Academic literature on the topic 'Staphylococcus aureus biofilms'
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Journal articles on the topic "Staphylococcus aureus biofilms"
Wu, Julie A., Caroline Kusuma, James J. Mond, and John F. Kokai-Kun. "Lysostaphin Disrupts Staphylococcus aureus and Staphylococcus epidermidis Biofilms on Artificial Surfaces." Antimicrobial Agents and Chemotherapy 47, no. 11 (November 2003): 3407–14. http://dx.doi.org/10.1128/aac.47.11.3407-3414.2003.
Full textIzano, Era A., Matthew A. Amarante, William B. Kher, and Jeffrey B. Kaplan. "Differential Roles of Poly-N-Acetylglucosamine Surface Polysaccharide and Extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis Biofilms." Applied and Environmental Microbiology 74, no. 2 (November 26, 2007): 470–76. http://dx.doi.org/10.1128/aem.02073-07.
Full textSaising, Jongkon, Linda Dube, Anne-Kathrin Ziebandt, Supayang Piyawan Voravuthikunchai, Mulugeta Nega, and Friedrich Götz. "Activity of Gallidermin on Staphylococcus aureus and Staphylococcus epidermidis Biofilms." Antimicrobial Agents and Chemotherapy 56, no. 11 (August 27, 2012): 5804–10. http://dx.doi.org/10.1128/aac.01296-12.
Full textHoriuk, Y. V., M. D. Kukhtyn, Y. S. Stravskyy, S. I. Klymnyuk, K. M. Vergeles, and V. V. Horiuk. "Influence of staphylococcal Phage SAvB14 on biofilms, formed by Staphylococcus aureus variant bovis." Regulatory Mechanisms in Biosystems 10, no. 3 (August 22, 2019): 314–18. http://dx.doi.org/10.15421/021948.
Full textHrynchuk, N. I., N. O. Vrynchanu, T. A. Buchtyarova, D. M. Dudikova, Yu V. Korotkyi, and L. B. Bondarenko. "Antibiofilm Effect of Adamantane Derivative against Staphylococcus aureus." Mikrobiolohichnyi Zhurnal 83, no. 1 (February 17, 2021): 58–67. http://dx.doi.org/10.15407/microbiolj83.01.058.
Full textYarwood, Jeremy M., Douglas J. Bartels, Esther M. Volper, and E. Peter Greenberg. "Quorum Sensing in Staphylococcus aureus Biofilms." Journal of Bacteriology 186, no. 6 (March 15, 2004): 1838–50. http://dx.doi.org/10.1128/jb.186.6.1838-1850.2004.
Full textSinghal, Deepti, Andrew Foreman, Josh-Jervis Bardy, and Peter-John Wormald. "Staphylococcus aureus biofilms." Laryngoscope 121, no. 7 (June 6, 2011): 1578–83. http://dx.doi.org/10.1002/lary.21805.
Full textTuon, Felipe Francisco, Paula Hansen Suss, Joao Paulo Telles, Leticia Ramos Dantas, Nícolas Henrique Borges, and Victoria Stadler Tasca Ribeiro. "Antimicrobial Treatment of Staphylococcus aureus Biofilms." Antibiotics 12, no. 1 (January 4, 2023): 87. http://dx.doi.org/10.3390/antibiotics12010087.
Full textAchek, Rachid, Helmut Hotzel, Ibrahim Nabi, Souad Kechida, Djamila Mami, Nassima Didouh, Herbert Tomaso, et al. "Phenotypic and Molecular Detection of Biofilm Formation in Staphylococcus aureus Isolated from Different Sources in Algeria." Pathogens 9, no. 2 (February 24, 2020): 153. http://dx.doi.org/10.3390/pathogens9020153.
Full textSharma, Mrinalini, Livia Visai, Francesca Bragheri, Ilaria Cristiani, Pradeep Kumar Gupta, and Pietro Speziale. "Toluidine Blue-Mediated Photodynamic Effects on Staphylococcal Biofilms." Antimicrobial Agents and Chemotherapy 52, no. 1 (October 29, 2007): 299–305. http://dx.doi.org/10.1128/aac.00988-07.
Full textDissertations / Theses on the topic "Staphylococcus aureus biofilms"
Graziano, Talita Signoreti 1988. "Effects of statins in the bacterial viability and on biofilm of Staphylococcus aureus." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/289491.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2018-08-25T11:18:29Z (GMT). No. of bitstreams: 1 Graziano_TalitaSignoreti_M.pdf: 1603562 bytes, checksum: 9522f75e1f8c1147c84c0fde100549ed (MD5) Previous issue date: 2014
Resumo: As estatinas são um grupo de fármacos que atuam como inibidores competitivos da enzima 3-Hidroxi-3-MetilGlutaril Coenzima-A Redutase (HMG-CoA redutase). Além de atuarem como importantes agentes hipolipemiantes, também apresentam outros efeitos, chamados de pleiotrópicos. Diversos estudos têm explorado um possível efeito protetor das estatinas atuando na redução na morbidade e mortalidade de várias doenças infecciosas. A atividade antimicrobiana das estatinas tem sido reportada por estudos in vivo e in vitro. O objetivo desse estudo foi avaliar os efeitos das estatinas sobre o crescimento e viabilidade de bactérias aeróbias patogênicas, e o efeito da sinvastatina sobre o biofilme de Staphylococcus aureus. Culturas das espécies de Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli e Enterococcus faecalis foram avaliadas na forma planctônica quanto à sensibilidade à atorvastatina, pravastatina e sinvastatina, através do teste de Concentração Inibitória Mínima (CIM). Além disso, diante da atividade apresentada pela sinvastatina contra S. aureus, foi determinada a ação dessa droga sobre a viabilidade celular através dos testes de Time-kill e Efeito pós-antibiótico (EPA). Também foi verificado um possível efeito sinérgico entre a sinvastatina e vancomicina. Por fim, a ação da sinvastatina foi avaliada contra biofilmes de S. aureus. Os valores de CIM da sinvastatina para o microrganismo S. aureus foram: 15,65 µg/ml (ATCC 29213) e 31,25 µg/ml (ATCC 33591, 43300, 14458 e 6538). A sinvastatina apresentou um perfil bacteriostático, e na concentração de 4xCIM seu EPA foi similar ao da vancomicina. Não foi encontrado nenhum tipo de interação entre a associação de sinvastatina e vancomicina. Entretanto, a sinvastatina foi capaz de reduzir a formação do biofilme nas concentrações entre 1/8CIM à 4xCIM. Além disso, na concentração 4xMIC foi capaz de diminuir a viabilidade, biomassa e a produção de polissacarídeos extracelulares e aumentar a produção de polissacarídeos intracelulares de biofilmes maduros de S. aureus. A produção de proteínas pelo biofilme não foi alterada. Em conclusão, os resultados encontrados mostram que a sinvastatina possui um grande potencial a ser explorado, principalmente em relação ao descobrimento de novos antimicrobianos
Abstract: Statins are drugs that competitively inhibit the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA). Besides their important lipid-lowering action, they also are pleiotropic agents. Several studies have explored a possible protective effect of statins to reduce the morbidity and mortality of various infectious diseases. The antimicrobial activity of statins has been reported by in vivo and in vitro studies. The aim of this study was to evaluate the effects of statins on the growth, viability and biofilm formation of pathogenic aerobic bacteria. The Minimum Inhibitory Concentrations (MIC) of atorvastatin, pravastatin and simvastatin against planktonic cells of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis strains were obtained. Since simvastatin showed activity against S. aureus, its effects on cell viability were evaluated in a time-kill and post-antibiotic effect (PAE) assays. A possible synergistic effect between simvastatin and vancomycin was also assessed. In addition, the effect of simvastatin against biofilms of S. aureus was tested. The MIC values of simvastatin for S. aureus were: 15.65 µg/ml (ATCC 29213) and 31.25 µg/ml (ATCC 33591, 43300, 14458 and 6538). Simvastatin showed a bacteriostatic profile, and in a 4x>MIC concentration the PAE was similar to vancomycin. No synergistic effect was found between simvastatin and vancomycin. Simvastatin was able to reduce the formation of biofilms in concentrations ranging from 1/8MIC to 4xMIC. In addition, the 4xMIC was able to decrease the viability, biomass and production of extracellular polysaccharides and increase the production of intracellular polysaccharides on mature biofilm of S. aureus. The protein production on biofilm was not altered in the presence of simvastatin . In conclusion, our results showed that simvastatin has a great potential to be explored, especially in relation to the development new antimicrobial agents
Mestrado
Farmacologia, Anestesiologia e Terapeutica
Mestra em Odontologia
Marques, Claire. "Etude de l'impact d'antibiotiques sur des biofilms de Staphylococcus aureus." Thesis, Clermont-Ferrand 1, 2016. http://www.theses.fr/2016CLF1PP02.
Full textOsteoarticular infections (OAI) often require a surgical procedure with prosthesis removal followed by long-term complex antibiotherapy. The ability of Staphylococcus aureus to adhere and produce biofilm on the surface of implanted material contributes to treatment failures and microbiological relapses. In addition, biofilm formation can be induced by some antibiotics at sub-minimal inhibitory concentrations (sub-MICs). The present study characterizes in vitro the effects of 12 antibiotics on biofilm formed by a strain of methicillin-susceptible Staphylococcus aureus isolated from an osteo-articular infection.The influence of these antibiotics was assessed on biofilm formation at concentrations including the breakpoints, by numbering viable cells in the biofilm biomass and in the suspensions (unattached cells) surrounding the biofilm. Biofilm formation was prevented in presence of ceftarolin, daptomycin, fosfomycin, gentamicin, ofloxacin, rifampicin and vancomycin at the highest concentrations tested. Only fosfomycin showed inhibition properties also at sub-MICs. Unattached and sessile viable bacteria were undetectable to daptomycin and gentamicin at the highest concentrations tested.Determination of the minimum biofilm eradication concentrations (MBECs) indicated that in vitro eradication of 24h-old biofilms required concentrations at least 800 times higher than the planktonic MIC, concentrations obviously not compatibles with classical therapeutic doses.In the second part of this work, we focused our study on the action of fosfomycin, because of its effect on biofilm formation and its therapeutic interest. A transcriptome analysis was performed with sessile cells from both biofilm formed in the presence of sub-MIC of fosfomycin and cells from pre-formed 24h-old biofilm treated by fosfomycin at sub-MBEC. Fosfomycin induced mostly down regulation of genes assigned to nucleotide, amino acid and carbohydrate transport and metabolism. Adhesins and capsular biosynthesis proteins (ScdA) encoding genes were also down regulated. To a lesser extent, peptidoglycan biosynthesis proteins (MGT and MurA) and autolysins encoding genes were found down regulated. Metabolic slowdown and cell membrane modifications induced by fosfomycin are likely to be responsible for the impairment of bacterial adhesion capacity.The action of fosfomycin at sub-MIC on unattached cells surrounding biofilm was also analyzed. Surprisingly, they displayed higher capacity to form new biofilm than their counterparts obtained without fosfomycin, probably associated with their large peptidoglycan layer.In conclusion, these data underline the relevance of the use of fosfomycine in preventing osteo-articular infections due to S. aureus and should be assessed in in vivo experiments. However, simultaneous eradication of unattached cells should also be considered due to the high capacities of these cells to disseminate and establish new biofilm, a real risk of treatment failure
Pinto, Geraldo Camilo de Souza [UNESP]. "Eficácia da terapia fotodinâmica antimicrobiana em biofilmes de Staphylococcus Aureus suscetível e resistente á meticilina." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/97310.
Full textA necessidade de superar o desafio criado pelos biofilmes resistentes aos tratamentos antimicrobianos convencionais tem levado à busca por tratamentos alternativos, como terapia fotodinâmica antimicrobiana (aPDT). Este estudo avaliou in vitro a eficácia da aPDT na inativação de biofilmes de Staphylococcus aureus suscetíveis e resistentes à meticilina (MRSA e MSSA), mediado pelos fotossensibilizadores (PSs) Curcumina (Cur) e Photodithazine® (PDZ). Biofilmes foram formados e tratados com diferentes concentrações de Cur (0, 20, 40 e 80 μM) e PDZ (0, 50 e 75 mg/L), e iluminados ou não por fonte de luz LED (Cur 455 ± 3 nm/ 5,28 J/cm2; PDZ 660 ± 3 nm/ 5,28 J/cm2 ou 50 J/cm²). Os grupos Controle Positivo (CP) não receberam nenhum PS e também não foram iluminados. A viabilidade dos micro-organismos após a aPDT foi avaliado pelo número de colônias viáveis, pelo ensaio de XTT e pela utilização do kit LIVE/DEAD® na Microscopia Confocal de Varredura à Laser (MCVL). Os resultados foram avaliados por análises de variância de dois fatores de efeitos fixos (ANOVA) e complementados por comparações múltiplas de médias pelo teste de Tukey. Para ambas as cepas, todas as concentrações de Cur e PDZ testadas reduziram significativamente a atividade metabólica e o UFC/mL para ambos micro-organismos quando comparado com os grupos CN (p0,05). Os resultados foram otimizados para a Cur quando utilizou-se a maior concentração (80 μM), para a PDZ, a maior redução nos micro-organismos foi observada quando associou-se a maior concentração de PDZ (75 mg/L) com a maior dose de luz (50 J/cm²). Os biofilmes submetidos a aPDT demostraram pela MCVL um maior número de células coradas em vermelho, indicando que a aPDT foi eficaz para promover danos ou morte às células bacterianas. Assim, a aPDT pode ser considerada promissora para atuar de forma sinérgica no tratamento de infecções bacterianas
The need to overcome the challenge created by biofilms regarding conventional antimicrobial approaches has lead to search of alternative treatments such as Antimicrobial Photodynamic Therapy (aPDT). This in vitro study evaluated the efficacy of aPDT using the photosensitizer (PS) Curcumin (Cur) and Photodithazine® (PDZ) in the inactivation of biofilms of methicillin susceptible and resistant S. aureus (MSSA and MRSA). Biofilms were treated with different Cur (0, 20, 40 or 80 μM of Cur) and PDZ concentrations (0, 50 or 75 mg/L) and illuminated or not with LED source (Cur 455 ± 3 nm/ 5.28 J/cm2; PDZ 660 ± 3 nm/ 5.28 J/cm2 or 50 J/cm²). Positive control samples were not exposed to PS or light. The microorganisms viability after aPDT were evaluated by counting the number of colonies, the XTT assay and LIVE/DEAD® staining using confocal laser scanning microscopy (CLSM). The results were evaluated by analysis of variance, two-factor fixed effects (ANOVA) and complemented by multiple comparisons by Tukey test. For both strains, all the tested Cur and PDZ concentrations reduced significantly both biofilm metabolic activity and CFU/mL compared to the negative control (p0.05). Moreover, the results were optimized for Cur when the higher concentration was used (80 μM); For PDZ, the best results were obtained when it was associated a higher concentration of PDZ (75 mg/L) with the higher dose of light (50 J/cm²). Biofilms submitted to aPDT showed a large number of red-stained colonies, indicating that this therapy was efficient in disrupting the bacterial membrane. It can be concluded that PS was efficient in reducing viable colonies of both S. aureus strains by damaging cell membrane and causing cell death. Thus, the aPDT is can be considered promising to act synergistically in the treatment of bacterial infections
Pinto, Geraldo Camilo de Souza. "Eficácia da terapia fotodinâmica antimicrobiana em biofilmes de Staphylococcus Aureus suscetível e resistente á meticilina /." Araraquara, 2013. http://hdl.handle.net/11449/97310.
Full textBanca: Eunice Teresinha Giampaolo
Banca: Ana Paula Dias Ribeiro
Resumo: A necessidade de superar o desafio criado pelos biofilmes resistentes aos tratamentos antimicrobianos convencionais tem levado à busca por tratamentos alternativos, como terapia fotodinâmica antimicrobiana (aPDT). Este estudo avaliou in vitro a eficácia da aPDT na inativação de biofilmes de Staphylococcus aureus suscetíveis e resistentes à meticilina (MRSA e MSSA), mediado pelos fotossensibilizadores (PSs) Curcumina (Cur) e Photodithazine® (PDZ). Biofilmes foram formados e tratados com diferentes concentrações de Cur (0, 20, 40 e 80 μM) e PDZ (0, 50 e 75 mg/L), e iluminados ou não por fonte de luz LED (Cur 455 ± 3 nm/ 5,28 J/cm2; PDZ 660 ± 3 nm/ 5,28 J/cm2 ou 50 J/cm²). Os grupos Controle Positivo (CP) não receberam nenhum PS e também não foram iluminados. A viabilidade dos micro-organismos após a aPDT foi avaliado pelo número de colônias viáveis, pelo ensaio de XTT e pela utilização do kit LIVE/DEAD® na Microscopia Confocal de Varredura à Laser (MCVL). Os resultados foram avaliados por análises de variância de dois fatores de efeitos fixos (ANOVA) e complementados por comparações múltiplas de médias pelo teste de Tukey. Para ambas as cepas, todas as concentrações de Cur e PDZ testadas reduziram significativamente a atividade metabólica e o UFC/mL para ambos micro-organismos quando comparado com os grupos CN (p0,05). Os resultados foram otimizados para a Cur quando utilizou-se a maior concentração (80 μM), para a PDZ, a maior redução nos micro-organismos foi observada quando associou-se a maior concentração de PDZ (75 mg/L) com a maior dose de luz (50 J/cm²). Os biofilmes submetidos a aPDT demostraram pela MCVL um maior número de células coradas em vermelho, indicando que a aPDT foi eficaz para promover danos ou morte às células bacterianas. Assim, a aPDT pode ser considerada promissora para atuar de forma sinérgica no tratamento de infecções bacterianas
Abstract: The need to overcome the challenge created by biofilms regarding conventional antimicrobial approaches has lead to search of alternative treatments such as Antimicrobial Photodynamic Therapy (aPDT). This in vitro study evaluated the efficacy of aPDT using the photosensitizer (PS) Curcumin (Cur) and Photodithazine® (PDZ) in the inactivation of biofilms of methicillin susceptible and resistant S. aureus (MSSA and MRSA). Biofilms were treated with different Cur (0, 20, 40 or 80 μM of Cur) and PDZ concentrations (0, 50 or 75 mg/L) and illuminated or not with LED source (Cur 455 ± 3 nm/ 5.28 J/cm2; PDZ 660 ± 3 nm/ 5.28 J/cm2 or 50 J/cm²). Positive control samples were not exposed to PS or light. The microorganisms viability after aPDT were evaluated by counting the number of colonies, the XTT assay and LIVE/DEAD® staining using confocal laser scanning microscopy (CLSM). The results were evaluated by analysis of variance, two-factor fixed effects (ANOVA) and complemented by multiple comparisons by Tukey test. For both strains, all the tested Cur and PDZ concentrations reduced significantly both biofilm metabolic activity and CFU/mL compared to the negative control (p0.05). Moreover, the results were optimized for Cur when the higher concentration was used (80 μM); For PDZ, the best results were obtained when it was associated a higher concentration of PDZ (75 mg/L) with the higher dose of light (50 J/cm²). Biofilms submitted to aPDT showed a large number of red-stained colonies, indicating that this therapy was efficient in disrupting the bacterial membrane. It can be concluded that PS was efficient in reducing viable colonies of both S. aureus strains by damaging cell membrane and causing cell death. Thus, the aPDT is can be considered promising to act synergistically in the treatment of bacterial infections
Mestre
Blanchard, Alex Paul. "Peroxygen disinfection of Pseudomonas aeruginosa and Staphylococcus aureus biofilms." Thesis, University of Bath, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342075.
Full textChapman, Jenelle. "BIOFILMS: A DEVELOPMENTAL NICHE FOR VANCOMYCIN-INTERMEDIATE RESISTANT STAPHYLOCOCCUS AUREUS." OpenSIUC, 2017. https://opensiuc.lib.siu.edu/theses/2204.
Full textFernandes, Renan Aparecido. "Fitossíntese de nanopartículas de prata a partir de extrato de cascas de romã e desenvolvimento de formulação para tratamento de feridas : avaliação antimicrobiana, citotóxica e potencial cicatrizante em um modelo in vivo /." Araçatuba, 2017. http://hdl.handle.net/11449/151982.
Full textCoorientadora: Andresa Aparecida Berretta e Silva
Banca: Alberto Carlos Botazzo Delbem
Banca: Denise Pedrini Ostini
Banca: Luiz Fernando Gorup
Banca: Elaine Cristina Guerbach Conti
Resumo: O objetivo deste estudo foi avaliar a capacidade de produção de nanopartículas de prata através do extrato da casca de romã, e produzir formulações contendo estas nanopartículas para uso em feridas. Elas foram testadas quanto à ação antimicrobiana, citotóxica e potencial cicatrizante. Para a produção das nanopartículas de prata propôs-se uma síntese utilizando-se como base duas metodologias já estabelecidas na literatura, utilizando-se para reação carboximetilcelulose, propilenoglicol, nitrato de prata, água e extrato da casca de romã como agente redutor. O extrato da casca de romã foi caracterizado em parâmetros como pH, massa seca e quantidade de taninos bioativos (ácido elágico e totais fenólicos expressos em ácido gálico). Os totais fenólicos do extrato foram também dosados após seu aquecimento nas diferentes condições de tempo e temperatura propostos para as sínteses das nanopartículas de prata (12 minutos, 1 hora e 2 horas, à 50ºC e 100ºC). As nanopartículas de prata produzidas foram, então, adicionadas a uma solução contendo compostos para produção de formulações para serem utilizadas no tratamento de feridas. Elas foram caracterizadas através de espectroscopia UV-Visível, microscopia eletrônica de varredura (MEV), potencial zeta e dosagem de íons remanescentes após as reações. A atividade antimicrobiana tanto das nanopartículas como de suas formulações contra Candida albicans SC 5314 e Staphylococcus aureus ATCC 25923 foi avaliada por meio do método da microdiluição. ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The aim of this study was to investigate the production of silver nanoparticles through peel extract of pomegranate, and produce formulations containing these particles to be used in wound healings. Its antimicrobial action, cytotoxicity and healing potential were tested. The synthesis of silver nanoparticles were based on two methods proposed in the literature with some modifications, which were used carboxymethylcellulose, propylene glycol, silver nitrate, water and peel extract of pomegranate as reducing agent. The peel extract was characterized by pH, dry mass and bioactive tannins (elagic acid and total phenols expressed as galic acid). The total phenols were also quantified after being heated at 50ºC and 100ºC for 12 minutes, 1 hour and 2 hours. Then, silver nanoparticles were added in a solution containing products to develop a formulation to be tested in wound healing. They were characterized by UV-Vis spectroscopy, scanning electron microscopy (SEM), zeta potential and the quantification of remaining silver ions after the synthesis reaction. The antimicrobial activity of the nanoparticles and formulations were tested against Candida albicans (SC 5314) e Staphylococcus aureus (ATCC 25923) by microdilution method. After submitting the peel extract to different conditions of temperature and times (50ºC and 100ºC for 12 minutes, 1 hour and 2 hours), it was noted that the values of the minimum inhibitory concentration was not affected and were 391 μg/ml and 781 μg/ml for S. aureus and C. albicans. The formation of silver nanoparticles was confirmed through the formation of characteristic peaks in the UV-Vis spectroscopy and SEM images, and it was observed that the reaction at 50ºC for 12 min produced silver nanoparticles with regular forms and better dispersed in the formulation. The synthesis proposed promoted...
Doutor
Liesse, Iyamba Jean-Marie. "Etude de l'interaction des souches cliniques de Staphylococcus aureus avec une surface abiotique." Doctoral thesis, Universite Libre de Bruxelles, 2012. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209630.
Full textChez S. aureus, la formation du biofilm se déroule en deux phases principales: la première phase est l’attachement initial des cellules sur une surface, et la seconde est la multiplication et la formation d’une communauté structurée, mature et multicouche des cellules bactériennes. A l’intérieur du biofilm, les bactéries développent plusieurs types d’interactions et accroissent leur résistance aux agents antimicrobiens et aux défenses immunitaires de l’hôte, ce qui constitue un véritable problème de santé publique.
Les objectifs de ce travail étaient: (1) de caractériser des souches cliniques de S. aureus sensibles et résistantes à la méticilline (SASM et SARM) par une analyse phénotypique et génotypique; (2) d’étudier la répercussion des propriétés de membranes sur l’adhésion et la formation du biofilm; (3) de rechercher un moyen pour la prévention de l’adhésion et de la formation d’un biofilm sur une surface abiotique.
Deux souches de référence et 12 souches cliniques de S. aureus (4 SARM et 8 SASM) collectées à Kinshasa ont été caractérisées par la résistance aux antibiotiques, par le typage d’une région X du gène spa codant pour la protéine A de S. aureus et par la détermination des propriétés de la surface cellulaire. L’adhésion à une surface et la formation du biofilm ont été respectivement étudiées par la méthode de Biofilm Ring Test® (BFRT®) et par celle de coloration au cristal violet. Ces deux méthodes ont été utilisées pour l’évaluation de l’activité de l’acide éthylèneglycol tétraacétique (EGTA) sur l’adhésion et la formation du biofilm.
L’amplification par PCR (Polymerase Chain Reaction) d’un fragment du gène mecA a confirmé l’appartenance des souches étudiées au phénotype SARM ou SASM. L’analyse par PCR des répétitions présentes dans la séquence codante de la protéine A de S. aureus (spa typing) a permis d’identifier 7 types spa pour toutes les souches SARM et SASM2 dont un nouveau type spa t10715.
Les résultats du test MATS (Microbial Adhesion to Solvents) ont montré que les souches de S. aureus sensibles et résistantes à la méticilline possédaient des propriétés membranaires différentes susceptibles de modifier l’adhésion ou la formation d’un biofilm. Les souches sensibles à la méticilline avaient une paroi plus hydrophobe que celle de souches résistantes dont la paroi était acide, acceptrice d’électrons.
Les études sur l’interaction entre des souches cliniques de S. aureus et des surfaces abiotiques ont montré que les souches SARM adhéraient moins vite à une surface et formaient moins de biofilms que les souches SASM.
Les études de l’activité de l’EGTA, un chélateur des cations divalents, ont montré que ce dernier inhibait l’adhésion de souches SARM à une surface abiotique comme un tube de cathéter et empêchait la formation d’un biofilm par toutes les souches sensibles et résistantes à la méticilline. Cette action inhibitrice sur la formation du biofilm était réversible en présence d’un cation divalent (magnésium, calcium ou manganèse).
L’ensemble des données obtenues sur l’adhésion et la formation du biofilm par la méthode de BFRT® et par celle de coloration au cristal violet ont montré que le BFRT® était la méthode de choix dans les études de l’adhésion initiale des souches de S. aureus sur une surface abiotique. Le BFRT® pourrait être utilisée dans le screening rapide de produits contre l’adhésion bactérienne à la surface des implants médicaux à base de polystyrène ou de silicone.
Doctorat en Sciences biomédicales et pharmaceutiques
info:eu-repo/semantics/nonPublished
Vance, Lindsey. "The Inhibitory Effects of a Novel Gel on Staphylococcus aureus Biofilms." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/honors/435.
Full textVieira, Estevão Alan. "Ação antimicrobiana de ramnolipídeos sobre células sésseis e planctônicas de Staphylococcus aureus: efeito do pH." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/75/75133/tde-13032019-163150/.
Full textFood poisoning can be considered one of the most significant causes of mortality in developed and developing countries with bacterial contamination being responsible for the majority of cases. Among them are those caused by Staphylococcus species that can beharmful, both from the infection of the host organism as the intoxication by thermostable enterotoxins present in contaminated and poorly conditioned food. In addition, the ability to form biofilms gives S. aureus greater protection against control agents. Under this context, it becomes important to develop new methods to inhibit those pathogens. An alternative to the use of synthetic preservatives are biosurfactants such as rhamnolipids (RL), which shows high stability to temperature, pH and salt concentration along with the fact that they have low toxicity and are biodegradable. The aim of this study was to evaluate the effect of pH on the antimicrobial activity of RL on planktonic and sessile Staphylococcus aureus cells (ATCC 8095) and compare its effect to that of the sodium dodecyl sulfate (SDS). The results showed that pH exerts a great influence on the action of surfactants, with greater effectiveness at lower pH. At pH 5, RL presented higher bactericidal action (CBM = 19.5 mg L-1) than SDS (CBM = 39.1 mg L-1), also being able to inhibit and remove 80% of biofilms at concentrations of 156. 2 mg L- 1 and reduce the hydrophobicity of S. aureus cell surface by about 40%. At higher pH values, the action of RL did not exceed that of SDS, neverthelessthey showed promising results, mainly on the removal of biofilms evidenced by confocal microscopy. FTIR spectroscopy revealed that at pH 6, 7 and 8,S. aureus possibly induces changes in its cell membrane in order to decrease the sensitivity to surfactants. The MEV images showed that the RL cause deformations in the cells, which can lead to rupture. The increase in ionic strength, promoted by the addition of NaCl in the medium, favored the antimicrobial action of RL, which makes the application of RL in foods very promising.
Books on the topic "Staphylococcus aureus biofilms"
Hodgson, Anne Elizabeth. Biofilm-specific physiologies of staphylococcus aureus. Manchester: Universityof Manchester, 1994.
Find full textBook chapters on the topic "Staphylococcus aureus biofilms"
Rosenthal, Carolyn B., Joe M. Mootz, and Alexander R. Horswill. "Staphylococcus aureus Biofilm Formation and Inhibition." In Springer Series on Biofilms, 233–55. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-53833-9_11.
Full textMusini, Anjaneyulu, Sai Pavan Chilumoju, and Archana Giri. "Biofilm Formation in Drug-Resistant Pathogen Staphylococcus aureus." In Microbial Biofilms, 23–46. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003184942-3.
Full textArcher, Nathan K., J. William Costerton, Jeff G. Leid, and Mark E. Shirtliff. "Immunological Methods for Staphylococcus aureus Infection Diagnosis and Prevention." In Springer Series on Biofilms, 61–75. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-29554-6_5.
Full textSilva, Vanessa, José L. Capelo, Gilberto Igrejas, and Patrícia Poeta. "Molecular Mechanisms of Antimicrobial Resistance in Staphylococcus aureus Biofilms." In Emerging Modalities in Mitigation of Antimicrobial Resistance, 291–314. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-84126-3_12.
Full textKalia, Vipin Chandra, Shikha Koul, Subhasree Ray, and Jyotsana Prakash. "Targeting Quorum Sensing Mediated Staphylococcus aureus Biofilms: A Proteolytic Approach." In Biotechnological Applications of Quorum Sensing Inhibitors, 23–32. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-9026-4_2.
Full textRiool, Martijn, and Sebastian A. J. Zaat. "Biomaterial-Associated Infection: Pathogenesis and Prevention." In Urinary Stents, 245–57. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-04484-7_20.
Full textKumar, Sanjay, Simranjeet Singh, Vijay Kumar, Shivika Datta, Daljeet Singh Dhanjal, Priyanka Sharma, and Joginder Singh. "Pathogenesis and Antibiotic Resistance of Staphylococcus aureus." In Model Organisms for Microbial Pathogenesis, Biofilm Formation and Antimicrobial Drug Discovery, 99–115. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-1695-5_7.
Full textCassat, James E., Chia Y. Lee, and Mark S. Smeltzer. "Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus." In Methods in Molecular Biology, 127–44. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-468-1_10.
Full textCassat, James E., Mark S. Smeltzer, and Chia Y. Lee. "Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus." In Methods in Molecular Biology, 195–211. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-664-1_12.
Full textNoguera, M., O. Franquesa, D. Herrera, C. Cifuentes, R. Guix, T. Prenafeta, and R. March. "Efficacy of a Staphylococcus aureus biofilm-embedded bacterin against coagulase-negative staphylococci intramammary infections in dairy cows." In Udder Health and Communication, 381. Wageningen: Wageningen Academic Publishers, 2011. http://dx.doi.org/10.3920/978-90-8686-742-4_72.
Full textConference papers on the topic "Staphylococcus aureus biofilms"
Bigelow, Timothy A., Clayton Thomas, Huaiqing Wu, and Kamal Itani. "Impact of step size on histotripsy treatment of staphylococcus aureus biofilms on surgical mesh." In 2017 IEEE International Ultrasonics Symposium (IUS). IEEE, 2017. http://dx.doi.org/10.1109/ultsym.2017.8091780.
Full textBigelow, Timothy, Clayton Thomas, Huaiqing Wu, and Kamal Itani. "Impact of step size on histotripsy treatment of Staphylococcus aureus biofilms on surgical mesh." In 2017 IEEE International Ultrasonics Symposium (IUS). IEEE, 2017. http://dx.doi.org/10.1109/ultsym.2017.8092540.
Full textPradhana, A. A. S., S. D. Astuti, M. Khasanah, and R. K. D. Ardianti. "Detection of gas concentrations based on age on Staphylococcus aureus biofilms with gas array sensors." In THE 2ND INTERNATIONAL CONFERENCE ON PHYSICAL INSTRUMENTATION AND ADVANCED MATERIALS 2019. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0034112.
Full textOubekka, S. Daddi, R. Briandet, F. Waharte, M. P. Fontaine-Aupart, and K. Steenkeste. "Image-based Fluorescence Recovery After Photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms." In European Conference on Biomedical Optics. Washington, D.C.: OSA, 2011. http://dx.doi.org/10.1364/ecbo.2011.80871i.
Full textDaddi Oubekka, S., R. Briandet, F. Waharte, M. P. Fontaine-Aupart, and K. Steenkeste. "Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms." In European Conferences on Biomedical Optics, edited by Nirmala Ramanujam and Jürgen Popp. SPIE, 2011. http://dx.doi.org/10.1117/12.889461.
Full textFerreira, Maria Gabriela, DENISE VON DOLINGER DE BRITO RÖDER, MÁRIO PAULO AMANTE PENATTI, PRISCILA GUERINO VILELA ALVES, and RALCIANE DE PAULA MENEZES. "O QUE HÁ DE NOVO SOBRE A AÇÃO DE PRODUTOS NATURAIS NA INIBIÇÃO DA FORMAÇÃO DE BIOFILME POR ISOLADOS DE STAPHYLOCOCCUS AUREUS?" In II Congresso Nacional de Microbiologia Clínica On-line. Revista Multidisciplinar em Saúde, 2022. http://dx.doi.org/10.51161/ii-conamic/34.
Full textBrum, Natália Franco, éverton Patric Dos Santos Amaral, Leonardo Quintana Soares Lopes, and Patrícia Kolling Marquezan. "ATIVIDADE ANTIMICROBIANA E ANTIBIOFILME DO ÓLEO ESSENCIAL DE SALVIA ROSMARINUS FRENTE À BACTÉRIA STAPHYLOCOCCUS AUREUS." In III Congresso Brasileiro de Ciências Farmacêuticas On-line. Revista Multidisciplinar em Saúde, 2022. http://dx.doi.org/10.51161/conbracif/62.
Full textViana, Marcelino Gevilbergue, João Pedro Reinaldo De Araújo, and Louise Duarte Matias de Amorim. "Inibição do crescimento de Staphylococcus aureus em biofilme por ação do extrato bruto do metabólito de Fusarium sp." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1159.
Full textA. Rashid, Sirwan, Sawsan Muhammed Sorchee, Mustafa D. Yonus, and Omar F. Bahjat. "Identification Biofilm Producers s. Aureus Isolates and Detect their Biofilm Genes from Gingivitis Cases." In 4th International Conference on Biological & Health Sciences (CIC-BIOHS’2022). Cihan University, 2022. http://dx.doi.org/10.24086/biohs2022/paper.586.
Full textLins, Nathalia Alexandre Eloy, Maria Carolina Oliveira Lins, Renan Lennon Silva Henrique, Pettely Thaíse De Souza Santos Palmeira, Maria Helena Chaves de Vasconcelos Catão, Sérgio de Lemos Campello, Anderson Stevens Leônidas Gomes, Patrícia Lins Azevedo do Nascimento, and Cláudia Cristina Brainer de Oliveira Mota. "Analysis of biofilm growth over bulk fill composite resins through optical coherence tomography." In Latin America Optics and Photonics Conference. Washington, D.C.: Optica Publishing Group, 2022. http://dx.doi.org/10.1364/laop.2022.tu1b.4.
Full textReports on the topic "Staphylococcus aureus biofilms"
Rahimipour, Shai, and David Donovan. Renewable, long-term, antimicrobial surface treatments through dopamine-mediated binding of peptidoglycan hydrolases. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597930.bard.
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