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1
Lund-Pero, Margaretha. "Nonspecific esterases in human tissues evidence for their involvement in steroid metabolism and in carcinogenesis /." Lund : Dept. of Molecular Ecogenetics, the Wallenberg Laboratory, University of Lund, 1995. http://catalog.hathitrust.org/api/volumes/oclc/39781861.html.
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Seymour, Beverley Lesley. "The effect of steroid hormones on the size of myometrial cells : a morphometric study." Thesis, Cape Technikon, 1997. http://hdl.handle.net/20.500.11838/1503.
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Thesis (MTech (Biomedical Technology))--Cape Technikon, Cape Town,1997The aims of this study were to measure: 1. Myometrial cells of menopausal uteri to establish whether they atrophy after the menopause. 2. Myometrial cells at different phases of the menstrual cycle to investigate the influences of oestrogen and progesterone during the cycle. 3. Myometrial cells in the fundus and lower uterine segment to establish whether they differ in size. 4. Myometrial cells of pregnant uteri to investigate the effect of the hormonal status of pregnant women on the size of myometrial cells. 5. Neoplastic cells of leiomyomas of the uterus to investigate whether these benign tumours behave in the same manner as myometrium or, because they are neoplastic, they react differently. A preliminary investigation was undertaken to establish the optimal methodology for this study to measure myometrial and leiomyoma nuclei in the uterus. The aims of this preliminary investigation were: 1. To test the reproducibility of measurements of myometrial and leiomyoma nuclei in transverse and cross section. 2. To test five histological staining methods to ascertain the best method for a morphometric study on uterine cells. 3. To find the minimum sample size of nuclei per section of myometrium or leiomyoma in order to yield statistically significant results. This preliminary study found that the Haematoxylin and Eosin stain gave the most statistically reproducible measurements. Subjective assessment of the five staining methods also found Haematoxylin and Eosin to be optimal. It was also found during the preliminary study that measuring the myometrial nuclei in cross rather than transverse section gave the most statistically reproducible measurements. It was also found that it was best to use an axial ratio criterion of 0,9 when measuring cross-sectioned myometrial nuclei. The optimum sample size per section was also investigated and it was found that measuring 100 nuclei was optimal. It was found that in the uteri used in this study there was no statistically significant decrease in nuclear size after the menopause. It was also found that there was no statistically significant difference in nuclear size during the different phases of the menstrual cycle. There was also no notable difference in nuclear size between nuclei in the fundus and lower segment of the uteri in this study. It was found that there was a significant increase in the size of nuclei in leiomyomas compared to the normal myometrial nuclei from the same patient. The myometrial nuclei from pregnant uteri were also significantly larger than those from non-gravid uteri.
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Al-Mana, D. "Ovarian steroid hormones and auditory function." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1386639/.
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Considerable anecdotal evidence and information from previous studies suggest that auditory function may be influenced by hormones. This thesis reviews in detail the potential role of hormones in modulating the auditory system and in the development of pathological conditions in the auditory system with an emphasis on the effect of the ovarian hormones. Ovarian steroids may influence auditory function directly through their receptors, which have been detected in the auditory system, or indirectly through their effects on the blood supply, the fluid electrolyte balance of the cochlea, and the neurotransmitters of the auditory system. Effects on other parts of the central nervous system connected to the auditory system may also be of importance. The aim of the study was to investigate whether physiological alterations in ovarian hormones in women with normal hearing, during the natural ovarian cycle and assisted conception treatment were associated with changes in auditory function at the cochlear and brain stem level, and whether these variations were not seen in men over a similar period of time. The auditory tests evaluated auditory function from the outer ear to the brainstem in both the afferent and efferent system. Hormone levels were assayed only in the female subjects at the same time as the auditory testing, four times during the ovarian cycle, or three times during the assisted conception treatment. Auditory tests were undertaken in the male subjects once a week for four consecutive weeks to correspond with the ovarian cycle measurements. A number of changes in auditory function were observed during the ovarian cycle and assisted conception treatment, and gender differences were noted. The OAE results may suggest either excitation of the cochlea with higher levels of oestrogen, or suppression of the cochlea with higher level of progesterone. The longer ABR latency following ovarian stimulation and in the follicular phase of the ovarian cycle is consistent with the inhibitory effect of neurosteroids on ABR associated with higher levels of oestrogen. The variation in auditory function were not observed in men.
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Farquharson, Roy G. "Fetal abstraction of placental steroid hormones." Thesis, University of Aberdeen, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328653.
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Gingnell, Malin. "Ovarian Steroid Hormones, Emotion Processing and Mood." Doctoral thesis, Uppsala universitet, Obstetrik & gynekologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-199791.
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It is known that some psychiatric disorders may deteriorate in relation to the menstrual cycle. However, in some conditions, such as premenstrual dysphoric disorder (PMDD), symptomatology is triggered mainly by the variations in ovarian steroid hormones. Although symptoms induced by fluctuations in ovarian steroids often are affective, little is known about how emotion processing in women is influenced by variations, or actual levels, of ovarian steroid hormones. The general aim of this thesis was to evaluate menstrual cycle effects on reactivity in emotion generating and controlling areas in the corticolimbic system to emotional stimulation and anticipation, in healthy controls and women with PMDD. A second aim was to evaluate corticolimbic reactivity during long-term administration of exogenous ovarian steroids. In study I, III and IV effects of the menstrual cycle on emotional reactivity in women with PMDD was studied. In study I, women with PMDD in displayed higher amygdala reactivity than healthy controls to emotional faces, not in the luteal phase as was hypothesised, but in the follicular phase. No difference between menstrual cycle phases was obtained in women with PMDD, while healthy controls had an increased reactivity in the luteal phase. The results of study I was further elaborated in study III, where women with PMDD were observed to have an increased anticipatory reactivity to negative emotional stimuli. However, no differences in amygdala reactivity to emotional stimuli were obtained across the menstrual cycle. Finally, in study IV the hypothesis that amygdala reactivity increase in the luteal phase in women with PMDD is linked to social stimuli rather than generally arousing stimuli was suggested, tested and supported. In study II, re-exposure to COC induced mood symptoms de novo in women with a previous history of COC-induced adverse mood. Women treated with COC reported increased levels of mood symptoms both as compared to before treatment, and as compared to the placebo group. There was a relatively strong correlation between depressive scores before and during treatment. The effects of repeated COC administration on subjective measures and brain function were however dissociated with increased aversive experiences accompanied by reduced reactivity in the insular cortex.
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Murai, Takahiro. "Study on the diglucuronidation reaction of steroid hormones." Kyoto University, 2008. http://hdl.handle.net/2433/136704.
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Charlton, Michael Hugh. "Theoretical studies of steroid hormones and related compounds." Thesis, University of St Andrews, 1992. http://hdl.handle.net/10023/14449.
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A theoretical study of steroidal inhibitors of the enzymes Glucose-6-Phosphate Dehydrogenase and Aromatase is presented. Both enzyme systems are of interest in the study of cancer, the latter being the final step in the biosynthesis of oestrogens which are involved in certain types of breast cancer. Two levels of theory are employed in the study, namely, Ab Initio and Semi Empirical methods. Structures and charges have been calculated using the MOPAC and GAUSSIAN programs and these have been used to model the efficacy of various inhibitors. The major tool in comparing these steroids has been the molecular electrostatic potential (MEP). Maps of the MEP and an analysis of the similarity between the MEP s of different molecules have led both to a method of assessing the activities of steroids as enzyme inhibitors and requirements for the electronic structure of the steroid binding sites within these enzymes. A molecular graphics display program has been developed to facilitate this work. It has been designed to make full use of the facilities available. The quality of the resulting display has improved greatly on what was previously available and has been of value in studies of large molecular systems. The program is written in VAX FORTRAN and uses the Graphics Kernel System (GKS) to produce graphical output and should be reasonably easy to transfer to other systems. Finally, to determine whether PM3 really is a significant advance on AM1, a comparison of the two semi empirical methods is presented. The calculated properties of steroid hormones are compared to those of both Ab Initio calculations and experimental determinations, allowing the quality of the semi empirical predictions to be assessed.
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Mercurio, S. "ROLE OF STEROID HORMONES IN ECHINOID REPRODUCTIVE BIOLOGY." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/230752.
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Echinoid reproductive cycle has been extensively studied in several species but the mechanisms regulating gametogenesis processes are still scarcely understood. Apart from environmental factors, different research have suggested a steroid role in gonad maturation and growth. Particularly, in echinoderms steroid involvement in reproduction has been suggested by both studies on seasonal changes of steroid levels during the gonadal cycle and experiments of hormone administration. Nevertheless, the steroid function in echinoid reproductive processes has not been clearly identified, probably due to the low number of studies and the big variability of results reported. Thus, the main aim of this research project was to shed light on echinoid endocrinology and, in particular, to clarify the involvement of sex-steroid hormones in sea urchin reproductive biology. This was achieved employing both in vivo and in vitro approaches.
First of all, considering the lack of studies on the development of effective cell cultures from echinoderm gonads, primary cell cultures from ovaries of the edible sea urchin Paracentrotus lividus were developed. Ovary cell phenotypes, present in culture, were identified and characterized by different microscopic techniques. Although cell cultures could be produced from ovaries at all stages of maturation, the cells appeared healthier and viable, displaying a higher survival rate, when ovaries at early stages of gametogenesis were used. In terms of culture medium, ovarian cells were successfully cultured in modified Leibovitz-15 medium, whereas poor results were obtained in Minimum Essential Medium Eagle and Medium 199. Different substrates were tested but ovarian cells completely adhered only on poly-L-lysine. To improve in vitro conditions and stimulate cell proliferation different serum-supplements were tested. Fetal Calf Serum and an originally developed Pluteus Extract resulted to be detrimental to cell survival, apparently accelerating processes of cell death. In contrast, cells cultured with sea urchin Egg Extract appeared larger and healthier, displaying an increased longevity that allowed to maintain them for up to 1 month. Overall this study provides new experimental bases and procedures for producing successfully long-term primary cell cultures from sea urchin ovaries, providing a simple and versatile experimental tool for research in echinoderm reproductive biology.
Subsequently, in vivo and in vitro experiments, specifically addressed to determine possible 17β-estradiol (E2) and testosterone (T) involvement in echinoid reproduction, were performed. An in vivo long-term experiment of steroid dietary administration was performed in adult specimens of P. lividus. The experimental plan was specifically designed in order to reduce individual variability and synchronize the experimental animals at the same starting maturative condition. We analysed and compared different reproductive parameters (Gonad Index, Maturative Index and maturative stages distribution) in 4 experimental groups: control group (CTL), E2 and T groups fed with pellets containing respectively 17β-estradiol and testosterone, and E2-4 weeks group fed with control pellets for the first 4 weeks and then treated with 17β-estradiol. This latter was chosen in order to verify the existence of a specific E2-sensitive gametogenic stage, as proposed in different asteroid species.
Possible steroid effects on P. lividus female reproduction was also investigated with an in vitro approach. Cells, isolated by ovaries in the same maturative conditions considered in the in vivo experiments, were cultured in presence of E2 and T physiological concentrations for 2 weeks. Effects on ovarian cell morphology and behaviour were investigated. In addition, steroid regulation of the Major Yolk Protein (MYP) expression was analyzed 24 and 48 hours after E2 and T exposure. According to our results, E2 and T do not markedly influence echinoid gonad maturation and, particularly, they do not promote gamete maturation. Hormonal dietary administration did not induce striking variations in the considered reproductive parameters and no effect was observed also when males and females were analyzed separately. In addition, no specific maturative stage sensitive to E2 was found, suggesting the existence of different hormonal mechanisms in asteroids and echinoids. Similar considerations could be reported taking into account the in vitro experiments. E2 and T exposure did not affect ovarian cell size and behaviour nor MYP expression.
The obtained results suggest that these hormones are not directly involved in either gamete maturation, as demonstrated for vertebrates, or in vitellogenesis processes, as reported for several asteroid species. However a possible involvement of steroids in echinoid physiology cannot be completely excluded and their role in the regulation of lipid metabolism and protein synthesis during the different reproductive stages should be strongly considered as suggested by several authors.
Further specific research on steroid hormone mode of action, physiological function and metabolism are therefore needed to completely understand echinoid reproduction and endocrinology.
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Nawaz, Zafar. "Molecular Mechanism of Action of Steroid Hormone Receptors." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc798398/.
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A novel bacterial expression system that is capable of producing high levels of soluble, stable, biologically active human vitamin D3 and estrogen receptors has been developed. The method utilizes ubiquitin fusion technology and a low temperature nalidixic acid induction of the lambda PL promoter. This system can produce large quantities of receptor antigen, but only a small fraction displays wild-type DNA and hormone binding properties. Therefore, the use of this system to overproduce receptors for crystallization studies is not practical. To overcome these problems, a 2 um based ubiquitin fusion system which allows regulated expression of the estrogen receptor in yeast (Saccharomyces cerevisiae) was developed. This system produces the estrogen receptor to a level of 0.2% of the total soluble protein. Moreover, this protein is undegradable, soluble, and biologically active. To test the transcriptional activity of the estrogen receptor produced in yeast, a cis-trans transcription assay was developed. This assay revealed that the transcriptional activity of the human estrogen receptor expressed in yeast was similar to that observed in transfected mammalian cells. This reconstituted estrogen transcription unit in Saccharomyces cerevisiae was utilized to examine the regulation of estrogen receptor functions by antiestrogens, to develop a random and rapid approach for identifying novel estrogen response elements, to characterize estrogen receptor variants cloned from human breast tumors, and to examine the effect of estrogen receptor on the regulation of osteocalcin gene.
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Fischer, Katharina. "The mineralocorticoid receptor amino terminal transactivation domain investigation of structural plasticity and protein-protein interactions /." Thesis, Available from the University of Aberdeen Library & Historic Collections Digital Resources, 2008. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24694.
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Thesis (Ph.D.)--Aberdeen University, 2008.Title from web page (viewed on Feb. 23, 2009). With: Natural disordered sequences in the amino terminal domain of nuclear receptors : lessons from the androgen and glucocorticoid receptors / Iain J. McEwan ... et al. Nuclear Receptor Signalling. 2007: 5. Includes bibliographical references.
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Ildgruben, Anna. "Human vaginal epithelial immunity and influences of hormonal contraceptive usage." Doctoral thesis, Umeå : Klinisk mikrobiologi, enh. för Immunologi och Klin. vetenskap, obstetrik och gynekologi, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-595.
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Roberts, April M. "Steroid hormone treatments alter growth characteristics in transformed human ovarian cell lines." Virtual Press, 2003. http://liblink.bsu.edu/uhtbin/catkey/1265095.
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Fahrbach, Michael. "Anaerobic degradation of steroid hormones by novel denitrifying bacteria." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=982986769.
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Jones, David G. L. "Regulation of chick fibronectin mRNA levels by steroid hormones." Thesis, University of Manchester, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.277354.
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Taiwo, Benjamin Gbenro. "Modulation of human sperm by follicular fluid steroid hormones." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7292/.
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Detailed steroid hormone profiling of human follicular fluid has paved the way for research into the modulation of human sperm by physiological concentrations of follicular fluid steroid hormones. Synthetic human follicular fluid (shFF), a novel steroid hormone analogue of human follicular fluid, based upon local data, was prepared consisting of 14 different steroid hormones including progesterone. Exposure of human spermatozoa ( > 2000 cells) to shFF stimulus at physiological and standard laboratory temperatures resulted in a rapid biphasic elevation in [Ca2+]i characterised by an initial transient Ca2+ influx immediately followed by a sustained elevation of [Ca2+]i for the duration of shFF exposure. A significant increase in the percentage of acrosome-reacted spermatozoa was observed in shFF-treated sperm (P < 0.05) however, this was significantly lower than the % AR in spermatozoa treated with progesterone alone (P < 0.01). With regards to shFF-induced sperm kinesis, a significant reduction in selected sperm motility parameters was observed 5 minutes post-incubation with shFF (P < 0.05). The study of shFF-induced chemotaxis revealed a chemokinetic effect characterised by a significant inhibition of sperm migration up a gradient of shFF (P < 0.05), possibly due to ‘hyperactivated trapping’. We conclude that the high concentration of progesterone (13.5µM) present in the shFF mixture is likely to be responsible for the biphasic sperm [Ca2+]i influx characteristic of a progesterone stimulus. However, the data obtained from the sperm kinesis and AR experiments leads us to hypothesize that the other steroid hormones present in the shFF mixture exert antagonistic effects on progesterone-mediated physiological responses in human spermatozoa.
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Rafaela, Sayuri Cicalise Takeshita. "Factors regulating steroid hormones in Japanese macaques and orangutans." Kyoto University, 2018. http://hdl.handle.net/2433/232299.
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付記する学位プログラム名: 霊長類学・ワイルドライフサイエンス・リーディング大学院Kyoto University (京都大学)
0048
新制・課程博士
博士(理学)
甲第20964号
理博第4416号
新制||理||1634(附属図書館)
京都大学大学院理学研究科生物科学専攻
(主査)准教授 Michael Alan Huffman, 准教授 足立 幾磨, 教授 友永 雅己
学位規則第4条第1項該当
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Pessina, Monica A. "Modulation of rat vaginal structure by sex steroid hormones." Thesis, Boston University, 2005. https://hdl.handle.net/2144/37168.
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Thesis (Ph.D.)--Boston UniversityPLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
The vagina is a key organ in the peripheral genital arousal response. In animal models, pelvic nerve stimulation increases vaginal wall compliance, blood flow and transudation of fluid. Decreases in ovarian steroids are known to induce structural changes in the vagina, and evidence is mounting that alterations in the hormonal milieu contribute to genital pathophysiology. To date, however, mechanisms by which sex steroids regulate vaginal arousal responses have not been adequately studied. Further, limited data are available on the effects of hormone replacement on tissue morphology, hormone receptor distribution and vaginal innervation. We propose that imbalances in sex steroid hormone levels alter the distribution, expression and actions of steroid receptors and neurotransmitters, leading to structural and functional changes in vaginal tissue and impairment the arousal response. The goal of this study was to assess dynamic changes in vaginal tissue structure with hormone deprivation and administration. Female Sprague-Dawley rats were used as an animal model. Intact animals served as controls. Ovariectomized animals were treated for a two week period with vehicle, estradiol, testosterone, progesterone, or a combination of estradiol plus testosterone or progesterone. To assess changes in vaginal physiology and morphology, physiological and histological techniques were used, including stereological analysis and immunohistochemistry for localization of hormone receptors and various neuronal markers.
2031-01-01
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Schwarcz, Leslie Esther. "Linking steroid hormone and Wnt signaling /." view abstract or download file of text, 2006. http://wwwlib.umi.com/cr/uoregon/fullcit?p3211226.
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Thesis (Ph. D.)--University of Oregon, 2006.Typescript. Includes vita and abstract. Includes bibliographical references (leaves 71-82). Also available for download via the World Wide Web; free to University of Oregon users.
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Ng, Pak-yiu. "Investigation on the differential expression and hormonal regulation of olfactomedin in uterus." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39557765.
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Mukerji, Mark Sanjiv. "The effects of progesterone and related compounds on the contractility of rat vascular smooth muscle." Thesis, Lancaster University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302393.
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Tep-areenan, Patcharin. "Mechanisms of 17β-oestradiol- and testosterone-induced vasorelaxation in rat arteries." Thesis, University of Nottingham, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288091.
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Hobbs-Mallyon, David. "Synthetic studies on tricyclospirodienones." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315042.
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McVie, Alison Jane. "Molecular analysis and physical mapping of the human 3#beta#-hydroxysteroid dehydrogenase #DELTA#5/#DELTA#4 isomerase gene family." Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241750.
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McBride, Martin William. "Molecular analysis of the human 3#beta#-hydroxysteriod dehydrogenase #delta#'5/#delta#'4 isomerase gene family." Thesis, University of Glasgow, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360132.
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Ebner, Martin Johannes. "Molecular characterisation of steroids in the mammalian brain." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248377.
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Chan, Christina M. W. "A study of hormone-regulated mRNA in human breast cancer cells in culture." Thesis, University of Liverpool, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357364.
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Isaksson, Friman Erika. "Hormonal treatments and the breast : effects on sex steroid receptor expression and proliferation /." Stockholm : [Karolinska institutets bibl.], 2002. http://diss.kib.ki.se/2002/91-7349-182-9/.
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Evans, Victoria E., University of Western Sydney, of Science Technology and Environment College, and School of Science. "Modulation of tear lipocalin by phosphodiesterase inhibitors and steroid hormones." THESIS_CSTE_SS_Evans_V.xml, 2001. http://handle.uws.edu.au:8081/1959.7/327.
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This project focussed on a protein called tear lipocalin that is believed to interact with the lipid layer of the tears and to promote tear film stability. By modulating this protein, it was proposed that tear film stability could be increased, hence the modulation of lipocalin was suggested as a goal for dry eye therapy. The effects on tear lipocalin expression of two potential therapies for dry eye, pharmacological stimulation with phosphodiesterase inhibitors and hormonal regulation with aldosterone and oestrogen HRT were investigated. Initially a rabbit model was employed and the rabbit tear protein profile was characterised. Stimulation of rabbit secretion using phosphodiesterase compounds did not alter rabbit tear lipocalin secretion. Clinical trial of phosphodiesterase compounds in humans did not alter tear lipocalin expression but variation in tear lipocalin isoform expression was noted and new isoforms of tear lipocalin were identified. The steroid hormone aldosterone appeared to modulate rabbit lipocalin and low MW protein expression. A clinical trial to examine the effect of gender, menopause and oestrogen hormone replacement therapy (HRT) on lipocalin secretion demonstrated that oestrogen HRT down regulated lipocalin secretion, increased the thickness of the lipid layer and improved symptomatology compared to the untreated menopause group. High levels of tear lipcalin in the menopause group were associated with symptoms of ocular burning, suggesting that increasing tear lipocalin levels might aggravate the ocular symptoms associated with dry eye. In this thesis it was demonstrated that tear lipocalin secretion was modulated by steroid hormones, but did not appear to be modulated by phosphodiesterase inhibitors. Variation in tear lipocalin concentration did not correlate with measures of tear film stability and indeed, high levels of tear lipocalin may be deleterious for dry eye symptoms. Further analysis of tear a stimulants and hormones will lead to better understanding of tear regulation and will open avenues for dry eye therapy. The application of proteomic tools to tear film will advance our understanding of tear film composition and the role each component in tear film stability.Doctor of Philosophy (PhD)
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Boman, Karin. "Endometrial carcinoma : steroid hormones and receptors in relation to proliferation." Doctoral thesis, Umeå universitet, Onkologi, 1993. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-100588.
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The significance of the hormonal milieu for endometrial changes is as well-known as its link with endometrial carcinoma. Unopposed oestradiol treatment is shown to increase the incidence for this cancer. Obesity leads to elevated levels of oestrogens and is a risk factor for endometrial carcinoma. An association between high tumour proliferation and prognosis is a general feature of human cancer. Tumour growth can be expressed as proliferation rate and flow cytometry (FCM) is a sensitive and reproducable method to estimate S-phase fraction (SPF) and ploidy level. Both parameters have been shown to correlate with prognosis. Sex steroid hormone levels were analysed together with clinical parameters, SPF, and receptors in established endometrial carcinoma. The study consisted of postmenopausal women with endometrial adenocarcinoma. H ormones were analysed in 127 patients, 99 were analysed for FCM and 60 for oestrogen and progesterone receptors. RIA technique was used for hormone assay of oestrone, oestradiol, progesterone, androstenedione and testosterone plasma levels. The receptors were analysed with an immunohistochemical method, and SPF and ploidy level by flow cytometry. A wide range of oestrogen concentrations was found. Some patients had levels comparable to fertile women. Strong correlations were found between body mass index, weight and depth of uterine cavity. No relations were found between receptors and SPF, apart from oestrogen- receptor positive tumours having a lower SPF when compared with receptor negative tumours. The influence of oestradiol on tumour proliferation expressed as SPF was ambiguous. SPF was increased with higher oestradiol levels in the group of peri-diploid, well-differentiated tumours, while a negative correlation was found for the peri-diploid, moderately differentiated tumours. The aneuploid and poorly differentiated tumours had a high SPF regardless of oestradiol concentration. The association between progesterone concentration and SPF was of a more general nature. Progesterone above a threshold level was related to a lower SPF in well-differentiated and moderately differentiated tumours. Thus endogenous progesterone seems to play a role in controlling the tumour’s proliferation activity, in contrast to oestradiol, that had a role which did not appear to relate to proliferation activity in any specific direction. The only stimulative association was seen in well-differentiated tumours, but SPF was still below the mean value for all diploid tumours.Diss. (sammanfattning) Umeå : Umeå universitet, 1993, härtill 5 uppsatser.
digitalisering@umu
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Clement, Christophe Yannick. "Responses of a Drosophila cell line to insect steroid hormones." Thesis, University of Exeter, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303913.
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Martins, Ana Catarina Dias. "Effects of sex steroid hormones on sertoli cells metabolic pathways." Master's thesis, Universidade da Beira Interior, 2012. http://hdl.handle.net/10400.6/1126.
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Developing germ cells use lactate, derived from glucose metabolism of Sertoli cells (SCs), as their main energy source. Androgens and estrogens have been implicated in the modulation of testicular cells energy metabolism, particularly in SCs. The goal of the present study was to shed light on the effects of sex steroid hormones on glucose metabolic pathways in rat SCs. The mRNA levels of glucose transporters 1 and 3 (GLUT1 and GLUT3), phosphofructokinase 1 (PFK1) and lactate dehydrogenase chain C (LHD C) were analyzed by RT-PCR, and protein levels of GLUTs, PFK1, LDH and monocarboxylate transporter 4 (MCT4) were analyzed by Western Blot, in enriched primary cultures of immature rat SCs treated with 17β-estradiol (E2) or 5α-dihydrotestosterone (DHT). Our results show that both E2 and DHT downregulated the gene transcript levels of PFK-1, GLUT1 and GLUT3. However, only DHT-treated cells presented a downregulation of LDH C gene transcript levels. Interestingly, the protein levels of these enzymes and transporters remained unaltered except in DHT-treated cells that presented a significant decrease on GLUT1 protein levels evidencing a possible pathway for the regulation of glucose metabolism in SCs by androgens. Taken together, these results demonstrated a relationship between the action of sex steroid hormones and energy metabolism of SCs, providing evidences for the mechanisms by which E2 and DHT exert their function as modulators of rat SCs glucose metabolism.As células germinativas em desenvolvimento utilizam lactato, um produto do metabolismo da glicose das células de Sertoli (SCs), como a principal fonte de energia. O papel dos androgénios e estrogénios na modulação do metabolismo energético das células testiculares tem vindo a ser estudado, particularmente nas SCs. O presente estudo tem como objetivo explorar o efeito de hormonas esteróides sexuais sobre as vias envolvidas no metabolismo da glicose em SCs de ratos. Foram analisados os níveis de mRNA de transportadores de glicose (GLUT1 e GLUT3), fosfofrutoquinase-1 (PFK1) e lactato desidrogenase isoforma C (LHD C) por RT-PCR, e por Western Blot foram analisados os níveis proteicos de GLUTs, PFK-1, LDH e transportador de ácidos monocarboxílicos 4 (MCT4). Foram utilizadas para este estudo culturas primárias de SCs de ratos imaturos tratadas com 17βestradiol (E2) ou 5α -dihidrotestosterona (DHT). Os resultados obtidos demonstram que tanto o E2 como o DHT regulam os níveis de transcrição da PFK1, GLUT1 e GLUT3. No entanto, apenas as células tratadas com DHT apresentam uma diminuição nos níveis de transcrição da LDH C. Curiosamente, os níveis de proteína destas enzimas e transportadores permaneceram inalterados, exceto em células tratadas com DHT que apresentaram uma diminuição significativa nos níveis proteicos de GLUT1, pondo em evidência uma possível via para a regulação do metabolismo da glicose em SCs por androgénios. Em conjunto, estes resultados demonstraram uma relação entre a ação das hormonas esteróides sexuais e metabolismo energético das SCs, facultando novas evidências sobre os mecanismos através dos quais o E2 e a DHT exercem a sua função como moduladores do metabolismo da glicose em SCs de rato.
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Evans, Victoria E. "Modulation of tear lipocalin by phosphodiesterase inhibitors and steroid hormones." Thesis, View thesis, 2001. http://handle.uws.edu.au:8081/1959.7/327.
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This project focussed on a protein called tear lipocalin that is believed to interact with the lipid layer of the tears and to promote tear film stability. By modulating this protein, it was proposed that tear film stability could be increased, hence the modulation of lipocalin was suggested as a goal for dry eye therapy. The effects on tear lipocalin expression of two potential therapies for dry eye, pharmacological stimulation with phosphodiesterase inhibitors and hormonal regulation with aldosterone and oestrogen HRT were investigated. Initially a rabbit model was employed and the rabbit tear protein profile was characterised. Stimulation of rabbit secretion using phosphodiesterase compounds did not alter rabbit tear lipocalin secretion. Clinical trial of phosphodiesterase compounds in humans did not alter tear lipocalin expression but variation in tear lipocalin isoform expression was noted and new isoforms of tear lipocalin were identified. The steroid hormone aldosterone appeared to modulate rabbit lipocalin and low MW protein expression. A clinical trial to examine the effect of gender, menopause and oestrogen hormone replacement therapy (HRT) on lipocalin secretion demonstrated that oestrogen HRT down regulated lipocalin secretion, increased the thickness of the lipid layer and improved symptomatology compared to the untreated menopause group. High levels of tear lipcalin in the menopause group were associated with symptoms of ocular burning, suggesting that increasing tear lipocalin levels might aggravate the ocular symptoms associated with dry eye. In this thesis it was demonstrated that tear lipocalin secretion was modulated by steroid hormones, but did not appear to be modulated by phosphodiesterase inhibitors. Variation in tear lipocalin concentration did not correlate with measures of tear film stability and indeed, high levels of tear lipocalin may be deleterious for dry eye symptoms. Further analysis of tear a stimulants and hormones will lead to better understanding of tear regulation and will open avenues for dry eye therapy. The application of proteomic tools to tear film will advance our understanding of tear film composition and the role each component in tear film stability.
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Evans, Victoria E. "Modulation of tear lipocalin by phosphodiesterase inhibitors and steroid hormones /." View thesis, 2001. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030707.110927/index.html.
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Qazi, Romena. "The role of the urokinase family in invasion by breast cancer." Thesis, University of Glasgow, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266538.
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Chan, Ka-man. "Molecular cloning and characterization of two cDNAs encoding for two forms of FTZ-F1 in the sand shrimp, Metapenaeus ensis /." Hong Kong : University of Hong Kong, 1999. http://sunzi.lib.hku.hk/hkuto/record.jsp?B21021260.
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Yap, Bin Kiat. "Exercise-stress responses of urinary hormones." Thesis, The University of Sydney, 1994. https://hdl.handle.net/2123/26858.
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Knowledge on the effects of episodic or short-term exercise-stress on changes of corticosteroids, androgenic steroids and gonadotropins still remains fragmentary and inconclusive. In this study an alternative approach to investigate these changes, based on the concentration ratios of urinary total testosterone (T), luteinizing hormone (LH), free cortisol(F),cortisone (E) and their primary metabolites tetrahydrocortisol (THF) and tetrahydrocortisone (THE), was developed to profile shortterm exercise-stress responses in healthy, drug-free male athletes and sedentary subjects.
The hormonal concentrations were measured using the sensitive and accurate gas chromatography-mass selective detection (GC MSD) and microparticle enzyme immunoassay (MEIA) analytical techniques which were developed. Stress profiles derived from exercise-stress at V02max, 60-80% VOgmax and 50-55% VOzmax were plotted using the ratios of E/F, THE/F, THE/E, LH/T, LH/F, LH/E, T/F and T/E.
Significant changes between the basal and post-exercise ratios were found to be consistent and more representative of acute changes in stress levels compared to single corticosteroid and testosterone concentrations. Marked changes in LH values were also observed to reflect a dramatic increase in stress— response.
The ratio profiling method was also employed to investigate the urinary hormonal changes of a separate group of athletes who were subjected to mild exercise (50% VOZmax) combined with warm (27°C) and moderate humidity (64%). Variation in temperature and humidity resulted in changes to some of the corticosteroid ratio profiles which were consistent with those found in the earlier trials. In a subsequent field study the swim-stress responses of a group of teenage, male swimmers participating in a typical training session during a competition season were also profiled. Whilst no marked changes were found in the LH and T ratios, some of the corticosteroid ratios showed a trend towards positive significance. The profiles appeared to reflect the level of swim-stress prescribed in the training that was less than an equivalent of treadmill running at 60-80% VOZmax The consistent results obtained from the evaluation of the stress-response profiles showed that the profiling approach based on the proportionate changes of urinary F,E,THF,THE,T and LH levels could be utilised as a reliable method for evaluating the inter-relationship between the various hormones in human stress studies. The method could be of advantage in determining the training status of athletes in competitions and for drug-testing purposes.
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Blewitt, R. W. "Mechanism of action of steroid hormones on human lymphoid cancer cells." Thesis, University of Leeds, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355610.
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Scarano, Wellerson Rodrigo. "Repercussões histopatologicas na prostata ventral do gerbilo da Mongolia (Merinones unguiculatus) apos suplementação por hormonios esteroides." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317918.
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Orientador: Sebastião Roberto TabogaTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O tecido prostático é susceptível aos desníveis hormonais provocados, principalmente pelo processo de envelhecimento. A hiperplasia benigna prostática e o câncer de próstata são doenças que acometem uma grande parcela da população masculina, e parecem estar envolvidas com alterações hormonais. Por isso, o esclarecimento dos processos celulares e teciduais envolvendo os hormônios sexuais: testosterona e estradiol são, sem dúvida, importantes para o entendimento da etiologia desses processos patológicos. O gerbilo (Meriones unguiculatus) foi utilizado como modelo experimental pois, segundo a literatura, é susceptível ao aparecimento de lesões autóctones e responde bem à carcinogênese experimental, mostrando-se um bom modelo experimental. Numa primeira etapa, foram utilizados animais de três idades diferentes: púbere, adulta e senil. Esses animais foram submetidos à suplementação androgênica e as próstatas ventrais foram destinadas a análises histopatológicas, quantitativas, imunocitoquímicas e ultraestruturais. Foi observado aumento no peso da glândula e também na altura das células epiteliais em todas as idades. Tal aumento reflete o aumento da capacidade sintética observada pela dilatação das organelas de síntese, às vezes de aspecto vesiculoso, ocupando toda a região supranuclear. Nos animais adultos e velhos foram notadas regiões hiperplásicas e displásicas freqüentemente associadas a Neoplasias Intraepiteliais de diferentes graus e a adenocarcinomas. Houve aumento na espessura da camada de células musculares lisas (CML) ao redor dos ácinos nos animais púberes e adultos, enquanto nos animais velhos houve diminuição dessa camada. Além disso, as CML se mostraram aparentemente hipertróficas e com maior atividade sintética nos animais púberes e adultos. Foi notado aparente aumento da vascularização periacinar, onde se observou a presença de freqüentes vasos sanguíneos em todas as idades após o tratamento. Ademais, em todas as idades foi observado aumento da densidade de marcação de receptores androgênicos após o tratamento, evidenciando a possível relação desses receptores com os efeitos observados. Em uma segunda etapa experimental, avaliou-se o efeito do estradiol sobre o tecido prostático intacto e hipoandrogênico em animais adultos, tentando com isso simular situações de descompensação hormonal, típicas da senilidade. As alterações epiteliais foram freqüentes nos animais tratados com estradiol onde se observou aumento na altura das células epiteliais, aparecimento de regiões de intensa displasia e hiperplasia, e a formação de PINs. Outro aspecto que independe da presença da testosterona é o arranjo dos elementos fibrilares e não fibrilares da matriz extracelular entre as CML, apontando para um possível papel dessas células no rearranjo e na síntese desses componentes após os tratamentos estrogênicos. Nos animais castrados observou-se acúmulo de elementos da matriz extracelular sob o epitélio e em animais intactos presença desses elementos dispersos e escassos. Em ambos os grupos: intactos e castrados, notou-se que as CML e os fibroblastos apresentam fenótipo secretor acentuado após o tratamento com estradiol. Houve aumento na densidade de marcação ERa e AR positivos em regiões de hiperplasia apontando para um possível papel desses receptores na formação de lesões pré-malígnas e malignas. Portando, conclui-se que o gerbilo é susceptível a ação da testosterona e do estradiol, os quais provocam desarranjos estruturais e ultraestruturais de cunho patológico e funcional, mostrando-se um ótimo modelo para o estudo das doenças prostáticas de etiologia hormonal
Abstract: Not informed.
Doutorado
Biologia Celular
Doutor em Biologia Celular e Estrutural
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Cartinella, Joshua L. "Removal of natural steroid hormones from wastewater using membrane contactor processes." abstract and full text PDF (free order & download UNR users only), 2006. http://0-gateway.proquest.com.innopac.library.unr.edu/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1436019.
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Gorodeski, George Israel. "Retinoids and steroid hormones regulate differentiation of cultured human ectocervical cells." Case Western Reserve University School of Graduate Studies / OhioLINK, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=case1054845951.
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Fang, Min. "Removal of Natural and Synthetic Steroid Hormones through Constructed Wetland Microcosm." University of Toledo / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1292943388.
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Cartinella, Joshua L. "Removal of natural steroid hormones from wastewater using membrane contractor processes." abstract and full text PDF (free order & download UNR users only), 2008. http://0-gateway.proquest.com.innopac.library.unr.edu/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1436019.
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Shields, Tammy S. "Endogenous hormones and the risk of cervical cancer /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/10909.
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Miller, Julie Elizabeth. "Wandering Behavior in Manduca Sexta: Investigating Steroid Hormone Effects on Neural Circuits For Locomotor Behavior." Diss., Tucson, Arizona : University of Arizona, 2005. http://etd.library.arizona.edu/etd/GetFileServlet?file=file:///data1/pdf/etd/azu%5Fetd%5F1020%5F1%5Fm.pdf&type=application/pdf.
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Genade, Tyrone. "Control analysis of adrenal Sseroidogenesis." Thesis, Stellenbosch : University of Stellenbosch, 2004. http://hdl.handle.net/10019.1/2156.
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Thesis (MSc (Biochemistry))--University of Stellenbosch, 2004.This study describes: 1. Investigation of product inhibition regarding the metabolism of progesterone in ovine adrenal micosomes. 2. The employment of novel cell culture techniques to study the effect of CYP17 and CYP21 concentration on adrenal progesterone metabolism. 3. The formulation of a mathematical model describing the behaviour of the observed results in point 2.
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Woodward, Terry L. "Effects of ovarian steroids on bovine mammary epithelial cells : in vitro and in viro evidence of indirect stimulation of proliferation /." Thesis, Virginia Tech, 1991. http://hdl.handle.net/10919/41629.
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Three studies were conducted to determine the effects of ovarian steroids on bovine mammary epithelial cell proliferation. In a first study, estrogen (E), progesterone (P), or E+P were administered to prepubertal beef heifers and biopsied mammary parenchyma taken before and following treatment were compared for growth by evaluation of histoautoradiographic incorporation of thymidine. Estrogen increased epithelial cell growth by 24 h, and fibroblasts to a lesser magnitude by 48-96 h. Estrogen and P was less effective and P was ineffective in increasing proliferation in all cell types studied. Proliferation of adipocytes was not altered.
A second study characterized hormone responsive proliferation of Mac-T cells, a recent clonal bovine mammary epithelial cell strain. Mac-T cells responded to all hormones tested as would be expected in vivo. Additionally, passage, harvesting, quantification, freezing, and co-culture techniques were modified to facilitate uncomplicated, timely, inexpensive, effective testing of growth responsiveness to hormones or growth factors.
In a third study E and P alone, together, with or without serum were unable to increase Mac-T cell proliferation. Serum from prepubertal Holstein heifers after E treatment did not increase growth of Mac-T cells over serum before treatment. Conditioned media from Mac-T or Fib-T (mammary bovine fibroblast cell line) with or without steroids were tested for ability to increase Mac-T cell proliferation. Growth of Mac-T cells was greatest in Fib-T + E conditioned media followed by Fib-T, then Mac-T and lastly fresh media. Steroid exposure did not enhance the ability of Mac-T cell conditioned media to increase Mac-T cell proliferation.
In conclusion, E appears to be the primary ovarian steroid involved in initiating bovine mammogenesis. However, estrogen’s action is not direct and may be caused by paracrine release of growth factors.Master of Science
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Leão, Rogério de Barros Ferreira 1977. "Receptores de estrógeno e progesterona em pólipos endometriais de usuárias e não usuárias de tamoxifeno e no endométrio atrófico." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310481.
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Orientador: Lúcia Helena Simões da Costa PaivaDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: Introdução: O tamoxifeno é utilizado no tratamento do câncer de mama receptor de estrogênio positivo. Seu mecanismo de ação está na inibição do crescimento das células malignas por antagonismo competitivo com estrógenos pelos receptores estrogênicos. A ação do tamoxifeno nestes receptores é variável e, dependendo do tecido, pode ter ação antagonista ou agonista. Em mulheres menopausadas usuárias de tamoxifeno, observa-se uma maior incidência de patologias endometriais, sendo o pólipo endometrial a alteração mais frequente. Sua patogênese não está bem estabelecida, mas parece estar relacionada a fatores hormonais. Objetivos: Comparar a expressão de receptor de estrógeno (RE) e de receptor de progesterona (RP) em pólipos endometriais de usuárias de tamoxifeno com pólipos endometriais e endométrio atrófico de não usuárias na pós-menopausa. Material e métodos: Entre mulheres submetidas à histeroscopia cirúrgica no Hospital da Mulher Prof. Dr. Aristodemo Pinotti - CAISM/UNICAMP de janeiro de 1998 a dezembro de 2008, foram selecionadas 84 mulheres na pós-menopausa usuárias de tamoxifeno, com pólipo endometrial benigno. Esse grupo foi comparado a 84 amostras de endométrio atrófico e 252 pólipos benignos de mulheres na pós-menopausa não usuárias de tamoxifeno e sem antecedente de terapia hormonal. As expressões de RE e RP foram avaliadas através de imuno-histoquímica segundo a porcentagem de células coradas, intensidade da coloração nuclear e escore final. A expressão de RE e RP no epitélio glandular e no estroma dos pólipos de usuárias de tamoxifeno foi comparada com o endométrio atrófico e os pólipos de não usuárias separadamente, utilizando o Teste de Mann-Whitney, corrigido pelo método de Bonferroni, teste exato de Fisher ou Qui-quadrado. Resultados: os pólipos de usuárias de tamoxifeno apresentaram maior expressão de RE e RP no epitélio glandular e estroma, em relação ao endométrio atrófico (p<0,0001). Em relação aos pólipos de não usuárias, as usuárias apresentaram maior expressão de RP no epitélio glandular (p=0,0014) e estroma (p=0,0056), não apresentando diferença significativa em relação ao RE. A maioria dos pólipos das usuárias e não usuárias de tamoxifeno apresentavam RE/RP positivos enquanto a maioria dos endométrios atróficos era RE/RP negativos. Conclusões: Os pólipos apresentam aumento frequente de RE, independentemente do uso do tamoxifeno. Por outro lado, os altos níveis de RP parecem consistentes com os efeitos agonistas da droga
Abstract: Introduction: Tamoxifen has been used for the treatment of strogen receptor-positive breast cancer. The effect of tamoxifen in breast cancer is the it inhibition cancer cell growth by competitive antagonism with strogen for strogen receptor (ER). The mechanism of action of tamoxifen in this receptor varies among different tissues, with antagonist effect (e.g. in breast) ou agonist (e.g. in endometrium). Thus, in menopausal women who use tamoxifen, a higher incidence of endometrial alterations is observed and endometrial polyps are the most common. The pathophysiology of endometrial polyp is still not definitely established but it seems to be related to hormone influence. Objectives: To compare the expression of estrogen receptors (ER) and progesterone receptors (PR) in endometrial polyps of tamoxifen users to atrophic endometrium and endometrial polyps of postmenopausal nonusers of tamoxifen. Material and methods: Among women undergoing surgical hysteroscopy in Hospital da Mulher Prof. Dr. Aristodemo Pinotti - CAISM/UNICAMP, from January 1998 to December 2008, 84 tamoxifen users with benign endometrial polyp were selected. This group was compared to 84 samples of atrophic endometrium and 252 benign polyps of postmenopausal women who were non-users of tamoxifen and no previous history of hormone therapy use. ER/PR expression was assessed by immunohistochemistry study according to the percentage of stained cells, intensity of nuclear color and final score. The expression ER and PR in the glandular epithelium and stroma of polyp tissue from tamoxifen users was compared to atrophic endometrium and polyps from non-users separately, using the Mann-Whitney test corrected by the Bonferroni method, Fisher's exct test or Chi-square test. Results: Polyps of tamoxifen users had a higher expression of ER and PR in the glandular epithelium and stroma, in relation to the atrophic endometrium (p<0.0001). Regarding polyps of women not treated with tamoxifen, users had a higher PR expression in the epithelium (p=0.0014) and stroma (p=0.0056), without any difference in ER expression. Most of polipys expressed ER/PR positives while atrophic endometrium were ER/PR negatives. Conclusions: Polyps frequently exhibit increase in ER expression, independent of the use of tamoxifen. High levels of PR seem to be consistent with agonist effects of the drug
Mestrado
Fisiopatologia Ginecológica
Mestre em Ciências da Saúde
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Santana, Luís Carlos Leal. "Impacto de andrógenos sobre a proliferação e atividade de fibroblastos e células epiteliais em cultura celular /." Araraquara, 2016. http://hdl.handle.net/11449/138766.
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Orientador: Luis Carlos SpolidórioResumo: Hormônios esteroides sexuais participam de diversos eventos celulares e moleculares, e exercem influência sobre o epitélio e tecido conjuntivo do periodonto. A testosterona (T), principal hormônio androgênico, pode ser convertida em estradiol (E2) pela ação da enzima aromatase, ou em di-hidrotestosterona (DHT) pela ação da enzima 5α-redutase. Para elucidar o impacto de andrógenos sobre as células que compõem os tecidos conjuntivo e epitelial, fibroblastos e queratinócitos foram avaliados em relação aos efeitos de diferentes concentrações de T e DHT, além da exposição ao anastrozol (ANA), flutamida (FLU), fulvestranto (FUL), e às associações farmacológicas T+ANA, T+FLU e T+FUL. Os resultados do presente estudo indicaram que, de modo geral, hormônios esteroides androgênicos exercem efeitos opostos sobre eventos celulares de fibroblastos gengivais humano e células epiteliais HaCaT em cultura celular. Enquanto a T e a DHT agem promovendo o aumento da proliferação e atividade de fibroblastos, a exposição de células HaCaT a estes mesmos andrógenos resulta em inibição ou exiguidade do crescimento celular, atividade metabólica ou a capacidade de repovoamento da área de arranhão in vitro. Além disso, o tratamento farmacológico com ANA, FLU, FUL, e suas respectivas associações à T, parece influenciar eventos celulares de fibroblastos gengivais humano e células epiteliais HaCaT in vitro.
Abstract: Sex steroid hormones take part in different cellular and molecular process and exert their functions on the epithelium and connective tissue of the periodontium. Testosterone (T), the main androgenic hormone can be converted to estradiol (E2) through the aromatase enzyme action, or into dihydrotestosterone (DHT) by 5α-reductase activity. To elucidate the impact of androgens on the cells that constitute the connective and epithelial tissues, fibroblasts and keratinocytes were evaluated under the effects of different concentrations of T and DHT, besides to be both exposed to anastrozole (ANA), flutamide (FLU), fulvestrant (FUL), and the pharmacological associations T+ANA, T+FLU and T+FUL. The results of this study indicated that, in general, androgenic steroid hormones exert opposite effects on cellular events of human gingival fibroblasts and epithelial cells. While androgens act stimulating gingival fibroblasts, in HaCaT cells androgens promotes a shortage or inhibition of cell growth and activity. Furthermore, pharmacological treatment with ANA, FLU, FUL, and their associations to T, appears to influence cellular events of human gingival fibroblasts and HaCaT cells in vitro.
Mestre
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Hazelett, Dennis J. "Gene expression during the segment-specific death of a muscle during insect metamorphosis /." view abstract or download file of text, 2005. http://wwwlib.umi.com/cr/uoregon/fullcit?p3164079.
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Thesis (Ph. D.)--University of Oregon, 2005.Typescript. Includes vita and abstract. Includes bibliographical references (leaves 118-133). Also available for download via the World Wide Web; free to University of Oregon users.
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Hatthachote, Panadda. "Preparation of human myometrium for term : the role of signalling associated proteins." Thesis, University of Newcastle upon Tyne, 1999. http://hdl.handle.net/10443/518.
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