Journal articles on the topic 'SRFΔ5'
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Belaguli, Narasimhaswamy S., Wei Zhou, Thuy-Hanh T. Trinh, Mark W. Majesky, and Robert J. Schwartz. "Dominant Negative Murine Serum Response Factor: Alternative Splicing within the Activation Domain Inhibits Transactivation of Serum Response Factor Binding Targets." Molecular and Cellular Biology 19, no. 7 (July 1, 1999): 4582–91. http://dx.doi.org/10.1128/mcb.19.7.4582.
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ABSTRACT Primary transcripts encoding the MADS box superfamily of proteins, such as MEF2 in animals and ZEMa in plants, are alternatively spliced, producing several isoformic species. We show here that murine serum response factor (SRF) primary RNA transcripts are alternatively spliced at the fifth exon, deleting approximately one-third of the C-terminal activation domain. Among the different muscle types examined, visceral smooth muscles have a very low ratio of SRFΔ5 to SRF. Increased levels of SRFΔ5 correlates well with reduced smooth muscle contractile gene activity within the elastic aortic arch, suggesting important biological roles for differential expression of SRFΔ5 variant relative to wild-type SRF. SRFΔ5 forms DNA binding-competent homodimers and heterodimers. SRFΔ5 acts as a naturally occurring dominant negative regulatory mutant that blocks SRF-dependent skeletal α-actin, cardiac α-actin, smooth α-actin, SM22α, and SRF promoter-luciferase reporter activities. Expression of SRFΔ5 interferes with differentiation of myogenic C2C12 cells and the appearance of skeletal α-actin and myogenin mRNAs. SRFΔ5 repressed the serum-induced activity of the c-fos serum response element. SRFΔ5 fused to the yeast Gal4 DNA binding domain displayed low transcriptional activity, which was complemented by overexpression of the coactivator ATF6. These results indicate that the absence of exon 5 might be bypassed through recruitment of transcription factors that interact with extra-exon 5 regions in the transcriptional activating domain. The novel alternatively spliced isoform of SRF, SRFΔ5, may play an important regulatory role in modulating SRF-dependent gene expression.
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Gerosa, Laura, Barbara Grillo, Chiara Forastieri, Alessandra Longaretti, Emanuela Toffolo, Alessandra Mallei, Silvia Bassani, Maurizio Popoli, Elena Battaglioli, and Francesco Rusconi. "SRF and SRFΔ5 Splicing Isoform Recruit Corepressor LSD1/KDM1A Modifying Structural Neuroplasticity and Environmental Stress Response." Molecular Neurobiology 57, no. 1 (July 30, 2019): 393–407. http://dx.doi.org/10.1007/s12035-019-01720-8.
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Riana, Eri. "Penerapan Sensor Ultrasonic SRF05 Berbasis Mikrocontroller ATMega 8535 Untuk Sistem Pengereman Otomatis." Journal of Information System Research (JOSH) 2, no. 4 (July 25, 2021): 268–75. http://dx.doi.org/10.47065/josh.v2i4.761.
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Transportation technology using electric energy is growing. Electric cars are increasingly being used to reduce fuel oil usage. In addition to the increasingly sophisticated performance of electric cars, the vehicle safety system must also be improved. The existence of Artificial Intelligence is one of the developments in technological sophistication in a world that is increasingly unstoppable and unavoidable. One of the most popular luxury cars in Indonesia, one of which is the Tesla, which is a sophisticated car with the support of an artificial intelligence system, making Tesla an electric car with an automatic control vehicle feature that can run without using a human driver and can provide safety protection for the driver by using automatic braking system in case of an unexpected accident. This journal proposes a future concept design for the forerunner of automatic braking for electric cars using the ATMEGA 8535 microcontroller as the main automatic control and ultrasonic distance sensor. The PWM signal from the microcontroller is used to adjust the speed of the DC motor as the main driving force of the vehicle. The display of the distance between the vehicle and the obstacle can be seen on the LCD and the buzzer is used as an indicator of the safe distance of the car. The experiment was carried out using an RC car with the area to be protected> 500cm (5m), if the SRF05 ultrasonic sensor detects an obstacle at that distance, the SRF05 ultrasonic sensor will send a signal to the microcontroller and turn on an alarm in the form of a buzzer so that the SRF05 ultrasonic sensor will stop signal propagation.
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Putri Husain, Nursuci, Herlinah B, Mithahul Khairah, and M. Akbar. "Rancang Bangun Pendeteksi Jarak Aman Mata Dengan Layar Monitor Televisi Berbasis Arduino Uno." Jurnal Teknologi dan Komputer (JTEK) 2, no. 02 (December 31, 2022): 184–89. http://dx.doi.org/10.56923/jtek.v2i02.96.
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Mata adalah indera penglihatan yang berfungsi mempersepsikan bentuk, ukuran, maupun kedudukan suatu objek. Menonton layar televisi dengan jarak 0–119 cm, dapat menyebabkan kerusakan pada mata seperti mata minus, mata juling dan penyempitan pembuluh darah. Paparan dari cahaya televisi akibat menonton terlalu dekat akan berefek pada mata seperti sakit kepala, mata rabun, mata perih, mata kering dan mata berair. Tujuan penelitian merancang bangun pendeteksi jarak aman mata dengan layar monitor televisi berbasis arduino uno guna mencegah menonton layar televisi terlalu dekat yang mengakibatkan kerusakan pada mata. Metode penelitian Research and Development berfungsi menghasilkan, mengembangkan produk tertentu serta menguji keefektifan produk (alat pendeteksi jarak aman mata). Pengujian sistem yang dilakukan adalah pengujian arduino uno, sensor HY-SRF05, LCD, push button, relay, buzzer. Hasil pengujian alat menggunakan arduino uno terdiri dari dua proses yaitu input proses dan output proses, ketika sensor HY-SRF05 mendeteksi jarak objek mata <120 cm maka relay akan memutuskan tegangan terminal selama 10 detik dan televisi OFF, sebaliknya jika jarak objek mata ≥120 cm relay akan meyambungkan tegangan ke terminal dan televisi ON. Kesimpulan sensor pendeteksi objek pada alat pengatur jarak aman mata beroperasi dengan lancar dan akurat.
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Lonteng, Imanuel Yosua, Gunawan Gunawan, and Isa Rosita. "Rancang Bangun Simulasi Alat Pendeteksi Jarak Aman Antar Kendaraan Menggunakan Sensor Ultrasonik Berbasis Arduino." JEECOM: Journal of Electrical Engineering and Computer 2, no. 2 (October 31, 2020): 22–25. http://dx.doi.org/10.33650/jeecom.v2i2.1482.
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Padatnya kendaraan di Indonesia dan semakin banyak nya variasi kendaraan akan cukup menyulitkan pengemudi, terutama pengemudi pemula untuk dapat menjaga jarak secara maksimal oleh karena panjang bumper mobil dari tiap-tiap kendaraan yang cukup bervariasi.Penelitian kali ini diharapkan kedepannya dapat membantu pengemudi untuk dapat menjaga jarak dengan kendaraan didepan, dan mengurangi resiko kecelakaan. Pada penelitian ini penulis mensimulasikan menggunakan mobil simulasi (remote control) dihubungkan dengan sensor ultrasonik HY-SRF05 yang digunakan untuk mendeteksi dan menghitung jarak terhadap objek, lalu hasil jarak tersebut di proses oleh Arduino nano yang selanjutnya apabila kondisi pertama terpenuhi mobil simulasi akan melakukan pengereman, lalu apabila kondisi kedua terpenuhi mobil simulasi akan berhentiMobil simulasi dapat berhenti sesuai dengan jarak yang diharapkan pada simulasi ini. Setelah diamati sensor akan semakin akurat apabila sudut objek penghalang terhadap sensor semakin tegak luru.
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Hanifadinna, Hanifadinna. "Perancangan Water Level Monitoring pada Raw Water Tank dengan Sensor Ultrasonik Berbasis Nodemcu di Sungai Kupang Mill." JURNAL VOKASI TEKNOLOGI INDUSTRI (JVTI) 4, no. 2 (October 31, 2022): 01–08. http://dx.doi.org/10.36870/jvti.v4i2.304.
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Kegiatan monitoring atau pengecekan pada raw water tank di pabrik kelapa sawit Sungai Kupang dilakukan dengan mengandalkan bandul ukur level dan sirine indikator. Kedua alat ini sering mengalami masalah sehingga menjadi kendala dalam kegiatan monitoring. Berdasarkan masalah tersebut maka dibuatlah alat tambahan untuk kegiatan monitoring level air dengan mengandalkan internet sebagai media penghubungnya atau disebut Internet of Things. Bertujuan untuk memudahkan kegiatan monitoring dengan akses secara jarak jauh dan dapat mengirimkan pemberitahuan level rendah pada smartphone penggunanya. Alat ini memanfaatkan konsep pengukuran yaitu suatu kegiatan membandingkan antara besaran dengan besaran acuan dimana tersusun dari beberapa komponen yaitu sensor ultrasonik HY-SRF05 sebagai pendeteksi ketinggian permukaan air, NodeMCU Esp8266 sebagai pemroses data, aplikasi Blynk dan LCD Keypad sebagai penampil data, module datalogger sebagai penyimpan data, serta komponen power supply. Data diperoleh dengan menggunakan metode kuantitatif dan kualitatif untuk mengetahui data yang berupa nominal atau berupa deskripsi. Hasil pengujian alat didapatkan error minimal 0,03% dan maksimal 3,89% dengan interval selisih 0,14 – 8,63 cm. Alat ini dapat memudahkan operator dalam melakukan monitoring level air pada raw water tank dengan akses jarak jauh dan dapat mengirimkan pemberitahuan level rendah serta dapat digunakan sebagai alternatif kegiatan monitoring selain mengandalkan alat yang telah terpasang sebelumnya.
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Mahendra, Andre, and Nini Firmawati. "Rancang Bangun Alat Mosquito Killer Menggunakan Buzzer dan Perangkap Lampu Violet." Jurnal Fisika Unand 12, no. 1 (December 25, 2022): 70–76. http://dx.doi.org/10.25077/jfu.12.1.70-76.2023.
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Telah dihasilkan alat mosquito killer menggunakan buzzer dan perangkap lampu violet. Alat mosquito killer bertujuan untuk membasmi nyamuk dengan gelombang ultrasonik. Penelitian ini membuat alat mosquito killer berbentuk tabung berdimensi (7 x 41) cm, bagian atas tabung berisikan LED violet untuk memikat nyamuk mendekati tabung, kemudian kipas 12 volt pada bagian tengah tabung untuk menghisap nyamuk ke dalam tabung. Bagian bawah tabung terdapat jaring halus sebagai keluarnya aliran udara dan tempat nyamuk terperangkap. Sensor ultrasonik SRF05 mendeteksi objek yang masuk ke dalam tabung, secara otomatis buzzer sebagai aktuator menghasilkan suara berupa gelombang ultrasonik selama 20 detik pada setiap variasi frekuensi. Objek penelitian ini adalah nyamuk, ketika nyamuk masuk ke dalam mosquito killer berjumlah 25 ekor didapatkan hasil percobaan sebagai berikut: pada frekuensi (30 – 40) kHz nyamuk yang mati adalah 0%, pada frekuensi 45 kHz nyamuk yang mati adalah 20%, pada frekuensi 50 kHz nyamuk yang mati adalah 100%, pada frekuensi 55 kHz nyamuk yang mati adalah 100% dan pada frekuensi 60 kHz nyamuk yang mati adalah 100%. Kesimpulan penelitian ini adalah frekuensi gelombang ultrasonik minimal dalam membunuh nyamuk adalah 50 kHz dengan lama waktu 20 detik.
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Suryantoro, Hery. "Prototype Sistem Monitoring Level Air Berbasis Labview dan Arduino Sebagai Sarana Pendukung Praktikum Instrumentasi Sistem Kendali." Indonesian Journal of Laboratory 1, no. 3 (August 13, 2019): 20. http://dx.doi.org/10.22146/ijl.v1i3.48718.
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Air adalah kebutuhan yang sangat penting, ketersediaan air tetap harus selalu ada baik di rumah tangga, perkantoran, tempat-tempat umum ataupun industri. Hal ini menyebabkan peran tangki penampung air fundamental untuk menjamin ketersediaan air. Pengukuran ketinggian air secara otomatis adalah salah satu parameter yang perlu diukur untuk mendeteksi keadaan tangki penampung yang meluap atau kosong. Salah satunya dengan membuat sensor pengukur level air yang diumpanbalikkan menggunakan pompa air. Pada penelitian ini dibuat prototipe dengan menggunakan Arduino Uno R3 dan software LabVIEW sebagai pusat pengolah data yang terintegrasi dengan sensor ultrasonic SRF05 sebagai pembaca ketinggian air. Prototipe ini dilengkapi dengan indikator kondisi air, indikator pompa air yang diatur dari Arduino. Metode yang digunakan pada penelitian ini yaitu perencanaan, pengumpulan bahan, perancangan hardware, pembuatan program Arduino dan LabView, pengujian. Pengambilan data dilakukan dengan mengubah level air dengan membuka kran secara bertahap. Berdasarkan hasil pengamatan didapatkan hasil perbandingan antara level air yang tertera pada tangki penampung air dengan level air yang tertampil pada monitoring Arduino dan LabVIEW yaitu level air penampung 22 cm hasil sistem monitoring 22,12 cm, level air 15,5 cm hasil sistem monitoring 14,47cm, level air 2,5 cm hasil sistem monitoring 2,52 cm. Rata-rata kesalahan pembacaan pengukuran level air sebesar 0,089 cm.
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Ngôn, Nguyễn Chí, Trần Thanh Tân, Văn Công Phụng, and Nguyễn Minh Cảnh. "CẢI THIỆN THIẾT BỊ HỖ TRỢ NGƯỜI KHIẾM THỊ ĐIỀU HƯỚNG DI CHUYỂN DÙNG SÓNG SIÊU ÂM." TNU Journal of Science and Technology 226, no. 11 (August 27, 2021): 292–99. http://dx.doi.org/10.34238/tnu-jst.4812.
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Thiết bị hỗ trợ người khiếm thị đi đường dùng sóng siêu âm và cảnh báo chướng ngại vật bằng tiếng Việt đã được nhóm nghiên cứu triển khai từ năm 2012. Tuy nhiên, thực tế sử dụng cho thấy thiết bị vẫn còn tình trạng cảnh báo sai, do giải thuật kiểm soát cảm biến siêu âm còn hạn chế. Nghiên cứu này tập trung cải thiện giải thuật để khắc phục tình trạng cảnh báo giả. Phần cứng của thiết bị được kế thừa lại, với vi điều khiển 89C2051, các mô-đun cảm biến siêu âm SRF05 và mô-đun cảnh báo tiếng Việt dùng chip ISD1420. Phần mềm hệ thống trên vi điều khiển được tích hợp thêm hàm bù sai số cảm biến dùng giải thuật bình phương tối thiểu Levenberg-Marquardt. Thử nghiệm cho thấy, giải thuật cải tiến có thể đo khoảng cách chướng ngại vật với tỷ lệ chính xác đạt hơn 96%, trong dãy khoảng hữu dụng từ 30 đến 90 cm. Đồng thời thực nghiệm thiết bị tại Hội người mù Thành phố Cần Thơ và Hội người mù huyện Đức Hòa, tỉnh Long An cho thấy, thiết bị đáp ứng được sự mong đợi của cộng đồng người khiếm thị, cũng như mang lại nhiều hi vọng cho họ về một sản phẩm hữu ích giá rẻ.
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Yoeseph, N. M., F. A. Purnomo, R. Hartono, and Nuryani. "Lora-based IoT sensor node for Real-time Flood Early Warning System." IOP Conference Series: Earth and Environmental Science 986, no. 1 (February 1, 2022): 012060. http://dx.doi.org/10.1088/1755-1315/986/1/012060.
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Abstract Internet of Things (IoT) using LoRa technology has been widely used in many fields. One of them is in disaster mitigation and management. This paper presents sensor nodes as part of a Flood Early Warning System using LoRa technology. The node consists of a water level sensor and a water flow meter. Water level measurements were carried out by ultrasonic sensors HY-SRF05, and the speed of water flow is measured by water flow meter YF-S201. Communication between the nodes and the gateway is done by the BsFrance Lora32u4 II v1.4 Wireless Transceiver module. Data regarding the height of the water level and speed of water flow will be measured by the sensor and sent by the LoRa device to the gateway. The gateway then forwards that data to an open-source IoT platform for analysis and visualization. Testing ultrasonic sensor readings get an accuracy of 97.06%. Waterflow sensor testing obtained 91.96% accuracy. In addition to testing the measurement accuracy, data transmission consistency is also tested against the distance between the gateway and the node. The test results show that the data can still be sent at a space between the node and the gateway below 400 m. Above 400m, the data cannot be received by the gateway anymore. Testing delay time between reading data and receiving data on the IoT platform shows the average delivery delay of 0 ms.
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Kris Widiantara, I. Made, Linawati Linawati, and Dewa Made Wiharta. "RANCANG BANGUN AKUAPONIK BERBASIS INTERNET OF THINGS." Jurnal SPEKTRUM 8, no. 1 (March 28, 2021): 243. http://dx.doi.org/10.24843/spektrum.2021.v08.i01.p27.
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Currently technology is growing so that many tools are created, especially reforms inagriculture. Aquaponics is a combination of hydroponic and aquaculture techniques intoaquaponics techniques. This aquaponics technique only uses water as a growing medium.Cultivating aquaponics does not require a large area of land, it can be done in the yard of ahouse that does not have a large area. Currently aquaponics techniques have developed,especially in modern agriculture because this aquaponics technique will use an Internet ofThings (IoT) based system. Internet of Things (IoT) is a concept in which the virtual world of information technology blends with real objects in the real world. In other words, the Internetof Things (IoT) is a concept which aims to expand the benefits of continuously connectedinternet connectivity. This system is built using the Node Mcu Esp8266 microcontroller as thecontrol center and uses the Raspberry Pi 3 type B mini PC as visual notification and is equippedwith a pH sensor 4502C, DS18B20 temperature sensor, Servo Motor, SRF05 sensor. A plantcommonly used in aquaponics is pakcoy, which is one of the most widespread vegetables inAsia. While the fish used in aquaponics techniques are freshwater fish, one of which is parrotfish. In testing the pH sensor is carried out with 3 conditions, namely when the pH is normal,acidic and alkaline on the sensor test detects normal pH, acid and alkaline approaching theresults of the pH meter comparison tool, then the water level test is carried out with 2 conditions,namely when the water is normal and reduced by using a ruler comparison tool, where thesensor has a little detecting error, on testing the water temperature sensor using a thermometercomparator has an error detecting the state of the aquaponic water temperature. When testingthe chat feature is done by typing / getdata, the application will send a chat with sub watertemperature, water level, water pH, then testing the camera feature is done by typing /getpHoto, the application will send a picture of the condition of the aquaponics.
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Pindrayana, Kadek, Rohmat Indra Borman, Bagas Prasetyo, and Samsugi Samsugi. "Prototipe Pemandu Parkir Mobil Dengan Output Suara Manusia Mengunakan Mikrokontroler Arduino Uno." CIRCUIT: Jurnal Ilmiah Pendidikan Teknik Elektro 2, no. 2 (November 4, 2018). http://dx.doi.org/10.22373/crc.v2i2.3705.
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To facilitate the parking of the car to be efficient parking equipment was needed by using the distance with objects. In this research, the development of prototype of car parking guide by displaying information by using centimeter or meter unit and using sound output to facilitate the user in parking their vehicle. From the analysis and testing of prototype car park guides with human voice output indicates that this prototype can give good results. Ultrasonic sensor SRF05 can work well but in the sensor distance of more than 3 meters cannot send data
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Nuryanto, Nuryanto, Andi Widiyanto, and Auliya Burhanuddin. "Redirection Concept of Autonomous Mobile Robot HY-SRF05 Sensor to Reduce The Number of Sensors." Proceeding of the Electrical Engineering Computer Science and Informatics 4, no. 1 (October 1, 2017). http://dx.doi.org/10.11591/eecsi.v4.1056.
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Boimau, Infianto, Rudi Irmawanto, and Marsofran F. Taneo. "Rancang Bangun Alat Ukur Laju Bunyi Di Udara Menggunakan Sensor Ultrasonik Berbasis Arduino." CYCLOTRON 2, no. 2 (August 8, 2019). http://dx.doi.org/10.30651/cl.v2i2.3253.
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Abstrak— Rendahnya kemampuan peserta didik dalam memahami konsep-konsep abstrak dalam proses pembelajaran memerlukan adanya solusi berupa penerapan alat peraga sehingga meningkatkan motivasi dan prestasi belajar. Dalam penelitian ini dikembangkan alat ukur untuk menentukan kelajuan bunyi di udara menggunakan sensor ultrasonik berbasis Arduino. Metode rancang bangun alat ukur terdiri dari perangkat keras (hardware) dan perangkat lunak (sotfware). Rancang bangun alat ukur laju bunyi di udara dikembangkan dengan memanfaatkan gelombang ultrasonik sebesar 40 kHz yang dibangkitkan oleh sensor ultrasonik tipe HY-SRF05. Hasil penelitian menunjukkan bahwa alat ukur yang telah dikembangkan mampu mengukur laju bunyi di udara pada suhu 250C. Hasil pengukuran laju bunyi di udara yang diperoleh menggunakan alat ukur yang dikembangkan adalah sebesar 348,3 m/s dengan persentase error sebesar 2,29%..Kata kunci: laju bunyi, sensor ultrasonik, ArduinoAbstract— Students’ ability which is very low in comprehending the abstract concepts in learning needs a solution in the form of designing learning media that can be used to improve students’ motivation and learning achievements. In this study, a learning media was designed to determine the sound speed on the air using ultrasonic sensor based on Arduino. The methods of the designed learning media consist of hardware and software devices. The media designed for measuring sound speed on the air was improved using 40 𝑘𝐻𝑧 ultrasonic wave which is supported by ultrasonic sensor HY-SRF05. The result of the study showed that the designed media could measure the sound speed on the air on 250C degree. The result of sound speed measurement was gained using the designed media was 348,3 𝑚/𝑠 with error percentage was 2,29%.. Keywords: sound speed, ultrasonic sensor, Arduino
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Xu, Yaxiu, Zhi Liu, Tianxing Lv, Yun Wei, Weiting Liu, Yajing Wei, Guangxin Yang, Li Liu, Tong Li, and Aide Wang. "Exogenous Ca2+ Promotes Transcription Factor Phosphorylation to Suppress Ethylene Biosynthesis in Apple." Plant Physiology, January 19, 2023. http://dx.doi.org/10.1093/plphys/kiad022.
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Abstract Ethylene biosynthesis in apple (Malus domestica) fruit can be suppressed by Calcium ions (Ca2+) during storage; however, the underlying mechanisms are unclear. In this study, we identified the apple transcription factor MCM1-AGAMOUS-DEFICIENS-SRF5 (MdMADS5), which functions as a transcriptional activator of the ethylene biosynthesis-related gene 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASE1 (MdACS1), a partner of the calcium sensor CALCIUM-DEPENDENT PROTEIN KINASES7 (MdCDPK7). Ca2+ promoted the MdCDPK7-mediated phosphorylation of MdMADS5, which resulted in the degradation of MdMADS5 via the 26S proteasome pathway. MdCDPK7 also phosphorylated 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID OXIDASE1 (MdACO1), the key enzyme in ethylene biosynthesis, leading to MdACO1 degradation and inhibition of ethylene biosynthesis. Our results reveal that Ca2+/MdCDPK7-MdMADS5 and Ca2+/MdCDPK7-MdACO1 are involved in Ca2+-suppressed ethylene biosynthesis, which delays apple fruit ripening. These findings provide insights into fruit ripening, which may lead to the development of strategies for extending the shelf life of fruit.
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Zhou, Ting, Mingming Li, Zhijun Xiao, Jian Cai, Weiwei Zhao, Jingjing Duan, Zhen Yang, et al. "Chronic Stress-Induced Gene Changes In Vitro and In Vivo: Potential Biomarkers Associated With Depression and Cancer Based on circRNA- and lncRNA-Associated ceRNA Networks." Frontiers in Oncology 11 (September 28, 2021). http://dx.doi.org/10.3389/fonc.2021.744251.
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Circular RNAs (circRNAs) and long noncoding RNAs (lncRNAs) have been considered as biomarkers or regulators in many diseases. However, the exact role of circRNA- or lncRNA-mediated competing endogenous RNA (ceRNA) networks in the modulation of depression pathogenesis-relevant processes is not clear. In this study, we profiled whole transcriptome in depression patients’ blood samples via microarray analysis. As a result, a total of 340 circRNAs, 398 lncRNAs, 206 miRNAs, and 92 mRNAs were differentially expressed between the depression and control groups. Then, we constructed ceRNA networks according to the differentially expressed genes (DEGs). Using bioinformatics analysis, 89 pairs of circRNA-ceRNA and 49 pairs of lncRNA-ceRNA networks were obtained. Since depression is a broad and heterogeneous condition that is known as promoter for many chronic diseases including cancer, so we further dug out 28 circRNAs, 61 lncRNAs, 26 miRNAs, and 29 mRNAs that are associated with cancer. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that the DEGs were significantly enriched in cancer-related signaling pathways such as MAPK, Wnt, IL-17, Ras, and PI3K-Akt. Genes involved in the above pathways such as S100A9, GATA2, SRFP5, SLC45A3, NTRK1, FRZB, has_circ_0014221, has_circ_0014220, and has_circ_0087100 were dysregulated in various cancer cell lines by stress hormones induced. HDC, GATA2, SLC45A3, and NTRK1 were downregulated in tumor-bearing mice subjected to chronic unpredictable mild stress (CUMS). LncRNA-mediated ceRNA network validation showed that overexpression of miR-4530 declined HDC level. Our findings highlight the potential circRNA- and lncRNA-mediated ceRNA regulatory mechanisms in the pathogenesis of depression and as potential biomarkers in depression cancer comorbidity through the pathways of IL-17 or histidine metabolism.
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Havenga, Benjamin, Thando Ndlovu, Tanya Clements, Brandon Reyneke, Monique Waso, and Wesaal Khan. "Exploring the antimicrobial resistance profiles of WHO critical priority list bacterial strains." BMC Microbiology 19, no. 1 (December 2019). http://dx.doi.org/10.1186/s12866-019-1687-0.
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Abstract Background The antimicrobial resistance of clinical, environmental and control strains of the WHO “Priority 1: Critical group” organisms, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa to various classes of antibiotics, colistin and surfactin (biosurfactant) was determined. Methods Acinetobacter baumannii was isolated from environmental samples and antibiotic resistance profiling was performed to classify the test organisms [A. baumannii (n = 6), P. aeruginosa (n = 5), E. coli (n = 7) and K. pneumoniae (n = 7)] as multidrug resistant (MDR) or extreme drug resistant (XDR). All the bacterial isolates (n = 25) were screened for colistin resistance and the mobilised colistin resistance (mcr) genes. Biosurfactants produced by Bacillus amyloliquefaciens ST34 were solvent extracted and characterised using ultra-performance liquid chromatography (UPLC) coupled to electrospray ionisation mass spectrometry (ESI–MS). The susceptibility of strains, exhibiting antibiotic and colistin resistance, to the crude surfactin extract (cell-free supernatant) was then determined. Results Antibiotic resistance profiling classified four A. baumannii (67%), one K. pneumoniae (15%) and one P. aeruginosa (20%) isolate as XDR, with one E. coli (15%) and three K. pneumoniae (43%) strains classified as MDR. Many of the isolates [A. baumannii (25%), E. coli (80%), K. pneumoniae (100%) and P. aeruginosa (100%)] exhibited colistin resistance [minimum inhibitory concentrations (MICs) ≥ 4 mg/L]; however, only one E. coli strain isolated from a clinical environment harboured the mcr-1 gene. UPLC-MS analysis then indicated that the B. amyloliquefaciens ST34 produced C13–16 surfactin analogues, which were identified as Srf1 to Srf5. The crude surfactin extract (10.00 mg/mL) retained antimicrobial activity (100%) against the MDR, XDR and colistin resistant A. baumannii, P. aeruginosa, E. coli and K. pneumoniae strains. Conclusion Clinical, environmental and control strains of A. baumannii, P. aeruginosa, E. coli and K. pneumoniae exhibiting MDR and XDR profiles and colistin resistance, were susceptible to surfactin analogues, confirming that this lipopeptide shows promise for application in clinical settings.