% when aphids were inoculated at 14 days after sowing and 13−74% when inoculated at 42 days after sowing, showing that in optimum conditions BGA can be a serious pest of clovers. Observations of severe damage caused by BGA in two regenerating field trials in southern New South Wales confirmed that field damage could occur in seasons favourable to aphid growth and reproduction. The severe damage that BGA can cause to clovers, and the sources of resistance we found, suggest that breeding for BGA resistance in clovers is warranted and feasible.
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Lewis, T. R., R. J. Lucas, R. W. Hofmann, and D. J. Moot. "Tolerance of newly sown cocksfoot-clover pastures to the herbicide imazethapyr." Journal of New Zealand Grasslands 79 (January 1, 2017): 173–80. http://dx.doi.org/10.33584/jnzg.2017.79.552.

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In New Zealand, subterranean clover is recommended as a companion legume in mixed swards, particularly in dryland cocksfoot-based pastures. However, establishment of cocksfoot is slower than perennial ryegrass and therefore weed ingress is more common. An experiment at Lincoln University, Canterbury showed imazethapyr applied when clover was at the 3-4 trifoliate leaf stage, and cocksfoot at the 2+ leaf stage, increased the subterranean clover content of the pastures by at least 1000 kg DM/ha, despite initial visual phytotoxicity responses. Balansa and white clover pasture yields were not different to their unsprayed unweeded controls. Imazethapyr application controlled broadleaf weeds from early in the season. The herbicide application reduced cocksfoot yields by 70% in early spring, but yields recovered and were not different to the unsprayed unweeded controls at 1350 ± 260 kg DM/ha after grazing. Imazethapyr application improved subterranean clover pastures through an increase in clover content by suppressing weeds and temporarily reducing the rate of cocksfoot growth. Keywords: subterranean clover, Spinnaker herbicide, seedling, white clover, balansa clover
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Dear, BS, and J. Lipsett. "The effect of boron supply on the growth and seed production of subterranean clover (Trifolium subterraneum L.)." Australian Journal of Agricultural Research 38, no. 3 (1987): 537. http://dx.doi.org/10.1071/ar9870537.

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There has been little research on the boron (B) status of subterranean clover, despite strong indications of deficiency of B in southern Australia where clover decline has been reported. This paper describes glasshouse experiments to vary the level of B supply to clover grown on soils low in B. On a soil farmed in a cereal clover rotation, herbage yields of clover increased by 25% with applied B, but seed yields, negligible without B, increased 21-fold. On two other acid soils, from pastures, there were also large responses to B in seed yield (1.5- and 5.1-fold respectively); liming increased the responses (8- and 55.2-fold). However, herbage production was less responsive to B (25% and 1.7-fold with liming); on the unlimed soils, application of B depressed yield (- 1 and -21%). This is attributed to there being enough B to sustain herbage growth, but inadequate for seed formation. These results show that herbage yield, conventionally employed in the past, is not a satisfactory index of B status for total performance in subterranean clover. The response to B in seed yield was associated with increases in: numbers of seed set (75% to 22.6-fold, depending on the particular combination of soil and liming); size of seed (10% to 1.2-fold); proportion of buried seed (20%-70%). It is suggested that these favour establishment and persistence of clover and that clover decline may well involve deficiency of B. It was shown that concentration of B in the plant does not necessarily relate predictably to yield of herbage and that reduction in seed yield may not be heralded by foliar symptoms, since seed requirement exceeds that of herbage.
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Thompson, A. N., A. J. Kennedy, J. Holmes, and G. Kearney. "Arrowleaf clover improves lamb growth rates in late spring and early summer compared with subterranean clover pastures in south-west Victoria." Animal Production Science 50, no. 8 (2010): 807. http://dx.doi.org/10.1071/an09100.

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The profitable production of lamb from pasture-based systems is dependent on high levels of pasture production being efficiently utilised by sheep of superior genetic merit. Pastures that can extend the pasture-growing season and provide high quality feed in late spring and summer have the potential to increase production efficiency and the proportion of lambs that meet market specifications. In this paper we tested the hypothesis that arrowleaf clover (cv. Arrotas), a cultivar selected for late maturity, would supply feed of higher nutritive value than conventional annual legumes and perennial ryegrass mixtures during December and January and this would improve lamb growth rates over this period. Two experiments were conducted to compare the nutritive value of pasture and growth rates of lambs grazing arrowleaf clover, subterranean clover (cv. Leura) or subterranean clover/perennial ryegrass mixtures at different stocking rates during late spring and early summer in south-west Victoria. In the second experiment an arrowleaf clover/perennial ryegrass treatment was also included. Lambs were removed from the plots when they could no longer maintain liveweight. Arrowleaf clover extended the growing season by 4–6 weeks and provided feed of significantly higher digestibility than subterranean clover during December and January. The digestibility of arrowleaf and perennial ryegrass was comparable, but arrowleaf had significantly higher crude protein content during this period. Lambs grazing arrowleaf clover at stocking rates up to 24 lambs/ha grew at or above 100 g/day until the end of January, whereas lambs grazing subterranean clover and perennial ryegrass mixed pastures could not maintain weight by mid-to-late December in both years and were removed from the pastures. At the highest stocking rates total lamb production from arrowleaf clover monoculture pastures in late spring and early summer was ~400 kg liveweight/ha, and was more than double that achieved from the subterranean clover and perennial ryegrass/subterranean clover mixed pastures. The perennial ryegrass/arrowleaf clover mixed pastures in the second experiment produced a similar quantity of lamb per hectare as the arrowleaf clover monoculture pastures at the same stocking rate. In this paper we demonstrated that late-maturing arrowleaf clover can be a profitable, special purpose lamb-finishing pasture. Arrowleaf clover was most suited to more elevated parts of the landscape where estimated carrying capacity was 20 to 40% greater than that from lower lying parts of the landscape that were more subject to water logging and weed infestation. The risks of including arrowleaf clover pastures into the farming system could be reduced by direct drilling arrowleaf clover, which has relatively poor winter production, into existing perennial ryegrass pastures.
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Aldaoud, R., W. Guppy, L. Callinan, S. F. Flett, K. A. Wratten, G. A. Murray, T. Cook, and A. McAllister. "Occurrence of Phytophthora clandestina in Trifolium subterraneum paddocks in Australia." Australian Journal of Experimental Agriculture 41, no. 2 (2001): 187. http://dx.doi.org/10.1071/ea00048.

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In 1995–96, a survey of soil samples from subterranean clover (Trifolium subterraneum L.) paddocks was conducted across Victoria, South Australia, New South Wales and Western Australia, to determine the distribution and the prevalence of races of Phytophthora clandestina (as determined by the development of root rot on differential cultivars), and the association of its occurrence with paddock variables. In all states, there was a weak but significant association between P. clandestina detected in soil samples and subsequent root rot susceptibility of differential cultivars grown in these soil samples. Phytophthora clandestina was found in 38% of the sampled sites, with a significantly lower prevalence in South Australia (27%). There were significant positive associations between P. clandestina detection and increased soil salinity (Western Australia), early growth stages of subterranean clover (Victoria), mature subterranean clover (South Australia), recently sown subterranean clover (South Australia), paddocks with higher subterranean clover content (Victoria), where herbicides were not applied (South Australia), irrigation (New South Wales and Victoria), cattle grazing (South Australia and Victoria), early sampling dates (Victoria and New South Wales), sampling shortly after the autumn break or first irrigation (Victoria), shorter soil storage time (Victoria) and farmer’s perception of root rot being present (Victoria and New South Wales). Only 29% of P. clandestina isolates could be classified under the 5 known races. Some of the unknown races were virulent on cv. Seaton Park LF (most resistant) and others were avirulent on cv. Woogenellup (most susceptible). Race 1 was significantly less prevalent in South Australia than Victoria and race 0 was significantly less prevalent in New South Wales than in South Australia and Western Australia. This study revealed extremely wide variation in the virulence of P. clandestina. The potential importance of the results on programs to breed for resistance to root rot are discussed. in South Australia.
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Lattimore, MAE, HG Beecher, and KL O'Callaghan. "Establishment and early growth after rice of subterranean clover (Trifolium subterraneum), Persian clover (T. resupinatum), balansa clover (T. michelianum), and white clover (T. repens)." Australian Journal of Experimental Agriculture 34, no. 4 (1994): 459. http://dx.doi.org/10.1071/ea9940459.

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Four temperate clovers were grown on a typical rice-growing soil in the Murrumbidgee Valley, New South Wales, to assess their establishment and early growth potential. Subterranean clover (Trifolium subterraneum var. yanninicum) cv. Trikkala, Persian clover (T. resupinatum) cv. Maral, balansa clover (T. michelianum var. balansae) cv. Paradana, and white clover (T. repens) cv. Haifa were sown immediately following a rice crop in autumn of 3 consecutive years. Seven sowing methods were compared, including combinations of surface or drill sowing with land preparation techniques commonly used in rice-farming systems. All cultivars established successfully. Trikkala had the best establishment on the basis of per cent seed sown in year 2, and equal best in years 1 and 3, although Paradana and Maral had higher plant densities at the sowing rates used. Paradana and Maral produced the highest dry matter yields and Haifa the lowest (P<0.05) in the growing period to mid-spring. Sowing method strongly influenced (P<0.05) both establishment and yield of clover. Sowing into a dense rice canopy before harvest severely restricted clover performance, while harvesting rice before sowing greatly improved (P<0.05) clover establishment and dry matter production. Establishment from surface sowing was generally more successful than from drill sowing, although yields with both methods were mostly satisfactory. Establishment and yields were highest after burning stubble, but this effect was variable and not always significant. Sowing after cultivation was not successful, especially in the 2 wetter years, due to poor seedbed preparation and waterlogging.
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Bolland, MDA. "Effect of soil acidity and nutrient deficiencies on the growth and persistence of subterranean clover in pastures grown on sandy soils near Esperance, Western Australia." Australian Journal of Experimental Agriculture 25, no. 4 (1985): 893. http://dx.doi.org/10.1071/ea9850893.

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Most of the sandy soils near Esperance, W.A., were cleared of native vegetation and sown to subterranean clover in the 1950s and 1960s. Over the past 20-30 years, pH values of the topsoil (1:5, soil: water) have decreased from 6.5-7.0 to 5.0-5.5. The application of 2 t/ha of agricultural lime increased the soil pH (1:5, 0.01 CaCl2) from 5.5 to 5.8, from 4.9 to 5.6, and from 5.1 to 5.5 at three sites that had been sown to subterranean clover for 10, 20 and 40 years respectively. However, lime had no effect on either dry matter production or seed yield of subterranean clover. Additions of calcium in a further experiment also did not affect the seed yield of subterranean clover. Irrespective of the lime treatments, the addition of nutrients (phosphorus, potassium, sulfur, copper, zinc, molybdenum, cobalt, manganese and boron) significantly (P<0.05) improved dry herbage yields by a factor of 1.3, 1.7 and 1.4 at the 10-, 20- and 40-year-old sites. However, the addition of nutrients did not affect seed yields of subterranean clover. In other experiments, the omission of sulfur, potassium and, for the deepest sandy site, phosphorus reduced dry herbage yields by between 10-30%. The omission of molybdenum from treatments of the 20- and 40-year-old pasture reduced herbage yields by 21 and 16% respectively. However, omission of molybdenum in the experiment sited on the 10-year-old pasture did not affect yields. Thus, as the acidification of the sandplain soils continues, molybdenum may become deficient and may limit herbage yields of subterranean clover.
33

Zhang, X., P. M. Evans, and P. A. Riffkin. "Performance of annual pasture legumes in cropping rotations in the cool temperate zone of south-eastern Australia." Australian Journal of Experimental Agriculture 44, no. 9 (2004): 863. http://dx.doi.org/10.1071/ea03093.

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The relative performance of 3 alternative pasture legumes, in comparison with subterranean clover, grown in a 1:1 pasture:crop rotation was investigated during 1997–2001, at 2 sites in the high rainfall (>500 mm/year) zone of southern Australia. Seedling densities of the pasture legumes (before and after crop) were examined, along with herbage dry matter production, growth pattern and seed production. After 2 cycles of a 1:1 pasture:crop rotation, average seedling densities of balansa (Trifolium michelianum Savi var. michelianum), Persian (T. resupinatum L. var. resupinatum Gib. and Belli), arrowleaf (T. vesiculosum Savi.) and subterranean (T. subterraneum L.) clovers at the 2 sites were 6810, 2910, 680 and 630 plants/m2, respectively. Average cumulative herbage mass in 2001 was 7.49, 5.21, 8.53 and 7.76 t DM/ha.year, respectively. All clovers produced a large seed pool: balansa, 71.8 g/m2; subterranean, 38.9 g/m2; Persian, 35.5 g/m2; and arrowleaf, 35.4 g/m2. The results suggest that a 1:1 pasture:crop rotation is sustainable, in terms of pasture seed–seedling dynamics for each of the legumes, and that under this system the pasture legumes need to be sown only once.
34

Mulholland, JG, KS Nandra, GB Scott, AW Jones, and NE Coombes. "Nutritive value of subterranean clover in a temperate environment." Australian Journal of Experimental Agriculture 36, no. 7 (1996): 803. http://dx.doi.org/10.1071/ea9960803.

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A study was made over 2 years of the nutritive value of the subterranean clover (Trifolium subterraneum L.) cultivars, Trikkala, Larisa and Goulburn, in grazing experiments with Border Leicester x Merino lambs. Liveweight gain was similar for all cultivars in the first year and in the second year was higher on Larisa (153 g/day) compared with Goulburn and Trikkala (131 and 103 g/day, respectively). Wool growth was not significantly different between cultivars. Both the digestibility and water-soluble carbohydrate content of the petiole and stem plant fraction were significantly (P<0.05) higher than those of the leaf fraction, even though the leaf had a lower level of cell wall organic matter. The ratio of lignin to cell wall organic matter was also higher in the leaf fraction. Samples obtained via oesophageal fistula showed that petioles comprised most of the dry matter in the diet and, thus, were the major contributor to energy supply. Differences were found in the degradability characteristics of the 3 cultivars which led to significant (P<0.05) differences in the concentration of rumen degradable dry matter and effective rumen degradable protein (ERDP). During the vegetative and early flowering stages, the concentration of ERDP exceeded the supply of fermentable metabolisable energy required for microbial protein synthesis. In mature clover, the concentration of ERDP was low and limiting microbial protein synthesis. The degradation characteristics of protein were highly correlated with liveweight gain (r2 = 0.90) and wool growth (r2 = 0.70). It is suggested that increasing the petiole : leaf ratio could increase the efficiency of utilisation of subterranean clover diets.
35

Thomson, C. J., C. K. Revell, N. C. Turner, M. A. Ewing, and I. F. Le Coultre. "Influence of rotation and time of germinating rains on the productivity and composition of annual pastures in Western Australia." Australian Journal of Agricultural Research 49, no. 2 (1998): 225. http://dx.doi.org/10.1071/a94082.

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A long-term rotation experiment located in south-western Australia was used to measure the effect of rotation and time of germinating rains on the productivity and botanical composition of grazed annual pastures in 2 contrasting seasons in an environment with an average annual rainfall of 325 mm. The density of self-regenerating seedlings of subterranean clover (Trifolium subterraneum), capeweed (Arctotheca calendula), and grasses (Lolium rigidum, Hordeum leporinum, Bromus diandrus) was greatly increased (approx. 3 times the density) when there was a second year of pasture after crop compared with the first year after crop. The lower plant density resulted in first-year pastures having only about 33% of the autumn biomass accumulation of second-year pastures. This difference in early pasture growth had no effect on total pasture production in 1992, but in 1993 total pasture production was 30% greater in second-year pastures compared with first-year pastures. Botanical composition varied between and within seasons with the percentage of subterranean clover increasing throughout the season and the percentage of capeweed decreasing throughout the season. Grasses comprised <20% of the biomass in all seasons and treatments. Production of subterranean clover seed in 1993 was higher in a 1 : 2 crop-pasture rotation than in a 1 : 1 crop-pasture rotation and direct drilling in the cropping phase increased seed set compared with conventional tillage in both 1 : 1 and 1 : 2 crop-pasture rotations. Capeweed seedlings emerged in large numbers after rainfall between February and May and subsequently showed a relative growth rate twice that of subterranean clover and the grasses, but exclusion of rainfall until June resulted in a significant reduction in the emergence of capeweed seedlings. Additionally, capeweed had a lower rate of seedling survival compared with other pasture species, and this is contrary to observations by other researchers that capeweed is highly resistant to moisture stress during early growth.
36

Rossiter, RC. "Effects of early defoliation on the seedling growth of subterranean clover." Australian Journal of Agricultural Research 43, no. 3 (1992): 717. http://dx.doi.org/10.1071/ar9920717.

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Two experiments were conducted in an open-sided glass shelter at Perth during winter. Subterranean clover (Trifolium subterraneum, L.) seedlings were grown in shallow seed boxes at low plant densities, 2 4 and 2 0 plants/dm2. The effects of a single defoliation involving mainly the removal of cotyledons only, on shoot growth and leaf production were measured at harvest, c. 5 weeks after sowing. Total shoot dry weights were reduced by 15-30% when 40-60% of the leaf area was removed by single defoliations from days 7 to 19 after planting. However, the growth was reduced by almost 70% when both cotyledons were removed (days 7 to 9) before emergence of the unifoliate leaf, i.e. when all of the leaf area was removed. In general, the relative reduction in growth largely depended on the percentage of leaf area removed, rather than on time of removal. Defoliation also reduced the size of trifoliate leaves, and total numbers of trifoliate leaves at harvest. There was a linear relationship between total leaf numbers and shoot dry weights. In the field, these findings are probably of more relevance in terms of insect damage, especially from redlegged earth mites, than for grazing by sheep.
37

Davey, A. G., A. P. Henderson, and R. J. Simpson. "Nodulation and growth of subterranean clover (Trifolium subterraneum L.) in a drying acid soil." Soil Biology and Biochemistry 21, no. 1 (January 1989): 1–8. http://dx.doi.org/10.1016/0038-0717(89)90002-3.

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38

Wolfe, E. C., J. A. Paul, and P. D. Cregan. "Monitoring ley pastures and their response to winter cleaning." Australian Journal of Experimental Agriculture 46, no. 8 (2006): 1023. http://dx.doi.org/10.1071/ea04074.

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The purposes of this study were to evaluate subterranean clover-based leys on farms and in experiments using several pasture parameters, and to assess the impact of winter cleaning on the productivity and botanical composition of clover swards. Annual pastures were monitored on a group of 5 farms in the Wagga district and compared with an experimental subterranean clover (Trifolium subterraneum L.) pasture. The major problem in the farm paddocks was a lack of legume biomass due to poor legume densities, a consequence of the use of the soft-seeded cultivar Woogenellup and a high content of grassy weeds. The farmers in the group were unaware of the tools, parameters and benchmarks for making quantitative pasture comparisons. In 2 experiments, a range of subterranean clover swards were generated through the use of cultivars, seeding rate and winter cleaning treatments, grazed at 15 sheep/ha and monitored for 3 years. Appropriate benchmark values for the seed pool of subterranean clover were 300–350 kg/ha in winter and 600–700 kg/ha in summer. On the basis of both winter production, a function of May seedling density (target >1000 seedlings/m2) and spring production, which depended on the cultivar maturity, Junee was superior at Wagga to either Dalkeith (earlier maturing) or Woogenellup (softer seeded). Winter cleaning, using selective herbicides (fluazifop, simazine) to remove grasses and weeds, was advantageous in achieving a high content (>90%) and productivity of subterranean clover, provided that the legume content of the pasture was at least 28%, or >20% of total ground area before herbicide application in winter. In winter-cleaned swards, legume growth increased by up to 80%, legume biomass was improved by up to 46% and legume content increased from <50 to >95%. The main disadvantages of winter cleaning were increased areas of bare ground and reduced total biomass for several weeks after herbicide application, and the rapid development of ryegrass that was resistant to at least 1 of the herbicides used. The strategic use of observations to monitor the performance of pastures and their response to management is discussed.
39

Tozer, K. N., R. J. Lucas, and G. R. Edwards. "Suppression of annual grass weeds by AR542 endophyte infection in dryland tall fescue pastures." New Zealand Plant Protection 60 (August 1, 2007): 164–67. http://dx.doi.org/10.30843/nzpp.2007.60.4676.

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The effect of endophyte infection of tall fescue (with and without AR542 endophyte Neotyphodium coenophialum) plant competition (white versus Caucasian clover with and without subterranean clover overdrilled) and spring stocking rate (10 versus 20 ewes/ha) on the population size of annual grass weeds was monitored in a pasture on dry stony soils in Canterbury Barley grass (Critesion murinum) cover and seedhead production were lower in AR542 than endophyte free pastures and where subterranean clover was overdrilled than where it was not Vulpia hair grass (Vulpia spp) seedhead production and cover were lower where subterranean clover was overdrilled and where Caucasian rather than white clover was sown Ewe stocking rate did not affect annual grass weed cover or seedhead density The results indicate that sowing tall fescue with AR542 endophyte may be a useful management tool to enhance growth of the perennial grass and reduce invasion of barley grass into dryland pastures
40

Scott, BJ, and AD Robson. "Distribution of magnesium in subterranean clover (Trifolium subterraneum L.) in relation to supply." Australian Journal of Agricultural Research 41, no. 3 (1990): 499. http://dx.doi.org/10.1071/ar9900499.

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The objectives of this study were to examine Mg distribution in subterranean clover (Trifolium subterraneum L.), to identify an appropriate tissue for diagnosis of deficiency and to establish minimum tissue concentrations associated with maximum plant growth. Plants were grown in solution culture with both discontinued (40 8M and 160 8M reduced to nil) and constant supply (0, 5, 10, 20, 40, 80, 160 8M Mg). Magnesium was depleted from old leaves when Mg supply to the roots was interrupted. However, deficiency symptoms occurred first on the young tissue under these conditions. Under constant but inadequate supply, initial deficiency symptoms occurred in old tissue. Symptoms in leaves were associated with Mg concentrations in the leaf of < 1046 8g g-1 with constant root supply and 586 8g g-1 when supply was interrupted. At luxury constant supply, concentrations of Mg tended to be higher in the older leaves than in the young leaves; the reverse occurred with inadequate supply. Tissue choice for diagnosis was not critical, but the minimum Mg concentration in tissue commensurate with maximum shoot growth varied from 740 8g g-1 for the unifoliate leaf to 1310 8g g-1 in the youngest open leaf (YOL), when plants were grown under constant Mg supply. Current supply of Mg to roots was reflected more rapidly in the concentrations in young leaf than in old leaf tissue.
41

Rossiter, RC, WJ Collins, and L. Klein. "Winter growth and nutritive quality of serradella (Ornithopus spp.)." Australian Journal of Experimental Agriculture 25, no. 2 (1985): 362. http://dx.doi.org/10.1071/ea9850362.

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Two genotypes of yellow serradella (Ornithopus compressus) and one of French serradella (O. sativus) were compared with subterranean clover when grown as swards in boxes in an open-sided glass shelter at Perth, Western Australia. The swards were defoliated at 1.5 cm, at weekly intervals, from 4 weeks after sowing until harvest at almost 15 weeks. The total yield of herbage was as high in serradella as in subterranean clover, although net herbage production (i.e. plant material removed by defoliation) was 9% less in the serradella because the sward was shorter during early growth. After 10 weeks, net herbage production rates were similar for the four legumes (5.4-5.8 g/m2.day). No major differences between the legumes were found in several measures of nutritional quality.
42

Peoples, M. B., R. R. Gault, G. J. Scammell, B. S. Dear, J. Virgona, G. A. Sandral, J. Pau, E. C. Wolfe, and J. F. Angus. "Effect of pasture management on the contributions of fixed N to the N economy of ley-farming systems." Australian Journal of Agricultural Research 49, no. 3 (1998): 459. http://dx.doi.org/10.1071/a97014.

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The effects of different management regimes on N2 fixation by subterranean clover (Trifolium subterraneum) in annual pastures and lucerne (Medicago sativa) in perennial-based pastures were examined in 5 experiments and 55 commercial paddocks, in which the pastures were grown in phased rotation with crops. The objectives were to quantify the inputs of fixed N2 and to determine ways of increasing nitrogen (N) inputs into ley-farming systems of southern New South Wales and north-eastern Victoria. Estimates of annual amounts of N2 fixed, based on above-ground herbage production in grazed pastures, ranged from 5 to 238 kg N/ha for subterranean clover and from 47 to 167 kg N/ha for lucerne. Legume reliance upon N2 fixation for growth (Pfix) was high (>65%) in most annual and perennial pastures examined. The levels of Pfix were generally unaffected by management treatments. As a consequence the amounts of N2 fixed were predominantly regulated by the legume content and herbage yield of pastures rather than by any marked differences in the ability of the legume to fix N. When all experimental results were combined with on-farm measurements of N2 fixation, the data indicated that lucerne and subterranean clover fixed 22-25 kg N for every tonne of legume dry matter produced. Management inputs to annual pastures which improved the productivity of subterranean clover and the amounts of N2 fixed included applications of superphosphate and the removal of grass species with herbicide, although the response to these treatments was not consistent across all sites in all years. Potential inputs from N2 fixation were high in annual pastures, and improved management during a good clover season enhanced the levels of mineral N detected in the soil profile (0-200 cm) the following autumn by 100-200 kg N/ha. However, year-to-year variability in annual pasture productivity and clover content resulted in large fluctuations in amounts of N2 fixed. Perennial pastures containing lucerne provided consistently greater annual herbage production, had more stable legume contents, and fixed on average 90-150% more N2 than neighbouring subterranean clover-based pastures. Even during the 1994 drought when annual pastures failed, lucerne still managed to fix >70 kg N/ha. It is proposed that lucerne-based pastures could represent a more reliable means of improving soil fertility for subsequent crops than annual pastures.
43

Hochman, Z., GJ Osborne, PA Taylor, and B. Cullis. "Factors contributing to reduced productivity of subterranean clover (Trifolium subterraneum L.) pastures on acidic soils." Australian Journal of Agricultural Research 41, no. 4 (1990): 669. http://dx.doi.org/10.1071/ar9900669.

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In a field study on four sites, soil acidity, root rot (Phytophthora clandestina), and soil phosphorus were identified as causes of 'subterranean clover decline'. Liming increased herbage and seed production at four sites, with a tendency for lime to increase herbage yields in autumn (22%) and winter (15%) but not in spring. The presence of ryegrass with clover increased total herbage yields, and reduced clover seed production, but there was no interaction with liming. Losses caused by root rot associated with P. clandestina were quantified for the first time in New South Wales. Root rot reduced survival of seedlings as well as herbage production in autumn and/or winter at three of the four sites. In the presence of the disease, lime did not improve root health or seedling survival. On two sites with high aluminium saturation of exchangeable cations (> 17%) and high phosphorus sorption index values, subterranean clover growth responded to high levels of P fertilizer. On one site, where lime increased the soil pH to above 5.5, the P sorption index was temporarily increased, and this was associated with a temporary adverse effect on herbage yields. Some possible mechanisms underlying the seasonality of lime responses are proposed and the practical implications of our findings are discussed.
44

Paynter, BH. "Comparative phosphate requirements of yellow serradella (Ornithopus compressus) burr medic (Medicago polymorpha var. brevispina) and subterranean clover (Trifolium subterraneum)." Australian Journal of Experimental Agriculture 30, no. 4 (1990): 507. http://dx.doi.org/10.1071/ea9900507.

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The phosphate (P) requirements of burr medic (Medicago polymorpha) on marginally acidic, medium-textured soils and yellow serradella (Ornithopus compressus) on acidic, light-textured soils in the low rainfall (<400 mm) wheatbelt of Western Australia are not known. The hypothesis that yellow serradella, subterranean clover (Trifolium suhrerraneum) and burr medic have the same external requirement for applied P was tested in a glasshouse trial on 2 P-deficient soils. Species were compared on an equal total seed weight and similar maturity length basis. It was found that yellow serradella required less soil applied P to achieve 90% maximum shoot production or total plant (shoots + roots) growth and had a greater curvature co-efficient from fitted Mitscherlich functions than either subterranean clover or burr medic. Burr medic was the most responsive to applied P and had the largest external requirement for applied P. These differences in external P requirements were related to differences in ability of the 3 legumes to absorb P and to transport it to their shoots and were not due to differences in internal efficiency of the shoots. Yellow serradella was able to take up more P (total plant P content) per g roots than subterranean clover, and subterranean clover more than burr medic. The same relationship applied to the translocation of absorbed P from roots to shoots.
45

Masters, D. G., G. Mata, C. K. Revell, R. H. Davidson, H. C. Norman, B. J. Nutt, and V. Solah. "Effects of Prima gland clover (Trifolium glanduliferum Boiss cv. Prima) consumption on sheep production and meat quality." Australian Journal of Experimental Agriculture 46, no. 3 (2006): 291. http://dx.doi.org/10.1071/ea05036.

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Gland clover (Trifolium glanduliferum Boiss) is a recently introduced and released clover species for use in Mediterranean agricultural systems in southern Australia. There is little information on the expected animal production from this pasture plant although it is known to contain coumarins. Coumarins may have a direct effect on the production of grazing ruminants through a reduction in feed intake and may also have the ability to influence the flavour and odour of meat. In this study, Merino hogget ewes were grazed on eight 0.5 ha replicate plots sown to monocultures of either Prima gland clover or Dalkeith subterranean clover (Trifolium subterraneum ssp. subterraneum L.). The Prima gland clover was estimated to contain between 80 and 300 mg coumarin/kg dry matter. Three sheep were allocated to the plots on 7 September and 1 sheep from each plot was slaughtered on 29 September. Two additional sheep were allocated to the plots on 28 September and another 2 on 19 October. At the end of the experiment on 8 November, each plot contained 2 sheep that had grazed for 3, 6 or 9 weeks. All sheep were slaughtered at this time. The 2 sheep that remained on the plots from 7 September to 8 November were used for the comparison of animal production between the 2 clover species. Following slaughter, measurements were made of carcass characteristics and meat eating quality. The liveweight gain and wool growth of the 2 treatments were similar over the 9 weeks, however, the sheep on the Prima gland clover plots grew faster over the first 3 weeks. The faster growth rate is consistent with a higher in vitro digestibility of the Prima gland clover at this time. The similarity in feeding value of the 2 clover species indicates that there was no depression in feed intake associated with the Prima gland clover. There were some small significant differences in meat eating quality between the groups. Meat from sheep grazing the Prima gland clover was drier and had a more pleasant taste. These differences are unlikely to be large enough to have any commercial significance. The results indicate that the Prima gland clover has a feeding value at least equivalent to the Dalkeith subterranean clover and that, under the conditions of this experiment, the coumarin concentrations in the Prima gland clover are not high enough to affect either feed intake or the flavour and odour of meat.
46

Kim, MK, DG Edwards, and CJ Asher. "Tolerance of Trifolium subterraneum cultivars to low pH." Australian Journal of Agricultural Research 36, no. 4 (1985): 569. http://dx.doi.org/10.1071/ar9850569.

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Eleven cultivars of Trifolium subterraneum and Trifolium semipilosum cv. Safari were grown with adequate combined nitrogen for 27 days in flowing solution culture with controlled nutrient concentrations at constant pH values ranging from 3.5 to 6.5. A solution pH of 3.5 was lethal to all cultivars, but growth was in all cases vigorous at pH 4.0 (RGR 15.2-16.9 g 100 g-1 day-1). There were no significant effects of pH over the range of 4.0-6.5 on the yield of any clover cultivar. The results are discussed in relation to an earlier study suggesting greater tolerance of subterranean clover to pH values below 4.0. Phosphorus toxicity symptoms developed in all subterranean clover cultivars with the intensity of symptom development increasing with solution pH from 4.5 to 6.5. The concentration of phosphorus in the older leaves decreased as the solution pH was increased from 4.0 to 4.5, and then increased with further increase in pH, reaching values = 1.0%. Rates of phosphorus absorption followed a similar pattern of response to solution pH. Results are discussed with reference to previously reported effects of pH on phosphorus uptake.
47

Ziehm, R. W., H. A. Pearson, T. L. Thurow, and V. C. Baldwin. "Pine growth response to management of the subterranean clover understory." Agroforestry Systems 20, no. 3 (December 1992): 267–74. http://dx.doi.org/10.1007/bf00053144.

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48

Hall, DG, EC Wolfe, and BR Cullis. "Performance of breeding ewes on lucerne-subterranean clover pastures." Australian Journal of Experimental Agriculture 25, no. 4 (1985): 758. http://dx.doi.org/10.1071/ea9850758.

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Pasture production, ewe and lamb growth, ewe wool production and diet quality were studied on lucerne-subterranean clover pastures at Wagga Wagga, New South Wales. Lucerne was sown at rates of 0.75 to 3.0 kg/ha, and the pastures were rotationally grazed with Border Leicester x Merino ewes at 9.6 or 12.7 sheep/ha, the ewes lambing in August- September. Lucerne density declined by 45% over the 3 years on all treatments. The clover cultivar sown, Woogenellup, had low persistence, particularly at 12.7 sheep/ha. The density of lucerne had little effect on annual wool and lamb production, although the ewes grew faster on the denser lucerne in summer and the sparser lucerne in winter. At 12- 7 sheep/ha, there was an extra 19% total lamb weight by the end of November and an extra 22% of finer wool (1 �m) annually, but the fleeces had a higher proportion of wool tenderness. The major limitations of the lucerne-subterranean clover pastures to sheep production were the low quality of the diet in early summer, and low pasture production in late winter. In early summer the lucerne was rapidly consumed, leaving only moderate quality clover and grass residues, which limited lamb growth, while in winter pregnancy toxaemia occurred, fleeces were tender and wool growth was low, particularly during a drought in 1976.
49

Thomson, C. J., and T. P. Bolger. "Effects of seed phosphorus concentration on the emergence and growth of subterranean clover (Trifolium subterraneum)." Plant and Soil 155-156, no. 1 (October 1993): 285–88. http://dx.doi.org/10.1007/bf00025038.

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50

Edmeades, DC, FPC Blamey, CJ Asher, and DG Edwards. "Effects of pH and aluminium on the growth of temperate pasture species. I. Temperate grasses and legumes supplied with inorganic nitrogen." Australian Journal of Agricultural Research 42, no. 3 (1991): 559. http://dx.doi.org/10.1071/ar9910559.

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A flowing solution culture experiment was conducted in which four temperature pasture grasses and 11 temperate pasture legumes were grown in solutions of low ionic strength for 29 days at six aluminium (Al) levels with average Al3+ activities {Al3+} of 1.0, 5.1, 5.9, 9.9, 20.8, 41.6 8M at pH 4.5. Growth of these species was also measured at pH 5.5 in the absence of Al. In the absence of added Al, lucerne (Medicago sativa) cv. Hunter River and phalaris (Phalaris aquatica) cv. Grasslands Maru grew better at pH 5.5 than at 4.5. The reverse was so for ryegrass (Lolium perenne) cv. Grasslands Nui and subterranean clover (Trifolium subterraneum) cv. Tallarook. Increasing the pH from 4.5 to 5.5 had no effect on the growth of the other 11 species and cultivars tested. Micromolar activities of Al (<10 8M) markedly reduced the growth of all species. Four groups were tentatively identified in terms of their tolerance to Al. The two cultivars of ryegrass and the two red clover (Trifolium pratense) cultivars, together with Lotus corniculatus cv. Maitland, were relatively sensitive to Al. A 50% reduction in total dry matter (TDM) was associated with {Al3+} of 2-3 8M. The four white clover (Trifolium repens) cultivars, tall fescue (Festuca arundinacea) cv. Grasslands Roa, phalaris cv. Grasslands Maru, and Lucerne cv. Hunter River were intermediate in their tolerance, with {Al3+} of 3-5 8M reducing TDM by 50%. The two subterranean clover cultivars were more tolerant to Al than white clover (50% TDM reduction at 5-6 8M {Al3+}. Lotus pedunculatus CV. Grasslands Maku was exceptional in its tolerance to Al. Its growth was unaffected by {Al3+} up to 5 8M and 50% growth reduction was associated with {Al3+} of 7 8M The results of this study emphasize the need to conduct solution culture experiments at realistic solution concentrations and ionic strengths when examining the effects of Al on plant growth.

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