Academic literature on the topic 'Staphylococcus pseudintermediu'
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Journal articles on the topic "Staphylococcus pseudintermediu"
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Sleiniute, Jurate, and Jurate Siugzdaite. "Distribution of coagulase-positive staphylococci in humans and dogs." Acta Veterinaria Brno 84, no. 4 (2015): 313–20. http://dx.doi.org/10.2754/avb201584040313.
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Staphylococci form part of the normal flora of humans and a wide variety of animals. Some staphylococcal species also cause infections for human and household pets. The aim of this study was to investigate the prevalence of coagulase-positive staphylococci in humans and dogs within a common household. Coagulase-positive staphylococci could not be identified phenotypically in all cases. Molecular methods were used for the correct identification of pathogenic staphylococci. The prevalence of Staphylococcus aureus was identified in 26/72 (36.1%) dog owners and in 4/61 (6.6%) dogs. Staphylococcus pseudintermedius was isolated from three (4.2%) humans, and from 28 dogs (45.9%). Other coagulase-positive staphylococci species were not isolated in this study. Colonization of S. aureus in humans was significantly higher than in dogs (P < 0.001). Staphylococcus pseudintermedius was more common in dogs compared to humans (P < 0.001). Staphylococcus pseudintermedius strains were isolated from dogs with clinical symptoms more frequently compared to healthy dogs (P = 0.02). Colonization of S. aureus in female dogs was significantly higher compared to male dogs (P = 0.045). The same staphylococci species were isolated from the dog and its owner in six households (12%, 6/50).
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Miszczak, Marta, Sonia Lachowska, and Karolina Bierowiec. "Staphylococcus pseudintermedius: Is it a real threat to human health?" Postępy Higieny i Medycyny Doświadczalnej 75, no. 1 (January 1, 2021): 980–86. http://dx.doi.org/10.2478/ahem-2021-0029.
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Abstract Staphylococcus Intermedius Group (SIG) staphylococci, especially Staphylococcus pseudintermedius (S. pseudintermedius), share many features with the common human Staphylococcus aureus. The similarities concern both the phenotypic characteristics and virulence of the bacteria. It is a cause of difficulties in identifying the species of isolated staphylococci. Until now, S. pseudintermedius was considered a typically animal species, of marginal importance for humans. However, it is likely that the incidence of this staphylococcus in humans is underestimated due to the misidentification of S. pseudintermedius strains as S. aureus. The cases of infections caused by S. pseudintermedius both in humans and animals described so far in the literature show that these bacteria have a similar pathogenic potential. S. pseudintermedius also produces virulence factors that favor colonization of various body regions and infections, and may affect the species composition of the natural microbiota and the host’s immune response mechanisms. Also, S. pseudintermedius may show the ability to grow in the form of a biofilm, which significantly impedes effective antibiotic therapy in clinical practice. Due to its zoonotic potential, S. pseudintermedius deserves the attention of physicians and animal owners.
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Devriese, Luc A., Marc Vancanneyt, Margo Baele, Mario Vaneechoutte, Evelyne De Graef, Cindy Snauwaert, Ilse Cleenwerck, et al. "Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals." International Journal of Systematic and Evolutionary Microbiology 55, no. 4 (July 1, 2005): 1569–73. http://dx.doi.org/10.1099/ijs.0.63413-0.
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Four staphylococcal isolates from clinical and necropsy specimens from a cat, a dog, a horse and a parrot (Psittacus erithacus timneh) were found to constitute a distinct taxon. 16S rRNA gene sequence analysis revealed that its closest phylogenetic relatives are Staphylococcus intermedius and Staphylococcus delphini. Growth characteristics, biochemical features and DNA–DNA hybridizations demonstrated that the strains differ from these and other known species and that they represent a single, novel Staphylococcus species for which the name Staphylococcus pseudintermedius sp. nov. is proposed. The novel species is commonly confused with S. intermedius in routine diagnostic veterinary bacteriology. Although the strains described were isolated from lesions and show several characteristics typical of pathogenic staphylococci, such as coagulase, DNase and β-haemolysin production, the pathogenic significance of the novel species remains unclear. The type strain, LMG 22219T (=ON 86T=CCUG 49543T), was isolated from lung tissue of a cat.
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PETRIDOU (Ε. ΠΕΤΡΙΔΟΥ), E., R. FARMAKI (Ρ. ΦΑΡΜΑΚΗ), and A. F. KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ). "New aspects upon the classification of Staphylococcus intermedins." Journal of the Hellenic Veterinary Medical Society 61, no. 1 (November 13, 2017): 57. http://dx.doi.org/10.12681/jhvms.14877.
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In 1976, Staphylococcus intermedins was first described as being a new species isolated from pigeons, dogs, mink and horses. Until recently, the majority of coagulase-positive staphylococci originating from animals such as dogs and pigeons have been classified as S. intermedins. This species has been recognized to constitute normal skin flora of various animal species and to occasionally cause a variety of infections in dogs and cats. There have been six species of coagulase-positive staphylococci other than Staphylococcus aureus, namely S.intermedius, S.schleiferì subsp. coagulans, S.hyicus, S. lutrae, S.delphini and S. pseudintermedius. Sequence similarities of 16S rRNA genes among four of these species, except S. hyicus and S. lutrae, are 99% identical. In addition, it is very difficult to differentiate among S. intermedius, S. delphini and S. pseudintermedius phenotypically, and commercial kits are not available for the differentiation between of S. pseudintermedius and S. delphini. It has been shown that isolates phenotypically identified as Staphylococcus intermedius consist of four distinct species, including S. intermedius, Staphylococcus pseudintermedius and Staphylococcus delphini group A and B, which together represent the Staphylococcus intermedius group (SIG). Moreover, S. delphini group Β might be a novel species. In 2009, a new, rapid and inexpensive PCR-RFLP was developed and is capable of discriminating the SIG strains.
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Bierowiec, K., A. Korzeniowska-Kowal, A. Wzorek, K. Rypuła, and A. Gamian. "Prevalence of Staphylococcus Species Colonization in Healthy and Sick Cats." BioMed Research International 2019 (January 20, 2019): 1–10. http://dx.doi.org/10.1155/2019/4360525.
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Staphylococcus is one of the most frequently isolated genera of opportunistic bacteria in animals and human beings. Staphylococci in mammals mostly inhabit the skin and mucous membranes. The objectives of the study were to investigate the distribution of staphylococcal species in healthy and sick cats in order to find diagnostic markers. The risk factors associated with colonization were also explored. Isolates from healthy (n=520) and sick cats (n=67) were identified at the species level using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Swabs from conjunctival sacs, nares, skin, anus, and wounds were investigated using this technique. The diversity of the Staphylococcus species was high: 26 and 17 species in healthy and sick cats, respectively, and predominantly coagulase-negative staphylococci (CoNS) were isolated. The most frequently observed were S. felis and S. epidermidis in healthy cats, whereas S. felis and S. haemolyticus were most often found in sick animals. S. aureus strains were only isolated from healthy cats, whereas the only coagulase-positive Staphylococcus (CoPS) which occurred in the sick cats group was S. pseudintermedius. The sick, more frequently than the healthy animals, were colonized with S. pseudintermedius and S. haemolyticus and the relationship was statistically significant. Mostly, regardless of the state of their health, similar Staphylococcus species were isolated from cats; therefore, particular attention should be paid during the interpretation of diagnostic results.
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Andrade, Mariana, Ketlyn Oliveira, Catarina Morais, Patrícia Abrantes, Constança Pomba, Adriana E. Rosato, Isabel Couto, and Sofia Santos Costa. "Virulence Potential of Biofilm-Producing Staphylococcus pseudintermedius, Staphylococcus aureus and Staphylococcus coagulans Causing Skin Infections in Companion Animals." Antibiotics 11, no. 10 (September 30, 2022): 1339. http://dx.doi.org/10.3390/antibiotics11101339.
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Coagulase-positive staphylococci (CoPS) account for most bacteria-related pyoderma in companion animals. Emergence of methicillin-resistant strains of Staphylococcus pseudintermedius (MRSP), Staphylococcus aureus (MRSA) or Staphylococcus coagulans (MRSC), often with multidrug-resistant (MDR) phenotypes, is a public health concern. The study collection comprised 237 staphylococci (S. pseudintermedius (n = 155), S. aureus (n = 55) and S. coagulans (n = 27)) collected from companion animals, previously characterized regarding resistance patterns and clonal lineages. Biofilm production was detected for 51.0% (79/155), 94.6% (52/55) and 88.9% (24/27) of the S. pseudintermedius, S. aureus and S. coagulans, respectively, and was a frequent trait of the predominant S. pseudintermedius and S. aureus clonal lineages. The production of biofilm varied with NaCl supplementation of the growth media. All S. pseudintermedius and S. aureus strains carried icaADB. Kaplan–Meier survival analysis of Galleria mellonella infected with different CoPS revealed a higher virulence potential of S. aureus when compared with other CoPS. Our study highlights a high frequency of biofilm production by prevalent antimicrobial-resistant clonal lineages of CoPS associated with animal pyoderma, potentially related with a higher virulence potential and persistent or recurrent infections.
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Older, Caitlin E., Aline Rodrigues Hoffmann, Kathleen Hoover, and Frane Banovic. "Characterization of Cutaneous Bacterial Microbiota from Superficial Pyoderma Forms in Atopic Dogs." Pathogens 9, no. 8 (August 6, 2020): 638. http://dx.doi.org/10.3390/pathogens9080638.
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Although Staphylococcus pseudintermedius is considered the major pathogen associated with superficial canine pyoderma, no study has investigated the entire bacterial community in these lesions with molecular techniques. The objectives of this study were to characterize the bacterial microbiota in two forms of superficial canine pyoderma lesions, superficial bacterial folliculitis (SBF) and epidermal collarette (EC), especially in terms of the staphylococcal community. Swabs from 12 SBF and 9 EC lesions were obtained from eight and six atopic dogs, respectively. Eight samples from the axilla and groin of four healthy dogs served as controls. DNA was extracted for 16S rRNA gene sequencing and quantitative polymerase chain reaction of Staphylococcus spp. and S. pseudintermedius. Healthy skin samples harbored significantly more diverse bacterial communities than pyoderma samples. Healthy samples had communities that were more similar to each other, and were distinct from pyoderma samples. Staphylococcus spp. abundance was increased in pyoderma samples, especially those from EC samples. Although determining species-level identities of staphylococcal sequences revealed many species, S. pseudintermedius was the primary staphylococcal species found in all sample types. As expected, there are many differences in the microbiota when comparing healthy and canine pyoderma lesions samples. These lesions do not seem to be associated with a change in the relative abundance of specific Staphylococcus species, but simply an overall increase in Staphylococcus spp. abundance. The results of this study provide a starting point for future studies investigating how antimicrobial treatments may further change the microbiota associated with these lesions.
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Jarosiewicz, Małgorzata, Katarzyna Garbacz, Damian Neubauer, and Wojciech Kamysz. "In Vitro Efficiency of Antimicrobial Peptides against Staphylococcal Pathogens Associated with Canine Pyoderma." Animals 10, no. 3 (March 11, 2020): 470. http://dx.doi.org/10.3390/ani10030470.
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The emergence of staphylococcal canine pathogens resistant to multiple antimicrobial agents is a growing and urgent problem in veterinary practice. Antimicrobial peptides (AMPs) seem to be a promising alternative to conventional antibiotics. The aim of this in vitro study was to evaluate the antimicrobial activity of selected AMPs against pathogenic staphylococcal strains, including multidrug- and methicillin-resistant strains isolated from canine pyoderma cases. Seven antimicrobial peptides (aurein 1.2, CAMEL, citropin 1.1, protegrin-1, pexiganan, temporin A and uperin 3.6) synthesized by the 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase method were tested. The minimal inhibitory and minimal bactericidal concentrations (MIC and MBC) were determined by the broth microdilution method. The study showed that analyzed AMPs exerted an extensive effect against canine pathogens, with the most active peptide being uperin 3.6. The tested AMPs were equally efficient against both resistant- and susceptible staphylococcal strains and were more efficient against Staphylococcus pseudintermedius than against Staphylococcus aureus strains. Our findings are particularly interesting from a clinical perspective, as they point to AMPs as potential therapeutic topical agents in canine pyoderma cases associated with antimicrobial resistance of staphylococci.
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Elmoslemany, Ahmed, Ibrahim Elsohaby, Mohammed Alorabi, Mohamed Alkafafy, Theeb Al-Marri, Ali Aldoweriej, Fanan A. Alaql, Abdullah Almubarak, and Mahmoud Fayez. "Diversity and Risk Factors Associated with Multidrug and Methicillin-Resistant Staphylococci Isolated from Cats Admitted to a Veterinary Clinic in Eastern Province, Saudi Arabia." Antibiotics 10, no. 4 (March 31, 2021): 367. http://dx.doi.org/10.3390/antibiotics10040367.
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Understanding the distribution, antimicrobial resistance (AMR), and risk factors associated with multidrug-resistant (MDR) and methicillin-resistant staphylococci (MRS) isolated from cats admitted to veterinary clinics may decrease the risk of MDR and MRS transmission to humans and other cats. As such, the objectives of this study were to investigate the diversity in Staphylococcus spp. recovered from different anatomical locations in healthy and diseased cats and to determine the occurrence of MDR and MRS spp. as well as possible risk factors associated with colonization in these cats. Five swabs were collected from the anus, skin, ear canal, conjunctival sac, and nares of each cat (209 healthy and 191 diseased) admitted to a veterinary clinic in Eastern Province, Saudi Arabia, between January and December 2018. Prior to sample collection, cat owners completed a questionnaire collecting information on cat demographics, health status, management, and antimicrobial usage. In total, 179 Staphylococcus isolates were recovered from healthy (n = 71) and diseased (n = 108) cats, including 94 (52.5%) coagulase-positive staphylococci (CoPS), and 85 (47.5%) coagulase-negative staphylococci (CoNS). Five Staphylococcus spp. were identified, namely, Staphylococcus aureus, Staphylococcus pseudintermedius, Staphylococcus felis, Staphylococcus capitis, and Staphylococcus saprophyticus. Staphylococcus isolates were most commonly resistant to penicillin (56.4%) and ciprofloxacin (25.7%); however, no isolate was resistant to clindamycin. Thirty (16.8%) Staphylococcus spp. (24 S. aureus and 6 S. pseudintermedius) isolates were MDR, with resistance to up to six different antibiotic classes. Only 17 (9.5%) Staphylococcus spp. (15 methicillin-resistant S. aureus and 2 methicillin-resistant S. pseudintermedius) harbored the mecA gene. Risk factor analysis showed that cats with a history of antibiotic therapy, those raised mainly indoors with a child, and those who visit a veterinary clinic for treatment were at higher risk of MDR and MRS colonization. In conclusion, MDR and MRS were common in healthy and diseased cats in Saudi Arabia. Thus, an effective antimicrobial stewardship program and further studies using a One Health approach are required to investigate the role of cats as vectors for AMR transmission to humans.
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Bemis, David A., Rebekah D. Jones, Ricardo Videla, and Stephen A. Kania. "Evaluation of cefoxitin disk diffusion breakpoint for detection of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs." Journal of Veterinary Diagnostic Investigation 24, no. 5 (July 11, 2012): 964–67. http://dx.doi.org/10.1177/1040638712452112.
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Cefoxitin disk diffusion susceptibility testing is a recommended screening method for the detection of methicillin resistance in human isolates of Staphylococcus aureus and coagulase-negative staphylococci. A retrospective analysis of 1,146 clinical isolates of Staphylococcus pseudintermedius from dogs was conducted to determine if screening by the cefoxitin disk method can be similarly useful with S. pseudintermedius. The distribution of cefoxitin growth inhibition zone diameters within this collection was bimodal and correlated well with the results of methicillin resistance gene ( mecA) detection by polymerase chain reaction. Of the isolates, 5% had discordant results and, when retested, 84% of these were in agreement. While a greater diversity of isolates and interlaboratory comparisons must be tested, the current study suggests that an epidemiological breakpoint (of approximately ≤30 mm = resistant; ≥31 = susceptible) can be established to predict methicillin resistance in S. pseudintermedius.
Dissertations / Theses on the topic "Staphylococcus pseudintermediu"
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MERONI, GABRIELE. "MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCUS PSEUDINTERMEDIUS: RESEARCH ON VIRULENCE FACTORS, ANTIBIOTIC-RESISTANCE AND QUORUM-SENSING." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/704086.
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Staphylococcus pseudintermedius (S. pseudintermedius) is one of the most important pathogens in small animal veterinary medicine. In the dog, it is a commensal bacterium able to cause severe diseases (mainly dermatological), which rapidly evolve to persistent and chronic infections. This pathogen is also considered a public health problem due to the rapid evolution and spread of antibiotic-resistant clones, in particular, the worldwide dissemination of Multi-Drug Resistant (MDR) strains and methicillin-resistant S. pseudintermedius (MRSP). Nowadays, its zoonotic potential is under constant investigations. The first chapter of the thesis centers the attention on the typing techniques used to study a population of S. pseudintermedius isolated from the skin of dogs and raw milk of Holstein-Fresian dairy cattle. The aims of this study were the investigation of the genetic relatedness of different S. pseudintermedius strains using three molecular typing techniques; the Ribosomal Spacers Amplification PCR (RSA-PCR), the Random Amplification of Polymorphic DNA PCR (RAPD-PCR) and the Restriction Fragment Length Polymorphism PCR (RAPD-PCR). Moreover, the dissemination of species-specific genes coding for exfoliative toxins (siet, expA, and expB), enterotoxin (seCcanine) and a bicomponent leukocidin (lukS-F) was studied to understand the virulence of the strains. The study was carried out on a final population of 40 S. pseudintermedius strains isolated from 42 clinical cases of deep canine pyoderma and from 5 samples of raw milk from Holstein-Fresian dairy cattle with intramammary infection. After the microbiological examination and phenotypic typing, 47 S. pseudintermedius strains were collected and genetically analyzed. RSA-PCR revealed the presence of a unique biotype. The dendrogram obtained from RAPD-PCR disclosed two important results: i) the staphylococcal strains isolated from dogs and cows are genetically similar underlying the ability of this pathogen to colonize also dairy cattle; ii) the presence of a single cluster grouping all the S. pseudintermedius strains. Based on the presence of a specific restriction site in the phosphotransacetylase (pta) gene, RFLP-PCR confirmed the presence of 40 S. pseudintermedius and 7 non-S. pseudintermedius strains. The sequencing unveiled the effective presence of one strain of S. aureus and one of E. faecalis, 5 strains of S. schleiferi and 40 of S. pseudintermedius. All the S. pseudintermedius isolates were positive for siet gene, while expA and expB were amplified by, respectively, 10% and 17,5%. Specific enterotoxin seCcanine and leukotoxin luk-S-F were found with a prevalence of 35% and 92,5%, respectively. Therefore, the combined use of molecular typing techniques and virulence factors screening allows to better characterize S. pseudintermedius and to understand its zoonotic potential. The second chapter points out the attention to the problem of antibiotic resistance. Frequently, in Veterinary and Human medicine too, wide-spectrum antibiotics are prescribed to face with bacteria-based diseases without the microbiological examination and relative antibiotic-resistance screening. This could lead to the selection and spread of antibiotic-resistant strains. The aim of this work was to study the antibiotic-resistance profile of a large population of S. pseudintermedius strains collected in two Italian veterinary teaching hospitals, the first in Milan (Università degli Studi di Milano) and the second in Naples (Università degli Studi di Napoli “Federico II”) One-hundred sixteen and one-hundred twenty-six strains were collected in Milan and Naples, respectively. A panel of 10 different antibiotic molecules was tested with the Kirby-Bauer assay, qualitative PCR was used to describe the dissemination of mecA and tetM/K genes. The results showed a high rate of resistance for amoxicillin+clavulanate, clindamycin, tobramycin and tetracycline also confirmed by statistical significance. The prevalence of methicillin-resistant strains (MRSP) was 30% in Milan and18% in Naples also confirmed by the amplification of mecA gene. The cohort of methicillin-sensible strains in Naples (N= 103) was significantly more resistant compared to that from Milan (N= 81). In relation to the MDR population, all the MRSP in Milan and 91% in Naples are considered resistant to three or more pharmaceutical categories. These results totally agree with the recent report about the “Surveillance of antimicrobial resistance in Europe, 2017” and could be a starting point to set out a clearer use and prescription of antibiotics. The third chapter describes quorum-sensing and biofilm. It is known that bacteria are able to communicate via small peptides (Gram-positive) or lipids (Gram-negative) using a system called quorum-sensing. This complex behavior acts in response to external stimuli (e.g. cell density) and controls gene expression. Biofilm is one of the products of this communication, defined as a complex ecosystem of bacteria (sometimes also fungi and algae) enclosed in a self-produced matrix and adherent to a biotic or abiotic surface. This structure is frequently resistant to commonly used antibiotics and causes severe and more complex re-infections. The aim of the work was to describe the biofilm-forming ability of MDR and non-MDR S. pseudintermedius strains investigating the dissemination of virulence factors and antibiotic-resistance genes. A panel of 22 antibiotics was used to screen the antibiotic-resistance profiles of 73 S. pseudintermedius strains isolated from deep canine pyoderma. The biofilm-forming ability was investigated using a microtiter assay. The results clearly highlight a correlation between antibiotic-resistance and the ability to produce biofilm. All the MDR strains (N=42) are biofilm producers while 27 out of 31 non-MDR strains were able to produce biofilm. The last chapter of the thesis focused on an alternative strategy for the treatment of bacterial infections caused by Gram-positive and Gram-negative bacteria. For centuries, silver was used for its antimicrobial properties. Recently, the nanobiotechnology research area aimed to synthesize small particles (less than 100 nm) of silver metal to use as an alternative to traditional antibiotic-based therapies. The aim of this work was to synthesize silver nanoparticles using i) a Curcuma longa infusion and ii) the supernatant of E. coli. Furthermore, the antibacterial properties of synthesized nanoparticles were determined against 10 S. pseudintermedius strains and 10 Pseudomonas aeruginosa isolates by Kirby-Bauer disk diffusion assay and via Minimum Inhibitory Concentration (MIC). The anti-biofilm capacities were also investigated using an in vitro model of biofilm growth. Results showed that silver nanoparticles exert strong antibacterial activity compared to antibiotic alone and these properties are magnified when antibiotic was adjuvated with silver. Nanoparticles are also able to disrupt mature biofilm by directly linking to the cell wall of bacteria causing bacterial lysis. The use of silver nanoparticles obtained with green technology (e.g plant or organism derived molecules) is an eco-friendly alternative to classical antibiotic-based therapies and can be used to fight against both Gram-positive and Gram-negative infections.
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Raupelytė, Eglė. "Koaguliazei teigiamų stafilokokų išskyrimas iš gyvūnų augintinių." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_133815-68093.
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Darbo tikslas: nustatyti koaguliazei teigiamų stafilokokų paplitimą tarp gyvūnų augintinių.
Darbo uždaviniai:
1. išskirti koaguliazei teigiamus stafilokokus iš gyvūnų augintinių nosies ertmės;
2. išskirti koaguliazei teigiamus stafilokokus iš gyvūnų augintinių tiesiosios žarnos;
3. identifikuoti išskirtas stafilokokų padermes;
4. įvertinti įvairių veiksnių įtaką stafilokokų paplitimui;
5. nustatyti išskirtų stafilokokų atsparumą antimikrobinėms medžiagoms.
Darbo apimtis – 50 puslapių. Šiame darbe yra 6 lentelės bei 14 paveikslų. Magistro darbą sudaro 4 dalys. Pirmojoje dalyje apžvelgiami literatūros šaltiniai susiję su analizuojama tema, išskiriant koaguliazei teigiamų stafilokokų virulentiškumo veiksnius, atsparumą antimikrobinėms medžiagoms, sukeliamas ligas ir šių ligų gydymą. Aptariamas Staphylococcus aureus bei Staphylococcus pseudintermedius paplitimas ir paplitimą įtakojantys veiksniai. Antrojoje dalyje nurodyti tyrimo metodai, kuriais remiantis gauti duomenys tyrimų analizei. Trečiojoje dalyje analizuojami gauti tyrimo rezultatai pagal iškeltus uždavinius. Rezultatai pateikiami atsižvelgiant į statistinių duomenų patikimumą. Ketvirtoji dalis skirta literatūros apžvalgos ir tyrimo rezultatų skirtumų ir panašumų palyginimui.
Tyrimo metu iš gyvūnų augintinių nosies ertmės ir tiesiosios žarnos išskirti Staphylococcus aureus bei Staphylococcus pseudintermedius. Nustatyta, kad koaguliazei teigiamų stafilokokų paplitimas gyvūnų augintinių tarpe priklauso nuo gyvūnų... [toliau žr. visą tekstą]The The goal of the study: to determine prevalence of coagulase positive staphylococci in companion animals. The aim of the study: 1. to isolate coagulase positive staphylococci in nasal cavity of companion animals; 2. to isolate coagulase positive staphylococci in rectum of companion animals; 3. to identificate the isolated strains of staphylococci; 4. to evaluate risk factors for prevalence of staphylococci; 5. to determine antibiotic resistance in isolated staphylococci. The master study consists of 50 pages. It includes 6 tables and 14 pictures. The master study consist of 4 major chapters. The first chapter is dedicated to review of literature that is related with analized topic. This part includes coagulase positive staphylococci virulence factors, antibiotic resistance, diseases caused by staphylococci and treatment use. Furthermore chapter contains review of the prevalence and risk factors influenced the prevalence of Staphylococcus aureus and Staphylococcus pseudintermedius. The second chapter introduce with materials and methods, that were used in the research at this master study. In the third chapter the results of the research are presented. The results are presented according to the statistical reliability. The fourth chapter is the resemblance and similarity comparision of the literature review and master study research. In this master study Staphylococcus aureus and Staphylococcus pseudintermedius were isolated from nasal cavity and rectum of companion... [to full text]
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Couto, Natacha. "Insights into the dynamics of methicillin-resistant staphylococci in animals : a focus on Staphylococcus pseudintermedius in dogs." Doctoral thesis, Universidade de Lisboa. Faculdade de Medicina Veterinária, 2016. http://hdl.handle.net/10400.5/10771.
Full textAbstract:
Tese especialmente elaborada para a obtenção do grau de Doutor em Ciências Veterinárias, especialidade de ClínicaStaphylococci are a group of bacteria with clinical, agricultural, and economic importance because of their wide range of virulence factors and ability to become resistant to antimicrobials. This thesis has pursued three main objectives: I. Determine the frequency of methicillin-resistant S. aureus (MRSA) strains in several animal species, identify the characteristics of strains present in animals and comparison with human strains MRSA nasal screening was performed in 71 horses and 307 calves, and the observed frequencies were 3% and 2%, respectively. Seventy-four MRSA isolated from 2001 to 2014 were characterized: fourteen spa types, three SCCmec types and three clonal complexes (CC) 5, CC22 and CC398, were found. Most isolates were multidrug-resistant. Fourteen MRSA CC398 strains had qac genes (13 qacG and 1 qacJ), while 4 isolates (three CC5 and one CC22) had insertions in the norA promoter gene. MRSA linages from pets (CC5 and CC22) harboured specific sets of virulence genes and a lower number of resistance genes than CC398 from livestock-animals. II. Reveal antimicrobial/biocide susceptibility patterns/trends and resistance genes in methicillin-resistant staphylococci (MRS) Several antimicrobial resistance patterns and genes were found in MRS from horses. Minimum bactericidal concentrations of biocides chlorhexidine acetate, benzalkonium chloride, triclosan and glutaraldehyde were lower than the recommended in-use concentrations for veterinary medicine, although two MRS carried plasmid-borne qacA and sh-fabI or qacB and qacH-like genes. An investigation on the evolution of resistance to 38 antimicrobials, corresponding mechanisms and molecular characteristics of 644 clinical Staphylococcus spp. isolates obtained from companion animals between 1999-2014 revealed resistance to the majority of antimicrobials and the number of mecA-positive strains increased significantly over time. Considering S. pseudintermedius, the methicillin-susceptible (MSSP) were genetically more diverse than methicillin-resistant (MRSP). All MRSP and two MSSP strains were multidrug- resistant, with several antimicrobial resistance genes identified. One MSSP isolate harbored a qacA and another a qacB gene. Three biocide products had high bactericidal activity (Otodine®, Clorexyderm Spot Gel®, Dermocanis Piocure-M®), while Skingel® failed to achieve a five log reduction in the bacterial counting. III. Study of the pathogenesis of S. pseudintermedius in dogs The agr type III predominated in MRSP. Five virulence genes were found in all strains and only spsO gene was significantly associated with MSSP. MSSP produced more biofilm on BHIB and BHIB+1% glucose than MRSP isolates. Several virulence genes encoding surface proteins and toxins were highly expressed in the MRSP strain (compared to MSSP). By whole proteome characterization of S. pseudintermedius through 2DE MALDI-TOF/TOF MS approach we were able to identify 367 unique proteins, of which 39 were surface proteins. By subsequent use of the serological proteome analysis (SERPA) approach we identified 4 antigenic proteins with promising features for vaccine development. These results indicate that MRS were widely disseminated in the studied animal population, the environment and people in contact with these animals. The resistant trends and mechanisms detected in MRS strains are worrying and make animals a reservoir of important MRS clones and genes. Biocides are still a good therapeutic choice, even in the presence of efflux genes. Higher expression of virulence genes may play a role in the rapid and widespread of MRSP clones. Dogs are able to mount an IgG-response against S. pseudintermedius and the proteins identified by the immune system can in the future be used as vaccine candidates.
RESUMO - Estudo da dinâmica de estafilococos meticilina-resistente em animais – um foco no Staphylococcus pseudintermedius em cães - Os estafilococos são um grupo de bactérias com importância clínica, agrícola e económica devido à ampla gama de fatores de virulência e pela sua capacidade de se tornarem resistentes aos antimicrobianos. Esta tese debruçou-se sobre três objetivos principais: I. Determinar a frequência de estirpes S. aureus meticilina-resistente (MRSA) em diversas espécies animais, identificar as características das estirpes presentes em animais e comparar com estirpes humanas Colhemos zaragatoas de 71 cavalos e 307 vitelos para pesquisa de MRSA, e observaramse frequências de 3% e 2%, respetivamente. Foram caracterizadas setenta e quatro estirpes MRSA isoladas entre 2001-2014: catorze tipos de spa, três tipos de SCCmec e três complexos clonais (CC) 5, CC22 e CC398, foram encontrados. A maioria das estirpes (74%) eram multirresistentes. Catorze estirpes de MRSA CC398 tinha genes qac (13 qacG e 1 qacJ), enquanto 4 (três CC5 e um CC22) tinham inserções no gene promotor norA. As linhagens de MRSA de animais de estimação (CC5 e CC22) tinham conjuntos específicos de genes de virulência e um menor número de genes de resistência do que as linhagens associadas aos animais de produção (CC398). II. Revelar padrões/ tendências de suscetibilidade antimicrobiana/biocida e genes de resistência em estafilococos meticilina-resistente (MRS) Foram encontrados vários padrões e genes de resistência antimicrobiana em MRS de cavalos. As concentrações bactericidas mínimas dos biocidas acetato de clorhexidina, cloreto de benzalcónio, triclosan e glutaraldeído foram menores do que as recomendadas em medicina veterinária, embora dois MRS tivessem os genes plasmídicos qacA e sh-fabI ou qacB e um qacH-semelhante. Uma investigação sobre a evolução da resistência a 38 antimicrobianos, mecanismos correspondentes e características moleculares de 644 Staphylococcus spp. clínicos obtidos de animais de companhia entre 1999-2014 revelou resistência à maioria dos antimicrobianos. O número de estirpes mecA-positivo aumentou significativamente ao longo do tempo. Quanto aos S. pseudintermedius, os meticilina-suscetível (MSSP) eram geneticamente mais diversos do que os meticilina-resistente (MRSP). Todos os MRSP e 2 MSSP eram multirresistentes, com vários genes de resistência identificados. Um MSSP tinha um gene qacA e outro um qacB. Três produtos biocidas tinham elevada atividade bactericida (Otodine®, Clorexyderm Spot Gel®, Dermocanis Piocure-M®), enquanto Skingel® não conseguiu atingir uma redução de 5 log na contagem bacteriana. III. Estudo da patogenicidade de S. pseudintermedius em cães O tipo III agr predominou nos MRSP. Cinco genes de virulência foram encontrados em todas as estirpes e só o gene spsO foi significativamente associado com MSSP. MSSP produziu mais biofilme em BHIB e BHIB + 1% glucose que as estirpes de MRSP. Vários genes de virulência que codificam proteínas e toxinas de superfície foram altamente expressos na estirpe MRSP (em comparação com MSSP). Através da caracterização do proteoma total de S. pseudintermedius pela abordagem 2DE MALDI-TOF/TOF MS fomos capazes de identificar 367 proteínas únicas, das quais 39 eram proteínas de superfície. Posteriormente utilizámos a análise do proteoma serológico (SERPA) que identificou quatro proteínas antigénicas com características promissoras para o desenvolvimento de vacinas. Estes resultados indicam que MRS estavam amplamente disseminados na população animal estudada, no ambiente e nas pessoas em contato com esses animais. As tendências de resistência e os mecanismos detetados em estirpes MRS são preocupantes tornando os animais um reservatório de clones MRS e genes. Os biocidas ainda são uma boa opção terapêutica, mesmo na presença de bombas de efluxo. Uma maior expressão de genes de virulência pode desempenhar um papel na rápida expansão de clones de MRSP. Os cães foram capazes de montar uma resposta IgG contra S. pseudintermedius e as proteínas identificadas pelo sistema imunológico podem, no futuro, ser utilizadas como candidatos vacinais.
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Richards, Amy Claire. "Functional characterisation of Staphylococcus pseudintermedius cell wall-associated proteins." Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/25715.
Full textAbstract:
Staphylococcus pseudintermedius is the major cause of the common canine skin disease, pyoderma, and is a zoonotic pathogen of humans. Multidrug resistant strains of S. pseudintermedius have emerged and are spreading globally leading to decreased therapeutic success. The development of novel therapeutics is hindered by the lack of understanding of critical host-pathogen interactions mediating S. pseudintermedius colonization and pathogenesis. For the major human pathogen Staphylococcus aureus, interactions with host fibrinogen play a fundamental role in pathogenesis. The aim of the current study was to genetically and functionally characterise 2 cell wall-associated proteins of S. pseudintermedius, SpsD and SpsL, which mediate binding to multiple host extracellular matrix proteins including fibrinogen and fibronectin. DNA sequencing of the A- (ligand binding) domains of spsD and spsL genes for 37 phylogenetically diverse isolates revealed a highly conserved sequence for SpsL (97.1% derived amino acid identity), in contrast to more extensive variation for SpsD (76.7% derived amino acid identity). Further, recombination events were identified to have contributed to the evolution of spsD diversity. Functional analysis with gene deletion mutants of S. pseudintermedius strain ED99, constructed in the current study, demonstrated that SpsL is a major cell wall-associated fibrinogen-binding protein with enhanced affinity for canine fibrinogen. Using recombinant chains of fibrinogen it was determined that SpsL binds to the α-chain of fibrinogen similar to clumping factor B (ClfB) of S. aureus. However, ELISA and surface plasmon resonance analyses of recombinant truncated derivatives of SpsL indicated that the predicted ligand-binding N2N3 subdomains of the A-domain of SpsL are not sufficient for high-affinity fibrinogen-binding suggesting that either additional domains or post-translational modifications are required for fibrinogen-binding. Furthermore, development of a murine skin infection model allowed an investigation of the contribution of SpsD and SpsL to pathogenesis revealing a role for SpsL in focal abscess pathology. Overall these studies have provided new insights into the diversity, function and therapeutic potential of S. pseudintermedius fibrinogen-binding proteins.
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Juodžentytė, Renalda. "Staphylococcus aureus ir Staphylococcus pseudintermedius išskyrimas iš gyvūnų augintinių ir jų savininkų." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_133603-55635.
Full textAbstract:
Šio darbo tikslas - Staphylococcus aureus ir Staphylococcus pseudintermedius išskyrimas iš gyvūnų augintinių, jų savininkų ir antimikrobinių medžiagų atsparumo nustatymas.
Darbo uždaviniai:
1. Išskirti stafilokokus iš gyvūnų augintinių.
2. Išskirti stafilokokus iš gyvūnų augintinių savininkų.
3. Identifikuoti koaguliazei teigiamus stafilokokus.
4. Įvertinti įvairių veiksnių įtaka šunų nosies ertmės ir tiesiosios žarnos mikroflorai.
5. Įvertinti įvairių veiksnių įtaka gyvūnų augintinių savininkų nosies ertmės mikroflorai.
6. Nustatyti atsparumą antimikrobinėms medžiagoms.
Iš viso paimti 123 mėginiai, iš jų 82 mėginiai paimti iš šunų laikomų namuose ir lauke, ir 41 mėginys iš jų savininkų. Mėginiai mikrobiologiniam tyrimui buvo imami į transportines terpes TRANSWAB® (naudojama pernešimo terpė aerobams ir anaerobams išskirti (Amies, Liofilchem, Italija)).
Mikroorganizmų išskirta iš 37 (30,1 proc.) mėginių. Po vieną mikroorganizmų rūšį nustatyta 33 (89,1 proc.) mėginiuose. Po dvi mikroorganizmų rūšis (mišri infekcija), nustatyta 4 (10,9 proc.) mėginiuose.
Iš šunų nosies ertmės ir tiesiosios žarnos paimto 41 mėginio mikroorganizmai buvo išskirti iš 27 (59,3 proc.). Staphylococcus pseudintermedius sudarė 32,4 proc. išskirtų mikroorganizmų, Staphylococcus aureus – 12,2 proc., Bacillus spp. – 14,7 proc.
Iš šunų savinikų nosies ertmės paimto 41 mėginio mikroorganizmai buvo išskirti iš 16 (55,9 proc.) mėginių. Staphylococcus aureus sudarė 36,5 proc. išskirtų mikroorganizmų... [toliau žr. visą tekstą]The objective of the research: Staphylococcus aureus and Staphylococcus pseudintermedius isolation from a pet and their owners, and determine the resistance of isolates to antimicrobial agent. Tasks of the research: 1. To isolate staphylococci from pet. 2. To isolate staphylococci from pet owners. 3. To identify coagulase-positive staphylococci. 4. Evaluate the influence of canine nasal and rectal flora. 5. Evaluate the influence of the pet owners nasal cavity flora. 6. To determine the resistance of isolates to antimicrobial agent. 41 samples of dogs and 41 from humans were investigated. Total take 123 samples, including 82 samples were taken from dogs kept in the home and outdoors, and 41 samples of their owners. Samples for microbiological analysis were collected in transport medium TRANSWAB ® (used to transport medium for aerobes and anaerobes release (Amies, Liofilchem, Italy)). Microorganisms were identified in 37 (30.1 percent.) samples. Single type of microorganisms were identified in 33 (89.1 percent) samples. Two types of microorganisms (mixed infection) were identified in 4 (10.9 percent) samples. In dogs, nasal and rectal were taken 41 samples. Microorganisms were identified in 27 (59.3 percent) samples. Staphylococcus pseudintermedius accounted 32.4 percent of identified microorganisms, Staphylococcus aureus – 12.2 percent, Bacillus spp. – 14.7 percent. Dogs owners nasal were taken 41 samples. Microorganisms were identified in 16 (55.9 percent) samples... [to full text]
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Alvarez, Vega Luis Guillermo. "Detección de Staphylococcus pseudintermedius y Staphylococcus aureus aislados de piodermias caninas mediante PCR-RFLP." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2019. https://hdl.handle.net/20.500.12672/11838.
Full textAbstract:
A nivel mundial la piodermia es una de las enfermedades de la piel más diagnosticada en
caninos. Entre los agentes involucrados se encontraba Staphylococcus intermedius, el que
se creía el principal agente de las piodermias caninas; sin embargo, en el año 2005 fue
reclasificado en 3 especies: S. intermedius, S. pseudintermedius y S. delphini.
Posteriormente, estudios en diferentes países reportaron que el Staphylococcus
pseudintermedius es en realidad el agente bacteriano más frecuentemente aislado en
piodermias, seguidamente surgieron los primeros reportes de aislados resistentes a
meticilina. Por otro lado, Staphylococcus aureus, patógeno importante en medicina
humana, se aísla con más frecuencia en muestras de piodermias caninas, siendo estos
potenciales reservorios para reinfecciones en humanos de cepas resistentes a antibióticos.
Por ello, este estudio buscó determinar la presencia S. pseudintermedius y S. aureus en
141 aislados de Staphylococcus sp. provenientes de casos de piodermia canina del periodo
2016 - 2018. El ADN de los 141 aislados fue extraído y analizado mediante PCR-RFLP,
el cual consistió en la amplificación del gen pta y la digestión con la enzima MboI.
Obteniendo que los aislados de Staphylococcus sp. fueron identificados como
Staphylococcus pseudintermedius en un 87.9%, 12.1% como otros Staphylococcus sp. y
no se detectó Staphylococcus aureus. Concluyéndose que el Staphylococcus más
frecuentemente involucrado en piodermias caninas es el Staphylococcus
pseudintermedius; sin embargo, no se debe omitir el rol potencial que puede cumplir
Staphylococcus aureus como patógeno en caninos.Universidad Nacional Mayor de San Marcos (Lima). Vicerrectorado de Investigación y Posgrado
Tesis
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Couto, Natacha. "Nasal carriage of methicillin-resistant coagulase-positive staphylococci among cats and dogs hospitalized in the Veterinary Teaching Hospital of the Faculty of Veterinary Medicine – Technical University of Lisbon, Portugal." Bachelor's thesis, Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária, 2010. http://hdl.handle.net/10400.5/2177.
Full textAbstract:
Dissertação de Mestrado Integrado em Medicina VeterináriaMethicillin-resistant coagulase-positive staphylococci (MRCPS) colonization in companion animals is an emerging and significant problem in public and animal health. During one year, nasal swabs were obtained from 40 cats and 146 dogs admitted to the Teaching Hospital of the Faculty of Veterinary Medicine - Technical University of Lisbon. MRCPS colonization was screened by plating enrichment cultures on a selective medium, Chrom MRSA ID. Bacterial species and mecA were confirmed by PCR. Clonality of the isolates was assessed by pulsed-field gel electrophoresis (PFGE). All isolates were subjected to spa and SCCmec typing. They were also tested by PCR for the lukF/lukS genes encoding Panton-Valentine leukocidin (PVL) in Staphylococcus aureus and Luk-I and Staphylococcus intermedius exfoliative toxin (SIET) in Staphylococcus pseudintermedius. Methicillin-resistant S. aureus (MRSA) was found in two cats (5 %) and one dog (0.6 %). Isolates were spa type t032, SCCmec IV and shared identical PFGE profiles. These were similar to the EMRSA-15 human clone. Strains were PVL-negative. Nine dogs carried methicillin-resistant S. pseudintermedius (MRSP) (6 %), whereas none of the cats was positive. The PFGE type A strain (n=1) showed identical characteristics as the American MRSP clone strains (CC68-MRSP-V), while PFGE cluster B grouped European MRSP isolates (CC71-MRSP-III) (n=8). All isolates were SIETnegative. The 8 European MRSP isolates were positive for the lukF/lukS genes and the American MRSP isolate was negative for both genes. Strains were multidrug-resistant, which represents a major challenge for veterinarians in terms of antibiotic therapy.
RESUMO - Frequência de colonização por staphylococci coagulase-positivo meticilinaresistente em cães e gatos internados no hospital escolar da Faculdade de Medicina Veterinária – Universidade Técnica de Lisboa, Portugal - A colonização por staphylococci coagulase-positivo meticilina-resistente (MRCPS) é um problema emergente e de grande importância em termos de saúde animal e pública. Durante um ano, zaragatoas nasais de 146 cães e 40 de gatos foram obtidas de animais internados no Hospital Escolar da Faculdade de Medicina Veterinária - Universidade Técnica de Lisboa. A colonização por MRCPSfoi pesquisada por inoculação de culturas de enriquecimento num meio selectivo, Chrom MRSA ID. As espécies de MRCPS e a amplificação do gene mecA por feita por PCR. A clonalidade dos isolados foi confirmada por PFGE. Todos os isolados foram sujeitos a tipagem spa e SCCmec. Os isolados de S. aureus meticilina-resistente (MRSA) e de S. pseudintermedius meticilina-resistente (MRSP) foram testados por PCR para a presença dos genes lukF/lukS que codificam, respectivamente, a leucocidina Panton-Valentine (PVL) e a leucocidina-I (Luk-I). Os isolados de MRSP foram ainda testados para a presença da toxina exfoliativa do S. intermedius (SIET). Nesta amostra, 0,6 % (n=1) dos cães testados e 5 % (n=2) dos gatos apresentaram MRSA. Os isolados de MRSA eram spa tipo t032, SCCmec IV e partilhavam padrões idênticos de PFGE. As estirpes eram idênticas ao clone humano EMRSA-15. Os 3 isolados eram PVL negativos. Nove cães apresentaram MRSP (6 %), enquanto nenhum dos gatos foi positivo. PFGE tipo A mostrou características idênticas ao clone americano de MRSP (CC68-MRSP-V) e PFGE tipo B agrupou os isolados europeus de MRSP (CC71-MRSP-III) (n=8). Os isolados de MRSP PFGE tipo B eram Luk-I positivos mas SIET negativos. Todas as estirpes de MRSP eram multirresistentes a várias classes de antibióticos, o que representa um desafio para os médicos veterinários em termos de estratégias de antibioterapia.
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Breteau, Muriel. "Study of Staphylococcus pseudintermedius phages : towards the development of phage therapy." Thesis, University of Warwick, 2016. http://wrap.warwick.ac.uk/86043/.
Full textAbstract:
The extensive use of antibiotics has led to the emergence of methicillin-resistant Staphylococcus pseudintermedius, a bacterium causing difficult-to-treat canine skin infection (pyoderma). The administration of bacteriophages (phage therapy) can be an alternative to antibiotic therapy. Lytic phages, which lyse their host, are considered the only appropriate type of phages for phage therapy as opposed to temperate phages, which can survive within their host (lysogeny). However, it is possible to mutate temperate phages so that they cannot establish lysogeny anymore. Phage λ virulent (Vir) mutants have lost the operator to which the CI repressor binds to inhibit the expression of lytic genes. As a result, Vir mutants are strictly lytic. The work presented in this thesis was undertaken to isolate S. pseudintermedius phages and gain knowledge about their biology with the aim to develop phage therapy to treat pyoderma. The work was novel; very few data were available on S. pseudintermedius phages and no data have been published on phage therapy to treat canine skin infection. Four temperate phage candidates were selected after phenotypic and genotypic characterisation. No lytic phages were found. Random mutagenesis approaches were unsuccessful for the isolation of Vir mutants. An operator and three point mutations leading to the absence of CI repressor binding to this operator were identified through gel shift assay. These mutations should lead to a virulent phenotype if introduced in the relevant phage genome through site-directed mutagenesis. A PCR-based assay was performed to explore how widespread lysogeny was in S. pseudintermedius: 11 out of 45 tested strains were positive for the presence of prophage genes. Bioinformatic analyses revealed some of the genetic characteristics of S. pseudintermedius phages: genomic circular permutation and the presence of a genetic switch similar to that of phage λ. The work reported in this thesis represents a first step towards understanding the biology of S. pseudintermedius phages and developing phage therapy.
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Bannoehr, Jeanette. "Molecular population and colonisation factor analysis of the Staphylococcus intermedius group." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/5716.
Full textAbstract:
The Gram-positive bacterium Staphylococcus intermedius is regarded as the major cause of canine pyoderma, a common skin infection of dogs. However, despite its clinical importance, the population genetic structure of S. intermedius is poorly understood. The current study examined the population genetic structure of S. intermedius using a multilocus DNA sequencing approach. A collection of 99 isolates phenotypically identified as S. intermedius and originating from a broad array of animal hosts in several different countries was investigated. Phylogenetic analysis indicated that the isolates belonged to three distinct species including S. intermedius, staphylococcus pseudintermedius, and Staphylococcus delphini, together referred to as the S. intermedius group (SIG). Importantly, it was discovered that all canine isolates investigated belonged to the S. pseudintermedius phylotype and it was concluded that S. pseudintermedius, not S. intermedius, is the common cause of canine pyoderma. Further, it was revealed that S. delphini is more clinically important than was previously thought. The allelic variation of agrD, which encodes the autoinducing peptide (AIP) of the agr quorum sensing system in staphylococci, was determined for all isolates. Four AIP variants were identified, including three which were present in all three species, suggesting that a common quorum sensing capacity has been conserved despite species differentiation in very different niches. Considerable clonal diversity was revealed within the S. pseudintermedius species, including several methicillin-resistant clones which have evolved by recent acquisition of the mecA gene. Using the sequence diversity identified, a simple diagnostic test was developed based on a PCR-RFLP approach to discriminate S. pseudintermedius from S. intermedius and S. delphini. Having established that S. pseudintermedius is the common canine pyoderma pathogen, this study aimed to investugate key host-pathogen interactions involved in colonisation of its canine host. Bioinformatic analysis of the whole genome sequence of a clinical isolate of S. pseudintermedius (strain ED99) revealed 17 genes encoding predicted LPXTG-containing cell wall-anchored (CWA) surface proteins. A diverse collection of S. pseudintermedius isolates and closely related staphylococcal species was screened for the presence of the genes encodng the novel CWA proteins. The majority of genes were widely distributed among the isolates examined, with nine genes being exclusive to S. pseudintermedius and eight being also present in other members of the SIG. In Gram-positive bacteria, a family of CWA proteins called microbial surgace components recognising adhesive matrix molecules (MSCRAMMs)mediates bacterial adherence to extracellular matrix proteins of the host. Three of the 17 predicted novel CWA proteins, designated SpsD, SpsL and SpsO, were selected for further characterisation of their role in host-pathogen interactions and were cloned and expressed on the surface of the surrogate host Lactococcus lactis. Solid phase adherence assays employing host extracellular matrix proteins and canine corneocytes were performed to identify host extracellular matrix proteins and canine corneocytes were performed to identify host receptors for the putative MSCRAMMs. L. lactis expressing SpsD demonstrated binding to fibronectin, fibrinogen and cytokeratin 10, SpsL mediated binding of L. lactis to fibronectin and canine fibrrinogen, and SpsD and SpsO both mediated L. lactis adherence to canine corneocytes. Additionally, a cell culture assay using a commercially available canine epidermal cell line was developed and the adherence of S. pseudintermedius ED99 and the L. lactis constructs to the cell line was tested. S. pseudintermedius ED99, but none of the MSCRAMM-expressing L. lactis strains, adhered to the canine epidermal cells in vitro, suggesting that receptors for S. pseudintermedius adherence which are present in ex vivo corneocytes are not present in undifferentiated canine epidermal cell line preparations. Take together, the present study provides broad new insights into the classification and evolution of the SIG, and the molecular interaction of S. pseudintermedius with its canine host.
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Mathews, Jennifer Leah. "Phenotypic and Genotypic Characterization of Methicillin-Resistant Staphylococcus aureus and Staphylococcus pseudintermedius at a Veterinary Teaching Hospital." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1339738135.
Full textBook chapters on the topic "Staphylococcus pseudintermediu"
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Murayama, Nobuo, Masahiko Nagata, Yuri Terada, Mio Okuaki, Noriyuki Takemura, Hidemasa Nakaminami, and Norihisa Noguchi. "In Vitro Antiseptic Susceptibilities for Staphylococcus Pseudintermedius Isolated from Canine Superficial Pyoderma in Japan." In Advances in Veterinary Dermatology, 137–40. Oxford, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118644317.ch17.
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Donovan, Zoe. "Resistance of canine Staphylococcus pseudintermedius to selected topical and systemic antimicrobials in the UK." In BSAVA Congress Proceedings 2020, 399. British Small Animal Veterinary Association, 2020. http://dx.doi.org/10.22233/9781910443774.51.3.
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Simjee, Shabbir, and Wesley Power. "Efficacy of florfenicol against Staphylococcus pseudintermedius recovered from dogs with otitis externa across the EU between 2017-2018." In BSAVA Congress Proceedings 2022. British Small Animal Veterinary Association, 2022. http://dx.doi.org/10.22233/9781913859114.38.10.
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Ollivier, Elodie, Claudine Zemirline, Laetitia Marchand, and Brigitte Closs. "Effect of the ingredient A97614A1 on the adhesion and biofilm formation of Staphylococcus pseudintermedius in a model of reconstructed canine epidermis." In BSAVA Congress Proceedings 2019, 442. British Small Animal Veterinary Association, 2019. http://dx.doi.org/10.22233/9781910443699.62.1.
Full textConference papers on the topic "Staphylococcus pseudintermediu"
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Garcês, Andreia, Augusto Silva, Ricardo Lopes, Filipe Sampaio, Daniela Duque, and Paula Brilhante-Simões. "Methicillin-Resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) in Skin Infections from Company Animals in Portugal (2013–2021)." In The 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action and Mechanisms of Resistance. Basel Switzerland: MDPI, 2022. http://dx.doi.org/10.3390/eca2022-12689.
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Matthew DiCicco, Suresh Neethirajan, Ameet Singh, and J Scott Weese. "In vitro Activity of Antimicrobials Alone and in Combination Therapy against Methicillin-resistant Staphylococcus pseudintermedius Biofilm Formation." In 2012 Dallas, Texas, July 29 - August 1, 2012. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2012. http://dx.doi.org/10.13031/2013.41886.
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Silva, Vanessa, Ana Oliveira, Manuela Caniça, Vera Manageiro, José Capelo, Gilberto Igrejas, and Patrícia Poeta. "Clonal diversity and antimicrobial resistance of <em>Staphylococcus pseudintermedius</em> isolated from canine pyoderma." In 1st International Electronic Conference on Microbiology. Basel, Switzerland: MDPI, 2020. http://dx.doi.org/10.3390/ecm2020-07136.
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Chaudhry, Y., A. Robinson, and K. S. Godinho. "Observations on the antimicrobial susceptibility of Staphylococcus pseudintermedius following the introduction of cefovecin for clinical use in Europe." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0064.
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Olivo Freites, C., D. Patadia, J. Saabiye, P. Kohli, C. Stavropoulos, and J. Salonia. "A Perfect Storm of Uncommon Pathogens in an Immunocompetent Host: Respiratory Isolation of Cunninghamella Berrhotheliae, Aspergillus Niger, Staphylococcus Pseudintermedius and Adenovirus in a Patient with Necrotizing Pneumonia." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a6836.
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Mesquita, Fernanda Fernandes de, Mergareth Balbi, Juliana Lopes De Castro, and Ramon Da Luz Bezerra. "PERFIL EPIDEMIOLOGICO DOS CÃES COM PIODERMA ATENDIDOS NA POLICLINICA VETERINÁRIA DA UNESA – VARGEM PEQUENA, NO PERÍODO DE OUTUBRO DE 2018 A ABRIL DE 2021." In I Congresso On-line Nacional de Clínica Veterinária de Pequenos Animais. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1816.
Full textAbstract:
Introdução: A piodermite é uma infecção bacteriana piogênica da pele, que envolve a epiderme e os folículos pilosos, podendo atingir derme e hipoderme nos quadros profundos. Quase sempre é secundária a uma causa base, sendo uma das dermatopatias mais comuns em cães. Classifica-se o pioderma em externo, superficial ou profundo. A principal bactéria envolvida é o Staphylococcus pseudintermedius. Acomete cães de qualquer idade, raça e sexo. O diagnóstico é clínico baseado nas lesões, sendo no pioderma superficial a pústula e o colarete epidérmico. A terapêutica baseia-se exclusivamente em antimicrobianos tópicos ou em uso conjunto com antibióticos sistêmicos. Objetivos: O presente trabalho levantou o perfil epidemiológico (idade, sexo e raça) dos cães com pioderma, atendidos na Policlínica Veterinária UNESA, no campus de Vargem Pequena, Rio de Janeiro, RJ. Além de classificar se o pioderma era externo, superficial ou profundo e se cursava com prurido. Material e Métodos: Levantou-se as fichas de cães que passaram por atendimento dermatológico da Policlínica entre outubro 2018 a abril 2021, destacando-se aquelas com diagnóstico de pioderma. Em seguida, coletou-se dados referentes a idades, sexos e raças. Na anamnese investigou-se prurido e no exame físico qual a classificação do pioderma e evolução aguda ou crônica. Resultados: Das 110 fichas de dermatopatias, 40 (36,3%) foram identificados com pioderma, sendo 21 (52,5%) fêmeas e 19 (47,5%) machos. Principais raças: 11 (27,5%) sem raça definida, 8 (20%) yorkshire e 5 (12,5%) Bulldog Francês. As idades variaram de 9 meses a 10 anos, predominando a idade 5 anos (8 cães, 20%). O prurido foi relatado em 33 (82%) casos. O pioderma superficial em 39 (97,5%) casos e 1 (2,5%) profundo. Já os casos crônicos (32 cães, 80%) agudos (8 cães, 20%). Conclusão: O pioderma superficial destacou-se nas dermatopatias, sem predisposição etária ou sexual, logo o conhecimento das lesões faz-se fundamental para seu diagnóstico. O prurido foi um sinal constante nesta dermatopatia e o seu controle está relacionado com o diagnóstico e terapêutica apropriada para o pioderma.