Dissertations / Theses on the topic 'Steroid hormones'
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Lund-Pero, Margaretha. "Nonspecific esterases in human tissues evidence for their involvement in steroid metabolism and in carcinogenesis /." Lund : Dept. of Molecular Ecogenetics, the Wallenberg Laboratory, University of Lund, 1995. http://catalog.hathitrust.org/api/volumes/oclc/39781861.html.
Full textSeymour, Beverley Lesley. "The effect of steroid hormones on the size of myometrial cells : a morphometric study." Thesis, Cape Technikon, 1997. http://hdl.handle.net/20.500.11838/1503.
Full textThe aims of this study were to measure: 1. Myometrial cells of menopausal uteri to establish whether they atrophy after the menopause. 2. Myometrial cells at different phases of the menstrual cycle to investigate the influences of oestrogen and progesterone during the cycle. 3. Myometrial cells in the fundus and lower uterine segment to establish whether they differ in size. 4. Myometrial cells of pregnant uteri to investigate the effect of the hormonal status of pregnant women on the size of myometrial cells. 5. Neoplastic cells of leiomyomas of the uterus to investigate whether these benign tumours behave in the same manner as myometrium or, because they are neoplastic, they react differently. A preliminary investigation was undertaken to establish the optimal methodology for this study to measure myometrial and leiomyoma nuclei in the uterus. The aims of this preliminary investigation were: 1. To test the reproducibility of measurements of myometrial and leiomyoma nuclei in transverse and cross section. 2. To test five histological staining methods to ascertain the best method for a morphometric study on uterine cells. 3. To find the minimum sample size of nuclei per section of myometrium or leiomyoma in order to yield statistically significant results. This preliminary study found that the Haematoxylin and Eosin stain gave the most statistically reproducible measurements. Subjective assessment of the five staining methods also found Haematoxylin and Eosin to be optimal. It was also found during the preliminary study that measuring the myometrial nuclei in cross rather than transverse section gave the most statistically reproducible measurements. It was also found that it was best to use an axial ratio criterion of 0,9 when measuring cross-sectioned myometrial nuclei. The optimum sample size per section was also investigated and it was found that measuring 100 nuclei was optimal. It was found that in the uteri used in this study there was no statistically significant decrease in nuclear size after the menopause. It was also found that there was no statistically significant difference in nuclear size during the different phases of the menstrual cycle. There was also no notable difference in nuclear size between nuclei in the fundus and lower segment of the uteri in this study. It was found that there was a significant increase in the size of nuclei in leiomyomas compared to the normal myometrial nuclei from the same patient. The myometrial nuclei from pregnant uteri were also significantly larger than those from non-gravid uteri.
Al-Mana, D. "Ovarian steroid hormones and auditory function." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1386639/.
Full textFarquharson, Roy G. "Fetal abstraction of placental steroid hormones." Thesis, University of Aberdeen, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328653.
Full textGingnell, Malin. "Ovarian Steroid Hormones, Emotion Processing and Mood." Doctoral thesis, Uppsala universitet, Obstetrik & gynekologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-199791.
Full textMurai, Takahiro. "Study on the diglucuronidation reaction of steroid hormones." Kyoto University, 2008. http://hdl.handle.net/2433/136704.
Full textCharlton, Michael Hugh. "Theoretical studies of steroid hormones and related compounds." Thesis, University of St Andrews, 1992. http://hdl.handle.net/10023/14449.
Full textMercurio, S. "ROLE OF STEROID HORMONES IN ECHINOID REPRODUCTIVE BIOLOGY." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/230752.
Full textNawaz, Zafar. "Molecular Mechanism of Action of Steroid Hormone Receptors." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc798398/.
Full textFischer, Katharina. "The mineralocorticoid receptor amino terminal transactivation domain investigation of structural plasticity and protein-protein interactions /." Thesis, Available from the University of Aberdeen Library & Historic Collections Digital Resources, 2008. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24694.
Full textTitle from web page (viewed on Feb. 23, 2009). With: Natural disordered sequences in the amino terminal domain of nuclear receptors : lessons from the androgen and glucocorticoid receptors / Iain J. McEwan ... et al. Nuclear Receptor Signalling. 2007: 5. Includes bibliographical references.
Ildgruben, Anna. "Human vaginal epithelial immunity and influences of hormonal contraceptive usage." Doctoral thesis, Umeå : Klinisk mikrobiologi, enh. för Immunologi och Klin. vetenskap, obstetrik och gynekologi, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-595.
Full textRoberts, April M. "Steroid hormone treatments alter growth characteristics in transformed human ovarian cell lines." Virtual Press, 2003. http://liblink.bsu.edu/uhtbin/catkey/1265095.
Full textFahrbach, Michael. "Anaerobic degradation of steroid hormones by novel denitrifying bacteria." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=982986769.
Full textJones, David G. L. "Regulation of chick fibronectin mRNA levels by steroid hormones." Thesis, University of Manchester, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.277354.
Full textTaiwo, Benjamin Gbenro. "Modulation of human sperm by follicular fluid steroid hormones." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7292/.
Full textRafaela, Sayuri Cicalise Takeshita. "Factors regulating steroid hormones in Japanese macaques and orangutans." Kyoto University, 2018. http://hdl.handle.net/2433/232299.
Full textKyoto University (京都大学)
0048
新制・課程博士
博士(理学)
甲第20964号
理博第4416号
新制||理||1634(附属図書館)
京都大学大学院理学研究科生物科学専攻
(主査)准教授 Michael Alan Huffman, 准教授 足立 幾磨, 教授 友永 雅己
学位規則第4条第1項該当
Pessina, Monica A. "Modulation of rat vaginal structure by sex steroid hormones." Thesis, Boston University, 2005. https://hdl.handle.net/2144/37168.
Full textPLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
The vagina is a key organ in the peripheral genital arousal response. In animal models, pelvic nerve stimulation increases vaginal wall compliance, blood flow and transudation of fluid. Decreases in ovarian steroids are known to induce structural changes in the vagina, and evidence is mounting that alterations in the hormonal milieu contribute to genital pathophysiology. To date, however, mechanisms by which sex steroids regulate vaginal arousal responses have not been adequately studied. Further, limited data are available on the effects of hormone replacement on tissue morphology, hormone receptor distribution and vaginal innervation. We propose that imbalances in sex steroid hormone levels alter the distribution, expression and actions of steroid receptors and neurotransmitters, leading to structural and functional changes in vaginal tissue and impairment the arousal response. The goal of this study was to assess dynamic changes in vaginal tissue structure with hormone deprivation and administration. Female Sprague-Dawley rats were used as an animal model. Intact animals served as controls. Ovariectomized animals were treated for a two week period with vehicle, estradiol, testosterone, progesterone, or a combination of estradiol plus testosterone or progesterone. To assess changes in vaginal physiology and morphology, physiological and histological techniques were used, including stereological analysis and immunohistochemistry for localization of hormone receptors and various neuronal markers.
2031-01-01
Schwarcz, Leslie Esther. "Linking steroid hormone and Wnt signaling /." view abstract or download file of text, 2006. http://wwwlib.umi.com/cr/uoregon/fullcit?p3211226.
Full textTypescript. Includes vita and abstract. Includes bibliographical references (leaves 71-82). Also available for download via the World Wide Web; free to University of Oregon users.
Ng, Pak-yiu. "Investigation on the differential expression and hormonal regulation of olfactomedin in uterus." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39557765.
Full textMukerji, Mark Sanjiv. "The effects of progesterone and related compounds on the contractility of rat vascular smooth muscle." Thesis, Lancaster University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302393.
Full textTep-areenan, Patcharin. "Mechanisms of 17β-oestradiol- and testosterone-induced vasorelaxation in rat arteries." Thesis, University of Nottingham, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288091.
Full textHobbs-Mallyon, David. "Synthetic studies on tricyclospirodienones." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315042.
Full textMcVie, Alison Jane. "Molecular analysis and physical mapping of the human 3#beta#-hydroxysteroid dehydrogenase #DELTA#5/#DELTA#4 isomerase gene family." Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241750.
Full textMcBride, Martin William. "Molecular analysis of the human 3#beta#-hydroxysteriod dehydrogenase #delta#'5/#delta#'4 isomerase gene family." Thesis, University of Glasgow, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360132.
Full textEbner, Martin Johannes. "Molecular characterisation of steroids in the mammalian brain." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248377.
Full textChan, Christina M. W. "A study of hormone-regulated mRNA in human breast cancer cells in culture." Thesis, University of Liverpool, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357364.
Full textIsaksson, Friman Erika. "Hormonal treatments and the breast : effects on sex steroid receptor expression and proliferation /." Stockholm : [Karolinska institutets bibl.], 2002. http://diss.kib.ki.se/2002/91-7349-182-9/.
Full textEvans, Victoria E., University of Western Sydney, of Science Technology and Environment College, and School of Science. "Modulation of tear lipocalin by phosphodiesterase inhibitors and steroid hormones." THESIS_CSTE_SS_Evans_V.xml, 2001. http://handle.uws.edu.au:8081/1959.7/327.
Full textDoctor of Philosophy (PhD)
Boman, Karin. "Endometrial carcinoma : steroid hormones and receptors in relation to proliferation." Doctoral thesis, Umeå universitet, Onkologi, 1993. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-100588.
Full textDiss. (sammanfattning) Umeå : Umeå universitet, 1993, härtill 5 uppsatser.
digitalisering@umu
Clement, Christophe Yannick. "Responses of a Drosophila cell line to insect steroid hormones." Thesis, University of Exeter, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303913.
Full textMartins, Ana Catarina Dias. "Effects of sex steroid hormones on sertoli cells metabolic pathways." Master's thesis, Universidade da Beira Interior, 2012. http://hdl.handle.net/10400.6/1126.
Full textAs células germinativas em desenvolvimento utilizam lactato, um produto do metabolismo da glicose das células de Sertoli (SCs), como a principal fonte de energia. O papel dos androgénios e estrogénios na modulação do metabolismo energético das células testiculares tem vindo a ser estudado, particularmente nas SCs. O presente estudo tem como objetivo explorar o efeito de hormonas esteróides sexuais sobre as vias envolvidas no metabolismo da glicose em SCs de ratos. Foram analisados os níveis de mRNA de transportadores de glicose (GLUT1 e GLUT3), fosfofrutoquinase-1 (PFK1) e lactato desidrogenase isoforma C (LHD C) por RT-PCR, e por Western Blot foram analisados os níveis proteicos de GLUTs, PFK-1, LDH e transportador de ácidos monocarboxílicos 4 (MCT4). Foram utilizadas para este estudo culturas primárias de SCs de ratos imaturos tratadas com 17βestradiol (E2) ou 5α -dihidrotestosterona (DHT). Os resultados obtidos demonstram que tanto o E2 como o DHT regulam os níveis de transcrição da PFK1, GLUT1 e GLUT3. No entanto, apenas as células tratadas com DHT apresentam uma diminuição nos níveis de transcrição da LDH C. Curiosamente, os níveis de proteína destas enzimas e transportadores permaneceram inalterados, exceto em células tratadas com DHT que apresentaram uma diminuição significativa nos níveis proteicos de GLUT1, pondo em evidência uma possível via para a regulação do metabolismo da glicose em SCs por androgénios. Em conjunto, estes resultados demonstraram uma relação entre a ação das hormonas esteróides sexuais e metabolismo energético das SCs, facultando novas evidências sobre os mecanismos através dos quais o E2 e a DHT exercem a sua função como moduladores do metabolismo da glicose em SCs de rato.
Evans, Victoria E. "Modulation of tear lipocalin by phosphodiesterase inhibitors and steroid hormones." Thesis, View thesis, 2001. http://handle.uws.edu.au:8081/1959.7/327.
Full textEvans, Victoria E. "Modulation of tear lipocalin by phosphodiesterase inhibitors and steroid hormones /." View thesis, 2001. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030707.110927/index.html.
Full textQazi, Romena. "The role of the urokinase family in invasion by breast cancer." Thesis, University of Glasgow, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266538.
Full textChan, Ka-man. "Molecular cloning and characterization of two cDNAs encoding for two forms of FTZ-F1 in the sand shrimp, Metapenaeus ensis /." Hong Kong : University of Hong Kong, 1999. http://sunzi.lib.hku.hk/hkuto/record.jsp?B21021260.
Full textYap, Bin Kiat. "Exercise-stress responses of urinary hormones." Thesis, The University of Sydney, 1994. https://hdl.handle.net/2123/26858.
Full textBlewitt, R. W. "Mechanism of action of steroid hormones on human lymphoid cancer cells." Thesis, University of Leeds, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355610.
Full textScarano, Wellerson Rodrigo. "Repercussões histopatologicas na prostata ventral do gerbilo da Mongolia (Merinones unguiculatus) apos suplementação por hormonios esteroides." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317918.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O tecido prostático é susceptível aos desníveis hormonais provocados, principalmente pelo processo de envelhecimento. A hiperplasia benigna prostática e o câncer de próstata são doenças que acometem uma grande parcela da população masculina, e parecem estar envolvidas com alterações hormonais. Por isso, o esclarecimento dos processos celulares e teciduais envolvendo os hormônios sexuais: testosterona e estradiol são, sem dúvida, importantes para o entendimento da etiologia desses processos patológicos. O gerbilo (Meriones unguiculatus) foi utilizado como modelo experimental pois, segundo a literatura, é susceptível ao aparecimento de lesões autóctones e responde bem à carcinogênese experimental, mostrando-se um bom modelo experimental. Numa primeira etapa, foram utilizados animais de três idades diferentes: púbere, adulta e senil. Esses animais foram submetidos à suplementação androgênica e as próstatas ventrais foram destinadas a análises histopatológicas, quantitativas, imunocitoquímicas e ultraestruturais. Foi observado aumento no peso da glândula e também na altura das células epiteliais em todas as idades. Tal aumento reflete o aumento da capacidade sintética observada pela dilatação das organelas de síntese, às vezes de aspecto vesiculoso, ocupando toda a região supranuclear. Nos animais adultos e velhos foram notadas regiões hiperplásicas e displásicas freqüentemente associadas a Neoplasias Intraepiteliais de diferentes graus e a adenocarcinomas. Houve aumento na espessura da camada de células musculares lisas (CML) ao redor dos ácinos nos animais púberes e adultos, enquanto nos animais velhos houve diminuição dessa camada. Além disso, as CML se mostraram aparentemente hipertróficas e com maior atividade sintética nos animais púberes e adultos. Foi notado aparente aumento da vascularização periacinar, onde se observou a presença de freqüentes vasos sanguíneos em todas as idades após o tratamento. Ademais, em todas as idades foi observado aumento da densidade de marcação de receptores androgênicos após o tratamento, evidenciando a possível relação desses receptores com os efeitos observados. Em uma segunda etapa experimental, avaliou-se o efeito do estradiol sobre o tecido prostático intacto e hipoandrogênico em animais adultos, tentando com isso simular situações de descompensação hormonal, típicas da senilidade. As alterações epiteliais foram freqüentes nos animais tratados com estradiol onde se observou aumento na altura das células epiteliais, aparecimento de regiões de intensa displasia e hiperplasia, e a formação de PINs. Outro aspecto que independe da presença da testosterona é o arranjo dos elementos fibrilares e não fibrilares da matriz extracelular entre as CML, apontando para um possível papel dessas células no rearranjo e na síntese desses componentes após os tratamentos estrogênicos. Nos animais castrados observou-se acúmulo de elementos da matriz extracelular sob o epitélio e em animais intactos presença desses elementos dispersos e escassos. Em ambos os grupos: intactos e castrados, notou-se que as CML e os fibroblastos apresentam fenótipo secretor acentuado após o tratamento com estradiol. Houve aumento na densidade de marcação ERa e AR positivos em regiões de hiperplasia apontando para um possível papel desses receptores na formação de lesões pré-malígnas e malignas. Portando, conclui-se que o gerbilo é susceptível a ação da testosterona e do estradiol, os quais provocam desarranjos estruturais e ultraestruturais de cunho patológico e funcional, mostrando-se um ótimo modelo para o estudo das doenças prostáticas de etiologia hormonal
Abstract: Not informed.
Doutorado
Biologia Celular
Doutor em Biologia Celular e Estrutural
Cartinella, Joshua L. "Removal of natural steroid hormones from wastewater using membrane contactor processes." abstract and full text PDF (free order & download UNR users only), 2006. http://0-gateway.proquest.com.innopac.library.unr.edu/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1436019.
Full textGorodeski, George Israel. "Retinoids and steroid hormones regulate differentiation of cultured human ectocervical cells." Case Western Reserve University School of Graduate Studies / OhioLINK, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=case1054845951.
Full textFang, Min. "Removal of Natural and Synthetic Steroid Hormones through Constructed Wetland Microcosm." University of Toledo / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1292943388.
Full textCartinella, Joshua L. "Removal of natural steroid hormones from wastewater using membrane contractor processes." abstract and full text PDF (free order & download UNR users only), 2008. http://0-gateway.proquest.com.innopac.library.unr.edu/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1436019.
Full textShields, Tammy S. "Endogenous hormones and the risk of cervical cancer /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/10909.
Full textMiller, Julie Elizabeth. "Wandering Behavior in Manduca Sexta: Investigating Steroid Hormone Effects on Neural Circuits For Locomotor Behavior." Diss., Tucson, Arizona : University of Arizona, 2005. http://etd.library.arizona.edu/etd/GetFileServlet?file=file:///data1/pdf/etd/azu%5Fetd%5F1020%5F1%5Fm.pdf&type=application/pdf.
Full textGenade, Tyrone. "Control analysis of adrenal Sseroidogenesis." Thesis, Stellenbosch : University of Stellenbosch, 2004. http://hdl.handle.net/10019.1/2156.
Full textThis study describes: 1. Investigation of product inhibition regarding the metabolism of progesterone in ovine adrenal micosomes. 2. The employment of novel cell culture techniques to study the effect of CYP17 and CYP21 concentration on adrenal progesterone metabolism. 3. The formulation of a mathematical model describing the behaviour of the observed results in point 2.
Woodward, Terry L. "Effects of ovarian steroids on bovine mammary epithelial cells : in vitro and in viro evidence of indirect stimulation of proliferation /." Thesis, Virginia Tech, 1991. http://hdl.handle.net/10919/41629.
Full textMaster of Science
Leão, Rogério de Barros Ferreira 1977. "Receptores de estrógeno e progesterona em pólipos endometriais de usuárias e não usuárias de tamoxifeno e no endométrio atrófico." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310481.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: Introdução: O tamoxifeno é utilizado no tratamento do câncer de mama receptor de estrogênio positivo. Seu mecanismo de ação está na inibição do crescimento das células malignas por antagonismo competitivo com estrógenos pelos receptores estrogênicos. A ação do tamoxifeno nestes receptores é variável e, dependendo do tecido, pode ter ação antagonista ou agonista. Em mulheres menopausadas usuárias de tamoxifeno, observa-se uma maior incidência de patologias endometriais, sendo o pólipo endometrial a alteração mais frequente. Sua patogênese não está bem estabelecida, mas parece estar relacionada a fatores hormonais. Objetivos: Comparar a expressão de receptor de estrógeno (RE) e de receptor de progesterona (RP) em pólipos endometriais de usuárias de tamoxifeno com pólipos endometriais e endométrio atrófico de não usuárias na pós-menopausa. Material e métodos: Entre mulheres submetidas à histeroscopia cirúrgica no Hospital da Mulher Prof. Dr. Aristodemo Pinotti - CAISM/UNICAMP de janeiro de 1998 a dezembro de 2008, foram selecionadas 84 mulheres na pós-menopausa usuárias de tamoxifeno, com pólipo endometrial benigno. Esse grupo foi comparado a 84 amostras de endométrio atrófico e 252 pólipos benignos de mulheres na pós-menopausa não usuárias de tamoxifeno e sem antecedente de terapia hormonal. As expressões de RE e RP foram avaliadas através de imuno-histoquímica segundo a porcentagem de células coradas, intensidade da coloração nuclear e escore final. A expressão de RE e RP no epitélio glandular e no estroma dos pólipos de usuárias de tamoxifeno foi comparada com o endométrio atrófico e os pólipos de não usuárias separadamente, utilizando o Teste de Mann-Whitney, corrigido pelo método de Bonferroni, teste exato de Fisher ou Qui-quadrado. Resultados: os pólipos de usuárias de tamoxifeno apresentaram maior expressão de RE e RP no epitélio glandular e estroma, em relação ao endométrio atrófico (p<0,0001). Em relação aos pólipos de não usuárias, as usuárias apresentaram maior expressão de RP no epitélio glandular (p=0,0014) e estroma (p=0,0056), não apresentando diferença significativa em relação ao RE. A maioria dos pólipos das usuárias e não usuárias de tamoxifeno apresentavam RE/RP positivos enquanto a maioria dos endométrios atróficos era RE/RP negativos. Conclusões: Os pólipos apresentam aumento frequente de RE, independentemente do uso do tamoxifeno. Por outro lado, os altos níveis de RP parecem consistentes com os efeitos agonistas da droga
Abstract: Introduction: Tamoxifen has been used for the treatment of strogen receptor-positive breast cancer. The effect of tamoxifen in breast cancer is the it inhibition cancer cell growth by competitive antagonism with strogen for strogen receptor (ER). The mechanism of action of tamoxifen in this receptor varies among different tissues, with antagonist effect (e.g. in breast) ou agonist (e.g. in endometrium). Thus, in menopausal women who use tamoxifen, a higher incidence of endometrial alterations is observed and endometrial polyps are the most common. The pathophysiology of endometrial polyp is still not definitely established but it seems to be related to hormone influence. Objectives: To compare the expression of estrogen receptors (ER) and progesterone receptors (PR) in endometrial polyps of tamoxifen users to atrophic endometrium and endometrial polyps of postmenopausal nonusers of tamoxifen. Material and methods: Among women undergoing surgical hysteroscopy in Hospital da Mulher Prof. Dr. Aristodemo Pinotti - CAISM/UNICAMP, from January 1998 to December 2008, 84 tamoxifen users with benign endometrial polyp were selected. This group was compared to 84 samples of atrophic endometrium and 252 benign polyps of postmenopausal women who were non-users of tamoxifen and no previous history of hormone therapy use. ER/PR expression was assessed by immunohistochemistry study according to the percentage of stained cells, intensity of nuclear color and final score. The expression ER and PR in the glandular epithelium and stroma of polyp tissue from tamoxifen users was compared to atrophic endometrium and polyps from non-users separately, using the Mann-Whitney test corrected by the Bonferroni method, Fisher's exct test or Chi-square test. Results: Polyps of tamoxifen users had a higher expression of ER and PR in the glandular epithelium and stroma, in relation to the atrophic endometrium (p<0.0001). Regarding polyps of women not treated with tamoxifen, users had a higher PR expression in the epithelium (p=0.0014) and stroma (p=0.0056), without any difference in ER expression. Most of polipys expressed ER/PR positives while atrophic endometrium were ER/PR negatives. Conclusions: Polyps frequently exhibit increase in ER expression, independent of the use of tamoxifen. High levels of PR seem to be consistent with agonist effects of the drug
Mestrado
Fisiopatologia Ginecológica
Mestre em Ciências da Saúde
Santana, Luís Carlos Leal. "Impacto de andrógenos sobre a proliferação e atividade de fibroblastos e células epiteliais em cultura celular /." Araraquara, 2016. http://hdl.handle.net/11449/138766.
Full textResumo: Hormônios esteroides sexuais participam de diversos eventos celulares e moleculares, e exercem influência sobre o epitélio e tecido conjuntivo do periodonto. A testosterona (T), principal hormônio androgênico, pode ser convertida em estradiol (E2) pela ação da enzima aromatase, ou em di-hidrotestosterona (DHT) pela ação da enzima 5α-redutase. Para elucidar o impacto de andrógenos sobre as células que compõem os tecidos conjuntivo e epitelial, fibroblastos e queratinócitos foram avaliados em relação aos efeitos de diferentes concentrações de T e DHT, além da exposição ao anastrozol (ANA), flutamida (FLU), fulvestranto (FUL), e às associações farmacológicas T+ANA, T+FLU e T+FUL. Os resultados do presente estudo indicaram que, de modo geral, hormônios esteroides androgênicos exercem efeitos opostos sobre eventos celulares de fibroblastos gengivais humano e células epiteliais HaCaT em cultura celular. Enquanto a T e a DHT agem promovendo o aumento da proliferação e atividade de fibroblastos, a exposição de células HaCaT a estes mesmos andrógenos resulta em inibição ou exiguidade do crescimento celular, atividade metabólica ou a capacidade de repovoamento da área de arranhão in vitro. Além disso, o tratamento farmacológico com ANA, FLU, FUL, e suas respectivas associações à T, parece influenciar eventos celulares de fibroblastos gengivais humano e células epiteliais HaCaT in vitro.
Abstract: Sex steroid hormones take part in different cellular and molecular process and exert their functions on the epithelium and connective tissue of the periodontium. Testosterone (T), the main androgenic hormone can be converted to estradiol (E2) through the aromatase enzyme action, or into dihydrotestosterone (DHT) by 5α-reductase activity. To elucidate the impact of androgens on the cells that constitute the connective and epithelial tissues, fibroblasts and keratinocytes were evaluated under the effects of different concentrations of T and DHT, besides to be both exposed to anastrozole (ANA), flutamide (FLU), fulvestrant (FUL), and the pharmacological associations T+ANA, T+FLU and T+FUL. The results of this study indicated that, in general, androgenic steroid hormones exert opposite effects on cellular events of human gingival fibroblasts and epithelial cells. While androgens act stimulating gingival fibroblasts, in HaCaT cells androgens promotes a shortage or inhibition of cell growth and activity. Furthermore, pharmacological treatment with ANA, FLU, FUL, and their associations to T, appears to influence cellular events of human gingival fibroblasts and HaCaT cells in vitro.
Mestre
Hazelett, Dennis J. "Gene expression during the segment-specific death of a muscle during insect metamorphosis /." view abstract or download file of text, 2005. http://wwwlib.umi.com/cr/uoregon/fullcit?p3164079.
Full textTypescript. Includes vita and abstract. Includes bibliographical references (leaves 118-133). Also available for download via the World Wide Web; free to University of Oregon users.
Hatthachote, Panadda. "Preparation of human myometrium for term : the role of signalling associated proteins." Thesis, University of Newcastle upon Tyne, 1999. http://hdl.handle.net/10443/518.
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