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1
Woods, Caroline M. "The fungal ecology of Sitka spruce stumps." Thesis, University of Aberdeen, 1996. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU083296.
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A study of the fungal ecology of Picea sitchensis stumps on mineral soils on first rotation sites in Scotland was carried out to determine fungal colonization, succession and the mechanisms of fungal interaction. Fungal and bacterial colonization of stump and buttress roots of stumps 0, 7, 28 days, 12, 16 and 48/53 months old was assessed. Melanotus proteus was found in all 12 month old stumps; Sistotrema brinkmanni was recorded most frequently in 16 and 48/53 month old stumps. A series of in vitro experiments was carried out to identify interactions occurring between pairs of P. sitchensis fungi on Norkrans agar, P. sitchensis sawdust, root blocks and billets, to determine possible modes of interaction occurring in vivo. Fungi exhibiting antagonism toward Heterobasidion annosum in vitro were noted to determine possible in vivo applications as curative/preventative biological controls against H. annosum. Sitka spruce stumps were highly receptive to H. annosum basidiospore infection up to 24 hours after felling and showed a significant level of receptivity 7 days after felling. M. proteus infection was lower in live stumps, compared to dead or moribund stumps, and was reduced or inhibited in stumps inoculated with Resinicium bicolor sawdust inoculum. In vitro experiments indicated that 5% urea prevented M. proteus basidiospore germination and hyphal growth. Treating stumps or billets with a 20% urea solution, however, had no significant effect on M. proteus colonization. Antifungal metabolites were detectable in 85% of the 25 fungal species tested representing members of the Basidiomycotina, Deuteromycotina and Ascomycotina, when bioassayed with Cladosporium cucumerinum. The production of antifungal metabolites in Sitka spruce stumps by H. annosum, R. bicolor, Stereum sanguinolentum, M. proteus and Hypholoma fasciculare was demonstrated.
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Murray, Alison Catherine. "The bacterial ecology of Sitka spruce stumps." Thesis, University of Aberdeen, 1998. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU106025.
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The distribution of bacteria in Sitka spruce stumps between one and ten years old was investigated by dilution plating wood chips taken from seven sampling positions up to 55 mm from the stump surface. The same wood chips were used to determine the presence Basidiomycotina within the stump wood. The number of bacterial colony forming units in the wood samples decreased in stumps between one and five years old, reaching a minimum after six years, before increasing in stumps up to ten years old. There was also a decline in numbers of bacteria isolated with increasing distance from the stump surface and the stump edge. A similar trend was detected in the diversity of the bacterial community. The size of the bacterial population was also correlated with stump moisture content. The presence of Basidiomycotina was associated with the presence of bacteria within the sample. However, the number of bacteria isolated from samples containing Basidiomycotina did not differ from that where no Basidiomycotina were isolated. Interactions between wood-decay fungi, including Heterobasidion annosum, and bacterial isolates were studied in vitro. The growth medium, timing of inoculations, and bacterial and fungal species tested were fund to significantly affect the outcome of the interaction. Bacterial isolates degraded cellulose, pectin and starch, cellulolytic ability increased with increasing stump age. Siderophores and chitinase, potential antifungal compounds, were produced by 29% and 2% of isolates respectively, however, these isolates had no effect on the distribution of Basidiomycotina in the stumps. Four groups of bacteria were identified from cluster analysis of 13 phenotypic characteristics. Different groups of bacteria were found to dominate bacteria isolated from stumps of different ages indicating that bacterial successions occur in decaying Sitka spruce stumps.
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Casselli, Vinicius. "Remoção de tocos de eucalipto com sistema de serra tubular." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/11/11150/tde-21012013-092719/.
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Os tocos, remanescentes da colheita florestal, causam um impedimento físico às operações com máquinas para o preparo do solo para um próximo plantio, ou mesmo quando a área onde estava sendo cultivado o eucalipto vai ser usada para o plantio de uma nova cultura ou estabelecer pastagem para pecuária. O objetivo deste trabalho foi estudar e avaliar o desempenho de uma serra tubular para a extração de tocos de eucalipto remanescentes da colheita. Este implemento, montado em uma grua hidráulica acoplada a um trator agrícola, é constituído de um tubo metálico com borda serrilhada, com diâmetro de 65 cm e altura interna de 70 cm, realizando o corte do sistema radicular e a extração apenas do toco e raízes próximas. O processo de extração com serra tubular foi comparado à extração de tocos com uma escavadora hidráulica equipada com destocador. Para a comparação entre o desempenho da serra tubular com a escavadora, foram realizadas extrações em quatro parcelas, com 108 tocos cada, sendo duas parcelas para cada sistema. Foram mensurados o tempo de extração para cada toco e o tempo de deslocamento das máquinas em cada parcela. O processo de extração pela serra tubular causou menos alterações na estrutura do solo, nas linhas e nas entrelinhas de plantio, se comparado ao uso da escavadora, porém o tempo de extração da serra foi superior em mais de 8 vezes ao tempo da escavadora. Foram realizadas extrações de tocos com DAB (diâmetros a altura da base) variando entre 12 e 31 cm, sendo que o tempo de extração não apresentou diferenças significativas entre os diferentes diâmetros para ambos os tratamentos. A serra tubular apresentou pouca mobilidade e pouca área de atuação em cada parada, contrariando as expectativas do projeto inicial. As análises econômicas mostraram custo operacional superior para a escavadora hidráulica, porém com maior produtividade comparado à serra tubular, resultando em menor custo de extração por unidade de toco.The stumps, remained from forest harvesting, cause a physical impediment to operations with machinery for soil preparation to the next plantation, or even when the area that had eucalyptus plantation will be used to plant a new crop or establish pasture for livestock. This paper aim to study and evaluate the performance of a tubular saw for the extraction of Eucalyptus stumps remaining after the harvesting. This accessory mounted on a hydraulic crane, attached to a farm tractor, consists of a metal tube with a serrated edge, with a diameter of 65 cm. and internal height of 70 cm., making the cut root and extract only the stump and close roots. The extraction process with tubular saw was compared with an excavator equipped with a stump remover. The extractions were performed in two plots and two parcels with 108 stumps on each for both systems. It was measured the extraction time for each stump and moving time during the extractions. The extraction process by tubular saw caused less soil disturbance on its structure on planting lines and between the planting lines, compared with the excavator use, but the extraction time with the tubular saw was more than five times compared to the excavators extraction. The extractions were performed on stumps with different DAB (diameter at base height), ranging between 12 to 31 cm, and the extraction time showed no significant differences between the different diameters for both treatments. The tubular saw had little mobility and low operating area at each stop, contrary to the expectations of the initial Project. Economic analyzes showed higher operational cost for the hydraulic excavator, but higher productivity compared to tubular saw on a farm tractor, resulting in lower extraction cost per stump.
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Xu, Yansong W. "A hierarchical diagnostic system for STUMPS-based BIST structures." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0013/MQ60197.pdf.
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You, Sijun. "Long-term fate of non-neuronal cells in denervated nerve stumps." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1995. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq22046.pdf.
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Abrahamsson, Markus. "High-stumps and wood living beetles in the Swedish production forest landscape /." Alnarp : Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/2007126.pdf.
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Rizk, Charbel. "Ondskans Problem : En granskning av Richard Swinburnes teodicé i ljuset av Eleonore Stumps tankegångar." Thesis, Södertörns högskola, Institutionen för kultur och kommunikation, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-6199.
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Ondskans problem är ett gammalt (religions)filosofiskt problem som, i grova drag, frågar om existensen av en allsmäktig, allvetande och allgod Gud är förenlig med ondskan i världen, samt varför en sådan Gud tillåter ondska. Filosofer (som svarar jakande på frågan om) har utvecklat ett svar på frågan varför Gud tillåter ondska. Syftet med denna uppsats är följaktligen att redogöra för Richard Swinburnes svar på frågan varför Gud tillåter ondska, samt granska svaret i ljuset av Eleonore Stumps tankegångar.
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Rönnberg, Jonas. "Incidence of root and butt rot in consecutive rotations, with emphasis on Heterobasidion annosum in Norway spruce /." Alnarp : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 1999. http://epsilon.slu.se/avh/1999/91-576-5630-4.pdf.
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Caruso, Alexandro. "Lichen diversity on stems, slash, and stumps in managed boreal forests : impact of whole-tree harvest /." Uppsala : Swedish University of Agricultural Sciences, 2008. http://diss-epsilon.slu.se/archive/00001675/.
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Thesis (doctoral)--Swedish University of Agricultural Sciences, 2008.Includes reprints of four papers and manuscripts co-authored with others. Includes bibliographical references. Also partially available electronically via World Wide Web in PDF format; online version lacks reprints of four papers and manuscripts.
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Lopes, Cátia Sofia dos Santos. "Avaliação das características dos cepos e raízes de Eucalypto como matéria-prima papeleira." Master's thesis, ISA/UTL, 2012. http://hdl.handle.net/10400.5/5361.
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Mestrado em Engenharia Florestal e dos Recursos Naturais - Instituto Superior de AgronomiaIn this study we used stumps and roots of Eucalyptus globulus Labill., from six places, to evaluated its quality as raw-material for papermaking, using two chemical processes: Kraft and ASAM. It was also evaluated the introduction of two pre-treatments: a) extraction with ethanol and water, b) water to promote auto-hydrolysis. It was determined yields of the pulps were the material without pre-treatment presented 42.7% (Kraft) and 49.3% (ASAM); extracted wood 47.5% (Kraft) and 51.7% (ASAM); auto-hydrolyzed material 38.4% (Kraft) and 42.5% (ASAM). It also determined the Kappa number, the degree of polymerization and brightness. Pulps without pre-treatment presented values of (ASAM and Kraft, respectively): 14 K and 36 K, 941 DP0,85 and 929 DP0,85; 25.9% and 23.6% of brightness. For pulps obtained with extracted wood: 11 K and 18 K; 845 DP0,85 and 1004 DP0,85; 30.8% and 35.8% of brightness. For pulps from auto-hydrolyzed materials: 14 K and 20 K; 552 DP0,85 and 574 DP0,85; 27.1% and 28.7% of brightness. There has also been done biometric studies and was obtained the following average results: average length 0.9 mm, a fiber width of 21.4 μm and lumen widths of 9.0 μm. It was determined the index evaluation of paper, based on biometric parameters: number of Runkel (1.4), felting coefficient (43.7) and soupless coefficient (42.5%).
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Sucre, Eric Brandon. "Soil resource heterogeneity and site quality in Southern Appalachian hardwood forests: Impact of decomposing stumps, geology and salamander abundance." Diss., Virginia Tech, 2008. http://hdl.handle.net/10919/29470.
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The Southern Appalachian hardwood forests contain a wide diversity of flora and fauna. Understanding processes that affect nutrient availability in these forests is essential for sound forest management. Three interconnected research projects regarding soil resource heterogeneity were designed to increase our understanding of this ecosystem. The objective of these projects were as follows: 1) to examine and quantify the role of decaying stumps in regards to total carbon (C) and nitrogen (N) pools and fine-root dynamics, 2) compare and contrast the use of ground-penetrating radar (GPR) vs. a soil auger for estimating soil depth and site quality and 3) to evaluate how eastern red-backed salamanders (Plethodon cinereus) affect N-availability.
For the stump study, results show that decomposing stumps occupy approximately 1.2% of the total soil volume and constitute 4% and 10% of total soil N and C pools. Significant differences in N (p = 0.0114), C (p = 0.0172), microbial biomass C (p = 0.0004), potentially mineralizable N (p = 0.0042), and extractable NH4+ (p = 0.0312) concentrations were observed when compared to mineral soil horizons. In particular, potentially mineralizable N was 2.5 times greater in stump soil than the A-horizon (103 vs. 39 mg kg-1), 2.7 times greater for extractable NH4+ (16 vs. 6 mg kg-1) and almost 4 times greater for MBC (1528 vs. 397 mg kg-1). These measured properties suggest higher N-availability, organic matter turnover and N uptake in stump soil versus the bulk soil. 19% of the total fine root length and 14% of fine root surface area also occurred in the stump soil. The increased fine root length suggests higher concentrations of labile nutrient in the stumps since roots often proliferate in areas with higher nutrient availability. Significant differences occurred in N and C concentrations between all four decay classes and the A-horizon, which validated the use of this system and the need to calculate weighted averages based on the frequency and soil volume influenced by each decay class.
In the GPR Study, depth estimations were shallower using a soil auger compared to estimates obtained using GPR across all plots (p = 0.0002; Figure 3.4). On a soil volume basis, this was equivalent to about 3500 m3 of soil per hectare unaccounted for using traditional methods. In regards to using soil depth as a predictor for site quality, no significant relationships were observed with soil depth estimations obtained from the auger (Table 3.3). On the other hand, depth measurements from GPR explained significant amounts of variation across all sites and by physiographic region. Across all sites, soil depth estimates from GPR explained 45.5% of the residual variation (p = 0.001; Table 3.3). When the data were stratified by physiographic region, a higher amount of variation was explained by the regression equations; 85% for the Cumberland Plateau (p = 0.009), 86.7% for the Allegheny Plateau (0.007) and 66.7% for the Ridge and Valley (p = 0.013), respectively (Table 4.2). Results from this study demonstrate how inaccurate current methods can be for estimating soil depth rocky forests soils. Furthermore, depth estimations from GPR can be used to increase the accuracy of site quality in the southern Appalachians.
In the salamander study, no significant salamander density treatment or treatment by time effects were observed over the entire study period (p < 0.05). However, when the data were separated by individual sampling periods a few significant treatment by time interactions occurred: 1) during August 2006 for available NH4+ under the forest floor (i.e. horizontal cation membranes; p = 0.001), 2) August and 3) September 2006 for available NH4+ in the A-horizon (p = 0.026), and 4) May 2007 for available NO3- under the forest floor (p = 0.011). As a result of these trends, an index of cumulative N-availability (i.e. NH4+ and NO3-) under the forest floor and in the A-horizon was examined through the entire study period. Cumulative N-availability under the forest floor was consistently higher in the low- and medium-density salamander treatments compared to the high-density treatment. For cumulative N-availability in the A-horizon, a gradient of high to low N-availability existed as salamander density increased. Factors such as a prolonged drought in 2007 may have affected our ability to accurately assess the effects of salamanders on N-availability. We concluded that higher salamander densities do not increase N-availability.
Implementing methodologies that accurately account for soil nutrient pools such as stump soil, physical properties such as depth and fauna such as salamanders, increase our understanding of factors that regulate site productivity in these ecosystems. As a result, landscape-level and stand-level management decisions can be conducted more effectively.Ph. D.
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Mitchell, Andrew Computer Science & Engineering Faculty of Engineering UNSW. "An approach to boosting from positive-only data." Awarded by:University of New South Wales. Computer Science and Engineering, 2004. http://handle.unsw.edu.au/1959.4/20678.
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Ensemble techniques have recently been used to enhance the performance of machine learning methods. However, current ensemble techniques for classification require both positive and negative data to produce a result that is both meaningful and useful. Negative data is, however, sometimes difficult, expensive or impossible to access. In this thesis a learning framework is described that has a very close relationship to boosting. Within this framework a method is described which bears remarkable similarities to boosting stumps and that does not rely on negative examples. This is surprising since learning from positive-only data has traditionally been difficult. An empirical methodology is described and deployed for testing positive-only learning systems using commonly available multiclass datasets to compare these learning systems with each other and with multiclass learning systems. Empirical results show that our positive-only boosting-like method learns, using stumps as a base learner and from positive data only, successfully, and in the process does not pay too heavy a price in accuracy compared to learners that have access to both positive and negative data. We also describe methods of using positive-only learners on multiclass learning tasks and vice versa and empirically demonstrate the superiority of our method of learning in a boosting-like fashion from positive-only data over a traditional multiclass learner converted to learn from positive-only data. Finally we examine some alternative frameworks, such as when additional unlabelled training examples are given. Some theoretical justifications of the results and methods are also provided.
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Siqueira, Gabriela Ferraz de [UNESP]. "Eficácia da mistura de glifosato a outros maturadores na cana-de-açúcar (Saccharum spp.)." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/86398.
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siqueira_gf_me_botfca.pdf: 855761 bytes, checksum: 59ef306636208d8e19ab66bbdaa297ed (MD5)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A maturação natural, em início de safra, pode ser deficiente, mesmo em variedades precoces e o emprego de maturadores químicos destaca-se como uma ferramenta importante para antecipar o processo de maturação, promover melhorias na qualidade da matéria-prima a ser processada, otimizar os resultados agro-industriais e econômicos e auxiliar no planejamento da safra. Dentre os produtos utilizados, o glifosato é um dos que proporciona maior eficiência na antecipação da colheita, contudo, frequentemente diminui a rebrota da soqueira reduzindo a produtividade do canavial na safra posterior. Técnicos e produtores têm misturado o glifosato a outros maturadores, sem embasamento técnico-científico, acreditando melhorar a eficiência desses e como forma de redução de custo. O projeto teve por objetivo estudar a eficácia da mistura de glifosato a sulfometuron metil ou etil-trinexapac, e as alterações fisiológicas na cana-de-açúcar bem como suas implicações na produtividade, qualidade tecnológica e na rebrota da soqueira, em aplicação em início de safra. O projeto de pesquisa foi instalado e conduzido nos anos agrícolas de 2007 e 2008 em cana soca, em área pertencente ao GRUPO COSAN, Unidade Barra, no município de Igaraçu do Tietê, Estado de São Paulo. O delineamento experimental utilizado foi em blocos casualizados com cinco repetições. O experimento foi constituído de seis tratamentos: 1 – testemunha, sem aplicação de maturador, 2 - aplicação de glifosato (produto comercial Roundup), 3 - aplicação de sulfometuron metil (produto comercial Curavial), 4 - aplicação de etil-trinexapac (produto comercial Moddus), 5 - aplicação da mistura glifosato + sulfometuron metil e 6 - aplicação da mistura glifosato + etil-trinexapac. As doses...
The natural maturation, early harvest, may be poor, even in early varieties and the use of chemical ripeners stands out as an important tool to advance the maturation process, further improvements in the quality of raw material to be processed, optimize results agro-industrial and economic and assist in the planning of the harvest. Among the products used, glyphosate is one that provides greater efficiency in anticipation of the harvest, however, often reduces the regrowth of the ratoon cane reducing the productivity of sugarcane in crop later. Technicians and producers have mixed the glyphosate with other ripeners, without basement technical-scientific, believing to improve the efficiency and as to reduce costs. The project aimed to study the effectiveness of the mixture of glyphosate to sulfometuron methyl or ethyltrinexapac, and physiological changes in sugar cane and its implications on productivity, technological quality and in the regrowth of the ratoon cane in application beginning of the season. The study was installed and conducted in the years 2007 and 2008 in ratoon cane, in area belonging to the COSAN GROUP, Barra Unit, in Igaraçu do Tietê, São Paulo. The experimental design was in a randomized block design with five replications. Each experiment consisted of six treatments (1 - control, without application of ripener, 2 - application of glyphosate (commercial product Roundup), 3 - application of sulfometuron methyl (commercial product Curavial), 4 - application of ethyl-trinexapac (commercial product Moddus), 5 - application of the mixture glyphosate + sulfometuron methyl and 6 - application of the mixture glyphosate + ethyl-trinexapac. The doses were, respectively: 0,4 L c.p. ha-1, 20 g ha-1, 0,8 L c.p. ha-1, 0,2 L c.p. ha-1 + 15 g c.p. ha-1 e 0,2 L c.p. ha-1 + 0,5 g c.p. ha-1. The variety... (Complete abstract click electronic access below)
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Siqueira, Gabriela Ferraz de 1980. "Eficácia da mistura de glifosato a outros maturadores na cana-de-açúcar (Saccharum spp.) /." Botucatu : [s.n.], 2009. http://hdl.handle.net/11449/86398.
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Orientador: Carlos Alexandre Costa CrusciolBanca: Marcelo de Almeida Silva
Banca: Raffaella Rossetto
Resumo: A maturação natural, em início de safra, pode ser deficiente, mesmo em variedades precoces e o emprego de maturadores químicos destaca-se como uma ferramenta importante para antecipar o processo de maturação, promover melhorias na qualidade da matéria-prima a ser processada, otimizar os resultados agro-industriais e econômicos e auxiliar no planejamento da safra. Dentre os produtos utilizados, o glifosato é um dos que proporciona maior eficiência na antecipação da colheita, contudo, frequentemente diminui a rebrota da soqueira reduzindo a produtividade do canavial na safra posterior. Técnicos e produtores têm misturado o glifosato a outros maturadores, sem embasamento técnico-científico, acreditando melhorar a eficiência desses e como forma de redução de custo. O projeto teve por objetivo estudar a eficácia da mistura de glifosato a sulfometuron metil ou etil-trinexapac, e as alterações fisiológicas na cana-de-açúcar bem como suas implicações na produtividade, qualidade tecnológica e na rebrota da soqueira, em aplicação em início de safra. O projeto de pesquisa foi instalado e conduzido nos anos agrícolas de 2007 e 2008 em cana soca, em área pertencente ao GRUPO COSAN, Unidade Barra, no município de Igaraçu do Tietê, Estado de São Paulo. O delineamento experimental utilizado foi em blocos casualizados com cinco repetições. O experimento foi constituído de seis tratamentos: 1 - testemunha, sem aplicação de maturador, 2 - aplicação de glifosato (produto comercial Roundup), 3 - aplicação de sulfometuron metil (produto comercial Curavial), 4 - aplicação de etil-trinexapac (produto comercial Moddus), 5 - aplicação da mistura glifosato + sulfometuron metil e 6 - aplicação da mistura glifosato + etil-trinexapac. As doses... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The natural maturation, early harvest, may be poor, even in early varieties and the use of chemical ripeners stands out as an important tool to advance the maturation process, further improvements in the quality of raw material to be processed, optimize results agro-industrial and economic and assist in the planning of the harvest. Among the products used, glyphosate is one that provides greater efficiency in anticipation of the harvest, however, often reduces the regrowth of the ratoon cane reducing the productivity of sugarcane in crop later. Technicians and producers have mixed the glyphosate with other ripeners, without basement technical-scientific, believing to improve the efficiency and as to reduce costs. The project aimed to study the effectiveness of the mixture of glyphosate to sulfometuron methyl or ethyltrinexapac, and physiological changes in sugar cane and its implications on productivity, technological quality and in the regrowth of the ratoon cane in application beginning of the season. The study was installed and conducted in the years 2007 and 2008 in ratoon cane, in area belonging to the COSAN GROUP, Barra Unit, in Igaraçu do Tietê, São Paulo. The experimental design was in a randomized block design with five replications. Each experiment consisted of six treatments (1 - control, without application of ripener, 2 - application of glyphosate (commercial product Roundup), 3 - application of sulfometuron methyl (commercial product Curavial), 4 - application of ethyl-trinexapac (commercial product Moddus), 5 - application of the mixture glyphosate + sulfometuron methyl and 6 - application of the mixture glyphosate + ethyl-trinexapac. The doses were, respectively: 0,4 L c.p. ha-1, 20 g ha-1, 0,8 L c.p. ha-1, 0,2 L c.p. ha-1 + 15 g c.p. ha-1 e 0,2 L c.p. ha-1 + 0,5 g c.p. ha-1. The variety... (Complete abstract click electronic access below)
Mestre
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Lencina, Kelen Haygert. "MICROPROPAGAÇÃO E DIVERSIDADE GENÉTICA EM Apuleia leiocarpa (VOGEL) J. F. MACBRIDE." Universidade Federal de Santa Maria, 2016. http://repositorio.ufsm.br/handle/1/3793.
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Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorThe aim of this study was to evaluate the in vitro productivity of micro-stumps, in vitro and ex vitro rooting and acclimatization of micropropagated plantlets, and to assess the genetic diversity of Apuleia leiocarpa (Vogel) J. F. Macbride (apuleia) with RAPD markers. Micro-stumps originated from drastic pruning of aseptic seedlings were grown in WPM culture medium supplemented with 0; 2.2; 4.4; 6.6 and 8.8 μM of 6-benzylaminopurine (BAP) and sub-cultured in WPM medium without cytokinin. Apuleia micro-stumps were also grown in WPM, MS or ½ MS, with or without 1.5 g L-1 of activated charcoal. Three shoot collections were done at 30, 60 and 90 days of cultivation. Nodal segments and micro-cuttings were maintained in WPM culture medium with 0; 4.9; 9.8; 14.7 and 19.6 μM of indole butyric acid (IBA). For acclimatization, rotted nodal segments and micro-cuttings were planted in equal proportions of commercial substrate + vermiculite + coarse sand, and commercial substrate + vermiculite. For ex vitro rooting, nodal segments and micro-cuttings were treated or not with 4920 μM of IBA for 10 seconds and cultivated in equal proportions of commercial substrate + vermiculite + coarse sand, commercial substrate + vermiculite, and commercial substrate + coarse sand. For genetic analysis, DNA was extracted from leaf samples of 88 plants of apuleia. Eighteen RAPD primers were tested. The amplified fragments were separated in agarose gel of 1.2% (v/v), containing 3 μL of ethidium bromide. The fragments were marked as absence or presence, generating a binary matrix. Total polymorphism and the relative contribution of each primer for the polymorphism were calculated. The polymorphism information content (PIC) was calculated for each fragment and primer. Cluster analysis was based upon Jaccard similarity and UPGMA method. The conservation of the apuleia micro-stumps in WPM or ½ MS media supplemented with 8.8 μM of BAP and sub-cultured in culture medium without citokynin increases number and length of shoots. Maintaining micro-stumps in culture medium supplemented with activated charcoal increases micro-cuttings production, but in its absence results in callus formation and indirect organogenesis of shoots. Nodal segments were more competent than micro-cuttings for rooting in culture medium without IBA. Substrate composition does not affect survival and growth during acclimatization of in vitro produced plantlets. Nodal segments treated with 4920 μM of IBA and cultivated in commercial substrate + vermiculite + coarse sand show the best responses for ex vitro rooting. Both in vitro conservation of apuleia micro-stumps and ex vitro rooting are promising strategies for plantlet production. The RAPD is a feasible technique for genetic analysis, and it identifies high genetic variability in apuleia.
Os objetivos deste trabalho foram avaliar a produtividade de microcepas mantidas in vitro, o enraizamento e a aclimatização de plantas micropropagadas, assim como avaliar a diversidade genética em Apuleia leiocarpa (Vogel) J. F. Macbride (grápia) com uso de marcadores RAPD. Microcepas oriundas da poda drástica de plantas assépticas foram cultivadas em meio de cultura WPM acrescido de 0, 2,2, 4,4, 6,6 e 8,8 μM de 6-benzilaminopurina (BAP) e subcultivadas em meio de cultura WPM sem citocinina. Microcepas também foram cultivadas em meio de cultura WPM, MS ou ½ MS, acrescido ou não de 1,5 g L-1 de carvão ativado e submetidas a três coletas de brotos aos 30, 60 e 90 dias de cultivo. No enraizamento in vitro, segmentos nodais e microestacas foram mantidos em meio de cultura WPM com 0, 4,9, 9,8, 14,7 e 19,6 μM de ácido indolbutírico (AIB). Na aclimatização das plantas foram testadas as composições de substrato comercial + vermiculita + areia grossa e substrato comercial + vermiculita, em iguais proporções. Para o enraizamento ex vitro, segmentos nodais e microestacas foram tratados ou não com 4920 μM de AIB por 10 segundos e cultivados em iguais proporções de substrato comercial + vermiculita + areia grossa, substrato comercial + vermiculita e substrato comercial + areia grossa. Para as análises RAPD, o DNA foi extraído de amostras foliares de 88 plantas. Foram avaliados 18 iniciadores, sendo a visualização dos fragmentos realizada em gel de agarose preparado a 1,2% (p/v), contendo 3 μL de brometo de etídio e submetido a eletroforese. Os fragmentos foram pontuados com ausência ou presença gerando uma matriz binária. Foi calculada a contribuição relativa de cada iniciador para o polimorfismo bem como o polimorfismo total. Também foi calculado o conteúdo de informação para o polimorfismo (PIC) para cada fragmento e por iniciador. A análise de agrupamento foi realizada com base na similaridade de Jaccard e no método UPGMA. A manutenção das microcepas de grápia em meio de cultura WPM ou ½ MS suplementado com 8,8 μM de BAP, seguido do subcultivo em meio de cultura sem citocinina aumenta o número e comprimento das brotações. O cultivo de microcepas em meio de cultura com carvão ativado aumenta a produção de microestacas por microcepas, enquanto a ausência de carvão ativado favorece a formação de calos e brotos por organogênese indireta. Quanto ao enraizamento in vitro, os segmentos nodais apresentaram maior resposta do que microestacas, sendo necessária a suplementação do meio de cultura com AIB. A composição do substrato não afetou a sobrevivência e o crescimento das plantas produzidas in vitro durante a aclimatização. As melhores respostas de enraizamento ex vitro foram obtidas com segmentos nodais, assim como com explantes tratados com 4920 μM de AIB e cultivados em substrato comercial + vermiculita + areia grossa. Tanto a manutenção in vitro de microcepas quanto o enraizamento ex vitro são técnicas promissoras para a produção de plantas de grápia. O marcador RAPD é eficiente para a análise genética e detectou alta variabilidade genética na grápia.
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MacGregor, Paula. "Assessing stumpy formation and stumpy-specific gene expression in Trypanosoma brucei." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/4922.
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During the bloodstream stage of the Trypanosoma brucei lifecycle, the parasite exists in two different states: the proliferative slender form and the non-proliferative, transmissible, stumpy form. The transition from the slender to stumpy form is stimulated by a density-dependent mechanism and is important in infection dynamics, ordered antigenic variation and disease transmissibility. The slender to stumpy transition and the contribution of stumpy formation to within-host dynamics have been difficult to analyse, however, because cell-type specific markers have been restricted to imprecise morphological criteria. PAD1 is a recently identified stumpy-specific protein which acts as a molecular marker for stumpy formation and a functional marker for transmission. Here, the control of stumpy-specific gene expression via the 3’UTR has been analysed, identifying that there are repressive elements in the 3’UTR preventing inappropriate expression during the slender life stage. Further, both pleomorphic and monomorphic transgenic reporter cell lines utilising the PAD1 3’UTR have been created that report on stumpy formation in vitro and these have been used for the analysis of stumpyinducing chemical compounds. Finally, a sensitive and accurate qRT-PCR assay has been developed and optimised that faithfully reports both parasitaemia and stumpy formation throughout host infection. Using a chronic infection rodent model, stumpy levels have been monitored on the basis of conventional morphological and cell cycle assays, as well as by qRT-PCR for PAD1 expression. The results define the temporal order of events that result in the generation of stumpy forms early in a parasite infection and thereafter describe the dynamics of slender and stumpy forms in chronic infections extending over several weeks. This quantitative data has allowed the mathematical modelling of transmission competence in trypanosome infections, suggesting dominance of transmission stages throughout infection.
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Rizzo, Julio B. De. "Sobre a influência de C. Stumpf para a terceira investigação lógica de E. Husserl e seu significado para a fenomenologia nascente." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/8/8133/tde-27032015-152152/.
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A pesquisa pretende investigar o significado e a origem da influência de Carl Stumpf na terceira investigação lógica intitulada Sobre a teoria dos todos e das partes(Zur Lehre von den Ganzen und Teilen), publicada em 1901, buscando relacioná-la ao desenvolvimento anterior do pensamento de Husserl. Serão abordados o surgimento dos conceitos de auto-suficiência (Selbständigkeit) e não auto-suficiência (Unselbständigkeit) no contexto do problema da origem da representação de espaço e da obra de Stumpf referida ao tema; a interpretação de Husserl destes conceitos; e seu papel sistemático do ponto de vista da evolução da filosofia husserliana em seu período inicialThis research intends to investigate the meaning and origin of the influence of Carl Stumpf on the third logical investigation, entitled On the theory of Wholes and Parts(Zur Lehre von den Ganzen und Teilen), published in 1901, trying to relate it do the former development of Husserls thought. It will be taken into account the birth of the concepts of independence (Selbständigkeit) and dependence (Unselbständigkeit) in the context of the problem of the origin of the representation of space and Stumpfs work related to this theme; the interpretation Husserl gives to these concepts; and their systematic role from the point of view of the evolution of the husserlian philosophy on its initial period
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Stumpf, Sören [Verfasser]. "Formelhafte (Ir-)Regularitäten / Sören Stumpf." Frankfurt : Peter Lang GmbH, Internationaler Verlag der Wissenschaften, 2015. http://d-nb.info/1080460586/34.
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Ewen, Wolfgang. "Carl Stumpf und Gottlob Frege." Würzburg Königshausen & Neumann, 2007. http://d-nb.info/990585565/04.
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Kloditz, Roger [Verfasser], Thorsten [Akademischer Betreuer] Stumpf, Thorsten [Gutachter] Stumpf, Michael [Akademischer Betreuer] Patzschke, and Thomas [Gutachter] Heine. "Quantenchemische Berechnungen an tetravalenten ƒ-Elementverbindungen / Roger Kloditz ; Gutachter: Thorsten Stumpf, Thomas Heine ; Thorsten Stumpf, Michael Patzschke." Dresden : Technische Universität Dresden, 2021. http://d-nb.info/1235346579/34.
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Rudolphi, Jörgen. "Bryophytes, lichens and dead wood in young managed boreal forests /." Uppsala : Dept. of Ecology, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/200788.pdf.
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Collison, Jeff. "Soil disturbance resulting from stump harvesting." Thesis, University of Stirling, 2014. http://hdl.handle.net/1893/20936.
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Forest biomass burned for energy purposes does not need to be accounted for under IPCC rules. This has led to a number of countries considering tree stump harvesting as a source of forest biomass. However there are concerns that the soil disturbance that this may entail could have adverse environmental effects, including the loss of sequestered carbon from the soil. Published results differ in the degree and nature of stump harvesting soil disturbance. Two widely used measures employed in stump harvesting soil disturbance studies are visual assessment of disturbance extent and bulk density measures of the nature of disturbance. Each of these has limitations. This study seeks to extend the insight into both the nature and extent of soil disturbance resulting from stump harvesting by the application of additional techniques. In this way the physical effects of soil disturbance by stump harvesting will be compared with those of other forestry practices. To overcome the two-dimensional and subjective nature of visual assessment, a radiometric approach was adopted, utilising residual Chernobyl 137Cs fallout to determine the degree of soil mixing. To complement bulk density measurements, micromorphological analyses of soil thin sections taken from field samples were carried out to investigate the impact of compressive force on pore space. Low-cost tracer devices were deployed in the soil around stumps prior to extraction to permit the monitoring of the lateral movement of soil during stump extraction. These methods were applied to a stump harvesting operation carried out under current UK guidance at a UPM Tilhill managed site in south west Scotland. The radiometric method demonstrated its capacity to recognise differing degrees of soil disturbance in an operational forest environment, including some disturbance that might escape visual assessment. Analysis of soil thin sections provided the evidence of a significant increase in the pore capacity of disturbed soil. The soil movement tracers developed for this project provided the capability to examine the various trajectories of soil during stump extraction as well as dimensioning the resulting disturbance crater. The study indicated that under current UK management and operational practice, stump harvesting generated a higher level of soil disturbance compared to ground preparation by trench mounding, with an estimated 1260 m3 ha-1 of soil disturbed by stump harvesting compared to 250 m3 ha-1 from trench mounding. Stump harvesting was found to generate a net reduction in soil bulk density in the affected areas, contrary to the findings of some other studies. This outcome is dependent on adhering to particular site management and operational procedures. The practice of raking over the site following stump harvesting is estimated to add a further 10% to the volume of soil disturbed, and is a questionable activity under soil sustainability guidance. This work was part-funded and actively supported by the UK Forestry Commission and UPM Tilhill.
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Fichter, Sebastian [Verfasser], Thorsten [Gutachter] Stumpf, Thorsten [Akademischer Betreuer] Stumpf, Peter W. [Gutachter] Roesky, and Juliane [Akademischer Betreuer] März. "Synthesis and Characterization of Tri- and Tetravalent Actinide Amidinates / Sebastian Fichter ; Gutachter: Thorsten Stumpf, Peter W. Roesky ; Thorsten Stumpf, Juliane März." Dresden : Technische Universität Dresden, 2020. http://d-nb.info/1227833059/34.
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Lindhe, Anders. "Conservation through management : cut wood as substrate for saproxylic organisms /." Uppsala : Dept. of Entomology, Swedish Univ. of Agricultural Sciences, 2004. http://epsilon.slu.se/s300.pdf.
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Stumpf, Kai Walter [Verfasser]. "Germyliden- und Germylidin-Komplexe des Molybdäns / Kai Walter Stumpf." Bonn : Universitäts- und Landesbibliothek Bonn, 2014. http://d-nb.info/1080561137/34.
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Stumpp, Stefan [Verfasser]. "Management des Crowdsourcing-Prozesses in der Organisation / Stefan Stumpp." Baden-Baden : Nomos Verlagsgesellschaft mbH & Co. KG, 2019. http://d-nb.info/1183474601/34.
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Polesi, Natalia Pimentel Esposito. "Estudo da comunidade bacteriana endofítica e de sua manifestação na micropropagação de Eucalyptus benthamii." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-22092015-143023/.
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Eucalyptus benthamii tem se mostrado especialmente vantajoso como alternativa ao cultivo em regiões frias, justificando esforços para o estabelecimento de protocolos para sua micropropagação. Porém, as matrizes são preferencialmente selecionadas quando adultas (material apresenta menor competência morfogênica), tornando a micropropagação dependente de maior número de subcultivos e maior tempo para se reverter o material ao rejuvenescimento. Assim, a redução das perdas in vitro tem merecido atenção, como por exemplo, as manifestações endofíticas, que exigem maximização da eficiência da cultura e adequações no protocolo, visando minimizá-las, possibilitando melhorar o entendimento das relações estabelecidas e mantidas entre os endófitos e seu hospedeiro durante a micropropagação. Dessa maneira, foram utilizadas minicepas provenientes de duas fontes de miniestacas coletadas a partir do brotamento de gemas epicórmicas de megaestacas da base da copa e de brotamentos do anelamento da base do tronco, de uma matriz de E. benthamii com 13 anos de idade, estabelecidas em minijardim clonal sob condição de casa de vegetação, com o objetivo de avaliar como se dá a multiplicação, sob diferentes condições de cultivo, das duas fontes de explantes (minicepas); analisar se a frequência e intensidade das manifestações endofíticas são afetadas pelas diferentes condições de cultivo; investigar a ocorrência de alterações na comunidade bacteriana endofítica devido à alteração das condições de cultivo e fase da micropropagação (in vivo = minicepas, e in vitro = microcepas, material alongado e enraizado). Visando atender estes objetivos, a pesquisa se dividiu em duas partes. Na primeira (capitulo 3) o desenvolvimento, os aspectos morfofisiológicos, histoquímicos e a manifestação endofítica foram avaliados na multiplicação das duas fontes de explante sob diferentes meios e condições de cultivo. Na segunda (capítulo 4) as comunidades bacterianas endofíticas foram analisadas por meio de PCR-DGGE, baseada na região V6 do gene 16S DNAr. Os resultados mostraram que as microcepas provenientes de megaestaca tiveram melhor desenvolvimento independentemente do tratamento e maior frequência de manifestações endofíticas, comparando-se com as de anelamento. As comunidades bacterinas endofíticas foram distintas entre as amostras in vivo e in vitro, e se alteraram ao longo dos subcultivos e nas amostras alongadas e enraizadas. As diferenças existentes no desenvolvimento das microcepas podem ser inerentes à totipotencialidade do material, mas também podem ser afetadas, tanto pela ocorrência de manifestação, quanto pela comunidade bacteriana endofítica mais ou menos sensível ao processo de micropropagação, auxiliando ou prejudicando o desenvolvimento in vitro de seus hospedeiros. Cabe destacar, ainda, que mesmo em um sistema asséptico e ambientalmente controlado, os microrganismos endofíticos que resistiram a todo processo de desinfestação e cultivo, não estão \"adormecidos\", muito pelo contrário podem se alterar em quantidade à medida que seu hospedeiro é submetido a um novo sistema de cultivo (introdução) ou uma nova fase dentro da micropropagação (multiplicação → alongamento e enraizamnento) ou, ainda, ao longo dos subcultivos. Sendo assim, a complexa rede de relações das plantas com seus endófitos não cessa durante o cultivo in vitro, ao contrário mantém-se dinâmica.Eucalyptus benthamii has proven to be especially advantageous as an alternative culture in cold regions, justifying efforts to establish protocols for micropropagation. However, the matrices are preferably selected when adults (material with lower morphogenic efficiency), making micropropagation more dependent of subcultures and too longer to reverse the material to rejuvenation. Thus, reduction of losses in vitro has deserved attention, such for example the endophytic manifestations that require the maximization of efficiency culture and adjustments to the Protocol, in order to minimize them, enabling better understanding of the relations established and maintained between endophytes and its host along micropropagation. For this, mini-stumps were used from two sources of mini-cuttings collected from the epicormic shoots of mega-cuttings from the treetop base and shoots from girdling from the trunk base, both of one E. benthamii matrix with 13 years of age established in clonal mini garden under greenhouse condition, aimed to evaluate how is the multiplication of two sources of explants (mini-stumps) under different growing conditions; analyze how the endophytic manifestations frequency and intensity are affected by different conditions; investigate the changes to occurrence in the endophytic bacterial community due to the variation of culture conditions and micropropagation phase (in vivo = mini-stumps, and in vitro = micro-stumps, elongated and rooted materials). In order to meet these objectives, the research was divided in two parts. In the first (chapter 3) the development, morphophysiological aspects, histochemical and endophytic manifestation were evaluated in the multiplication of the two explants sources from different media and culture conditions. In the second (chapter 4) endophytic bacterial communities were analyzed by PCR-DGGE based on the V6 region of 16S rDNA gene. The results showed that micro-stumps from mega-cuttings had better development regardless of treatment and increased frequency of endophytic events, comparing with the girdling. Endophytic bacterial communities were different between samples in vivo and in vitro, and have changed over the subcultures and the elongated and rooted samples. The differences in the development of micro-stumps can be explained by the totipotentiality inherent to the material, but may also be affected by both the manifestation occurrence and the endophytic bacterial community more or less sensitive to the micropropagation, helping or harming the in vitro development of their hosts. We also highlight that even in an aseptic and environmentally controlled system, the endophytic microorganisms that resisted the whole process of disinfection and cultivation, are not \"asleep\", quite the opposite may change in quantity when your host is subjected to a new cultivation system (in vitro establishment) and a new phase within the micropropagation (multiplication → stretching and enraizamnento), or even along the subcultures. This way, the complex network of relationships of the plant with their endophyte does not cease during the in vitro culture, unlike remains dynamic.
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Husar, Richard [Verfasser], Thorsten [Akademischer Betreuer] Stumpf, and Alexander [Akademischer Betreuer] Eychmüller. "Investigation into the Formation of Nanoparticles of Tetravalent Neptunium in Slightly Alkaline Aqueous Solution / Richard Husar. Gutachter: Thorsten Stumpf ; Alexander Eychmüller. Betreuer: Thorsten Stumpf." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://d-nb.info/1076280099/34.
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Lu, Qian. "Contaminants in food stuffs." Thesis, Liverpool John Moores University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388586.
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Lafforgue, Laurent. "D-stukas de drinfeld." Paris 11, 1994. http://www.theses.fr/1994PA112280.
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Dans un premier chapitre on definit, pour d une algebre a division centrale sur un corps de fonctions sur un corps fini, les d-stukas de drinfeld de rang quelconque avec structure de niveau ainsi que leurs champs classifiants. On montre que ces champs sont representables au sens de deligne-mumford, localement de type fini et lisses sur leur base naturelle. Ils sont munis de deux morphismes dits de frobenius partiels et de correspondances de hecke. Dans le chapitre deux, on introduit une notion de filtration canonique de harder-narasimhan pour les d-stukas. Elle permet d'ecrire de facon naturelle les champs classifiant les d-stukas comme des reunions filtrantes d'ouverts de type fini. Dans le chapitre trois, on decrit les groupoides de points fixes des composes de morphismes de frobenius partiels et de correspondances de hecke. Quand on tronque comme dans le chapitre deux, ces groupoides deviennent finis et en comptant leurs points avec multiplicites, on obtient des rationnels, baptises nombres de lefschetz tronques pour lesquels on donne une premiere formule. Le chapitre quatre traite le cas du rang un. Les champs classifiants correspondants sont alors projectifs. On precise la structure de leur cohomologie -adique en combinant la formule des points fixes de grothendieck-lefschetz et la formule des traces de selberg. Dans le chapitre cinq, on etudie le cas des rangs superieurs. On introduit une facon de definir des traces tronquees pour les operateurs de hecke agissant sur les espaces de fonctions automorphes attaches aux groupes lineaires sur d. On prouve notre theoreme principal qui affirme que les nombres de lefschetz tronques introduits au chapitre trois sont egaux aux traces tronquees de certains operateurs de hecke
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Mortimer, Clare Michelle. "Pain management in the rehabilitation of amputees : an investigation of current service provision and assessment of unmet need in patient support and information." Thesis, Queen Margaret University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327080.
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Schalau, Jeff. "Cut Stump Application of Herbicides to Manage Woody Vegetation." College of Agriculture and Life Sciences, University of Arizona (Tucson, AZ), 2006. http://hdl.handle.net/10150/144792.
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2 pp.Persistent woody plants can sometimes conflict with gardening and landscape goals. In many cases, plant removal becomes necessary. This may be accomplished through manual stump removal or the use of herbicides. With some knowledge of the life history of the target plant, cut stumps can be safely and effectively treated with herbicides to prevent regrowth. Species lists, safety tips, examples, photos, and non-herbicide alternatives are provided to ensure optimum stump killing success.
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Schalau, Jeff. "Cut Stump Application of Herbicides to Manage Woody Vegetation." College of Agriculture and Life Sciences, University of Arizona (Tucson, AZ), 2011. http://hdl.handle.net/10150/239596.
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Obeid, Muhammad Hassan [Verfasser], Thorsten [Akademischer Betreuer] Stumpf, Karim [Akademischer Betreuer] Fahmy, and Gert [Akademischer Betreuer] Bernhard. "Assessment of Low-Dose Radiotoxicity in Microorganisms and Higher Organisms / Muhammad Hassan Obeid. Betreuer: Thorsten Stumpf ; Karim Fahmy ; Gert Bernhard. Gutachter: Thorsten Stumpf ; Karim Fahmy." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://d-nb.info/1088185576/34.
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Ulbricht, Isabell [Verfasser], Thorsten [Akademischer Betreuer] [Gutachter] Stumpf, and Alexander [Gutachter] Eychmüller. "Bildung von Kolloiden des tetravalenten Urans unter Einfluss von Silikat in neutralen und schwachalkalischen wässrigen Systemen / Isabell Ulbricht ; Gutachter: Thorsten Stumpf, Alexander Eychmüller ; Betreuer: Thorsten Stumpf." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://d-nb.info/1119363314/34.
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Zilch, Josef. "In vitro Untersuchung zur Versorgung avitaler Prothesenpfeilerzähne mit Stift-Stumpf-Aufbauten." Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-75344.
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Stumpf, Christopher [Verfasser]. "Hochfrequenztechnische Modellierung und Entwicklung von Empfangsantennenarrays für die Magnetresonanztomographie / Christopher Stumpf." München : Verlag Dr. Hut, 2018. http://d-nb.info/1168534186/34.
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Monk, Stephanie Lydia Spencer. "Translational control and the escape from translational arrest in stumpy form Trypanosoma brucei." Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/8177.
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The transmission of Trypanosoma brucei, the causative agent of human African trypanosomiasis, depends upon the development in the bloodstream of 'stumpy forms' from non-transmissible 'slender forms'. In stumpy forms many mRNAs are downregulated and translation is generally repressed. However, a small subset of genes escape this repression and are upregulated, presumably as an adaptation for transmission. To understand the basic of this, regulatory sequences within the 3'UTR of a major stumpy-enriched transcript (an ESAG9 gene) have been characterised. This identified a signal responsible for gene silencing in slender forms and gene activation when cells develop to stumpy forms. An investigation was made of upstream open reading frames (uORFs) as a mechanism for the control of stumpy form gene expression. No evidence was found of uORF control, but one gene investigated was found to produce two transcripts through trans-splicing at different sites. These transcripts, which were found to exhibit some differential abundance between life-cycle stages, would generate a long and short form (from an internal ATG) of the encoded protein. Both are predicted to contain a UBA/TS-N (ubiquitin associated) domain, however, the longer form of the protein is also predicted to contain a transmembrane helix and cleavable signal peptide, suggesting a different localisation. However, ectopic expression of either protein form with a Ty epitope tag resulted in the same protein localisation. Additionally, the transcripts of two translational protein homologues, TbeIF4E4 and TbeIF6, were identified as upregulated in stumpy forms. Radiolabelled-methionine experiments and polysome analysis showed that overexpression or RNAi-mediated ablation of TbeIF6 resulted in a decrease in protein synthesis and decrease in translation. Unlike its archaeal homologue, TbeIF6 protein was not induced by coldshock treatment. Finally, to identify which transcripts escape translational repression in stumpy forms an analysis was made of polysome-associated transcripts by RNA-sequencing. This identified potentially interesting genes for further investigation, and showed that many procyclic-enriched transcripts were also enriched in stumpy form polysomeassociated RNA, confirming these cells as preadapted for transmission. Together, this work has characterised a 3’UTR regulatory element in a stumpy-enriched transcript, examined alternative trans-splicing of another transcript, investigated two translational protein homologues and identified transcripts that escape translational repression in the transmissible life-cycle stage of T. brucei.
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Stumpf, Dana [Verfasser]. "Frühkindliche Karies: Wissen und Handeln bei Eltern von kleinen Kindern / Dana Stumpf." Greifswald : Universitätsbibliothek Greifswald, 2012. http://d-nb.info/1021952818/34.
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Braun, Sebastian [Verfasser], Eike [Akademischer Betreuer] Stumpf, and Wolfgang [Akademischer Betreuer] Schröder. "Numerische Simulation von Wirbelaufrollvorgängen an Tragflügeln / Sebastian Braun ; Eike Stumpf, Wolfgang Schröder." Aachen : Universitätsbibliothek der RWTH Aachen, 2016. http://d-nb.info/1130792579/34.
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Stumpf, Teresa [Verfasser]. "Regime urbaner Resilienz: Sozialräumliche Dynamiken des Wandels in belasteten Stadtbezirken / Teresa Stumpf." Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2019. http://d-nb.info/1233786040/34.
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Körner, Stephan [Verfasser], Eike [Akademischer Betreuer] Stumpf, and Ch [Akademischer Betreuer] Breitsamter. "Multi-Model Ensemble Wake Vortex Prediction / Stephan Körner ; Eike Stumpf, Ch. Breitsamter." Aachen : Universitätsbibliothek der RWTH Aachen, 2017. http://d-nb.info/116245122X/34.
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McDonald, Lindsay Mary. "Ordering components of the slender to stumpy signalling pathway in Trypanosoma brucei." Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/19573.
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In the mammalian bloodstream, the protozoan parasite Trypanosoma brucei undergoes differentiation from proliferative slender forms to arrested, transmissible, stumpy forms. This transition is associated with extensive cytological and metabolic changes that promote survival in the tsetse midgut, and also influences infection dynamics within the mammalian host. A number of genes involved in this transformation were recently identified using an RNAi library screen for resistance to pCPTcAMP, a membrane-permeable cyclic AMP analogue that induces differentiation. These molecules, referred to here as posST (positive mediators of STumpy formation), were thereafter validated to regulate the slender to stumpy transition, with many of them apparently comprising part of a signal transduction and effector pathway. However, it is unknown how these proteins act in relation to one another or are ordered within the pathway. To this end, null mutants were created for several posST components in differentiation-competent pleomorphic trypanosomes and, in this genetic background, other members of the predicted pathway expressed to test their ability to restore stumpy formation. Analysis of distinct combinations has been used to build a preliminary pathway structure model for the signalling events underlying trypanosome quorum sensing. In addition, phosphoproteomic analysis of two null mutants has revealed downstream signalling effects of two posST kinases, MEKK1 and YAK. A similar extragenic suppression approach was also applied to explore the interaction between the identified drivers of stumpy formation and the target of rapamycin kinase, TOR4, which has previously been shown to act as a negative regulator of stumpy formation in monomorphs. Dual ablation of TOR4 and posST components revealed insight into the intersection of stumpy-promoting and stumpy-inhibiting pathways. Finally, a chemical-genetic approach was used to investigate the posST pathway using two differentiation-inducing compounds: the previously studied E667, and GKI7, newly identified from a kinase inhibitor set. RNAi lines for different posST components were tested for their ability to undergo development in the presence of these compounds. An RNAi library screen using GKI7 identified putative new mediators of stumpy formation.
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Braun, Sebastian [Verfasser], Eike Akademischer Betreuer] Stumpf, and Wolfgang [Akademischer Betreuer] [Schröder. "Numerische Simulation von Wirbelaufrollvorgängen an Tragflügeln / Sebastian Braun ; Eike Stumpf, Wolfgang Schröder." Aachen : Universitätsbibliothek der RWTH Aachen, 2016. http://nbn-resolving.de/urn:nbn:de:hbz:82-rwth-2016-116788.
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Tamimi, Ehab A., Jeffrey D. Pyne, Dominic K. Muli, Katelyn F. Axman, Stephen J. Howerton, Matthew R. Davis, Christopher A. Girkin, and Geest Jonathan P. Vande. "Racioethnic Differences in Human Posterior Scleral and Optic Nerve Stump Deformation." ASSOC RESEARCH VISION OPHTHALMOLOGY INC, 2017. http://hdl.handle.net/10150/626003.
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PURPOSE. The purpose of this study was to quantify the biomechanical response of human posterior ocular tissues from donors of various racioethnic groups to better understand how differences in these properties may play a role in the racioethnic health disparities known to exist in glaucoma. METHODS. Sequential digital image correlation (S-DIC) was used to measure the pressure-induced surface deformations of 23 normal human posterior poles from three racioethnic groups: African descent (AD), European descent (ED), and Hispanic ethnicity (HIS). Regional in-plane principal strains were compared across three zones: the optic nerve stump (ONS), the peripapillary (PP) sclera, and non-PP sclera. RESULTS. The PP scleral tensile strains were found to be lower for ED eyes compared with AD and HIS eyes at 15 mm Hg (P = 0.024 and 0.039, respectively). The mean compressive strains were significantly higher for AD eyes compared with ED eyes at 15 mm Hg (P = 0.018). We also found that the relationship between tensile strain and pressure was significant for those of ED and HIS eyes (P < 0.001 and P = 0.004, respectively), whereas it was not significant for those of AD (P = 0.392). CONCLUSIONS. Our results suggest that, assuming glaucomatous nerve loss is caused by mechanical strains in the vicinity of the optic nerve head, the mechanism of increased glaucoma prevalence may be different in those of AD versus HIS. Our ONS strain analysis also suggested that it may be important to account for ONS geometry and material properties in future scleral biomechanical analysis.
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Waddell, David Andrew. "A practical system for determining optimal tree bucking at the stump." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27740.
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The objective of this study is to develop a practical system for determining optimal bucking patterns at the stump. The log bucking system was developed for implementation on an IBM-PC micro-computer using data for western hemlock (Tsuga heterophylla (Raf.) Sarg.).
The log bucking system is based on a two-phase optimization algorithm which utilizes dynamic programming-to determine the optimal bucking pattern with the objective of maximizing potential tree value. The first phase determines the ultimate bucking pattern within the tree such that potential gross value of derived products is maximized. This pattern reflects log lengths that would be cut if the tree were at the mill. The second phase determines which of the first phase optimal cuts should be made at the stump at the time of tree felling such that extraction costs are minimized. The solution to these two phases yields a bucking pattern that maximizes potential net value from the tree. The log bucking system incorporates assessment of log grade, assignment of value based on log grade and premium log lengths, consideration of standard product lengths, and consideration of extraction costs. The log bucking system includes an algorithm for evaluating unusual bucking situations when there are practical constraints imposed on saw cut locations.
For a set of test data, the potential net value of logs bucked using the optimization algorithm within the log bucking system showed an increase of 4.2% over the potential net value of trees bucked using present industry bucking practices. A two way analysis of variance for paired observations determined that this increase was significant at a 0.05% level of significance.Forestry, Faculty of
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Du, Toit Lindie. "Some applications of Sturm's comparison theorem." Diss., University of Pretoria, 2012. http://hdl.handle.net/2263/30793.
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We consider several classes of orthogonal polynomials as well as the Bessel function where we study the convexity of zeros of these polynomials, satisfying either differential or real difference equations, by applying Sturm's comparison and convexity theorems as well as analogues of these theorems. In addition several results are obtained concerning the distances between consecutive zeros of some of these classes of polynomials. Further research possibilities concerning q-polynomials and polynomials satisfying complex difference equations are discussed.Dissertation (MSc)--University of Pretoria, 2012.
Mathematics and Applied Mathematics
MSc
Unrestricted
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Dean, Samuel. "Functional analysis of a family of proteins implicated in Trypanosoma brucei lifecycle progression." Thesis, University of Edinburgh, 2008. http://hdl.handle.net/1842/4002.
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Bloodstream trypanosomes initiate differentiation to procyclic forms in response to a citrate/ cis-aconitate (CCA) signal. A cell line was previously selected (“defective in differentiation-clone 1”; DiD1) that was unable to differentiate to procyclic forms (Tasker et al. (2000)). Additionally, expression profiling of this line in comparison to the parental line by macroarray hybridisation identified two differentially-expressed transcripts from an 8 gene cluster of highly homologous genes we named PAD genes (Proteins Associated with Differentiation). Members of this family show distinct expression profiles throughout the trypanosome lifecycle at both the mRNA and protein level, and are localised to the cell surface membrane of the cell. At least 1 member of the family (PAD1) shows stumpy form specific RNA and protein expression, representing the first useful molecular marker for this stage, and exhibits biochemical specificity for citrate. Additionally, another member of this family (PAD2) is upregulated in response to low temperature, a condition reported to cause hypersensitivity to CCA. Finally, RNAi mediated ablation of the PAD gene transcripts compromised the capacity of stumpy form trypanosomes to differentiate to the procyclic form in response to CCA. These combined expression, cytological, reverse-genetic and biochemical data make PAD proteins excellent candidates for recognition of the signal to initiate differentiation in response to CCA.
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Gaier, Robyn Renee. "Understanding God's justice towards those who suffer : a critique of Eleonore Stump's defense /." Ohio : Ohio University, 2004. http://www.ohiolink.edu/etd/view.cgi?ohiou1107802929.
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Kottkamp, Heino. "Der Stupa als Repräsentation des buddhistischen Heilsweges : Untersuchungen zur Entstehung und Entwicklung architektonischer Symbolik /." Wiesbaden : O. Harrassowitz, 1992. http://catalogue.bnf.fr/ark:/12148/cb40026337h.
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