Relevant bibliographies by topics / Streptomyce

Academic literature on the topic 'Streptomyce'

Author: Grafiati
Published: 9 March 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Contents

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Streptomyce.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Streptomyce"

1

Burtseva, S. A., M. N. Byrsa, I. I. Shibaeva, A. Shibaev, and A. S. Sidorenko. "EFFECT OF THE MAGNETIC FIELD ON THE GROWTH AND SURVIVAL OF THE STREPTOMYCES STREPTOMYCES CANOSUS CNMN-AC-02 STRAIN." Visnyk Universytetu “Ukraina”, no. 1 (28) 2020 (2020): 46–55. http://dx.doi.org/10.36994/2707-4110-2020-1-28-04.

Full text
Abstract:
The work is devoted to the study of the effect of a low-frequency magnetic field of low intensity on microorganisms. We proceeded from the fundamental fact that the Earth's magnetic field (natural electromagnetic background) is the most important environmental factor affecting all vital processes of living organisms. It is important to realize that in the modern world the negative anthropogenic impact on the natural electromagnetic background has significantly increased due to the rapidly growing number of sources of technogenic fields. The relevance of research is due to the great scientific and practical interest in the issues of survival and growth of colonies of microorganisms under the influence of a magnetic field. We set ourselves the goal of studying the action, in particular, of a low-frequency magnetic field of low intensity. Our task was to find out how the key characteristics of the culture of microorganisms (growth and survival) change under the influence of different field values. We used the device "Biostimulus 1", developed by us at IEINT, which makes it possible to influence biological material with a low-frequency magnetic field of low intensity at specified values. The microbiological material was investigated - Streptomyce canosus CNMN-Ac-02 in the form of an aqueous suspension; an aqueous suspension pounded on the surface of agar and in lyophilic form. Exposure time: 10, 15 and 20 minutes. As a result, it was found that when an aqueous suspension of Streptomyce canosus CNMN-Ac-02 was treated with a magnetic field for 10 and 15 minutes, the appearance of colonies of 2 types with mycelium of different color and size was observed. With an exposure of 20 min. colonies of 4 types arise with the peculiarity of some colonies to release a pigment of a new color into the medium. In the case of a culture in the form of a suspension pounded on agar, similar results were obtained. The survival rate of the S.canosus CNMN-Ac-02 streptomycete strain after exposure to a magnetic field depends on the treatment time, as well as on the state of the culture (dry lyophilized culture or its aqueous suspension).
APA, Harvard, Vancouver, ISO, and other styles
2

Cuadrado, Y., M. Fern�ndez, E. Recio, J. F. Aparicio, and J. F. Mart�n. "Characterization of the ask?asd operon in aminoethoxyvinylglycine-producing Streptomyce s sp. NRRL 5331." Applied Microbiology and Biotechnology 64, no. 2 (April 1, 2004): 228–36. http://dx.doi.org/10.1007/s00253-003-1440-2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Li, Qin, Baoguo Sun, Huiyong Jia, Jie Hou, Ran Yang, Ke Xiong, Youqiang Xu, and Xiuting Li. "Engineering a xylanase from Streptomyce rochei L10904 by mutation to improve its catalytic characteristics." International Journal of Biological Macromolecules 101 (August 2017): 366–72. http://dx.doi.org/10.1016/j.ijbiomac.2017.03.135.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Li, Qin, Baoguo Sun, Xiuting Li, Ke Xiong, Youqiang Xu, Ran Yang, Jie Hou, and Chao Teng. "Improvement of the catalytic characteristics of a salt-tolerant GH10 xylanase from Streptomyce rochei L10904." International Journal of Biological Macromolecules 107 (February 2018): 1447–55. http://dx.doi.org/10.1016/j.ijbiomac.2017.10.013.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Pospíšil, Stanislav, Věra Přikrylová, Jan Němeček, and Jaroslav Spížek. "Oxidation and amidation of salicylate by Streptomyces species." Canadian Journal of Microbiology 42, no. 8 (August 1, 1996): 867–69. http://dx.doi.org/10.1139/m96-111.

Full text
Abstract:
Seven streptomycete strains were tested for biotransformation of salicylate. The products were identified by nuclear magnetic resonance spectroscopy and three types of conversion were found. Streptomyces cinnamonensis and Streptomyces spectabilis formed gentisate and salicylamide concurrently. Streptomyces rimosus transformed salicylate to salicylamide. Streptomyces lividans, Streptomyces coelicolor, Streptomyces griseus and Streptomyces avermitilis produced only gentisate. Time course studies of salicylate conversion by thin-layer chromatography and high pressure liquid chromatography showed that salicylamide was accumulated in the culture broth, whereas gentisate was further metabolized.Key words: salicylate, gentisate, salicylamide, biotransformation, Streptomyces spp.
APA, Harvard, Vancouver, ISO, and other styles
6

Le, Khanh Duy, Nan Hee Yu, Ae Ran Park, Dong-Jin Park, Chang-Jin Kim, and Jin-Cheol Kim. "Streptomyces sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases." Microorganisms 10, no. 4 (April 8, 2022): 791. http://dx.doi.org/10.3390/microorganisms10040791.

Full text
Abstract:
Bacteria and fungi are major phytopathogens which substantially affect global agricultural productivity. In the present study, Streptomyces sp. AN090126, isolated from agricultural suppressive soil in Korea, showed broad-spectrum antagonistic activity against various phytopathogenic bacteria and fungi. In the 96-well plate assay, the fermentation filtrate of Streptomyces sp. AN090126 exhibited antimicrobial activity, with a minimum inhibitory concentration (MIC) of 0.63–10% for bacteria and 0.63–3.3% for fungi. The MIC of the partially purified fraction was 20.82–250 µg/mL for bacteria and 15.6–83.33 µg/mL for fungi. Gas chromatography–mass spectrometry (GC-MS) analysis revealed that AN090126 produced various volatile organic compounds (VOCs), including dimethyl sulfide and trimethyl sulfide, which inhibited the growth of pathogenic bacteria and fungi in in vitro VOC assays. In pot experiments, the fermentation broth of Streptomyces sp. AN090126 reduced tomato bacterial wilt caused by Ralstonia solanacearum, red pepper leaf spot caused by Xanthomonas euvesicatoria, and creeping bentgrass dollar spot caused by Sclerotinia homoeocarpa in a dose-dependent manner. Moreover, the secondary metabolites derived from this strain showed a synergistic effect with streptomycin sulfate against streptomycin-resistant Pectobacterium carotovorum subsp. carotovorum, the causative agent of Kimchi cabbage soft rot, in both in vitro and in vivo experiments. Therefore, Streptomyces sp. AN090126 is a potential biocontrol agent in controlling plant diseases caused by pathogenic bacteria and fungi, specifically by the streptomycin-resistant strains.
APA, Harvard, Vancouver, ISO, and other styles
7

Kang, Seung-Hoon, Jianqiang Huang, Han-Na Lee, Yoon-Ah Hur, Stanley N. Cohen, and Eung-Soo Kim. "Interspecies DNA Microarray Analysis Identifies WblA as a Pleiotropic Down-Regulator of Antibiotic Biosynthesis in Streptomyces." Journal of Bacteriology 189, no. 11 (April 6, 2007): 4315–19. http://dx.doi.org/10.1128/jb.01789-06.

Full text
Abstract:
ABSTRACT Using Streptomyces coelicolor microarrays to discover regulators of gene expression in other Streptomyces species, we identified wblA, a whiB-like gene encoding a putative transcription factor, as a down-regulator of doxorubicin biosynthesis in Streptomyces peucetius. Further analysis revealed that wblA functions pleiotropically to control antibiotic production and morphological differentiation in streptomycetes. Our results reveal a novel biological role for wblA and show the utility of interspecies microarray analysis for the investigation of streptomycete gene expression.
APA, Harvard, Vancouver, ISO, and other styles
8

Rezzonico, Fabio, Virginia O. Stockwell, and Brion Duffy. "Plant Agricultural Streptomycin Formulations Do Not Carry Antibiotic Resistance Genes." Antimicrobial Agents and Chemotherapy 53, no. 7 (May 4, 2009): 3173–77. http://dx.doi.org/10.1128/aac.00036-09.

Full text
Abstract:
ABSTRACT Streptomycin is used in plant agriculture for bacterial disease control, particularly against fire blight in pome fruit orchards. Concerns that this may increase environmental antibiotic resistance have led to bans or restrictions on use. Experience with antibiotic use in animal feeds raises the possible influence of formulation-delivered resistance genes. We demonstrate that agricultural streptomycin formulations do not carry producer organism resistance genes. By using an optimized extraction procedure, Streptomyces 16S rRNA genes and the streptomycin resistance gene strA were not detected in agricultural streptomycin formulations. This diminishes the likelihood for one potential factor in resistance development due to streptomycin use.
APA, Harvard, Vancouver, ISO, and other styles
9

Ramón-García, Santiago, Isabel Otal, Carlos Martín, Rafael Gómez-Lus, and José A. Aínsa. "Novel Streptomycin Resistance Gene from Mycobacterium fortuitum." Antimicrobial Agents and Chemotherapy 50, no. 11 (September 5, 2006): 3920–22. http://dx.doi.org/10.1128/aac.00223-06.

Full text
Abstract:
ABSTRACT We have isolated the aph(3")-Ic gene, encoding an aminoglycoside 3"-O-phosphotransferase [APH(3")-Ic], from a genomic library of an environmental Mycobacterium fortuitum strain, selecting for streptomycin resistance. APH(3")-Ic phosphorylates and inactivates streptomycin. Similar genes have been described in Streptomyces griseus and plasmid RSF1010. It is also present in some M. fortuitum clinical isolates.
APA, Harvard, Vancouver, ISO, and other styles
10

BAUMANN, R., R. HÜTTER, and D. A. HOPWOOD. "Genetic Analysis in a Melanin-producing Streptomycete, Streptomyces glaucescens." Microbiology 81, no. 2 (February 1, 2000): 463–74. http://dx.doi.org/10.1099/00221287-81-2-463.

Full text
Abstract:
Summary: Using crossing and analysis procedures similar to those applied to Streptomyces coelicolor a3(2), several auxotrophic and streptomycin-resistant markers were located on a circular linkage map of the melanin-producing Streptomyces glaucescens, strain eth22794. The linkage map of S. glaucescens is similar to that of S. coelicolor ≪g≫a≪/g≫3(2), in the sequence of markers and in the presence of two long silent regions.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Streptomyce"

1

Meanwell, Richard J. L. "Streptomycin production from chitin using Streptomyces griseus." Thesis, Loughborough University, 2004. https://dspace.lboro.ac.uk/2134/12949.

Full text
Abstract:
The production of streptomycin using Streptomyces griseus using two types of chitin as a substrate was studied using a variety of fermentation techniques. Commercial chitin was obtained (Sigma) and comprised chemically purified crab shell. Pre-fermented chitin was the solid product from the lactic acid fermentation of shrimp waste using Lactobacillus paracasei A3. Bioassay, HPLC and FTIR methods were developed during this project for the quantification of streptomycin both in liquid phase and adsorbed on solid chitin surfaces. Shake flask experiments were carried out to determine basic production kinetics, as well as to establish if commercial and pre-fermented chitins produced different quantities of streptomycin. Shake flasks were also used to evaluate any effect of chitin concentration on streptomycin production. A range of submerged fermentations were undertaken in a standard 2 L bioreactor fitted with Rushton Turbines, at chitin concentrations from 0.4 %w/v to 10 %w/v, to study the effect on streptomycin yield. At concentrations of 5 %w/v and over, it was necessary to use an alternative, V-shaped agitator, as the Rushton Turbines did not provide adequate mixing. The V-shaped agitator was designed and produced as part of this project. The submerged fermenter was also used to determine if the re-use of .chitin remaining post-fermentation was possible. A solid state fermentation packed bed bioreactor was also developed, with a recycle loop for produced liquor. Four experiments evaluated the use of commercial and pre-fermented chitins, and different liquid media used for inoculation. In order to encompass the advantages of submerged and solid state fermentations, a vertical basket reactor was designed and manufactured, which used gentle fluidisation for the agitation of chitin particles contained inside the basket.Shake flask experimentation showed that pre-fermented chitin produced approximately 3 times the streptomycin yield than that of commercial chitin. Both systems reached a maximum liquid phase yield after 8 days of fermentation. Maximum streptomycin yields were obtained at a chitin concentration of 10 %w/v. The total streptomycin yields from submerged fermentation were fairly consistent over the range of chitin concentrations used. The amount of streptomycin adsorbed on the chitin surface, however, increased with increasing chitin concentration. Total streptomycin yields varied from 2 to 3.5 mglL. The re-use of chitin remaining post-fermentation was found to be possible in two series of three experiments. In both cases (at approximately 7.5 %w/v and 10 %w/v chitin) the lag phase and time to reach maximum biomass concentration decreased. Particle size analysis and mathematical modelling suggest that this is due to increasing specific surface of chitin particles during the course of fermentation. Both shake flask and submerged fermentation showed a bioassay inhibition peak in the tropophase, removable using 2 kDa membrane filters. Although it was not possible to determine the exact nature of the inhibiting component(s), streptomycin was eliminated through FTIR. A study of chitosan oligomers showed that short chain oligosaccharides inhibit Bacillus subtilis in a similar manner to streptomycin. Solid state fermentation using a salts solution liquid medium, with intermittent aeration and recirculation proved to be the most effective, giving a streptomycin yield of 3.8 mg/L. The vertical basket reactor obtained higher streptomycin yields of 4.6 mg/L. Post-fermentation washing with pH 3 buffer was also successfully used in this fermenter for the in-situ extraction of streptomycin, before the addition of fresh sterile liquid medium and fermentation re-start.
APA, Harvard, Vancouver, ISO, and other styles
2

Chen, X. "TAGGING BIOCONTROL STREPTOMYCES TO STUDY LETTUCE COLONIZATION." Doctoral thesis, Università degli Studi di Milano, 2015. http://hdl.handle.net/2434/345187.

Full text
Abstract:
The ability of the biological control agents (BCAs) to colonize plant tissues is an important feature involved in microbe-assisted plant protection. Plant-microbe interaction research increased especially in the last decade thanks to technological revolution. Molecular methods and the development of advanced microscopic techniques allow researchers to explore gene expression and localization of beneficial microorganisms within plants. The green fluorescent protein (GFP) and its modified version, enhanced GFP (EGFP), more adapt for expression in mammalian cells and GC-rich actinomycetes like Streptomyces, have been widely used as markers to study gene expression, as well as plant-microbe interactions. Aside fluorescent protein approaches, fluorescence in situ hybridization (FISH) is another frequently used technique to visualize microbial colonization patterns and community composition by application of specific fluorescent probes. Firstly, we transformed five Streptomyces strains, which showed strong inhibition activity against Sclerotinia sclerotiorum, with the EGFP construct by the conjugation method. The conjugation efficiencies varied between the strains, but were comparable to the reference strain. The fitness of transformed strains was similar to wild-type; the transformants maintained similar sporulation, mycelium growth rate, and the ability to produce important secondary metabolites and lytic enzymes. Secondly, two transformed strains, Streptomyces cyaneus ZEA17I, and Streptomyces sp. SW06W, were used to study lettuce colonization dynamics by seed coating method. Their spatio-temporal dynamics were determined in sterile substrate. The strains were consistently recovered from lettuce rhizosphere and inner root tissues up to six weeks. Finally, the colonization pattern of lettuce by Streptomyces cyaneus ZEA17I was examined by both EGFP and FISH approaches combined with confocal laser scanning microscopy (CLSM). For FISH-CLSM analysis, universal bacteria and Streptomyces genus specific probes were used to label S. cyaneus ZEA17I. The consistent presence of the labeled strain at the lettuce root one week after sowing showed that Streptomyces spores could rapidly germinate and produce filamentous mycelium on lettuce. S. cyaneus ZEA17I was detected also on two-week-old roots, indicating the long-term survival ability of this strain in lettuce rhizosphere. Altogether, the antagonistic activity, rhizosphere and root competence showed by the Streptomyces conferred their potential to act as BCA. Further studies on the complex host-pathogen-antagonist interactions will provide additional knowledge to understand the modes and mechanisms of Streptomyces-mediated plant protection.
APA, Harvard, Vancouver, ISO, and other styles
3

COLOMBO, ELENA MARIA. "EXPLORING STREPTOMYCES-FUSARIUM INTERACTION TO HAMPER WHEAT HEAD BLIGHT, CROWN ROT AND DEOXYNIVALENOL PRODUCTION." Doctoral thesis, Università degli Studi di Milano, 2019. http://hdl.handle.net/2434/692195.

Full text
Abstract:
La fusariosi della spiga e il marciume al colletto in frumento sono malattie causate da Fusarium graminearum, F. culmorum e F. pseudograminearum. Essi determinano ingenti perdite di raccolto oltre a contaminare il prodotto con micotossine appartenenti alla famiglia dei tricoteceni. I batteri Gram-positivi appartenenti al genere Streptomyces sono ubiquitari nel suolo ed endofiti dei tessuti interni delle radici. Essi producono una vasta gamma di metaboliti secondari con proprietà antimicrobiche e possono essere utilizzati come agenti promotori della crescita delle piante, limitando lo sviluppo dei patogeni. L’obiettivo del presente dottorato di ricerca è stato quello di selezionare ceppi di streptomiceti attivi contro Fusarium spp. in grano. La prima fase dello studio ha permesso di conoscere lo stato dell’arte sull’utilizzo di streptomiceti contro specie micotossigene appartenenti al genere Fusarium (Chapter 1). Successivamente sono state sviluppate strategie innovative per la selezione degli stessi (Chapter 2) e l’efficacia dei ceppi più promettenti è stata poi saggiata in diverse condizioni (Chapter 3). Inoltre, i metaboliti secondari responsabili dell’attività antifungina sono stati caratterizzati (Chapter 4). L’influenza della variabilità dei ceppi di Fusarium spp. e dei substrati colturali sull’attività di biocontrollo è stata valutata tramite saggi di antibiosi. Questi fattori hanno avuto un’influenza significativa nel determinare il livello di attività in vitro. I mezzi standard utilizzati in laboratorio hanno diminuito infatti tale parametro. Inoltre è stata riscontrata un’assenza di correlazione con il livello di biocontrollo ottenuto in pianta. Unica eccezione è per i risultati ottenuti utilizzando un terreno a base di frumento, che ha permesso di osservare un valore di correlazione più elevato con il livello di biocontrollo riscontrato contro marciume radicale in frumento (r = 0.5). Successivamente, al fine di saggiare metaboliti limitanti la produzione di tricoteceni, è stato sviluppato un sistema in micropiastra che misura la fluorescenza emessa da un ceppo di F. graminearum trasformato con il costrutto TRI5::GFP. TRI5 è il primo gene essenziale coinvolto nella via metabolica di produzione di tricoteceni. Da questa prova si è potuto selezionare il ceppo di streptomicete DEF39 che riduce significativamente la produzione di DON. Le potenziali attività di promozione della crescita e di biocontrollo di una selezione dei ceppi più promettenti (N = 21) sono state saggiate in pianta. Gli streptomiceti testati non hanno esibito la capacità di aumentare i parametri di sviluppo dei germinelli di frumento, ma il ceppo DEF09 ha ridotto significativamente il marciume al colletto e la fusariosi della spiga ottenendo livelli di protezioni sopra al 40% e del 60% rispettivamente. Basandosi sui risultati ottenuti, quattro ceppi (DEF09, DEF20, DEF39, DEF48) sono stati applicati su grano sterilizzato testando due tempistiche di trattamento per osservarne la capacità di riduzione della biomassa fungina e della produzione di DON. Inoltre tramite qPCR si è osservata la fitness degli agenti di biocontrollo nelle condizioni testate. Gli streptomiceti, abili colonizzatori del substrato testato, sono stati efficaci nel ridurre la produzione di micotossine, limitando -quando co-inoculati con il patogeno- lo sviluppo dello stesso. I ceppi selezionati agiscono perciò sia contro lo sviluppo fugino e/o contro la produzione di DON. Ulteriori studi saranno necessari per confermarne l’attività in pianta, così come per permettere lo sviluppo di formulati efficaci per limitare la contaminazione da tricoteceni.
Fusarium head blight (FHB), root rot (FRR) and foot rot (FFR) cause important yield losses in wheat. The harvested product is often contaminated with mycotoxins, belonging to the group of trichothecenes. The main causal agents are Fusarium graminearum, F. culmorum and F. pseudograminearum. The biocontrol approach is a feasible option in order to reduce disease severity, as well as trichothecene contamination in grains. Streptomyces spp. are Gram-positive bacteria, ubiquitous in soil and endophytes of inner tissues of plant roots. They produce a wide range of secondary metabolites able to limit pathogen development and disease severity in planta, as well as to enhance plant growth. This PhD project aimed to select Streptomyces strains active within the wheat-Fusarium spp. pathosystem. To achieve this, a detailed literature and patents analysis focused on biocontrol of toxigenic Fusarium spp. was carried out (Chapter 1) and new methodological approaches for antagonist screening have been developed (Chapter 2). Furthermore, the biocontrol efficacy of a selected subset of strains obtained from the culture collection maintained at the Plant Pathology laboratory (DeFENS, University of Milan, Italy) was evaluated in different conditions (Chapter 3) and bioactive metabolites were isolated (Chapter 4). The influence of growth media and Fusarium strain diversity on streptomycete antifungal activity was assessed in dual culture assays. All the factors influenced the level of antifungal activity. The media commonly used for in vitro screening reduced the inhibitory activity of streptomycetes. Overall, results from dual culture assays and level of disease protection observed in planta did not correlate, except for those recorded on a medium based on wheat grains. Indeed, it was the most effective in eliciting antifungal activity and showed the highest correlation (r = 0.5) with FRR inhibition. Subsequently, being TRI5 the first and essential gene involved in trichothecene biosynthetic pathway in Fusarium spp., a microplate bioassay using a TRI5::GFP transformed F. graminearum strain was developed and validated in order to screen the effect of natural products on GFP fluorescence and consequently on trichothecene production. Surprisingly, culture filtrate from DEF39 strain completely suppressed deoxynivalenol (DON) production without affecting fungal growth. The most promising isolates (N = 21) were further characterized for their potential plant growth promotion ability, as well as for their activity against FRR and FFR in wheat seedlings. None of them was able to increase plant growth. However, DEF09 strain exhibited consistent efficacy to limit FRR-FFR symptom severity (protection level > 40%) in soil and soilless conditions. Therefore, a field trial was performed to test its ability to reduce FHB severity, obtaining up to 60% protection. Based on the activity observed from the previous screenings, four promising streptomycetes (DEF09, DEF20, DEF39, DEF48) were applied on sterilized wheat grains (microsilage) at two timepoints of application, in order to evaluate their ability to suppress fungal growth and DON production. Moreover, the fitness of streptomycetes in microsilage conditions was assessed by qPCR analysis. Streptomycetes were able to efficiently colonize the substrate, which resulted in reducing fungal biomass and DON accumulation only when co-inoculated with the pathogen. A pool of promising biocontrol agents has been selected against fungal development and/or DON production. This research highlighted the complexity of finding an efficient screening procedure due to multiple interactions occurring in wheat-Fusarium spp. pathosystem. Further studies will be needed to confirm the activity of the strains in planta. The identification of the mechanisms of action and the molecules involved in the bioactivity of the strains will possibly allow to develop effective treatments limiting trichothecene accumulation in wheat.
APA, Harvard, Vancouver, ISO, and other styles
4

Egan, Sharon. "Analysis of the distribution and diversity of streptomycin biosynthetic and resistance genes in populations of Streptomyces." Thesis, University of Warwick, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343828.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Coyne, Vernon Errol. "Genetic studies of Streptomyces cattleya and Streptomyces olivaceus." Doctoral thesis, University of Cape Town, 1985. http://hdl.handle.net/11427/17599.

Full text
Abstract:
Bibliography: pages 243-259.
Actinophage VCll is able to virulently infect 11 of the 20 Streptomyces strains tested. Examination of VCll infection of Streptomyces cattleya, Streptomyces olivaceus and Streptomyces lividans TC10 indicated the absence of restriction-modification systems which affect VCll infectivity of these Streptomyces strains.
APA, Harvard, Vancouver, ISO, and other styles
6

Thamm, Sven. "Genetische und biochemische Untersuchungen von StrR dem "pathway"-spezifischen Transkriptionsaktivator von Genen der Streptomycin-Biosynthese in Streptomyces griseus N2-3-11 /." [S.l. : s.n.], 1999. http://deposit.ddb.de/cgi-bin/dokserv?idn=975452363.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Genay, Magali Decaris Bernard Dary Annie. "Impact de la réponse stringente sur la mutabilité de Streptomyces coelicolor et Streptomyces ambofaciens au cours de la différenciation." [S.l.] : [s.n.], 2006. http://www.scd.uhp-nancy.fr/docnum/SCD_T_2006_0123_GENAY.pdf.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Murad, Fatima. "Etude de la résistance aux macrolides chez Streptomyces ambofaciens et Streptomyces lividans." Paris 11, 2003. http://www.theses.fr/2003PA112172.

Full text
Abstract:
Les antibiotiques macrolides inhibent la synthèse protéique en se liant aux ribosomes. Streptomyces ambofaciens produit la spiramycine et possède plusieurs mécanismes de résistance à la spiramycine et aux macrolides. J'ai caractérisé, le déterminant de résistance srmC et montré qu'il était constitué des trois gènes srmC1, srmC2 et srmC3. SrmC1 et srmC2 codent les sous-unités d'un transporteur de la famille ABC (ATP Binding Cassette) et confèrent la résistance à certains macrolides, dont la spiramycine, mais aussi à la daunorubicine. SrmC1/C2 sont co-transcrits et leur expression est réprimée par SrmC3, un répresseur transcriptionnel de la famille TetR. Les gènes srmC ne sont pas localisés parmi les gènes, de biosynthèse de la spiramycine et ne sont pas indispensables chez S. Ambofaciens. En étudiant chez Streptomyces lividans le mécanisme d'induction de l'expression de srmC1/C2, j'ai mis en évidence l'existence dans cette souche d'un nouveau mécanisme de résistance aux macrolides et à d'autres antibiotiques, inductible par un macrolide, la rosaramicine. Des orthologues de srmC1/C2, désignés sclA et sclB, codant les sous-unités d'un transporteur ABC, sont impliqués dans cette résistance. Ils confèrent une résistance multi-drogue (macrolides, daunorubicine, bromure d'éthidium). Leur expression est réprimée par SclR (répresseur transcriptionnel de la famille TetR. ). L'étude d'un mutant SclA ̄ SclR ̄suggère qu'un ou plusieurs autres déterminants participent également à la résistance multi-drogue inductible par la rosaramicine. Parmi les gènes de résistance aux macrolides de S. Ambofaciens, certains ont des orthologues chez S. Lividans, un non-producteur de macrolide, d'autres non. Ceci suggère que certains déterminants de résistance de S. Ambofaciens sont liés à la biosynthèse de spiramycine et jouent un rôle crucial dans la protection de la souche. D'autres, dont srmC, protégeraient la souche contre des produits toxiques présents dans l'environnement
Macrolide antibiotics inhibit protein synthesis by binding to ribosomes. Streptomyces ambofaciens produces spiramycin and possesses several resistance mechanisms to spiramycine and other macrolides. The srmC determinant was studied in the work reported here. It is comprised of three genes: srmC1, srmC2 and srmC3. SrmC1/srmC2 encode the two sub-units of an ABC (ATP Binding Cassette) transporter. They confer resistance to several macrolides, including spiramycin, and to daunorubicin. The two genes srmC1/srmC2 are co-transcribed and their expression is repressed by SrmC3, a repressor of the TetR family. The srmC genes are not localized in the spiramycin biosynthetic gene cluster and are dispensable in S. Ambofaciens. During the study of the induction of srmCl/C2 expression in S. Lividans, a new mechanism of resistance to macrolides and other antibiotics, inducible by the macrolide rosaramicin was discovered in this strain. Genes sclA and sclB, orthologous to srmC1/C2, encoding an ABC transporter are involved in this resistance. SclA and sclB confer multidrug resistance (resistance to macrolides daunorubicin and ethidium bromide). Their expression is controlled by SclR (a TetR-like repressor). The study of a SclA ̄SclR ̄mutant strain suggested that other resistance determinant(s) might be involved in the rosaramicin-inducible resistance. Among macrolide resistance genes from S. Ambofaciens, some have homologues in S. Lividans, others do not. This suggests that some S. Ambofaciens resistant determinants could be directly linked to spiramycin biosynthesis and important for self-protection against spiramycin. Others, including srmC might protect the strain against other toxic compounds encountered in the environment
APA, Harvard, Vancouver, ISO, and other styles
9

Carter, T. P. "Keratinase from Streptomyces fradiae." Thesis, Cranfield University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280938.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Calcutt, Michael John. "Pactamycin resistance in Streptomyces." Thesis, University of Leicester, 1987. http://hdl.handle.net/2381/35191.

Full text
Abstract:
The coupled transcription-translation system previously developed for Streptomyces lividans was modified such that it functioned using purified ribosomal subunits, a crude initiation factor preparation and a high speed supernatant fraction. This system was used to investigate antibiotic resistance mechanisms in two Streptomyces which synthesise inhibitors of translation. Resistance to either pactamycin in Streptomyces pactum or celesticetin in Streptomyces caelestis was due to ribosome modification. In each case, high level resistance was attributed solely to one ribosomal subunit, the 30S subunit of the S. pactum ribosome and the 50S subunit of the S. caelestis ribosome. Shotgun cloning experiments have enabled a pactamycin resistance determinant from S. pactum to be isolated in S. lividans. However, in the original pactamycin resistant clone the plasmid was unstable and in the absence of pactamycin selection pressure, only a deleted form could be recovered. When ribosomes from resistant subclones were analysed, it appeared that a ribosome modification system from S. pactum had been cloned. Ribosome reconstitution studies indicated that a property of 16S rRNA was responsible for resistance. Since the cloned resistance determinant was not homologous to 16S rRNA (as judged by Southern analysis), pactamycin resistance in S. pactum is probably due to post-transcriptional modification of 16S rRNA.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Streptomyce"

1

Frössén, Gunnar. Streptomyces griseus. Stockholm: Carlsson, 2007.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

Egan, Sharon. Analysis of the distribution and diversity of streptomycin biosynthetic and resistance genes in populations of Streptomyces. [s.l.]: typescript, 1999.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

W, Queener Stephen, and Day Lawrence E, eds. Antibiotic-producing streptomyces. Orlando, FL: Academic Press, 1986.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

Hōsenkin to ikiru: Nihon Hōsenkin Gakkai 25-shūnen kinen. Tōkyō: Mimizukusha, 2011.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Rojas, A. E. Carvajal de. Carbon catabolism in streptomyces venezuelae. Manchester: UMIST, 1995.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

Baumberg, Simon, Hans Krügel, and Dieter Noack, eds. Genetics and Product Formation in Streptomyces. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5922-7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

S, Baumberg, Krügel Hans, Noack Dieter, and Federation of European Microbiological Societies., eds. Genetics and product formation in streptomyces. New York: Plenum Press, 1991.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

Paget, M. S. B. Gene regulation and expression vector developmentin streptomyces. Manchester: UMIST, 1994.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

Leigh, Michael James. Peptide transport in streptomyces coelicolor A3(2). [s.l.]: typescript, 1992.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Keller, Birgit. Photobiologische Untersuchungen an Sporen von Streptomyces griseus. Koln: Deutsche Forschungsanstalt fur Luft- und Raumfahrt, 1991.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Streptomyce"

1

Chater, K. F., and D. A. Hopwood. "Streptomyces." In Bacillus subtilis and Other Gram-Positive Bacteria, 83–99. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555818388.ch6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Gooch, Jan W. "Streptomycin." In Encyclopedic Dictionary of Polymers, 926. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14873.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

de Groot, Anton C. "Streptomycin." In Monographs In Contact Allergy, 886–89. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003158004-453.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Kado, Clarence I., and Brian Kelly. "Actinomycetes (Streptomyces lividans)." In Agrobacterium Protocols Volume 2, 395–401. Totowa, NJ: Humana Press, 2006. http://dx.doi.org/10.1385/1-59745-131-2:395.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Jauri, Patricia Vaz, Nora Altier, and Linda L. Kinkel. "Streptomyces for Sustainability." In Microbial Models: From Environmental to Industrial Sustainability, 251–76. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-2555-6_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Kollárová, M., E. Kašová, K. Szuttorová, H. Follmann, and J. Zelinka. "Thioredoxin from Streptomyces Aureofaciens." In Metabolism and Enzymology of Nucleic Acids, 37–41. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-0749-5_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Hopwood, David A., and Tobias Kieser. "Conjugative Plasmids of Streptomyces." In Bacterial Conjugation, 293–311. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4757-9357-4_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Schrempf, H. "Genetic Instability in Streptomyces." In Genetics and Product Formation in Streptomyces, 245–52. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5922-7_29.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Elliot, Marie A., Mark J. Buttner, and Justin R. Nodwell. "Multicellular Development in Streptomyces." In Myxobacteria, 419–38. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815677.ch24.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Schomburg, Dietmar, and Margit Salzmann. "Streptomycin-6-phosphatase." In Enzyme Handbook 3, 473–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76463-9_100.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Streptomyce"

1

Setyawati, Tri Rima, Rikhsan Kurniatuhadi, and Ari Hepi Yanti. "Karakter Morfologi Koloni Streptomyces Spp. yang diisolasi dari Substrat Habitat Cacing Nipah (Namalycastis Rhodochorde) pada Medium Berbeda." In Seminar Nasional Penerapan Ilmu Pengetahuan dan Teknologi : kampus merdeka meningkatkan kecerdasan sumberdaya manusia melalui interdispliner ilmu pengetahuan dan teknologi : Pontianak, 24 Agustus 2021. Untan Press, 2021. http://dx.doi.org/10.26418/pipt.2021.29.

Full text
Abstract:
Streptomyces memiliki potensi sebagai sumber metabolit antibakteri yang dapat dikembangkan dan diaplikasikan dalam pengembangan budidaya cacing nipah. Streptomyces memiliki karakter morfologi kultur yang berbeda-beda di beberapa medium agar dan merupakan aspek yang sangat penting dalam proses identifikasi dan klasifikasinya. Streptomyces spp. telah diisolasi dari substrat alami habitat cacing nipah (Namalycastis rhodochorde) di Sungai Kakap Kabupaten Kubu Raya sebanyak enam isolat (NrASA1 – NrASA6) dan telah berhasil dimurnikan untuk dilakukan karakterisasi morfologi koloninya. Penelitian ini bertujuan untuk mengkarakterisasi variasi koloni Streptomyces sehingga diketahui variasi koloni dari genus yang sama pada medium yang berbeda. Karakterisasi dilakukan dengan metode pengamatan langsung terhadap koloni Streptomyces yang dikultur secara kuadran pada medium agar glycerol asparagine (GAA), inorganic salt starch (ISSA), oatmeal (OA), dan starch casein (SCA). Hasil karakterisasi tujuh isolat Streptomyces spp. yang dikultur pada empat medium agar berbeda memperlihatkan bahwa persamaan karakter terlihat dari warna spora matang yang 100% berwarna coklat, interval diameter koloni antara 3 – 8 cm, tekstur bersifat cottony. Perbedaan karakter terlihat dari presensi diffusible pigment yang hanya ada pada koloni isolat NrASA1 dan NrASA3 pada medium GAA dan SCA, sedangkan presensi eksudat berwarna coklat tua hanya terjadi pada medium ISSA. Enam isolat Streptomyces diduga memiliki epitet spesies yang berbeda, khususnya antara NrASA1 dan NrASA3 terhadap kode isolat lainnya.
APA, Harvard, Vancouver, ISO, and other styles
2

Loboda, M., and L. Biliavska. "Biosynthesis of polyene antibiotics and phytohormones under the action of exogenous β-sitosterol by soil Streptomycete Streptomyces Netropsis IMV Ac-5025." In National Scientific Symposium With International Participation: Modern Biotechnologies – Solutions to the Challenges of the Contemporary World. Institute of Microbiology and Biotechnology, Republic of Moldova, 2021. http://dx.doi.org/10.52757/imb21.085.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Nazarova, Ya I., A. V. Bakulina, I. G. Shirokikh, and A. L. Blinova. "Studying the properties of rhizosphere strain Streptomyces sp. 8Al3 for phytopathogens biocontrol." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.180.

Full text
Abstract:
The species identification of the rhizosphere strain Streptomyces sp. 8Al3 was done, studied its antagonistic properties, the ability to produce phytohormones, and optimized the cultivation conditions.
APA, Harvard, Vancouver, ISO, and other styles
4

Tovstik, E. V., and A. V. Bakulina. "Search for streptomycetes with antagonistic activity to the aggressive Fusarium sp. strain AC." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.251.

Full text
Abstract:
The antagonistic activity of 126 Streptomyces strains against the fungus Fusarium sp. AC was studied. The greatest extent of mycelium growth was inhibited by S. geldanamicininus 3K9, which produces validamycin A.
APA, Harvard, Vancouver, ISO, and other styles
5

Luo, Jianmei, Jianshu Li, Yanting Wang, Shenheng Luo, and Min Wang. "Protoplast Formation and Regeneration Conditions of Streptomyces gilvosporeus." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5163260.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Thomas, Spencer Angus, Yaochu Jin, Emma Laing, and Colin P. Smith. "Reconstructing regulatory networks in Streptomyces using evolutionary algorithms." In 2013 13th UK Workshop on Computational Intelligence (UKCI). IEEE, 2013. http://dx.doi.org/10.1109/ukci.2013.6651283.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

T R, Akshaya, Dhevendaran K, and Ravikumar R. "Isolation and characterization of carotenoids from Streptomyces spp." In 3rd Annual International Conference on Advances in Biotechnology (BioTech 2013). Global Science and Technology Forum, 2013. http://dx.doi.org/10.5176/2251-2489_biotech13.27.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Indrakumar, Janani, and Kandhasamy Dhevendaran. "immunostimulant and antimicrobial properties of selected Streptomyces spp." In 3rd Annual International Conference on Advances in Biotechnology (BioTech 2013). Global Science and Technology Forum, 2013. http://dx.doi.org/10.5176/2251-2489_biotech13.44.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Liu, Hua-zhen, Wei Chen, Qi-ying Liu, Xia Zhang, Li-xiu Wang, and Cheng-wu Chi. "A NEW PEPTIDE THROMBIN INHIBITOR FROM STREPTOMYCES GRISEUS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644330.

Full text
Abstract:
A new peptide thrombin inhibitor was found in the Streptomyces griseus strain 254 isolated from a soil sample from Tongan, Fujian province, China, the inhibitor being a secondary metabolic product. The production of the inhibitor reached a maximum after 3 days culture of bacteria at 28°C in a rotary shaker. The inhibitor excreted in the culture filtrate was purified by absorption on macroporous resin, followed by ion exchange chromatography on DEAE-52, CM-32 cellulose, affinity chromatography on the immobilized thrombin and high performance liquid chromatography. The amino acid composition of the inhibitor was determined to be Val(2), Met(l), Ile(l), Leu(2) and Arg (1), similar to that of the amino acid residues around the reactive site of human antithrombin III, the critical plasma inhibitor of thrombin. The NH2-terminal residue of the inhibitor seems to be blocked by the alkyl group due to the negative reaction to ninhydrin, whereas the COO-terminal residue is most likely to be arginal because of that Arg was not found in the amino acid analysis, unless the peptide was oxidized by performic acid before acid hydrolysis. The chromogen substrates Bz-Phe-Val-Arg-PNA and Bz-Gly-Pro-Lys-PNA were used to determine the thrombin and plasmin activities, respectively. Besides thrombin, the purified inhibitor also exhibits a weak inhibitory activities on trypsin and much weak on plasmin, but not on chymotrypsin and other protein-ases.
APA, Harvard, Vancouver, ISO, and other styles
10

Granato, Ana Claudia, Luis H. Romano, Jaine H. H. L. Oliveira, Regiane P. Ratti, Isara L. C. Hernandez, Raquel C. Montenegro, Marlei Barboza, Cristina P. Souza, Carlos O. Hokka, and Milan Trsic. "Chemical and pharmacological study of Brazilian marine Streptomyces." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0101.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "Streptomyce"

1

Crawford, D. L. Genetics and chemistry of lignin degradation by Streptomyces. Office of Scientific and Technical Information (OSTI), January 1992. http://dx.doi.org/10.2172/6643947.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Crawford, D. L. Genetics and chemistry of lignin degradation by Streptomyces. Final technical report. Office of Scientific and Technical Information (OSTI), December 1992. http://dx.doi.org/10.2172/10140506.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Barash, Isaac, Rosemary Loria, Giora Kritzman, Shulamit Manulis, and Yair Aharonowitz. Application of Recombinant DNA and Immunological Technologies for Development of Diagnostic Tools for Phytopathogenic Streptomyces Spp. United States Department of Agriculture, December 1990. http://dx.doi.org/10.32747/1990.7603799.bard.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Adam, Zach, and Eran Pichersky. Degradation of Abnormal Proteins in Chloroplasts of Higher Plants. United States Department of Agriculture, August 1994. http://dx.doi.org/10.32747/1994.7568768.bard.

Full text
Abstract:
In this study we attempted to get a better understanding of processes involved in the degradation of abnormal proteins i chloroplasts. To achieve this goal, we used a number of complementary approaches. We first characterized the expression of the two subunits of Clp protease. We demonstrated that both of them were expressed in chloroplasts in a constitutive fashion, but the expression of the regulatory subunit ClpC was enhanced by light. We generated a mutant the lumenal protein OEE33 which was targeted to the stroma in in vitro experiments. In the wrong compartment it was found unstable, and characterization of its degradation revealed that it was degraded by a soluble, ATP-dependent serine protease, which are also the characteristics of Clp protease. In search of other homologues of bacterial proteases, we found that chloroplasts contain a homologue of the FtsH protease. It is an ATP-dependent metallo-protease, bound to the stromal side of the thylakoid membrane, whose expression is dependent on light. The gene encodig this protease was cloned and characterized. In attempt to generate Arabidopsis mutant plants impaired in their capability to degrade abnormal chloroplast proteins, we fused the gene for mistargeted OEE33 to the streptomycin-detoxifying gene. This chimeric gene was introduced into Arabodipsis plants, to generate transformed plants. This transformants plants were sensitive to streptomycin due to the rapid turn-over of the chimeric protein. Seeds from these plants were then chemically mutagenised, and seedlings were selected for their capability to grow on streptomycin. The ability of these mutant transformants to grow on streptomycin is presumably due to stabilization of the chimeric protein. These plants will allow us in the future to identify the effected genes, which are likely to be involved in the protein degradation process.
APA, Harvard, Vancouver, ISO, and other styles
5

Singh, Renu. Enzymatic Control of the Related Pathways of Fatty Acid and Undecylprodiginine Biosynthesis in Streptomyces coelicolor. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.2110.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Lindow, Steven E., Shulamit Manulis, Dan Zutra, and Dan Gaash. Evaluation of Strategies and Implementation of Biological Control of Fire Blight. United States Department of Agriculture, July 1993. http://dx.doi.org/10.32747/1993.7568106.bard.

Full text
Abstract:
The main objective of this study was to develop data that would facilitate a consistently effective method of biological control of fire blight disease to be developed and to enable its implementation for disease control by ensuring its compatibility with variations in the biological, environmental, and chemical conditions present in pear orchards. As considerable information on the pathogen and biological control of fire blight was already gathered from studies in California and elsewhere, an emphasis was placed on investigating the genetics and ecology of Erwinia amylovora, the causal agent of fire blight in Israel. Studies of plasmid profile, virulence on several host, serological characteristics, as well as DNA fingerprints with selected primers all revealed E. amylovora strains in Israel to be homogeneous. Strains did vary in their resistance to streptomycin, with those from more northern locations being resistant while those in the southern costal plain were all sensitive to streptomycin. Resistance appeared to be conferred by chromosomal mutations as in streptomycin-resistant strains in California. The biological control agent Pseudomonas fluorescens strain A506 colonized flowers of both the Costia and Spodona pear cultivars in Israel as well as Bartlett pear in California. Flowers that were open at the time of spray inoculation of trees subsequently harbored from 105 to 107 cells of strain A506 per flower, while those that opened subsequent to spraying developed population sizes of about 105 cells/flower within 5 days. The incidence of fire blight infections were reduced about 3-fold in several trials in which moderate amounts of disease occurred in the plot areas; this degree of biological control is similar to that observed in California and elsewhere. On two occasions warm and moist weather that favored disease led to epidemics in which nearly all flowers became infected and which was so severe that neither P. fluorescens strain A506 nor chemical bactericides reduced disease incidence. A novel method for identifying antagonistic microorganisms for biological control of fire blight and other diseases was developed. A bacterial ice nucleation gene was introduced into E. amylovora to confer an Ice+ phenotype and the population sizes of this modified pathogen on flowers that had been pre-treated with potential control agents was estimated by measuring the freezing temperature of colonized flowers. Antagonistic strains that prevented the growth of E. amylovora in flowers were readily detected as those in which flowers froze at a low temperature. The method is both rapid and unbiased and several bacterial strains with substantial biological control potential have been identified using this method.
APA, Harvard, Vancouver, ISO, and other styles
7

Michel Jr., Frederick C., Harry A. J. Hoitink, Yitzhak Hadar, and Dror Minz. Microbial Communities Active in Soil-Induced Systemic Plant Disease Resistance. United States Department of Agriculture, January 2005. http://dx.doi.org/10.32747/2005.7586476.bard.

Full text
Abstract:
Induced Systemic Resistance (ISR) is a highly variable property that can be induced by compost amendment of potting media and soils. For example, previous studies showed that only 1 of 79 potting mixes prepared with different batches of mature composts produced from several different types of solid wastes were able to suppress the severity of bacterial leaf spot of radish caused by Xanthomonas campestris pv. armoraciae compared with disease on plants produced in a nonamended sphagnum peat mix. In this project, microbial consortia in the rhizosphere of plants grown in ISR-active compost-amended substrates were characterized. The plants used included primarily cucumber but also tomato and radish. Rhizosphere microbial consortia were characterized using multiple molecular tools including DGGE (Israel) and T -RFLP (Ohio) in both ISR-active field plots and potting media. Universal as well as population-specific bacterial and fungal PCR primers were utilized. T -RFLP analyses using universal bacterial primers showed few significant differences in overall bacterial community composition in ISR-active and inactive substrates (Ohio). In addition, the community members which were significantly different varied when different ISR-activecomposts were used (Ohio). To better characterize the shifts in microbial community structure during the development of ISR, population specific molecular tools were developed (Israel, Ohio).-PCR primers were designed to detect and quantify bacterial groups including Pyrenomycetes, Bacillus, Pan toea, Pseudomonas, Xanthomonas and Streptomyces as well as Trichoderma and Fusarium; two groups of fungi that harbor isolates which are ISR active (Isreal and Ohio). Bacterial consortia associated with cucumber plants grown in compost-amended potting mixtures were shown to be dominated by the phylogenetic taxon Bacteroidetes, including members of the genus Chryseobacterium, which in some cases have been shown to be involved in biocontrol (Israel). Nested-PCR-DGGE analyses coupled with long l6S rDNA sequencing, demonstrated that the Chryseobacteriumspp. detected on seed and the root in compost-amended treatments were derived from the compost itself. The most effective ISR inducing rhizobacterial strains were identified as Bacillus sp. based on partial sequencing of l6S rDNA. However, these strains were significantly less effective in reducing the severity of disease than Trichoderma hamatum382 (T382). A procedure was developed for inoculation of a compost-amended substrate with T -382 which consistently induced ISR in cucumber against Phytophthora blight caused by Phytophthora capsiciand in radish against bacterial spot (Ohio). Inoculation of compost-amended potting mixes with biocontrol agents such as T -382 and other microbes that induce systemic resistance in plants significantly increased the frequency of systemic disease control obtained with natural compost amendments.
APA, Harvard, Vancouver, ISO, and other styles
8

Jorgensen, Frieda, Andre Charlett, Craig Swift, Anais Painset, and Nicolae Corcionivoschi. A survey of the levels of Campylobacter spp. contamination and prevalence of selected antimicrobial resistance determinants in fresh whole UK-produced chilled chickens at retail sale (non-major retailers). Food Standards Agency, June 2021. http://dx.doi.org/10.46756/sci.fsa.xls618.

Full text
Abstract:
Campylobacter spp. are the most common bacterial cause of foodborne illness in the UK, with chicken considered to be the most important vehicle for this organism. The UK Food Standards Agency (FSA) agreed with industry to reduce Campylobacter spp. contamination in raw chicken and issued a target to reduce the prevalence of the most contaminated chickens (those with more than 1000 cfu per g chicken neck skin) to below 10 % at the end of the slaughter process, initially by 2016. To help monitor progress, a series of UK-wide surveys were undertaken to determine the levels of Campylobacter spp. on whole UK-produced, fresh chicken at retail sale in the UK. The data obtained for the first four years was reported in FSA projects FS241044 (2014/15) and FS102121 (2015 to 2018). The FSA has indicated that the retail proxy target for the percentage of highly contaminated raw whole retail chickens should be less than 7% and while continued monitoring has demonstrated a sustained decline for chickens from major retailer stores, chicken on sale in other stores have yet to meet this target. This report presents results from testing chickens from non-major retailer stores (only) in a fifth survey year from 2018 to 2019. In line with previous practise, samples were collected from stores distributed throughout the UK (in proportion to the population size of each country). Testing was performed by two laboratories - a Public Health England (PHE) laboratory or the Agri-Food & Biosciences Institute (AFBI), Belfast. Enumeration of Campylobacter spp. was performed using the ISO 10272-2 standard enumeration method applied with a detection limit of 10 colony forming units (cfu) per gram (g) of neck skin. Antimicrobial resistance (AMR) to selected antimicrobials in accordance with those advised in the EU harmonised monitoring protocol was predicted from genome sequence data in Campylobacter jejuni and Campylobacter coli isolates The percentage (10.8%) of fresh, whole chicken at retail sale in stores of smaller chains (for example, Iceland, McColl’s, Budgens, Nisa, Costcutter, One Stop), independents and butchers (collectively referred to as non-major retailer stores in this report) in the UK that are highly contaminated (at more than 1000 cfu per g) with Campylobacter spp. has decreased since the previous survey year but is still higher than that found in samples from major retailers. 8 whole fresh raw chickens from non-major retailer stores were collected from August 2018 to July 2019 (n = 1009). Campylobacter spp. were detected in 55.8% of the chicken skin samples obtained from non-major retailer shops, and 10.8% of the samples had counts above 1000 cfu per g chicken skin. Comparison among production plant approval codes showed significant differences of the percentages of chicken samples with more than 1000 cfu per g, ranging from 0% to 28.1%. The percentage of samples with more than 1000 cfu of Campylobacter spp. per g was significantly higher in the period May, June and July than in the period November to April. The percentage of highly contaminated samples was significantly higher for samples taken from larger compared to smaller chickens. There was no statistical difference in the percentage of highly contaminated samples between those obtained from chicken reared with access to range (for example, free-range and organic birds) and those reared under standard regime (for example, no access to range) but the small sample size for organic and to a lesser extent free-range chickens, may have limited the ability to detect important differences should they exist. Campylobacter species was determined for isolates from 93.4% of the positive samples. C. jejuni was isolated from the majority (72.6%) of samples while C. coli was identified in 22.1% of samples. A combination of both species was found in 5.3% of samples. C. coli was more frequently isolated from samples obtained from chicken reared with access to range in comparison to those reared as standard birds. C. jejuni was less prevalent during the summer months of June, July and August compared to the remaining months of the year. Resistance to ciprofloxacin (fluoroquinolone), erythromycin (macrolide), tetracycline, (tetracyclines), gentamicin and streptomycin (aminoglycosides) was predicted from WGS data by the detection of known antimicrobial resistance determinants. Resistance to ciprofloxacin was detected in 185 (51.7%) isolates of C. jejuni and 49 (42.1%) isolates of C. coli; while 220 (61.1%) isolates of C. jejuni and 73 (62.9%) isolates of C. coli isolates were resistant to tetracycline. Three C. coli (2.6%) but none of the C. jejuni isolates harboured 23S mutations predicting reduced susceptibility to erythromycin. Multidrug resistance (MDR), defined as harbouring genetic determinants for resistance to at least three unrelated antimicrobial classes, was found in 10 (8.6%) C. coli isolates but not in any C. jejuni isolates. Co-resistance to ciprofloxacin and erythromycin was predicted in 1.7% of C. coli isolates. 9 Overall, the percentages of isolates with genetic AMR determinants found in this study were similar to those reported in the previous survey year (August 2016 to July 2017) where testing was based on phenotypic break-point testing. Multi-drug resistance was similar to that found in the previous survey years. It is recommended that trends in AMR in Campylobacter spp. isolates from retail chickens continue to be monitored to realise any increasing resistance of concern, particulary to erythromycin (macrolide). Considering that the percentage of fresh, whole chicken from non-major retailer stores in the UK that are highly contaminated (at more than 1000 cfu per g) with Campylobacter spp. continues to be above that in samples from major retailers more action including consideration of interventions such as improved biosecurity and slaughterhouse measures is needed to achieve better control of Campylobacter spp. for this section of the industry. The FSA has indicated that the retail proxy target for the percentage of highly contaminated retail chickens should be less than 7% and while continued monitoring has demonstrated a sustained decline for chickens from major retailer stores, chicken on sale in other stores have yet to meet this target.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Streptomyce' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography