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1
Thompson, Katherine Hirsch. "Effect of dietary manganese and vitamin E deficiencies on tissue antioxidant status in STZ-diabetic rats." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/32171.
Full textAbstract:
Interactions between manganese (Mn) deficiency and streptozotocin (STZ)-diabetes with respect to tissue antioxidant status were investigated in male, Sprague-Dawley rats. All rats were fed either a Mn-deficient (1 ppm) or a Mn-sufficient (45 ppm) diet for 8 weeks. Diabetes was then induced by tail-vein injection of STZ (60 mg/kg body weight), after which the rats were kept for an additional 4 to 8 weeks. The control groups comprised rats not injected with STZ, which were either Mn-deficient or Mn-sufficient.
The Mn-deficient diet decreased the activities of manganese superoxide dismutase (MnSOD) in kidney and heart, and of copper-zinc superoxide dismutase (CuZnSOD) in kidney, in non-diabetic animals. In the diabetic rats, the Mn-deficient diet induced more pronounced decreases in activities of these same enzymes, and also increased liver MnSOD activity. Pancreas weights were significantly lower in Mn-deficient, compared to Mn-sufficient rats. Also, Mn-deficient, diabetic rats were significantly more hyperglycemic in response to a glucose load than Mn-sufficient, suggesting that they may have been more severely diabetic.
Surprisingly, plasma and hepatic vitamin E levels increased progressively with the duration of diabetes. Lipid peroxidation, as measured by H₂O₂ -induced production of thiobarbituric acid reactive substances in erythrocytes, plasma
lipoperoxides, and renal adipose tissue fluorescence, also increased concomitant with decreased liver and kidney glutathione levels.
The effect of vitamin E-deficiency on Mn-deficient, diabetic rats was also investigated. Predictably, vitamin E-deficient rats were almost entirely depleted of plasma and liver vitamin E after 12 weeks on the deficient diets (4 weeks after STZ treatment). Consistent with this, tissue lipid peroxides were elevated compared to vitamin E-sufficient rats. Superimposing vitamin E-deficiency on manganese deficiency failed to add any further deficits in tissue antioxidant status. Higher glycosylated hemoglobin levels were observed in vitamin E-deficient, compared to vitamin E-sufficient, diabetic rats.
These findings demonstrate for the first time an interactive effect between manganese deficiency and STZ-diabetes resulting in amplification of tissue antioxidant changes seen with either manganese deficiency or STZ-diabetes alone. This effect of cofactor deprivation in experimental diabetes raises the question of adequacy of the nominally Mn-sufficient diet in insulin-dependent diabetes mellitus.Land and Food Systems, Faculty of
Graduate
2
Fullarton, Agneta Carol. "An investigation into the recovery of function after different types of injury and repair of peripheral nerve : a comparison between non-diabetic and STZ diabetic rats." Thesis, University of Edinburgh, 1995. http://hdl.handle.net/1842/20514.
Full text
3
Xiang, Hong. "Effects of myo-inositol and, or triiodothyronine (T₃) treatment on cardiac dysfunction and elevated myocardial lipid levels in STZ-diabetic rats." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26675.
Full textAbstract:
A number of experimental studies have implied a link between diabetes-induced lipid accumulation in the myocardium and the development of cardiomyopathy. Since diabetics excrete large amounts of myo-inositol which is a lipotropic agent, this study was undertaken to investigate the effects of myo-inositol on the elevated myocardial lipid levels and the depressed cardiac performance of diabetic rats. Diabetes was induced in female Wistar rats (190-215 g) with streptozotocin (STZ) (55 mg/kg, i.v.). Three days after diabetes induction, myo-inositol was administered in the drinking water (2.5 g/kg/day) for a 8 week period. Untreated diabetics exhibited a loss of body weight, hyperglycemia, hypoinsulinemia and hypothyroidism. These effects were not altered after myo-inositol treatment. STZ-diabetes also produced a significant elevation of plasma and myocardial triacylglycerol, cholesterol and phospholipid. Myo-inositol treatment decreased these lipid levels. In addition, hearts from diabetic animals had a decreased ability to develop left ventricular developed pressure (LVDP) and both the rate of pressure rise (+dP/dt) and the rate of pressure decline (-dP/dt) were also reduced. Hearts from myo-inositol-treated diabetic animals showed a partial but definite improvement of cardiac function.
As diabetes-induced hypothyroidism was not altered after myo-inositol supplementation, a combination treatment of both myo-inositol (2.5 g/kg/day, p.o. daily) and T₃ (30 ug/kg/day, s.c. daily) was then undertaken to determine whether heart function of diabetic rats could be further improved. STZ-diabetic rats were characterized by a loss of body weight, hyperglycemia and hypoinsulinemia; none of which were altered by either T₃ or myo-inositol plusT₃ treatment. T₃ treatment normalized the thyroid state of diabetic animals as shown by Tahiliani and McNeill (1984). However, plasma and myocardial triacylglycerol, cholesterol and phospholipid levels of diabetic rats either remained elevated or were further increased with T₃ or myo-inositol plus T₃ treatment. In addition, T₃ treatment alone did not prevent cardiac dysfunction in diabetic rats. There was, however, some improvement in heart function in the groups treated with both myo-inositol and T₃, but the improvement was not as pronounced as with myo-inositol treatment alone.Pharmaceutical Sciences, Faculty of
Graduate
4
Lemos, Virgínia Carvalho. "Evaluation of the effect of A. unedo L. extracts on blood indices and microelements status in high-fat STZ-induced diabetic rats." Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/15469.
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Mestrado em Bioquímica - Métodos BiomolécularesThe strawberry tree (Arbutus unedo L.) is an endemic shrub, which grows in the Mediterranean region. In Portugal, it can be found through all the country but mostly in the south. The fruit is used to produce hams and jellies and firewater, reason why the fruit’s chemical composition is relatively well known, when compared to the leaves. Until now, there are only a few studies concerning the leaves’ chemical composition and the existing mainly focus on their phenolic content. This plant has long been used in folk medicine to treat a vast amount of illnesses like gastrointestinal disorders, gastritis, dermatologic and urological problems, kidney problems and cardiovascular diseases. However, very few studies in vivo have been performed about these medicinal properties until now. Diabetes is a pandemic disease that affects millions of people. There are only two studies about A. unedo root’s aqueous extract antidiabetic properties. This study evaluated the anti-diabetic effect of strawberry tree (Arbutus unedo L.) fruit and leaf aqueous extracts in streptozotocin (STZ) induced diabetic rats fed with a high-fat diet. Firstly the optimal parameters to obtain the fruit aqueous extract were established. After that, the leaves and fruits aqueous extracts were characterized in terms of total phenolic content (TPC). Leaf aqueous extracts presented a higher TPC concentration than the fruit aqueous extract. Male Wistar rats were injected with STZ, after a 2 week feeding with high-fat diet in order to induce non-insulin dependent type 2 diabetes. After confirmation of the rats’ diabetic state, the animals were fed with Arbutus unedo L. fruit aqueous extract or leaf aqueous extract added to the high-fat diet for 4 weeks. The extracts were given in the following doses: 1.25g /kg b.w./day for leaf extract and 0.5g /kg b.w./day for fruit extract. After the animals were sacrificed analyses were perform for blood indices, haematological indices, mineral tissular status and gene expression. Arbutus unedo L. fruit extract presented a tendency to lower blood glucose levels. More beneficial effects were seen for this extract in terms of regulating TNF-α gene expression in muscular tissue. Some effects in zinc renal homeostasis were also shown, although they were not very significant. The leaf extract, appeared to be more efficient in regulating insulin levels and improving HOMA-IR index. It also presented a tendency towards lowering blood glucose levels. Both extracts proved to efficiently improve insulin sensitivity (leaf extract in a larger extent). In this study, there were also some good preliminary results, as the groups supplemented with this extracts showed a tendency in decreasing serum creatinine and/or phosphatase concentration, improving the Red Cell Distribution Width measured with standard deviation (RDW-SD) and lymphocytes concentration. Positive antidiabetic and anti-inflammatory aspects were found for both aqueous extracts. The results obtained confirm the mid-therapeutically effects of A. unedo as the plant’s material effectively improved insulin sensitivity (HOMA-IR index) although failed to significantly modulate glucose metabolism. This study was the first in vivo trial on a type 2 diabetes animal model that evaluated the antidiabetic potential of Arbutus unedo L. fruits and leaves aqueous extracts.
O medronheiro (Arbutus unedo L.) é um arbusto endémico que cresce em toda a região Mediterrânica. Em Portugal, pode ser encontrado em todo o território continental, principalmente na zona sul do país. O fruto é utilizado na produção de compotas, marmeladas e aguardente, pelo que a composição química deste é relativamente bem conhecido quando comparado com as folhas. Actualmente, existem poucos estudos sobre a composição química das folhas, sendo que a maioria visa apenas o seu conteúdo em compostos fenólicos. Esta planta tem sido utilizada na medicina popular para tratar diversas doenças tais como distúrbios gastrointestinais, gastrite, problemas dermatológico, urológicos, renais e cardiovasculares. No entanto, até ao momento existem poucos estudos in vivo que atestem estas propriedades medicinais, o que justifica a realização deste tipo de ensaios. No caso da diabetes, que afecta milhões de pessoas em todo o mundo, existem apenas dois estudos sobre as propriedades antidiabéticas do extrato aquoso das raízes de A. unedo. O presente trabalho de mestrado avaliou o potencial antidiabético dos extractos aquosos das folhas e frutos do medronheiro. O estudo foi efectuado em ratos diabéticos (diabetes induzida por Estreptozotocina - STZ) alimentados com uma ração rica em gordura. Primeiramente foram optimizados os parâmetros de extracção para a obtenção do extrato aquosos do fruto. De seguida os extratos aquosos de folhas e frutos foram caracterizados em termos de teor em fenóis totais. O extrato aquoso das folhas apresentou uma maior concentração de fenóis totais do que o extrato aquoso do fruto. Ratos Wistar do sexo masculino foram injectados com STZ após serem alimentados durante 2 semanas com uma dieta rica em gordura. Desta forma induziu-se diabetes tipo 2, não dependente de insulina. Após a confirmação da condição diabética dos ratos, a dieta rica em gordura continuou a ser administrada aos animais. No entanto, as dietas foram suplementadas com extrato aquoso ou do fruto ou das folhas de Arbutus unedo L., num tratamento com a duração de 4 semanas. Os extratos foram administrados nas seguintes doses: 1.25g /kg peso corporal/dia no caso do extrato da folhas 0.5g /kg peso corporal/dia no caso do extrato do fruto. Efectuaram-se análises a parâmetros do sangue, hematológicos, à homeostasia mineral dos tecidos e à expressão de determinados genes após os animais serem sacrificados. O extrato do fruto apresentou uma tendência a regular os níveis de glucose no sangue. Outros efeitos benéficos foram atribuídos a este extrato, nomeadamente ao nível da regulação da expressão do gene de TNF-α a nível muscular. Este extrato também apresentou alguns efeitos ao nível da regulação da homeostase renal de zinco, embora não muito significativos. O extrato da folha provou ser mais eficiente a regular os níveis de insulina, a melhorar a resistência à insulina (HOMA-IR). Também apresentou uma tendência a regular os níveis de glucose no sangue. Ambos os extratos aumentaram de forma eficiente a sensibilidade à insulina (embora o extrato da folha o tenha feito de forma mais pronunciada). Obtiveram-se ainda bons resultados preliminares, uma vez que estes extratos demostraram que ocorreu uma tendência em termos de diminuição da concentração de creatinina e/ou fosfatase, de aumento do valor do parâmetro de Amplitude de Distribuição dos Eritrócitos medido como Desvio Padrão (RDW-SD), de aumento da concentração de linfócitos e diminuíram. Para ambos os extratos foram detectados efeitos antidiabéticos e anti-inflamatórios. Os resultados obtidos confirmam que A. unedo apresenta alguns efeitos terapêuticos, embora ligeiros. Os extratos desta planta melhoraram significativamente a sensibilidade à insulina mas não conseguiram regular significativamente o metabolismo da glucose. Este estudo foi o primeiro, num modelo animal de diabetes tipo 2 que avaliou o potencial antidiabético dos extratos aquosos dos frutos e folhas de Arbutus unedo L.
5
Cameron-Smith, David, and edu au jillj@deakin edu au mikewood@deakin edu au wildol@deakin edu au kimg@deakin. "THE INTERACTION OF DIETARY FIBRE, CARBOHYDRATE METABOLISM AND DIABETES IN THE RAT." Deakin University. School of Health and Behavioral Sciences, 1994. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20040622.171657.
Full textAbstract:
It is currently accepted that the most appropriate diet in the treatment of non-insulin-dependent diabetes mellitus "eNIDDM"e
is high in carbohydrates, high in fibre and low in fat. Dietary fibre reduces the rate of carbohydrate absorption, which may have a beneficial effect on insulin action. Furthermore, high fibre diets also increase the amount of carbohydrates which are not absorbed from the small intestine. These malabsorbed carbohydrates are fermented by the bacterial population in the large intestine, producing short chain fatty acids "eSCFA"e, including propionate, which has been shown to alter liver carbohydrate metabolism. This thesis investigated the actions of slowed carbohydrate absorption and carbohydrate malabsorption in streptozotocin-induced "eSTZ"e diabetic rats.
High carbohydrate diet supplemented with guar gum, a soluble dietary fibre, fed to STZ diabetic rats improved insulin sensitivity. investigation of the alterations in the stomach and small intestine demonstrated that guar increased the viscosity of the meal in the intestine. The action of increased fermentation, producing more propionate, was investigated by supplementing propionate into the diets of STZ diabetic rats or when perfused into isolated rat livers. No changes in insulin action or liver glucose metabolism were measured. in addition, it was shown that guar gum reduces food intake in STZ diabetic rats. Mild reductions in food intake in STZ diabetic rats were shown to increase insulin action.
In summary, STZ diabetic rats fed high carbohydrate, high fibre diets reductions in food consumption and slowed carbohydrate absorption are important factors which may lower blood glucose concentrations and increase insulin action. increased SCFA production is unlikely to contribute significantly to the improvements in insulin action.
6
Sinzato, Yuri Karen. "Análise morfológica e imunológica das placentas de ratas com diabete de intensidade moderada /." Botucatu, 2009. http://hdl.handle.net/11449/106385.
Full textAbstract:
Orientador: Débora Cristina DamascenoBanca: Marilza Vieira Cunha Rudge
Banca: Estela Maris Andrade Forell Bevillacqua
Banca: Renée Laufer Amorim
Banca: Teresa Cristina França Sartori
Resumo: Avaliar os efeitos do diabete moderado nos parâmetros reprodutivos maternos e no desenvolvimento placentário-fetal em ratas Wistar. Metodologia: No dia do nascimento, 147 ratas Wistar foram distribuídas aleatoriamente em dois grupos experimentais: Grupo não-diabético (Controle, n=45) - recebeu o veículo; Grupo diabético (STZ, n=102) - recebeu 100 mg streptozotocin/kg. Na fase adulta, as ratas foram acasaladas e, no dia 0 de prenhez, foram incluídas ratas controle que apresentassem glicemia abaixo de 120 mg/dL e, para o grupo diabete moderado, glicemia entre 120 e 300 mg/dL. Em diferentes momentos da prenhez, glicemia e peso corpóreo foram verificados. No 21º dia de prenhez, as ratas foram anestesiadas para coleta de sangue para dosagem de insulina e, em seguida, foi realizada laparotomia para retirada e pesagem dos fetos e placentas. Os dados maternos e fetais foram analisados por Twoway ANOVA seguida do Teste t. Os recém-nascidos (RN) foram classificados em pequenos, adequados e grandes para idade de prenhez e as comparações entre os grupos foram realizadas segundo o Teste de Qui-quadrado. As ratas STZ apresentaram glicemias maiores nos dias 0 e 14 de prenhez, menor número médio de fetos vivos, implantações e de corpos lúteos, aumento nas taxas de perdas embrionárias pós-implantação, no peso placentário e na proporção de RN pequenos (PIP) e grandes (GIP) para idade de prenhez, redução de RN AIP e inalteração nas concentrações de insulina. Portanto, o diabete de intensidade moderada alterou a glicemia materna no início da prenhez, que deflagrou alterações no organismo materno e/ou no desenvolvimento inicial do embrião, afetando sua implantação e futuro desenvolvimento placentário e fetal.
Abstract: To evaluate the mild diabetes effects on the maternal reproductive outcome and placental-fetal development in female Wistar rats. Methodology: At the birth day, 147 female rats were randomly distributed in two experimental groups: 1) Non-diabetic Group (Control, n=45) - received the vehicle; 2) Diabetic Group (STZ, n=102) - received 100 mg streptozotocin/kg. At the adult phase, the female rats were mated and, at the day 0 of pregnancy, they were included in the control group when presented glycemia below 120 mg/dL and, in the group STZ when showed glycemia between 120 and 300 mg/dL. In different moments of the pregnancy, glycemia and body weight were verified. At day 21 of pregnancy, the rats were anaesthetized to collect blood samples for insulin determination and, soon afterwards, the laparotomy was carried out to withdraw and weigh the fetuses and placentas. The maternal and fetal dates were analyzed by Two-way ANOVA followed by t Test. The newborns (NB) were classified in small, appropriate and large for gestational age and the comparisons between the groups were accomplished according to Qui-square Test. Rats STZ presented higher glycemia at days 0 and 14 of pregnancy, lower numbers of alive fetuses; implantations and corpora lutea; increased rate of embryonic losses, placental weight and proportion of small NB (SGA) and large (LGA); reduced rate of AGA NB and unaltered insulin concentrations. Therefore, the mild diabetes altered the maternal glycemia in the early pregnancy, which caused changes in the maternal organism and/or in the early development of the embryo, impairing its implantation and future placental and fetal development.
Doutor
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Sinzato, Yuri Karen [UNESP]. "Análise morfológica e imunológica das placentas de ratas com diabete de intensidade moderada." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/106385.
Full textAbstract:
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Avaliar os efeitos do diabete moderado nos parâmetros reprodutivos maternos e no desenvolvimento placentário-fetal em ratas Wistar. Metodologia: No dia do nascimento, 147 ratas Wistar foram distribuídas aleatoriamente em dois grupos experimentais: Grupo não-diabético (Controle, n=45) - recebeu o veículo; Grupo diabético (STZ, n=102) - recebeu 100 mg streptozotocin/kg. Na fase adulta, as ratas foram acasaladas e, no dia 0 de prenhez, foram incluídas ratas controle que apresentassem glicemia abaixo de 120 mg/dL e, para o grupo diabete moderado, glicemia entre 120 e 300 mg/dL. Em diferentes momentos da prenhez, glicemia e peso corpóreo foram verificados. No 21º dia de prenhez, as ratas foram anestesiadas para coleta de sangue para dosagem de insulina e, em seguida, foi realizada laparotomia para retirada e pesagem dos fetos e placentas. Os dados maternos e fetais foram analisados por Twoway ANOVA seguida do Teste t. Os recém-nascidos (RN) foram classificados em pequenos, adequados e grandes para idade de prenhez e as comparações entre os grupos foram realizadas segundo o Teste de Qui-quadrado. As ratas STZ apresentaram glicemias maiores nos dias 0 e 14 de prenhez, menor número médio de fetos vivos, implantações e de corpos lúteos, aumento nas taxas de perdas embrionárias pós-implantação, no peso placentário e na proporção de RN pequenos (PIP) e grandes (GIP) para idade de prenhez, redução de RN AIP e inalteração nas concentrações de insulina. Portanto, o diabete de intensidade moderada alterou a glicemia materna no início da prenhez, que deflagrou alterações no organismo materno e/ou no desenvolvimento inicial do embrião, afetando sua implantação e futuro desenvolvimento placentário e fetal.
To evaluate the mild diabetes effects on the maternal reproductive outcome and placental-fetal development in female Wistar rats. Methodology: At the birth day, 147 female rats were randomly distributed in two experimental groups: 1) Non-diabetic Group (Control, n=45) - received the vehicle; 2) Diabetic Group (STZ, n=102) - received 100 mg streptozotocin/kg. At the adult phase, the female rats were mated and, at the day 0 of pregnancy, they were included in the control group when presented glycemia below 120 mg/dL and, in the group STZ when showed glycemia between 120 and 300 mg/dL. In different moments of the pregnancy, glycemia and body weight were verified. At day 21 of pregnancy, the rats were anaesthetized to collect blood samples for insulin determination and, soon afterwards, the laparotomy was carried out to withdraw and weigh the fetuses and placentas. The maternal and fetal dates were analyzed by Two-way ANOVA followed by t Test. The newborns (NB) were classified in small, appropriate and large for gestational age and the comparisons between the groups were accomplished according to Qui-square Test. Rats STZ presented higher glycemia at days 0 and 14 of pregnancy, lower numbers of alive fetuses; implantations and corpora lutea; increased rate of embryonic losses, placental weight and proportion of small NB (SGA) and large (LGA); reduced rate of AGA NB and unaltered insulin concentrations. Therefore, the mild diabetes altered the maternal glycemia in the early pregnancy, which caused changes in the maternal organism and/or in the early development of the embryo, impairing its implantation and future placental and fetal development.
8
Yi-Hui and 顏翊橞. "Anti-diabetic effects of mulberry leaf extract on STZ-induced diabetic rats." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/r768x3.
Full textAbstract:
碩士中山醫學大學
營養學研究所
100
Diabetes Melitus (DM) is one of the most popular chronic diseases in the world. Poor glycemic control is a known major factor that leads to diabetic complications and is attributed to increased oxidative stress. According to previous studies, Morus alba leaves and its extract (ME) possesses hypoglycemic effect with 1-deoxynojirimycin (1-DNJ) as a functional compound. However, the mechanisms underlying which ME and 1-DNJ lowering blood glucose level remain to be clarified. It is also not known how ME and 1-DNJ affect the development of DM nephropathy. The aim of this study is to investigate the antidiabetic effect of ME and 1-DNJ including their effect on the development of diabetic nephropathy and the related mechanisms. Male Wistar rats were injected with streptozotocin (65 mg/kg BW, i.v.) to induce DM. Control rats were injected with citrate buffer solution. 7 days after induction, the DM rats were assigned randomly to six groups and were treated with 1, 3, 10, 30 mg/kg BW of ME, 30 mg/kg BW of DNJ or vehicle (d.d. H2O; 2 ml/kg BW) for a week. Normal control rats also received vehicle. OGTT was carried out at 7 or 11 days and ITT was carried out at 11 days after induction. At 14 days after induction, rats were killed with CO2 and organ/tissue samples collected for various biochemical analyses and the determination of oxidative stress and renal function. On the DM rats all doses of ME and 1-DNJ significantly ameliorated fasting blood glucose and AUC of glucose during OGTT period chronically and ME also showed such effect acutely. All doses of ME and 1-DNJ significantly increased peripheral and pancreatic levels of insulin and improved HOMA-IR in DM rats. Ten and 30 mg/kg of ME improved insulin-induced lowering of blood glucose level in DM rats. In the other hand, all doses of ME and 1-DNJ significantly reversed increased TBARS of the pancreas, kidneys, livers, muscles and peripheral blood in DM as well as decreased GRd activity of the pancreas and kidneys in DM. In addition, 1-DNJ significantly reversed lowered GPx and GRd activity of the livers and muscles in DM. ME at 30 mg/kg significantly reversed lowered GPx activity of the muscle, at 10 to 30 mg/kg significantly reversed lowered GRd activity of the kidneys, at 1 mg/kg significantly reversed lowered GRd activity of of the livers, at 3 to 30 mg/kg significantly reversed lowered GRd activity of the muscles. All ME doses and 1-DNJ significantly decreased NO content of the pancreas, kidneys, livers,and muscles in DM. The indices of renal function, including BUN, CCR, and GFR were all ameliorated by all doses of ME and 1-DNJ. In addition, 30 mg/kg of ME and 1-DNJ improved glomerular morphology of the kidneys and lowered blood pressure in DM. In conclusion, ME and 1-DNJ improved insulin secretion and insulin sensitivity and attenuated the development of diabetic nephropathy. The antidiabetic effects of ME and 1-DNJ is at least partly due to the amelioration of the increased oxidative stress in DM.
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Yao, Jun. "Effects of vanadium compounds on diabetes induced changes in STZ-diabetic rats." Thesis, 1996. http://hdl.handle.net/2429/4473.
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Controversial reports on the efficacy and possible toxicity of vanadium obtained
from various studies may be attributed to differences in the method of diabetes
induction and/or to differences in animal strains. The objective of this study was to
evaluate the contribution of these two factors to the effects of vanadium in the
treatment of diabetes. Two methods of streptozotocin (STZ) induced diabetes in
rats have been used for studying the antidiabetic effects of vanadium. One involves
a single intravenous (i.v.) injection of 60 mg/kg STZ, and the other uses two
subcutaneous (s.c.) injections of 40 mg/kg STZ, to either Wistar or Sprague Dawley
(SD) rats. In a 7-week chronic study, SD rats appeared to develop a more severe
diabetes (indicated by higher plasma cholesterol and higher fasting plasma glucose
levels) following the single i.v. injection of STZ than rats made diabetic by two s.c.
injections of STZ. Irrespective of the method of diabetes induction or the strain of
rat, the responses of all the diabetic animals to vanadyl sulphate treatment were
similar. In a 1-week acute study, Wistar diabetic rats were more responsive than
SD diabetic rats to vanadyl sulphate and to lower doses (0.6 and 0.8 mmol/kg) of a
new organic compound, bis(maltolato)oxovanadium(IV).
10
Chen, Tai-You, and 陳泰佑. "Effects of Cordyceps sinensis on Blood Glucose in STZ-induced Diabetic Rats." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/89246554680918450648.
Full textAbstract:
碩士國立中興大學
獸醫學系暨研究所
101
In recent years, researches indicate diabetes implicated in metabolic syndrome. There are sulfonylurea, bioguanides, alpha-glucosidase inhibitor, thiazolidinediones, insulin, dipeptidyl peptidase-4 inhibitors and so on in diabetic drugs which have their indication and a lot of side effects. Therefore, many researchers develop drugs or extraction for natural products to improve metabolic syndrome and control diabetes, and moreover can reach prevention. Cordyceps has a large amount of biological ingredients which increase numbers of transporter proteins and stablelize concentration of blood glucose. Therefore, we found Cordyceps extract could prevent hyperglycemia in STZ-induced diabetic rats.
11
Chiu, Lan Chi, and 邱嵐琪. "Antihyperglycemic and antioxidative potential of Psidium guajava in STZ-induced diabetic rats." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/23669297627376652603.
Full textAbstract:
碩士亞洲大學
保健營養生技學系碩士班
98
Psidium guajava (PG) is an important tropical fruit, widely consumed fresh and also processed (beverages, syrup, and jams), and rich in vitamin C and flavonoids. Several studies suggested that the extract from PG leaves possesses antidiabetic effects in diabetic mice model and these effects are speculated to account its polyphenolic compounds. Oxidative stress play a crucial role in the development of diabetic complications The aims of this study were to investigate whether PG could reduce hyperglycaemic and ameliorate oxidative stress of the streptozotocin (STZ; 55 mg/kg, i.p.) -induced diabetic rats. Male Sprague-Dawley (SD) rats were divided into four equal groups (n=13): control, diabetes, orally with different doses of PG (120 and 250 mg/kg-2d for 4 weeks; LPG and HPG) diabetic rats from one week after STZ administration. Fasting blood glucose levels, serum insulin, lipid peroxidation (malondialdehyde), DNA breakage, reduced glutathione (GSH), GSH peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) levels were measured, and immunohistochemical studies were performed in all of the groups. The obtained data showed that STZ resulted in oxidative stress and affected the antioxidant status. Treatment with different dose of PG significantly reduced fasting hyperglycemia and oxidative stress, and augmented the antioxidant system. In histological investigations, PG treatment protected the majority of the pancreatic islet cells, with respect to the control group. As a result, PG exhibited significant antihyperglycemic effect and protected beta-cells in STZ-diabetic rats, in a dose-dependent manner, and diminished the hyperglycemia-related oxidative stress.
12
Tsai, Ling-Jane, and 蔡玲貞. "The Effects of L-arginine on nephropathy in STZ-induced diabetic rats." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/44803321786962232108.
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碩士中山醫學院
營養科學研究所
87
Diabetic nephropathy is a major complication in diabetes mellitus (DM). There are a lot of evidences showe that the hyperglycemia induced non-enzymatic glycated proteins are related to the diabetic nephropathy. Recent studies pointed out that aminoguanidine (AG) can inhibit the occurrence of proteinuria, the thickening of the glomerular basement membrane, and the synthesis of the advanced glycosylation end products (AGEs) in streptozotocin (STZ)-induced DM rats. The functional group of L-arginine is similar to that of AG. In addition, being the known precursor of nitric oxide，L-arginine may alleviate the damages of glomerular via the production of NO. The aims of the present study are to investigate the relationship between high levels of arginine intake on renal function and glycated proteins in DM. After administered with STZ, rats were divided into three groups, that is, rats provided with distilled water (DC group), water containing 2% glycine (DG group), or water containing 2% L-arginine (DA group) as their drinking water. Three control groups administered with saline injection were also included, that is, rats provided with distilled water (CC group), water containing 2% glycine (CG group), or water containing 2% L- arginine (CA group) as their drinking water. Rats were free access to diet and water for 8 or 16 weeks and then sacrificed after 24h urine collection. Renal function and nutritional status were observed. Plasma was collected and the weights of kidney and skeletal muscle were obtained. The levels of glycated proteins in plasma were measured by NitroBlueTelrazolium (NBT test) using spectrophotometer. The levels of plasma substrates were measured by automatic analyzer. The results of the present study showed that STZ injection increased the excretion of protein in the urine in rats fed with distilled water (DC group) and glycine contained water (DG group) for 8 or 16 weeks, whereas L-arginine supplementation (DA group) improved the STZ-induced proteinuria (p<0.05). Arginine supplementation also improved the nutritional status, for example, the levels of plasma albumin was significantly greater in DA group (p<0.05) compared with that of in DC and DG groups, but not significantly different from that of in CC group. The loss of skeletal muscle was also improved by arginine. After 16 weeks, the glomerular filtration rate (GRF) was significantly increased in DC and DG groups, even to the conditions with hyperfiltration. There was no significant difference in GRF between DA and CC groups. The levels of plasma glucose were also improved in DA group. However, all of the three STZ injected groups were tended to have greater levels of glycated proteins and lipid in the plasma. Supplemental L-arginine (DA group) did not improve the STZ induced protein glycosylation in rats. The results of the present study suggested that high levels of L-arginine intake might improve the process of the diabetic nephropathy, protect the renal function, and reverse the DM caused nutritional loss. These functions of supplemental L-arginine might not due to the inhibition of the protein glycosylation. Supplemental glycine did not improve the diabetic nephropathy as what arginine did.
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Goche, Montes Danitza Eustolia. "Effects of restraint stress on hypothalamic-pituitary-adrenal activity in STZ-diabetic rats." 2005. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=370458&T=F.
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CHENG, YU-MING, and 鄭昱明. "The Long-Term Influences of Polyphenolic Compounds on the STZ-Induced Diabetic Rats." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/51719701132003619121.
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碩士弘光科技大學
食品暨應用生物科技所
99
Hyperglycemia increases oxidative stress (OxS). Sustained hyperglycemia is the underlying factor for diabetic mellitus (DM) complication. OxS plays a major role in the aetiology and pathogenesis of cataract and renal carcinoma formation in the DM patients. This study evaluated the effect of a long-term polyphenolic compounds therapy on the STZ-induced DM rats. Briefly, SD rats were given ip STZ (50mg/kg bw) to induce type 1 DM. Rats having blood sugar level over > 230mg/dL were selected for treatment with various polyphenolic compounds including gallic acid, rutin, EGCG, ferulic acid and quercetin at 70 mg/kg bw. The total course extended for 28 weeks. Finally evaluate the blood sugar and other physiological effect of phenol compound. Results indicate that only quercetin was moderately hypoglycemic and potent insulin secretagougue. Furthermore, quercetin also inhibited at certain degree the formation of serum malondialdehyde (MDA) and increased the level of glutathione (GSH). The HE stained specimen also revealed that only quercetin had certain degree of protective effect on lesions of oculus. However, quercetin was shown to aggravate renal carcinoma (RCA). Further, quercetin repress kidney p53, Bax and Bad expression in western blot, which may close relation with its initiation renal carcinoma. Conclusively, quercetin has prevention cataract effect which may be attributed to its hypoglycemic and insulin secretagougue bioactivity, and some due to increased GSH level effect. Conversely, quercetin is carcinogenic to renal cells. The action mechanism of quercetin in aggravating RCA may be due to its inhibition of apoptoic proteins. Key words :Diabetes, Polyphenolic compound, Renal carcinoma
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KU, HUI-HSUAN, and 古慧萱. "Hypoglycemia Effect of Containing Guava Leaf Extract Drink in STZ Induced Diabetic Rats." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/fw9ezw.
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碩士實踐大學
食品營養與保健生技學系碩士班
106
The International Diabetes Federation pointed out that in recent years, the number of people suffering from diabetes worldwide has increased year by year due to aging or long-term abnormal diet such as high-sugar and high-fat diet. Guava leaf extract (GLE) has been indicated the ability of anti-hyperglycemic and anti-hyperlipidemic, probably due to its phenolic content. The purpose of this study is to investigate the effect of hypoglycemia activities of drink containing GLE on high fat high fructose diet rats which is intraperitoneally injected by Streptozotocin (STZ) and Nicotinamide (NA) to induce diabetes. This study use 60 male Sprague-Dawley rats and divide them into 2 groups, one is Normal control group and the other is diabetes-induced group. After the success of inducing diabetes by STZ and NA, the diabetes-induced group are divided into 5 groups, diabetes mellitus group (DM), positive control group (PC), low-dose GLE group (L), medium-dose GLE group (M), and high-dose GLE group (H) respectively. These 5 groups are given GLE for 5 weeks then sacrificed. In the experiment, we test the body weight, diet intake, water intake, fasting blood glucose (FBG), and fasting blood insulin of rats, and do oral sucrose tolerance test (OSucTT) on rats. After sacrificing, we also test blood biochemical value, antioxidant enzyme activity , inflammation, and gene expression in intestine tissue. Results showed that supplement GLE were significant decrease FBG, OSucTT, the area under the blood glucose curve in OSucTT, inflammation, expreesion of Sodium-Dependent Glucose Cotransporters-1（SGLT 1）, Glucose Transporter-2（GLUT 2）and increase antioxidant enzyme activity, TBARS compare with DM group. In conclusion, GLE in this study could improve blood glucose, inflammation, oxidative stress,α-glucosidase inhibitor on hyperglycemic diabetes rats.
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盧中庸. "Effects of Interferon (IFN) —γ on renal fibroblasts and streptozotocin(STZ)-diabetic rats." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/78248119410276756639.
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碩士高雄醫學大學
生物化學研究所
90
The pathogenesis of diabetic nephropathy (DN) includes hyperglycemia, advanced glycation end-product (AGE), transforming growth factor- (TGF-) and angiotensin II, etc. Chronic DN is characterized by the presence of renal fibrosis including tubulointerstitial fibrosis. Thus, interstitial fibrosis may be the final common pathway of DN. Renal fibrosis is a complex process characterized by an accumulation and a decreased rate of degradation of of extracellular matrix. Cytokines such as TGF-β and angiotensin II are two of the most important players in these processes. Moreover, fibroblasts are thought to be the main effector cells in renal fibrogenesis. Unfortunately, therapeutic options to retard the progression of DN are still imperfect therapy in general is confined to the control of blood pressure (e.g. angiotensin-converting enzyme inhibitor such as captopril) and hyperglcaemia in diabetic subjects.. However, there is still no specific therapy to halt progressive interstitial fibrosis in DN. In this regard, our laboratory has shown that AGE increased proliferation of renal fibroblast (NRK-49F) cells by activating the Jak (Janus kinase)2/STAT (signal transducers and activators of transcription) 1,3,5 pathway. Interferon- (IFN-), which activates the STAT1 homodimer, has been shown to be effective in skin and lung fibrosis. Thus, the purpose of this study was to elucidate the effects of IFN- on models of DN in NRK-49F cells and Streptozotocin-diabetic rats. We found that IFN- resulted in a dose- and time-dependent inhibition of proliferation and collagen production in NRK-49F cells. IFN-（1000 U/ml）also reversed high glucose-, AGE and angiotensin II-induced proliferation and collagen synthesis in these cells. Interestingly, IFN- partially reversed TGF-β-induced hyperprophy (but not growth inhibition) and collagen production in these cells. Additionally, AG490（a specific JAK2 inhibitor）and STAT1 decoy ODN were effective in partially reversing IFN--induced growth inhibition. We also found that IFN-increased cyclin-dependent kinase inhibitors （p21WAF/CIP1 and p27Kip）protein expression. Additionally, IFN- reversed high glucose, AGE and angiotensin II-induced inhibition of p21WAF/CIP1 and p27Kip protein expression in NRK-49F cells. Streptozotocin-diabetic rats developed proteinuria (12633 mg/day) at 15th day, while insulin and IFN-treatment normalized urinary protein levels in diabetic rats (599 and 6013 mg/day, P<0.05, respectively). We concluded that IFN- partially reversed high glucose, AGE and angiotensin II-induced proliferation, collagen synthesis and inhibition of p21WAF/CIP1 and p27Kip protein expression in NRK-49F cells. This reversing effect may be mediated by the JAK2/STAT1 pathway. Thus, activation of STAT1 only (by IFN-) and simultaneous activation of STAT1,3,5 (by AGE) have opposing effects in terms of cellular growth and collagen production in NRK-49F cells. Moreover, IFN-improvedproteinuria in diabetic rats at 15th day.
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Ching, Ho Ching, and 何菁菁. "Effect of dehulled adlay on lipid and carbohydrate metabolism in STZ-induced diabetic rats." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/35350088432745727399.
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碩士國立海洋大學
食品科學系
88
Abstract The purpose of this research was to investigate the effects of dehulled adlay on lipid and carbohydrate metabolism in streptozotocin (STZ)-induced diabetic rats. Male Sprague Dawley (SD) rats were used as experimental animal model and were made diabetic status by intravenous injection of 60 mg per kg of streptozotocin. In experimentⅠ, the effects of dehulled adlay and cholesterol on lipid and carbohydrate metabolism in diabetic rats. The experiment lasted six weeks and divided into 4 groups: (1) Corn starch (2) Dehulled adlay (3) Corn starch + 0.5% cholesterol (4) Dehulled adlay + 0.5% cholesterol. The results of experiment Ⅰ showed that diabetic rats fed dehulled adlay groups had significantly heavier body weight, lower volumne of drinking-water and urine than corn starch groups. Dehulled adlay groups had higher liver weight and had also more adipose tissue weight and relative adipose weight, shorter length of small intestine, lighter cecal weight and cecal content, fewer amount of total short-chain fatty acids in cecal content. In addition, dehulled adlay decreased plasma glucose, fructosamine and lactate levels in diabetic rats. In cholesterol-fed groups, dehulled adlay groups had markedly decreased plasms TBARS levels and C/P ratio. In cholesterol-free groups, dehulled adlay couled decrease plasma cholesterol levles by 22%. In cholesterol- fed groups, dehulled adlay couled decrease plasma cholesterol levels by 72%. Moreover, dehulled adlay couled reduce plasma VLDL-C, LDL-C and increased HDL-C/TC ratio. Dehulled adlay also decreased plasma triglyceride, VLDL-TG, LDL-TG, phospholipid, apolipoprotein B, free fatty acid, β-hydroxybutyrate levels compared to corn starch diet. The addition of cholesterol to dehulled adlay further increase the levels of cholesterol , and significantly decreased the activity of fatty acid synthase in the liver. Diabetic rats fed dehulled adlay diet had higher fecal cholesterol and triglyceride contents. Experiment Ⅱ was to compare the effect of dehulled adlay with other frequently used carbohydrates (corn starch and rice) on the physiological funtion of diabetic rats, The experiment lasted nine weeks and divided into 3 groups: (1) corn starch (2) dehulled adlay (3) rice. The results of experiment found that dehulled adlay group significantly increased the adipose tissue weight and decreased plasma free fatty acid levels in diabetic rats, implicating that dehulled adlay prevents the decomposition of adipose tissue. In the fourth week, dehulled adlay had significantly lower plasma glucose levels than corn starch groups, but lasted to the ninth week the difference was not significant. From the results of the oral glucose tolerance test showed that diabetic rats fed dehulled adlay had better glucose tolerance ability. Dehulled adlay group and rice group were apparently reduced plasma total cholesterol, phospholipid, IgA, IgG levels compared with corn starch group. Rat fed dehulled adlay had lower plasma VLDL-C+LDL-C and free cholesterol levels, and higer HDL-C/TC ratio. Although diabetic rats fed the dehulled adlay had higher liver weight, increased cholesterol levels and total activity of G-6-P DHase in the liver. On the other hand, dehulled adlay feeding couled reduce plasma lactate and liver glycogen levels and increase total activity of hexokinase, PFKase and specitic activity of G-6-Pase in the liver.
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Wu, Wei-Hao, and 吳唯豪. "The anti-neuroinflammatory effects of a synthetic marine-derived compound on STZ induced diabetic rats." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/dawxz5.
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碩士國立中山大學
海洋生物科技暨資源學系研究所
102
Diabetes mellitus is a metabolic disease that can have long-term complications, including diabetic neuropathy pain. Base on preliminary screening, we had found that a marine-derived compound, SWV-1 has potential in vitro anti-inflammatory effects. In present study, we found that intrathecal or oral SWV-1 produce antinociceptive effects in STZ-induced diabetic neuropathic rats. Moreover, compare with ziconotide, SWV-1 did not produced any obvious adverse effects. Immunohistochemistical analyses also showed that SWV-1 significantly attenuated STZ-induced spinal neuroninflammation. We further demonstrated that administration of TGF-β type I receptor inhibitor attenuate the analgesic effect of SWV-1 in STZ-rats. In conclusion, SWV-1 is a potential candidate compound for drug development to treat neuropathic pain in patients with diabetes.
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Hsu, Shu-hui, and 許淑惠. "Effect of Pu-erh tea on blood sugar and lipid in STZ-induced diabetic rats." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/52w3gp.
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碩士靜宜大學
食品營養研究所
97
Pu-erh tea is a fermented tea produced of the Yunan district in China. Present pharmacology study supported that Pu-erh tea has a affect of decreased blood glucose and lipids, but mechanism also not clear. Diabetes mellitus was the 5th of top 10 cause of death in Taiwan, therefore avoid complications of blood glucose and lipids exceptional is very important. The aim of this study was to determine the effect of Pu-erh tea treatment on blood glucose and lipids in STZ-induced diabetic rats of prevent and intervene. In the experiment I (prevention experiment), rats are divided into five groups: (1) control, (2) diabetic group with intraperitoneal injection of streptozotocin (65 mg/kg B.W.) and nicotinamide (60 or 120 mg/kg B.W.) [DM(60N) and DM(120N)], (3) diabetic group with oral Pu-Erh tea (0.1g/kg B.W.) 14 days before intraperitoneal injection of streptozotocin (65 mg/kg B.W.) and nicotinamide (60 or 120 mg/kg B.W.) [DM-TP(60N) and DM-TP(120N)], all groups experiment period for 29 days. The results showed that DM(60N) group has seriously syndrome, but in DM-TP(60N) group can significantly prevent triglyceride and free fatty acid concentration and DM-TP(120N) group has effect of plasma glucose, insulin, triglyceride and free fatty acid concentration. In the experiment Ⅱ (intervention experiment), rats are divided into six groups: (1) control, (2) diabetic group with intraperitoneal injection of streptozotocin (65 mg/kg B.W.) and nicotinamide (120 mg/kg B.W.) [DM(120N)], (3) diabetic group with oral Pu-erh tea (0.1g/kg B.W. and 0.2g/kg B.W.) after diabetic induced [DM-TI1(120N) and DM-TI2(120N)], (4) diabetic group with oral Gibenclamide and Simvastatin (10mg/kg B.W.) [DM-glib(120N) and DM-statin(120N)] , all groups experiment period for 22 days. The results showed that DM-TI2(120N) group has effective reduced diabetic rats plasma glucose, insulin, triglyceride and free fatty acid concentration, changed rats clinical syndrome like polydipsia, polyuria and urine glucose, moreover inhibited epididymal fat pad tissue lipolysis. There was no significantly effected in plasma glucose and triglyceride of DM-TI1 group, but other data has same results of DM-TI2(120N) group. In summary, Pu-erh tea can improve significantly plasma glucose, insulin, triglyceride and free fatty acid concentration in rats before and after diabetic induction. Furthermore, Pu-erh can significantly improve the clinical syndrome and fat tissue weight. The efficacious components or mechanism of Pu-Erh tea needs futurer study.
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LIAO, WAN-RU, and 廖婉如. "Effect of Functional Components from Eureka Lemon on Blood Sugar Content of STZ-induced Diabetic Rats." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/2zvvvn.
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碩士弘光科技大學
食品科技所
107
The prevalence of increasing obesity status in the global population leads to rise in type 2 diabetes. Diabetes mellitus is one of the most common and serious metabolic diseases worldwide. The therapy of Type 2 Diabetes Mellitus stays a challenging issue. During the last decade, there has been an interest in the expansion of anti-diabetic drugsespecially those of natural sources. Exocarp contains Limonin, Phenolic acid compounds, Flavonoids, essential oils, dietary fibers and various active composition. These composition are useed to prevent gastrointestinal diseases, chronic diseases such as obesity, diabetes, cardiovascular diseases, cancer etc. Therefore, the reuse of discarded exocarp has into focus in recent years. In this study, we analyze the different maturity Eureka Lemon functional ingredients, and to explore the efficacy of blood glucose regulation. The results show that: the yield of essential oils of Eureka Lemons from different maturity. Essential oil of whole fruit content is between 0.37% ~ 0.64%, exocarp content is between 1.52% ~ 2.89%,and main component is the limonene of Monoterpenes, whole fruit content is between 40.95% - 55.85%, exocarp content is between 43.97% - 60.57%. On the other hand, there are also functional compounds of Eureka Lemons. Phenolic compounds are found in greatest abundance in Eureka Lemons of extract waste water, 1 month of whole fruit which contains 433.54 mg/100 g, 1 month of exocarp which contains 448.97 mg/100 g. Flavonoids are found in greatest abundance in Eureka Lemons of extract waste water, 1 month of whole fruit which contains 475.94 mg/100 g, 1 month of exocarp which contains 1265.65 mg/100 g. Among them, hesperidin is the main flavonoid compound, and the whole fruit and exocarp are respectively contained 169.54 ~ 352.06 mg/100 g and 89.75 ~ 870.15 mg/100 g, respectively. Limonoids are found in greatest abundance in Eureka Lemons of 1 month of FWF 35.31 mg/100 g, 2 Months of WBWE 20.40 mg/100 g. Limonin are found in greatest abundance in Eureka Lemons of 4 Months of FWF 39.86 mg/100 g, 1 month of WBE 217.97 mg/100 g. Total polyphenolics are found in greatest abundance in extract waste water of 1 month Eureka Lemons methanol extract was hightest, whole fruit which ontains 5.45 mg FAE /g, exocarp which contains 4.50 mg FAE /g.Total flavonoids are found in Eureka Lemons of extract waste water methanol extract was hightest, 2 month of whole fruit which contains 101.42 mg HE /g, 1 month of exocarp which contains 109.95 mg HE /g. Also for the scavenging capacity of ABTS and DPPH, the extract waste water of Eureka Lemons using methanol extract gave best results. Investigation of the effect of Eureka Lemon, essential oil and flavonoids of extract waste water of exocarp on animal's hypoglycemic effects. After fed 12 weeks, compared to the DM group on flavonoids of extract waste water of exocarp groups had 2.86 ~ 9.38% higher body weight, fed on essential oil groups had 6.90% ~ 7.69% less body weight. For blood sugar, groups of induced NA / STZ as compared to the DM group relative reduction 33.52%. The Insulin, fed on flavonoids of extract waste water of exocarp groups had relative increase 59.53% ~ 67.77%, essential oil groups had relative increase 51.88% ~ 61.30%; as for HbA1c fed on flavonoids of extract waste water of exocarp groups had relative reduction 36.67% ~ 39.15%, essential oil groups had relative reduction 34.94% ~ 36.34. Fed flavonoids of extract waste water of exocarp groups and essential oil groups compared to the DM group, in ALT relative reduction 34.06%, as for BUN relative reduction21.58%. There were significant differences in the concentration of TBARs and changes in enzyme activity. But, in histopathological changes of Kidney and Pancreas found NA / STZ induced diabetes rats can cause damage it is inevitable. Based on our results, we find that feeding on Eureka Lemon, flavonoids of extract waste water of exocarp and essential oil is an effective way of hypoglycemic effects of NA / STZ induced Wistar rats.
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Irem, Mueed. "Role of altered signalling pathways in abnormal vasoconstrictor responses in mesenteric arteries from STZ-diabetic rats." Thesis, 2005. http://hdl.handle.net/2429/17055.
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Cardiovascular complications are recognized to be the major cause of morbidity and mortality associated with diabetes mellitus. One of the most common features of vascular dysfunction in well-established diabetes is the enhanced reactivity of blood vessels to vasoconstrictors. We and others have consistently found that contractile responses of arteries from rats with well-established diabetes to stimulation of G-protein coupled receptors (GPCRs), such as ai-adrenoceptor (α₁-AR) and endothelin-1 (ET-1) receptors, are enhanced. Previous studies from this lab have demonstrated that the increased contractile responses of arteries from chronic (12-14 weeks) streptozotocin- (STZ-) diabetic rats to α₁-AR stimulation result from a change in the signal transduction process downstream from the receptor. Protein kinase C (PKC) has been suggested to contribute to enhanced contractile responses of arteries from STZ-diabetic rats to stimulation of GPCRs. This was investigated in the present study by comparing the effects of the PKC inhibitors, Ro-318220 and calphostin C on contractile responses of mesenteric arteries from diabetic and age-matched control rats to the α₁-AR agonist, norepinephrine (NE) and to ET-1. Since translocation of PKC to the membrane is considered a hallmark of its activation, the effects of NE and ET-1 on particulate (membrane) levels of three isoforms of PKC (PKCα, δ and ε) that have been implicated in contraction were determined. The effect of NE on phosphorylation of CPI-17, a substrate for PKC, was also investigated. Contractile responses of endothelium-denuded arteries from diabetic rats to NE were enhanced, but were normalized by the PKC inhibitors. In contrast, no change in contractile responses of diabetic arteries to ET-1 could be detected, and PKC inhibition attenuated ET-1 responses to a similar extent in both control and diabetic tissues. NE produced a small translocation of PKCε in control arteries, but a significant translocation of PKCα and a much larger translocation of PKCε in diabetic arteries. ET-1 increased translocation of PKCα, δ and ε to the same extent in both control and diabetic arteries. NE significantly enhanced CPI-17 phosphorylation in diabetic, but not control, arteries and this was blocked by calphostin C. In addition to PKC, the RhoA/Rho kinase (RhoK) signaling pathway is also believed to play an important role in vasoconstriction. In the next part of this study, the role of RhoK in enhanced contractile responses of diabetic mesenteric arteries to stimulation of α₁-ARs was investigated. The selective RhoK inhibitors, Y-27632 and H-1152, produced greater inhibition of contractile responses to phenylephrine (PE) in diabetic than control mesenteric arteries and normalized the difference between them. Contractile responses to ET-1 were not different between control and diabetic arteries, and were not affected by RhoK inhibition. Since translocation of RhoK from the cytosolic to the membrane fraction is considered a marker of enzyme activity, the effects of PE on particulate levels of the two RhoK isoforms (ROCK I and II) were determined. A maximum concentration of PE produced significant translocation of ROCK I and II that was inhibited by Y-27632 in both control and diabetic arteries. The PE-induced translocation of ROCK II was significantly greater in diabetic tissues. PE also produced significant translocation of PKCα, δ and ε in diabetic but not control arteries. Y-27632 blocked the translocation of these isoforms in diabetic arteries but had no effect on ET-1 induced translocation of PKC isoforms in control arteries. These data suggest that increased activation of the PKC/CPI-17 as well as the RhoK pathway contribute to the enhanced contractile responses of diabetic mesenteric arteries to α₁-AR stimulation. They further suggest that there is an interaction between the RhoK and PKC pathways on stimulation of α₁-ARs in diabetic mesenteric arteries, and that RhoK may be upstream of PKC. On the other hand, RhoK does not appear to contribute to contractile responses to ET-1 in either control or diabetic mesenteric arteries; this may be the reason why vasoconstrictor responses to this agonist are not altered in diabetic arteries.Pharmaceutical Sciences, Faculty of
Graduate
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Li, Kun-Chen, and 李昆錚. "Antioxidative activities and the effect on glucose metabolism in STZ-induced diabetic rats of Ficus formosana." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/41110436920981289012.
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碩士中國醫藥大學
中國藥學研究所碩士班
97
The aim of this study was to examine the possible antioxidant activities of the water extracts of the fruit, leaf, stem and root of Ficus formosana from Taiwan. ABTS radical monocation scavenging, DPPH radical scavenging, reducing power method, total polyphenol content, total flavonoid content and total flavonol content methods were employed. The result showed that among the four parts of Ficus formosana, the polyphenol content was the highest in the fruit, equivalent to 270.83 mg/g of catechin. The flavonoid content was the highest in the fruit, equaling to 83.79 mg/g of rutin. In the oxidation resistance ABTS free radical scavenging analysis, the TEAC (trolox equivalent antioxidant capacity) values of the water extracts decreased in the order of fruit > leaf > stem > root. In DPPH free radical scavenging as well as in the reducing power experiment, the fruit extract showed the highest oxidation resistance ability. From the above findings, the fruit aqueous extract was found with the highest antioxidant ingredients among the four aqueous extracts of Ficus formosana. Therefore the fruit of Ficus formosana may be a good natural resource for anti-oxidation. In the secondary experiment, stable and chronic non-insulin dependent diabetes mellitus (NIDDM) was induced in animal models, and the antihyperglycemic effect of Ficus formosana fruit was evaluated. Four week old SD rats were used in this experiment. The rats were intraperitoneally injected with streptozotocin (STZ) and nicotinamide. STZ and nicotinamide solutions were diluted by dissolving in 0.1M citrate buffer (pH=4.5). The diabetic rats were divided into six test groups when they were 5 weeks old. The rats exhibited high plasma glucose beyond 130 mg/dL after they were treated with nicotiamide 20mg/kg and STZ 15mg/kg daily for one week. The plasma glucose concentration of the rats administered with FFL, FFM and FFH were significantly decreased as compared to the STZ control group. HbA1c concentration in the nine week old rats that were treated with Ficus formosana fruit decreased significantly in comparison with the STZ control group (p<0.05). Immunohistochemistry examination revealed the presence of β-cells in pancreatic islets, confirming that the hyperglycemia was due to type II diabetes mellitus. The triglycerides, LDL, and cholesterol were significantly decreased as compared to the STZ control group. These results suggested that insulin might provide alleviative effects against diabetic deterioration and hyperlipidemia.
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Kuo, Chun-Cheng, and 郭俊成. "The Effects of Berberine on Insulin Secretion in High-Fat Diet- and STZ-Induced Diabetic Rats." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/58798698699241605706.
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碩士國立中興大學
獸醫學系暨研究所
97
Berberine is a nature compound extracted from Rhizoma Coptidis and was used for the treatment of diabetes for more than 1000 years in Chinese medicine. In order to find a more effective drug to treat diabetes, berberine was used to perfuse rat pancreas and to explore the effect of berberine treatment in high-fat diet- and STZ-induced diabetic rats. The results shown that berberine stimulated insulin secretion in a biphasic manner. In addition, berberine improved oral glucose tolerance test in high-fat diet- and STZ-induced diabetic rats. Berberine protected the effect of glucose-induced insulin secretion in STZ-induced diabetic rats, and increased the sensitivity of glucose-induced insulin secretion in high-fat diet rats. By using rat pancreatic perfusion technique, we found that berberine temporally stimulated insulin secretion, then inhibited insulin secretion. In addition, berberine suppressed insulin secretion and raised blood glucose concentration in fasting SD rats. After rat pancreas was first perfused with propranolol, we found that the action of berberine in insulin secretion may be mainly mediated through beta-receptor activity. Nevertheless, berberine suppressed insulin secretion still need a further study.
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Cheng, Huei-Ju, and 鄭惠如. "Effects of rice bran oil on glycemic control and lipids metabolism in STZ-induced diabetic rats." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/06641387947428171104.
Full textAbstract:
碩士臺北醫學大學
保健營養學系
92
This study was designed to investigate the effects of rice bran oil (RBO) on glycemic control and lipid metabolism in STZ-induced diabetic rats. Diabetes was induced by nictotinamide and streptozotocin injection. The formulas of these experimental diets were modified from the AIN-76 formula. Forty 10-week old male Wistar rats were divided into four groups: a normal rat group fed soybean oil (SBO)-containing diet (SN) or RBO-containing diet (RN) and a diabetic rat group fed SBO-containing diet (SD) or RBO-containing diet (RD). Fasting blood sampling was conducted at zero time, and at the end of the second and the fourth week in this experiment. At the end of this experiment, all rats were sacrificed, and their livers and epididymal fat pads were collected. In this study, STZ-induced diabetic rats showed hyperlipidemia. Whether normal or diabetic rats fed the RBO-containing diet group, the plasma total cholesterol, triglycerides and low-density lipoprotein cholesterol were significantly lower compared with these fed SBO-containing diet group at week 4 (RD < SD and RN < SN)；(P<0.0001). As to plasma high-density lipoprotein cholesterol, RD and RN were significantly higher compared with SD and SN, respectively (P<0.0001). Liver triglycerides were reduced in RD compared with SD (P=0.0235). Whether normal or diabetic rats fed the RBO-containing diet group, the 18:2, 18:3 and total polyunsaturated fatty acids (PUFAs) levels as percentages of total plasma fatty acids were significantly lower comparison with fed the SBO-containing diet group (RD < SD and RN < SN)(P<0.05). In conclusion, consumption of RBO could reduce plasma TG, TC, LDL-C, 18:2, 18:3 and total PUFA levels and increase HDL-C concentrations in normal and diabetic rats. In diabetic rats, consumption of RBO could improve glycemic control and decrease liver TG concentrations.
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Cheng, Shu-Chun, and 鄭淑君. "Effects of Basswood Culture Antrodia cinnamomea Ethanol Extracts on Reproductive Function in STZ-induced Diabetic Male Rats." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/51760592652480367737.
Full textAbstract:
碩士國立臺灣海洋大學
食品科學系
102
Diabetes mellitus with the subsequent generation of reactive oxygen species can affect spermatogenesis, which in turn cause male infertility. Reproductive dysfunction is a consequence of diabetes, but the underlying mechanisms are poorly understood. Antrodia cinnamomea (A. cinnamomea) is an unique mushroom in Taiwan which is commonly used for treatment of several types of cancers and inflammatory disorders. The aim of this study were to investigate the capability of anti-oxidative for A. cinnamomea ethanol extract (ACEE), and to evaluate the ameliorating effect on male reproduction in diabetic rats. Male Sprague-Dawley rats were induced into diabetes by streptozotocin (65 mg/kg) and nicotinamide (230 mg/kg) A. cinnamomea ethanol extract was tested in three doses (385, 770, 1540 mg/kg per day, p.o. daily) for 5 weeks. The results indicated that ACEE can improve the STZ-NA induced hyperglycemia and insulin resistance. It also decreased oxidative level by improving the activity of anti-oxidative enzyme. In addition, ACEE could also reduce the percentage of abnormal structure of the seminiferous tubules and sperm parameters. After the dose of 770 mg/kg ACEE administration, sperm count and motility were increased, whereas epididymal sperm abnormalities were decreased. In addition, ACEE can decrease the level of ROS on sperm. Our study indicated that A. cinnamomea have antioxidation properties to prevent diabetes-induced male reproductive damage from oxidative stress. Keywords: diabetes, male fertility, spermatogenesis, oxidative stress, Antrodia cinnamomea
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Chen, Hong-Si, and 陳虹禧. "Effect of green tea extract rich in epigallocatechin gallate on thrombogenic risk in STZ-induced diabetic rats." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/09360648479665019049.
Full textAbstract:
碩士靜宜大學
食品營養研究所
98
Hyperglycemia-induced oxidative damage plays an important role in the development of cardiovascular diseases in type 2 diabetic patients. The antioxidative, hypoglycemic, and hypolipidemic effects of green tea extract (GTE) rich in epigallocatechin gallate (EGCG) have been well-known. The purpose of this study was to investigate the effect of EGCG-rich GTE on thrombogenic risk in STZ-induced diabetic rats. Seventy-five male Wistar rats were randomly assigned into five experimental groups, including normal control(C), diabetic control(D), diabetic rats with 100mg/kg B.W./day of GTE (L), diabetic rats with 250mg/kg B.W./day of GTE (M), and diabetic rats with 500mg/kg B.W./day of GTE (H). After 8 weeks of feeding, concentration of fasting glucose, antioxidative markers (TAS、Catalase、SOD、GPx、MDA), inflammatory markers (CRP、IL-6、TNF-α), coagulation factor VII (FVII), anticoagulation factor (AT-III and Protein C), plasminogen activator inhibitor-1 (PAI-1) and von Willebrand factor (vWF) were measured. The results of this study showed that TAS was increased and MDA was decreased significantly (p<0.05) in diabetic rats fed with GTE. The concentration of fasting glucose was decreased and the activity of renal GPx was increased significantly (p<0.05) in diabetic rats with medium and high doses of GTE. When compared to diabetic control rats, the concentration of IL-6 was decreased significantly (p<0.05) in diabetic rats with high dose of GTE, however, CRP value was not affected by GTE feeding. Activity of factor VII, PAI-1 and concentration of vWF all tended to be decreased by GTE supplementation. In conclusion, supplementing EGCG-rich GTE may have beneficial effects to decrease oxidative stress, to ameliorate chronic inflammation and abnormal blood coagulation, and hence decrease the risk to develop thrombogenic complications in STZ-induced diabetic rats.
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Hoang, Lai Ming, and 賴明宏. "Effect of Dietary Rice Bran or Trivalent Chromium on Carbohydrate and Lipid Metabolism in Type 2 Diabetic Patients or STZ-Induced Diabetic Rats." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/25331539649543920394.
Full textAbstract:
博士台北醫學院
藥學研究所
90
Abstract To investigate the dietary factors affecting blood glucose and lipid parameters in type 2 diabetes patients, the research was divided into three parts. In the first part, we investigated the dietary pattern of type 2 diabetes. In the second part, we observed the effects of a fiber-rich rice bran diet on carbohydrate and lipid metabolism in STZ-induced diabetic rats and normal subjects. In the third part, we observed the effects of trivalent chromium on carbohydrate and lipid metabolism in patients with type 2 diabetes. First, we investigated the dietary pattern of type 2 diabetes. We investigated the relationship between dietary intake in the usual and blood glucose and lipid concentrations and intake over the Chinese New Year period in normal, mildly hyperglycemic, and severely hyperglycemic subjects. Samples included 22 normal subjects and 62 patients with type 2 diabetes in February and June 2000, respectively. This approach was proceeded by a 24-h dietary recall for collecting subjects’ dietary data, measuring body height and weight and determining fasting plasma glucose, hemoglobin A1c, insulin, cholesterol and triglyceride concentrations. The results show that dietary intake in the usual followed the recommendations of the Department of Health (DOH), but that dietary fiber intake was lower than that recommended by the DOH. Over the Chinese New year holiday, dietary intake was a significant increase in total caloric intake, protein and fat. This change in dietary intake increased diabetic patients’ plasma triglyceride levels. As to food choice and intake by common people, we investigated the dietary fiber content of normal diets designed by a dietitian to meet the recommendations of DOH. We asked that the dietitian design diets which contained 2000 kcal for 7 days, then used a food database for calculation of macronutrients and dietary fiber content. The results showed that the dietary fiber content of the normal diet designed by a dietitian were lower than the recommendation of DOH. Thus, increasing the dietary fiber content in the diets is a big concern. The dietary guidelines for American in 2000 indicate that increasing the whole-grain intake might increase the cereal fiber intake, which would be good for type 2 diabetics. In the second part, we observed the effects of fiber-rich rice bran diets on carbohydrate and lipid metabolism in STZ-induced diabetic rats and normal subjects. A 2 x 2 factorial experimental design was employed to evaluate the effect of a fiber- rich rice bran diet on blood glucose and lipid, and diabetic nephropathy in streptozotocin (STZ)-induced diabetic rats. Forty male adult Wistar rats were randomly divided into four groups, two of which were injected with nicotinamide and STZ to induce diabetes. Both normal and STZ-diabetic rats were fed AIN-76 diets and rice bran diets (20 g/-100 g of diet). After 4 weeks of feeding, a significantly (p < 0.05) change of STZ-diabetic rats both with and without rice bran were hyperglycemic. Blood fructosamine was ameliorated in rice bran diet-fed STZ-diabetic rats, compared with the baseline. STZ-diabetic rats fed a rice bran diet had a significantly lower blood glucose response than did those fed the diet without rice bran. These results suggest that a rice bran diet is effective in lowering blood fructosamine and improving the glucose response, but it cannot retard the development of diabetic nephropathy in STZ-diabetic rats. We further studied the effects of dietary supplementation of rice bran on postpranidal glucose and insulin responses of normal subjects. And rice bran added 30g to the diet might lower 64% the postpranidal glucose response area and lower 38% the postpranidal insulin response of normal subjects. In addition, macronutrients and dietary fiber of the diet might influence carbohydrate and lipid metabolism. Trivalent chromium supplementation in humans has been reported to improve glucose metabolism and serum lipid parameters. In the third part, we investigated the effects of trivalent chromium supplementation on carbohydrate and lipid metabolism in patients with type 2 diabetes. While there is a lower level in biological samples, very few reports have studied chromium concentrations in humans. Thus, we investigated the concentrations of total trivalent chromium in human bodies. The concentrations of total trivalent chromium in human blood and urine were determined by electrothermal atomic absorption spectrometry. We used procedure from the manufacturer and from articles in the literature, and achieved good accuracy and precision. We also analyzed normal diets containing 2000 kcal designed by a dietitian and found that there was an average of 48 g trivalent chromium in these diets. The concentration was about the same 50 g, which was estimated to be a safe and adequate daily dietary intake in 1989, but higher than 20~35 g, which was estimated to be a safe and adequate daily dietary intake in 2000. To investigate the status of young and elderly people, trivalent chromium in the blood and urine were analyzed. The results showed that blood chromium was lower 40.1% in the elderly people (40~60 years-old) than the young people (20~30 years-old). To investigate correlations between blood trivalent chromium level and blood glucose and lipid concentrations in type 2 diabetes. The results suggested that the blood trivalent chromium concentration decreased with increasing blood glucose concentration and serum lipids in diabetes and correlation coefficients were —0.99 and —0.98. A 2 x 2 factorial experimental design was employed to evaluate the effect of a chromium yeast diet on blood glucose and lipid, and diabetic nephropathy in streptozotocin (STZ)-induced diabetic rats. Forty male adult Wistar rats were randomly divided into four groups, two of which were injected with nicotinamide and STZ to induce diabetes. Both normal and STZ-diabetic rats were fed AIN-76 diets and chromium yeast diets (trivalent chromium 200 g/day by gavage). After 4 weeks of feeding, a significantly (p < 0.05) change of STZ-diabetic rats both with and without chromium yeast were hyperglycemic. But no significant changes in glucose metabolism and lipid parameters were observed. It cannot retard the development of diabetic nephropathy in STZ-diabetic rats. We investigated the effect of dietary chromium supplementation on glucose and lipid metabolism in patients with type 2 diabetes. After 3 months of supplementation, fasting blood glucose decreased significantly in severe type 2 diabetes. Because insulin sensitivity was lower in severe type 2 diabetes, chromium supplementation might increase insulin sensitivity and improve glucose metabolism. But no significant changes in lipid parameters were observed. In conclusion, rice bran supplementation might improve the glucose response in STZ-diabetic rats and normal subjects. It may prevent and improve the diabetes. Chromium supplementation might increase insulin sensitivity and improve glucose metabolism in severe type 2 diabetes.
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Ya-Ping and 譚雅萍. "Effects of garlic oil on oxidative stress and insulin secretory function in pancreas of STZ-induced diabetic rats." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/63125634881376906593.
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CHEN, TSUNG-SHENG, and 陳宗聖. "Effects of guava (Psidium guajava Linn.) juice on oxidation, inflammation and kidney injury in STZ-induced diabetic rats." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/69834189870697279960.
Full textAbstract:
碩士亞洲大學
保健營養生技學系碩士班
99
Epidemiological data indicate that diabetes is one of the major contributing factors to the high incidence and prevalence rates of dialysis in Taiwan. Approximately fifty percent of dialysis patients were originated from diabetes. Guava is one of the popular and widely processed fruits in Taiwan. In folk medicine, the roots, bark, leaves, and fruits of Guava are used to treat diarrhea, hyperglycemia, and a number of other conditions. This study thus was designed to explore the potential protective effects of fresh guava juice on oxidation, inflammation, and kidney injury in STZ-induced diabetic rats. Male Sprague-Dawley rats, based on their initial body weights, were randomly divided into four groups: control, streptozotocin (STZ), STZ plus low concentration guava juice (STZGL), and STZ plus high concentration guava juice (STZGH). Diabetic state was induced by intraperitoneal injection of 45 mg/kg body weight STZ. After induction of diabetes, the animals were treated with or without fresh guava juice for 12 weeks. Analytical results showed that fresh guava juice displayed good antioxidant properties. It could not reduce the body weights and blood sugar levels of diabetic rats. However, the kidney malondialdehyde, urine creatinine and protein levels, and erythrocyte aldose reductase activity were significantly lower (p<0.05) in the STZGH group as compared to the STZ group. The activities of kidney superoxide dismutase and catalase and the level of erythrocyte glutathione were significantly higher (p<0.05) in the STZGH group than in the STZ group. Moreover, when compared with the STZ group, serum triglycerol, nitric oxide, interleukin-6 levels of the STZGH group decreased 27.7%, 36.0%, and 12.8%, respectively, while serum interleukin-10 level and renal glutathione peroxidase activity raised 22.5% and 28.5%, respectively. The Western blot results revealed that fresh guava juice significantly suppressed (p<0.05) transforming growth factor-β1 protein expression in the kidney, but it had no effect on the protein expression of protein kinase C-. Histological examination showed that fresh guava juice obviously reduced renal injury, such as glomerular basement membrane thickening and mesangial matrix expansion. The findings of present study implied that fresh guava juice could not improve hyperglycemia in STZ-induced diabetic rats, but its potential antioxidantive and anti-inflammatory capacities could ameliorate kidney injury.
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Chiang, Yi-Hsuan, and 江怡萱. "Effect of Dehulled Adlay Added to Mung Bean on Lipid and Carbohydrate Metabolism in STZ-induced Diabetic Rats." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/57108476453359182254.
Full textAbstract:
碩士國立海洋大學
食品科學系
89
Abstract The aim of this study was to investigate the effect dehulled adlay and mung bean in streptozocin (STZ) — induced diabetic rats. In experiment I was to evaluate the dehulled adlay starch and mung bean starch, characterized by different glycemic indices, on glucose and insulin response in normal and diabetic rats. The α- amylase digestibility was higher for dehulled adlay starch than that of the corn starch and mung bean starch. The glycemic index of the dehulled adlay starch was higher than that of the corn starch and mung bean starch when measured in vivo in three group of normal rats. In a subsequent study, normal and diabetic male Wistar rats was assessed by oral glucose tolerance test (OGTT) and added to mung bean extract. We found serum glucose responses were significantly lower in rats received mung besn extract in either normal or diabetic rats. In experiment II was to compare the effects of dehulled adlay added to mung bean on lipid and carbohydrate metabolism in diabetic rats. Male Wistar rats were used as experiment animal model and were made diabetic status by intravenous injection of 60 mg per kg of streptozotocin. The experiment laster four weeks and divided into three groups: (1) corn starch (2) dehulled adlay (3) dehulled adlay+ mung bean. The results showed that diabetic rats fed dehuller adlay groups had significantly heavier body weight, adipose tissue weight and relative adipose weight, but when added to mung bean, the difference was not significant with corn starch group. In addition, dehulled adlay decreased plasma total cholesterol levels, LDL-C and VLDL-C in diabetic rats. Added to mung bean however, the difference was not significant compared to corn starch diet. After fed dehulled adlay added to mung bean for four weeks, had significantly risen plasma triglyceride, VLDL-TG. Although dehulled adlay diet were not influence on plasma glucose levels, and mung bean increased significantly plasma glucose and phospholipid levels. In the other hand, dehulled adlay groups had markedly decreased plasma, liver, and kidney TBARS levers, whereas added to mung bean were higher. Especially, dehulled adlay added to mung bean diet increased significantly lipid peroxidation and antioxidative enzyme, glutathione peroxiadase (GSH Px), in diabetic rats.
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Sun, Yu-Chun, and 孫郁淳. "Effects of guava fruit extracts on glucose tolerance improvement and carbohydrate metabolism in STZ-NA induced diabetic rats." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/05725137060826992272.
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Wu, Siao-Wei, and 吳曉葳. "Effect of insulin producing cells from human adipose derived stem cells transplantation on blood glucose in STZ-induced diabetic rats." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/07072067632421255841.
Full textAbstract:
碩士靜宜大學
食品營養學系
100
Diabetes mellitus (DM) was the 5th of top 10 causes of death in Taiwan and high prevalence in recent years. Therefore, study of its treatment is an important issue. In recent research stem cells is used to rebuild injured tissue or treatment of diseases has been considerable progress. However stem cells are difficult to obtain. The purpose of this study was to observe the effect of transplanted insulin producing cells (IPCs) differentiation from human adipose derived stem cells (ADSCs) by medical operation on blood glucose in streptozotocin (STZ) induced diabetic rats. Experiments used single-dose 65 mg/Kg BW STZ (DM) or combined 120 mg/Kg BW nicotinamide (DM (120N)) injection to induce different level hyperglycemia of diabetes, and ADSCs or IPCs transplanted into renal capsule of diabetic rats. Rats were divided to three groups of DM +ADSCs, DM +IPCs and DM (120N) +IPCs. Experiment observed for eight weeks. Results showed that causal blood glucose was significantly reduced and returned to normal, glucose tolerance was significantly improved, the urine glucose, water and food intake were improved in DM (120N) +IPCs group. No significantly improved in any items were observed in the other two groups. Therefore, IPCs differentiated from ADSCs improved hyperglycemia in mild diabetic rats. The differentiation IPCs from adipose cell by medical operation may be used for diabetes treatment in future.
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Chien-Hui and 柯千惠. "A study on the mechanism of anti-diabetic effects of leaf essential oil of Cinnamomum osmophloeum Kaneh. by using STZ-induced diabetic rats and C2C12 cell line." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/16016284433712668991.
Full textAbstract:
碩士中山醫學大學
營養學研究所
100
According to previous study, cinnamon extract possesses an anti-diabetic effect. Our previous study have found the leave essential oil of Cinnamomum osmophloeum Kaneh.(TC) improves oral-glucose tolerance and insulin tolerance in diabetic rats and it is associated with both anti-oxidant and anti-inflammatory effects. The aim of the present study is to investigate the mechanism underlying which TC affects blood glucose control in diabetes with in vivo and in vitro studies. Male Wistar rats were induced to be diabetes with Streptozotocin (STZ, 65mg/kg BW) and received every other day for eight times by gavage TC (13、26 or 54mg/kg BW), cinnamaldehyde (CA, 40 mg/ kg BW), glibenclamide (GC, 1.2mg/kg BW) or vehicle (corn oil, 2ml/kg BW). In vitro, C2C12 myotubes were induced to differentiate for 6 days, followed by pre-treatment with TC (0.2 μg/ml、2 μg/ml、20 μg/ml) for 2 hours. DMSO (concentration in culture???) was used as vehicle and linalool (0.8 μg/ml) was used as positive control. In vivo, it showed that TC restrained DM progressing, and was more effective than CA and GC. At the dose 26 mg/kg, TC increased plasma insulin concentration to a level similar to that of the control group, and was more effective than that of CA and GC. It does not seem that the ameliorated peripheral insulin level was via the regulation on PDX-1 expression. Diabetes suppressed the expression of IR-beta, GLUT4, phosphorylation of Akt-ser and Akt-thr, and the activity of PI3K, while induced the expression of SOCS3 in skeletal muscle and adipose tissue.In skeletal muscle tissues, it showed that 13 mg/kg of TC up-regulated the expression of GLUT4, increased phosporylation of Akt-ser and Akt-thr, 26 mg/kg of TC increased the activity of PI3K, and all doses of TC except the lowest dose, CA and GC suppressed diabetes-induced elevation of SOCS3 expression. In adipose tissue, all treatment promoted the expression of IR-beta and13 mg/kg of TC increased the expression of GLUT4, the activity of PI3K, and lowered expression of SOCS3 in diabetic rats. In vitro, the expression of IR-beta, GLUT4, phosporylation of Akt, activity of PI3K, and glucose uptake rate showed not affected by the differentiated C2C12 muscle cell line in the absence of insulin. However, 0.2 μg/ml of TC promoted insulin-induced expression of IR-beta and activity of PI3K to a higher extent than that of linalool. Similarly, 2 μg/ml of TC promoted insulin-induced phosphorylation of Akt and glucose uptake rate to a higher extent than that of linalool. Pretreat the cells with 0.2 μg/ml of TC and with linalool down-regulated the expression of SOCS3. In summary, the present study demonstrated that TC improved insulin sensitivity in vivo and in vitro under the appropriate dose, and its effects is associated with improved insulin signaling protein expression and activation.
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Lee, Kao-Ting, and 李高廷. "Effects of oriental plums on the expressions of the cognitive function- and the cerebral neurodegeneration-related proteins in STZ-induced diabetic rats." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/10266019575968199700.
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Chen, po-Han, and 陳柏翰. "Comparison between the therapeutic effect of differentiated and undifferentiated human umbilical cord Wharton's jelly mesenchymal stem cells in STZ-induced diabetic rats." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/vu8z5m.
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Yu-Hsin and 陳宇欣. "The effect of liquid extracts and polyphenol extracts from Hibiscus Sabdariffa Linnaeus on nephropathy and lipid metabolism in STZ-induced diabetic rats." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/90456763096390499662.
Full textAbstract:
碩士中山醫學大學
生化暨生物科技研究所
94
The major mortality of diabetes mellitus (DM) was usually caused of the complications, such as the cardiovascular diseases and renal failure. This result could be concluded that under the circumstance of high blood sugar for a long time, abundant free radicals could be produced in diabetes patients; and furthermore, the ability of anti-oxidation could be reduced. The oxidative stress could cause macromolecules oxidative and further damage the organ, such as kidney and cardiovascular system. In this study, streptozotocin (STZ) was administrated to induce SD rats type I DM. After the induction completed, 100mg/kg、400mg/kg Hibiscus sabdariffa extracts (HSE) or 100mg/kg、200mg/kg Hibiscus sabdariffa polyphenols (HPE) were fed by oral tube to observe the effects on DM rats. In serum data, we did not observe the restrain result to the blood glucose. However, either HSE or HPE treated groups, the levels of serum albumin, triglyceride, total cholesterol and low density lipoprotein were improved. In TBARs assay, HSE and HPE also possessed the ability to reduce lipid peroxidation in liver. Additionally, in kidney section, we observed the hydropic change caused of the glucose, it would make the cell damage. When HSE or HPE pretreated, this phenomena was improved. Further, in kidney protein lysate, we found HSE or HPE could protect the kidney via increasing Akt expression to acivate Bad and 14-3-3γ expressions, and regulate the transcription factor NF-κB to promote kidney cell survive. We assumed that via reducing oxidative stress, HSE and HPE possessed the ability to improve the high glucose induced abnormal lipid metabolism and protect kidney damage.
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Hao-Yu and 張皓瑜. "The effect of leaf essential oil of Cinnamomum osmophloeum Kaneh on the expression of inflammosome related protein and the development of diabetic nephropathy in STZ-diabetes rats." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/55099933986076331414.
Full textAbstract:
碩士中山醫學大學
營養學研究所
99
The aim of the present study is to investigate the effect of leave essential oil of Cinnamomun osmophloeum Kaneh. (TC) on the development of diabetic neuropathy and on the expression of NALP3 inflammasome related molecules. Male Wistar rats were induced to be diabetes with STZ (65mg/kg BW, iv). Control rats were injected with vehicle (citrate buffer, pH 4.2). At three days after injection, rats confirmed to be diabetes were gavaged vehicle (corn oil, 2 ml/kg BW), low-, middle-, or high-dose of TC (12, 26, or 54 mg/kg BW, respectively), cinnamaldehyde (C40; 40 mg/kg BW), or glibenclamide (GL; 1.2 mg/kg BW) every other day and for eight times. Control rats were gavaged corn oil (2 ml/kg BW). On day 20 after the induction of diabetes urine were collected during a 24 h period for the analysis of creatinine and albumin. Rats were killed followed by the collection of blood for the analysis of creatinine and BUN and organs and tissues were collected for Western blotting analysis on NALP3, ASC, and Caspase-1. The cryostat sections of kidney were prepared for MPO staining and cross-sections of paraffin-embedded kidney were prepared for morphological study. It was found that all tested doses of TC reversed diabetes-induced metabolic changes to an extent similar to that by C40 and GL. All tested doses of TC also significantly reversed micro-albumin urine and BUN in diabetes; middle-dose of TC significantly ameliorated CCR and GFR while high-dose of TC only ameliorated CCR. Both C40 and GL ameliorated BUN but neither of them affected CCR, GFR, or micro-albumin urine. All doses of TC, C40 and GL ameliorated phagocyte infiltration and normalized morphology of kidney in diabetes.Low-dose of TC reversed elevated ASC in lymph nodes and ileum, middle-dose of TC reversed NALP3 in liver and Caspase-1 in splenocytes, while C40 only reversed ASC in splenocytes in diabetes. In conclusion, the present study showed a prophylactic effect of TC on the development of diabetes nephropathy and lowered the expression of NALP3 inflammasome related molecules induced by diabetes and such effects of TC is greater than cinnamaldehyde. The functional composition in TC remained to be clarified in the future.
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Chiang, Ching-Jung, and 蔣青蓉. "Study of vitamin B6 in conjunction with insulin treatment of STZ-induced diabetic rats on the platelet aggregation and the vascular and endothelium." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/02495288788924080717.
Full textAbstract:
碩士國立成功大學
生物學系
84
The vascular complication has been considered to be one of the major risk factors of mortality in diabetes mellitus. The principal cause of this vascular disease is atherosclerosis. Endothelial injury has been suggested as a potential initiating event in the development of atherosclerosis. It has also been postulated that thrombogenic factors, such as platelet hyperactivity or platelet hyperaggregation, may be an early pathological process in atherosclerosis. Vitamin B6 has been found to contribute to protect the vascular endothelial injury from platelet activation; inhibit the platelet aggregation and blood clotting; inhibit thromboxane A2 production of platelet; increase prostaglandin I2 production of the vascular endothelial cells and affect the morphology of vascular endothelial cells. It is possible that vitamin B6 may exert certain antithrombotic and antiatherogenic effects. The purpose of this study was to investigate whether vitamin B6 alleviates the vascular complications of insulin-treated streptozotocin (STZ) -induced diabetic rats. Diabetic animals were treated with or without vitamin B6 and / or insulin. Platelet aggregation induced by ADP (10μM) or Thrombin (0.05 U/mL) was measured in plasma rich platelet of normal and diabetic animals. 14C-thromboxane B2 (14C-TxB2) production of platelet , using 14C-arachidonic acid ( 14C-AA) as a precursor, was assayed by means of radiochromatography scanning and autoradiography. 14C-TxB2 was quantified by scintillation counter. The results showed that vitamin B6 in conjunction with insulin treatment revealed lower blood glucose than either vitamin B6 or insulin treatment alone. Similarly, platelet aggregation induced by ADP or Thrombin and TxB2 production of diabetes with vitamin B6 and insulin treatment were significantly decreased. Blood glucose concentrations were positively correlated to the platelet aggregation or thromboxane B2 production. Scanning electric microscopy showed a decreased platelets and blood cells adhesion to the vascular wall. These data indicated that vitamin B6 in conjunction with insulin treatment seemed to be better than vitamin B6 or insulin treatment alone in controlling blood glucose, inhibiting platelet aggregation, decreasing TxB2 production and preventing platelet and blood cells adhesion to the vascular wall. In summary, vitamin B6 in conjunction with insulin treatment of diabetes mellitus might provide a possible role in preventing or reducing the progress of the vascular complications in later stage of diabetes mellitus.
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Cheng, Yung-Yuan, and 鄭詠元. "Comparison of human Wharton\'s jelly mesenchymal stem cells in normoxia culture and hypoxia culture for different treatment of STZ-induced diabetic rats." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/4mk22t.
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Chen, Chia-Wen, and 陳嘉文. "The Effects of various rice containing different amounts of amylose on lipid metabolism in STZ-induced diabetic rats in low dietary fiber level status." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/13546730057860706525.
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碩士台北醫學院
保健營養學研究所
90
The purpose of this study was to investigate the effects of various rice containing different amounts of amylose on lipid metabolism in STZ-induced diabetic rats given a low-dietary-fiber diet. This study was divided into two experiments. Both experiments used 16-week old male Wistar normal or diabetic rats as experimental animals for 4 weeks of experimental dietary feeding. Diabetes was induced by nicotinamide and streptozotocin injection. The formulas of these experimental diets were modified from the AIN93M formula. In experiment I, the experimental rats were divided into two groups: a normal rat group (NC) and a diabetic rat group (DC), each group with ten rats. Both groups were fed the experimental diets with 5% dietary fiber and gelatinized cornstarch as the carbohydrate source. The purpose of experiment I was to investigate differences in lipid metabolism between normal rats and diabetic rats. In experiment II, the rats were divided into three groups and fed three diets Indica rice (DI), Japonica rice (DJ), and Waxy rice (DW), each group with ten diabetic rats. All groups were fed the experimental diets with 2.5% dietary fiber and gelatinized Indica rice, Japonica rice or waxy rice as the carbohydrate source. The purpose of experiment II was to investigate differences in lipid metabolism in diabetic rats fed the three diets with 2.5% dietary fiber. Fasting blood sampling and an intraperitoneal glucose intolerance test (IPGTT) were conducted in the first week initially and the fourth week in both experiments. At the end of both experiments, all rats were sacrificed, and their livers and epididymal fat pads were collected. Results of experiment I showed those diabetic rats did not have hypercholestinemia or hypertriglyceridemia, and the normal rats had significantly higher activity of fatty acid synthase in the liver than did diabetic rats. Results of experiment II showed that rats eating the DI and DJ diets had significantly higher plasma TG and VLDL-TG concentrations, than did those on the DW diet. Fatty acid synthase activities of the liver in the DI and DJ diets were significant higher than that of the DW diet. But rats on the DW diet had significantly higher activity of fatty acid synthase in epididymal fat pads. In conclusion, hypertriglyceridemia was induced in diabetic rats by feeding an experimental diet with 2.5% dietary fiber and higher amylose rice as the carbohydrate source.
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Ansell, David M., C. Marsh, L. Walker, M. J. Hardman, and K. Holden. "Evaluating STZ-Induced Impaired Wound Healing in Rats." 2017. http://hdl.handle.net/10454/17773.
Full text
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Lin, Ting-Wen, and 林亭妏. "The mechanism of insulin-improved fatty liver in STZ-induced diabetes male rats." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/q2b72k.
Full textAbstract:
碩士臺北市立大學
運動科學研究所
106
Purpose: (1) Observe whether insulin could improve diabetes in liver, and oxidative stress or antioxidant capacity level after acute exercise challenges. (2) Observe whether the insulin treatment had equal efficacy in the old rats. Method: There were two experiment in this study. Study one: 18 weeks old rats injected STZ to induce diabetes, insulin treatment, and down heel running at the last of the experiment. Rats were divided to control group (C), diabetes group (S), diabetes plus insulin (SI), control with down heel running (EC), diabetes with down heel running (ES), diabetes plus insulin with down heel running (ESI). Study two: 22 months old rats injected STZ to induce diabetes, and insulin treatment. Rats were divided to control group (OC), diabetes group (OS), diabetes plus insulin (OSI). After one week acclimation, rats were individually injected with sodium citrate (also referred to as STZ carrier) or STZ, and fasting blood glucose was measured with a blood glucose meter. When the blood glucose level was 300 mg/ dL or more, they were injected twice daily saline or insulin (0.25 IU/ml). Feeding a total of 14 days, pentobarbital (40 mg/kg) was anesthetized before injection of STZ and at the end of the feeding period. Testing body composition by DEXA, then sacrifice and take the liver and perirenal fat to assay for the production of TBARS and Glutathione. Hematoxylin and eosin stain will be used to determine liver steatosis and size of perirenal fat cell, and Masson's staining will be used to determine fibrosis. Data were analyzed statistically by two-way ANOVA. Post-hoc were performed one-way ANOVA and Bonferroni multiple tests to differentiate the effect of S and SI group. P<0.05 were considered as significant difference. Independent-sample T test was used to differentiate the effect of young and old/ young and exercise groups. P<0.05 were considered as significant difference. Result: Aging and diabetes would cause weight loss. Diabetes in the elderly would cause a rapid decline in lean mass, insulin treatment would slow down the loss of fat after STZ injection. Histological in liver produced more vacuolation in elderly diabetes. After an acute exercise challenge in young rats, the mechanism of antioxidant capacity of glutathione was upregulated. Both insulin treatment and acute exercise challenge can increase antioxidant capacity and decrease oxidative stress. Conclusion: Insulin treatment was effective in diabetes weight loss, acute exercise challenge contributed to increase antioxidant capacity, also with insulin treatment can decrease oxidative stress.