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Athan, Donna Michelle, and n/a. "Topographic distribution of human brain activity associated with cognitive processing in anxiety disorders." Swinburne University of Technology, 2006. http://adt.lib.swin.edu.au./public/adt-VSWT20060904.115700.
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Increased attention towards threatening stimuli in both the external and internal
environments is thought to be a factor in the causation and maintenance of pathological
anxiety. Attentional biases for threatening information have been demonstrated in anxiety
disorders, however the cortical mechanisms involved remain unclear. In this investigation,
an Emotional Stroop task consisting of neutral, positive, depression-related and anxietyrelated
words, was used to investigate attentional biases in 14 Panic Disorder patients and
32 psychiatrically healthy controls. The standard colour-word Stroop was also performed
to determine whether any general cognitive deficits exist in Panic Disorder. Steady-state
probe topography (SSPT), a brain electrical activity imaging methodology, was used to
investigate participants' brain activity during performance of the tasks. It was hypothesised
that Panic Disorder is associated with specific biases for disorder-specific information and
thus patients would exhibit increased interference for anxiety-related words only, compared
to neutral words. Mean reaction times for the Standard Stroop was similar for the two
groups. For the Emotional Stroop task, neither group showed an interference effect for any
emotional category. However, Panic Disorder patients performed the Emotional Stroop
significantly more slowly than the Controls. The SSPT data suggest that the Standard and
Emotional Stroop tasks are associated with different patterns of brain activity in the Control
and Panic Disorder groups despite the similarities in the reaction time data. Specifically,
the Standard Stroop was marked by strong temporo-parietal excitation in the Panic Disorder
group only. In addition, anterior SSVEP patterns further differentiated between the Control
and Panic Disorder groups. The most striking finding for the Emotional Stroop was strong
sustained bilateral temporo-parieto-occipital excitation in the Panic Disorder group. In
addition, a subgroup of the Controls exhibited increased interference for anxiety-related
words and therefore the brain activity for this group and the remainder of Controls who did
not show interference was analysed separately. It was found that the presence of
interference for anxiety-related words was associated with right prefrontal inhibition prior
to response. Other time-varying changes in the SSVEP further distinguished between the
subgroup of Controls who showed an interference effect and those who did not.
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Coates, Elizabeth Christine. "Analysis of Drosophila ssp4." Thesis, University of Kent, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.520906.
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CAMSAP 1 is a spectrin associated, Calmodulin regulated protein that is a member of a large ubiquitous family of cytoskeletal proteins, defined by a novel C-terminal domain, the CKK domain. The CAMSAP family member in the fruit fly Drosophila melanogastor is encoded by the gene short spindles4 (ssp4). Little is known about this gene but a role in microtubule dynamics has been shown in cultured cells. I have interrogated bioinformatics databases and compared Ssp4 with Human CAMSAP proteins and found many similarities, and some differences, between them. Using in situ hybridisation I show that ssp4 transcripts are expressed in the gut, head and central nervous system during embryogenesis. An antibody that recognises the Ssp4 C-terminus reveals expression throughout the embryonic development of the gut and nervous system, and in a discrete population of cells in the head. I have investigated the effects of two independent P-element induced mutant alleles of ssp4 and show that mutant flies die in late embryonic or early larval stages. Disruptions to the locus do not seem to affect the nervous system but mutants were found to have aberrant head involution. I present preliminary evidence that suggests this defect may be the result of reduced apoptosis in the embryo. Head involution is a complex process, dependent upon co-ordinated changes to cell shape and the movement of groups of cells from different origins. As Ssp4 is a multi-domain cytoskeletal protein that is required for embryonic development, it may play a role in processes that are common to these morphogenetic events.
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Mameesh, Rania Hussein. "Symbiotic Subordinate Threading (SST)." College Park, Md. : University of Maryland, 2007. http://hdl.handle.net/1903/6732.
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Thesis (Ph. D.) -- University of Maryland, College Park, 2007.Thesis research directed by: Electrical Engineering. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
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Lundquist, Tomas. "FEM-modelling of SSRT for Corrosion Tests." Thesis, Linköping University, Applied Mathematics, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-58920.
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This thesis discusses the mathematical formulation and computational treatment of slow strain rate corrosion tests based on nonlinear finite elements methods. The theory is illustrated by a description of classical small strain elastoplasticity theory as implemented in the Comsol Multiphysics 3.2 software package. The possible extension of the theory to finite strain is briefly addressed. Practical simulations and results regarding the evolution of stresses, strains and geometric deformation are also presented and discussed. Experimental data used in simulation where reported by Onchi, Takeo et al. and published in Journal of Nuclear Science and Technology in May 2006.
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Söderberg, Christina. "Individriktad brottsprevention : om SSPF och viljan att samverka." Licentiate thesis, Linköpings universitet, Institutionen för samhälls- och välfärdsstudier, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-125927.
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Denna licentiatuppsats behandlar brottspreventiv samverkan på individnivå, vilket är ett relativt nytt fenomen i Sverige och Skandinavien. Ett specifikt arbetssätt, samverkan mellan skola, socialtjänst, polis och fritidssektor – SSPF – undersöks i studien och övergripande studeras hur idén om brottspreventiv samverkan i form av SSPF konstrueras och motiveras, hur den tolkas och omtolkas av samverkansparterna samt hur den omsätts i praktik. Det mer specifika syftet med studien är att undersöka brottspreventiv samverkan på individnivå som idé och praktik. Tre olika fokus eller aspekter på detta belyses: policy/struktur; organisation/profession och praktik/familj. De frågeställningar som besvaras i studien och som är kopplade till ovanstående fokusområden är följande: Hur har denna form av samverkan runt unga växt fram och hur motiveras den? Hur ser de organisatoriska förutsättningarna ut och hur upplevs SSPF av samverkansparterna? Slutligen hur interagerar företrädare för samhällets institutioner/ de professionella med familjen? Tre kommuner ingår i undersökningen. Empiriskt material har samlats in genom dokumentstudie, intervjuer i grupp och enskilt med samverkansparterna i de tre kommunerna, genom observationer vid SSPF-möten samt genom en enkät som gick ut till 30 kommuner och stadsdelar i Sverige. Resultaten visar att en koppling finns mellan den allmänna samhällsutvecklingen i stort och brottspreventionens utveckling. Allmänt kan sägas att talet om brottsprevention idag handlar allt mindre om strukturella förklaringar och åtgärder. I stället talas alltmer om kontroll och effektivitet, vilket bland annat innebär tidiga upptäckter och snabba insatser. För att lyckas med detta behöver samverkan komma till stånd. Resultaten visar också att polisens roll i individriktad prevention har blivit viktigare och framträder som den tydligaste aktören i SSPFsamverkan. Gällande förutsättningarna för SSPF-arbetet visar studien att såväl organisatoriska förutsättningar som exempelvis stabilitet i organisationer och implementeringsmöjligheter, som möjligheter till informationsutbyte på individnivå mellan parterna spelar stor roll för arbetet. De professionella bildar allians där den gemensamma bilden av den unge skapar mening. En spänning mellan stöd och kontroll blir synlig i SSPF-arbetet, likväl som en sorts likriktning, där poliser agerar socialarbetare och socialtjänsten talar om mer kontroll. Allians bildas också mellan professionella och föräldrar, vilket främst syftar till ökad kontroll runt den unge, men också till att stärka stödet till den unges föräldrar. De professionellas strävan efter ett nytt sätt att arbeta för att göra det bättre för målgruppen blir trots allt tydligt i studien.This dissertation deals with crime prevention co-operation targeted at the individual level, a relatively new phenomenon in present Sweden and Scandinavia. A certain work model – SSPF, collaboration between school, social services, the police and the leisure sector – is chosen for the study. The study's purpose is to investigate crime prevention co-operation on individual level as idea and practice. To this end, different focuses are used: policy/structure; organization/profession and practice/family. The questions the study answers attached to the focus areas above are the following: How has this form of interaction around the young emerged and how is it justified? What are the organizational conditions and how is SSPF experienced by collaborative partners? What does the interaction look like between those representing society´s institutions as well as between the professionals and the families? Three municipalities are examined in the study. The empirical material was collected through studies of documents; interviews, in groups and individually, with collaborative partners in the three municipalities; observations at SSPF-meetings and a survey that were sent to a number of municipalities and districts in Sweden. On an overall level, the results show that a clear link exists between the general development of society at large and the development of crime prevention work. Generally it can be said that talk of crime prevention today is less focused on structural explanations and action, but rather increasingly concerns control and efficiency, including early discoveries and rapid reaction, which should be strengthened through collaboration at work. The role of the police in individually targeted prevention has become more important and they can be seen clearly as the most dominant party in the SSPF-interaction. Regarding the current conditions for SSPF-work, the study shows that both the organizational prerequisites (such as stability in organizations), and implementation (such as opportunities for exchange of information at the individual level between the parties) play a major role. The professionals form an alliance in which the common image of the young creates meaning. A tension between support and control becomes visible in the work, as well as a kind of uniformity – iso-morphism- whereby the police acting social workers and the social service talk about more control. An alliance is also formed between professionals and parents, aiming mainly at gaining increased control concerning the young person, but also at strengthening support to the parents. The professional´s quest for a new way of working to make it better for the target group becomes clear in the study. In conclusion: this kind of collaborative work within the area of crime prevention is complex, and this study is a contribution to a better understanding of the subject.
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Engert, Nadine [Verfasser]. "Phenolic acids and antioxidative capacity on ancient wheat namely einkorn (T. monococcum ssp.), emmer (T. turgidum ssp.) and spelt wheat (T. aestivum ssp. spelta) and on germinated bread wheat (T. aestivum ssp. aestivum) / Nadine Engert." Gießen : Universitätsbibliothek, 2011. http://d-nb.info/1063177758/34.
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Fredriksson, Sara, and Anna Schmidt. "Where are you? : A qualitative investigation of self-service technology in the hotel industry." Thesis, Umeå universitet, Företagsekonomi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-161229.
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Throughout the last years, the service encounter has gone through drastic changes due to rapid technological developments. The research area of service marketing is therefore putting a stronger focus on the academic field of technology-infused service encounters. Marketers have moreover also started to implement self-service technology (SST) within their service encounters, in order enhance their service delivery. This phenomenon has also been visible within the hospitality industry, whereby the hotel industry has experienced an increasing adoption of SST encounters. Consequently, hotels’ implementation of SST kiosks has enhanced the hotel guests’ participation level within the service encounter. Therefore, this thesis aims to gather insights on the emerging SST customer roles, by investigating the guests’ attitude towards the SST encounter. This leads to the investigation of distinctive SST customer roles within hotels’ self-check-ins. The choice of the research topic was driven by the fact that existing research about SST implementation in the service encounter, lacks an investigation of the customers’ perspective. Thereby, a research gap was identified that outlays the customers’ enhanced participation as service co-producers. Previous research has focused on investigating customers’ technology acceptance, rather than their own identification as co-producers. Thereof, this thesis will put an emphasis on hotel guests’ attitude towards SST, as a specific aspect of the technology acceptance process. Moreover, this thesis will focus its investigation on self-check-ins within hotels that do not incorporate a human interaction point for their guests. Consequently, in order to investigate the SST customer roles more thoroughly, this thesis will also consider the viewpoint of the hotel. Therefore, qualitative interviews among nineteen hotel guests and one hotel representative were conducted. The findings of this thesis revealed that SST customer roles cannot be predetermined within a hotel self-check-in. Instead, the hotel guests define their own SST customer roles through a reflection upon their purpose of the hotel stay, expectations of the check-in encounter, arising special needs and perceived benefits of the self-check-in. This determines their individual ‘role file card’, which can be utilized into the proposed SST customer roles archetypes of: technology enthusiast, beneficiary, traditionalist and contradictor. In regard to that, it has been identified that the information provided prior to the hotel guests’ stay, influences the reflection upon their SST customer roles. Moreover, it was discovered that with their increased responsibility over the check-in process, the hotel guests identified themselves as co-producers. Therefore, the findings outlined that the guests would want the price of the hotel stay to reflect their increased involvement in the service delivery. From a theoretical perspective this thesis bridged the identified research gap of investigating hotel guests’ attitude towards SST, in order to classify the different customer roles that arise within a hotel’s self-check-in. Moreover, the findings revealed practical implications for hotel managers in regards of the need to provide their guests with more information about the SST encounter. Thereby, hotel managers will be enabled to increase their guests’ satisfaction with the SST encounter, as the guests’ expectations would be coherent with the hotel’s offerings.
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Ding, Birte [Verfasser]. "Vergleichende Analyse der Blütentranskriptome von Vitis vinifera ssp. sylvestris und Vitis vinifera ssp. vinifera / Birte Ding." Mainz : Universitätsbibliothek Mainz, 2016. http://d-nb.info/1120445663/34.
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Dipesh, Dugar Deepika. "Public Self Service Technology (SST): Designing for Trust : Factors enhancing user’s trust towards a public SST." Thesis, KTH, Skolan för elektroteknik och datavetenskap (EECS), 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-238690.
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Public Self Service Technology (SST) has become an important part of our daily life. Advancement in technology and reduced hardware costs have motivated service providers to deploy public a SST for various important and complex tasks. Examples of such tasks include editing and printing confidential documents, performing monetary transactions, etc. These tasks requires a user to reveal his/her personal information to a public SST. The major problem while performing these tasks using a public SST is that the user has to deal with many surrounding factors like social density, privacy and security, which might affect his/her trust towards the SST and in turn the user might refrain from using it. This study aims to find different factors that can enhance user’s trust towards a public SST, encourage to use it and complete the task even if it requires user’s personal information.The in-depth interview method was adopted for the study to learn twelve interviewees’ experience with varied public SSTs that specifically handles personal information, in an urge to understand interviewees’ behaviour, underlying motivations and desires to use those public SSTs. Analysing the data collected from interviews, ten trust factors were found that emerge at various stages of interaction with a public SST. They were categorized into pre-interaction, on-interaction and post-interaction factors based on their time of interaction. Beyond the trust factors, three additional important factors have emerged from interview data, which motivates users to adopt public SSTs. They are usefulness, convenience and personality-based trust. The results may be valuable for researchers who are focusing on different aspects of trust and any public artefact as well as for service providers and designers to design a trustworthy public SST. Based on the results, some practical implications for designing public SSTs are also presented.Offentlig självbetjäningsteknik (eng. Public Self Service Technology) har blivit en viktig del av vårt dagliga liv. Förbättring i teknik och minskade hårdvarukostnader har motiverat tjänsteleverantörer att distribuera offentliga SST för olika viktiga och komplexa uppgifter. Exempel på sådana uppgifter är att redigera och skriva ut konfidentiella dokument, utföra monetära transaktioner etc. Dessa uppgifter kräver att en användare delar med sig av personlig information till en offentlig SST. Det stora problemet med att utföra dessa uppgifter med hjälp av SST är att användaren måste hantera många omgivande faktorer som social täthet, integritet och säkerhet, vilket kan påverka personens förtroende mot SST och i sin tur kananvändaren avstå från att använda den. Följaktligen syftar denna studie till atthitta olika faktorer som kan öka användarnas förtroende mot offentliga SST, uppmuntra att använda dem och slutföra uppgifter även om det kräver användarens personuppgifter.En djupintervjumetod användes i studien för att lära sig om tolv testdeltagares erfarenhet av olika offentliga SST, specifikt de som hanterar privat information, i en strävan att förstå deltagarnas beteende, underliggande motivation och önskemål att använda dessa offentliga SST. Analys av de uppgifter som samlats in från intervjuer, upptäckte tolv förtroendefaktorer som uppstår vid olika stadier av interaktion med en offentlig SST. De var därför uppdelade i pre-interaktion, interaktionsoch post-interaktionsfaktorer. Från intervjuerna har ytterligare tre viktiga faktorer upptäckts som motiverar användarna att använda offentliga SST. De är användbarhet, bekvämlighet och personlighetsbaserat förtroende. Resultaten är värde-fulla för forskare som fokuserar på olika aspekter av förtroende och offentligaartefakter samt för tjänsteleverantörer och designers för att utforma en pålitlig offentlig SST. Baserat på resultaten presenteras också några praktiska konsekvenser för utformningen av offentliga SST.
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Hersch, Greg Louis. "ClpX interactions with ClpP, SspB, protein substrate and nucleotide." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/34199.
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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, February 2006.Includes bibliographical references.
ClpXP and related ATP-dependent proteases are implements of cytosolic protein destruction. They couple chemical energy, derived from ATP hydrolysis, to the selection, unfolding, and degradation of protein substrates with the appropriate degradation signals. The ClpX component of ClpXP is a hexameric enzyme that recognizes protein substrates and unfolds them in an ATP-dependent reaction. Following unfolding, ClpX translocates the unfolded substrate into the ClpP peptidase for degradation. The best characterized degradation signal is the ssrA-degradation tag, which contains a binding site for ClpX and an adjacent binding site for the SspB adaptor protein. I show that the close proximity of these binding elements causes SspB binding to mask signals needed for ssrA-tag recognition by ClpX. The SspB dimer overcomes this signal masking by tethering itself and bound substrate to ClpX, via docking sites located in the dimeric N-terminal domain of ClpX. Because this N-domain dimer binds only a single SspB subunit, the ClpX hexamer can accommodate just one SspB dimer per hexamer. Other adaptor proteins that use these same tethering sites must compete with SspB for access to ClpXP. Substrates bearing ssrA tags with increased spacing between the SspB and ClpX binding elements are degraded more efficiently at low concentrations by ClpXP.
(cont.) This mechanism in which the adaptor first obstructs and then stimulates substrate recognition may have evolved to permit an additional level of regulation of substrate choice. SspB binding to ssrA-tagged substrate is a highly dynamic process, allowing rapid transfer of substrates from SspB to ClpX. Although the ClpX hexamer is composed of six identical polypeptides, individual subunits assume at least three distinct conformations. Using a hexamer that was engineered to prevent nucleotide hydrolysis, I show that some nucleotide-binding sites in ClpX release ATP rapidly, others release ATP slowly, and at least two sites remain nucleotide free. Occupancy of both the slow sites by ATP and the fast sites by either ATP or ADP is required to bind the degradation tags of protein substrates. The ability of ClpX to retain binding of substrate with ATP or ADP in the fast sites suggests that nucleotide hydrolysis in the fast sites, but not in the slow sites, will allow repeated unfolding attempts without substrate release over multiple ATPase cycles. My results rule out ATPase models including ClpX6eATP6 or ADP6 and also suggest that the enzyme hydrolyzes only a fraction of bound ATP in a single turnover event. Short peptide motifs of ClpX, known as IGF loops, interact with ClpP and change conformation as a response to nucleotide binding by ClpX.
(cont.) As ClpX varies its nucleotide content during the ATP hydrolysis cycle, it also varies its affinity for ClpP. Processing of substrates is coupled to the ATP-hydrolysis cycle of ClpX and appears to modulate ClpX's affinity for ClpP by changing how long each ClpX subunit spends in each nucleotide state.
by Greg Louis Hersch.
Ph.D.
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Forsgren, Nina. "Structural studies of the surface adhesin SspB from Streptococcus gordonii." Doctoral thesis, Umeå : Umeå universitet, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-32910.
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Stein, Katrin. "Entwicklung einer Realtime-PCR zum Nachweis von Mycobacterium avium ssp. avium und Mycobacterium avium ssp. silvaticum beim Vogel." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-136020.
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Sharif, Nadder. "Somatostatin receptor 5 (sst₅)-regulated trafficking of somatostatin receptor 2A (sst₂A) in epithelial and pituitary cell lines." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98797.
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The purpose of the present study was to characterize the mechanisms by which cells that express both sst2A and sst5 receptors remain responsive to SRIF analogs under continuous stimulation. Hypothesizing that the expression of sst5 affects the regulation of sst 2A, we compared the agonist-induced internalization and intracellular trafficking of sst2A receptors in cells expressing sst2A alone and together with sst5. In cells stably expressing sst 2A, stimulation with a non-selective SRIF agonist ([D-Trp8]-SRIF-14) and a sst2-selective agonist (L-779,976) induced sst2A internalization and decreased responsiveness to further agonist stimulation. By contrast, in cells co-expressing sst2A and sst5, while stimulation with [D-Trp8]-SRIF-14 similarly induced sst 2A internalization and loss of cellular responsiveness, L-779,976 stimulation resulted in a maintenance of surface receptor density, and consequently, continued responsiveness to [D-Trp8]-SRIF-14. Taken together, our results suggest that sst5 exerts an influence on the regulation of sst 2A, such that cells expressing these receptor subtypes are able to remain responsive to SRIF agonists under continuous exposure.
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Fustier, Margaux-Alison. "Adaptation locale des téosintes Zea mays ssp. parviglumis et Zea mays ssp. mexicana le long de gradients altitudinaux." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS147/document.
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Les téosintes Zea mays ssp. parviglumis et ssp. mexicana sont les apparentés sauvages les plus proches du maïs cultivé. Elles occupent au Mexique des niches écologiques distinctes bien délimitées par l’altitude. Zea mays ssp. parviglumis pousse dans des environnements chauds et humides situés à moins de 1800m d’altitude, tandis que Zea mays ssp mexicana pousse dans des environnements plus secs et froids (au-dessus de 1800m). Nous nous sommes intéressés aux bases génétiques de l’adaptation locale des téosintes à l’altitude. Dans ce but, 37 populations ont été échantillonnées le long de deux gradients altitudinaux, utilisés comme réplicats biologiques. Deux populations extrêmes de chaque gradient ainsi que deux populations de moyenne altitude de chaque sous-espèce du gradient 1 ont été séquencées à faible profondeur afin de découvrir des polymorphismes dont les fréquences alléliques sont contrastées entre les extrêmes tout en contrôlant pour la différenciation entre les sous-espèces. A partir de méthodes de détection de la sélection intra- et inter-populationnelle, des locus candidats ont été détectés. Une revue bibliographique a permis d’établir des correspondances entre nos candidats et des régions précédemment décrites pour leurs effets phénotypiques sur des caractères adaptatifs. Nous montrons ainsi un rôle important des interactions sol-plante et de la pilosité des feuilles dans l’adaptation à l’altitude. Pour valider certains de nos candidats, 270 polymorphismes ont été génotypés sur les 37 populations afin de réaliser des études de clines de fréquence. Parallèlement, nous avons mis en œuvre un dispositif de caractérisation phénotypique (2 lieux x 2 années) afin de tester l’association entre ces polymorphismes et 18 caractères mesurés en champ. Nous discutons des apports méthodologiques de notre étude aussi bien du point de vue des technologies haut débit que de la détection de la sélection. Notre dispositif devra être pleinement exploité pour valider nos candidats. Les perspectives incluent la poursuite de l’étude sur d’autres types de polymorphismes ainsi qu’un suivi temporel des populationsTeosintes Zea mays ssp parviglumis (parviglumis) and Zea mays ssp mexicana (mexicana) are the closest wild relatives of cultivated maize. They occupy distinct ecological niches in Mexico, well separated by altitude. Zea mays ssp. parviglumis encountered below 1800m grows in wet and warm conditions, while mexicana grows in drier and colder climates (above 1800m). In order to investigate the genetic bases of local adaptation to altitude, we sampled 37 populations along two altitudinal gradients. We sequenced the two most extreme populations of each gradient as well as two intermediate populations - one from each subspecies along gradient 1. We searched for polymorphisms with contrasted allele frequencies between the extremes while accounting for subspecies differentiation. Using both inter- and intra- population methods we identified several candidate loci. Based on a literature review, we confronted them with regions previously described as involved in phenotypic variation of adaptive traits. Our results highlight the role of plant-soil interactions and leaves hairiness in the adaptation to altitude. To validate further a subset of 270 polymorphisms chosen among our best candidates, we genotyped them on the 37 populations with the aim of performing clinal analyzes of allele frequencies. In parallel, we undertook phenotypic evaluation trials (2 locations x 2 years) to test the association of these polymorphisms with 18 traits measured in the field. We discuss the methodological contributions of our study both from the standpoint of high throughput technologies and detection of selective footprints. Our setting will be fully exploited to validate our candidates. Perspectives include the discovery and assessment of the contribution of other types of polymorphisms and the temporal follow-up of populations
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af, Ugglas Carl, and Raqiq Hyder. "To SST, or not to SST, that is the question : En studie om self-service technology inom hotellbranschen." Thesis, Uppsala universitet, Företagsekonomiska institutionen, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-412991.
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Purpose: This study examines how hotels manage digitization within the industry. Specifically it seeks to examine how managers view decisions and strategies for implementing SST in relation to hotel guest’s acceptance. Design/methodology/approach: This study has assumed a mixed methods methodology. The method has been carried out through an abductive approach between theoretical and empirical material. The study is based on a combined research design of qualitative and quantitative nature and is grounded on the empirical evidence that consists of three semi-structured interviews and surveys. The qualitative part leads to analysis according to the theoretical structure and is then combined with statistical analysis of the survey responses. Findings: This study confirms a few elements of previous literature: implementations require considerable investments and costs; SST on offer in the hotel industry are found lacking; guests prefer technologies they have experience with; and managers have a wait-and-see attitude to SST. The study also shows that people used to technology are proportionately big and have a positive attitude towards SST. Further, the study presents new theoretical insights: high-end hotels are expected to offer technology; SST is perceived to reduce hotels security; attitude towards SST does not differ between genders; and SST is perceived to affect aesthetics.Syfte: Denna studie undersöker hur hotell hanterar digitalisering av branschen. Mer specifikt undersöker denna studie hur managers betraktar beslut och strategier för implementering av SST i relation till hotellgästers acceptans. Design/metod/tillvägagångssätt: Denna studie har utgått från metodkombination. Metoden behandlas genom ett abduktivt tillvägagångssätt mellan det teoretiska och empiriska materialet. Studien baseras på en kombinerad forskningsdesign av kvalitativ och kvantitativ natur som består av tre semistrukturerade intervjuer samt efterföljande enkät. Den kvalitativa delen utmynnar i en analys enligt den teoretiska strukturen och kombineras sedan med statistiska analyser av enkätsvaren. Bidrag: Ett antal faktorer som resultatet påvisar bekräftar tidigare forskning: implementationer innebär höga kostnader och investeringar; utbudet av SST inom hotellbranschen är dåligt; gäster föredrar de tekniker de är vana vid; samt att managers har en vänta-och-se attityd till SST. Samtidigt visar studien på att andelen teknologivana människor är stor och att dessa är positivt inställda till SST. Studien bidrar även med nya teoretiska insikter såsom: att premiumhotell förväntas kombinera high-touch med high-tech; att SST upplevs minska hotellens upplevda säkerhet; att inställning till SST inte skiljer sig mellan kön; samt att viss SST upplevs påverka estetiken.
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Neto, José Pedro Prezotto. "Estudo comparativo de venenos de serpentes do gênero Crotalus ssp." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-30012019-075833/.
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As cascavéis são classificadas como grupo monofilético contendo dois gêneros descritos ao grupo: Crotalus ssp. e Sistrurus ssp., os quais surgiram no México a aproximadamente 20 milhões de anos, colonizando então, praticamente todo o continente americano. Fatores como dieta, dimorfismo sexual, ontogenia, mutações e distribuição geográfica podem influenciar na composição dos venenos e consequentemente no envenenamento. O presente trabalho tem como objetivo caracterizar o perfil proteico, bem como as propriedades enzimáticas e imunológicas dos venenos de algumas espécies e subespécies de Crotalus ssp. (C. atrox, C. scutulatus scutulatus, C. viridis viridis, C. vegrandis, C. durissus cascavella, C. d. collilineatus e C. d. terrificus). Os resultados indicaram pouca variabilidade entre os perfis eletroforéticos dos venenos, contudo as diferenças foram na concentração relativa das proteínas. A análises proteômica identificou alguns componentes dos venenos e serinopeptidases, metalopeptidases e fosfolipases A2 foram as mais abundantes. Além disso, por zimografia, observou-se que todos os venenos analisados apresentaram atividade proteolítica e que os venenos norte-americanos em todos os zimogramas foram mais hidrolíticos. Em caseína, a atividade enzimática dos venenos foi menos intensa comparado aos outros substratos. Em relação às gelatinases das amostras estudadas, pôde ser observado inibição da atividade enzimática induzida por alguns componentes utilizando EDTA, principalmente nos venenos de C. atrox e C. vegrandis. Em relação à inibição das serinopeptidases, foi observado que todas as gelatinases dos venenos crotálicos apresentaram inibição total ou parcial da atividade hidrolítica. Houve variabilidade entre as hialuronidases encontradas dos venenos crotálicos, tanto em relação à massa das enzimas e intensidade da degradação, quanto em diferentes pHs. Nos ensaios enzimáticos quantitativos (azocaseinolítico fosfolipásico e peptidásico) os venenos Norte Americanos demonstraram conter mais proteases em relação aos venenos Sul Americanos. Por Western Blotting, as amostras reagiram com os anticorpos presentes nos soros anti-crotálico e anti-botrópico, apresentando reatividade antigênica cruzada entre as amostras homólogas e heterólogas. Além disso, houve imunoreatividade entre o soro anti-jararagina e alguns componentes de todos os venenos crotálicos norte-americanos.The rattlesnakes are classified as a monophyletic group containing two genera referring to the group: Crotalus ssp. and Sistrurus ssp., which arose in Mexico 20 millions of years ago, colonizing then, practically all the American continent. Some scientific works indicate that factors such as diet, sexual dimorphism, ontogeny, mutations and distribution may influence the composition of the venoms and consequently the poisoning. The present work aims to characterize the enzymatic and immunological properties of the venoms of some species and subspecies of Crotalus ssp. (C. atrox, C. scutulatus scutulatus, C. viridis viridis, C. vegrandis, C. durissus cascavella, C. collilineatus and C. d. terrificus). The results indicated few variability among the electrophoretic profiles of the venoms, however the differences were in the relative concentration of the proteins. The proteomic analysis identified serinopeptidases, metallopeptidases and phospholipases A2, which were the most abundant components of the venoms. In addition, zymography assays indicate that the all the venoms showed proteolytic activity, furthermore, the North American venoms, presented more hydrolysis in all zimograms. The caseinolytic activity was less intense compared with other substrates. Regarding the gelatinolytic activity of the samples, inhibition of the enzymatic activity of some components could be observed using EDTA, mainly in the C. atrox and C. vegrandis venoms. Partial or total inhibition was observed of the serinopeptidases activity of the crotalic gelatinases. Among the hyaluronidases, variations between crotalic venoms, in relation to the enzymes mass and degradation intensity were identified. In addition, when incubated at different pHs, the hyaluronidase profile presented different patterns in the activity. In the quantitative enzymatic assays (azocaseinolytic phospholipasic, peptidasic) the North American venoms displayed higher activity in relation to the South American venoms. In the Western Blotting assays, the samples reacted with antibodies present in the Brazilian anti-crotalic and bothropic sera, indicating cross-reactive antigenicity between the homologous and heterologous samples. Besides that, there was immunoreactivity between the anti-jararrhagin serum and some components of all North American crotalic venoms.
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Pattanavibool, Rungnapar. "Somatic cell genetics in larches, Larix ssp." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0003/NQ32762.pdf.
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Bayindir, Ürün. "Hinokinin biosynthesis in Linum strictum ssp. corymbulosum." Aachen Shaker, 2007. http://d-nb.info/987297767/04.
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Selmer, Inger-Sofie. "The neurobiology of brain somatostatin sst₄ receptors." Thesis, University of Cambridge, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621134.
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Janoštík, Milan. "Návrh olejové nádrže pro turbínu SST 300." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2010. http://www.nusl.cz/ntk/nusl-228911.
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Thesis deals with oil-turbine frame of the model SST 300 SST 400. This work is divided into several parts. Beginning of this thesis deals with a description of the problem and the oil economy. Next part is concerned with 3D design model of the oil tank in the program ProEngineer Wildfire 2.0. Final part describes a creation of manufacturing drawings with BOMs. In conclusion, the thesis presents the results of additional topics, such as a computational model in Ansys 11 and making the calculation of tension or bonds in an oil suspension in the frame. The result of this work is the full-fledged design of the weldment of oil frame, which was submitted for approval to the Siemens Company, which will continue in the subproject tasks of new turbo model SST 300 pattern SST 400.
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Stenudd, Christoffer, and Reza Sepahvand. "Digitala banktjänster : En kvantitativ fallstudie om anammande av självserviceteknik bland svenska bankers privatkunder." Thesis, Umeå universitet, Företagsekonomi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-105378.
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De senaste åren har bankerna genomgått en hastig digital förändring och utvecklingen har inneburit stora omställningar, inte minst för bankernas privatkunder. Framväxten av flertalet nya elektroniska tjänster har inneburit stora förändringar inom finansbranschen. Privatkunder anammar dock denna nya teknik i olika takt, vilket har föranlett intresset att utföra denna studie där vi vill undersöka hur användande av självserviceteknik påverkas av olika aspekter av kundernas tekniknivå.Med detta som bakgrund genomfördes vår studie utifrån följande problemformulering: ”Hur kan svenska banker öka användandet av sina digitala banktjänster bland privatkunder?” Vi har använt oss av tidigare forskning och teorier om teknikberedskap, innovationsanammning, teknikacceptans samt tjänstemarknadsföring, och med hjälp av en kvantitativ studie undersökt hur dessa påverkar användandet av självserviceteknik, (SST) som är kärnan i denna studie.Då vi har undersökt privata bankkunder så har dessa varit grunden i vår kvantitativa undersökning där datainsamlingen skedde via en Webbenkät som besvarats av 185 respondenter. Utifrån enkätsvaren har vi gjort omfattande statistiska och deskriptiva analyser, samt grupperat olika respondenter efter olika faktorer såsom ålder, olika användarkombinationer, inställning till risk, förändring och inflytande, och liknande. För dessa olika grupperingar har vi sedan analyserat skillnader i användande av digitala banktjänster. Genom flertalet statistiska tester och analyser där bland annat chi-två-tester och regressionsanalyser använts har vi funnit förklaringsgrader, och signifikanta variabler utifrån enkätsvaren, som vi med hjälp av korstabeller använt till fler och djupare analyser.Det resultat och slutsatser som studien visar på är att informationsnivån hos bankkunder är för låg för vissa banktjänster, och att denna bör höjas då den har direkt betydelse för anammande av digitala tjänster. Bankerna bör fysiskt demonstrera sina banktjänster på kontoren, samt känna av varje enskild kund, och sälja in tjänsterna på rätt sätt till olika slags kunder, då dessa anammar tekniken i olika takt. Vi har kategoriserat respondenter utifrån studiens teori, och inom dessa grupperingar visat på skillnader i användande av digitala banktjänster.
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Marklund, Simon, Erik Lundin, and Kenny Jakobsson. "Teknikens begränsningar : En studie om självbetjäning inom dagligvaruhandeln." Thesis, Umeå universitet, Institutionen för informatik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-109780.
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The underlying aim of this paper is to pinpoint the perceived problems that customers may encounter while using the Self-service technology at a large grocery store in Sweden, with the purpose to later present these problems to the management of the grocery store to give them the chance to correct these problems. A survey at the grocery store was done to see what the customers really thought about the technology. After the survey was completed there was an interview with two representatives from the grocery store’s headquarter. The purpose of this interview was to give the representatives the information gained from the survey, and interview them about the problems which the customers gave us knowledge about.
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Martínez, Ainsworth Natalia Elena. "Characterizing the genomic determinants and phenotypic responses to altitudinal adaptation in teosintes (Zea mays ssp. parviglumis and ssp. mexicana)." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS376.
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Les deux sous-espèces annuelles de téosinte qui sont les plus proches parents sauvages du maïs sont d’excellents systèmes pour étudier l’adaptation locale car leur distribution couvre un large éventail de conditions environnementales. Zea mays ssp. parviglumis est distribuée dans un habitat chaud et mésique en dessous de 1800 m d’altitude, tandis que Zea mays ssp. mexicana prospère dans des conditions sèches et fraîches à des altitudes plus élevées. Nous avons combiné des approches d’écologie inverse et de génétique association afin d’identifier les déterminants de l'adaptation locale chez ces téosintes. A partir de données de séquençage haut débit (HTS) de six populations comprenant des populations de basses et hautes altitudes, une étude précédente a identifié un sous-ensemble de 171 polymorphismes nucléotidiques (SNP candidats) présentant des signaux de sélection. Nous avons utilisé ces SNP candidats pour tester l'association entre la variation génotypique et phénotypique de 18 caractères. Notre panel d’association était constitué de 1663 plantes provenant de graines de 11 populations échantillonnées le long de deux gradients d’altitude. Il a été évalué deux années consécutives dans deux jardins communs. Nous avons contrôlé sa structure neutre en utilisant 18 marqueurs microsatellites. La variation phénotypique a révélé l’existence d'un syndrome altitudinal composé de dix caractères. Nous avons ainsi observé une augmentation de la précocité de floraison, une diminution de la production de talles et de la densité en stomates des feuilles ainsi qu’une augmentation de la taille, de la longueur et du poids des grains avec l’élévation croissante du site de collecte des populations. Ce syndrome a évolué malgré des flux de gènes détectables entre populations. Nous avons montré que le pourcentage de SNP candidats associés aux différents caractères dépend de la prise en compte de la structure neutre soit en cinq groupes génétiques (71,7%), soit en onze populations (11,5%), indiquant une stratification complexe. Nous avons testé les corrélations entre les variables environnementales et les fréquences alléliques des SNP candidats sur 28 populations. Nous avons trouvé un enrichissement à la fois pour les SNP présentant des associations phénotypiques et les SNP présentant des corrélations environnementales dans trois larges inversions chromosomiques, confirmant leur rôle dans l'adaptation locale. Pour explorer la contribution de la variation structurale à l'évolution adaptative, nous nous sommes concentrés sur le contenu en éléments transposables (ET) des six populations séquencées (HTS). Ces éléments constituent environ 85% du génome du maïs et contribuent à sa variabilité fonctionnelle. Nous avons effectué la première description populationnelle des ET chez les téosintes pour deux catégories d'insertions, celles présentes et celles absentes du génome de référence du maïs. Nous avons ensuite recherché des polymorphismes liés aux ET présentant des fréquences alléliques contrastées entre populations de basse et de haute altitude. Nous avons identifié un sous-ensemble d'insertions candidates. Enfin, nous avons génotypé, dans un panel d'association, des insertions d’ET connues pour avoir contribué à l'évolution phénotypique du maïs. Contrairement à ce qui a été observé chez le maïs, certaines de ces insertions n'ont montré aucun effet phénotypique chez les téosintes, ce qui suggère que leur effet dépend du fond génétique. Notre étude apporte de nouvelles connaissances sur l’adaptation altitudinale chez les plantes. Elle ouvre la discussion sur les défis soulevés par l'utilisation (1) d'outils de génomique des populations pour identifier la variation adaptative, (2) de populations naturelles en génétique d’association, et (1) de ressources génétiques sauvages pour l'amélioration des espèces cultivéesAnnual teosintes, the closest wild relatives of maize, are ideal systems to study local adaptation because their distribution spans a wide range of environmental conditions. Zea mays ssp. parviglumis is distributed in warm and mesic conditions below 1800 m, while Zea mays ssp. mexicana thrives in dry and cool conditions at higher altitudes. We combined reverse ecology and association mapping to mine the determinants of local adaptation in annual teosintes. Based on high throughput sequencing (HTS) data from six populations encompassing lowland and highland populations growing along two elevation gradients, a previous study has identified candidate regions displaying signals of selection. Within those regions a subset of 171 candidate single nucleotide polymorphisms (SNPs) was selected to test their association to phenotypic variation at 18 traits. Our association panel encompassed 1663 plants from seeds collected from eleven populations sampled along the elevation gradients. We benefit from phenotypic characterization of all the plants in two common gardens located at mid-altitude for two years. In addition, we controlled for neutral structure of the association panel using 18 microsatellite markers. Phenotypic variation revealed the components of an altitudinal “syndrome” constituted of ten traits evolving under spatially-varying selection. Plants flowered earlier, produced less tillers, displayed lower stomata density and carried larger, longer and heavier grains with increasing elevation of population collection site. This syndrome evolved in spite of detectable gene flow among populations. The percentage of candidate SNPs associated with traits largely depended on whether we corrected for five genetic groups (71.7%) or eleven populations (11.5%), thereby indicating a complex stratification in our association panel. We analyzed correlations between environmental variables and allele frequencies of candidate SNPs on a larger set of 28 populations. We found enrichment for SNPs displaying phenotypic associations and environmental correlations in three Mb-scale chromosomal inversions, confirming the role of these inversions in local adaptation. To further explore the contribution of structural variation to adaptive evolution, we focused on transposable element (TE) content of the HTS populations. TEs constitute ~85% of the maize genome and contribute to its functional variability via gene inactivation and modulation of gene expression. We performed the first population-level description of TEs in teosintes for two categories of insertions, those present and those absent from the maize reference genome. We next searched for TE polymorphisms with contrasted allele frequencies between lowland and highland populations. We pinpointed a subset of adaptive candidate insertions. Finally, we genotyped in our association panel TE insertions known to have contributed to maize phenotypic evolution. In contrast to what was found in maize, some of these insertions displayed no measurable phenotypic effects in teosintes, suggesting that their effect depends on the genetic background. Altogether our study brings new insights into plant altitudinal adaptation. It opens discussions on the challenges raised by the use (1) of population genomic tools to discover adaptive variation, (2) of natural populations in association mapping, and (1) of wild genetic resources in crop breeding
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Severinsson, Louise, and Maria Åkesson. "Samband och könsskillnader mellan kriminalvårdares personlighetsdrag och upplevelse av säkerhetsklimat." Thesis, Högskolan Väst, Avd för psykologi och organisationsstudier, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:hv:diva-3694.
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Anstalten Hall utanför Södertälje tillhör säkerhetsklass A, vilket innebär att de intagna utgör en hög risk för allmänheten. De anställda har därmed ett stort ansvar i att upprätthålla säkerheten på anstalten. Syftet med föreliggande studie var att undersöka möjliga samband mellan kriminalvårdarnas personlighet, deras upplevelse av säkerhetsklimatet och upplevda incidenter. Vidare studerades könsskillnader avseende personlighetsdrag och upplevelse av säkerhetsklimat. För att undersöka detta användes säkerhetsformuläret NOSACQ-50 och personlighetsskalan Swedish universities Scales of Personality (SSP). Totalt ingick 30 deltagare i studien. Signifikanta samband fanns mellan personlighetsdragen misstroende, bitterhet, somatisk ångestbenägenhet, social konformitet och verbal aggressionsförmåga i förhållande till hur de anställda upplevde säkerhetssystemet samt ledningen och kollegornas säkerhetsarbete. Signifikanta skillnader i personlighetsdragen fanns endast mellan mäns och kvinnors somatiska ångestbenägenhet, där kvinnor hade utmärkande högre värden. I övrigt visades inga signifikanta skillnader mellan personlighetsdragen. Vad det gällde upplevelser av säkerhetsklimatet på anstalten mellan könen kunde vissa skillnader utläsas, men dessa var inte signifikanta. Resultaten antyder att människor som arbetar inom kriminalvården är ganska lika varandra och att personlighetsdrag till viss del kan inverka på upplevelsen av arbetsplatsens säkerhetsklimat. Då antalet deltagare är begränsat bör dock slutsatser dras med försiktighet.The prison Hall near Södertälje has safety class A. This means that prisoners constitute a high risk to the public and the employees have a great responsibility in maintaining security at the prison. The purpose of this study was to explore possible relationships and differences between the prison officers’ views on safety climate and their personal traits. Furthermore gender differences in personality traits and experience of safety climate were studied. The security form NOSACQ-50 and Swedish universities Scales of Personality (SSP) were used. A total of 30 participants were included in the analysis. Significant correlations were found between the personality traits mistrust, embitterment, somatic trait anxiety, social desirability and verbal trait aggression in relation to how the employees experienced the safety climate. Significant differences in personality traits were found between male and female only in somatic trait anxiety, where women had distinctively higher values then men. When it came to perceptions of safety climate between the sexes some differences could be seen, but these were not significant. The results suggest that men and women working in prisons are quite similar and that personality traits may to some extent affect the experience of the workplace safety climate. As the number of participants is limited, however, care should be taken when making conclusions.
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Huber, Sieglinde Maria Franziska Elisabeth. "Isolierung und Charakterisierung des sporenspezifisch exprimierten Gens ssp1 in Ustilago maydis." [S.l. : s.n.], 2001. http://archiv.ub.uni-marburg.de/diss/z2002/0082/.
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Chowdhury, Tahmeena. "Interaction between the AAA+ protease CIpXP and the adaptor protein SspB." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/58198.
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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2010."June 2010." Cataloged from PDF version of thesis.
Includes bibliographical references (p. 96-102).
Proteolysis plays a vital role in cellular processes including regulatory pathways and protein quality control in prokaryotes and eukaryotes. ATP-dependent protein degradation is mediated by multimeric protease complexes, each consisting of a AAA+ ATPase and a peptidase component. Substrate selection by the proteases is a highly regulated process to ensure minimal errant protein degradation. Substrates are usually recognized by proteases through degradation tags or degrons. Accessory proteins called adaptors can also modulate substrate selection by proteases. These adaptors have the potential to affect substrate specificity as well as expand the repertoire of substrates that can be degraded by proteases. Understanding how proteases interact with a wide range of adaptors and substrates can provide valuable insight into the complex process of substrate selection. In this thesis, I have investigated the interaction between the AAA+ protease CIpXP and the adaptor protein SspB. The highly conserved N-terminal domain of the unfoldase CIpX interacts with SspB and other specific adaptor proteins and substrates. However, these binding partners do not use one simple sequence motif to mediate the protein-protein interaction. This diversity in protein-binding was further demonstrated by the cross-species CIpX-SspB interactions in Caulobacter crescentus and Escherichia coli. Despite little sequence homology, C. crescentus SspB (ccSspBa) and E. coli SspB (EcSspB) are able to interact with CIpX from either species. We analyzed these interactions to understand how the N-terminal domain of CIpX is able to recognize diverse adaptors and substrates while still retaining specificity. We identified the region important for interaction of ccSspBa with CIpX. Mutagenesis studies of the C-terminal region of the adaptor were conducted and the variants were tested for their ability to functionally interact with the CIpXP protease. Using these data and the results of peptide-binding experiments, we identified residues within the C-terminal region of ccSspBa that are important for tethering to CIpX. We also conducted functional and peptide-binding studies on the EcSspB CIpX-binding (XB) region. Interestingly, the two XB regions are very different in both length and sequence. However, despite this dissimilarity, competition studies argue that the two XB peptides bind to identical or overlapping sites of the CIpX N domains of C. crescentus and E. coli. This cross-species interaction between SspB and CIpX highlights how the CIpX N domain provides a versatile platform for binding a variety of adaptors and substrates. We also performed a proteomic-screen to investigate the effect of SspB on CIpXP substrate profile in E. coli. The preliminary data has provided a list of candidate SspB-interacting substrates, further analyses of which will contribute to the understanding of the biological impact of SspB on substrate selection by CIpXP.
by Tahmeena Chowdhury.
Ph.D.
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Byttner, Cajsa, and Tengvall Lisa Olsson. "Samverkan i form av SSPF : Är det rätt väg att gå?" Thesis, Uppsala universitet, Centrum för socialt arbete - CESAR, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-352509.
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I vår studie har vi undersökt hur samverkan inom verksamheten SSPF (samverkan mellan skola, socialtjänst, polis och fritid) i Uppsala kommun fungerar enligt aktörerna. Vi avsåg att undersöka aktörernas uppfattning av hur samverkan fungerar och ifall uppfattningen skiljde sig mellan de olika yrkesgrupperna. Fokus för uppsatsen var således inte att mäta effekterna av insatser som utförts via SSPF:s verksamhet. Studien har utgått från tidigare forskning om lyckad samverkan genom engagemang, lyhördhet, tillgänglighet och tillit, samt omständigheter som utgör hinder för samverkan såsom; bristande samsyn, oförståelse för andra aktörers uppdrag och skiljande regleringar. Detta, tillsammans med vårt teoretiska ramverk, gav en övergripande bild av forskningsfältet och verkade som underlag för analys och diskussion kring resultatet. Det teoretiska ramverket bestod av begrepp som gräsrotsbyråkrati, roller och handlingsutrymme som alla kopplas samman till begreppet samverkan. Det empiriska materialet bestod av data från vår genomförda enkätstudie om SSPF–samverkan i Uppsala kommun. Till den jämförande analysen genomfördes Chi 2 – tester. Utifrån resultatet kunde vi utläsa att aktörerna inom SSPF ansåg att samverkan hade bidragit till nytta i störst utsträckning i form av "Informationsutbyte", "Helhetssynen på ungdomar i riskfyllda situationer" och "Kontaktvägar". De förhållanden som aktörerna ansåg utgjorde hinder för SSPF-samverkan i störst utsträckning var "Bristande ekonomiska resurser", ""Krockande" lagstiftning och regelverk" och "Bristande personalresurser". Mellan yrkesgrupperna skola, socialtjänst och fritid förekom signifikanta skillnader för variabeln ""Krockande" lagstiftning eller regelverk” som hinder för SSPF-samverkan. För yrkesgrupperna skola och fritid fanns signifikanta skillnader för variablerna ”Bristande personalresurser” som hinder och ”Kontaktvägar” som nytta. Slutligen påvisades även signifikanta värden mellan yrkesgrupperna socialtjänst och fritid gällande ”Bristande/oklart beslutsmandat”.
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Shelton, Jennifer. "Epicuticular wax chemistry, morphology, and physiology in sand bluestem, andropogon gerardii ssp. hallii, and big bluestem, andropogon gerardii ssp. gerardii." Thesis, Kansas State University, 2012. http://hdl.handle.net/2097/14198.
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Master of ScienceDepartment of Biology
Loretta Johnson
Plant epicuticular wax (ECW) isolates internal tissues from harsh external conditions increasing drought tolerance. Beta-diketone-rich ECW reflect light and result in a glaucous phenotype that may ameliorate the thermal environment of the leaf. The overall goal is to characterize the form and function of ECW in two closely related, but phenotypically divergent grasses. [italicized]Andropogon gerardii ssp. [italicized]gerardii, big bluestem, is a non-glaucous, agronomically and ecologically dominant grass in the United States while [italicized]Andropogon gerardii ssp. [italicized]hallii, sand bluestem, is a glaucous subspecies restricted to dry, sandy soils. The objectives are to contrast sand and big bluestem ECW chemistry, morphology, and physiology to determine the distinctions in ECW resulting in the glaucous phenotype and determine the effect this has on leaf optical qualities and permeability. Gas chromatography mass spectrometry (GC-MS) and scanning electron microscopy (SEM) were used to examine ECW chemistry and micromorphology. It was hypothesized that beta-diketones and beta-diketone tubules where present only in leaves of sand bluestem and that the ECW was more reflective and abundant and the cuticle was less permeable. Beta-diketones and tubular ECW were absent in big bluestem and common on sand bluestem’s surface, although less than 20% of ECW was beta-diketones. Functional implications of ECW phenotypes were investigated by comparing minimum conductance (G[subscript]min), wax load, reflectance, and transmittance. Reflectance, with and without ECW, and G [subscript]min were measured with an infrared gas analyzer and a spectroradiometer, respectively. Sand bluestem had twice the ECW in mg cm[superscript]2 (P=.01) and three times lower G [subscript]min in ms[superscript]-1 10[superscript]-5 (P=.02). Partial least squares (PLS) models were trained to predict subspecies from reflectance spectra and were able to distinguish the subspecies. These experiments indicate that in comparison to big bluestem, increased reflectance is a property uniquely imparted to sand bluestem by ECW and the presence of beta-diketones determines the distinction. Glaucous crop species have shown higher yield under drought and extreme weather, including drought, is expected to become more common. Therefore, this study of glaucous waxes, may be applied in engineering drought tolerance.
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Elze, Julia. "Nachweis von Mycobacterium avium ssp. paratuberculosis bei Schlachtrindern." Hannover Bibliothek der Tierärztlichen Hochschule Hannover, 2010. http://d-nb.info/1000021831/34.
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Bandyopadhyay, Amrit. "The smart stone protocol (SSP) design and protocols /." College Park, Md. : University of Maryland, 2006. http://hdl.handle.net/1903/3953.
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Thesis (M.S.) -- University of Maryland, College Park, 2006.Thesis research directed by: Dept. of Electrical and Computer Engineering. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
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Purcell, Mark Douglas. "The mosquitocidal activity of Bacillus thuringiensis ssp. israelensis." Thesis, University of Cambridge, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624705.
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Hidalgo, Larsson Anna. "Forward Modelling of Ground Based SST Telescope Images." Thesis, KTH, Skolan för elektroteknik och datavetenskap (EECS), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-302729.
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Space debris is becoming an increased threat to the future use of space orbits. In order to counteract this threat, the field of Space Situational Awareness (SSA), and the sub-field Space Surveillance and Tracking (SST), have been developed to gather knowledge about the space debris and satellites surrounding Earth. The orbit of a satellite can be determined by acquiring images of the satellite using a telescope and a sensor. During this thesis, a tool has been programmed in Python. This tool can simulate these types of images of satellite passes, at a given time and location. The simulator takes the system parameters of the telescope and camera sensor into account, together with several different types of disturbances which affect these images. The project has been carried out at the Swedish Space Corporation (SSC), which recently launched an SSA initiative. They plan to use these images to learn more about their upcoming observations, and possibly to test an orbit determination software.Rymdskrot är ett allt mer påtagligt hot mot den framtida användningen av om-loppsbanor i rymden. För att motverka detta hot har det blivit viktigt att kartlägga rymdlägesbilden och de objekt som ligger i omloppsbana runt jorden. Detta görs genom att observera, identifiera och banbestämma satelliter. En satellits omlopps-bana kan bestämmas genom att ta bilder av satelliten med hjälp av ett teleskop och en sensor. Under detta examensarbete har ett verktyg för att kunna simulera sådana bilder utvecklats. Simuleringsverktyget har programmerats i Python och kan simulera bilder av satellitpass vid en given tidpunkt och från en given plats. Verktyget tar hänsyn till systemparametrarna för teleskopet och kamerasensorn, samt effekterna av ett flertal olika typer av störningar som påverkar dessa bilder. Projektet har genomförts hos företaget Swedish Space Corporation (SSC), som nyligen lanserade ett initiativ för att bättre förstå rymdlägesbilden. De planerar att använda dessa bilder för att lära sig mer om deras kommande observationer, samt att eventuellt testa en programvara för att bestämma banparametrar.
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NOGUEIRA, Ana Carolina Wanderley. "Caracterização de genes de resistência a patógenos em eucalipto (Eucalyptus ssp.), cana-de-açúcar (Saccharum ssp.) e feijão-caupi (Vigna unguiculata)." Universidade Federal de Pernambuco, 2007. https://repositorio.ufpe.br/handle/123456789/6372.
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Previous issue date: 2007Os genes de resistência (R) respondem pela primeira interação entre planta e patógeno, sendo responsáveis pela ativação ou não de mecanismos de resistência em plantas. Este trabalho analisou genes R em sequências expressas de eucalipto, cana-de-açúcar e feijão-caupi, geradas através de bibliotecas produzidas a partir de diferentes tecidos em várias fases de desenvolvimento. Depois da análise in silico foi possível a identificação de todas as classes de genes de resistência em eucalipto, com destaque para a classe NBS-LRR (Nucleotide Binding Site; Sítio de Ligação de Nucleotídeo - Leucine Rich Repeats; Repetições Ricas em Leucina) (50% das 208 sequências candidatas que apresentaram domínios completos) e em cana-de-açúcar, com destaque para a classe KINASE (46% das 196 sequências candidatas que apresentaram domínios completos). No feijão-caupi o número de seqüências disponíveis foi escasso, observando-se maior abundância da classe NBS-LRR (80% das 38 sequências candidatas), entretanto estiveram ausentes as classes KINASE e LRR-KINASE. Observaram-se genes R em cana e eucalipto em todos os tecidos analisados, em diferentes níveis de expressão sob condições não induzidas. Quando analisados através de alinhamentos múltiplos os genes R apresentaram maior semelhança entre espécies pertencentes à mesma família, geralmente agrupando mono e dicotiledôneas em clados distintos, sugerindo que tenham surgido antes da separação entre essas classes. Os resultados do presente estudo têm potencial para colaborar com o desenvolvimento de marcadores moleculares para o melhoramento, para o entendimento da abundância e diversidade e evolução destes genes, com ênfase das espécies estudadas, bem como para identificação dos genes R em outras culturas de interesse econômico
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Rodriguez, Jacqueline. "Livet som missbrukare och kriminell : – Betydelsen av avvikande personlighet och mönster i livshistoria." Thesis, Stockholms universitet, Psykologiska institutionen, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-113647.
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Frågorna kring orsaken till varför en ”väljer” ett liv som kriminell och missbrukare har inga enkla svar. Syftet med föreliggande undersökning var att söka ökad förståelse av kriminalitet och missbruk. Detta gjordes genom att göra en jämförelse mellan undersökningsdeltagarnas reflektioner rörande sina tidigare livsstilar som kriminella och missbrukare och resultaten från deras personlighetstest. I studien deltog 5 personer i åldrarna 24-61. Via semistrukturerade intervjuer och tematisk analys har deltagarnas upplevelser och reflektioner kring deras tidigare livsstilar beskrivits vilket besvarar studiens ena frågeställning. Med hjälp av Swedish Universities Scales of Personality (SSP) kunde en personlighetsprofil skapas för varje deltagare. Vad som har utkommit från varje enskild berättelse relaterades sedan till SSP resultaten, det besvarade andra studiens frågeställning. Inom extraversionsfaktorn utmärktes två värden avvikande från normen för samtliga deltagare, interpersonell distans och impulsivitet. Avvikande värden för interpersonell distans har märkts hos 4 av 5 deltagare. Impulsivitet, enligt SSP resultat, har funnits hos 3 av 5 deltagare.
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Valdivia, Zegarra William. "Propuesta de implementación de un Sistema de Gestión de Seguridad y Salud Minera en el trabajo, para la Empresa M3 Ingeniería Perú S.A.C." Bachelor's thesis, Universidad Continental, 2018. http://repositorio.continental.edu.pe/handle/continental/5206.
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Se determinó una deficiencia en el SG- SS incumpliendo la normativa legal actual del País. Se refleja en un ámbito de trabajo de situaciones inseguras, donde se pone en riesgo la integridad de su personal laboral ya que están sujetos a los riesgos que se presentan en su centro laboral”, así mismo su recomendación más relevante es que son “Dos aspectos importantes a considerar para conseguir el éxito de la realización del SG - SST son, el comprometerse e involucrase por la alta dirección y la participación dinámica de todos sus trabajadores en cada fase del sistema .
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Mohamed, Abdul Halim. "The impact of the Malaysian Smart Schools' Training Programme (SSTP) : teachers' perspectives." Thesis, University of East Anglia, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.429817.
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Feng, Xiaohu. "SIC BASED SOLID STATE POWER CONTROLLER." UKnowledge, 2007. http://uknowledge.uky.edu/gradschool_diss/543.
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The latest generation of fighter aircraft utilizes a 270Vdc power system [1]. Such high voltage DC power systems are difficult to protect with conventional circuit breakers because the current does not automatically go to zero twice per cycle during a fault like it does in an AC power system and thus arcing of the contacts is a problem. Solid state power controllers (SSPCs) are the solid state equivalent of a circuit breaker that do not arc and which can respond more rapidly to a fault than a mechanical breaker [2]. Present SSPCs are limited to lower voltages and currents by the available power semiconductors [8,9]. This dissertation presents design and experimental results for a SSPC that utilizes SiC power JFETs for the SSPC power switch to extend SSPC capability to higher voltages and currents in a space that is smaller than what is practically achievable with a Si power switch. The research started with the thermal analysis of the SSPCs power switch, which will guide the development of a SiC JFET multi-chip power module to be fabricated by Solid State Devices Inc. (SSDI) using JFETs from SiCED and/or Semisouth LLC. Multiple multi-chip power modules will be paralleled to make the SSPC switch. Fabricated devices were evaluated thermally both statically and dynamically and electrically both statically and dynamically. In addition to the SiC module research a detailed design of the high voltage SSPC control circuit capable of operating at 200andamp;ordm;C was completed including detailed analysis, modeling and simulations, detailed schematic diagrams and detailed drawings. Finally breadboards of selected control circuits were fabricated and tested to verify simulation results. Methods for testing SiC JFET devices under transient thermal conditions unique to the SSPC application was also developed.
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Liu, Chunli. "THE ROLE OF POLYAMINE ACETYLATION IN REGULATING ADIPOSE TISSUE METABOLISM." Diss., Temple University Libraries, 2011. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/213123.
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BiochemistryPh.D.
Because excessive body weight is a major health issue, there is an urgent need to understand all physiological mechanisms relating to control of fat deposition/mobilization. Here we investigated the linkage between polyamine metabolism and fat homeostasis that we recently discovered to operate in mice. Our previous data show that the expression level of spermine/spermidine acetyltransferase (SSAT), a polyamine catabolic enzyme, potently modulates body fat content of mice. In particular, our data indicated that SSAT overexpressing mice (SSAT-Tg) have reduced acetyl CoA levels and are lean while SSAT null mice (SSAT-ko) are obese. Since the acetyl CoA/malonyl CoA levels are critical for control of free fatty acid synthesis and oxidation via malonyl CoA regulation of CPT-1 (carnitine palmitoyltransferase-1), we hypothesized that genetic manipulation of SSAT alters body fat accumulation by activating of AMP-activated protein kinase pathway and thus has a global effect on energy metabolism. To test this hypothesis, we performed a combination of proteomics and antibody based expression studies in white adipose tissue (WAT) of SSAT-ko, SSAT-wt and SSAT-tg: We identified 9 proteins in WAT that show an increasing gradient across SSAT-ko, SSAT-wt and SSAT-tg, all of which have a connection with acetyl-CoA consumption. These include: a) glycolytic enzymes (aldolase, enolase, pyruvate dehydrogenase); b) TCA cycle enzymes (aconitate hydratase, malate dehydrogenase); c) fatty acid lipolysis and beta oxidation enzymes (hormone-sensitive lipase, monoglyceride lipase, 3-hydroxyacyl CoA dehydrogenase). Additional expression studies using Western blots indicated that acetyl CoA regulates metabolism by AMP-activated protein kinase pathway. Furthermore, to determine how tissue-specific changes in SSAT expression will impact fat accumulation and the precise role of SSAT expression status in fat homeostasis and obesity, we generated adipose-specific SAT1 knockout (FSAT1KO) mice using the Cre-Lox method. On 27-week-old, FSAT1KO mice have higher average body weight than wild type mice (WT: 45.13 ± 2.23 g vs. FSAT1KO: 52.28 ± 1.62 g, p<0.05) when fed a high-fat diet. Larger lipid droplets and lipid accumulation were present in FSAT1KO mouse livers compared to the control WT mice. Several proteins involved in fat metabolism were found to be up-regulated in FSAT1KO mice using GeLC-MS proteomics approach. These data indicated that the lack of SSAT activity in adipose tissue, but not liver or muscle, drives the phenotypic changes in SSAT-ko obese mice. Our interpretation of these results is that genetic modulation of SSAT causes a combination of changes in WAT that involve lipolysis, energy metabolism and calorie loss resulting from polyamine export. In summary, the data indicate that modulation of SSAT activity affects fat metabolism and calorie balance.
Temple University--Theses
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Lorentz, Lothar [Verfasser]. "Herbicide Resistance : Molecular and Physiological Characterization of the Glyphosate Resistant Weeds Amaranthus ssp. and Sorghum ssp. / Lothar Lorentz." Bonn : Universitäts- und Landesbibliothek Bonn, 2014. http://d-nb.info/1047666596/34.
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NABIL, MOHAMED. "Etudes des mecanismes de tolerance au chlorure de sodium et a une contrainte hydrique chez acacia nilotica ssp. Cupressiformis et ssp. Tomentosa." Clermont-Ferrand 2, 1995. http://www.theses.fr/1995CLF21687.
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Cette etude a porte sur l'analyse des effets du chlorure de sodium et d'une contrainte hydrique chez 2 sous-especes d'acacia nilotica. Au niveau de la germination, les graines d'acacia nilotica sont tolerantes a des concentrations en nacl atteignant les 200 mm avec seulement 50% de reduction de celle-ci. La croissance des plantes et les reponses physiologiques de 2 ssp. D'acacia nilotica (ssp. Cupressiformis (cu) et ssp. Tomentosa (to) ont egalement ete analysees. Apres un mois de stress: - salin (0,75, 100 et 200 mm en nacl) ; les plantes presentent une croissance active jusqu'a 100 mm ; elles survivent difficilement a 200 mm. La photosynthese, la conductance stomatique et la transpiration diminuent de facon tres importante. Les potentiels hydriques foliaires et osmotiques baissent avec la salinite. Les diagrammes de hofler confirment que l'ajustement osmotique a eu lieu sous tous les traitements. De plus, une hausse du module d'elasticite a ete observee en conditions de stress. L'ajustement osmotique et les modules d'elasticite eleves modifient la capacite des 2 ssp. A maintenir une balance hydrique positive. L'accumulation en ions (na#+, k#+ et cl#-) et en proline pourrait expliquer un tel ajustement. Acacia nilotica ssp. Cupressiformis presente une meilleure absorption de k#+ que tomentosa au-dela de 100 mm nacl. - hydrique ; les activites rubisco sont peu affectees par le stress. L'ajustement osmotique observe est du a une accumulation en sucres et en cl#-. Le stress ne modifie pas la teneur en proline dans les feuilles alors que sa concentration est multipliee par 4 dans les racines. Face a la salinite et a la deshydratation, les ssp. Etudiees adoptent 2 strategies: - l'evitement pour le maintien d'une teneur en eau relative elevee par diminution de la conductance osmotique, - la tolerance en supportant des potentiels hydriques bas par des modules d'elasticite eleves tout en maintenant la turgescence des cellules par diminution du potentiel osmotique
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Gilbert, Philippe-Alexandre. "Expression de la bioluminescence chez Lactococcus lactis ssp. cremoris." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq25592.pdf.
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Kang, Ok-Ju. "Isolement, localisation et caractérisation des autolysines chez Lactobacillus ssp." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ31497.pdf.
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Bayindir, Ürün [Verfasser]. "HINOKININ BIOSYNTHESIS IN Linum strictum ssp. corymbulosum / Ürün Bayindir." Aachen : Shaker, 2008. http://d-nb.info/1164341871/34.
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Landeen, Melissa L. "Mountain Big Sagebrush (Artemisia tridentata ssp vaseyana) Seed Production." BYU ScholarsArchive, 2015. https://scholarsarchive.byu.edu/etd/5740.
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Big sagebrush (Artemisia tridentata Nutt.) is the most widespread and common shrub in the sagebrush biome of western North America. Of the three most common subspecies of big sagebrush (Artemisia tridentata), mountain big sagebrush (ssp. vaseyana; MBS) is the most resilient to disturbance, but still requires favorable climactic conditions and a viable post-fire seedbank for successful unassisted recovery. This study was designed to assess MBS seed production throughout post-fire recovery. We performed 2 pilot studies to develop methods for estimating seed production and plant age. The results of the pilot studies and a space-for-time substitution strategy were used to measure seed production on 13 sites ranging from 10-33 years post-fire. We hypothesized that seed rain (mean seeds produced/ m2) would peak before stand density had maximized due to decreasing individual plant fecundity (mean seeds produced/ plant) in high density stands. We measured population density and individual plant fecundity for three size classes of MBS and used forward stepwise regression analysis to identify environmental factors influencing seed production over time. Density for small (basal stem diameter (BSD) <1 cm) and medium-sized (BSD=1-3 cm) plants was consistently low and was not affected by time since fire (TSF), while large-sized (BSD > 3 cm) plant density increased steadily with TSF (p=0.0002). Plant fecundity decreased with TSF for all three size classes (p range = 0.019 – 0.0506), with large plants dominating reproductive output. Small and medium-sized plant fecundity was negatively correlated with winter precipitation (p range = 0.0106-0.0174), while large plant fecundity was positively correlated with winter precipitation (p<0.0001) and negatively correlated with elevation (p=0.0001). Despite losses in plant fecundity over time for all size classes, steady recruitment in population density resulted in increased seed rain (p=0.0039), suggesting that increases in stand density compensated for losses in individual plant fecundity. Results partially support our hypothesis that the time required for MBS seed rain to be maximized was not tightly bound to indicators of stand maturation. Understanding the factors that influence post-fire seed production can help land managers better manage for successful recovery by providing them with tools for evaluating seed production capabilities of MBS communities.
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Chagas, Júnior Adenizar Delgado das. "O imprint como método para detecção de Leptospira SSP." s.n, 2009. https://www.arca.fiocruz.br/handle/icict/4113.
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Adenizar Chagas Júnior. O Imprint como método para detecção de Leptospira SSP. 2009.pdf: 396164 bytes, checksum: 8f423288d4f795e861df22e9de3acb59 (MD5)Made available in DSpace on 2012-05-31T19:55:30Z (GMT). No. of bitstreams: 1 Adenizar Chagas Júnior. O Imprint como método para detecção de Leptospira SSP. 2009.pdf: 396164 bytes, checksum: 8f423288d4f795e861df22e9de3acb59 (MD5) Previous issue date: 2009
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Na determinação da eficácia de novas candidatas à vacina para leptospirose, o marcador primário considerado é a mortalidade, e um marcador secundário importante é a indução de uma imunidade estéril. Entretanto, a avaliação da imunidade estéril é dificultada pelo tempo demandado e pela complexidade de métodos como o isolamento pela cultura. Neste estudo, foi avaliado o uso do método do imprint (ou touch preparation) na detecção da presença de leptospiras em tecidos de hamsters infectados com L. interrogans sorovar Copenhageni. Comparado com a cultura, o imprint demonstrou igual ou melhor detecção de leptospiras em amostras de rim, fígado, pulmão e sangue coletadas após a infecção obtendo uma concordância geral boa (κ = 0.61). Além disso, na avaliação de hamsters imunizados com uma proteína recombinante de Leptospira candidata à vacina e subsequente desafio com leptospiras patogênicas, a concordância entre a cultura e o imprint foi alta (κ = 0.84). Estes achados indicam que o imprint é um método rápido para a observação direta de Leptospira spp. e que pode ser facilmente aplicado na avaliação de animais infectados experimentalmente com leptospiras e na determinação de imunidade esterilizante durante avaliações de potenciais candidatas à vacina.
In determining the efficacy of new vaccine candidates for leptospirosis the primary endpoint is death and an important secondary endpoint is sterilizing immunity. However, evaluation of this endpoint is often hampered by the time consuming demands and complexity of methods such as culture isolation (CI). In this study, we evaluated the use of an imprint (or touch preparation) method (IM) in detecting the presence of leptospires in tissues of hamsters infected with L. interrogans serovar Copenhageni. Compared to CI, the IM exhibited equal or improved detection of leptospires in kidney, liver, lung and blood samples collected post-infection and the overall concordance was good (κ = 0.61). Furthermore, in an evaluation of hamsters immunized with a recombinant Leptospira protein-based vaccine candidate and subsequently challenged with leptospires, the agreement between the CI and IM was very good (κ = 0.84). These finding indicate that the IM is a rapid method for the direct observation of Leptospira spp. that can be readily applied to evaluating Leptospira infection in experimental animals and determining sterilizing immunity when screening potential vaccine candidates.
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Stein, Katrin [Verfasser], and Rüdiger [Akademischer Betreuer] Korbel. "Entwicklung einer Realtime-PCR zum Nachweis von Mycobacterium avium ssp. avium und Mycobacterium avium ssp. silvaticum beim Vogel / Katrin Stein. Betreuer: Rüdiger Korbel." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2011. http://d-nb.info/1017233063/34.
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Babbar, Naveen. "Regulation and function of spermidine/spermine N¹ acetyl transferase (SSAT) in colon carcinogenesis." Diss., The University of Arizona, 2003. http://hdl.handle.net/10150/289966.
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Epidemiological data suggest that non-steroidal anti-inflammatory drugs (NSAIDs) have anti-tumorigenic activities against colorectal cancer. NSAIDs work by inhibiting cyclooxygenases (COX) enzyme. Sulindac, a NSAID prodrug, is metabolized into pharmacologically active sulfide and sulfone derivatives. Microarray analysis was used to detect COX independent effects of sulindac on gene expression in human colorectal cells. Spermidine/spermine N 1-acetyl transferase (SSAT) gene, which encodes a polyamine catabolic enzyme, was one of the genes induced by clinically relevant sulindac sulfone concentrations. Promoter analysis and mutational studies were done to map the sulindac sulfone dependent response sequences in SSAT 5' flanking sequences, which led to the identification of two Peroxisome Proliferator Activated Receptors (PPARs) response elements (PPREs) in the SSAT gene. PPRE-2 is required for the induction of SSAT by sulindac sulfone and is specifically bound by PPARgamma in the Caco-2 cells, while PPRE-1 is not required for the induction of SSAT by sulindac sulfone, but can be bound by both PPARdelta and PPARgamma. Clinically relevant concentrations of sulfone reduced intracellular polyamine levels, inhibited cell growth and induced apoptosis in colon cancer cells. Further, only sulindac sulfone induced apoptosis could be partially rescued by exogenous polyamines. Upon evaluating other NSAIDs for their action on SSAT gene expression, it was found that they induce SSAT mRNA in either a COX dependent or independent mechanism in colon cancer cells. Studies with physiologically relevant concentrations of aspirin show that these concentrations can induce SSAT expression thereby leading to a decrease in polyamine levels. Activating mutations in K-ras, which is a late process in colon carcinogenesis, led to the suppression of SSAT expression in the Caco-2 cells due to the inhibition of PPARgamma by ERK. K-ras didn't have any effect on the induction of SSAT by sulindac sulfone but partially abolished the apoptosis caused by sulindac sulfone, indicating a possible role of mutant K-ras in sulindac resistant colon polyps. Sulindac sulfone, or Exisulin(TM) have been recently used in clinical trials for the prevention of colon, lung and prostate cancer. The data shown here, suggest that one of the mechanisms, by which sulindac sulfone could act as a chemopreventive agent is to induce the expression of SSAT thereby leading to a decrease in the intracellular polyamines. This reduction in polyamines plays an important part in the apoptosis induced by sulindac sulfone in the colon cancer cells. Further, induction of SSAT seems to a general mechanism for different NSAIDs like aspirin, indomethacin, ibuprofen, sulindac and celecoxib in colon cancer. Aspirin is able to induce SSAT and decrease intracellular polyamines at physiological concentrations, which can lead to a significant reduction in adenoma recurrence. Also, activated K- ras suppressed SSAT, but was not able to abolish the induction of SSAT by sulindac sulfone indicating the potential of using sulindac sulfone in colon cancer chemoprevention.
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Hosseini, Ramin. "Single strand-preferring nuclease (SSPN) activity and ultrastructural changes in ageing rice cells." Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285570.
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Moraes, Helen Pimenta de [UNESP]. "Avaliação in vitro da atividade antibacteriana de extratos de Byrsonima ssp E Alchornea ssp: estudo comparativo entre as técnicas de diluição em tubos e microplacas." Universidade Estadual Paulista (UNESP), 2006. http://hdl.handle.net/11449/97904.
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moraes_hp_me_arafcf.pdf: 1421590 bytes, checksum: 13ffb8dfe23f8d17e358dcedf26cd6c1 (MD5)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Universidade Estadual Paulista (UNESP)
O Brasil apresenta uma grande diversidade de plantas medicinais, cuja utilização pela população vem aumentando cada vez mais. Byrsonima (Malpighiaceae) e Alchornea (Euphorbiaceae) que são plantas do Bioma-Cerrado do estado de São Paulo apresentam compostos com grande potencial de atividade biológica, sendo entretanto, pouco estudadas. A atividade antimicrobiana é uma etapa de extrema importância na caracterização biológica de produtos naturais com potencial farmacológico. Nossos objetivos foram investigar a atividade antibacteriana de extratos metanólicos e clorofórmicos de Byrsonima spp. e Alchornea spp empregando as técnicas de diluição em tubos e em microplacas com leitura espectrofotométrica e visual utilizando resazurina como revelador. Foram utilizadas as bactérias: Escherechia coli, Staphylococcus aureus, Yersinia enterocolitica e Aeromonas hydrophila. Nos ensaios em microplacas e em tubos foi determinada a concentração inibitória mínima (CIM) dos extratos vegetais testados à concentração de 1000æg/mL a 31,25æg/mL. Objetivou-se também comparar a eficácia das metodologias empregadas. Nosso estudo demonstrou maior atividade antibacteriana dos extratos metanólicos tanto de Byrsonima quanto de Alchornea, ressaltando os dados obtidos com B. crassa (MeOH) e B. basiloba (MeOH) que apresentaram acentuada atividade antibacteriana frente aos diferentes microrganismos testados. As metodologias empregadas foram eficazes para essa finalidade sendo as de diluição em microplacas mais vantajosas, pelo emprego de pequenos volumes principalmente os de extratos vegetais que são obtidos em pequenas quantidades. Dentre os dois métodos de microdiluição, a que empregava leitura espectrofotométrica apresentou-se mais sensível, porém a de leitura visual mostrou-se mais prática na leitura final e na interpretação dos resultados.
Brazil presents a great diversity of medicinal plants, whose use by people increases more and more. Byrsonima (Malpighiaceae) and Alchornea (Euphorbiaceae) are plants of Bioma-Cerrado of the state of São Paulo and present composites with great potential of biological activity, but, however, they are little studied. The antimicrobial activity is a stage of extreme importance in the biological characterization of natural products with pharmacological potential. Our objectives were to investigate the antibacterial activity of methanolic and chlorophormic extracts of Byrsonima spp and Alchornea spp using the techniques of dilution in tubes and in microplates with spectrophotometric and visual readings using resazurin. Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica and Aeromonas hydrophila were tested. In the microplate and tubes assays, the minimum innhibitory concentration (MIC) of plant extracts were tested in concentrations between 1000æg/mL and 31.25æg/mL. It was also compared the effectiveness of the methodologies employed. Our study demonstrated greater antibacterial activity of methanolic extracts of both Byrsonima and Alchornea, standing out the data obtained for B. crassa (MeOH) and B. basiloba (MeOH), which presented accented antibacterial activity against the different tested microrganisms. The methodologies employed were efficient for this purpose and the microplate dilution assay is more advantageous, for the fact it uses smaller volumes, mainly for those vegetal extracts that normally are obtained in small amounts. Amongst the two methods of microdilution, the one that used spectrophotometric reading proved to be more sensitive, however, the visual reading procedure was shown to be more practical in the final reading and interpretation of the results.
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Khatibnia, Payam, and Jakob Toivonen. "Vägen till bankens mobilapp : En studie i storbankernas implementeringsarbete av självbetjäningsteknik." Thesis, Högskolan i Gävle, Avdelningen för ekonomi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-16436.
Full textAbstract:
Titel: Vägen till bankens mobilapp – En studie i storbankernas implementeringsarbete Syfte: Syftet med denna studie är att analysera hur svenska storbanker implementerar sin mobil självbetjäningsteknik (SST) bland konsumenter. Studien ska bidra till att skapa förståelse för hur organisationer arbetar med att implementera SST. Frågeställning: Hur har implementeringsmoment använts vid bankernas implementering av sin mobila SST? Är någon eller några av momenten mer vanligt förekommande i implementeringsarbetet än andra? Metod: Studien är baserad på vetenskaplig teori inom fältet för marknadsföring. Det teoretiska materialet består av vetenskapliga artiklar som behandlar implementering av SST. Genom att utgå från studiens syfte var det lämpligt att använda en kvalitativ metod. Studiens empiriska undersökning utgjordes av en fallstudie. Där intervjuades 10 stycken tjänstemän från tre av Sveriges storbanker. Genom att observera utfallet från intervjuerna erhölls nyckelord. Förekomsten av dessa nyckelord har sedan analyserats för att finna mönster i respondenternas svar. Resultat och Slutsats: Studien finner sju delmoment som är överförbara till implementeringsarbetet hos de banker som studerats. Studien kan inte visa huruvida någon eller några av momenten ska vara mer framträdande än andra i implementeringsarbetet. Slutligen visar studien en bild av hur de sju momenten för implementering av SST används hos tre av storbankerna på den svenska marknaden. Bidrag: Studien bidrar till forskningsområden inom SST genom att visa hur moment i implementeringsarbetet används av en organisation. För verksamheter och dess ledning bidrar studien till att visa hur implementeringsmomenten hanteras hos tre av de största bankerna inom den svenska marknaden. Studien bidrar även till att visa hur SST kan utgöra ett verktyg till att möta kunder i en flerkanalslösning. Begränsningar och förslag till framtida forskning: Att studien innefattar intervjuer hos 10 stycken respondenter kan påverka dess generaliserbarhet. Resultatet förväntas därför inte generaliseras till enskilda banker utan snarare bidra med en bild av bankers sätt att implementera SST. Vidare är studiens mönster nödvändigtvis inte överensstämmande med bankernas hela implementeringsarbete. Detta då vissa nyckelord kan användas trots att de inte innefattats i intervjuerna. Till fortsatt forskning rekommenderas att studera samma fenomen men mot verksamheter i andra branscher. Detta för att se huruvida implementerings-momenten även är applicerbara mot deras marknad. Ett ytterligare intressant ämne kan vara att undersöka hur kunder ser på implementeringsmomenten. Detta för att se om vissa moment anses mer viktiga för kundens adoption av SST.