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Academic literature on the topic 'Stallion sperm'

Author: Grafiati
Published: 10 December 2022
Last updated: 29 January 2023

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Journal articles on the topic "Stallion sperm"

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Miró, Jordi, and Marion Papas. "Improvement of cryopreservation protocol in both purebred horses including Spanish horses." Spanish Journal of Agricultural Research 16, no. 4 (January 8, 2019): e0406. http://dx.doi.org/10.5424/sjar/2018164-13677.

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There is a widely held belief that the semen of Purebred Spanish Horses (PRE) is of generally poorer quality than that of other breeds, and survives cryopreservation less well. To determine whether this is the case, sperm concentration, viability and morphological abnormalities were examined in a total 610 fresh ejaculates from 64 healthy PRE (N=47) and non-PRE stallions (N=17). Sperm concentration and viability were then re-examined after pre-freezing centrifugation, and once again after freezing-thawing. No differences were observed between the PRE and non-PRE stallions in terms of any sperm quality variable at any observation point. When considering all PRE and non-PRE samples together, differences in sperm viability were observed between fresh and fresh-centrifuged sperm viability (70.1±12.5% compared to 76.3±10.9%; p<0.01). After centrifugation the samples were also more homogeneous in terms of the total number of recovered sperm cells. Centrifugation also improved frozen-thawed sperm viability, reducing differences in sperm quality between individual stallions. For all centrifugations, a sperm:extender ratio of 1:5 was used. This would appear to provide better final results than those reported in the literature for the 1:1 ratio commonly used for PRE stallion sperm cryopreservation. In conclusion, obtained results show that the quality and frozen/thawed results of PRE stallion sperm are not lower than that of non-PRE breeds. In addition, using a 1:5 sperm:extender dilution ratio when selecting sperms by centrifugation prior to freezing, seems to provide better results than those usually reported when using a 1:1 ratio.
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Chaudhary, A. K., G. N. Purohit, J. S. Mehta, S. K. Ravi, and T. R. Talluri. "144 Serum testosterone profile in Marwari stallions and its relationship with testicular parameters, semen characteristics, reaction time, stallion age, bodyweight, and height." Reproduction, Fertility and Development 32, no. 2 (2020): 198. http://dx.doi.org/10.1071/rdv32n2ab144.

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The present study investigated the serum testosterone profile of Marwari stallions before and after exposure to a mare and the relationship of serum testosterone profile with scrotal circumference, semen characteristics, reaction time, stallion age, bodyweight, and height. Marwari stallions (n=9) of three age groups (2-4 years, n=3; 4-6 years, n=3; and 6+ years, n=3) were used in the study. Scrotal circumference, height, and bodyweight of each of the stallions were measured. Semen was collected from each stallion twice per week in the early-morning hours using an artificial vagina and a mare in oestrus. Six ejaculates were collected from each stallion for evaluation of various seminal parameters (semen volume, sperm concentration, and progressive sperm motility). Reaction time for each stallion was also recorded. At every alternate semen collection (first, third, and fifth collections), blood samples were taken 15min before exposure to the mare and just before the semen collection for serum testosterone hormone assay using a horse testosterone enzyme-linked immunosorbent assay kit. The data obtained were analysed statistically using SPSS ver. 20.0 (IBM Corp.). The results showed that mean testosterone concentration was significantly different (P ≤ 0.01) among the stallions and was significantly lower (P ≤ 0.01) in the stallions below 4 years of age. No significant difference in testosterone level was observed before and after exposure to a mare. A positive correlation was detected between testosterone and both scrotal circumference (P ≤ 0.05) and sperm concentration (P ≤ 0.05), whereas a negative correlation was observed with reaction time (P ≤ 0.01). It was concluded that exposure to a mare does not change the testosterone level in stallion blood and that there is a relationship of serum testosterone concentration with scrotal circumference, sperm concentration, reaction time, and age but not with height and bodyweight.
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Nikitkina, E. V., A. A. Krutikova, and A. A. Musidray. "GRM8 GENE POLYMORPHISM AND STALLION SPERM QUALITY." International Journal of Veterinary Medicine, no. 3 (October 17, 2022): 200–203. http://dx.doi.org/10.52419/issn2072-2419.2022.3.200.

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Genome-Wide Association Studies fertility will allow further selection of animals at the genomic level, and genomic selection will allow the selection of animals with good spermatogenesis at an early age. After our GWAS, several candidate genes associated with stallion sperm quality were identified. One of these genes was the GRM8 gene. In the course of Sanger sequencing studies, four SNPs were identified in the exon of the GRM8 gene and their association with the quality of stallion sperm was carried out. For the rs1138419111 genotype, no significant differences were found in the studied parameters. According to the identified single nucleotide substitution rs1147388106, the largest volume of ejaculate was in stallions with the GG genotype. According to SNP rs395286150, stallions with the heterozygous CT genotype had the best sperm quality. Analysis of data on the SNP rs394524550 revealed a significant effect of the genotype on progressive motility. Three of the four SNPs identified in the exon of the GRM8 gene are significantly associated with such indicators of stallion sperm quality as ejaculate volume, concentration, and progressive motility. Project of the Ministry of Education and Science No. 121052600354-7.
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Heise, A., D. Gerber, D. H. Volkmann, W. Kähn, and N. K. Brouwer. "11 FERTILITY OF FRESH AND FROZEN - THAWED EPIDIDYMAL STALLION SPERM WITH OR WITHOUT EXPOSURE TO SEMINAL PLASMA." Reproduction, Fertility and Development 19, no. 1 (2007): 124. http://dx.doi.org/10.1071/rdv19n1ab11.

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The aim of this study was to determine if the addition of equine seminal plasma to epididymal semen enhances its fertility before or after freezing. Thirty-two mares were randomly assigned to 5 stallions; 3 stallions were kept in Pretoria, each having 7 mares, and 2 stallions were kept at Cornell, one having 6 mares and the other 5. Mares were synchronized using 10 daily IM progesterone and estradiol injections; an Ovuplant® implant (26 mg of deslorelin; Peptech Animal Health, Sydney, NSW, Australia) was inserted under the mucosa of the vaginal vestibulum once a follicle reached a diameter of 35 mm; implants were removed after ovulation. Mares were inseminated 30 h after implant insertion. Each insemination dose consisted of 200 million progressively motile sperm and was deposited into the uterine body. Following insemination, mares were examined for ovulation at 6 hourly intervals. Fourteen days after ovulation, mares were examined for pregnancy by transrectal ultrasonography and treated with PGF2α to induce the next estrus. Seminal plasma was collected from the stallions used in the trial prior to castration, frozen, and stored. In Pretoria, stallions were castrated and one epididymal tail was flushed with seminal plasma and the other with skim milk extender; in the first cycle, half of the mares were inseminated with one of the two sperm samples. In Cornell, testes of each stallion were removed 3 weeks apart, and all mares were inseminated first with one and 3 weeks later with the other semen sample. Mares were inseminated during consecutive estrous cycles using the following sperm types: fresh epididymal sperm that had been exposed to seminal plasma (G1: 4 mares per stallion in Pretoria, 6 and 5 mares per stallion at Cornell); fresh epididymal sperm that had never been exposed to seminal plasma (G2: 3 mares per stallion in Pretoria, 6 and 5 mares per stallion at Cornell); frozen–thawed ejaculated sperm (G3); frozen–thawed epididymal sperm that had been exposed to seminal plasma prior to freezing (G4); and frozen–thawed epididymal sperm that had never been exposed to seminal plasma (G5). The results of inseminations with fresh epididymal semen (G1–2) of 5 stallions and the preliminary results of inseminations with frozen–thawed epididymal semen (G3–5) of 2 stallions are summarized in the Table 1. Cycles where ovulation did not occur within 12 h after insemination were excluded. The pregnancy rate of mares inseminated with fresh epididymal sperm of G1 was significantly higher (chi-square test; P &lt; 0.05) than that of mares of G2. The pregnancy rate of mares inseminated with frozen–thawed ejaculated semen (G3) was similar to that of mares inseminated with frozen–thawed epididymal semen of G4 and G5 (P = 0.3). Based on these preliminary results, we conclude that the fertility of fresh epididymal sperm can be enhanced by exposure to equine seminal plasma. To determine if the same holds true for frozen–thawed epididymal sperm, more inseminations must be performed. Table 1.Results of inseminations with various semen types
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Pessoa, G. A., J. M. Trentin, A. P. Martini, D. R. Dotto, L. A. M. Centeno, M. L. Jardim, K. V. Aires, and M. I. B. Rubin. "180 SPERM SELECTION OF STALLION PONIES THROUGH GLASS WOOL." Reproduction, Fertility and Development 26, no. 1 (2014): 204. http://dx.doi.org/10.1071/rdv26n1ab180.

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Two techniques of sperm concentration (centrifugation or filtering) and sperm separation technique with glass wool were applied to the sperm samples collected from 3 pony stallions (6 ejaculates; 2 from each stallion). Ejaculates were extended to a final concentration of 50 × 106 spermatozoa mL–1 using a nonfat dry milk-based extender and evaluations occurred at 24, 48, and 72 h after immediate ejaculate dilution and cooling. Each stallion was considered as a block, and semen from each stallion was assigned to 4 treatments: Group A (control): extended semen alone; Group B: extended-centrifuged semen; Group C: extended-sperm filtered semen; Group D: extended-glass wool-separated semen. All groups were tested for pH, osmolarity, motility, morphology, membrane functionality (hyposmotic), and cell viability (MTT assay). The experimental design was performed using a split-plot model. Data analysis at the level of 5% was performed using ANOVA and Bonferroni as post-hoc test. Data are presented as mean ± standard error. Group D had the highest rate of viable cells (P < 0.05) after the separation procedure (Table 1). Group B had a higher percentage of cells with tail defects after processing compared with the controls and Groups A, C, and D (P < 0.05). More than 60% of the cells retained on the filter showed defects (P < 0.001). Progressive motility was greater in group D at 0, 24, and 48 h (P < 0.05). Seventy-two hours after cooling, motility in groups A and B was lower than in Group D (P < 0.01). Group D showed a higher number of cells with mitochondrial activity during the cooling period. In conclusion, the technique of sperm selection by gravity using a glass wool filter resulted in an increased number of viable sperms after cooling pony semen for 24, 48, and 72 h. Table 1.Effect of sperm concentration and separation techniques on mean ± standard error percent of intact sperm from 3 stallions ponies (2 ejaculates/pony) stained with eosin-nigrosin
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Severa, L., L. Máchal, I. Křivánek, M. Machatková, and O. Mamica. "Characteristic of selected rheological parameters of stallion ejaculate." Archives Animal Breeding 51, no. 1 (October 10, 2008): 16–22. http://dx.doi.org/10.5194/aab-51-16-2008.

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Abstract. Dynamic viscosity of native (30 minutes after ejaculation) and 24 hours stored (at 4 °C) stallion ejaculate was measured. The ejaculate from 10 breeding stallions was examined in three different experimental series. The average value of dynamic viscosity at shear rate 1.02 s−1 was found to be 416.8 ± 10.1 mPa.s. The correlation between ejaculate volume, sperm concentration and viscosity was tested. The experiments resulted in finding a dependence between increasing viscosity and decreasing sperm concentration (rp = −0.67; P<0.05). Performed experiments with changing shear rate demonstrated non-Newtonian characteristics of stallion ejaculate with a clear shear-thinning behaviour. Stallion ejaculate appeared to be slightly time-dependent liquid.
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Oldenhof, Harriëtte, Anna Heutelbeck, Anne-Kathrin Blässe, Heinrich Bollwein, Gunilla Martinsson, Willem F. Wolkers, and Harald Sieme. "Tolerance of spermatozoa to hypotonic stress: role of membrane fluidity and correlation with cryosurvival." Reproduction, Fertility and Development 27, no. 2 (2015): 285. http://dx.doi.org/10.1071/rd13177.

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The aim of this study was to evaluate inter-individual variability in osmotic properties of stallion spermatozoa and its correlation with cryosurvival. In addition, temperature dependency of hypo-osmotic tolerance and membrane fluidity were studied. Stallion sperm membranes exhibited good resistance towards hypotonic stress in the 15–30°C temperature range, whereas membrane stability was found to be decreased at 4 and 37°C. Bull spermatozoa showed greater hypo-osmotic tolerance compared with stallion spermatozoa, especially at temperatures above 30°C, which coincided with decreased membrane fluidity of bovine spermatozoa in this temperature range. The critical osmolality at 22°C, at which half of the sperm population survived exposure to hypotonic saline solution, was found to vary between 55 and 170 mOsm kg–1 among different stallions. Clear correlations were found for pre- versus post-freeze sperm motility and membrane integrity. Pre-freeze percentages of membrane-intact spermatozoa after exposure to hypotonic stress showed a weak correlation with sperm motility after cryopreservation. This correlation, however, was not found when data were corrected for initial numbers of membrane-intact spermatozoa in the sample. We thus conclude that studies on pre-freeze tolerance towards hypotonic stress cannot be used to predict sperm cryosurvival rates for individual stallions.
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RAVAL, KATHAN, NILENDU PAUL, PRADEEP NAG, ELANGO K, YASH PAL, LEGHA R A, T. R. TALLURI, and ARUMUGAM KUMARESAN. "Asthenozoospermic stallions tend to have high acrosome reacted spermatozoa as evidenced by dual fluorescent staining assay." Indian Journal of Animal Sciences 92, no. 8 (August 22, 2022): 946–49. http://dx.doi.org/10.56093/ijans.v92i8.120018.

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Acrosome intactness of spermatozoa is the critical factor for establishing sperm reservoir in oviduct and for fertilizing an oocyte. However, frozen thawed spermatozoa tend to show higher proportion of acrosome reacted spermatozoa thereby compromising the fertility. Conventional staining techniques identify only sperm acrosome integrity and not precisely the acrosome reaction status. In this context, the current study was conducted to assess the acrosome status of cryopreserved spermatozoa using Fluorescein isothiocyanate conjugated peanut agglutinin and propidium iodide (FITC-PNA+PI) in stallions with varying sperm motility. Stallions were classified into high- (≥45%) and low-motile group (≤30%) based on their post-thaw sperm motility. The proportion of live acrosome intact (LAI) spermatozoa was significantly higher in high-motile group as compared to low-motile group. A significant positive correlation was observed between LAI and post-thaw sperm motility. In conclusion, the present study showed that FITC-PNA+PI combination could be used for rapid and accurate assessment of acrosome reaction status of stallion spermatozoa, and the proportion of LAI population in cryopreserved stallion semen had a strong correlation with sperm motility.
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Monteiro, G. A., P. N. Guasti, F. P. Hartwig, J. A. Dellaqua Jr., M. A. Alvarenga, and F. O. Papa. "Cooling of ejaculated and epididymal stallion sperm." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 65, no. 3 (June 2013): 681–86. http://dx.doi.org/10.1590/s0102-09352013000300010.

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After a serious injury or sudden death, epididymis cauda sperm recovery and cryopreservation may present as the last opportunity to obtain genetic material from a valuable stallion. This study evaluated the viability of cooled equine sperm collected by three different methods: sperm of ejaculated (G1), sperm recovered from the epididymal cauda immediately after orchiectomy (G2) and sperm recovered from the epididymal cauda after storage for 24 hours at 5°C (G3). To obtain G1 sperm, two ejaculates were collected. After 1 week, all stallions underwent a bilateral orchiectomy, and one of the removed epididymides was flushed to obtain G2 sperm. The contralateral epididymis was stored at 5°C for 24 hours before being flushed to obtain G3 sperm. The sperm samples were evaluated immediately after the addition of the refrigeration extender, and after 24 and 48 hours of storage at 5°C. After 24 and 48 hours of storage, the epididymal sperm demonstrated higher motility traits when compared to the ejaculated sperm (P<0.05). These results indicate that sperm recovered from the epididymal cauda of stallions are more resistant to the cooling process, with higher kinetic parameters and plasma membrane integrity when compared to the ejaculated sperm.
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TKACHEV, A. V., YU I. KOROVIN, and O. L. TKACHEVA. "CRYORESISTANCE OF STALLION SPERM IN DIFFERENT MONTHS OF THE YEAR." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 2 (2022): 79–87. http://dx.doi.org/10.26897/0021-342x-2022-2-79-87.

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It is known that the seasonality of reproductive function is much more pronounced in mares than in stallions. The mare goes through an anestrus period in autumn and winter, while spermatogenesis in stallions continues throughout the year. Therefore, studies of cryoresistance of stallion sperm in different months of the year are relevant. The aim of the study was to study the cryoresistance of stallion sperm in different months of the year when creating cryopreservation of genetic material with intensive breeding use of sires. The cryoresistance of the sperm of stallions with their intensive breeding use was the best from September to December during sexual rest. The highest incidence of sperm was in December, which is 0.44% more than in October, 5.12% more than in November, 7.62% more (P<0.05) than in September, 20.53% more (P<0.01) than in January, 27.3% more (P<0.01) compared to February, 52.7% more (P<0.001) than in March, 137.9% more (P<0.05) than in April, 34.12% more (P<0.001) than in May, 23.5% more (P<0.001) than in June and 59.7% more (P<0.001) than in July. The survivability of sperms after thawing in a thermostat at 37°C for less than 3 hours was observed in March, April and July. The lowest sperm motility after defrosting was observed in April, which is 36.4% less (P<0.001) than in July, 38.6% less (P<0.001) than in May, 45.12% less (P<0.001) than in June, 50.1% less (P<0.001) than in in January, 52.6% less (P<0.001) than in February, 43.75% less (P<0.001) than in March, 53.8% less (P<0.001) than in September, 55% less (P<0.001) than in October, 54.4% less (P<0.001) than in November and 54.1% less (P<0.001) than in December. Based on the data obtained on the mobility of deconserved sperm doses, we mat expect the highest fertility from the genetic material that is harvested from September to December.
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Dissertations / Theses on the topic "Stallion sperm"

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Schütze, Saskia [Verfasser]. "DNA stability of stallion sperm: Factors affecting chromatin integrity in individual stallions / Saskia Schütze." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2015. http://d-nb.info/1080868305/34.

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Waite, Jessica Arlene. "Cushioned centrifugation of stallion semen: factors impacting equine sperm recovery rate and quality." Thesis, Texas A&M University, 2007. http://hdl.handle.net/1969.1/85886.

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Centrifugation of stallion semen is an integral part of the cryopreservation procedure, primarily allowing for the concentration of sperm and removal of seminal plasma. In addition, centrifugation is required for maximizing spermatozoal quality in semen from some stallions subjected to cooled transport, because of the detrimental effects of long-term exposure to high levels of seminal plasma. The centrifugation process, however, has potential deleterious effects, including reduction in sperm quality as well as loss of sperm numbers. Since centrifugation plays such a crucial role in semen processing, two experiments were designed to evaluate more efficient centrifugation methods to meet the demands of the equine industry. In Experiment 1, semen was centrifuged in two different tube types (nipple- or conical-bottom), using a cushioned technique (Eqcellsire® Component B) with two different extenders (opaque-INRA96 or clear-HGLL). For Experiment 2, nipple-tube centrifugation was conducted at two different g forces (400 or 600) for 20 min, using three different iodixanol cushion media, Eqcellsire® Component B, OptiPrep[TM], or Cushion Fluid[TM]. Regardless of tube or extender types, centrifugation of semen resulted in sperm recovery rates ≥90%; however, centrifugation in INRA 96 extender yielded higher sperm motility values than did centrifugation in HGLL extender (P < 0.05). Cushion type or g force did not impact post-centrifugation semen quality, based on the laboratory values measured (P > 0.05). These results indicate that cushioned centrifugation of stallion semen in either conical-bottom or nipple-bottom tubes can yield a high sperm harvest, while maintaining sperm function. An optically opaque extender, as is typically used in the equine breeding industry, can be used to achieve this goal. The fertility rate (94%; 131/140) following cushioned semen centrifugation in a commercial program this past year indicates that these laboratory results are transferable to the clinical setting.
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Ketchum, Chelsea C. "Identification of Sperm Chromatin Proteins as Candidate Markers of Stallion Fertility." DigitalCommons@USU, 2018. https://digitalcommons.usu.edu/etd/7345.

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During spermatogenesis, histones are largely replaced by transition proteins and protamines in normal stallions. Incomplete nucleoprotein exchange results in the abnormal retention of histones and transition proteins, which is an indicator of poor sperm quality. Equine nucleoprotein exchange has not previously been investigated in detail, so that equine sperm chromatin quality problems, which are often responsible for poor breeding performance of stallions, are not well understood. In order to characterize chromatin remodeling events in stallion spermatogenesis and to identify proteins indicative of sperm chromatin defects, such as excessive amounts of histones, we identified antibodies that recognize equine testis-specific proteins of interest. Immunoblotting of testis and sperm protein lysates and immunofluorescence staining of histological tissue sections were used to identify candidate marker proteins of incomplete sperm chromatin maturation. Results of the study, which represents the first comprehensive characterization of the nucleoprotein exchange during spermatogenesis in the stallion, challenge the paradigm that the main function of histone H4 lysine (hyper-) acetylation (concomitant H4K5 and H4K8 acetylation) is to facilitate nucleosome ejection during spermatid nuclear elongation to allow for transition protein and protamine insertion into the chromatin. That paradigm was based on observations in mice and rats where H4 acetylation in several lysine residues occurs just prior to or during nuclear elongation. In contrast, the equine data presented here show strong acetylation of H4 in K5, K8 and K12 positions immediately after meiosis in round spermatids, independent of nuclear transition protein 1 deposition. Furthermore, results of H4K16 acetylation analyses underline the importance of this mark, which is likely mediated by DNA damage signaling pathways, emphasizing the importance of DNA repair processes for the exchange of nucleoprotein exchange in spermiogenesis and therefore, in extension, for male fertility. In addition, a revised description of the equine spermatogenic cycle is proposed here that is better aligned with human, mouse and rat spermatogenesis. Finally, the testis-specific histone variant TH2B was identified as a potential quantitative marker of equine sperm quality.
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Heidmiller, Melodee Kathleen. "Characterization of the equine spermadhesin HSP-7 found on stallion spermatozoa as it relates to stallion fertility and sperm capacitation." DigitalCommons@CalPoly, 2011. https://digitalcommons.calpoly.edu/theses/484.

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Equine spermadhesin HSP-7 is a 14 kDa protein isolated from stallion seminal plasma and present on the surface of spermatozoa. HSP-7 displays carbohydrate and zona-pellucida binding properties, but the physiological role in equine fertilization is not well defined. HSP-7 has 98% amino acid sequence homology with the well-studied boar spermadhesin, AWN. Currently, these two proteins are considered to have the same reproductive function. Immunofluorescence studies presented here show that the stallion and boar spermadhesins are localized to different segments on spermatozoa. The variation in molecular compartmentalization of spermadhesin molecules in different species suggests that these structurally related proteins could be involved in independent events of fertilization. While the variation in HSP-7 abundance was not statistically significant between fertile and subfertile stallions, capacitated spermatozoa displayed a marked increase in HSP-7 when compared to neat sperm (P < 0.05). These results indicate that rather than aiding in capacitation, HSP-7 is exposed with capacitation and may have a more significant role in the acrosome reaction and sperm-oocyte recognition than previously documented.
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Dawson, George Ray. "LOCALIZATION ON SPERM, QUANTIFICATION AND MOLECULAR FEATURES OF TWO SEMINAL PROTEINS." Diss., Tucson, Arizona : University of Arizona, 2005. http://etd.library.arizona.edu/etd/GetFileServlet?file=file:///data1/pdf/etd/azu%5Fetd%5F1047%5F1%5Fm.pdf&type=application/pdf.

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Ertmer, Franziska [Verfasser]. "Induced oxidative stress in stallion sperm: effects on osmotic resistance and cryosurvival / Franziska Ertmer." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2016. http://d-nb.info/1124565574/34.

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Khan, Mohd Azam Khan bin Goriman. "Studies on capacitation and the effects of cooling and low temperature storage on stallion sperm function." Thesis, University of Glasgow, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244254.

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Heutelbeck, Anna [Verfasser]. "Cryopreservation of stallion sperm: correlating hypo-osmotic resistance and cryosurvival, and use of density centrifugation for delayed cryopreservation / Anna Heutelbeck." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2013. http://d-nb.info/1037830989/34.

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Canuto, Lucas Emanuel Ferreira. "Efeito do plasma seminal sobre a ligação de espermatozoides da cauda do epidídimo equino aos explantes da tuba uterina." Botucatu, 2019. http://hdl.handle.net/11449/182003.

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Orientador: Frederico Ozanam Papa
Resumo: A recuperação de espermatozoides da cauda do epidídimo é uma das principais alternativas nos casos de óbito inesperado, eutanásia, processos obstrutivos ou castração terapêutica. Nessa técnica os espermatozoides não entram em contato com o plasma seminal, não incorporando seus constituintes, que interferem nos processos fisiológicos importantes para fertilização, como a ligação dos espermatozoides ao reservatório da tuba uterina, que aumenta a vida útil do espermatozoide e diminui as chances de poliespermia. Nesse sentido o objetivo do presente trabalho foi comparar a cinética e ligação da tuba uterina aos espermatozoides recuperados da cauda do epidídimo com e sem adição de plasma seminal. Utilizou-se 8 garanhões da raça Minihorse para o resgate de espermatozoides da cauda do epidídimo pela técnica de fluxo retrógrado. Após a colheita foi dividido em 4 grupos, LD apenas com diluente a base de leite desnatado, GO recebeu adição de diluente para congelação a base de gema de ovo, PSB plasma seminal de garanhão com alta fertilidade e capacidade de refrigeração, e PSR plasma seminal de garanhão com fertilidade e capacidade de refrigeração inferiores. Foi avaliado cinética, integridade de membrana espermática e a taxa de ligação à explantes da tuba uterina. Não apresentaram diferença na integridade da membrana nem na taxa de ligação, no entanto, observou-se diferença quanto a cinética espermática. Conclui-se que a adição de plasma seminal de diferentes garanhões interferiu, de for... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Sperm retrieval from the tail of the epididymis is one of the main alternatives in cases of unexpected death, euthanasia, obstructive processes, therapeutic castration. In this technique spermatozoa do not come into contact with the seminal plasma, not incorporating their constituents, which interfere in the physiological processes important for fertilization, such as sperm binding to the uterine tube reservoir, which increases sperm life and decreases the chances of polyspermia. In this sense, the objective of the present study was to compare the kinetics and uterine tubal attachment to the spermatozoa recovered from the tail of the epididymis with and without addition of seminal plasma. Eight minihorse stallions were used to retrieve spermatozoa from the tail of the epididymis by the retrograde flow technique. After harvesting was divided into 4 groups, LD only with diluent the skim milk base, GO received addition of diluent for freezing the egg yolk, PSB seminal stallion plasma with high fertility and cooling capacity, and PSR seminal plasma fertility and lower cooling capacity. Kinetics, spermatic membrane integrity and the rate of binding to the explants of the uterine tube were evaluated. There was no difference in membrane integrity or binding rate, however, a difference was observed in spermatic kinetics. It was concluded that the addition of seminal plasma of different stallions interfered, differently in spermatozoa kinetics of the tail of the epididymis increased t... (Complete abstract click electronic access below)
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Lühr, Janina [Verfasser], Harald [Akademischer Betreuer] Sieme, and Hariëtte [Akademischer Betreuer] Oldenhof. "Evaluation of fertilization capacity of cryopreserved stallion sperm, directly after thawing and after cooled storage / Janina Lühr ; Harald Sieme, Hariëtte Oldenhof." Hannover : Stiftung Tierärztliche Hochschule Hannover, 2018. http://d-nb.info/117923717X/34.

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Books on the topic "Stallion sperm"

1

Murphy, Ronan P. Effect of deglycerolization following thawing on aspects of sperm viability in the stallion. Dublin: University College Dublin, 1996.

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Conference papers on the topic "Stallion sperm"

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ATROSHCHENKO, Mikhail M., Maria G. ENGALYCHEVA, Anna M. KUDLAEVA, and Elena Y. BORODKINA. "Effect of Blood Enzyme Activity on Stallion Sperm Quality and Cryostability." In Current Trends of Agricultural Industry in Global Economy. SibAC, 2021. http://dx.doi.org/10.32743/agri.gl.econ.2020.1-8.

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KALASHNIKOV, Valeriy V., Mikhail M. ATROSHCHENKO, Aleksandr M. ZAITSEV, Anastasia A. NEZALENOVA, and Andrey A. DATSYSHIN. "Correlation Between the Sperm Quality of Stallions and the Macronutrient Concentration in the Blood Serum." In Current Trends of Agricultural Industry in Global Economy. SibAC, 2021. http://dx.doi.org/10.32743/agri.gl.econ.2020.112-117.

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You might also be interested in the extended bibliographies on the topic 'Stallion sperm' for particular source types:
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