To see the other types of publications on this topic, follow the link: SSHS.
Dissertations / Theses on the topic 'SSHS'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'SSHS.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
1
Veselovský, Matej. "Vytvoření monitorovacího a profilovacího řešení nad BI systémem." Master's thesis, Vysoké učení technické v Brně. Fakulta podnikatelská, 2017. http://www.nusl.cz/ntk/nusl-318361.
Full textAbstract:
This master’s thesis focuses on monitoring and profiling multidimensional database and reports. The project was created in MS SSIS to this purpose and it contains 4 ETL packages. The thesis is divided into three main sections. First section consists of theoretical background needed to accomplish the goal. In second section there is analysis of the company for which is the solution created and in the third part of the thesis there is described the proposal and it’s solution. Proposal of the solution and the solution itself was created in MS Visual Studio 2015, MS SQL Management Studio and Power BI.
2
Lundstedt, Mattias, and Axel Norell. "Prototyp för dynamiskt beslutsstöd." Thesis, Linköpings universitet, Interaktiva och kognitiva system, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-108954.
Full textAbstract:
Företaget Nethouse har haft uppdraget att kravställa, utveckla och implementera ett verksamhetssystem åt Sveriges Skorstensfejaremästares Riksförbund (SSR). Medlemsföretagen i SSR bedriver sotarverksamhet på uppdrag av Sveriges kommuner och är beroende av insamlad data kopplad till deras verksamhet. I det nyutvecklade systemet, som går under namnet Ritz, samlas informationen i en central databas och är tillgänglig för flertalet intressenter med hjälp av ny teknik och modernare lösningar. Systemet är helt webbaserat och körs som en molntjänst, tillgängligt via antingen en webbsida eller som mobilapplikation. Åtkomsten av data baseras på företagsnivå på ”stämplad” data i databasen och för att reglera åtkomsten för företagsanvändare till respektive företags data används rollbaserad åtkomstkontroll. Detta examensarbete har syftat till att utveckla en prototyp till en beslutsstödslösning för dynamisk åtkomst till de datamängder som lagras inom Ritz. Nethouse har efterfrågat en prototyp för en BI-lösning som visar på möjligheter och fördelar för intressenter till Ritz med att implementera en sådan. Då integration och förvaltning är viktiga faktorer för Nethouse har ett krav på prototypen varit att den utvecklats inom Microsofts programvaror, precis som resten av Ritz. Prototypen färdigställdes genom konstruerandet av ett centralt data warehouse enligt Ralph Kimballs metodologier och genom implementation av en OLAP-kub byggd i Microsoft SSAS. Dataöverföringen från datakällorna till beslutsstödslösningens data warehouse skedde genom utvecklandet av en ETL-process i Microsoft SSIS. Den resulterande kuben har främst utformats för att kunna besvara den sortens frågor som länsstyrelser ställer till sotarföretag i kontrollsyfte och stöder förfrågningar mot de två centrala affärsprocesserna sotning och brandskyddskontroll. Dessa förfrågningar kan filtreras på flertalet dimensioner som exempelvis tid, utförare, status och kontrollutfall. Prototypen begränsar även åtkomst till den information som användare har rätt att ta del av genom att koppla samman användare och objekt till geografiska indelningar som kallas distrikt. Denna dynamiska säkerhetslösning ger goda förutsättningar för att kunna hantera förändringar i användarnas behörighet i framtiden. Genom den utvalda lösningen behålls den dynamiska naturen i systemet, då åtkomst till beslutsstödstjänsten kan fås genom flertalet källor som stödjer uppkoppling mot Microsofts multidimensionella beslutsstödslösningar, bland annat Excel och SQL Server Reporting Services.
3
石川, 久美. "SSHオープンクラス." 名古屋大学教育学部附属中・高等学校, 2013. http://hdl.handle.net/2237/19153.
Full text
4
Collett, Torstein Calvin. "Simple SSH Management." BYU ScholarsArchive, 2021. https://scholarsarchive.byu.edu/etd/9130.
Full textAbstract:
SSH certificates are used by administrators so connections to the server can be verified. This ensures that only authorized administrators can access the server and that the server being accessed is the intended machine. Current solutions for managing SSH certificates are focused on commercial use, which makes them cumbersome for small groups and individuals to use. These solutions require running multiple services that companies already use but add significant overhead for smaller groups. We developed a new standalone system that makes it easy to manage SSH certificates for small amounts of servers and users, without requiring additional servers to be deployed. We evaluated our system with a user study to demonstrate its ease of use. We hope that this implementation can help guide future research toward a more simplified certificate authentication system for SSH.
5
Carlsson, Jacob, and Edison Gashi. "SSDs påverkan på MySQL: En prestandajämförelse." Thesis, Linnéuniversitetet, Institutionen för datavetenskap, fysik och matematik, DFM, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-21266.
Full textAbstract:
Solid State Drives (SSD) blir idag allt vanligare som lagringsmedium och håller på att bli ett alternativ till magnetiska hårddiskar. Denna studie+ har undersökt hur man på bästa sätt kan utnjytta SSDer i en MySQL-databas. Undersökningen genomfördes med hjälp av experiment där prestandamätningar gjordes för att få en klar bild på i vilken konfiguration av SSDs som ger bäst prestanda i MySQL. Mätverktygen som användes var sql-bench och mysqlslap. Resultaten visar att en databas på en enskild SSD presterar lika bra som en databas med SSD-cache under majoriteten av mätningarna och visar bättre resultat än resterande konfigurationerna som var en databas på hårddisk och transaktionsloggen på en SSD.Solid State Drives (SSD) are now becoming more common as storage and is about to become an alternative to magnetic disks. This report studied how to best utilize SSDs in a MySQL database. This study was carried out using experiments in which performance benchmarks were made to get an accurate view on which configuration of SSDs that gives the best performance in MySQL. The benchmarks where made with sql-bench and mysqlslap. The results indicate that a database using only SSD storage performs equal to a database of solid-state cache under the majority of the tests and shows better results than the remaining configurations that include a database on a single hard drive and a configuration with the transaction log on a SSD.
6
Roberto, Miguel Augusto Inácio. "Smart & Simple Business Solutions (SSBS)." Master's thesis, NSBE - UNL, 2010. http://hdl.handle.net/10362/10319.
Full text
7
Homaidi, Omar Al. "Data Remanence : Secure Deletion of Data in SSDs." Thesis, Blekinge Tekniska Högskola, Sektionen för datavetenskap och kommunikation, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-5165.
Full textAbstract:
The ongoing fast pace research in hardware and software technology has resulted in memory devices efficient and faster than ever before. However, the issue of security of the contained data is rarely discussed. There is an evident capability of these devices to retain data even when it is erased. In this thesis, a study is conducted to qualitatively analyze the extent to which data deletion is important and why secure deletion should be applied. Afterwards, following the sequential exploratory procedure, this paper presents an analysis of methods used to recover the data after being deleted in addition to the techniques used to securely delete this data. Based on this study, some recommendations are made to ensure the safety of data.
8
Матусяк, І. В., and Р. О. Козак. "Види атак на протокол SSH." Thesis, КНТУ, 2016. http://dspace.kntu.kr.ua/jspui/handle/123456789/5025.
Full text
9
Madron, Lukáš. "Datové sklady a OLAP v prostředí MS SQL Serveru." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2008. http://www.nusl.cz/ntk/nusl-235916.
Full textAbstract:
This paper deals with data warehouses and OLAP. These technologies are defined and described here. Then an introduction of the architecture of product MS SQL Server and its tools for work with data warehouses and OLAP folow. The knowledge gained is used for creation of sample application.
10
[記載なし]. "第5章 SSH通信 SSH通信 (第8号 9.30.2008)(Ⅷ. 資料)." 名古屋大学教育学部附属中学校 : 名古屋大学教育学部附属高等学校, 2010. http://hdl.handle.net/2237/12997.
Full text
11
Bijanskienė, Simona. "Kolektyvinės tapatybės formavimas SSRS ir ES: ekonominio veiksnio įtaka." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2008. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2008~D_20080610_140515-88047.
Full textAbstract:
Šio magistrinio darbo pavadinimas „Kolektyvinės tapatybės formavimas SSRS ir ES: ekonominio veiksnio įtaka“. Istorinės ribos apima laikotarpį nuo SSRS susikūrimo iki šių dienų aktualijų, žodis "formavimas" nurodo analizuojamo proceso t��stinumą. Pagrindinė darbo analizės ašis – kolektyvinė tapatybė.
Darbo pagrindinis tikslas yra įvertinti ES kolektyvinio tapatumo formavimo perspektyvumą, remiantis Sovietų Sąjungos patirtimi ir nustatyti ekonominio veiksnio ryšį su kolektyvinio tapatumo formavimo procesu. Siekiant užsibrėžtų tikslų, reikia atlikti šiuos išsikeltus uždavinius: visų pirma, reikia išsiaiškinti kaip yra konstruojamas kolektyvinis tapatumas, kokie veiksniai tai lemia ir kokia yra ekonominio veiksnio reikšmė kolektyvinio tapatumo formavimui; antra, reikia išanalizuoti SSRS kolektyvinio tapatumo formavimo trūkumus; trečia, reikia išanalizuoti ES kolektyvinio tapatumo formavimo ypatumus bei į perspektyvą nukreiptą formavimo politiką, įvertinant ekonominio veiksnio svarbą; ketvirtą, reikia palyginti ES ir Sovietų Sąjungos kolektyvinio tapatumo formavimo specifiką, remiantis gautais duomenimis, padaryti išvadas.
Darbe yra keliamos šios hipotezės:
1. ekonominis veiksnys yra svarbus kolektyvinio tapatumo formavimo procese;
2. kolektyvinio tapatumo formavimo specifika SSRS ir ES turi sąryšio taškų, tai yra - juos įtakoja tie patys veiksniai;
3. ES turi daugiau perspektyvų suformuoti kolektyvinį tapatumą nei SSRS.
Darbe taikomi tyrimo metodai: analitinis – aprašomasis... [toliau žr. visą tekstą]The title of this master thesis is “Formation of Collective Identity in USSR and EU: Impact of Economic Factor“. It defines historical period from USSR foundation till nowadays. The main aspect according to which these multinational blocks are analyzed is collective identity. Special efforts are given to analyze factors which have impact to collective identity formation, but the biggest attention is paid to analyze importance of economic factor. Identity – the sense of dependence. This concept at one time contains as the connective as also separative elements. This is a result of identification process which helps to define human or group exceptional indications. As individual or collective identity formation problems are widely discussed in today’s society discourses. Its importance is comprehensible as to the individual as also to the communities behavior motivation. The perception of identity allows to know better your historical or cultural roots, and also to know yourself and others better. But the concept of identity is so wide and variant, that it leaves a lot of place to the following discussions. This work appoints a lot of attention to collective identity forming and it’s specific. Actuality of the topic remains also in today’s political discourses undisputed – if we clear, how to construct common identity it would emerge a possibility to evaluate, what main mistakes in it forming maid USSR and what perspectives in common identity constructing has EU. This... [to full text]
12
Portugal-Nunes, Diogo João. "Bioethanol production from SSLs: S. stipitis vs S. cerevisiae." Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/11279.
Full textAbstract:
Mestrado em Biotecnologia - Biotecnologia Industrial e AmbientalO objectivo desta dissertação foi a comparação da produção de bioetanol de 2ª geração por Scheffersomyces stipitis (em suspensão e imobilizada) e por duas estirpes industriais de Saccharomyces cerevisiae. Os substratos utilizados para a realização das fermentações foram os Licores de Cozimento ao Sulfito Ácido (SSLs) provenientes de madeira de resinosas (HSSL) e de madeiras de folhosas (SSSL – Domsjö hydrolysate). O HSSL e o SSSL são sub-produtos da indústria de pastas de papel, resultando do processo de cozimento ao sulfito com magnésio e sódio, respetivamente. Além de lenhosulfonatos, estes SSLs contêm monossacarídeos, destacando-se a glucose, xilose e manose. O ácido acético, composto inibidor da fermentação alcoólica por leveduras, também está presente em concentrações relativamente elevadas (≥ 5.0 g . L-1). O HSSL foi pré-tratado físico-quimicamente e submetido a uma remoção biológica de inibidores com P. variotii, enquanto o Domsjö hydrolysate foi utilizado sem qualquer tratamento. S. stipitis e S. cerevisiae são leveduras extensamente estudadas devido à sua capacidade de fermentação de pentoses e hexoses, respetivamente. Num estágio prévio às fermentações dos SSLs, as leveduras foram pré-adaptadas em 60% HSSL ou 40% SSSL. Os máximos de rendimento (0.440 g etanol . g açúcares-1) e produtividade em etanol (0.885 g etanol . L-1 . h-1) foram obtidos nas fermentações com S. cerevisiae TMB 3500 em SSSL, sendo estas as variáveis com maior potencial para aplicação industrial. Embora a cultura suspensa de S. stipitis tenha resultado numa menor produtividade (0.010 g etanol . L-1 . h-1), a optimização do fornecimento de oxigénio ao biorreator deverá conduzir ao aumento da produtividade volumétrica em etanol. A imobilização celular e o controlo do pH em 5.5 nas fermentações com S. stipitis melhoraram a eficiência fermentativa ao aumentarem a produção de etanol em 1.3 e 1.6 vezes, respetivamente. Quando aplicadas simultaneamente, estas duas condições aumentaram o rendimento em etanol 2.2 vezes, sugerindo que (i) a imobilização numa matriz de alginato de cálcio protegeu a levedura dos inibidores químicos e que (ii) o controlo de pH em 5.5 foi determinante para a produção de etanol a partir de HSSL biologicamente pré-tratado. Os resultados comprovam que ambos os SSLs são potenciais substratos para a produção de bioetanol de 2ª geração usando S. stipitis ou S. cerevisiae, sob o conceito de biorefinaria.
The aim of this work was the comparison of second-generation bioethanol production by Scheffersomyces stipitis (free-culture and immobilized) and two Saccharomyces cerevisiae industrial strains, using Hardwood Spent Sulphite Liquor (HSSL) or Domsjö hydrolysate (SSSL) as substrate. HSSL and SSSL are side products of pulp and paper industry, from magnesium and sodium-based acidic sulphite pulping, respectively. Besides sulphonated lignin, SSLs contain fermentable sugars, mainly glucose, xylose and mannose. Acetic acid, a known inhibitor of ethanol fermentation by yeasts, is also present in a relatively high content (≥ 5.0 g . L-1). HSSL was previously physico-chemically pretreated and bio-detoxified with P. variotii, whereas SSSL was used without any treatment. S. stipitis and S. cerevisiae are the most widely studied pentose and hexose-fermenting yeasts, respectively. Before fermentations in SSLs, all yeast strains were pre-adapted by growing them in 60% HSSL or 40% SSSL. The highest maximum ethanol yield (0.440 g ethanol . g sugars-1) and productivity (0.885 g ethanol . L-1 . h-1) were obtained using S. cerevisiae TMB 3500 and SSSL. Suspended S. stipitis achieved a lower ethanol productivity (0.010 g ethanol . L-1 . h-1) but further optimization on the supplied oxygen in the fermentor might lead to higher ethanol productivity. Immobilization and pH control at 5.5 on S. stipitis fermentations improved the fermentation efficiency, increasing up the ethanol production by 1.3-fold and 1.6-fold, respectively. When applied simultaneously, these two conditions increased the ethanol yield 2.2-fold, suggesting that (i) immobilization with a calcium-alginate matrix protected the yeast from the inhibitory compounds and (ii) the controlled pH at 5.5 was essential for ethanol production from bio-detoxified HSSL. Results showed that both SSLs are potential substrates for the production of 2nd generation bioethanol by S. stipitis or S. cerevisiae, under the biorefinery concept.
13
PINABIAUX, NOWAK VERONIQUE, and PATRICE PINABIAUX. "Sclerose systemique sans sclerodermie : ssss ; a propos de 12 observations." Lille 2, 1991. http://www.theses.fr/1991LIL2M037.
Full text
14
Xia, Qianbin. "Towards Design and Analysis For High-Performance and Reliable SSDs." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/4904.
Full textAbstract:
NAND Flash-based Solid State Disks have many attractive technical merits, such as low power consumption, light weight, shock resistance, sustainability of hotter operation regimes, and extraordinarily high performance for random read access, which makes SSDs immensely popular and be widely employed in different types of environments including portable devices, personal computers, large data centers, and distributed data systems.
However, current SSDs still suffer from several critical inherent limitations, such as the inability of in-place-update, asymmetric read and write performance, slow garbage collection processes, limited endurance, and degraded write performance with the adoption of MLC and TLC techniques. To alleviate these limitations, we propose optimizations from both specific outside applications layer and SSDs' internal layer. Since SSDs are good compromise between the performance and price, so SSDs are widely deployed as second layer caches sitting between DRAMs and hard disks to boost the system performance. Due to the special properties of SSDs such as the internal garbage collection processes and limited lifetime, traditional cache devices like DRAM and SRAM based optimizations might not work consistently for SSD-based cache. Therefore, for the outside applications layer, our work focus on integrating the special properties of SSDs into the optimizations of SSD caches. Moreover, our work also involves the alleviation of the increased Flash write latency and ECC complexity due to the adoption of MLC and TLC technologies by analyzing the real work workloads.
15
Binti, Mohd Najib Norazwana. "Dual mode suspended substrate stripline (SSS) filter." Thesis, University of Leeds, 2018. http://etheses.whiterose.ac.uk/20593/.
Full textAbstract:
Evolution of wireless communication systems towards high flexibility, low cost and high efficiency leads to tremendous activity in the area of microwave filters. In an RF front-end of a cellular radio base station, signals are being transmitted and received simultaneously. In the receive band, there are chances of intermodulation products from the power amplifier being fed to the receiver,thus the transmit filter must have a very high level of signal rejection. Furthermore, the transmit filter must also have low passband insertion loss since it impacts the power transmitted and the overall transmit system efficiency. Recently, filters with dual-mode operation were being investigated due to their ability to produce two degenerate modes using a single physical structure; therefore, the size and cost of the filter can be reduced without compromising any figure-of-merits. A dual mode suspended substrate stripline filter is presented in this thesis. These filters enable achieving low insertion loss, high Q, high selectivity and good spurious response. Initially, a dual mode ring resonator structure is investigated using suspended substrate stripline technology. This technology is used due to its advantages which are comparable to microstrip or any other planar transmission lines. The HFSS three dimensional finite element method (FEM) is used to evaluate the resonant frequency, quality factor and the first harmonics. A second order suspended substrate stripline filter was designed with capacitive coupled input and output feeding method. The input and output feed were positioned 90 degree from each other while a notch was used in this filter to couple two degenerate modes which also control the bandwidth of the filter. A high performance Generalized Chebychev filter was designed to meet the stringent electrical requirement in the RF front-end of a cellular radio base station. With this fourth order filter, four finite frequency transmission zeros were achieved due to phase cancellation between two paths which results in high selectivity filter response. Metal tuning screws were added to improve any practical imperfections. Finally an asymmetrical Generalized Chebychev filter was designed with real frequency transmission zeros positioned on one side of the passband. With this design, the aim of achieving higher selectivity filter response above the passband was demonstrated.
16
Mauldin, Kendall. "SATELLITE GROUND STATION SECURITY USING SSH TUNNELING." International Foundation for Telemetering, 2003. http://hdl.handle.net/10150/605566.
Full textAbstract:
International Telemetering Conference Proceedings / October 20-23, 2003 / Riviera Hotel and Convention Center, Las Vegas, NevadaAs more satellite ground station systems use the Internet as a means of connectivity, the security of the ground stations and data transferred between stations becomes a growing concern. Possible solutions include software-level password authentication, link encryption, IP filtering, and several others. Many of these methods are being implemented in many different applications. SSH (Secure Shell) tunneling is one specific method that ensures a highly encrypted data link between computers on the Internet. It is used every day by individuals and organizations that want to ensure the security of the data they are transferring over the Internet. This paper describes the security requirements of a specific example of a ground station network, how SSH can be implemented into the existing system, software configuration, and operational testing of the revised ground network.
17
Flucke, Thomas J. "IDENTIFICATION OF USERS VIA SSH TIMING ATTACK." DigitalCommons@CalPoly, 2020. https://digitalcommons.calpoly.edu/theses/2208.
Full textAbstract:
Secure Shell, a tool to securely access and run programs on a remote machine, is an important tool for both system administrators and developers alike. The technology landscape is becoming increasingly distributed and reliant on tools such as Secure Shell to protect information as a user works on a system remotely. While Secure Shell accounts for the abuses the security of older tools such as telnet overlook, it still has fundamental vulnerabilities which leak information about both the user and their activities through timing attacks. The OpenSSH client, the implementation included in all Linux, Mac, and Windows computers, sends each keystroke entered to the server as soon as it becomes available. As a result, an attacker can observe the network patterns to know when a user presses a key and draw conclusions based on that information such as what a user is typing or who they are. In this thesis, we demonstrate that such an attack allows a malicious observer to identify a user with a concerning level of accuracy without having direct access to either the client or server systems. Using machine learning classifiers, we identify individual users in a crowd based solely on the size and timing of packets traveling across the network. We find that our classifiers were able to identify users with 20\% accuracy using as little as one hour of network traffic. Two of them promise to scale well to the number of users.
18
Wilkinson, Timothy James. "The Plastic Behaviour of Cold-Formed Rectangular Hollow Sections." University of Sydney. Department of Civil Engineering, 2000. http://hdl.handle.net/2123/843.
Full textAbstract:
The aim of this thesis is to assess the suitability of cold-formed rectangular hollow sections (RHS) for plastic design. The project involved an extensive range of tests on cold-formed Grade C350 and Grade C450 (DuraGal) RHS beams, joints and frames. A large number of finite element analyses was also carried out on models of RHS beams. The conclusion is that cold- formed RHS can be used in plastic design, but stricter element slenderness (b/t) limits and consideration of the connections, are required. Further research, particularly into the effect of axial compression on element slenderness limits, is required before changes to current design rules can be finalised. Bending tests were performed on cold-formed RHS to examine the web and flange slenderness required to maintain the plastic moment for a large enough rotation suitable for plastic design. The major conclusions of the beam tests were: (i) Some sections which are classified as Compact or Class 1 by current steel design specifications do not maintain plastic rotations considered sufficient for plastic design. (ii) The current design philosophy, in which flange and web slenderness limits are independent, is inappropriate. An interaction formula is required, and simple formulations are proposed for RHS. Connection tests were performed on various types of knee joints in RHS, suitable for the column - rafter connection in a portal frame. The connection types investigated were welded stiffened and unstiffened rigid knee connections, bolted plate knee joints, and welded and bolted internal sleeve knee joints, for use in RHS portal frames. The ability of the connections to act as plastic hinges in a portal frame was investigated. The most important finding of the joint tests was the unexpected fracture of the cold-formed welded connections under opening moment before significant plastic rotations occurred. The use of an internal sleeve moved the plastic hinge in the connection away from the connection centre- line thus eliminating the need for the weld between the RHS, or the RHS and the stiffening plate, to carry the majority of the load. The internal sleeve connections were capable of sustaining the plastic moment for large rotations considered suitable for plastic design. Tests on pinned-base portal frames were also performed. There were three separate tests, with two different ratios of vertical to horizontal point loads, simulating gravity and horizontal wind loads. Two grades of steel were used for comparison. The aims of the tests were to examine if a plastic collapse mechanism could form in a cold-formed RHS frame, and to investigate if plastic design was suitable for such frames. In each frame, two regions of highly concentrated curvature were observed before the onset of local buckling, which indicated the formation of plastic hinges and a plastic collapse mechanism. An advanced plastic zone structural analysis which accounted for second order effects, material non-linearity and member imperfections slightly overestimated the strength of the frames. The analysis slightly underestimated the deflections, and hence the magnitude of the second order effects. A second order plastic zone analysis, which did not account for the effects of structural imperfections, provided the best estimates of the strengths of the frames, but also underestimated the deflections. While cold-formed RHS did not satisfy the material ductility requirements specified for plastic design in some current steel design standards, plastic hinges and plastic collapse mechanisms formed. This suggests that the restriction on plastic design for cold-formed RHS based on insufficient material ductility is unnecessary, provided that the connections are suitable for plastic hinge formation, if required. A large number of finite element analyses were performed to simulate the bending tests summarised above, and to examine various parameters not studied in the experimental investigation. To simulate the experimental rotation capacity of the RHS beams, a sinusoidally varying longitudinal local imperfection was prescribed. The finite element analysis determined similar trends as observed experimentally, namely that the rotation capacity depended on both the web slenderness and flange slenderness, and that for a given section aspect ratio, the relationship between web slenderness and rotation capacity was non-linear. The main finding of the finite element study was that the size of the imperfections had an unexpectedly large influence on the rotation capacity. Larger imperfections were required in the more slender sections to simulate the experimental results. There should be further investigation into the effect of varying material properties on rotation capacity.
19
Wilkinson, Timothy James. "The Plastic Behaviour of Cold-Formed Rectangular Hollow Sections." Thesis, The University of Sydney, 1999. http://hdl.handle.net/2123/843.
Full textAbstract:
The aim of this thesis is to assess the suitability of cold-formed rectangular hollow sections (RHS) for plastic design. The project involved an extensive range of tests on cold-formed Grade C350 and Grade C450 (DuraGal) RHS beams, joints and frames. A large number of finite element analyses was also carried out on models of RHS beams. The conclusion is that cold- formed RHS can be used in plastic design, but stricter element slenderness (b/t) limits and consideration of the connections, are required. Further research, particularly into the effect of axial compression on element slenderness limits, is required before changes to current design rules can be finalised. Bending tests were performed on cold-formed RHS to examine the web and flange slenderness required to maintain the plastic moment for a large enough rotation suitable for plastic design. The major conclusions of the beam tests were: (i) Some sections which are classified as Compact or Class 1 by current steel design specifications do not maintain plastic rotations considered sufficient for plastic design. (ii) The current design philosophy, in which flange and web slenderness limits are independent, is inappropriate. An interaction formula is required, and simple formulations are proposed for RHS. Connection tests were performed on various types of knee joints in RHS, suitable for the column - rafter connection in a portal frame. The connection types investigated were welded stiffened and unstiffened rigid knee connections, bolted plate knee joints, and welded and bolted internal sleeve knee joints, for use in RHS portal frames. The ability of the connections to act as plastic hinges in a portal frame was investigated. The most important finding of the joint tests was the unexpected fracture of the cold-formed welded connections under opening moment before significant plastic rotations occurred. The use of an internal sleeve moved the plastic hinge in the connection away from the connection centre- line thus eliminating the need for the weld between the RHS, or the RHS and the stiffening plate, to carry the majority of the load. The internal sleeve connections were capable of sustaining the plastic moment for large rotations considered suitable for plastic design. Tests on pinned-base portal frames were also performed. There were three separate tests, with two different ratios of vertical to horizontal point loads, simulating gravity and horizontal wind loads. Two grades of steel were used for comparison. The aims of the tests were to examine if a plastic collapse mechanism could form in a cold-formed RHS frame, and to investigate if plastic design was suitable for such frames. In each frame, two regions of highly concentrated curvature were observed before the onset of local buckling, which indicated the formation of plastic hinges and a plastic collapse mechanism. An advanced plastic zone structural analysis which accounted for second order effects, material non-linearity and member imperfections slightly overestimated the strength of the frames. The analysis slightly underestimated the deflections, and hence the magnitude of the second order effects. A second order plastic zone analysis, which did not account for the effects of structural imperfections, provided the best estimates of the strengths of the frames, but also underestimated the deflections. While cold-formed RHS did not satisfy the material ductility requirements specified for plastic design in some current steel design standards, plastic hinges and plastic collapse mechanisms formed. This suggests that the restriction on plastic design for cold-formed RHS based on insufficient material ductility is unnecessary, provided that the connections are suitable for plastic hinge formation, if required. A large number of finite element analyses were performed to simulate the bending tests summarised above, and to examine various parameters not studied in the experimental investigation. To simulate the experimental rotation capacity of the RHS beams, a sinusoidally varying longitudinal local imperfection was prescribed. The finite element analysis determined similar trends as observed experimentally, namely that the rotation capacity depended on both the web slenderness and flange slenderness, and that for a given section aspect ratio, the relationship between web slenderness and rotation capacity was non-linear. The main finding of the finite element study was that the size of the imperfections had an unexpectedly large influence on the rotation capacity. Larger imperfections were required in the more slender sections to simulate the experimental results. There should be further investigation into the effect of varying material properties on rotation capacity.
20
Pieri, Naira Caroline Godoy. "Espermatogênese xenogênica pós-transplante de células tronco caninas no testículo de camundongos." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-10052016-120531/.
Full textAbstract:
As células tronco espermatogoniais (SSCs) são caracterizadas pela capacidade de autorrenovação, proliferação e transmissão das informações genéticas. Em caninos a primeira tentativa de xenotransplante não obteve o sucesso da produção de espermatozoides, no entanto, há evidências de que as células testiculares xenogênicas podem ser transplantadas no testículo do animal hospedeiro, e gerar espermatozoides viáveis do doador. Portanto, este estudo tem como objetivo realizar o xenotransplante das células germinativas caninas em camundongos imunosuprimidos, e com isto promover à produção de espermatozoides caninos viáveis, geneticamente modificados. E por meio desta técnica, analisar a eficiência da espermatogênese pós-transplante. Células germinativas testiculares foram caracterizadas, isoladas e cultivadas de cães pré-púberes, por meio de sistemas de cultura de enriquecimento e fatores de crescimento. As células foram transduzidas com um gene repórter GFP e LacZ, e por um vetor lentiviral para indentificar as SSCs nos testículos receptores. As SSCs transduzidas foram transplantadas nos testículos de camundongos (C57BL/6) tratados com Busulfan, após diferentes períodos os animais receptores foram eutanasiados e analisados. Aos 10 dias de cultivo as células germinativas adultas foram positivas para CD49f, CD117, e com 5 dias uma expressão semelhante de GFRA1 e DAZL, demonstrando a presença de SSCs e algumas células em meiose. Transplantamos 105 células e 20-43% das células transplantadas foram identificadas na membrana basal dos túbulos seminíferos do animal receptor. Portanto, o transplante das células germinativas caninas, mostrou que a purificação e o cultivo realizados são possíveis para obter SSCs caninas, as quais colonizaram os túbulos seminíferos dos camundongos imunodeficientes e mantiveram-se vivas na membrana basal por 90 dias após transplante, mesmo que estes animais tenham distância filogenéticaThe spermatogonial stem cells (SSCs) are characterized by the capacity for self-renewal, proliferation and transmission of genetic information. In canines the first attempt to xenotransplantation did not achieve the success of sperm production. However, there is evidence that testicular xenogeneic cells can be transplanted into the testis of the host animal, and generate viable sperm donor. Therefore, this study aims conduct xenotransplantation of the canine germ cells in immunosuppressed mice, and thereby promote the production of viable sperm canines, genetically modified. And by this technique, analyze the efficiency of post-transplant spermatogenesis. Testicular germ cells were identified, isolated and cultured prepuberes dogs through enrichment culture systems and growth factors. Cells were modificated with a reporter gene GFP and LacZ. The SSCs canine was transplanted in mice (C57Bl/6), after different period and then the recipient animals were euthanized and analyzed. After 10 days in culture the germ cells were positive for CD49f, CD117, and 5 days a similar expression of GFRA1 and DAZL was observed, demonstrating the presence of SSCs and some cells in meiosis. 105 cells were transplanted and 20-43% of the transplanted cells were identified in the basement membrane of the seminiferous tubules after 90 days after transplantation. Therefore, the transplantation of canine germ cells showed that the cultivation and purification are performed possible for canine SSCs, it can colonize the seminiferous tubules the mice infertility remained alive in the basement membrane for 90 days after transplanting, even though these animals have phylogenetic distance
21
Oyebanji, Akanbi Olusayo. "Development of a framework for Sustainable Social Housing Provision (SSHP) in England." Thesis, University of Central Lancashire, 2014. http://clok.uclan.ac.uk/11321/.
Full textAbstract:
Sustainable social housing provision (SSHP) has become very important over the last few decades for the main objective of meeting housing needs. Despite this, there is evidence to suggest that SSHP has not been practiced successfully. A growing body of the literature shows that lack of an acceptable definition of social housing could contribute to the lack of political will for achieving SSHP. The overall aim of this research is to develop ‘a framework for implementing SSHP’. The aim was achieved through research objectives, including a critical review of the concept and identification of types of social housing and proposes a definition for describing it; examine the concept of sustainable development (SD) and its requirements for achieving sustainability in social housing provision (SHP); and examine the key constituents, barriers and recommendations for improving SSHP from economic, environmental and social perspectives. The methodology adopted for this research comprises a combination of the review of the extant literature, a qualitative content analysis and a quantitative questionnaire survey. The contents of the selected documents were grouped into three main categories – constituents, barriers and recommendations for implementing SSHP with economic, environmental and social key factors as sub-groups for each. Data gathered through the questionnaire survey were obtained from housing authorities (public sector) and housing associations (non-profit private sector) as social housing practitioners in England and were analysed using various statistical analysis, including ANOVA. Findings from the study assisted in ranking the key constituents, barriers and recommendations for the implementation of SSHP from economic, environmental and social perspectives, which are categorised into most critical, critical and less critical for achieving sustainability in SHP. The main factors that dominate SSHP include: affordability, adequate provision, adequate funding, economic design and planning, use of environmental friendly materials, effective land use, use of the renewable energy, reduction of waste, promotion of social cohesion, security of lives and property, etc. The outcome was used to develop a framework for improving the implementation of SSHP, which has been tested and validated. Although SD has become a dominant focus of research activities in recent years, studies undertaken for the development of a framework that tied constituents with barriers and recommendations for implementing SSHP are rare. The framework of this type can help to address various sustainability issues that militate against the achievement of sustainability in SHP. In order to achieve SSHP, the role of stakeholders, including social housing practitioners, governments/agencies, financial institutions and end-users are significant. The study concluded that there is a need to develop a framework for implementing SSHP with a strong recommendation that stakeholders should effectively address sustainability issues in SHP.
22
Lokaj, Lukáš. "Analýza správy podnikových dokumentů v SSČS a.s. a návrh na její optimalizaci." Master's thesis, Vysoká škola ekonomická v Praze, 2013. http://www.nusl.cz/ntk/nusl-199591.
Full textAbstract:
This thesis focuses on design of document exchange solution used between Stavební spořitelna České spořitelny a.s. (SSČS) and its external sales network, which is outdated and doesn't meet the needs of SSČS or sales network. The thesis also includes the identification of suitable basic principles used in the service-oriented architecture (SOA) concept and their application in design solutions. The aim of the thesis is a conceptual solution design, based on the principles of SOA and the following technological design of changes in existing environment, so that on this basis could be created a functional and technical specification for suppliers of these applications. Stated objective is achieved through an initial analysis of the current environment, which identifies the possibilities of existing applications, and identifies weaknesses in the process that will be changed in the new solution. The analysis covers as the environment of external sales network as the related environment in SSČS, which together figure in the process of documentation exchange. As the results of the analysis and based on theoretical knowledge and consultation with experts in the field of SOA is then formulated a proposed solution at the conceptual level, which is further elaborated in the description of the proposed changes in individual components of the new solution. The outputs of this thesis will serve SSČS both in terms of describing the existing and the new environment, and as an input for the specifications of the proposed amendments to the supplier concerned applications. Structure of the thesis consists of introduction, devoted to the characterization of SSČS and providing an initial framework for solved domain. This is followed by a description and the analysis of external sales network, particularly in terms of application equipment and communication flows, followed by a description of the environment in SSČS, which contains the components processing sent documents. Next part of thesis is devoted to the formulation of the basic concepts of SOA and selection of appropriate principles that are followed in the subsequent section to draft the solution itself, first at the conceptual level, followed by a detailed description of the necessary changes. Everything is then evaluated in the final part.
23
Zhang, Jin. "Immunological modulation of antioxidants in side-stream cigarette smoke (SSCS) exposed mice." Diss., The University of Arizona, 2002. http://hdl.handle.net/10150/280048.
Full textAbstract:
Environmental tobacco smoke (ETS) is a complex mixture of chemicals generated during the burning of tobacco products. The principle contributor to ETS is side-stream cigarette smoke (SSCS), the material emitted from the smoldering tobacco product between puffs Our hypothesis is that reactive oxygen species from SSCS are playing an essential role in disease promotion and antioxidant supplementation (a single form of alpha-tocopherol or a mixture of multiple antioxidants) will potentially prevent SSCS associated tissue damage, pulmonary dysfunction. The specific aims of the present study are to determine if: (1) SSCS would induce tissue lipid peroxidation and proinflammatory responses; (2) SSCS would provoke pulmonary and cardiac function changes; (3) SSCS would cause oxidative stress, reduce nutrient concentrations and suppress immune function in murine retrovirus infections; (4) dietary alpha-tocopherol, specifically, can enhance resistance to oxidative damage by SSCS and improve lung function; (5) multiple antioxidant supplementation can modulate proinflammatory cytokine secretion and tissue lipid peroxidation induced by SSCS exposure in old healthy mice. SSCS exposure methodology in a murine model was developed to facilitate these goals. For the first time we established a SSCS model in murine retrovirus infection. Also we conduced a SSCS dose-response model for a cardiac function study. We found that SSCS exposure in mice consistently increased oxidation, depleted tissue vitamin E levels, and promoted inflammatory cytokines production. SSCS exposure at 120-min/day, 5 days/week for 12 weeks decreased heart contractile function and increased vascular resistance. SSCS induced increased oxidative stress, reduced nutrient concentrations and suppressed immune function, which could make mice with murine retrovirus more susceptible to opportunistic infections. Dietary alpha-tocopherol enhanced resistance against SSCS-induced oxidation and improved lung function, primarily through the antioxidant property of alpha-tocopherol and its modulation of local cytokine production. The multiple antioxidant with beta-carotene, bioflavanoids, Coenzyme Q10, d-alpha-tocopherol, L-ascorbic acid, L-carnitine, magnesium, N-acetylcysteine, retinol, selenium and zinc given as a dietary supplementation prevented oxidation and IL-6 production in healthy old mice during SSCS exposure. (Abstract shortened by UMI.)
24
Cholasta, Jan. "Správa veřejných klíčů SSH v programech FreeIPA a SSSD." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2012. http://www.nusl.cz/ntk/nusl-236572.
Full textAbstract:
SSH je jeden z nejpoužívanějších protokolů pro vzdálený přístup v Internetu. SSH je flexibilní a rozšiřitelný protokol, který se skládá ze tří hlavních součástí: SSH transportního protokolu, který obstarává důvěrnost, integritu a autentizaci serveru, SSH autentizačního protokolu, který obstarává autentizaci uživatelů a SSH spojovacího protokolu, který obstarává multiplexování více kanálů různých typů (interaktivní sezení, přesměrování TCP/IP spojení, atd.) do jednoho spojení. OpenSSH je jedna z nejrozšířenějších implemetací SSH. OpenSSH obsahuje SSH server, SSH klienty, generátor SSH klíčů a autentizační agent, který usnadňuje autentizaci pomocí veřejných klíčů. FreeIPA a SSSD jsou projekty poskytující centrální správu identit pro Linuxové a Unixové systémy. Tyto projekty sice v době psaní této práce přímou podporu SSH neobsahovaly, ale do jisté míry je ve spojení s OpenSSH používat možné bylo.
25
今村, 敦司, A. IMAMURA, 高弘 藤田, T. FUJITA, 正泰 嘉賀, M. KAGA, 成仁 水谷, et al. "4．SSH評価部会中間報告 A: SSH評価グループ (VI.研究グループ中間報告)." 名古屋大学教育学部附属中学校 : 名古屋大学教育学部附属高等学校, 2006. http://hdl.handle.net/2237/9541.
Full text
26
Malhotra, Vasudha. "Teachers' perspectives on teaching of socio-scientific issues (SSIs) in Indian science classrooms." Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/15428/.
Full textAbstract:
This thesis explores the teachers’ perspectives on the teaching of Socio-Scientific Issues (SSIs) and the factors that influence their teaching. SSIs are those contemporary issues that are deep rooted in sciences, have an impact on society, involve moral and ethical reasoning and are controversial in nature. The study has been conducted in the context of Indian science classrooms, with fourteen upper primary and secondary stage science teachers from five schools. Three of the five selected schools are Green Schools, which have an explicit focus on the teaching of environmental SSIs. The data was collected using semi-structured interviews and classroom observation sessions, with a focus on following two questions. (1) What are the methods and strategies used by the teachers for teaching SSIs? (2) What are the factors that influence the teaching of SSIs? The results showed that the majority of teachers supported the teaching of SSIs and for some this links to specific SSIs in their lives and local communities in their Indian context, particularly pollution and environmental threats. However, a significant level of variation was witnessed in their actual classroom practices for teaching SSIs. The various profiles of teachers, classified based on their beliefs and classroom practices, have been discussed. Among the methods used by the teachers for teaching SSIs, the two most common method for teaching SSIs were discussions and lecture method. Four different forms of discussions and two different forms of lecture method have been delineated based on the strategies used by the teachers for teaching SSIs. Results indicated that teachers’ beliefs, school leadership and assessment system are the most influential factors for SSIs teaching. Implications and the major findings of this research have been discussed which contribute towards the acute lack of empirical studies over the teaching of SSIs in Indian schools.
27
Menon, Smita Kesavankutty. "X-ray crystallographic studies of the proteins from sulfolobus spindle-shaped viruses (SSVs)." Thesis, Montana State University, 2009. http://etd.lib.montana.edu/etd/2009/menon/MenonS0809.pdf.
Full textAbstract:
Viruses populate virtually every ecosystem on the planet. Fuselloviridae are ubiquitous crenarchaeal viruses found in high-temperature acidic hot springs around the world. However, compared to eukaryotic and bacterial viruses, our knowledge of viruses infecting the archaea is limited. Fuselloviral genomes show little similarity to other organisms, generally precluding functional predictions. However, structural studies can reveal distant evolutionary relationships and provide functional insights that are not apparent from the primary amino acid sequence alone. Several such structural studies have already contributed to our understanding of the Sulfolobus Spindle-shaped viruses (Fuselloviridae). Here we report the structure of two proteins, SSV1 F112 and SSVRH D212. Biochemical, proteomic and structural studies of F112 reveal a monomeric intracellular protein that adopts a winged helix DNA binding fold. Continuing these efforts, a second structure was also determined where the overall fold and conservation of active site residues place D212 within the PD-(D/E)XK nuclease superfamily. Notably, the structure of F112 contains an intrachain disulfide bond, prompting analysis of cysteine usage in this and other hyperthermophilic viral genomes. The analysis supports a general abundance of disulfide bonds in the intracellular proteins of hyperthermophilic viruses and the evolutionary implications of such distribution are discussed. Here we review and describe our progress towards understanding these viruses at a molecular level.
28
Britton, Warner H. Eaves Ronald C. "The Sensation Seeker Attention Scale (SSAS) a measure of sensation seeking by adolescents /." Auburn, Ala., 2007. http://repo.lib.auburn.edu/2007%20Fall%20Dissertations/Britton_Warner_10.pdf.
Full text
29
Polato, Ivanilton. "Energy savings and performance improvements with SSDs in the Hadoop Distributed File System." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/45/45134/tde-31102016-155908/.
Full textAbstract:
Energy issues gathered strong attention over the past decade, reaching IT data processing infrastructures. Now, they need to cope with such responsibility, adjusting existing platforms to reach acceptable performance while promoting energy consumption reduction. As the de facto platform for Big Data, Apache Hadoop has evolved significantly over the last years, with more than 60 releases bringing new features. By implementing the MapReduce programming paradigm and leveraging HDFS, its distributed file system, Hadoop has become a reliable and fault tolerant middleware for parallel and distributed computing over large datasets. Nevertheless, Hadoop may struggle under certain workloads, resulting in poor performance and high energy consumption. Users increasingly demand that high performance computing solutions address sustainability and limit energy consumption. In this thesis, we introduce HDFSH, a hybrid storage mechanism for HDFS, which uses a combination of Hard Disks and Solid-State Disks to achieve higher performance while saving power in Hadoop computations. HDFSH brings, to the middleware, the best from HDs (affordable cost per GB and high storage capacity) and SSDs (high throughput and low energy consumption) in a configurable fashion, using dedicated storage zones for each storage device type. We implemented our mechanism as a block placement policy for HDFS, and assessed it over six recent releases of Hadoop with different architectural properties. Results indicate that our approach increases overall job performance while decreasing the energy consumption under most hybrid configurations evaluated. Our results also showed that, in many cases, storing only part of the data in SSDs results in significant energy savings and execution speedupsAo longo da última década, questões energéticas atraíram forte atenção da sociedade, chegando às infraestruturas de TI para processamento de dados. Agora, essas infraestruturas devem se ajustar a essa responsabilidade, adequando plataformas existentes para alcançar desempenho aceitável enquanto promovem a redução no consumo de energia. Considerado um padrão para o processamento de Big Data, o Apache Hadoop tem evoluído significativamente ao longo dos últimos anos, com mais de 60 versões lançadas. Implementando o paradigma de programação MapReduce juntamente com o HDFS, seu sistema de arquivos distribuídos, o Hadoop tornou-se um middleware tolerante a falhas e confiável para a computação paralela e distribuída para grandes conjuntos de dados. No entanto, o Hadoop pode perder desempenho com determinadas cargas de trabalho, resultando em elevado consumo de energia. Cada vez mais, usuários exigem que a sustentabilidade e o consumo de energia controlado sejam parte intrínseca de soluções de computação de alto desempenho. Nesta tese, apresentamos o HDFSH, um sistema de armazenamento híbrido para o HDFS, que usa uma combinação de discos rígidos e discos de estado sólido para alcançar maior desempenho, promovendo economia de energia em aplicações usando Hadoop. O HDFSH traz ao middleware o melhor dos HDs (custo acessível por GB e grande capacidade de armazenamento) e SSDs (alto desempenho e baixo consumo de energia) de forma configurável, usando zonas de armazenamento dedicadas para cada dispositivo de armazenamento. Implementamos nosso mecanismo como uma política de alocação de blocos para o HDFS e o avaliamos em seis versões recentes do Hadoop com diferentes arquiteturas de software. Os resultados indicam que nossa abordagem aumenta o desempenho geral das aplicações, enquanto diminui o consumo de energia na maioria das configurações híbridas avaliadas. Os resultados também mostram que, em muitos casos, armazenar apenas uma parte dos dados em SSDs resulta em economia significativa de energia e aumento na velocidade de execução
30
Tarnowski, Christian Gabriel. "Desenvolvimento e caracterização de marcadores microssatélites (SSRs) para Cedrela Lilloi C. de Candolle." reponame:Repositório Institucional da UFSC, 2012. http://repositorio.ufsc.br/xmlui/handle/123456789/94114.
Full textAbstract:
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Florianópolis, 2010Made available in DSpace on 2012-10-25T06:44:29Z (GMT). No. of bitstreams: 1 287627.pdf: 1226059 bytes, checksum: 2337976f95da6827275c91baaf2701fc (MD5)
Cedrela lilloi (Meliaceae) é uma das quatro espécies de cedro nativas da Argentina e encontra-se estritamente distribuída na Selva de Yungas na região noroeste do país. Como tantas outras espécies florestais de importância econômica, as populações de C. lilloi encontram-se vulneráveis a exploração e fragmentação. No país são escassos os estudos referentes à diversidade e estrutura genética destas populações. Assim, os objetivos principais deste estudo foram a) desenvolver marcadores microssatélites específicos para a espécie e, b) estimar a variabilidade genética de três populações naturais. O DNA genômico de C. lilloi foi digerido com enzima, purificado, enriquecido através de sondas de oligos CT(8), GT(8) e TTC(8), clonado em bactérias e finalmente isolado e sequenciado. Dos 258 clones sequenciados, a porcentagem total de sequências microssatélites observadas foi muito baixa (14%). Foram desenhados iniciadores para essas 37 regiões microssatélites encontradas e somente quatro locos amplificaram produtos polimórficos. Devido a que os poucos locos desenvolvidos não foram suficientes para analisar as populações, foi considerado a alternativa da transferibilidade. Foram transferidos cinco iniciadores provenientes de outras espécies da família Meliaceae. No total, foram utilizados oito locos, três próprios de C. lilloi e cinco transferidos (média de 5,25 alelos), para analisar três populações com um total de 140 indivíduos. Nenhum loco apresentou desequilíbrio de ligação e não foi observada a presença de alelos nulos. A heterozigosidade observada (Ho) total foi de 0,406 e a esperada (He) de 0,416. O índice de fixação dentro das populações (FIS=0,035) não foi estatisticamente diferente de zero, indicando que a probabilidade de acasalamento entre indivíduos aparentados não é diferente do esperado no equilíbrio de Hardy-Weinberg. A endogamia a nível grupal (FIT=0,140) e a diferenciação genética entre as populações foram iguais e moderadas (FST=0,108), indicando que as populações estão estruturadas por causa da perda de alelos devido à deriva genética. As duas populações da região norte das Yungas (Baritú e San Andrés) foram geneticamente mais similares entre si e apresentaram maior diversidade gênica em comparação a população amostrada na região sul (El Siambón).
31
Schmidt, Andréa Branco. "Desenvolvimento e caracterização de marcadores microssatélites (SSRs) para Araucaria angustifolia (Bert.) O. Kuntze." Florianópolis, SC, 2005. http://repositorio.ufsc.br/handle/123456789/103108.
Full textAbstract:
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-Graduação em Recursos Genéticos Vegetais.Made available in DSpace on 2013-07-16T02:57:05Z (GMT). No. of bitstreams: 0
Este trabalho apresenta os resultados do desenvolvimento e caracterização de marcadores microssatélites para Araucaria angustifolia a partir de bibliotecas genômicas enriquecidas para seqüências microssatélites (SSR). O trabalho envolveu clonagem de segmentos genômicos, transformação bacteriana, sequenciamento de clones, desenho de iniciadores específicos e otimização de marcadores. A triagem dos iniciadores foi feita utilizando-se inicialmente quatro indivíduos em gel de agarose 2%. Para a otimização foram utilizados 16 indivíduos de 5 diferentes populações em gel de poliacrilamida 4%, com detecção em nitrato de prata. Foram obtidos 29 marcadores microssatélites inéditos para Araucaria angustifolia, com a possibilidade de utilização imediata. Os marcadores desenvolvidos revelaram elevada heterozigosidade esperada para os locos, com uma detecção média de 8,1 alelos por loco. Os marcadores microssatélites demonstraram elevado conteúdo informativo para os estudos de diversidade genética e discriminação genotípica de indivíduos. As sequências originais contendo regiões microssatélites foram depositadas no GenBank (NCBI - National Center for Biotechnology Information). Um estudo de diversidade genética e estrutura de duas populações naturais de Araucaria angustifolia foi desenvolvido utilizando-se locos microssatélites específicos desenvolvidos para a espécie. Para realizar a genotipagem das duas populações: Guamirim Gateado e Parque Ecológico de Lages, foram utilizados dois sistemas de detecção alélica colorimétrico baseado em nitrato de prata, aplicado a sete locos microssatélites e outro com detecção por fluorescência aplicado a nove locos microssatélites. Os resultados mostram que, grande parte da variabilidade genética observada nas duas populações encontra-se dentro das populações e não entre as populações. As análises feitas a partir de genotipagens em sistema com nitrato de prata, superestimaram os valores de heterozigosidade. O sistema de detecção fluorescente mostrou-se necessário quando os marcadores microssatélites são baseados em seqüências de di-nucleotídeo, pois facilitam a análise em sistema "multiplex" utilizados na mesma reação de amplificação e/ou analisados conjuntamente na mesma eletroforese, propiciando redução de gastos e maior confiabilidade nos resultados devido à leitura automática do sistema, baseado no marcador de tamanho de fragmento conhecido. As comparações das análises populacionais feitas nos distintos sistemas de detecção revelaram que o sistema de detecção com fluorescência, é significativamente mais preciso devido à presença de bandas fantasmas, formadas pela separação das duas fitas de DNA durante a eletroforese, detectadas nas análises com nitrato de prata. Diferenças estatisticamente significativas entre os valores de heterozigosidade das duas populações estudadas foram obtidas com marcadores isoenzimáticos, fato este que não foi confirmado com o uso de marcadores microssatélites (0,87 e 0,86 para He e de 0,59 e 0,58 para Ho, respectivamente). Marcadores isoenzimáticos revelaram diferença estatística na estrutura genética entre as duas populações. No estudo com marcadores microssatélites não foi detectada divergência significativa entre as duas populações estudadas (FST=0,023). (Instituição financiadora: FAO - Food and Agriculture Organization of the United Nations/Ministério do Meio Ambiente).
32
Zielinski, Mateusz. "Système distribué actif sans fil basse consommation pour l'amortissement des vibrations." Thesis, Ecully, Ecole centrale de Lyon, 2015. http://www.theses.fr/2015ECDL0029/document.
Full textAbstract:
Depuis des siècles nous utilisons des véhicules équipés des systèmes de suspension de vibrations. Ils permettent d'avoir un confort acceptable et ajoutent de la sécurité à la conduite. Les nouveaux systèmes installés dans les véhicules sont des systèmes actifs. Ils peuvent être adaptés selon les exigences en temps réel. Ces types de systèmes sont utilisés pour l'amortissement de vibrations et pour l’isolation vibro-acoustique. Dans la thèse nous présentons une nouvelle approche d'un système adaptatif pour les applications automobiles. Nous faisons l'hypothèse qu’un portage d'un système centralisé en système distribué peut améliorer son efficacité. Nous proposons un réseau de capteurs sans fil pour l’amortissement de vibrations dans les applications automobiles. Un capteur du réseau est capable de mesurer des vibrations, d’amortir des vibrations et de récupérer l’énergie depuis les vibrations en utilisant un seul élément piézoélectrique (la méthode Serial-SSHI). Ensuite nous validons le réseau de capteurs sur une structure mécanique de type plaque. Les mesures sont comparées avec des simulations d’éléments finis. Les résultats des mesures et des simulations confirment le choix des solutions. Le nœud du réseau fournit ses fonctionnalités destinées avec une efficacité acceptable. Nous validons la récupération d’énergie depuis les vibrations et la mesure des vibrations. Ensuite nous validons un effet local d’amortissement de vibrations et un effet global (le réseau de capteurs permet d’avoir une action d’amortissement complémentaire)For centuries we have used vehicles equipped with the vibration suspension systems. These systems are used to provide comfort and safety. Nowadays we are implementing the active systems which can be adapted according to the real-time requirements. These types of systems are used to damp vibrations and to provide noise and vibration insulation. In the thesis we present a new approach of an adaptive system for automotive applications. We assume that a porting of a centralized system in a distributed system can improve its effectiveness. We offer a wireless sensor network for damping vibration in automotive applications. A network sensor is able to measure the vibrations, damp the vibrations and energy harvesting from vibrations by using a single piezoelectric element (Serial-SSHI method). We validate the network of nodes on a mechanical structure. The measurements are compared with finite element simulations. The results of measurements and simulations confirm the choice of solutions. The network node provides designed functionality with acceptable efficiency. We also validate the energy harvesting and the vibration measurements. The outcome of the work confirm a local effect of vibrations damping and a global effect (the designed Wireless Sensor Network provides a supplementary damping action)
33
Jeschke, Jonathan M. "Funktionelle Reaktionen von Konsumenten die SSS-Gleichung und ihre Anwendung /." [S.l.] : [s.n.], 2002. http://edoc.ub.uni-muenchen.de/archive/00000515.
Full text
34
Jeschke, Jonathan Michael. "Funktionelle Reaktionen von Konsumenten: die SSS Gleichung und ihre Anwendung." Diss., lmu, 2002. http://nbn-resolving.de/urn:nbn:de:bvb:19-5159.
Full text
35
Bashir, Muhammad Shahid, and Mohd Zalmy Zakaria. "Hesitation on adoption Self-Service Technologies (SSTs) : A case study on self payment machine." Thesis, Umeå University, Department of Informatics, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-34814.
Full textAbstract:
Self service providers are making such systems which offer faster and more flexible service to the user. Most of us are familiar about self service technologies such as Kiosk, Auto Teller Machine, Self Check in machine, Self payment machine etc. Unfortunately, users are not using these SSTs commonly as advancement has been occurring in SSTs. Sometimes, people look annoyed and feel fear to use such kind of technology at public spaces. Generally, these systems need interaction between users and technology to create service outcome instead of interacting with a service personnel. These technological interfaces have been called Self-service technologies (SSTs). Yet, not all users choose to use the new technologies and they still feel some hesitations to adopt this technologies in this case SSTs. This study investigated the factors that make users hesitate to use and adopt SSTs.
36
Kauschke, Christina. "Frühe lexikalische Verzögerung als Indikator für SSES? : Neue Befunde zur Entwicklung von Late Talkern." Universität Potsdam, 2008. http://opus.kobv.de/ubp/volltexte/2008/1890/.
Full textAbstract:
Auszug:
Seit langem ist bekannt, dass Kinder mit Sprachentwicklungsstörungen meist schon in einem frühen Stadium ihrer Sprachentwicklung auffallen: die ersten Wörter werden verspätet produziert, der Wortschatz wächst langsamer an, der Vokabularspurt setzt verzögert ein oder bleibt aus, so dass das produktive Vokabular mit zwei Jahren weniger als 50 Wörter umfasst. Außerdem treten keine Wortkombinationen auf. Obwohl bei nahezu jeder Sprachentwicklungs-störung retrospektiv derartige frühe Anzeichen auszumachen sind, mündet andererseits nicht jede frühe Verzögerung in eine anhaltende Störung. Diese Beobachtung hat Anlass zu einer regen Forschungstätigkeit gegeben. Im Zentrum steht die Frage, anhand welcher Kriterien sich der weitere Entwicklungsverlauf eines sprachlich verzögerten Kindes prognostizieren lässt. Der Forschungsstand zu diesem Bereich wurde an anderer Stelle bereits ausführlich beschrieben (z. B. in Kauschke 2000, 2003, 2006a). In diesem Beitrag möchte ich die Entwicklungswege dieser Population anhand aktueller Studien skizzieren, die Problematik der Prädiktion ansprechen und eine Studie über Sprachentwicklungsverläufe im dritten Lebensjahr vorstellen, in der ungestörte Kinder und sprachlich verzögerte längsschnittlich beobachtet wurden. Es folgen Überlegungen zur Therapienotwendigkeit und Therapiekonzeption, die durch die Darstellung eines exemplarischen Therapieverlaufs bei einem Einzelfall illustriert werden. [...]
37
Lokusethu, Hewage Don Danuska Hasitha. "TBM tunnelling through unfavourable ground conditions : a case study, SSDS tunnel F, Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/192957.
Full textAbstract:
A study of the Strategic Sewage Disposal Scheme (SSDS) Stage 1 Tunnel F in Hong Kong was carried out as it is a great example of deep sub-sea hard rock TBM tunnelling through unfavourable, fault-affected ground conditions with heavy water inflows. The main objective of this study was to document events that took place during Tunnel F excavation and collate geological and geotechnical data related to the excavation, to aid future tunnel designers and contractors to assess the risk involved with such tunnelling projects. Data relevant to Tunnel F was collected from many personnel involved with the excavation, and from the Hong Kong Geological Survey (HKGS) of the Geotechnical Engineering Office. Geological maps, ground investigation data, tunnel mapping records, ground water inflow records and grouting records were reviewed and used to summarise the ground conditions and to analyse various potential relationships.
Knowledge of ground conditions is the most important requirement for any tunnelling project. This dissertation addresses the differences between actual and predicted ground conditions and the consequences of lack of ground investigation data. The major difference between predicted and observed ground conditions during Tunnel F excavation was the amount of water ingress into the tunnel. Hundreds of liters per minute of water at up to 13bar pressure was encountered from a few probe and grout holes. In the Tolo Channel Fault area, low Q values and very high ground water inflows were experienced, and heavy temporary support and large grout volumes were required to complete the tunnel.published_or_final_version
Applied Geosciences
Master
Master of Science
38
Splitthoff, Daniel Nicolas [Verfasser], and Jürgen [Akademischer Betreuer] Hennig. "SENSE shimming (SSH) : : fast detection of B0 field inhomogeneities in magnetic resonance imaging = SENSE Shimming (SSH) : schnelle Detektion von B0 Feldinhomogenitäten in der Magnet-Resonanz-Bildgebung." Freiburg : Universität, 2012. http://d-nb.info/1123467404/34.
Full text
39
Orlando, Giulia. "Molecular mechanisms of ARF regulation in response to DNA damage." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:fde8ab19-bc7f-4ad8-8396-6c5f5f385d34.
Full textAbstract:
DNA is a highly unstable molecule. Endogenous souces of DNA damage, such as reactive oxygen species (ROS), can cause DNA damage and it has been estimated that 20000 lesions occur in a cell per day. BER is the major pathway for the repair of these lesions and therefore maintains genome stability, thus preventing the development of human diseases such as neurodegenerative diseases and cancer. Therefore, if BER cannot accomplish the repair, accumulation of DNA damage occurs, triggering different cellular responses, such as cell cycle delay and senescence. The ARF tumour suppressor protein, the gene of which is frequently mutated in many human cancers, plays an important role in the cellular stress response by orchestrating upregulation of p53 protein. Moreover, ARF expression is upregulated in senescent cells, suggesting that ARF induction might be triggerred in response to persistent DNA damage. Although ARF has been reported to be important in the regulation of proteins involved in the DNA damage response, its role is still controversial. Here, it has been shown that ARF gene transcription is induced by DNA strand breaks (SBs) and that ARF protein accumulates in response to persistent DNA damage generated by disabling BER. These data suggest that PARP1-dependent poly(ADP-ribose) synthesis at the sites of SBs initiates DNA damage signal transduction by reducing the cellular concentration of NAD+, thus inhibiting SIRT1 activity and consequently activating E2F1-dependent ARF transcription. These findings suggest a vital role for ARF in DNA damage signalling, and furthermore explain the critical requirement for ARF inactivation in cancer cells, which are frequently deficient in DNA repair and accumulate DNA damage.
40
Garin, Etienne. "Développement du 188Re-SSS lipiodol pour le traitement des carcinomes hépatocellulaires." Rennes 1, 2004. http://www.theses.fr/2004REN1B084.
Full textAbstract:
Nous décrivons une moriginale, simple et reproductible de marquage du lipiodol au technétium 99m et au rhénium 188 pour la radiothérapie métabolique des carcinomes hépatocellulaires. La procédure de marquage repose sur la synthèse d'un complexe lipophile du technétium 99m ou du rhénium 188 (le complexe métal-SSS) secondairement solubilisé dans du lipiodol. Le 99mTc-SSS lipiodol obtenu avec une PCR et un rendement de marquage élevés (respectivement 92 et 96%). Stable in vitro au moins 24 heures. Ns avons vérifié chez le porc sain, que le 99mTc-SSS lipiodol était stable et qu'il se fixait de façon très sélective au niveau du foie, après injection au niveau de l'artère hépatique. Le 188Re-SSS lipiodol obtenu à basse activité avec une PCR et un rendement élevés (respectivement 94 et 97%). Stable in vitro au moins 48 heures. Ns avons vérifié, après injection au niveau de l'artère hépatique chez le porc sain et le rat porteur d'un CHC, que le 188Re-SSS lipiodol se fixait préférentiellement au niveau du foie et se concentrait dans la tumeur avec un rapport de fixation "tumeur/foie non tumoral" voisin de 4. Nous avons mis en évidence une élimination urinaire et digestive très faible du 188Re-SSS lipiodol à l'inverse de ce qui a été décrit antérieurement avec d'autres marquages du lipiodol à l'iode 131 et au rhénium 188. Ce point témoigne d'une meilleure stabilité in vitro du 188Re-SSS lipiodol. Une étude de survie avec l'utilisation d'un mélange 188Re-SSS lipiodol/131i-lipiodol, de 188Re-SSS lipiodol seul et de 131i-lipiodol seul chez le rat porteur d'un CHC a été réalisée. Avec un recul de 6 mois elle n'a pas permis de mettre en évidence de supériorité du mélange 188Re-SSS lipiodol/131i-lipiodol ni d'efficacité significative du 188Re-SSSlipiodol. Les résultats de cette étude font évoquer une efficacité limitéé du 188Re-SSS lipiodol sur les lésions de relativement petite taille.
41
Kälkäinen, J. (Juha). "Collection and analysis of malicious SSH traffic in Oulu University network." Bachelor's thesis, University of Oulu, 2018. http://urn.fi/URN:NBN:fi:oulu-201812053224.
Full textAbstract:
Secure Shell (SSH) is a commonly used tool by many organizations to establish secure data communication and remote access to systems that store confidential information and resources. Assessing, defending against and studying the different threats the systems using this protocol are subjected to can be done by using a honeypot. This thesis studied malicious SSH traffic directed at Oulu university network by using an SSH honeypot Cowrie. The honeypot was deployed in the panOULU network located at the Oulu University campus. Two other identical honeypots were deployed from different networks to study if there are differences in malicious traffic directed to all three honeypots. Additionally, a passive fingerprinting tool p0f was used for differentiating what operating systems the attacks originated from. The honeypots successfully remained active and collected data for 12 days. Based on gathered data malicious SSH traffic on all three networks was quite similar. Most common interaction on all three honeypots was likely from bots seeking to exploit common vulnerabilities to gain access to resources. Data from p0f revealed that most common successfully fingerprinted operating system used by attackers on all three honeypots was Linux based. The research showed that honeypots can be used to collect data that is valuable to any security researcher or network administrator. The university network and the other two networks that were studied are constantly subjected to multitude of attacks and scansSecure Shell (SSH) on monissa organisaatioissa yleisesti käytetty työkalu suojatun tietoliikenteen muodostamiseen ja luotettavaa tietoa sekä resursseja sisältävien järjestelmien etäkäyttöön. Tähän protokollaan kohdistettujen eri uhkien arvioimiseen, niitä vastaan puolustautumiseen ja niiden opiskeluun voidaan käyttää hunajapurkkia. Tämä opinnäytetyö tutki Oulun yliopiston verkkoon kohdistettua haitallista SSH-tietoliikennettä käyttämällä SSH-hunajapurkkia Cowrie. Hunajapurkki asetettiin panOULU-verkkoon, joka sijaitsee Oulun yliopiston kampuksella. Kaksi muuta identtistä hunajapurkkia asetettiin eri verkkoihin haitallisen tietoliikenteen poikkeavuuksien tutkimiseksi. Tämän lisäksi passiiviseen sormenjälkitunnistukseen pohjautuvaa työkalua p0f käytettiin selvittämään, mistä eri käyttöjärjestelmistä hyökkäykset alun perin saapuivat. Hunajapurkit olivat onnistuneesti päällä ja keräsivät tietoja 12 päivää. Kerättyjen tietojen perusteella kaikkiin kolmeen verkkoon kohdistettu haitallinen SSH-tietoliikenne oli hyvin samankaltaista. Yleisin kaikkiin kolmeen hunajapurkkiin kohdistettu kanssakäyminen saapui todennäköisesti botteilta, joiden tavoitteena on saada haltuun resursseja hyväksikäyttämällä yleisiä haavoittuvuuksia. P0f-ohjelmalla kerätty tieto paljasti yleisimmän hyökkääjien käyttämän tunnistetun käyttöjärjestelmän olevan Linux-pohjainen. Tutkimus osoitti, että SSH-hunajapurkkeja voidaan käyttää tiedon keräämiseen, joka on arvokasta kenelle tahansa tietoturva-asiantuntijalle tai verkon ylläpitäjälle. Yliopiston verkko ja kaksi muuta tutkittua verkkoa ovat jatkuvan hyökkäyksien ja tarkkailun alaisena
42
Dernaika, Maher. "Molecular Characterization Of Strawberry By Applying Dna Fingerprinting Technique Using Simple Sequence Repeats (ssrs) Markers." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/2/12611258/index.pdf.
Full textAbstract:
In this study, strawberry fruit was taken as the studied model. An attempt was carried on trying to identify a unique DNA fingerprint in each of the selected different strawberry cultivars of Fragaria x ananassa Duch species available in Turkey. The basis of the study was to examine the fruit characteristics at the molecular level rather than at the morphological level. It is of great importance to differentiate and trace the origin of any variety by examining its DNA by using a very sophisticated molecular technique. In this case, DNA fingerprinting technique depending on the Simple Sequence Repeats (SSRs) markers which are also called Microsatellite markers were used. DNA fingerprinting technique reveals the specific DNA profile which is unique as a fingerprint for a fruit specimen and this DNA profile is the same and constant throughout different parts of the fruit as well as its developmental stages. In this thesis work, nine primers flanking the SSR markers already available in the online databases were designed hoping to detect SSRs that could differentiate among the five selected cultivars of strawberry.
43
Masters, Seth Lucian. "The role of the SPRY domain in the SPRY domain containing SOCS box proteins (SSBs) /." Connect to thesis, 2005. http://eprints.unimelb.edu.au/archive/00001571.
Full textAbstract:
Thesis (Ph.D.)--University of Melbourne, The Walter and Eliza Hall Institute of Medical Research, Division of Cancer and Haematology, Dept. of Medical Biology,Faculty of Medicine,Dentistry and Health Sciences, 2006.Typescript. Includes bibliographical references (leaves 192-210).
44
Oliveira, Karine Miranda. "Desenvolvimento de marcadores moleculares EST-SSRs e mapeamento funcional em cana-de-açucar (Saccharum spp.)." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316466.
Full textAbstract:
Orientadores: Anete Pereira de Souza, Antonio Augusto Franco GarciaTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-08T00:10:19Z (GMT). No. of bitstreams: 1 Oliveira_KarineMiranda_D.pdf: 6932327 bytes, checksum: 92b9fb104f8543695a1c804fd65bf912 (MD5) Previous issue date: 2006
Resumo: A cana-de-açúcar (Saccharum spp.) está entre as espécies de maior importância econômica no mundo, constituindo uma das principais fontes de produção de açúcar e álcool. Apesar do Brasil ocupar posição de destaque, como o maior produtor mundial, os níveis de produtividade são considerados baixos. Os programas de melhoramento para obtenção de novas variedades de cana-de-açúcar, mais produtivas e resistentes a pragas e doenças, podem ser acelerados com o desenvolvimento de marcadores genéticos, PCR específicos, fortemente ligados a genes que controlam as características de interesse. A utilização de marcadores em estudos de mapeamento genético e de QTL¿s (Quantitative Trait Loci) tem proporcionado um importante progresso no conhecimento da estrutura genômica e na genética da cana-de-açúcar. Sabe-se que os ESTs (Expressed Sequence Tags) apresentam grande potencial para serem utilizados com tais finalidades. O projeto de seqüenciamento de ESTs (SUCEST), do programa Genoma da FAPESP, identificou cerca de 43 mil clusters que representam os genes de cana-de-açúcar, potenciais para serem utilizados no desenvolvimento de marcadores genéticos. Neste contexto, o presente trabalho teve como objetivo desenvolver e mapear marcadores EST-SSRs em uma progênie derivada do cruzamento entre duas variedades pré-comerciais de cana-de-açúcar, complementando os programas de mapeamento genético desta população. Uma busca no banco de dados do SUCEST resultou na identificação de marcadores microssatélites ou SSRs (Single sequence repeats) em 2005 clusters. Trezentos e setenta e dois locos EST-SSRs foram desenvolvidos e analisados e, destes, 149 foram selecionados para estudo de mapeamento genético. Um total de 2303 marcadores polimórficos (SSRs, EST-SSRs, RFLPs, EST-RFLPs e AFLPs) foi identificado nos 100 indivíduos da progênie F1, dos quais 1669 (72%) eram marcadores em dose simples (1:1 e 3:1), segregantes na população. As análises de mapeamento foram baseadas na metodologia de identificação de marcadores em dosagem única no genoma, com o auxílio dos programas JoinMap (versão 3.0) e OneMap. Para a formação dos grupos de co-segregação (GCs) utilizou-se LOD 5 e fração de recombinação de 0.35. A função de mapeamento de Kosambi foi adotada para conversão das freqüências de recombinação em distâncias de mapa em centiMorgans (cM). Dos 1669 marcadores segregantes, 664 (40%) foram distribuídos em 192GCs, gerando um mapa com 6261.1 cM de comprimento. Os 192 GCs foram agrupados em 14 prováveis grupos de homologia. Cento e treze dos 149 EST-SSRs avaliados foram mapeados e apresentaram homologia a genes conhecidos de outras espécies. A adição dos marcadores provenientes de seqüências expressas, aumentou a cobertura e densidade do mapa prévio desta população, possibilitando também a construção do primeiro mapa funcional de cana-de-açúcar. Os EST-SSRs desenvolvidos têm potencial de utilização na detecção de QTL¿s associados a características de importância econômica para a cultura da cana-de-açúcar
Abstract: Sugarcane (Saccharum spp.) is one of the most important cash crops, highly contributing towards the production of raw sugar and bioethanol produced worldwide. Even though Brazil is the major producer, sugar yield is considered low. Breeding programs for the attainment of new improved sugarcane varieties, which are more productive and more resistant to plagues and illnesses, could be sped up with the development of genetic markers that are PCR-specific and strongly linked to genes that control the desired agronomic traits. The use of genetic markers in genetic mapping and QTL (Quantitative Trait Loci) studies has allowed important progress in the knowledge of the genomic structure and genetics of sugarcane. It is a known fact that the ESTs (Expressed Sequence Tags) have great potential to be used with such purposes. The Sugarcane Expressed Sequence Tag Project (SUCEST) has identified about 43000 clusters that represent the sugarcane genes with a potential to be used in the development of genetic markers. In this context, the objective of the present work was to develop and map EST-SSR markers in a progeny obtained by the cross between two pre-commercial sugarcane varieties, complementing the genetic mapping programs of this population. A search in the SUCEST database resulted on the identification of the microssatellites or SSR (Single sequence repeats) markers in 2005 clusters. Thus, three hundred and seventy two EST-SSR loci had been developed and analyzed and, out of these, 149 were selected for mapping analyses. A total of 2303 polymorphic markers (SSRs, EST-SSRs, RFLPs, EST-RFLPs and AFLPs) were identified among the 100 F1 individuals, of which 1669 (72.5%) were single dose (SD ¿ segregated 1:1 and 3:1) markers. Map analyses were carried out using JoinMap (version 3.0) and OneMap algorithm, based on a single-dose marker approach. The linkage relationships of simplex markers were determined at a LOD score threshold of 5 and a recombination fraction threshold of 0.35 and map distances were derived from the recombination fraction using the Kosambi function. Out of these 1669 SD markers, 664 (40%) were scattered onto 192 co-segregation groups (CGs) with a total estimated map length of 6261.1 cM. Using both genomic and EST-derived SSR and RFLP, 120 of the 192 CGs were formed into fourteen putative homology groups (HGs). One hundred and thirteen of the 149 EST-SSRs evaluated were mapped and presented homology to previously studied genes of other species. The addition of the EST-derived markers increased the coverage and density of the previous map of this population, which also enabled the construction of the first functional linkage sugarcane map. The EST-SSRs developed have the potential to be used in the detection of QTLs associated to important economic traits for sugarcane culture
Doutorado
Genetica Vegetal e Melhoramento
Doutor em Genetica e Biologia Molecular
45
Schuck, Mariane Ruzza. "Caracterização molecular de variedades de videira (VITIS spp.) de Santa Catarina por marcadores microssatélites (SSRs)." Florianópolis, SC, 2007. http://repositorio.ufsc.br/xmlui/handle/123456789/90280.
Full textAbstract:
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-Graduação em Recursos Genéticos Vegetais.Made available in DSpace on 2012-10-23T08:02:20Z (GMT). No. of bitstreams: 1 246930.pdf: 1667453 bytes, checksum: 9c6854327742d0448d006ae863e79f08 (MD5)
A videira é a frutífera economicamente mais importante no mundo e é cultivada para que seus frutos produzam uvas de mesa, suco, uvas passas e vinhos. A espécie Vitis vinifera L. é cultivada pelo mundo todo e muitas variedades importantes têm sido selecionadas durante os séculos. O inventário das variedades de videira descritas na literatura revela a existência de mais de 14000 variedades. Esta grande diversidade genética foi originada pela antiga prática da propagação vegetativa. A dispersão das variedades de videira ou populações de diferentes origens por meio da migração e do comércio conduziu a um grande número de sinônimos, homônimos e ambigüidades na identificação das variedades. Deste modo, a análise genética molecular, baseada no polimorfismo de DNA detectado em locos marcadores tem sido útil no estudo de diversidade genética das coleções de germoplasma facilitando a classificação correta de amostras, bem como a identificação de sinônimos e homônimos. A coleção de germoplasma de videira do Estado de Santa Catarina mantém plantas de videiras que necessitam ser identificadas e caracterizadas corretamente a fim de evitar duplicatas ou sinonímias. Este trabalho objetivou caracterizar e identificar as variedades de videira, das coleções públicas e privadas de Santa Catarina, por marcadores microssatélites. Foram utilizados cinco painéis de marcadores microssatélites (multiplex-seqüenciador) constituídos por primers marcados com fluorocromos para a genotipagem semi-automática em seqüenciador de DNA de 246 amostras de videira, coletados em 7 regiões do Estado de Santa Catarina. Dez locos marcadores distribuídos no genoma da videira foram genotipados utilizando os painéis multiplex e os dados utilizados para identificar variedades sinônimas, de origem desconhecida e erros de identificação, e criar um banco de dados referencial informatizado das variedades de videira (Vitis spp.) de Santa Catarina. Das 246 amostras genotipadas, 181 foram identificadas com segurança e o restante (n=65) por terem amplificado em menos de 6 locos SSRs, não foram identificadas, pois pouco ou nenhum dado referente ao tamanho dos alelos foi suficiente para identifica-lás com segurança. A análise com microssatélites das 181 amostras identificadas resultou em 68 perfis moleculares únicos (68 variedades diferentes), 10 casos de variedades sinônimas (entre as variedades coletadas em Santa Catarina quando comparadas com aquelas encontradas nos 'database' e literatura internacional), identificação de 10 amostras de origem genética desconhecida, 7 casos de erro de identificação e 7 casos de variabilidade intravarietal. Um grande número de alelos pôde ser detectado, o que comprovou a natureza multialélica deste tipo de marcador e a alta eficiência na caracterização e discriminação do germoplasma de Vitis sp. a nível de variedade, gerando fingerprintings únicos para um grande número de genótipos. A metodologia de genotipagem utilizando painéis de microssatélites marcados com fluorescência e genotipados em seqüenciador automático de DNA apresentada mostrou ser um procedimento eficiente, rápido e simples na detecção de polimorfismo SSRs.
46
Leufvén, Claes. "Detecting SSH identity theft in HPC cluster environments using Self-organizing maps." Thesis, Linköping University, Department of Electrical Engineering, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-6818.
Full textAbstract:
Many of the attacks on computing clusters and grids have been performed by using stolen authentication passwords and unprotected SSH keys, therefore there is a need for a system that can detect intruders masquerading as ordinary users. Our assumption is that an attacker behaves significantly different compared to an ordinary user. Previous work in this area is for example statistical analysis of process accounting using Support Vector Machines. We can formalize this into a classification problem that we will solve with Self-organizing maps. The proposed system will work in a tier model that uses process accounting and SSH log messages as data sources.
47
Flores, Kristin K. "Latino student perceptions of TRiO/SSS program effectiveness at Eastern Illinois University /." View online, 2008. http://repository.eiu.edu/theses/docs/32211131400059.pdf.
Full text
48
Watson, Gaven James. "Provable security in practice : Analysis of SSH and CBC mode with padding." Thesis, Royal Holloway, University of London, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.530797.
Full text
49
Romanek, Cristiane. "MAPEAMENTO DE LOCOS DE RESISTÊNCIA QUANTITATIVA À ANTRACNOSE FOLIAR EM MILHO TROPICAL." UNIVERSIDADE ESTADUAL DE PONTA GROSSA, 2014. http://tede2.uepg.br/jspui/handle/prefix/2271.
Full textAbstract:
Made available in DSpace on 2017-07-25T19:30:51Z (GMT). No. of bitstreams: 1
Cristiane Romanek.pdf: 2518407 bytes, checksum: a9fd5d1b1e96d98a44dfc90dfaa0a4b6 (MD5)
Previous issue date: 2014-12-12Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
The objectives of this study were to characterize the resistance/susceptibility of the F2:3 maize progenies to anthracnose leaf blight (Colletotrichum graminicola), to estimate the genetic parameters associated with resistance, and map the genomic regions associated with quantitative resistance loci (QRL) by means molecular markers microsatellites (SSRs) and AFLPs. The inbred lines L04-2 (resistant) and L95-1 (susceptible) were crossed to generate an F2 population. Individuals of this population were selfed resulting F2:3 progenies, these were evaluated in three trials conducted in a randomized block design, with three replications. The treatments were consisted of 121 F2:3 progenies, parental lines and F1 generation. Plants were inoculated in two times between the stages V6 and V7, the second inoculation made seven days after the first. Three evaluation of the severity of anthracnose leaf blight were conducted: the 1st in VT (flowering) stage, 2nd in R2 and 3rd in R3 through a note scale with amplitude from 1 to 6. From the point assessments, was calculated the area under the disease progress curve (AUDPC). The results showed highly significant effects for both point assessments for AUDPC, indicating high genetic variability for resistance to anthracnose leaf blight among the F2:3 progenies. The high magnitude of genetic parameters indicate that the genetic control of resistance to anthracnose leaf blight of corn is associated with the expression of genes of great phenotypic effect. QRL mapping was conducted based on phenotypic analysis of 121 F2:3 progenies, evaluated in three experiments for three point assessments and AUDPC. Linkage analysis between the markers (microsatellites and AFPLs) and QRLs was performed by analysis of multiple linear regression and by composite interval mapping. Multiple linear regression identified mark SSRs and AFPLs highly associated with resistance alleles, the E55139 and E56386 AFLPs loci with the highest partial regression coefficients for the three experiments, with amplitudes from 6,67 to 31,31 % and 6,12 to 21,78 %, respectively. Were mapped 19 QRLs to anthracnose leaf blight in eight linkage groups. Six QRLs were the most stable environments for different evaluation and responsible for the large magnitude of the phenotypic variation in resistance. The high number of QRLs mapped in this population confirms the pattern of quantitative inheritance of resistance in tropical maize to anthracnose leaf blight caused by C. graminicola.
Os objetivos deste trabalho foram caracterizar a resistência/suscetibilidade de progênies F2:3 de milho à antracnose foliar (Colletotrichum graminicola), estimar os parâmetros genéticos associados à resistência, e mapear as regiões genômicas associadas à resistência quantitativa (QRL) por meio de marcadores moleculares microssatélites (SSRs) e AFLPs. As linhagens endogâmicas L04-2 (resistente) e L95-1 (suscetível) foram cruzadas entre si a fim de gerar uma população F2. Os indivíduos desta população foram autofecundados dando origem as progênies F2:3 que foram avaliadas em três experimentos, conduzidos no delineamento de blocos casualizados com três repetições. Os tratamentos foram constituídos de 121 progênies F2:3, linhagens parentais e geração F1. As plantas foram inoculadas por duas vezes entre os estádios V6 e V7, sendo a 2ª inoculação realizada sete dias após a 1ª. Foram realizadas três avaliações da severidade da antracnose foliar: a 1ª no estádio VT (pendoamento), a 2ª em R2 e a 3ª em R3 através de uma escala de notas com amplitude de 1 a 6. A partir das avaliações pontuais, calculou-se a área abaixo da curva de progresso da doença (AACPD). Os resultados evidenciaram efeitos altamente significativos tanto para as avaliações pontuais quanto para a AACPD, indicando grande variabilidade genética para a resistência à antracnose foliar entre as progênies F2:3. As elevadas magnitudes dos parâmetros genéticos indicam que o controle genético da resistência de milho à antracnose foliar esteja associado à expressão de genes de grande efeito fenotípico. O mapeamento de QRLs foi realizado com base na análise fenotípica das 121 progênies F2:3, avaliadas em três experimentos para as três avaliações pontuais e para a AACPD. A análise de ligação entre os marcadores (microssatélites e AFPLs) e os QRLs foi efetuada por meio da análise de regressão linear múltipla e pelo mapeamento por intervalo composto. A regressão linear múltipla identificou marcas SSRs e AFPLs altamente associadas a alelos de resistência, sendo os locos AFLPs E55139 e E56386 com os maiores coeficientes de regressão parciais para os três experimentos, com amplitude de 6,67 a 31,31 % e de 6,12 a 21,78 %, respectivamente. Foram mapeados 19 QRLs à antracnose foliar em 8 grupos de ligação. Seis QRLs foram mais estáveis para os diferentes ambientes de avaliação e responsáveis por grande magnitude da variação fenotípica em resistência. O elevado número de QRLs mapeados nesta população confirma o padrão de herança quantitativa da resistência de milho tropical à antracnose foliar causada por C. graminicola.
50
Santana, Ariane Rolins de. "Transferibilidade e desenvolvimento de sistema multiplex de genotipagem de marcadores microssatélites para Pterodon emarginatus Vogel (Fabaceae)." Universidade Federal de Goiás, 2014. http://repositorio.bc.ufg.br/tede/handle/tede/4501.
Full textAbstract:
Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-05-08T15:19:29Z
No. of bitstreams: 2
Dissertação - Ariane Rolins de Santana - 2014.pdf: 1616629 bytes, checksum: 564437916d584de9d3769067e398b29b (MD5)
license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-05-08T15:37:12Z (GMT) No. of bitstreams: 2 Dissertação - Ariane Rolins de Santana - 2014.pdf: 1616629 bytes, checksum: 564437916d584de9d3769067e398b29b (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)
Made available in DSpace on 2015-05-08T15:37:12Z (GMT). No. of bitstreams: 2 Dissertação - Ariane Rolins de Santana - 2014.pdf: 1616629 bytes, checksum: 564437916d584de9d3769067e398b29b (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-04-10
Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG
Microsatellite markers are widely used to assess the genetic variability in natural populations. These markers can be developed from derived Expressed sequence tag (ESTs) or genomic libraries. A feasible and economical alternative for obtaining of these markers for species that do not already have them is the transferability from primers designed for species evolutionarily close. The species Pterodon emarginatus Vogel (sucupira) belongs to the Fabaceae family and shows great potential for use timber and medicinal, which makes them a target of exploitation. Thus, studies that contribute to their use and conservation are needed, including the genetic population. Therefore, the aim of this study was to evaluate the potential for cross-amplification primers developed for the species Phaseolus vulgaris to P. emarginatus and to analyze the polymorphism of the loci transferred from multiplex genotyping in three populations of this species. For this, were evaluated 539 primers developed for Phaseolus vulgaris, with 345 derived from ESTs libraries and 194 genomics. Amplification tests were performed using three individuals, while the assessment initial of the polymorphism with eight. Finally, genetic diversity parameters were estimated using 88 plants from three natural populations of P. emarginatus. Primers that showed amplification products visualized on agarose gel were then evaluated in 6% acrylamide gel stained with silver nitrate. Polymorphic loci were synthesized with fluorescence for evaluation and genotyping by capillary electrophoresis in ABI3500 automatic DNA analyzer. The loci were transferred to P. emarginatus 23 (4 %) being 7 polymorphic. Polymorphic, 6 electropherograms profiles. These 6 loci were evaluated in populations along with three polymorphic genomic loci previously standardized sucupira, totaling nine loci suitable for genotyping. It was possible to develop two multiplex systems, a compound of 5primers and another for 4. The number of alleles per locus ranged from two to 13, with an average of five alleles per loci. The mean values of He and Ho for both populations were 0,563 and 0,463 respectively. The value of f was not significant for the total population and equal to 0,144. The values FIT and FST were significant and equal to 0,06 and 0,195 respectively. Microsatellite markers transferred and polymorphic for P. emarginatus showed polymorphism and can be used in studies genetic population more depth to the species.
Os marcadores microssatélites são amplamente utilizados para acessar a variabilidade genética em populações naturais. Estes marcadores podem ser desenvolvidos a partir de bibliotecas derivadas de Sequências Expressas Transcritas (ESTs) ou genômicas. Uma alternativa possível e econômica para a obtenção destes marcadores para espécies que ainda não os possuem, é a transferibilidade a partir de primers desenhados para espécies evolutivamente próximas. A espécie Pterodon emarginatus Vogel (sucupira) pertence à família Fabaceae e apresenta grande potencial de uso medicinal e madeireiro, o que faz dela alvo da exploração extrativista. Assim, estudos que contribuam com o seu melhor uso e conservação são necessários, dentre eles os genético-populacionais. Diante disso, o objetivo deste estudo foi avaliar o potencial de amplificação cruzada de primers desenvolvidos para a espécie Phaseolus vulgaris para P. emarginatus e analisar o polimorfismo dos locos transferidos a partir de sistema multiplex de genotipagem em três populações desta espécie. Para tanto, foram avaliados 539 primers desenvolvidos para Phaseolus vulgaris, sendo 345 derivados de bibliotecas de ESTs e 194 genômicos. Os testes de amplificação foram realizados utilizando três indivíduos, enquanto a avaliação inicial do polimorfismo com oito. Por fim, os parâmetros genéticos de diversidade foram estimados utilizando 88 plantas, provenientes de três populações naturais de P. emarginatus. Os primers que apresentaram produtos de amplificação visualizados em gel de agarose foram, posteriormente, avaliados em gel de acrilamida 6% corado com nitrato de prata. Os locos polimórficos foram sintetizados com fluorescência para a avaliação e genotipagem via eletroforese capilar, no analisador automático de DNA ABI3500. Os locos transferidos para P. emarginatus foram 23 (4%) sendo 7 polimórficos. Dos polimórficos, 6 apresentaram bons perfis de eletroferogramas. Estes 6 locos foram avaliados nas populações juntamente com três locos genômicos polimórficos previamente padronizados para sucupira, totalizando nove locos adequados para genotipagem. Foi possível o desenvolvimento de dois sistemas multiplex, um composto de 5 primers e outro por 4. O número de alelos por loco variou de dois a 13, com uma média de cinco alelos por loco. Os valores médios de He e Ho para o conjunto das populações foram de 0,563 e 0,463 respectivamente. O valor de f não foi significativo para o total das populações e igual a 0,144. Os valores de FST e FIT foram significativos e iguais a 0,06 e 0,195 respectivamente. Os marcadores microssatélites transferidos e polimórficos para P. emarginatus apresentaram bom polimorfismo e podem ser utilizados em estudos genético-populacionais mais aprofundados com a espécie.