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1

Rosenblatt, J., and R. R. Wolfe. "Calculation of substrate flux using stable isotopes." American Journal of Physiology-Endocrinology and Metabolism 254, no. 4 (1988): E526—E531. http://dx.doi.org/10.1152/ajpendo.1988.254.4.e526.

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The use of stable isotope tracers to calculate substrate kinetics in humans is favored over the use of radioactive isotopes because of their greater safety and versatility. However, potential complications not met when dealing with radioactive tracers are caused by 1) the natural occurrence of the stable isotope used as a tracer and 2) the necessity to administer the tracer in an amount that cannot be treated as "massless." We therefore found it desirable to derive a theoretically valid equation for calculating the rate of appearance, Ra, of a substrate under steady-state conditions using a st
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2

Patterson, Kristine Y., and Claude Veillon. "Stable Isotopes of Minerals as Metabolic Tracers in Human Nutrition Research." Experimental Biology and Medicine 226, no. 4 (2001): 271–82. http://dx.doi.org/10.1177/153537020122600403.

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Enriched stable isotopes used as tracers have proven to be valuable in studies of the absorption and metabolism of minerals. Unlike radioisotopes, they can be used in high-risk population groups such as infants, children, and pregnant or lactating women. Estimates of mineral absorption can be made from the oral administration of a single tracer or from two tracers, one given orally and the other intravenously (IV). It is possible to determine the metabolism of the mineral with modeling based on the amount of the tracer or tracers in different biological samples. One of the key decisions in stu
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3

Cobelli, C., G. Toffolo, D. M. Bier, and R. Nosadini. "Models to interpret kinetic data in stable isotope tracer studies." American Journal of Physiology-Endocrinology and Metabolism 253, no. 5 (1987): E551—E564. http://dx.doi.org/10.1152/ajpendo.1987.253.5.e551.

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In contrast to "weightless" radioactive tracers, stable isotope tracers have nonnegligible mass and are naturally present in the system, and the measured variable is a ratio of two isotopic species. These features do not allow stable isotopic tracer data analysis using straightforward analogy with radioactive tracer approaches, even though this practice is common. In this study, we present kinetic variables, models, and measurements for the analysis and interpretation of stable isotope tracer data. Assumptions and mathematical techniques for modeling the data when perturbation is both nonnegli
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4

Kelleher, J. K., and T. M. Masterson. "Model equations for condensation biosynthesis using stable isotopes and radioisotopes." American Journal of Physiology-Endocrinology and Metabolism 262, no. 1 (1992): E118—E125. http://dx.doi.org/10.1152/ajpendo.1992.262.1.e118.

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Important syntheses in living systems occur by condensation reactions of the type nA----1B (where n is the number of A molecules needed to synthesize 1 molecule of B). Quantitative relationships for estimating the rate of synthesis of B from radioactive and stable isotope tracers are compared. With radioisotope tracers, only a single quantity is detected, the amount of radioactivity in B. In contrast, isotopes of varying mass produce multiple mass isotopomers B that are detected using mass spectrometry. The analysis demonstrates that the rate of synthesis of B is identifiable from stable isoto
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5

Hungate, Bruce A., Rebecca L. Mau, Egbert Schwartz, et al. "Quantitative Microbial Ecology through Stable Isotope Probing." Applied and Environmental Microbiology 81, no. 21 (2015): 7570–81. http://dx.doi.org/10.1128/aem.02280-15.

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ABSTRACTBacteria grow and transform elements at different rates, and as yet, quantifying this variation in the environment is difficult. Determining isotope enrichment with fine taxonomic resolution after exposure to isotope tracers could help, but there are few suitable techniques. We propose a modification tostableisotopeprobing (SIP) that enables the isotopic composition of DNA from individual bacterial taxa after exposure to isotope tracers to be determined. In our modification, after isopycnic centrifugation, DNA is collected in multiple density fractions, and each fraction is sequenced s
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6

Jensen, Alexandria, William Ford, James Fox, and Admin Husic. "Improving In-Stream Nutrient Routines in Water Quality Models Using Stable Isotope Tracers: A Review and Synthesis." Transactions of the ASABE 61, no. 1 (2018): 139–57. http://dx.doi.org/10.13031/trans.12545.

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Abstract. Water quality models serve as an economically feasible alternative to quantify fluxes of nutrient pollution and to simulate effective mitigation strategies; however, their applicability is often questioned due to broad uncertainties in model structure and parameterization, leading to uncertain outputs. We argue that reduction of uncertainty is partially achieved by integrating stable isotope data streams within the water quality model architecture. This article outlines the use of stable isotopes as a response variable within water quality models to improve the model boundary conditi
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7

Ludvigson, Greg A., Aaron F. Diefendorf, Marina B. Suarez, et al. "Stable Isotope Tracers of Cretaceous Arctic Paleoprecipitation." Geosciences 12, no. 4 (2022): 143. http://dx.doi.org/10.3390/geosciences12040143.

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We report estimated stable isotope compositions of depositional waters and paleoprecipitation from the Cretaceous Arctic to further elucidate the role of the global hydrologic cycle in sustaining polar warmth during that period. Estimates are based on new hydrogen isotopic analyses of n-alkane biomarkers extracted from Late Cretaceous and mid-Cretaceous terrestrial deposits in northern Alaska and the Canadian High Arctic. We integrate these new results with earlier published work on oxygen isotopic analyses of pedogenic siderites, dinosaurian tooth enamel phosphates, and pedogenic clay mineral
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8

Coggan, Andrew R. "Use of stable isotopes to study carbohydrate and fat metabolism at the whole-body level." Proceedings of the Nutrition Society 58, no. 4 (1999): 953–61. http://dx.doi.org/10.1017/s0029665199001263.

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The present review discusses the advantages and limitations of using stable-isotope tracers to assess carbohydrate and fat metabolism at the whole-body level. One advantage of stable-(v. radioactive-) isotope tracers is the relative ease with which the location of a label within a molecule can be determined using selected-ion-monitoring GC-mass spectrometry (SIM-GC- MS). This technique minimizes potential problems due to label recycling, allows the use of multiple-labelled compounds simultaneously (e.g. to quantify glucose cycling), and perhaps most importantly, has led to the development of u
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9

Argoud, G. M., D. S. Schade, and R. P. Eaton. "Underestimation of hepatic glucose production by radioactive and stable tracers." American Journal of Physiology-Endocrinology and Metabolism 252, no. 5 (1987): E606—E615. http://dx.doi.org/10.1152/ajpendo.1987.252.5.e606.

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Although negative hepatic glucose production rates are physiologically impossible, they have been observed when hepatic glucose production is measured with the tracer-dilution technique during the hyperinsulinemic, euglycemic glucose clamp. Because hepatic glucose production is determined from the difference between tracer-derived glucose disposal and the known exogenous glucose infusion rate, the negative values for hepatic glucose production must result from an underestimation of glucose disposal by the tracer technique. In the current investigation, tracer-derived glucose disposal was measu
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10

Jackisch, Dominik, Bi Xuan Yeo, Adam D. Switzer, et al. "Precipitation stable isotopic signatures of tropical cyclones in Metropolitan Manila, Philippines, show significant negative isotopic excursions." Natural Hazards and Earth System Sciences 22, no. 1 (2022): 213–26. http://dx.doi.org/10.5194/nhess-22-213-2022.

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Abstract. Tropical cyclones have devastating impacts on the environment, economies, and societies and may intensify in the coming decades due to climate change. Stable water isotopes serve as tracers of the hydrological cycle, as isotope fractionation processes leave distinct precipitation isotopic signatures. Here we present a record of daily precipitation isotope measurements from March 2014 to October 2015 for Metropolitan Manila, a first-of-a-kind dataset for the Philippines and Southeast Asia. We show that precipitation isotopic variation at our study site is closely related to tropical c
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11

Umpleby, A. Margot. "HORMONE MEASUREMENT GUIDELINES: Tracing lipid metabolism: the value of stable isotopes." Journal of Endocrinology 226, no. 3 (2015): G1—G10. http://dx.doi.org/10.1530/joe-14-0610.

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Labelling molecules with stable isotopes to create tracers has become a gold-standard method to study the metabolism of lipids and lipoproteins in humans. There are a range of techniques which use stable isotopes to measure fatty acid flux and oxidation, hepatic fatty synthesis, cholesterol absorption and synthesis and lipoprotein metabolism in humans. Stable isotope tracers are safe to use, enabling repeated studies to be undertaken and allowing studies to be undertaken in children and pregnant women. This review provides details of the most appropriate tracers to use, the techniques which ha
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12

Corfield, Richard M., and Richard D. Norris. "Isotope Paleobiology and Paleoecology: So Why Should Paleontologists Care About Geochemistry?" Paleontological Society Papers 4 (October 1998): 1–6. http://dx.doi.org/10.1017/s1089332600000371.

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Stable isotopic techniques in geology illuminate not only variations in past climates and oceans, but also the life-histories of extinct animals, plants and protistans. This volume focuses on the ways that stable isotopes can be used as tracers of the fossil biology and ecology of long-dead organisms and ecosystems. Here, we introduce relevant aspects of stable isotope systematics and provide a summary of the papers collected in this volume. The nine contributions collected here, from some of the most eminent workers in their respective fields, explore aspects of the ecology, evolution and bio
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13

Atherton, Philip, Matthew Brook, and Daniel Wilkinson. "Stable isotope tracers in muscle physiology research." Physiology News, Winter 2016 (January 1, 2017): 25–29. http://dx.doi.org/10.36866/pn.105.25.

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14

Bramlet, Roland. "Radioactive and Stable Isotope Tracers in Biomedicine." Clinical Nuclear Medicine 18, no. 5 (1993): 455–56. http://dx.doi.org/10.1097/00003072-199305000-00027.

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15

Knapp, Julia L. A., Colin Neal, Alessandro Schlumpf, Margaret Neal, and James W. Kirchner. "New water fractions and transit time distributions at Plynlimon, Wales, estimated from stable water isotopes in precipitation and streamflow." Hydrology and Earth System Sciences 23, no. 10 (2019): 4367–88. http://dx.doi.org/10.5194/hess-23-4367-2019.

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Abstract. Long-term, high-frequency time series of passive tracers in precipitation and streamflow are essential for quantifying catchment transport and storage processes, but few such data sets are publicly available. Here we describe, present, and make available to the public two extensive data sets of stable water isotopes in streamflow and precipitation at the Plynlimon experimental catchments in central Wales. Stable isotope data are available at 7-hourly intervals for 17 months, and at weekly intervals for 4.25 years. Precipitation isotope values were highly variable in both data sets, a
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16

Yergey, Alfred L., Steven A. Abrams, Nancy E. Vieira, Richard Eastell, Laura S. Hillman, and David G. Covell. "Recent studies of human calcium metabolism using stable isotopic tracers." Canadian Journal of Physiology and Pharmacology 68, no. 7 (1990): 973–76. http://dx.doi.org/10.1139/y90-147.

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Stable isotopes of calcium are used safely as tracers for calcium in human populations ranging in age from infants to postmenopausal women. Thermal ionization mass spectrometry is used to measure calcium isotope ratios with relative accuracies of about 1% for natural abundance ratios at precisions of about 1% relative to the mean. Perturbations of natural abundance ratios are determined for the calcium in blood, urine, and feces with a limit of detection of about 2 Δ% excess. The mathematical rationale for clinical studies of fractional absorption of dietary calcium and the kinetics of calcium
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17

Preston, Tom. "Existing and Emerging Technologies for Measuring Stable Isotope Labelled Retinol in Biological Samples: Isotope Dilution Analysis of Body Retinol Stores." International Journal for Vitamin and Nutrition Research 84, Supplement 1 (2014): 30–39. http://dx.doi.org/10.1024/0300-9831/a000186.

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This paper discusses some of the recent improvements in instrumentation used for stable isotope tracer measurements in the context of measuring retinol stores, in vivo. Tracer costs, together with concerns that larger tracer doses may perturb the parameter under study, demand that ever more sensitive mass spectrometric techniques are developed. GCMS is the most widely used technique. It has high sensitivity in terms of sample amount and uses high resolution GC, yet its ability to detect low isotope ratios is limited by background noise. LCMSMS may become more accessible for tracer studies. Its
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18

Chandra, Subhash. "Imaging transported and endogenous calcium independently at a subcellular resolution: ion microscopy imaging of calcium stable isotopes." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 2 (1992): 1604–5. http://dx.doi.org/10.1017/s0424820100132650.

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Ion microscopy, based on secondary ion mass spectrometry (SIMS), is a unique isotopic imaging technique. The use of stable isotopes as tracers and their SIMS localization at a subcellular resolution has introduced a significant new approach for molecular localization and ion transport studies. A molecule of interest may be tagged with stable 2H, 13C, 15N, etc. and imaged with SIMS for its intracellular location. Stable isotopes of physiologically important elements such as calcium and magnesium provide excellent tracers for ion transport imaging studies with SIMS. in a recent study with 44Ca,
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19

Brkić, Željka, Mladen Kuhta, Tamara Hunjak, and Ozren Larva. "Regional Isotopic Signatures of Groundwater in Croatia." Water 12, no. 7 (2020): 1983. http://dx.doi.org/10.3390/w12071983.

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Tracer methods are useful for investigating groundwater travel times and recharge rates and analysing impacts on groundwater quality. The most frequently used tracers are stable isotopes and tritium. Stable isotopes of oxygen (δ18O) and hydrogen (δ2H) are mainly used as indicators of the recharge condition. Tritium (3H) is used to estimate an approximate mean groundwater age. This paper presents the results of an analysis of stable isotope data and tritium activity in Croatian groundwater samples that were collected between 1997 and 2014 at approximately 100 sites. The composition of the stabl
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20

Jahn, A., K. Lindsay, X. Giraud, et al. "Carbon isotopes in the ocean model of the Community Earth System Model (CESM1)." Geoscientific Model Development 8, no. 8 (2015): 2419–34. http://dx.doi.org/10.5194/gmd-8-2419-2015.

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Abstract. Carbon isotopes in the ocean are frequently used as paleoclimate proxies and as present-day geochemical ocean tracers. In order to allow a more direct comparison of climate model results with this large and currently underutilized data set, we added a carbon isotope module to the ocean model of the Community Earth System Model (CESM), containing the cycling of the stable isotope 13C and the radioactive isotope 14C. We implemented the 14C tracer in two ways: in the "abiotic" case, the 14C tracer is only subject to air–sea gas exchange, physical transport, and radioactive decay, while
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Jahn, A., K. Lindsay, X. Giraud, et al. "Carbon isotopes in the ocean model of the Community Earth System Model (CESM1)." Geoscientific Model Development Discussions 7, no. 6 (2014): 7461–503. http://dx.doi.org/10.5194/gmdd-7-7461-2014.

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Abstract. Carbon isotopes in the ocean are frequently used as paleo climate proxies and as present-day geochemical ocean tracers. In order to allow a more direct comparison of climate model results with this large and currently underutilized dataset, we added a carbon isotope module to the ocean model of the Community Earth System Model (CESM), containing the cycling of the stable isotope 13C and the radioactive isotope 14C. We implemented the 14C tracer in two ways: in the "abiotic" case, the 14C tracer is only subject to air–sea gas exchange, physical transport, and radioactive decay, while
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Middelburg, J. J. "Stable isotopes dissect food webs from top to the bottom." Biogeosciences Discussions 10, no. 9 (2013): 14923–52. http://dx.doi.org/10.5194/bgd-10-14923-2013.

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Abstract. Stable isotopes have been used extensively to study food web functioning, i.e. the flow of energy and matter among organisms. Traditional food-web studies are based on the natural variability of carbon and nitrogen isotopes and are limited to larger organisms that can be physically separated from their environment. Recent developments allow isotope ratio measurements of microbes and this in turn allows then measurement of entire food webs, i.e. from small producers at the bottom to large consumers at the top. Here, I provide a concise review on the use and potential of stable isotope
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Vogt, J. "Availability of stable isotope tracers for human use." European Journal of Pediatrics 156, S1 (1997): S9—S11. http://dx.doi.org/10.1007/pl00014281.

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24

Ala-aho, Pertti, Doerthe Tetzlaff, James P. McNamara, Hjalmar Laudon, and Chris Soulsby. "Using isotopes to constrain water flux and age estimates in snow-influenced catchments using the STARR (Spatially distributed Tracer-Aided Rainfall–Runoff) model." Hydrology and Earth System Sciences 21, no. 10 (2017): 5089–110. http://dx.doi.org/10.5194/hess-21-5089-2017.

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Abstract. Tracer-aided hydrological models are increasingly used to reveal fundamentals of runoff generation processes and water travel times in catchments. Modelling studies integrating stable water isotopes as tracers are mostly based in temperate and warm climates, leaving catchments with strong snow influences underrepresented in the literature. Such catchments are challenging, as the isotopic tracer signals in water entering the catchments as snowmelt are typically distorted from incoming precipitation due to fractionation processes in seasonal snowpack. We used the Spatially distributed
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Leketa, Khahliso, and Tamiru Abiye. "Using Environmental Tracers to Characterize Groundwater Flow Mechanisms in the Fractured Crystalline and Karst Aquifers in Upper Crocodile River Basin, Johannesburg, South Africa." Hydrology 8, no. 1 (2021): 50. http://dx.doi.org/10.3390/hydrology8010050.

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Environmental isotope tracers were applied in the Upper Crocodile River Basin, Johannesburg, South Africa, to understand the groundwater recharge conditions, flow mechanisms and interactions between surface and subsurface water. Stable isotope analysis indicated that recharge into the fractured quartzite aquifer occurs through direct mechanisms. The high variability in the stable isotope signature of temporal samples from Albert Farm spring indicated the importance of multiple samples for groundwater characterization, and that using a single sample may be yielding biased conclusions. The obser
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Doucet, Luc S., Oscar Laurent, Dmitri A. Ionov, Nadine Mattielli, Vinciane Debaille, and Wendy Debouge. "Archean lithospheric differentiation: Insights from Fe and Zn isotopes." Geology 48, no. 10 (2020): 1028–32. http://dx.doi.org/10.1130/g47647.1.

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Abstract The Archean continental lithosphere consists of a dominantly felsic continental crust, made of tonalite-trondhjemite-granodiorite (TTG) and subordinate granitoids, and a cratonic lithospheric mantle, made of highly refractory peridotites. Whether they stemmed from the same process of differentiation from the primitive mantle, or were two distinct components that were physically juxtaposed, remains debated. Metal stable isotope ratios are sensitive to magmatic and metamorphic processes and do not evolve with time. Therefore, stable isotope ratios are complementary to radiogenic isotope
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27

Rosenblatt, J., D. Chinkes, M. Wolfe, and R. R. Wolfe. "Stable isotope tracer analysis by GC-MS, including quantification of isotopomer effects." American Journal of Physiology-Endocrinology and Metabolism 263, no. 3 (1992): E584—E596. http://dx.doi.org/10.1152/ajpendo.1992.263.3.e584.

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In metabolic tracer studies it is frequently useful to infuse tracers that are differently labeled variants of the same molecule. These tracers are known as isotopomers. Analysis of the enrichment of each isotopic analogue can be accomplished by gas chromatography-mass spectrometry (GC-MS). However, the raw GC-MS data must be corrected to give the information required. This paper addresses how to transform the raw GC-MS data, consisting of relative abundance ratios at specific ion masses, into relative molar ratios of tracer and tracee molecules. Several correction factors are necessary. First
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Miljević, Nada, and Dušan Golobočanin. "Potential Use of Environmental Isotopes in Pollutant Migration Studies." Archives of Industrial Hygiene and Toxicology 58, no. 2 (2007): 251–62. http://dx.doi.org/10.2478/v10004-007-0015-5.

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Potential Use of Environmental Isotopes in Pollutant Migration StudiesThis article presents the use of natural abundance stable isotope (hydrogen, carbon, nitrogen, oxygen, chlorine) analysis data as a tool for providing important information about the origin of contaminants, the contribution of different sources to a multi-source plume, characterisation of their complex transport (rate and mechanisms) and for evaluating the success of contaminated site remediation. Isotopic signatures of contaminants are useful tracers of their sources, while isotopic fractionation can be used to quantitative
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29

Middelburg, J. J. "Stable isotopes dissect aquatic food webs from the top to the bottom." Biogeosciences 11, no. 8 (2014): 2357–71. http://dx.doi.org/10.5194/bg-11-2357-2014.

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Abstract. Stable isotopes have been used extensively to study food-web functioning, that is, the flow of energy and matter among organisms. Traditional food-web studies are based on the natural variability of isotopes and are limited to larger organisms that can be physically separated from their environment. Recent developments allow isotope ratio measurements of microbes and this in turn allows the measurement of entire food webs, in other words, from small producers at the bottom to large consumers at the top. Here, I provide a concise review on the use and potential of stable isotopes to r
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Wang, Pei, Yujing Deng, and Zhongwang Wei. "Modeling Investigation of Diurnal Variations in Water Flux and Its Components with Stable Isotopic Tracers." Atmosphere 10, no. 7 (2019): 403. http://dx.doi.org/10.3390/atmos10070403.

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The isotopic compositions of water fluxes provide valuable insights into the hydrological cycle and are widely used to quantify biosphere–atmosphere exchange processes. However, the combination of water isotope approaches with water flux components remains challenging. The Iso-SPAC (coupled heat, water with isotopic tracer in soil–plant–atmosphere-continuum) model is a useful framework for simulating the dynamics of water flux and its components, and for coupling with isotopic fractionation and mixing processes. Here, we traced the isotopic fractionation processes with separate soil evaporatio
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Young, Vernon R., and Alfred Ajami. "Isotopes in nutrition research." Proceedings of the Nutrition Society 58, no. 1 (1999): 15–32. http://dx.doi.org/10.1079/pns19990004.

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The present lecture begins with a brief overview of the professional and scientific journey taken by Rudolf Schoenheimer, before turning to a discussion of the power of isotopic tracers in nutrition research. Schoenheimer's remarkable contributions to the study of intermediary metabolism and the turnover of body constituents, based initially on compounds tagged with2H and later with15N, spanned a mere decade. It is difficult, however, to overestimate the enormous impact of Schoenheimer's research on the evolution of biological science. After a relative hiatus, following Schoenheimer's death in
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von Freyberg, Jana, Bjørn Studer, and James W. Kirchner. "A lab in the field: high-frequency analysis of water quality and stable isotopes in stream water and precipitation." Hydrology and Earth System Sciences 21, no. 3 (2017): 1721–39. http://dx.doi.org/10.5194/hess-21-1721-2017.

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Abstract. High-frequency measurements of solutes and isotopes (18O and 2H) in rainfall and streamflow can shed important light on catchment flow pathways and travel times, but the workload and sample storage artifacts involved in collecting, transporting, and analyzing thousands of bottled samples severely constrain catchment studies in which conventional sampling methods are employed. However, recent developments towards more compact and robust analyzers have now made it possible to measure chemistry and water isotopes in the field at sub-hourly frequencies over extended periods. Here, we pre
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Li, Jing, Fan Song, Zhicheng Bao, Hongxiang Fan, and Huawu Wu. "Insights into Shallow Freshwater Lakes Hydrology in the Yangtze Floodplain from Stable Water Isotope Tracers." Water 14, no. 3 (2022): 506. http://dx.doi.org/10.3390/w14030506.

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Stable isotopes of lake waters are widely used to identify the relative importance of hydrological processes on the lake water balance across the ungauged landscape via the coupled-isotope tracer model. The isotopic compositions of twenty shallow freshwater lakes across the mid-lower reaches of Yangtze floodplain (MLY) were investigated in January and May of 2018. The lake-specific input water (δI) and evaporation-to-inflow (E/I) ratios were estimated to explore the specific lake hydrology across the MLY. Results showed that distinct isotopic enrichment trends in May compared with those in Jan
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Lane, Andrew N., Teresa W. M. Fan, Richard M. Higashi, Jinlian Tan, Michael Bousamra, and Donald M. Miller. "Prospects for clinical cancer metabolomics using stable isotope tracers." Experimental and Molecular Pathology 86, no. 3 (2009): 165–73. http://dx.doi.org/10.1016/j.yexmp.2009.01.005.

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35

Wiederhold, Jan G. "Metal Stable Isotope Signatures as Tracers in Environmental Geochemistry." Environmental Science & Technology 49, no. 5 (2015): 2606–24. http://dx.doi.org/10.1021/es504683e.

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36

Abart, R. "Geometry of stable isotope fronts: Multiple monitors and tracers." Chinese Science Bulletin 43, S1 (1998): 1. http://dx.doi.org/10.1007/bf02891349.

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Ganot, Yonatan, Ran Holtzman, Noam Weisbrod, et al. "Managed aquifer recharge with reverse-osmosis desalinated seawater: modeling the spreading in groundwater using stable water isotopes." Hydrology and Earth System Sciences 22, no. 12 (2018): 6323–33. http://dx.doi.org/10.5194/hess-22-6323-2018.

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Abstract. The spreading of reverse-osmosis desalinated seawater (DSW) in the Israeli coastal aquifer was studied using groundwater modeling and stable water isotopes as tracers. The DSW produced at the Hadera seawater reverse-osmosis (SWRO) desalination plant is recharged into the aquifer through an infiltration pond at the managed aquifer recharge (MAR) site of Menashe, Israel. The distinct difference in isotope composition between DSW (δ18O = 1.41 ‰; δ2H = 11.34 ‰) and the natural groundwater (δ18O = −4.48 ‰ to −5.43 ‰; δ2H = −18.41 ‰ to −22.68 ‰) makes the water isotopes preferable for use
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38

Wastney, M. E., I. G. Gokmen, R. L. Aamodt, W. F. Rumble, G. E. Gordon, and R. I. Henkin. "Kinetic analysis of zinc metabolism in humans after simultaneous administration of 65Zn and 70Zn." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 260, no. 1 (1991): R134—R141. http://dx.doi.org/10.1152/ajpregu.1991.260.1.r134.

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Zinc kinetics were studied and compared after oral simultaneous administration of two tracers, radioactive (65Zn) and stable (70Zn) isotope, to four normal human volunteers. Both tracers and zinc concentration were measured in plasma, red blood cells (RBC), urine, and feces for up to 78 days. Radioactive zinc was also measured by external counting over whole body, liver, and thigh. Data from each individual were analyzed using a compartmental model for zinc metabolism. Values calculated for absorption, fractional zinc excretion in urine, exchange with RBC, and secretion into gut using 70Zn dat
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Lin, Penghui, Li Dai, Daniel R. Crooks, et al. "NMR Methods for Determining Lipid Turnover via Stable Isotope Resolved Metabolomics." Metabolites 11, no. 4 (2021): 202. http://dx.doi.org/10.3390/metabo11040202.

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Lipids comprise diverse classes of compounds that are important for the structure and properties of membranes, as high-energy fuel sources and as signaling molecules. Therefore, the turnover rates of these varied classes of lipids are fundamental to cellular function. However, their enormous chemical diversity and dynamic range in cells makes detailed analysis very complex. Furthermore, although stable isotope tracers enable the determination of synthesis and degradation of complex lipids, the numbers of distinguishable molecules increase enormously, which exacerbates the problem. Although LC-
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Guo, Zengkui, and Michael D. Jensen. "Intramuscular fatty acid metabolism evaluated with stable isotopic tracers." Journal of Applied Physiology 84, no. 5 (1998): 1674–79. http://dx.doi.org/10.1152/jappl.1998.84.5.1674.

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We evaluated the applicability of stable isotopic tracers to the study of intramuscular fatty acid metabolism by infusing both [U-13C]palmitate and [1-13C]oleate intravenously for 4 h into fasted conscious rats. Skeletal muscles were sequentially biopsied, and the concentration and13C enrichment of fatty acids were measured by gas chromatography/combustion/isotope ratio mass spectrometry. Throughout the study, the13C enrichment of plasma palmitate and oleate remained substantially greater than intramuscular nonesterified palmitate and oleate enrichment, which in turn was greater than intramusc
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Andrei, Francesca, Maurizio Barbieri, and Giuseppe Sappa. "Application of 2H and 18O Isotopes for Tracing Municipal Solid Waste Landfill Contamination of Groundwater: Two Italian Case Histories." Water 13, no. 8 (2021): 1065. http://dx.doi.org/10.3390/w13081065.

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Groundwater contamination due to municipal solid waste landfills leachate is a serious environmental threat. During recent years, the use of stable isotopes as environmental tracers to identify groundwater contamination phenomena has found application to environmental engineering. Deuterium (2H) and oxygen (18O) isotopes have successfully used to identify groundwater contamination phenomena if submitted to interactions with municipal solid waste landfills leachate, with a significant organic amount. The paper shows two case studies, in central and southern Italy, where potential contamination
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Chen, Jie-Hua, Nadia Singh, Huimin Tay, and Thomas Walczyk. "Imbalance of iron influx and efflux causes brain iron accumulation over time in the healthy adult rat." Metallomics 6, no. 8 (2014): 1417–26. http://dx.doi.org/10.1039/c4mt00054d.

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Tuvdendorj, Demidmaa, David L. Chinkes, David N. Herndon, Xiao-Jun Zhang, and Robert R. Wolfe. "A novel stable isotope tracer method to measure muscle protein fractional breakdown rate during a physiological non-steady-state condition." American Journal of Physiology-Endocrinology and Metabolism 304, no. 6 (2013): E623—E630. http://dx.doi.org/10.1152/ajpendo.00552.2012.

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The measurement of the fractional breakdown rate (FBR) of muscle proteins during physiological non-steady state of amino acids (AAs) presents some challenges. Therefore, the goal of the present experiment was to modify the bolus stable isotope tracer injection approach to determine both fractional synthesis rate (FSR) and FBR of leg muscle protein during a physiological non-steady state of AAs. The approach uses the traditional precursor-product principle but is modified with the assumption that inward transport of AAs is proportional to their plasma concentrations. The FBR value calculated fr
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Wilkinson, Daniel J., Matthew S. Brook, Kenneth Smith, and Philip J. Atherton. "Stable isotope tracers and exercise physiology: past, present and future." Journal of Physiology 595, no. 9 (2016): 2873–82. http://dx.doi.org/10.1113/jp272277.

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O'Brien, Kimberly O., and Steven A. Abrams. "Using stable isotope tracers to study bone metabolism in children." Journal of Physiology 597, no. 5 (2018): 1311–19. http://dx.doi.org/10.1113/jp275452.

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Preston, Tom, and Christine Slater. "Mass spectrometric analysis of stable-isotope-labelled amino acid tracers." Proceedings of the Nutrition Society 53, no. 2 (1994): 363–72. http://dx.doi.org/10.1079/pns19940042.

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Williams, Alan E. "Stable isotope tracers: natural and anthropogenic recharge, Orange County, California." Journal of Hydrology 201, no. 1-4 (1997): 230–48. http://dx.doi.org/10.1016/s0022-1694(97)00042-5.

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Nijenhuis, Ivonne, Nicole Stelzer, Matthias Kästner, and Hans-H. Richnow. "Sensitive Detection of Anaerobic Monochlorobenzene Degradation Using Stable Isotope Tracers." Environmental Science & Technology 41, no. 11 (2007): 3836–42. http://dx.doi.org/10.1021/es0621896.

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Kim, Il-Young, Sanghee Park, Jiwoong Jang, Yeongmin Kim, and Hee-Joo Kim. "Exploring Human Muscle Dynamics In Vivo Using Stable Isotope Tracers." Annals of Clinical Nutrition and Metabolism 14, no. 2 (2022): 40–49. http://dx.doi.org/10.15747/acnm.2022.14.2.40.

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Watson, Elizabeth Burke, Alejandro Hinojosa-Corona, Johannes R. Krause, et al. "Lagoon Biogeochemical Processing is Reflected in Spatial Patterns of Sediment Stable Isotopic Ratios." Journal of Marine Science and Engineering 8, no. 11 (2020): 874. http://dx.doi.org/10.3390/jmse8110874.

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The spatial analysis of biota, particulate organic matter, and sediments for stable isotopes of carbon (δ13C), nitrogen (δ15N), and sulfur (δ34S) have proved useful for identifying patterns in productivity, nutrient pollution, and relationships between biological and physiochemical variables at the local and global scales. Yet such approaches are rarely applied to studies of lagoon or estuarine metabolism. Focusing on Bahía San Quintín, a heterotrophic seagrass-dominated lagoon on the Pacific coast of Baja California, México, we report on spatial patterns in surficial sediment CNS stable isoto
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