Relevant bibliographies by topics / Stable isotope dilution assay

Academic literature on the topic 'Stable isotope dilution assay'

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Published: 4 June 2021
Last updated: 4 February 2022

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Journal articles on the topic "Stable isotope dilution assay"

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Asam, Stefan, Yang Liu, Katharina Konitzer, and Michael Rychlik. "Development of a Stable Isotope Dilution Assay for Tenuazonic Acid." Journal of Agricultural and Food Chemistry 59, no. 7 (April 13, 2011): 2980–87. http://dx.doi.org/10.1021/jf104270e.

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Rychlik, Michael, and Stefan Asam. "Stable isotope dilution assays in mycotoxin analysis." Analytical and Bioanalytical Chemistry 390, no. 2 (December 1, 2007): 617–28. http://dx.doi.org/10.1007/s00216-007-1717-x.

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Büttner, Barbara E., Veronica E. Öhrvik, Peter Köhler, Cornelia M. Witthöft, and Michael Rychlik. "Quantification of Isotope-Labeled and Unlabeled Folates and Folate Catabolites in Urine Samples by Stable Isotope Dilution Assay." International Journal for Vitamin and Nutrition Research 83, no. 2 (April 1, 2013): 112–21. http://dx.doi.org/10.1024/0300-9831/a000152.

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Dual-label stable isotope dilution assays for the simultaneous quantification of isotopologic folates in clinical samples offer the perspective for differentiating between unlabeled folates from endogenous body pools and administered [13C5]-labeled folates from a test dose when performing bioavailability trials. In contrast to intact folates, this methodology could hitherto not be applied to the quantification of the folate catabolites, p-aminobenzoyl glutamate and p-acetamidobenzoyl glutamate. In this study, [2H4]-p-aminobenzoyl glutamate, [2H4]-p-acetamidobenzoyl glutamate, and unlabeled p-acetamidobenzoyl glutamate were synthesized. The synthesis of the [2H4]-labeled compounds started at unlabeled p-aminobenzoic acid. For the formation of p-acetamidobenzoyl glutamate, p-aminobenzoyl glutamate was acetylated. The new substances were applied successfully in stable isotope dilution assays for the simultaneous quantification of the [13C5]-labeled and unlabeled folate catabolites, p-aminobenzoyl glutamate and p-acetamidobenzoyl glutamate, along with the predominant folate vitamers in urine. The assays were based on clean-up by strong anion exchange followed by liquid chromatography-tandem mass spectrometry detection. Assay sensitivity was sufficient to detect the folate catabolites in physiologic concentrations. The limit of detection was below 0.4 and 0.3 nmol/100 g for p-aminobenzoyl glutamate isotopologues and p-acetamidobenzoyl glutamate isotopologues in urine, respectively. The successful synthesis of [2H4]-p-aminobenzoyl glutamate, [2H4]-p-acetamidobenzoyl glutamate, and unlabeled p-acetamidobenzoyl glutamate and the implementation of these substances in stable isotope dilution assays allows dual-label designs that provide a more detailed insight into human folate metabolism.
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Dufour, Jean-Pierre, Rana Wierda, Michelle Leus, Geert Lissens, Freddy Delvaux, Guy Derdelinckx, and David Larsen. "Quantitative Analysis of Beer Aromatic Alcohols Using Stable Isotope Dilution Assay." Journal of the American Society of Brewing Chemists 60, no. 2 (April 2002): 88–96. http://dx.doi.org/10.1094/asbcj-60-0088.

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Rychlik, Michael, and Peter Schieberle. "Quantification of the Mycotoxin Patulin by a Stable Isotope Dilution Assay." Journal of Agricultural and Food Chemistry 47, no. 9 (September 1999): 3749–55. http://dx.doi.org/10.1021/jf990198a.

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Shinohara, Y., Y. Suzuki, H. Hasegawa, M. Nakamura, T. Nishiyama, A. Hiratsuka, and K. Ichida. "Stable Isotope Dilution Mass Spectrometric Assay for PRPP Using Enzymatic Procedures." Nucleosides, Nucleotides and Nucleic Acids 30, no. 12 (December 2011): 1140–46. http://dx.doi.org/10.1080/15257770.2011.591746.

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Zeller, Annette, and Michael Rychlik. "Quantitation of estragole by stable isotope dilution assays." LWT - Food Science and Technology 42, no. 3 (April 2009): 717–22. http://dx.doi.org/10.1016/j.lwt.2008.10.011.

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Rychlik and Roth-Maier. "Pantothenic Acid Quantification: Method Comparison of a Stable Isotope Dilution Assay and a Microbiological Assay." International Journal for Vitamin and Nutrition Research 75, no. 3 (May 1, 2005): 218–23. http://dx.doi.org/10.1024/0300-9831.75.3.218.

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Different foods and feedstuffs were analyzed for pantothenic acid (PA) by the recently developed stable isotope dilution assay (SIDA) and by the standard method, a microbiological assay (MA). The SIDA involved the use of [13C3, 15N]-pantothenic acid as the internal standard and detection by liquid chromatography-tandem mass spectrometry. The analysis of identical extracts minimized systematic bias due to equal extraction yields and enabled an ideal comparison between both methods. For the samples derived from plants a good accordance between the MA and the SIDA of total PA was found, whereas for the products of animal origin, higher contents were measured by MA than by SIDA. From the results of treatments by pantetheinase and phosphatase on the one hand and papain and diastase on the other, it was concluded that MA is able to measure a significant amount of bound PA. Furthermore, the data imply that microbial enzymes were able to cleave PA conjugates more effectively than pantetheinase and phosphatase treatment.
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Sen, Alina, Gudrun Laskawy, Peter Schieberle, and Werner Grosch. "Quantitative determination of .beta.-damascenone in foods using a stable isotope dilution assay." Journal of Agricultural and Food Chemistry 39, no. 4 (April 1991): 757–59. http://dx.doi.org/10.1021/jf00004a028.

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Dollmann, Bettina, Dominicus Wichmann, Alfred Schmitt, Hans Koehler, and Peter Schreier. "Quantitative Analysis of 2-Aminoacetophenone in Off-Flavored Wines by Stable Isotope Dilution Assay." Journal of AOAC INTERNATIONAL 79, no. 2 (March 1, 1996): 583–86. http://dx.doi.org/10.1093/jaoac/79.2.583.

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Abstract Isotope dilution analysis was used to quantitate 2- aminoacetophenone in wines exhibiting the so- called untypical aging off-flavor. d3-Aminoacetophe- none was synthesized and used as isotopomeric internal standard. The method of quantitation was verified by several model experiments. In the off-flavored wines studied, amounts of 2-aminoacetophe none ranging from 0.7 to 12.8 μg/L were determined.
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Dissertations / Theses on the topic "Stable isotope dilution assay"

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Brown, Rachel Christine, and rcbrown@adam com au. "gamma-Lactones in wine: Synthesis, quantification and sensory studies." Flinders University. School of Chemistry, Physics and Earth Sciences, 2007. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20080226.234630.

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gamma-Lactones are found in a wide variety of food and beverage products, in particular grapes and wine. This thesis details the work completed on some gamma-lactones in wine: their synthetic preparation, development of quantification methodologies and sensory studies. Chapter 1 outlines the history of the Australian wine industry from the arrival of the first vines on the First Fleet in 1788 with Captain Arthur Philip. This chapter provides: an overview of Australia’s position in the world of grape and wine production; an analysis of the export arm of the industry; and a look at the different wine producing regions around the country. The latter part of the chapter focuses on the different volatile compounds found in wine. Part A: Chapter 2 provides an overview on the history of barrel manufacture and the use of oak wood in cooperage, with an emphasis on oak’s well known ability to impart desirable characteristics to wine through the extraction of volatile aroma compounds. This chapter provides a summary of these odorants with a particular emphasis on the oak lactones. Previous sensory studies and synthetic work are discussed. Of great importance to this work are the recent advancements in 1,2-dioxine chemistry, highlighted in this chapter. Chapter 3 details the synthetic work completed for the preparation of all four possible oak lactone stereoisomers. A suitably substituted racemic 1,2-dioxine featured as the common intermediate and enabled preparation of the gamma-lactone moiety upon reaction with a chiral malonate diester and separation of the diastereomers by column chromatography. A key step involved the decarboxylation of the ester cleaved gamma-lactone diastereomers, which could be directed to give either the cis- or trans-products. Standard chemical transformations were then utilised to produce the desired stereoisomers of oak lactone. Chapter 4 describes the results from the sensory studies that were completed on the synthetic oak lactone samples. Odour detection thresholds were measured in both a white and a red wine. The thresholds in the former medium were calculated to be 24 ug/L, 172 ug/L, 132 ug/L and 305 ug/L, while in the latter medium the thresholds were calculated to be 57 ug/L, 380 ug/L, 175 ug/L and 285 ug/L, for (4S,5S)-cis-, (4S,5R)-trans-, (4R,5R)-cis- and (4R,5S)-trans-oak lactone, respectively. Difference testings were completed on the pairs of enantiomers and also on mixtures of the nature-identical isomers: between the cis-enantiomers a significant difference was found at the 99% confidence level, while between the trans-enantiomers and also the mixtures of cis- and trans-isomers little difference was observed. Chapter 5 contains the experimental procedures for Part A. Part B: Chapter 6 discusses the sensory properties of some gamma- and delta-lactones, with the focus on a series of five-alkyl substituted gamma-lactones: gamma-octalactone, gamma-nonalactone, gamma-decalactone and gamma-dodecalactone. Topics covered in this chapter include chirality, biosynthetic pathways and quantification results in wine from previous studies for these gamma-lactones. Chapter 7 concerns the method development for the quantification of gamma-lactones in wine using a stable isotope dilution assay (SIDA). Deuterated analogues were prepared from commercially available racemic gamma-lactones for use as internal standards. Initially a head space solid-phase microextraction (HS SPME) method was developed using d5-standards; however, analysis of bottled wine samples revealed the presence of co-eluting compounds that contained several of the selected ions. Thus an alternative method was developed using d7-standards, with a specific focus on sample clean-up, via solid-phase extraction (SPE). Using this procedure, 44 white and 120 red wines were analysed for their gamma-lactone content. The lactones were found to be significantly more common in the red wines, with gamma-nonalactone the most abundant lactone in this series. Chapter 8 deals with the extension of the SIDA method, as developed in Chapter 7, for use with a chiral gas chromatography column. Optically pure standards were prepared, from either L- or D-glutamic acid, and used to determine the order of elution of the enantiomers. A method was developed for the quantification of the individual enantiomers of gamma-octalactone, gamma-nonalactone, gamma-decalactone and gamma-dodecalactone. The enantiomeric distribution of gamma-nonalactone was investigated in 34 red wines; the (R)-stereoisomer was found to be dominant with an average of 59%, although there were wines analysed that did contain the (S)-stereoisomer in greater amounts. Chapter 9 describes the results from the sensory studies that were completed on the individual enantiomers of the gamma-lactones. Odour detection thresholds were measured in a red wine. The thresholds were calculated to be 238 ug/L, 285 ug/L, 34 ug/L and 8 ug/L for the (R)-enantiomers, while the thresholds were calculated to be 135 ug/L, 91 ug/L, 47 ug/L and 39 ug/L for the (S)-enantiomers, of gamma-octalactone, gamma-nonalactone, gamma-decalactone and gamma-dodecalactone, respectively. Chapter 10 contains the experimental procedures for Part B. Chapter 11 contains the appendices, followed by the references in Chapter 12.
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Zech, Julie [Verfasser], Leif-Alexander [Akademischer Betreuer] Garbe, Hajo [Gutachter] Haase, Juri [Gutachter] Rappsilber, and Sascha [Gutachter] Rohn. "Analysis of bisphenols and bisphenol A diglycidyl ethers by stable isotope dilution assay liquid chromatography-tandem mass spectrometry / Julie Zech ; Gutachter: Hajo Haase, Juri Rappsilber, Sascha Rohn ; Betreuer: Leif-Alexander Garbe." Berlin : Technische Universität Berlin, 2016. http://d-nb.info/1156011728/34.

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Hu, Ling [Verfasser], Michael [Akademischer Betreuer] Rychlik, and Peter [Akademischer Betreuer] Köhler. "Development and Application of Stable Isotope Dilution Assays for the Fusarium Mycotoxins Enniatins and Beauvericin / Ling Hu. Gutachter: Peter Köhler ; Michael Rychlik. Betreuer: Michael Rychlik." München : Universitätsbibliothek der TU München, 2014. http://d-nb.info/1047185504/34.

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Marzouk, Ezzat Rashad El-Said. "Using multi-element stable isotope dilution to quantify metal reactivity in soil." Thesis, University of Nottingham, 2012. http://eprints.nottingham.ac.uk/28914/.

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Determining the total concentration of elements in soils seldom provides sufficient insight into trace metal bioavailability. However, measurement of ‘isotopically exchangeable’ metal can provide a better evaluation of metal reactivity and potential toxicity. Traditionally this requires the use of problematic radio-isotopes (e.g. 109Cd (γ)). Fortunately, increasing access to Inductively Coupled Plasma Mass Spectrometry (ICP-MS) in recent years has led to greater use of enriched stable isotopes of trace metals. The lability of heavy metals has been determined through a variety of approaches, including single and sequential extraction or predicted by geochemical models. In the present work, multi-element stable isotopes methods were developed for simultaneously determination of the labile pool of Fe, Zn, Cd and Pb using isotopic exchange principles. This included experimental and instrumental development for an accurate and precise determination of labile metal pool in soils. This approach was then validated by quantifying Zn, Cd and Pb in contaminated soils (Derbyshire; n = 8 and Weardale catchment; n = 246) and comparing the outcome results with common traditional extraction procedures. The variation of metal lability with soil characteristics was used to predict metal lability from the simple soil measurements using a multiple regression approach. In addition, E-values of Fe, Zn, Cd and Pb was used as input to WHAM(VI) (Windermere Humic-Aqueous Model) to predict metal solubility, emphasising in the role of Fe under reducing conditions in this regard. The results showed that isotopic dilution is a robust mechanistic method for assessing the ‘reactive’ pool of multiple trace metals over a wide range of soil characteristics. The results showed a very wide range of metal reactivities (almost 1%-100%) for Zn, Cd and Pb that were consistent over a range of spike concentrations. Sub-micron forms of non labile metal are perhaps most likely to occur in suspension either strongly bonded to humic/fulvic acids or occluded within CaCO3 particles. It appears that E values have no consistent correspondence to any chemical extraction procedure. Nevertheless, the use of 0.43 M HNO3 to extract labile metal in organic soils at pH < 6 appears justifiable - especially where humus is likely to be the principal adsorption surface. It is also important to acknowledge that extractions are not necessarily intended to estimate the entire reactive fraction. Thus, DTPA has been successfully applied as an empirical prediction of plant uptake but its extraction capacity is particularly limited in calcareous systems where it substantially underestimates the isotopically exchangeable metal pool. Speciation calculations showed that prediction of metal solubility was much better when the isotopically reactive metal pools were used as input to WHAM(VI). The soil samples that fitted best had pH values less than 4.0 and high organic matter contents reflecting the strength of the humic binding component of WHAM(VI) particularly in the case of Zn. The changes in metal solubility and lability under reducing conditions were mainly affected by pH. Moreover, the measurement of Fe2+ in the solution phase was considerably lower than that of the isotopically labile Fe2+ which calls into question the dependence on soluble Fe2+ to predict reductive dissolution of Fe-oxides. In addition, under reducing conditions the variables input of Fe to WHAM(VI) showed greatest effects on predicting metal solubility. It was found that Zn and Cd were affected only by Fe2+ competition for adsorption sites while predicted Pb solubility was more affected by loss of oxides than competition processes. The fractionation results, output from WHAM(VI), showed that a significant proportion of Pb was associated with Mn-oxides. Therefore, the calculation of loss of the adsorption site of Mn-oxides depending on Mn2+ measured in the solution phase did not improve the predicted Pb solubility where the model underestimate the adsorbed labile Mn as inference from Fe results.
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Lambertsson, Lars. "Mercury species transformations in marine and biological systems studied by isotope dilution mass spectrometry and stable isotope tracers." Doctoral thesis, Umeå : Univ, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-467.

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Kelly, Robert Noel. "Towards the absolute quantification of protein isoforms through the use of stable-isotope dilution mass spectrometry." Thesis, University of Glasgow, 2013. http://theses.gla.ac.uk/4401/.

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While the existence of protein was first described by Berzelius and Mulder back in 1838 and a single empirical formula noted (C400H620N100O120P1S1) (Vickery, 1950, Brand, 1946), early protein-based research was limited to the analysis of proteins which could be easily purified in large quantities, such as those obtained from blood, egg whites and those obtainable from slaughterhouses, such as digestive and metabolic enzymes (Chapman, 2005). Indeed, despite the development of recombinant deoxyribonucleic acid technologies in the 1970s (enabling protein expression) and the increasing sensitivity of techniques which enable the identification and sequencing of proteins separated by gel electrophoresis (Patterson and Aebersold, 2003), it was not until the late 1980s, with the description of soft biomolecule ionisation that large scale proteomic analyses were undertaken, based upon the use of mass spectrometry (Guerrera and Kleiner, 2005). While early mass spectrometry-based proteomic analyses focussed on the systematic identification of a great number of proteins within a single organism, the field of proteomics is now becoming increasingly quantitative (Baak et al., 2005), enabling the relative comparison of protein expression patterns between phenotypes, but also the targeted absolute quantification of specific proteins. During this project, a stable isotopically labelled internal standard based absolute quantitative technique, first described by Gerber and co-workers in 2003 (S. A. Gerber et al., 2003), was applied to the absolute quantification of three families of multiple protein isoforms. This area of research is of particular scientific interest as it is thought that up to 95% of human multi-exon genes may be subject to alternative splicing, making alternative splicing the rule, not the exception (Pan et al., 2008a). Indeed alternative splicing has also been implicated as both a cause and a consequence of disease. This technique should therefore enable both the confirmation of disease, based upon the identification of a set of phenotype specific protein biomarkers, but also the mapping of a disease’s progression (Venables, 2004). During this study, stable isotopically labelled internal standard peptides were selected for the absolute quantification of 11 confirmed protein isoforms, and two predicted protein isoforms. In addition, a separate MRM based LC-MS acquisition method was developed for the absolute quantification of each of the three families of protein isoforms (A-Raf, PDE4B and SERCA2) within a single analysis, and finally, these acquisition methods were applied to the absolute quantification of a range of immunoprecipitated, exogenously expressed protein isoforms. This project was, however, hindered by the sensitivity of the mass spectrometers available for use, preventing these acquisition methods from being applied to the absolute quantification of the endogenous levels of protein expression. While beyond the scope of this project, the further development of this quantitative technique should enable future researchers to: (i) Quantify each endogenously expressed protein isoform within a family of multiple protein isoforms. (ii) Assess any changes in the expression of each isoform in a range of cellular states, and (iii) Assess how a targeted drug treatment may affect the expression ratio of these protein isoforms.
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Lindner, Johanna Magdalena [Verfasser], and Michael [Akademischer Betreuer] Vogeser. "Novel approaches to corticosteroid profiling by stable isotope dilution tandem mass spectrometry / Johanna Magdalena Lindner ; Betreuer: Michael Vogeser." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2018. http://d-nb.info/1174142774/34.

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Vaiglova, Petra. "Neolithic agricultural management in the Eastern Mediterranean : new insight from a multi-isotope approach." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:c8824136-da35-43b2-a700-f458d0cc2fdf.

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The work presented in this dissertation explores the nature of agro-pastoral strategies developed by Neolithic farmers as a way to understand how early food production was inter-twined with environmental and socio-economic opportunities and constraints. Towards this end, a multi-isotope approach is used to address questions of scale and intensity of crop cultivation and animal management at the archaeological sites of Kouphovouno, southern Greece, Makriyalos, northern Greece, and Çatalhöyük, south-central Turkey. Measurements of stable carbon, nitrogen, oxygen and strontium isotope values of carbonized plant remains, human and animal bone collagen and animal tooth enamel are used to examine the similarities and differences in the types of treatments that individual species of plants and animals received during the agricultural cycle at the distinct locations. The results show that farmers at the three sites developed variable methods for exploiting the arable and pastoral landscape and catering to their economic and culinary needs. The discussion considers the implications of these findings to our understanding of the complexity and adaptability of early farming systems.
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Schuster, Carina [Verfasser], and Michael [Akademischer Betreuer] Vogeser. "Investigation and development of stable isotope dilution mass spectrometry methods for therapeutic drug monitoring of anti-infective drugs used in the critically ill / Carina Schuster ; Betreuer: Michael Vogeser." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2020. http://d-nb.info/1212362896/34.

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Haynes, Christopher Allen. "Development of an assay for fatty acyl-CoAs using liquid chromatography-electrospray ionization-tandem mass spectrometry and its application to the stable isotope labeling and quantitation of sphingolipid metabolism." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37171.

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Fatty acyl-Coenzyme As are metabolites of lipid anabolism and catabolism. A method was developed for their quantitation in extracts of cultured mammalian cells using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Palmitoyl-CoA (C16:0-CoA) is utilized for de novo sphingolipid biosynthesis catalyzed by serine palmitoyltransferase (SPT), which condenses palmitoyl-CoA and serine to form 3-ketosphinganine. After reduction to form sphinganine (Sa), dihydroceramide synthase (CerS) can N-acylate the Sa using a second fatty acyl-CoA molecule, forming dihydroceramide (DHCer). The CerS enzyme family utilizes different acyl chain lengths of fatty acyl-CoAs in an isoform-specific manner, resulting in DHCer with N-acyl chains ranging from C16 to C26 [and even longer] in mammalian tissues. DHCer is trans-4,5-desaturated to yield ceramide, which is further metabolized by the addition of moieties at the 1-O-position, forming sphingomyelin (SM) and ceramide monohexose (CMH). The rates of fatty acyl-CoA and sphingolipid biosynthesis were determined using stable isotope-labeling and LC-ESI-MS/MS analysis of the analyte isotopologues and isotopomers. Isotopic labeling of palmitoyl-CoA with [U-13C]-palmitate in HEK293 and RAW264.7 cells was robust and rapid (~ 60% labeling of the metabolite pool in 3 hr). Isotopic labeling of sphingolipids indicated utilization of [M + 16]-palmitoyl-CoA by SPT and CerS isoforms in both cell types. Metabolic flux modeling was applied to the data for [U-13C]-palmitate activation to [M + 16]-palmitoyl-CoA and its subsequent utilization in de novo sphingolipid biosynthesis, and this analysis indicated rapid turn-over rates for palmitoyl-CoA and ceramide in both cell types. Palmitate treatment of cultured cells alters their metabolic status and gene expression, therefore labeling of palmitoyl-CoA by treatment with [1-13C]-acetate was employed. A distribution of mass-shifted palmitoyl-CoA species (isotopologues) is observed based on the number of incorporations of [1-13C]-acetate during de novo biosynthesis, requiring computational analysis to derive two parameters: the isotopic enrichment of the precursor pool, and the fraction of palmitoyl-CoA that was biosynthesized during the experiment. Previous reports by others describe mass isotopomer distribution analysis (MIDA) and isotopomer spectral analysis (ISA) for this purpose, and both calculation approaches indicated concurrent results. In summary, the quantitation of fatty acyl-CoAs and their isotopic enrichment during stable isotope-labeling studies of lipid metabolism can provide data that significantly change the interpretation of analyte quantitation in these experiments, as demonstrated here for investigations of de novo sphingolipid biosynthesis.
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Books on the topic "Stable isotope dilution assay"

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Trincherini, P. R. Precise determination of magnesium and lutetium in input accountability tank solutions of nuclear reprocessing plants by the mass spectrometric stable isotope dilution technique. Luxembourg: Commission of the European Communities, 1986.

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Porter, L. K. 15N techniques and analytical procedures: Indo/U.S. science and technology initiative. 1992.

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Book chapters on the topic "Stable isotope dilution assay"

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Schmarr, Hans-Georg. "Stable Isotope Dilution Assay." In Food Aroma Evolution, 241–58. 1st edition. | Boca Raton : CRC Press, 2019. | Series: Food analysis & properties, 2475-7551: CRC Press, 2019. http://dx.doi.org/10.1201/9780429441837-12.

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Rychlik, Michael, and Stefan Asam. "Quantitation of Trichothecene Mycotoxins by Stable Isotope Dilution Assays." In ACS Symposium Series, 252–65. Washington, DC: American Chemical Society, 2008. http://dx.doi.org/10.1021/bk-2008-1001.ch013.

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Flaig, Mario, and Michael Granvogl. "Quantitation ofcis- andtrans-3,5-Diethyl-1,2,4-trithiolanes in CookedAlliumVarieties Using a Stable Isotope Dilution Assay." In ACS Symposium Series, 109–22. Washington, DC: American Chemical Society, 2015. http://dx.doi.org/10.1021/bk-2015-1212.ch008.

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Rychlik, Michael. "Stable Isotope Dilution Assays in Vitamin Analysis-A Review of Principles and Applications." In Fortified Foods with Vitamins, 1–19. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2011. http://dx.doi.org/10.1002/9783527634156.ch1.

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Rychlik, Michael. "Quantitation of Pantothenic Acid in Fortified Foods by Stable Isotope Dilution Analysis and Method Comparison with a Microbiological Assay." In Fortified Foods with Vitamins, 123–33. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2011. http://dx.doi.org/10.1002/9783527634156.ch9.

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Blank, Imre, Christian Milo, Jianming Lin, and Laurent B. Fay. "Quantification of Aroma-Impact Components by Isotope Dilution Assay—Recent Developments." In Flavor Chemistry, 63–74. Boston, MA: Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-4693-1_7.

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Van Wouwe, J. P., and R. Rodrigues Pereira. "Stable Isotope Dilution for Zinc Analysis in Low Birth Weight Infants." In Biological Trace Element Research, 344–49. Washington, DC: American Chemical Society, 1991. http://dx.doi.org/10.1021/bk-1991-0445.ch027.

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Traitler, H., U. Richli, A. M. Kappeler, H. Winter, R. Munoz-Box, and N. Fournier. "Quantitative Determination of Prostanoids by Stable Isotope Dilution Gas Chromatography / Mass Spectrometry." In Dietary ω3 and ω6 Fatty Acids, 323–32. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4757-2043-3_29.

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Milo, Christian, and Imre Blank. "Quantification of Impact Odorants in Food by Isotope Dilution Assay: Strength and Limitations." In ACS Symposium Series, 250–59. Washington, DC: American Chemical Society, 1998. http://dx.doi.org/10.1021/bk-1998-0705.ch022.

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Fang, Mingchih, and Keith R. Cadwallader. "Improved Synthesis of Deuterium-Labeled Alkyl Pyrazines for Use in Stable Isotope Dilution Analysis." In ACS Symposium Series, 57–69. Washington, DC: American Chemical Society, 2012. http://dx.doi.org/10.1021/bk-2012-1098.ch005.

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Conference papers on the topic "Stable isotope dilution assay"

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GRIFFIN, HENRY C., JAMES E. MARTIN, and CHUL LEE. "ASSAY OF Hg IN LARGE ELECTROCHEMICAL CELLS BY ISOTOPE DILUTION." In Proceedings of the 3rd International Conference on Isotopes. WORLD SCIENTIFIC, 2000. http://dx.doi.org/10.1142/9789812793867_0026.

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Ersdel, E., M. Andersson, and S. Rosen. "DETERMINATION OF SOLUBLE FIBRIN IN PLASMA WITH A CHR0M0GENIC KIT METHOD UTILIZING THE HIGH AFFINITY PLASMIN SUBSTRATE S-2390." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643058.

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Abstract:
A sensitive and quantitative assay of soluble fibrin is of clinically diagnostic relevance in an early thrombotic state where there is a risk for development of DIC. Recently Wiman and Ranby (Thromb. Haemostas 55, 189-193 (1986)) published a spectro-photometric assay which met these criterions. The single-stage assay procedure is based upon activation of Glu-Plasminogen to Plasmin by t-PA in the presence of soluble fibrin and hydrolysis of the chromogenic plasmin substrate S-2390, H-D-Val-Phe-Lys-pNA, which has a high affinity for plasmin. The rate of plasmin generation is correlated to the amount of soluble fibrin monomers present in the sample.A complete kit containing optimized, stable reagents has now been developed which allows a quantitative determination of soluble fibrin in the range 30-200 nmol/1 within 30 min. at room temperature (20-25°C). The assay procedure is straightforward involving addition of 200 pi diluted plasma sample to 200 pi Glu-Plasminogen and 100 ul of a t-PA/S-2390-reagent.The results show a high resolution of the standard curve as illustrated by a AA405 amounting to about one absorbance unit between a 200 nmol/1 sample of soluble fibrin and the reagent blank, some variation, ±0.1 absorbance unit, being caused mainly by differences in temperature. In combination with an intra-assay variation coefficient = 6.3% and 5.0% at 150 and 50 nmol/1, respectively, this will allow safe and reliable differentiation of pathological levels of soluble fibrin from levels found in healthy subjects (below 10 nmol/1). A similar precision is also obtained when the assay is performed in microplates.In the original procedure fresh frozen human plasma was utilized as a dilution medium for soluble fibrin. Comparisons with carefully collected bovine plasma proved this source to be a convenient substitute. Furthermore, lyophilization of the bovine plasma did not produce any significant degradation of fibrinogen which otherwise might interfere in the assay. This simple kit procedure should make it a suitable tool in early determinations of soluble fibrin in a number of pathological states which may result in severe haemostatic disturbances.
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