Relevant bibliographies by topics / Stop TMT / Journal articles

Journal articles on the topic 'Stop TMT'

To see the other types of publications on this topic, follow the link: Stop TMT.
Author: Grafiati
Published: 10 December 2022
Last updated: 29 January 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Stop TMT.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Zhang, J. T. "Sequence requirements for membrane assembly of polytopic membrane proteins: molecular dissection of the membrane insertion process and topogenesis of the human MDR3 P-glycoprotein." Molecular Biology of the Cell 7, no. 11 (November 1996): 1709–21. http://dx.doi.org/10.1091/mbc.7.11.1709.

Full text
Abstract:
The biogenesis of membrane proteins with a single transmembrane (TM) segment is well understood. However, understanding the biogenesis and membrane assembly of membrane proteins with multiple TM segments is still incomplete because of the complexity and diversity of polytopic membrane proteins. In an attempt to investigate further the biogenesis of polytopic membrane proteins, I used the human MDR3 P-glycoprotein (Pgp) as a model polytopic membrane protein and expressed it in a coupled cell-free translation/translocation system. I showed that the topogenesis of the C-terminal half MDR3 Pgp molecule is different from that of the N-terminal half. This observation is similar to that of the human MDR1 Pgp. The membrane insertion properties of the TM1 and TM2 in the N-terminal half molecule are different. The proper membrane anchorage of both TM1 and TM2 of the MDR3 Pgp is affected by their C-terminal amino acid sequences, whereas only the membrane insertion of the TM1 is dependent on the N-terminal amino acid sequences. The efficient membrane insertion of TM3 and TM5 of MDR3 Pgp, on the other hand, requires the presence of the putative TM4 and TM6, respectively. The TM8 in the C-terminal half does not contain an efficient stop-transfer activity. These observations suggest that the membrane insertion of putative TM segments in the human MDR3 Pgp does not simply follow the prevailing sequential event of the membrane insertion by signal-anchor and stop-transfer sequences. These results, together with my previous findings, suggest that different isoforms of Pgp can be used in comparison as a model system to understand the molecular mechanism of topogenesis of polytopic membrane proteins.
APA, Harvard, Vancouver, ISO, and other styles
2

Tanikawa, Yoshihiro, So MIYAHARA, and Takayuki KAKUDA. "Evaluation of Simple Screening for Attention Functions." International Journal of Medical Science and Clinical Invention 9, no. 06 (June 24, 2022): 6164–69. http://dx.doi.org/10.18535/ijmsci/v9i06.07.

Full text
Abstract:
We hypothesized that a combination of multiple tasks consisting of suppression of blinking and sustaining maximum muscle force in pinching serves as a simple screening test for declines in attention functions and investigated the relationships of suppression of blinking, the number of blinks during measurement of pinch strength, and attention functions. The subjects were 27 young individuals (10 males and 17 females with a mean age of 31.9±13.8 years) and 12 elderly individuals (2 males and 10 females with a mean age of 73.3±5.6 years). Part A and Part B of a Japanese version of the Trail Making Test (TMT) were used to evaluate attention functions. The subjects were instructed before the measurement of pinch strength to stop blinking for 5 seconds, and the number of blinks was counted. Pinch strength was measured for 5 seconds, and its changes were recorded. No instructions were given in the first measurement of pinch strength, but the second measurement was performed after the instruction of “Do not blink” (with restriction of blinking). TMT-Part A with restriction of blinking took more time in the group that blinked than in the group that did not blink (41.1±16.4 vs. 25.6±8.7, p<0.01). The subjects who required more time to execute TMT-Part A tended to be more likely to blink even under restriction. Examination of the presence or absence of blinking under restriction before the measurement of pinch strength is considered to be potentially useful as a simple screening test for attention functions.
APA, Harvard, Vancouver, ISO, and other styles
3

Harahap, Herpan Syafii, and Yanna Indrayana. "Obstructive Sleep Apnea Risk Level Affect Executive Function Rather than Attention." Jurnal Kesehatan Masyarakat 14, no. 2 (November 5, 2018): 272–78. http://dx.doi.org/10.15294/kemas.v14i2.14685.

Full text
Abstract:
Obstructive sleep apnea (OSA) is a kind of sleep disorders which is associated with cognitive impairment, either independently or by its strong association with metabolic syndrome. OSA affected attention and executive functions. Since the diagnosis of OSA had limitation, the assessment of OSA risk level by using STOP-BANG Questionnaire instrument in common population is important. This was a cross-sectional study aimed to investigate the effect of OSA risk level determined based on STOP-BANG Questionnaire score to attention and executive functions in 82 subjects. The demographic and clinical characteristics data obtained were age, gender, level of education, hypertension, body mass index (BMI), neck circumference, OSA risk level, and attention and executive functions. Attention function was assessed by using Forward Digit Span and Trailmaking Test A (TMT-A) instruments, while executive function was assessed by using Backward Digit Span and Verbal Fluency Test instruments. The demographic and clinical characteristics data showed significant higher proportion of male gender, large neck circumference, and hypertension in high risk OSA group. The OSA risk level significantly impaired the executive function but did not impaired attention function.
APA, Harvard, Vancouver, ISO, and other styles
4

Anderson, Neil, and Sein Htoon. "Stop the tit for tat." Physics World 15, no. 4 (April 2002): 20. http://dx.doi.org/10.1088/2058-7058/15/4/29.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Valentin, Clarisse, Patrice Dunoyer, Guillaume Vetter, Catherine Schalk, André Dietrich, and Salah Bouzoubaa. "Molecular Basis for Mitochondrial Localization of Viral Particles during Beet Necrotic Yellow Vein Virus Infection." Journal of Virology 79, no. 15 (August 1, 2005): 9991–10002. http://dx.doi.org/10.1128/jvi.79.15.9991-10002.2005.

Full text
Abstract:
ABSTRACT During infection, Beet necrotic yellow vein virus (BNYVV) particles localize transiently to the cytosolic surfaces of mitochondria. To understand the molecular basis and significance of this localization, we analyzed the targeting and membrane insertion properties of the viral proteins. ORF1 of BNYVV RNA-2 encodes the 21-kDa major coat protein, while ORF2 codes for a 75-kDa minor coat protein (P75) by readthrough of the ORF1 stop codon. Bioinformatic analysis highlighted a putative mitochondrial targeting sequence (MTS) as well as a major (TM1) and two minor (TM3 and TM4) transmembrane regions in the N-terminal part of the P75 readthrough domain. Deletion and gain-of-function analyses based on the localization of green fluorescent protein (GFP) fusions showed that the MTS was able to direct a reporter protein to mitochondria but that the protein was not persistently anchored to the organelles. GFP fused either to MTS and TM1 or to MTS and TM3-TM4 efficiently and specifically associated with mitochondria in vivo. The actual role of the individual domains in the interaction with the mitochondria seemed to be determined by the folding of P75. Anchoring assays to the outer membranes of isolated mitochondria, together with in vivo data, suggest that the TM3-TM4 domain is the membrane anchor in the context of full-length P75. All of the domains involved in mitochondrial targeting and anchoring were also indispensable for encapsidation, suggesting that the assembly of BNYVV particles occurs on mitochondria. Further data show that virions are subsequently released from mitochondria and accumulate in the cytosol.
APA, Harvard, Vancouver, ISO, and other styles
6

Zhang, Jian-Ting, Mingang Chen, Ernest Han, and Changsen Wang. "Dissection of De Novo Membrane Insertion Activities of Internal Transmembrane Segments of ATP-Binding-Cassette Transporters: Toward Understanding Topological Rules for Membrane Assembly of Polytopic Membrane Proteins." Molecular Biology of the Cell 9, no. 4 (April 1998): 853–63. http://dx.doi.org/10.1091/mbc.9.4.853.

Full text
Abstract:
The membrane assembly of polytopic membrane proteins is a complicated process. Using Chinese hamster P-glycoprotein (Pgp) as a model protein, we investigated this process previously and found that Pgp expresses more than one topology. One of the variations occurs at the transmembrane (TM) domain including TM3 and TM4: TM4 inserts into membranes in an Nin-Cout rather than the predicted Nout-Cin orientation, and TM3 is in cytoplasm rather than the predicted Nin-Coutorientation in the membrane. It is possible that TM4 has a strong activity to initiate the Nin-Cout membrane insertion, leaving TM3 out of the membrane. Here, we tested this hypothesis by expressing TM3 and TM4 in isolated conditions. Our results show that TM3 of Pgp does not have de novo Nin-Cout membrane insertion activity whereas TM4 initiates the Nin-Cout membrane insertion regardless of the presence of TM3. In contrast, TM3 and TM4 of another polytopic membrane protein, cystic fibrosis transmembrane conductance regulator (CFTR), have a similar level of de novo Nin-Cout membrane insertion activity and TM4 of CFTR functions only as a stop-transfer sequence in the presence of TM3. Based on these findings, we propose that 1) the membrane insertion of TM3 and TM4 of Pgp does not follow the sequential model, which predicts that TM3 initiates Nin-Cout membrane insertion whereas TM4 stops the insertion event; and 2) “leaving one TM segment out of the membrane” may be an important folding mechanism for polytopic membrane proteins, and it is regulated by the Nin-Cout membrane insertion activities of the TM segments.
APA, Harvard, Vancouver, ISO, and other styles
7

Meini, Stefania, Paola Cucchi, Sabrina Zappitelli, Luigi Rotondaro, Laura Quartara, Alessandro Giolitti, and Carlo Alberto Maggi. "Preliminary mutational analysis of the human kinin B2 receptor for nonpeptide antagonist ligands recognition." Canadian Journal of Physiology and Pharmacology 80, no. 4 (April 1, 2002): 303–9. http://dx.doi.org/10.1139/y02-027.

Full text
Abstract:
FR173657, LF16,0335, and LF16,0687 are nonpeptide antagonists, endowed with high affinity and selectivity for the human kinin B2 receptor. The kinin B2 receptor belongs to the family of G-protein-coupled receptors with seven transmembrane (TM) helices. In the present study, we aimed, through computer-assisted modeling and mutagenesis, to identify residues in the human B2 receptor (hB2R) amino acid sequence that are involved in nonpeptide antagonist binding in order to build up experimental data as a first step towards a molecular model of nonpeptide ligands binding site. Fourteen amino acid residues within the TM segments were mutated to alanine. The wild type and mutant receptors were stably expressed in Chinese hamster ovary (dhfr–) cells and tested for their ability to bind agonist ([3H]bradykinin) and peptide antagonist ([3H]MEN11270) radioligands. The affinity of nonpeptide ligands was determined by heterologous competition experiments using the above radioligands. We found that some mutations in TM2 (W86A) and TM7 (Y295A, N297A) impair the binding affinity of the three nonpeptide antagonists. On the other hand, some mutated residues in TM3 (S117A) and TM6 (W256A) reduce the affinity of LF16,0335 and LF16,0687 only. Results are discussed in order to build up a hypothesis for the likely different interactions of various nonpeptide ligands with the B2 receptor.Key words: binding, bradykinin B2 receptor, G-protein-coupled receptor, mutagenesis, nonpeptide.
APA, Harvard, Vancouver, ISO, and other styles
8

Schechter, Joel. "Charlotte Charke’s Tit for Tat; or, Comedy and Tragedy at War: A lost play recovered?" Studies in Theatre & Performance 33, no. 1 (January 1, 2013): 91–95. http://dx.doi.org/10.1386/stap.33.1.91_1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Lessard, Sabin. "Cooperation is less likely to evolve in a finite population with a highly skewed distribution of family size." Proceedings of the Royal Society B: Biological Sciences 274, no. 1620 (May 22, 2007): 1861–65. http://dx.doi.org/10.1098/rspb.2007.0366.

Full text
Abstract:
In the context of the finitely repeated Prisoner's Dilemma with the possibility of cooperating or defecting each time, the strategy tit-for-tat (TFT) consists in cooperating the first time and copying the strategy previously used by the opponent the next times. Assuming random pairwise interactions in a finite population of always defecting individuals, TFT can be favoured by selection to go to fixation following its introduction as a mutant strategy. We deduce the condition for this to be the case under weak selection in the framework of a general reproduction scheme in discrete time. In fact, we show when and why the one-third rule for the evolution of cooperation holds, and how it extends to a more general rule. The condition turns out to be more stringent when the numbers of descendants left by the individuals from one time-step to the next may substantially differ. This suggests that the evolution of cooperation is made more difficult in populations with a highly skewed distribution of family size. This is illustrated by two examples.
APA, Harvard, Vancouver, ISO, and other styles
10

CHO, WonYong. "Standards and means for national Integration post presidential election: Focus on TFT(Tit-for-Tat) strategy based on constitutionalism." Korean Constitutional Law Association 28, no. 3 (September 30, 2022): 61–96. http://dx.doi.org/10.35901/kjcl.2022.28.3.61.

Full text
Abstract:
After the presidential election, which inevitably causes conflict and division, there has always been a discussion of national unity. A consolidation committee with a similar name has been established and operated for tax purposes. However, there is no true definition of national unity. This study determined and defined the meaning of true national unity based on constitutional studies and analyzed the specific method of national unity using Axelrod's TFT strategy. After the presidential election, the unification discussion that pursues the abstract good without justice can be used as an indulgence. Until now, integration or national integration has not been properly defined in academia. The researcher defines national unity after the presidential election as “immediate punishment and forgiveness based on the rule of law against the anti-constitutional forces (which pursued private rather than national interest)”. “Immediate punishment and forgiveness based on the rule of law against the anti-constitutional forces (pursuing private rather than national interest)” consists of two major steps. The first step is immediate punishment, and the second step is inclusion. Punishment based on arbitrary interpretation would be political retaliation, but punishment through the rule of law against a political group that did not direct public or national interest is not political retaliation. As much as punishing innocent people, it is also wrong to have no punishment for a political group that does not pursue national interests. No one says mixing clean and dirty water is integration. True integration starts with turning dirty water into clean water.
APA, Harvard, Vancouver, ISO, and other styles
11

Li, Jie, Jianli Guo, Xiaomin Ou, Mingfeng Zhang, Yuezhou Li, and Zhenfeng Liu. "Mechanical coupling of the multiple structural elements of the large-conductance mechanosensitive channel during expansion." Proceedings of the National Academy of Sciences 112, no. 34 (August 10, 2015): 10726–31. http://dx.doi.org/10.1073/pnas.1503202112.

Full text
Abstract:
The prokaryotic mechanosensitive channel of large conductance (MscL) is a pressure-relief valve protecting the cell from lysing during acute osmotic downshock. When the membrane is stretched, MscL responds to the increase of membrane tension and opens a nonselective pore to about 30 Å wide, exhibiting a large unitary conductance of ∼3 nS. A fundamental step toward understanding the gating mechanism of MscL is to decipher the molecular details of the conformational changes accompanying channel opening. By applying fusion-protein strategy and controlling detergent composition, we have solved the structures of an archaeal MscL homolog from Methanosarcina acetivorans trapped in the closed and expanded intermediate states. The comparative analysis of these two new structures reveals significant conformational rearrangements in the different domains of MscL. The large changes observed in the tilt angles of the two transmembrane helices (TM1 and TM2) fit well with the helix-pivoting model derived from the earlier geometric analyses based on the previous structures. Meanwhile, the periplasmic loop region transforms from a folded structure, containing an ω-shaped loop and a short β-hairpin, to an extended and partly disordered conformation during channel expansion. Moreover, a significant rotating and sliding of the N-terminal helix (N-helix) is coupled to the tilting movements of TM1 and TM2. The dynamic relationships between the N-helix and TM1/TM2 suggest that the N-helix serves as a membrane-anchored stopper that limits the tilts of TM1 and TM2 in the gating process. These results provide direct mechanistic insights into the highly coordinated movement of the different domains of the MscL channel when it expands.
APA, Harvard, Vancouver, ISO, and other styles
12

Malekzadeh, Samira, Mohammad Amin Edalatmanesh, Davood Mehrabani, and Mehrdad Shariati. "Dental Pulp Stem Cells Transplantation Improves Passive Avoidance Memory and Neuroinflammation in Trimethyltin-Induced Alzheimer’s Disease Rat Model." Galen Medical Journal 10 (December 31, 2021): e2254. http://dx.doi.org/10.31661/gmj.v10i0.2254.

Full text
Abstract:
Background: According to the increasing incidence of Alzheimer’s disease (AD), this study aimed to investigate the effect of dental pulp stem cells (DPSCs) transplantation on passive avoidance memory and neuroinflammation in trimethyltin (TMT)-induced AD rat model. Materials and Methods: In this experimental study, 18 male Wistar rats were randomly divided into three groups: the control that rats received 8 mg/kg TMT plus 0.5 ml phosphate buffered saline (PBS) and TMT+DPSCs (TMT + 1×106 cells/ml DPSC in 0.5 ml PBS) groups. Then, after one month, passive avoidance test was performed. Also measured the Nuclear Factor Kappa-β (NF-Kβ) serum level and the percentage of damaged neurons in the hippocampus were determined. Results: DPSCs transplantation showed significantly increased step-through latency to the dark compartment in comparison with control and TMT+PBS groups in 24 hours after shock. Also, time spent in the dark compartment of TMT+DPSCs significantly decreased compared to control and TMT+PBS groups in 24 and 48 hours after shock (P<0.05). Furthermore, DPSCs transplantation significantly decreased the NF-Kβ serum level and percentage of damaged pyramidal neurons of CA1 compared with TMT+PBS (P<0.05). Conclusion: DPSCs transplantation improved memory and learning, regulated NF-Kβ serum level, and decreased damage neurons of CA1 hippocampus in TMT-induced AD rat model.
APA, Harvard, Vancouver, ISO, and other styles
13

Akbar, S., Z. Ahmad, M. S. Awan, M. Farooque, and A. Ali. "Development of Fe-Cr-Co Permanent Magnets by Single Step Thermo-Magnetic Treatment." Key Engineering Materials 510-511 (May 2012): 507–12. http://dx.doi.org/10.4028/www.scientific.net/kem.510-511.507.

Full text
Abstract:
The present work is focused on a new approach for the development of Fe-Cr-Co based permanent magnets. Fe-Cr-Co alloy was prepared by using tri arc melting technique under inert atmosphere of Argon. Solution treatment was done at a temperature of 1250°C for five hours followed by water quenching and then a single step thermo-magnetic treatment (TMT) was applied at predetermined cooling rates. The influence of TMT and cooling rates on the final magnetic properties of the alloy were investigated. The results reveal that microstructure and magnetic properties were sensitive to both cooling rates & TMT and can be optimized by controlling the processing conditions. The optimum magnetic properties in the alloy with two different cooling rates of 1°C per minute and 2°C per minute were obtained as (i) 1010 Oe (Hc), 9400 G (Br), 3.4 MGOe (BHmax) (ii) 810 Oe (Hc), 10590 G (Br), 3.6 MGOe (BHmax) respectively. The above method provides a quick and low cost manufacturing route for the Fe-Cr-Co based permanent magnets with comparable magnetic properties to that of Alnico with added advantage of having high ductility.
APA, Harvard, Vancouver, ISO, and other styles
14

Seadle, Michael. "Copyright in the networked world: orphaned copyrights." Library Hi Tech 23, no. 3 (September 1, 2005): 453–59. http://dx.doi.org/10.1108/07378830510621838.

Full text
Abstract:
PurposeThis column aims to look at the results of the US Copyright Office's request for comments about orphan copyrights.Design/methodology/approachIt uses a form of Game Theory called the Prisoner's Dilemma Game to analyze the comments that are available on the Copyright Office web site.FindingsSome change seems likely, if only because the opponents of change may discover that they can gain more for themselves when they stop defending the interests of those who have abandoned their copyrights already.Practical implicationsIf some form of cooperation between intellectual property consumers and rights holders could be worked out for orphan copyrights, it might lead to further “tit‐for‐tat” reactions that help to address other copyright issues.Originality/valueProvides useful information on orphan copyrights.
APA, Harvard, Vancouver, ISO, and other styles
15

Osuka, Yosuke, Hunkyung Kim, Yutaka Watanabe, Yu Taniguchi, Narumi Kojima, Satoshi Seino, Hisashi Kawai, et al. "A Stepping Trail Making Test as an Indicator of Cognitive Impairment in Older Adults." Journal of Clinical Medicine 9, no. 9 (September 2, 2020): 2835. http://dx.doi.org/10.3390/jcm9092835.

Full text
Abstract:
This study aimed to examine the concurrent validity of a novel motor-cognitive dual-task test, the Stepping Trail Making Test (S-TMT), as an indicator of cognitive impairment (CI), and compare its screening performance to that of motor or cognitive tests alone. This was a population-based cross-sectional study including 965 Japanese adults aged ≥ 70 years. To measure the time taken to perform the S-TMT, the participants were instructed to step on 16 numbers in sequence as quickly and accurately as possible. Motor and cognitive functions were assessed by gait speed and TMT part A (TMT-A), respectively. Participants were classified into CI (< 24 points), mild CI (MCI, 24–27 points), and intact cognition (> 27 points) categories based on their Mini-Mental State Examination score. Binary logistic regression models showed that the addition of the S-TMT to the covariates model gave the highest discrimination index (c-statistics), and significantly improved reclassification indices (net reclassification improvement and integrated discrimination improvement) for screening both CI and MCI compared to those of gait speed or TMT-A alone. These results show that S-TMT has a concurrent validity as a dual-task test for screening CI and MCI and better discrimination and reclassification performance than motor or cognitive tests alone in older adults.
APA, Harvard, Vancouver, ISO, and other styles
16

Behrens, Bernd-Arno, Kai Brunotte, Tom Petersen, and Julian Diefenbach. "Mechanical and Thermal Influences on Microstructural and Mechanical Properties during Process-Integrated Thermomechanically Controlled Forging of Tempering Steel AISI 4140." Materials 13, no. 24 (December 17, 2020): 5772. http://dx.doi.org/10.3390/ma13245772.

Full text
Abstract:
Thermomechanical treatment (TMT) describes the effect of thermal and mechanical conditions on the microstructure of materials during processing and offers possible integration in the forging process. TMT materials exhibit a fine-grained microstructure, leading to excellent mechanical properties. In this study, a two-step TMT upsetting process with intermediate cooling is used to demonstrate possibilities for a process-integrated treatment and corresponding properties. A water–air-based cooling system was designed to adjust different phase configurations by varying the target temperature and cooling rate. Four different thermal processing routes and four combinations of applied plastic strains are investigated in standardized mechanical tests and metallographic analyses. The applied TMT results in a finely structured bainitic microstructure of the investigated tempering steel AISI 4140 (42CrMo4) with different characteristics depending on the forming conditions. It can be shown that the demands of the standard (DIN EN ISO 683) in a quenched and tempered state can be fulfilled by means of appropriate forming conditions. The yield strength can be enhanced up to 1174 MPa while elongation at break is about 12.6% and absorbed impact energy reaches 58.5 J without additional heat treatment when the material is formed after rapid cooling.
APA, Harvard, Vancouver, ISO, and other styles
17

Martinova, Z., and G. Zlateva. "Microstructure development during thermomechanical treatment of Al-Mg-Si alloy." Journal of Mining and Metallurgy, Section B: Metallurgy 38, no. 3-4 (2002): 153–62. http://dx.doi.org/10.2298/jmmb0204153m.

Full text
Abstract:
The effect of natural aging and 95% cold deformation on the microstructure evolution and aging characteristics in commercial Al - 1 mass % Mg2Si alloy subjected to thermomechanical treatment (TMT) was examined. Transmission electron microscopy observations, tensile tests and electrical conductivity measurements were carried out in order to correlate microstructural features to properties on each TMT step. It was established that pre-aging at room temperature affected the morphology of dislocation structure induced by next cold deformation. The observed transition from cellular to homogenous dislocation distribution was explained by the different stability of zones produced by pre-aging of different duration. Natural aging suppressed recovery processes during post-deformation artificial aging, especially after prolonged storage after quenching and at lower aging temperature. It influenced the morphology of precipitates produced by post deformation artificial aging also. The overall effect of TMT involving prior-deformation natural aging in the scheme, on hardness, tensile properties and electrical conductivity is discussed based on experimental microstruture observations.
APA, Harvard, Vancouver, ISO, and other styles
18

Sriramatr, Sonthaya, and Raweewan Maphong. "The Acute Effects of Actively Play on the ExecutiveFunctions of Thai Children." Physical Activity Review 10, no. 1 (2022): 1–9. http://dx.doi.org/10.16926/par.2022.10.01.

Full text
Abstract:
Background: This study aimed to investigate the effects of a single active play intervention on the executive functions in children. Methods: A quasi-experimental design. Children from two classrooms in the 5th grade were randomly selected. Children in one classroom (n=30) were assigned to an active playgroup, while those from another class (n=30) were assigned to a control group. We tested two components of executive functions (i.e., working memory [the Trails Making Test (TMT)] and inhibitory control [the Stoop Color-Word Test (SCWT)]) at pre-and post-intervention times. Results: There was a significant interaction effect of an active play by time for the TMT and SCWT (p < 0.01). At post-test, children in the active playgroup had better TMT and SCWT scores than those in the control group (p < 0.05). Compared to the pre-test, children in the active playgroup had better TMT and SCWT cores on the post-test (p<0.01), while children in the control group had better TMT1 and SCWT2 scores in the post-test (all p<0.05). Conclusion: Given the improved working memory and inhibitory control, the active play seems to be an effective intervention, even in a single bout.
APA, Harvard, Vancouver, ISO, and other styles
19

Snure, Michael, Michael J. Motala, Timothy A. Prusnick, Evan M. Smith, David Moore, Christopher Muratore, Shivashankar R. Vangala, and Nicholas R. Glavin. "Two step synthesis of ultrathin transition metal tellurides." Journal of Vacuum Science & Technology A 40, no. 4 (July 2022): 042202. http://dx.doi.org/10.1116/6.0001893.

Full text
Abstract:
Transition metal tellurides (TMTs) are an exciting group of two-dimensional materials with a wide variety of polytypes and properties. Here, we demonstrate a simple and versatile two-step method for producing MoTe2, WTe2, and PtTe2 films via tellurization of thin metals at temperatures between 400 and 700 °C. Across this temperature range, monoclinic 1T′ phase of MoTe2, orthorhombic Td phase of WTe2, and hexagonal 2H phase of PtTe2 were formed. Based on x-ray diffraction and Raman analysis, temperatures greater than 600 °C were found to produce the best quality MoTe2 and WTe2. In contrast, lower temperatures (400 °C) were preferred for PtTe2, which becomes discontinuous and eventually decomposes above 650 °C. The presence of H2 in the tellurization process was critical to facilitate the formation of H2Te, which is known to be more reactive than Te vapor. In the absence of H2, neither MoTe2 nor WTe2 formed, and although PtTe2 was formed under pure N2, the crystal quality was significantly reduced. Temperature-dependent resistivity (ρ) measurements were performed on the best quality TMT films revealing all films to be highly conductive. MoTe2 showed metallic behavior up to 205 K where it underwent a phase transition from the semimetallic Td to semiconducting 1T′ phase. WTe2 exhibited a consistent semiconducting behavior with a small positive increase in ρ with decreasing temperature, and PtTe2 showed a metallic dependence from 10 K up to room temperature. Spectroscopic ellipsometry for TMT films provides complex optical constants n and k from ultraviolet to infrared.
APA, Harvard, Vancouver, ISO, and other styles
20

Wisinger, Amanda M., Phoebe Ka Yin Tse, Karen S. Basurto, Maximillian A. Obolsky, Matthew S. Phillips, Gabriel I. Ovsiew, Zachary J. Resch, Jason R. Soble, and Kyle J. Jennette. "A-227 A Story Told in Four Parts: Embedded Validity Indicators’ Effectiveness in Predicting Dot Counting Test Validity Classification." Archives of Clinical Neuropsychology 37, no. 6 (August 17, 2022): 1383. http://dx.doi.org/10.1093/arclin/acac060.227.

Full text
Abstract:
Abstract Objective: Combining multiple embedded performance validity tests (PVTs) can produce similar classification accuracy to freestanding PVTs. However, there is a lack of research on the incremental predictive power of various combinations of PVTs. Thus, we compared eight embedded PVTs to assess those that best predict classification accuracy on the Dot Counting Test (DCT). Method: Sample included 225 patients (mean age=45.96; mean education=13.96; 56% female, 44% male; 35% White, 40% Black, 17% Hispanic, 5% Asian, 2% Other) undergoing neuropsychological evaluation that included multiple embedded PVTs, including: Brief Visuospatial Memory Test-Revised Recognition Discrimination (BVMT-R RD), Stroop Color and Word Test Word Reading T-Score (Stroop-T), Trail Making Test Part A T-Score (TMT-A), Rey Auditory Verbal Learning Test (RAVLT) Forced Choice, RAVLT Effort Score, Digit Span Age Corrected Scaled Score, Reliable Digit Span, and Letter Fluency T-Score. Patients were classified into valid/invalid groups based on four independent criterion PVTs. Results: A forward stepwise logistic regression was performed to predict DCT pass/fail using the aforementioned embedded PVTs as predictors. The model was achieved in three steps (p&lt;.05); Step 1: BVMT-R RD (Classification Accuracy=87.6%; Nagelkerke R2 =.30), Step 2: BVMT-R RD + Stroop-T (Classification Accuracy=89.6%; Nagelkerke R2 =.44); Step 3: BVMT-R RD + Stroop-T + TMT-A (Classification Accuracy=90.1%; Nagelkerke R2 =.49). Conclusion: BVMT-R RD + Stroop-T + TMT-A reliably predicted the DCT pass/fail group. Thus, this combination of embedded PVTs may be reliable predictors of validity classification when time prohibits delivery of freestanding PVTs, such as the DCT.
APA, Harvard, Vancouver, ISO, and other styles
21

Van Schil, P. E., P. Baas, R. Gaafar, A. P. Maat, M. van de Pol, B. Hasan, H. M. Klomp, A. M. Abdelrahman, J. Welch, and J. Van Meerbeeck. "Phase II feasibility trial of induction chemotherapy (ICT) followed by extrapleural pneumonectomy (EPP) and postoperative radiotherapy (PORT) for cT3N1M0 or less malignant pleural mesothelioma (MPM) (EORTC 08031)." Journal of Clinical Oncology 27, no. 15_suppl (May 20, 2009): 7509. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.7509.

Full text
Abstract:
7509 Background: MPM is a highly lethal disease and the role of EPP in the treatment of early stage, potentially resectable MPM remains controversial. EORTC 08031 phase II trial investigated the feasibility of trimodality therapy (TMT) consisting of ICT followed by EPP and PORT. Methods: Eligibility criteria were cT3N1M0 or less, proven MPM, < 70 years, PS 0–1, fit for TMT. ICT consisted of 3 courses of cisplatin 75mg/m2 and pemetrexed 500mg/m2 q3weeks. Non-progressing patients (pts) underwent EPP followed by PORT (54Gy, 30 fractions). Primary endpoint was “success of treatment” defined as a patient receiving the full protocol treatment, still alive 90 days after end of treatment without progression and without grade (G) 3–4 toxicity. Using a one step Fleming design 52 patients and 26 successes were required. Secondary endpoints were toxicity of TMT, overall and progression-free survival. Results: 59 pts were registered between 07/26/05 and 08/24/07, 1 was ineligible. Median age was 57 years (range 26–67), M/F 46/12, all proven MPM (31 epithelial, 18 mixed, 9 other). All pts underwent mediastinoscopy, cT1/T2/T3: 36/16/6, cN0/N1: 57/1. 55 pts received 3 cycles of ICT. G3–4 toxicity related to ICT was rare. 46 pts (79%) were operated, 42 (74%) had EPP. R0/1/2: 30/10/3, 6 were re-operated, pT0/1/2/3/4: 2/5/19/15/4, pN0/1/2/3: 34/2/6/2, 90-day mortality: 3 pts (6.5%); in 38 pts (83%) postoperative complications occurred. PORT was initiated in 38 pts and completed in 37 (65%). After PORT 2 pts (3.5%) died due to infection. Persisting G3/4 toxicity after 90 days: 3 pts (5.3%) due to radiation pneumonitis and bronchopleural fistula. After median follow- up of 19.3 months (mos) median overall survival time was 18.4 mos (95% CI 14.8-NR) and median progression-free survival was 13.9 mos (95% CI 10.9–17.1). Only 24 pts (42%) met the definition of success (one-sided 90% CI 0.36–1.00). Conclusions: EORTC 08031 investigated the feasibility of TMT in pts with proven MPM. Due to the low proportion of “successes” this TMT is not considered to be feasible. Although overall results were similar to other series, adjustments to this TMT are necessary. No significant financial relationships to disclose.
APA, Harvard, Vancouver, ISO, and other styles
22

Wu, Yiming, Meiling Jin, Kevyn Hart, Aijun Liao, Stacey M. Fernandes, Tinisha McDonald, Lucy Brown, et al. "Integrative Transcriptome and Quantitative Proteome Analyses Identify METTL3 As a Key Regulator for Aberrant RNA Processing in Chronic Lymphocytic Leukemia." Blood 136, Supplement 1 (November 5, 2020): 12. http://dx.doi.org/10.1182/blood-2020-139841.

Full text
Abstract:
Aberrant mRNA processing is known to drive the pathogenesis of chronic lymphocytic leukemia (CLL). Recurrent gene mutations in the RNA splicing factor SF3B1 and widespread RNA intronic polyadenylation impact genome-wide gene expression and inactivate tumor suppressors, respectively. Nevertheless, how mRNA processing is regulated and exerts its function in CLL remain elusive. To comprehensively characterize the role of mRNA processing in CLL, we performed RNA sequencing (RNA-seq) and Tandem Mass Tag (TMT) proteomics using normal and CLL B cells derived from healthy donors (n=5) and untreated CLL patients (n=22). We detected 328 proteins differentially expressed between normal and CLL B cells (|Log2FC|&gt;0.58, q&lt;0.05). Gene set enrichment analysis (GSEA) revealed that proteins involved in RNA metabolism (transcription, splicing, modification, 3'end processing, nuclear export, decay) were upregulated in CLL, while those impacting translation were downregulated. These findings were validated by immunoblotting in an independent set of samples (n=10). However, we observed no significant gene expression changes of RNA metabolism at the transcript level, indicating that regulation of these proteins occurred post-transcriptionally. Since N6-methyladenosine (m6A) is the most abundant RNA internal modification and has emerged as a key regulator for RNA metabolism, we sought to determine whether m6A is dysregulated in CLL cells. With an m6A dotblot assay and HPLC-MS, we consistently detected increased level of m6A in mRNA from CLL cells compared with normal B cells. As one of the most upregulated proteins in CLL, METTL3 writes m6A and promotes translation efficiency through its writer and reader functions, respectively. When we knocked down (KD) METTL3 in CLL cell lines (HG3, MEC1) as well as in primary CLL cells, we observed significant cell death and growth disadvantage in CLL compared to control cells, highlighting METTL3 is essential for CLL survival. We next examined whether KD of METTL3 affects m6A and RNA translation using m6A dotblot and O-propargyl-puromycin run on assays. Loss of METTL3 had subtle impact on m6A levels but it significantly decreased protein translation (t test, p&lt;0.01) in all the cell lines tested (HG3, MEC1, JeKo-1, Mino). To define the target protein that METTL3 affects, we performed an integrated Ribosome profiling and RNA-seq analysis using HG3 and Mino cells with or without METTL3. At both transcriptome and translatome levels, loss of METTL3 significantly decreased genes enriched in the mTORC1 pathway, which has an essential role in translation (Metascape, hypergeometric test, q&lt;0.05). Furthermore, it also decreased the translation efficiencies of genes involved in mRNA processing, DNA synthesis, and cell cycle pathways. This observation suggests that upregulation of METTL3 in CLL cells may regulate protein translation of the RNA metabolism pathway. Since m6A at the stop codon region is critical for METTL3 regulating protein translation, we performed MAZTER sequencing to determine m6A modification sites in normal and CLL B cells derived from healthy donors (n=5) and untreated CLL patients (n=11). We identified 214 genes with significant differential m6A modification at the stop codon region (delta cleavage efficiency&gt;0.1, Wilcoxon rank-sum test, p&lt;0.1, within DRACA motif) between normal and CLL B cells. These genes were highly enriched for mRNA processing (Metascape, q=0.017), supporting our notion that METTL3 may modulate protein expression of mRNA processing genes by recognizing m6A modification via its reader function in CLL. Consistent with its role in regulating protein expression, we detected downregulation of splicing factors (SF3A1, SF3A2, SF3B1, U2AF1) in various METTL3 KD cell lines (HG3, MEC1, JeKo-1, Mino) at only protein level but not transcription level. These data link METTL3 upregulation with RNA metabolism protein enrichment in CLL. Altogether, our integrated analysis uncovered a novel regulatory axis of METTL3 in CLL biology. We demonstrated that CLL cells have an increased m6A modification and upregulation of METTL3 at the protein level, resulting in translation of RNA metabolism related genes through its reader function by the recognition of m6A modification. Our results collectively suggest METTL3 as a central regulator for mRNA processing in CLL and provide a rationale for targeting METTL3 in this disease. Disclosures Brown: Janssen, Teva: Speakers Bureau; Gilead, Loxo, Sun, Verastem: Research Funding; Abbvie, Acerta, AstraZeneca, Beigene, Invectys, Juno/Celgene, Kite, Morphosys, Novartis, Octapharma, Pharmacyclics, Sunesis, TG Therapeutics, Verastem: Consultancy. Rosen:Seattle Genetics: Consultancy; NeoGenomics: Consultancy; Aileron Therapeutics: Consultancy; Novartis: Consultancy; Pebromene: Consultancy; Celgene: Speakers Bureau; paradigm Medical Communications: Speakers Bureau; Abbvie: Speakers Bureau. Siddiqi:TG Therapeutics: Research Funding; Janssen: Speakers Bureau; Seattle Genetics: Speakers Bureau; Oncternal: Research Funding; BeiGene: Consultancy, Research Funding; Kite, a Gilead Company: Consultancy, Research Funding; Juno: Consultancy, Research Funding; Celgene: Consultancy, Research Funding; Pharmacyclics: Consultancy, Research Funding, Speakers Bureau; AstraZeneca: Consultancy, Research Funding, Speakers Bureau.
APA, Harvard, Vancouver, ISO, and other styles
23

Sjöberg, Mathilda, Robin Löving, Birgitta Lindqvist, and Henrik Garoff. "Sequential activation of the three protomers in the Moloney murine leukemia virus Env." Proceedings of the National Academy of Sciences 114, no. 10 (February 21, 2017): 2723–28. http://dx.doi.org/10.1073/pnas.1617264114.

Full text
Abstract:
Viral membrane fusion proteins of class I are trimers in which the protomeric unit is a complex of a surface subunit (SU) and a fusion active transmembrane subunit (TM). Here we have studied how the protomeric units of Moloney murine leukemia virus envelope protein (Env) are activated in relation to each other, sequentially or simultaneously. We followed the isomerization of the SU-TM disulfide and subsequent SU release from Env with biochemical methods and found that this early activation step occurred sequentially in the three protomers, generating two asymmetric oligomer intermediates according to the scheme (SU-TM)3→ (SU-TM)2TM → (SU-TM)TM2→ TM3. This was the case both when activation was triggered in vitro by depleting stabilizing Ca2+from solubilized Env and when viral Env was receptor triggered on rat XC cells. In the latter case, the activation reaction was too fast for direct observation of the intermediates, but they could be caught by alkylation of the isomerization active thiol.
APA, Harvard, Vancouver, ISO, and other styles
24

Jeun, Yu Jin, Yunyoung Nam, Seong A. Lee, and Jin-Hyuck Park. "Effects of Personalized Cognitive Training with the Machine Learning Algorithm on Neural Efficiency in Healthy Younger Adults." International Journal of Environmental Research and Public Health 19, no. 20 (October 11, 2022): 13044. http://dx.doi.org/10.3390/ijerph192013044.

Full text
Abstract:
To date, neural efficiency, an ability to economically utilize mental resources, has not been investigated after cognitive training. The purpose of this study was to provide customized cognitive training and confirm its effect on neural efficiency by investigating prefrontal cortex (PFC) activity using functional near-infrared spectroscopy (fNIRS). Before training, a prediction algorithm based on the PFC activity with logistic regression was used to predict the customized difficulty level with 86% accuracy by collecting data when subjects performed four kinds of cognitive tasks. In the next step, the intervention study was designed using one pre-posttest group. Thirteen healthy adults participated in the virtual reality (VR)-based spatial cognitive training, which was conducted four times a week for 30 min for three weeks with customized difficulty levels for each session. To measure its effect, the trail-making test (TMT) and hemodynamic responses were measured for executive function and PFC activity. During the training, VR-based spatial cognitive performance was improved, and hemodynamic values were gradually increased as the training sessions progressed. In addition, after the training, the performance on the trail-making task (TMT) demonstrated a statistically significant improvement, and there was a statistically significant decrease in the PFC activity. The improved performance on the TMT coupled with the decreased PFC activity could be regarded as training-induced neural efficiency. These results suggested that personalized cognitive training could be effective in improving executive function and neural efficiency.
APA, Harvard, Vancouver, ISO, and other styles
25

Shang, Fei, Yong Xiang, Rui Wang, Xiaolin Wang, Zhonghao Huang, Zhuo Xu, Shaoru Li, et al. "P-6: Development of Advanced Etch-Stop Structures Oxide TFT." SID Symposium Digest of Technical Papers 49, no. 1 (May 2018): 1212–14. http://dx.doi.org/10.1002/sdtp.12102.

Full text
APA, Harvard, Vancouver, ISO, and other styles
26

Chen, Yueyue, Deke Guo, Ming Xu, Guoming Tang, Tongqing Zhou, and Bangbang Ren. "PPtaxi: Non-Stop Package Delivery via Multi-Hop Ridesharing." IEEE Transactions on Mobile Computing 19, no. 11 (November 1, 2020): 2684–98. http://dx.doi.org/10.1109/tmc.2019.2928808.

Full text
APA, Harvard, Vancouver, ISO, and other styles
27

Arendash, Gary, Haitham Abulaban, Susan Steen, Ross Andel, Yanhong Wang, Yun Bai, Rob Baranowski, et al. "Transcranial Electromagnetic Treatment Stops Alzheimer’s Disease Cognitive Decline over a 2½-Year Period: A Pilot Study." Medicines 9, no. 8 (August 3, 2022): 42. http://dx.doi.org/10.3390/medicines9080042.

Full text
Abstract:
Background: There is currently no therapeutic that can stop or reverse the progressive memory impairment of Alzheimer’s disease (AD). However, we recently published that 2 months of daily, in-home transcranial electromagnetic treatment (TEMT) reversed the cognitive impairment in eight mild/moderate AD subjects. These cognitive enhancements were accompanied by predicted changes in AD markers within both the blood and cerebrospinal fluid (CSF). Methods: In view of these encouraging findings, the initial clinical study was extended twice to encompass a period of 2½ years. The present study reports on the resulting long-term safety, cognitive assessments, and AD marker evaluations from the five subjects who received long-term treatment. Results: TEMT administration was completely safe over the 2½-year period, with no deleterious side effects. In six cognitive/functional tasks (including the ADAS-cog13, Rey AVLT, MMSE, and ADL), no decline in any measure occurred over this 2½-year period. Long-term TEMT induced reductions in the CSF levels of C-reactive protein, p-tau217, Aβ1-40, and Aβ1-42 while modulating CSF oligomeric Aβ levels. In the plasma, long-term TEMT modulated/rebalanced levels of both p-tau217 and total tau. Conclusions: Although only a limited number of AD patients were involved in this study, the results suggest that TEMT can stop the cognitive decline of AD over a period of at least 2½ years and can do so with no safety issues.
APA, Harvard, Vancouver, ISO, and other styles
28

Battaglia, Piero A., Santina Zito, Antonella Macchini, and Franca Gigliani. "A Drosophila model of HIV-Tat-related pathogenicity." Journal of Cell Science 114, no. 15 (August 1, 2001): 2787–94. http://dx.doi.org/10.1242/jcs.114.15.2787.

Full text
Abstract:
To analyze the mechanism of Tat-mediated HIV pathogenicity, we produced a Drosophila melanogaster strain transgenic for HIV-tat gene and induced the expression of the protein during Drosophila development. By in vitro and in vivo experiments, we demonstrated that Tat specifically binds to tubulin via the MAP-binding domain of tubulin, and that this interaction delays the polymerization of tubulin and induces a premature stop to microtubule-dependent cytoplasmic streaming. The delay in the polymerization of microtubules, the tracks for the transport of the axes determinants, alters the positioning of the dorso-ventral axis as shown by the mislocalization of Gurken and Kinesin in oocyte of Drosophila after Tat induction. These results validate the use of Drosophila as a tool to study the molecular mechanism of viral gene products and suggest that Tat-tubulin interaction is responsible for neurodegenerative diseases associated with AIDS.
APA, Harvard, Vancouver, ISO, and other styles
29

Rahman, Md Arifur, Ardeshir Amirkhani, Farhana Parvin, Durdana Chowdhury, Mark P. Molloy, Anand Kumar Deva, Karen Vickery, and Honghua Hu. "One Step Forward with Dry Surface Biofilm (DSB) of Staphylococcus aureus: TMT-Based Quantitative Proteomic Analysis Reveals Proteomic Shifts between DSB and Hydrated Biofilm." International Journal of Molecular Sciences 23, no. 20 (October 13, 2022): 12238. http://dx.doi.org/10.3390/ijms232012238.

Full text
Abstract:
The Gram-positive bacterium Staphylococcus aureus is responsible for serious acute and chronic infections worldwide and is well-known for its biofilm formation ability. Recent findings of biofilms on dry hospital surfaces emphasise the failures in current cleaning practices and disinfection and the difficulty in removing these dry surface biofilms (DSBs). Many aspects of the formation of complex DSB biology on environmental surfaces in healthcare settings remains limited. In the present study, we aimed to determine how the protein component varied between DSBs and traditional hydrated biofilm. To do this, biofilms were grown in tryptic soy broth (TSB) on removable polycarbonate coupons in the CDC biofilm reactor over 12 days. Hydrated biofilm (50% TSB for 48 h, the media was then changed every 48 h with 20% TSB, at 37 °C with 130 rpm). DSB biofilm was produced in 5% TSB for 48 h at 35 °C followed by extended periods of dehydration (48, 66, 42 and 66 h at room temperature) interspersed with 6 h of 5% TSB at 35 °C. Then, we constructed a comprehensive reference map of 12-day DSB and 12-day hydrated biofilm associated proteins of S. aureus using a high-throughput tandem mass tag (TMT)-based mass spectrometry. Further pathway analysis of significantly differentially expressed identified proteins revealed that proteins significantly upregulated in 12-day DSB include PTS glucose transporter subunit IIBC (PtaA), UDP-N-acetylmuramate-L-alanine ligase (MurC) and UDP-N-acetylenolpyruvoylglucosamine (MurB) compared to 12-day hydrated biofilm. These three proteins are all linked with peptidoglycan biosynthesis pathway and are responsible for cell-wall formation and thicker EPS matrix deposition. Increased cell-wall formation may contribute to the persistence of DSB on dry surfaces. In contrast, proteins associated with energy metabolisms such as phosphoribosyl transferase (PyrR), glucosamine--fructose-6-phosphate aminotransferase (GlmS), galactose-6-phosphate isomerase (LacA), and argininosuccinate synthase (ArgG) were significantly upregulated whereas ribosomal and ABC transporters were significantly downregulated in the 12-day hydrated biofilm compared to DSB. However, validation by qPCR analysis showed that the levels of gene expression identified were only partially in line with our TMT-MS quantitation analysis. For the first time, a TMT-based proteomics study with DSB has shed novel insights and provided a basis for the identification and study of significant pathways vital for biofilm biology in this reference microorganism.
APA, Harvard, Vancouver, ISO, and other styles
30

Awa, Hart Okorie, Don Monday Baridam, and Barinedum Michael Nwibere. "Demographic determinants of electronic commerce (EC) adoption by SMEs." Journal of Enterprise Information Management 28, no. 3 (April 13, 2015): 326–45. http://dx.doi.org/10.1108/jeim-10-2013-0073.

Full text
Abstract:
Purpose – Research on the demographic characteristics of top management team (TMT) on e-commerce adoption has really advanced. Although some of such studies factored location factors as e-commerce adoption drivers, rare attempts have been made to unravel if the differences in the demographic composition of TMT and the rate of adoption may be explained by the differences in the firm’s geographical location. Therefore, the purpose of this paper is to bridge this knowledge gap by proposing a framework that conceives and measures geographical location as a contextual variable between e-commerce adoption and TMT composition. Design/methodology/approach – Data were generated from the opinions of owners/managers of 226 SMEs drawn purposefully from registered SMEs in five industries located in three geo-political zones of Nigeria. Two cities (a state capital and a commercial nerve centre) were studied and a four-step hierarchical regression (spanning factor-loading) was used to test the hypotheses. Findings – Evidence from the study shows that the hypothesized relationships between demographic factors and e-commerce adoption (main/direct effects) were statistically significant (supporting H1-H4). The two moderators (physical infrastructures and industrial specialization) that explained location factors were equally statistically significant in moderating the relationship between the demographic composition of TMT and e-commerce adoption. Research limitations/implications – Sampling the opinions of SMEs in some industries of three geo-political zones of Nigeria limits the power of generalization. Therefore, extended data and measures are required to replicate the study in order to build external validity and reliability, and possibly theories. Further, some errors seem unavoidable in the course of converting the data through SPSS procedure just as all the measures used appear subjective and prone to common method bias. Other demographic and location factors not captured in the study may be handled by future studies. Originality/value – The work will be of benefit to the academia and practitioners in terms of showing how location factors dictate the relationship between the demographic composition of top management and e-commerce adoption. The paper raises pointers that stimulate future research and advised policy-makers on even or near-even distribution of infrastructural facilities.
APA, Harvard, Vancouver, ISO, and other styles
31

Lodato, Patricia B., Elizabeth J. Rogers, and Paul S. Lovett. "A Variation of the Translation Attenuation Model Can Explain the Inducible Regulation of the pBC16 Tetracycline Resistance Gene in Bacillus subtilis." Journal of Bacteriology 188, no. 13 (July 1, 2006): 4749–58. http://dx.doi.org/10.1128/jb.01937-05.

Full text
Abstract:
ABSTRACT Expression of the tet resistance gene from plasmid pBC16 is induced by the antibiotic tetracycline, and induction is independent of the native promoter for the gene. The nucleotide sequence at the 5′ end of the tet mRNA (the leader region) is predicted to assume a complex secondary structure that sequesters the ribosome binding site for the tet gene. A spontaneous, constitutively expressed tet gene variant contains a mutation predicted to provide the tet gene with a nonsequestered ribosome binding site. Lastly, comparable levels of tet mRNA can be demonstrated in tetracycline-induced and uninduced cells. These results are consistent with the idea that the pBC16 tet gene is regulated by translation attenuation, a model originally proposed to explain the inducible regulation of the cat and erm genes in gram-positive bacteria. As with inducible cat and erm genes, the pBC16 tet gene is preceded by a translated leader open reading frame consisting of a consensus ribosome binding site and an ATG initiation codon, followed by 19 sense codons and a stop codon. Mutations that block translation of cat and erm leaders prevent gene expression. In contrast, we show that mutations that block translation of the tet leader result in constitutive expression. We provide evidence that translation of the tet leader peptide coding region blocks tet expression by preventing the formation of a secondary-structure complex that would, in the absence of leader translation, expose the tet ribosome binding site. Tetracycline is proposed to induce tet by blocking or slowing leader translation. The results indicate that tet regulation is a variation of the translation attenuation model.
APA, Harvard, Vancouver, ISO, and other styles
32

Nahidan, Mohamad Hossien, Mehdi Niroomand, and Behzad Mirzaeian Dehkordi. "Power Enhancement under Partial Shading Condition Using a Two-Step Optimal PV Array Reconfiguration." International Journal of Photoenergy 2021 (January 13, 2021): 1–19. http://dx.doi.org/10.1155/2021/8811149.

Full text
Abstract:
Under partial shading conditions, photovoltaic (PV) arrays are subjected to different irradiance levels caused by nonuniform shading. As a result, a mismatch between the modules, a reduction in the power generated, and the hotspot phenomenon will be observed. One method to reduce mismatch losses is to reconfigure the total-cross-tied (TCT) array in dynamic and static forms, where improved performance can be achieved through more efficient shading distribution thanks to increased dimensions. However, the increase in dimensions leads to the complexity of wiring and installation in static reconfiguration and the large number of switches and sensors required in dynamic reconfiguration. To rectify these problems, a two-step method is proposed in this paper. In the first step, the modules inside the PV array are divided into subarrays with wiring in static reconfiguration, rather than being wired as large-scale PV arrays. In the second step, an algorithm is developed for dynamic reconfiguration. The introduced algorithm searches for all possible connections and finally identifies the most optimal solution. As an advantage, this algorithm employs only the short-circuit current values of the subarray rows, which reduces the number of switches and sensors required in comparison to dynamic reconfiguration. Under 8 different partial shading patterns, simulations are conducted and results confirm that the proposed method outperforms the TCT array and statically modified TCT array in terms of power and mismatch losses. Among these, the highest power improvement is obtained with regard to the TCT array and statically modified TCT array under the fourth and eighth shading patterns, respectively.
APA, Harvard, Vancouver, ISO, and other styles
33

Lin, Dyi-Yih, and Li-Chi Yeh. "Evaluating Visual Performance for Older and Young Adults in Using TFT-LCD: Effects of Display Polarity, Line Spacing and Font Size." SOP Transactions on Psychology 2, no. 1 (January 31, 2015): 8–15. http://dx.doi.org/10.15764/stp.2015.01002.

Full text
APA, Harvard, Vancouver, ISO, and other styles
34

Delaney, Conor P. "Hemostatic Step-by-Step Procedure to Control Presacral Bleeding After Laparoscopic TME." World Journal of Surgery 33, no. 4 (February 21, 2009): 816. http://dx.doi.org/10.1007/s00268-008-9911-3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
35

Cujec, T. P., H. Cho, E. Maldonado, J. Meyer, D. Reinberg, and B. M. Peterlin. "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme." Molecular and Cellular Biology 17, no. 4 (April 1997): 1817–23. http://dx.doi.org/10.1128/mcb.17.4.1817.

Full text
Abstract:
The human immunodeficiency virus (HIV) encodes a transcriptional transactivator (Tat), which binds to an RNA hairpin called the transactivation response element (TAR) that is located downstream of the site of initiation of viral transcription. Tat stimulates the production of full-length viral transcripts by RNA polymerase II (pol II). In this study, we demonstrate that Tat coimmunoprecipitates with the pol II holoenzyme in cells and that it binds to the purified holoenzyme in vitro. Furthermore, Tat affinity chromatography purifies a holoenzyme from HeLa nuclear extracts which, upon addition of TBP and TFIIB, supports Tat transactivation in vitro, indicating that it contains all the cellular proteins required for the function of Tat. By demonstrating that Tat interacts with the holoenzyme in the absence of TAR, our data suggest a single-step assembly of Tat and the transcription complex on the long terminal repeat of HIV.
APA, Harvard, Vancouver, ISO, and other styles
36

Miwa, Y., J. Tanaka, A. Ueno, and K. Goto. "Development of EBV-based One-Step Tet vector." Japanese Journal of Pharmacology 82 (2000): 202. http://dx.doi.org/10.1016/s0021-5198(19)48271-1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
37

Maes, Evelyne, Dirk Valkenborg, Geert Baggerman, Hanny Willems, Bart Landuyt, Liliane Schoofs, and Inge Mertens. "Determination of Variation Parameters as a Crucial Step in Designing TMT-Based Clinical Proteomics Experiments." PLOS ONE 10, no. 3 (March 16, 2015): e0120115. http://dx.doi.org/10.1371/journal.pone.0120115.

Full text
APA, Harvard, Vancouver, ISO, and other styles
38

Roundy, Philip T., Ye Dai, Mark A. Bayer, and Gukdo Byun. "Motivated to change? TMT regulatory focus and strategic change." Management Research Review 39, no. 7 (July 18, 2016): 803–29. http://dx.doi.org/10.1108/mrr-10-2014-0235.

Full text
Abstract:
Purpose This paper aims to introduce the concept of top management team (TMT) regulatory focus to explain the differences in executive motivation. Upper echelons research has demonstrated that top managers’ willingness to deviate from their current strategies is a key determinant of organizational success. However, it is not clear why some TMTs are motivated to embrace strategic change while others are motivated to favor the strategic status quo. Design/methodology/approach Recent work in the psychology of motivation is used to develop a conceptual model explaining how the regulatory focus of TMTs can impact their outlooks toward strategic change. Findings It is theorized that there is a positive (negative) relationship between promotion (prevention)-focused TMTs and strategic change. It is also theorized that executives’ performance aspirations, firm maturity and the stability of the environment will influence the relationship between regulatory focus and strategic change. Originality/value Although the theoretical explanations provided by past research on top manager motivation are psychological in their general focus, with few exceptions research has not sought to understand the specific deep-level, socio-cognitive characteristics that shape executives’ perceptions of strategic change. By developing an understanding of the psychological determinants of strategic change, as well as the interplay between these determinants and firm- and environment-level factors, this paper represents a step in the direction of explaining why some TMTs are motivated to embrace strategic change while others seem “locked-in” to the status quo.
APA, Harvard, Vancouver, ISO, and other styles
39

Arnenda, Gussasta Levi, Bram Setyadji, and Zulkarnaen Fahmi. "Laju Tangkap, Sebaran Daerah Penangkapan dan Potensi Stok Sumber Daya Ikan Tuna Cakalang Tongkol (TCT) di Sumatera Utara." Jurnal Kelautan dan Perikanan Terapan (JKPT) 4, no. 1 (June 30, 2021): 47. http://dx.doi.org/10.15578/jkpt.v4i1.9740.

Full text
Abstract:
Potensi sumber daya perikanan tuna cakalang tongkol (TCT) di Wilayah Pengelolaan Perikanan (WPP-NRI) 572 sanagt tinggi. Salah satu wilayah perikanan di Indonesia penghasil utama komoditas ini adalah Sumatera Utara. Tujuan penelitian ini adalah untuk menganalisis laju tangkap, daerah sebaran hasil tangkapan TCT peralat tangkap di Sumatera Utara, serta kontribusinya terhadap WPP 572 dan Nasional. Penelitian ini dilaksanakan dari Januari hingga Desember 2019. Pengambilan data dilakukan secara langsung dilokasi penelitian dengan metode stratifield random sampling. Data daerah penangkapan diperoleh dari logbook penangkapan ikan yang berasal dari PSDKP PPN Sibolga dan data dari Dinas Perikanan Provinsi Sumatera Utara. Daerah penangkapan dibuat menggunakan aplikasi Q-GIS. Nilai CPUE tertinggi pada pukat cincin sebesar 10,45 ton/trip, dan terendah jaring insang sebesar 0,001 ton/trip. Nilai laju tangkap tertinggi bulan oktober sebesar 20,63 ton/trip dan terendah Agustus sebesar 9,31 ton/trip. Pendaratan hasil tangkapan lebih banyak di tangkahan. Sebaran daerah penangkapan pukat cincin dari 6º LU - 6º LS dan 85º BT - 101º BT, pancing ulur dari 4º LU - 2º LS dan 92º BT - 99º BT, dan bagan dari 3º LU - 2º LS dan 98º BT - 99º BT. Kontribusi TCT di Sumatera utara tertinggi cakalang, terendah Tongkol Komo. Kontribusi TCT Terhadap WPP 572 besar akan tetapi terhadap nasional sedikit. Hasil tangkapan TCT di lebih banyak tertangkap di ZEE, dan Teritorial dari pada di laut lepas.
APA, Harvard, Vancouver, ISO, and other styles
40

Trillingsgaard, Kasper Floe, Wei Yang, Giuseppe Durisi, and Petar Popovski. "Common-Message Broadcast Channels With Feedback in the Nonasymptotic Regime: Stop Feedback." IEEE Transactions on Information Theory 64, no. 12 (December 2018): 7686–718. http://dx.doi.org/10.1109/tit.2018.2868953.

Full text
APA, Harvard, Vancouver, ISO, and other styles
41

Higuchi, Takamasa, Sae Fujii, Hirozumi Yamaguchi, and Teruo Higashino. "Mobile Node Localization Focusing on Stop-and-Go Behavior of Indoor Pedestrians." IEEE Transactions on Mobile Computing 13, no. 7 (July 2014): 1564–78. http://dx.doi.org/10.1109/tmc.2013.139.

Full text
APA, Harvard, Vancouver, ISO, and other styles
42

Chuang, Yi-Ta, Chih-Wei Yi, Yu-Chee Tseng, Chia-Sheng Nian, and Chia-Hao Ching. "Discovering Phase Timing Information of Traffic Light Systems by Stop-Go Shockwaves." IEEE Transactions on Mobile Computing 14, no. 1 (January 1, 2015): 58–71. http://dx.doi.org/10.1109/tmc.2014.2318692.

Full text
APA, Harvard, Vancouver, ISO, and other styles
43

Liu, Changjun, Feifei Tan, Hexin Zhang, and Qijuan He. "A Novel Single-Diode Microwave Rectifier With a Series Band-Stop Structure." IEEE Transactions on Microwave Theory and Techniques 65, no. 2 (February 2017): 600–606. http://dx.doi.org/10.1109/tmtt.2016.2626286.

Full text
APA, Harvard, Vancouver, ISO, and other styles
44

Guo, Zhenzhao, and Jinbiao Xiao. "Ultracompact TE0- and TE1-pass/TM0- and TM1-stop dual-mode polarizer for 155/2 µm dual-wavelength using silicon-based cross-like hybrid plasmonic waveguides." Journal of the Optical Society of America B 37, no. 12 (November 6, 2020): 3604. http://dx.doi.org/10.1364/josab.404235.

Full text
APA, Harvard, Vancouver, ISO, and other styles
45

Hawari, Feras I., Rasha K. Bader, Nour A. Obeidat, Hiba S. Ayub, Iyad M. Ghonimat, J. Taylor Hays, and Richard D. Hurt. "Influencing Readiness and Intentions to Offer Tobacco Dependence Treatment Through Training: King Hussein Cancer Center and Global Bridges Promoting an Underutilised Specialty in the Eastern Mediterranean Region." Journal of Smoking Cessation 12, no. 3 (July 29, 2016): 173–81. http://dx.doi.org/10.1017/jsc.2016.17.

Full text
Abstract:
Introduction: Although tobacco dependence treatment (TDT) is key to tobacco control, there continues to be a shortage of services in the Eastern Mediterranean Region (EMR). Barriers to offering services include the lack of training and education.Aims: To create a network of healthcare providers (HCPs) capable of advancing TDT, King Hussein Cancer Center – the regional host for Global Bridges in the EMR – developed, implemented, and evaluated a regional TDT training programme.Methods: The programme employed a predisposing–enabling–reinforcing framework, and utilised a combination of learning techniques. The evidence-informed curriculum aimed to influence knowledge, confidence, and competence, and the evaluation mechanism was aligned with these aims.Results and discussion: Training produced a 37% and 23% gain in knowledge and TDT-specific competencies, respectively. The majority of participants reported that the workshops enhanced confidence, and 72% reported intentions for positive change. Participants rated the workshops highly, and anticipated value from joining Global Bridges network. Immediate outcomes indicate success in advancing participants’ self-efficacy and readiness to treat. Intentions for positive change may potentially be the first step in modifying practice.Conclusion: Capacity building in TDT could be the first step in meeting the need for trained HCPs through positively influencing knowledge, confidence, competence, and intentions.
APA, Harvard, Vancouver, ISO, and other styles
46

Li, Xiaoling, Yuyang Wu, Jingjing Niu, Dagang Jiang, Dan Xiao, and Cuisong Zhou. "One-step sensitive thrombin detection based on a nanofibrous sensing platform." Journal of Materials Chemistry B 7, no. 34 (2019): 5161–69. http://dx.doi.org/10.1039/c9tb01098j.

Full text
Abstract:
By integration of a new PiDSD process, CHA amplification and ThT binding, one-step fluorescent detection of thrombin can be achieved by immersing a porous and 3-direction structural B-H2-functionalized nanofibrous membrane in a testing solution.
APA, Harvard, Vancouver, ISO, and other styles
47

Trubiani, O., S. Guarnieri, R. Paganelli, and R. di Primio. "Involvement of Caspase-3 in the Cleavage of Terminal Transferase." International Journal of Immunopathology and Pharmacology 15, no. 3 (September 2002): 201–8. http://dx.doi.org/10.1177/039463200201500306.

Full text
Abstract:
To investigate the in vivo role of caspase-3 in Terminal Transferase metabolism DMSO-treated RPMI-8402, a human pre-T cell line was used. In DMSO treated samples 3H-dGTP incorporation and TdT phosphorylation occurs after 4 hours of treatment. After 8 hours cells undergo TdT proteolysis in addition to its inactivation. The cleavage of TdT into 32- and 58-KDa proteolytic fragments occurred simultaneously with the activation of Caspase-3, but preceded changes associated with the apoptotic process described after 48 hours of treatment. The Caspase-3 peptide inhibitor V, used as a specific inhibitor, prevented TdT proteolysis prolonging its activity and rescued cells from apoptosis. Our experiments suggest that TdT is a nuclear substrate for Caspase-3, the main apoptotic effector protease in many cell types, and that the cleavage of TdT represents a primary step in a signal cascade leading to pre-T cell apoptosis.
APA, Harvard, Vancouver, ISO, and other styles
48

Xiao, H., J. T. Lis, and K. T. Jeang. "Promoter activity of Tat at steps subsequent to TATA-binding protein recruitment." Molecular and Cellular Biology 17, no. 12 (December 1997): 6898–905. http://dx.doi.org/10.1128/mcb.17.12.6898.

Full text
Abstract:
Artificial recruitment of TATA-binding protein (TBP) to many eukaryotic promoters bypasses DNA-bound activator function. The human immunodeficiency virus type 1 (HIV-1) Tat is an unconventional activator that up-regulates transcription from the HIV-1 long terminal repeat (LTR) through binding to a nascent RNA sequence, TAR. Because this LTR and its cognate activator have atypical features compared to a standard RNA polymerase II (RNAP II) transcriptional unit, the precise limiting steps for HIV-1 transcription and how Tat resolves these limitations remain incompletely understood. We thus constructed human TBP fused to the DNA-binding domain of GAL4 to determine whether recruitment of TBP is one rate-limiting step in HIV-1 LTR transcription and whether Tat functions to recruit TBP. As a control, we compared the activity of the adenovirus E1b promoter. Our findings indicate that TBP tethering to the E1b promoter fully effected transcription to the same degree achievable with the potent GAL4-VP16 activator. By contrast, TBP recruitment to the HIV-1 LTR, although necessary for conferring Tat responsiveness, did not bypass a physical need for Tat in achieving activated transcription. These results document that the HIV-1 and the E1b promoters are transcriptionally limited at different steps; the major rate-limiting step for E1b is recruitment of TBP, while activation of the HIV-1 LTR requires steps in addition to TBP recruitment. We suggest that Tat acts to accelerate rate-limiting steps after TBP recruitment.
APA, Harvard, Vancouver, ISO, and other styles
49

Tabuenca, Bernardo, Marco Kalz, Stefaan Ternier, and Marcus Specht. "Stop and Think: Exploring Mobile Notifications to Foster Reflective Practice on Meta-Learning." IEEE Transactions on Learning Technologies 8, no. 1 (January 1, 2015): 124–35. http://dx.doi.org/10.1109/tlt.2014.2383611.

Full text
APA, Harvard, Vancouver, ISO, and other styles
50

Peeters, I. R., S. A. C. Wanten, L. M. Verhoef, A. Den Broeder, N. Van Herwaarden, and M. Flendrie. "POS1477-HPR GOUT PATIENTS IN REMISSION, AND THEIR PERSPECTIVES ON URATE LOWERING THERAPY TREATMENT STOP OR CONTINUATION STRATEGIES." Annals of the Rheumatic Diseases 80, Suppl 1 (May 19, 2021): 1023.2–1024. http://dx.doi.org/10.1136/annrheumdis-2021-eular.890.

Full text
Abstract:
Background:Urate lowering therapies (ULT) are used to reduce hyperuricemia in gout patients (1). When gout remission is reached, patients often ask if ULT should be continued lifelong (treat to target strategy, T2T), or if tapering or stopping (a treat to symptom approach, T2S) can be attempted. In fact, although current rheumatology guidelines (1,2) suggest continuation, conclusive evidence for this is absent. Since ULT therapy adherence also remains suboptimal, exploring gout patients’ beliefs on different long term ULT treatment strategies is of great value.Objectives:To identify cognitions and emotions on ULT treatment strategies (T2T continuation and T2S cessation) of gout patients in remission with current or previous ULT use.Methods:Purposive sampling (3) was used to recruit patients from a general practice and a rheumatology department (Nijmegen, the Netherlands), with a clinical diagnosis of gout, current or previous ULT use and remission according to adapted (without serum urate criterion) preliminary gout remission criteria(4). Semi-structured interviews were conducted by two interviewers and audio-records were fully transcribed. Inductive thematic analysis (5) was used to analyse and interpret our data using the ATLAS.ti. software.Results:From a total of 18 patients (16 male/2 female), 14 patients were treated by a rheumatologist (10 currently using ULT, 1 intermittent and 3 previously) and 4 were treated by a general practitioner (all currently using ULT). Patients were satisfied with a T2T strategy, due to the absence of flares, a feeling of certainty and the reassurance of serum urate monitoring. Reluctance towards medication was reported, the importance of indefinite ULT use was questioned and its chronic use was addressed as a drawback. Reducing medication use by a T2S strategy was assessed positively and this strategy was considered less burdensome. A wish for and the willingness to follow a T2S approach was expressed. Fear and concerns of flaring after ULT cessation were expressed and were deemed both acceptable and unacceptable. See Table 1 for a schematic overview of the results.Table 1.Overview of patients’ perspectives on ULT treatment strategiesMotivation for a T2T strategyDrawbacks of a T2T strategy1. Being free of flares2. Acceptance of and contentment with chronic ULT use3. Feels secure due to regular SU monitoring4. No desire for change1. Resistance to (any) medication use2. Side effects of ULT3. Possibly detrimental to healthcare costsMotivation for a T2S strategyDrawbacks of a T2S strategy1. Doubt if chronic ULT use is necessary a. Possible restorative capacity of the body b. Curious to effects of ULT cessation2. Being free of ULT side effects3. Long term damage ULT unknown4. Less burdensome for patient and body5. Wish for minimization of (any) medication use1. Fear and insecurities on a. Flaring and not being able to function b. Joint damage2. Feels uncontrolled3. Hassle with visits, blood tests and medication adjustments when a flare occurs.Conclusion:This study provides an overview of perspectives on ULT treatment strategies of gout patients in remission. These results must be considered in developing educational material for patients and in future research on gout management, particularly in designing randomised clinical trials on this subject.References:[1]Richette P et al. Ann Rheum Dis. 2017;76(1):29-42.[2]FitzGerald JD et al. Arthritis Care Res (Hoboken). 2020;72(6):744-60.[3]Pope C et al. Qual Saf Health Care. 2002;11(2):148-52.[4]de Lautour H et al. Arthritis Care Res (Hoboken). 2016;68(5):667-72.[5]Pope C et al. Bmj. 2000;320(7227):114-6.Acknowledgements:We would like to thank dr. Erik Bischoff for his cooperation and help in including primary care patients from his general practice UGC Heyendael, Nijmegen the Netherlands.Disclosure of Interests:None declared
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography