Relevant bibliographies by topics / Spt8

Academic literature on the topic 'Spt8'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Spt8"

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Winston, Fred, Catherine Dollard, Elizabeth A. Malone, Jeffrey Clare, James G. Kapakos, Philip Farabaugh, and Patricia L. Minehart. "Three Genes Are Required for trans-Activation of Ty Transcription in Yeast." Genetics 115, no. 4 (April 1, 1987): 649–56. http://dx.doi.org/10.1093/genetics/115.4.649.

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ABSTRACT Mutations in the SPT3 gene were isolated as one class of suppressors of Ty and solo δ insertion mutations in Saccharomyces cerevisiae. Previous work has shown that null mutations in SPT3 abolish the normal Ty δ-δ transcript; instead, a transcript that initiates 800 bases farther downstream is made, suggesting that SPT3 is required for transcription initiation in δ sequences. We have selected for new spt mutations and have screened for those with the unique suppression pattern of spt3 mutations with respect to two insertion mutations. Our selection and screen has identified two additional genes, SPT7 and SPT8, that are also required for transcription initiation in δ sequences. We show that mutations in SPT7 or SPT8 result in the same alteration of Ty transcription as do mutations in SPT3. In addition, mutations in all three genes cause a sporulation defect. By assay of a Ty-lacZ fusion we have shown that spt3, spt7 and spt8 mutations reduce transcription from a δ sequence by 10-25-fold. Finally, we show that SPT3 mRNA levels are unaffected in either spt7 or spt8 mutants, suggesting that these two genes do not regulate transcription of SPT3.
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Happel, A. M., M. S. Swanson, and F. Winston. "The SNF2, SNF5 and SNF6 genes are required for Ty transcription in Saccharomyces cerevisiae." Genetics 128, no. 1 (May 1, 1991): 69–77. http://dx.doi.org/10.1093/genetics/128.1.69.

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Abstract The Saccharomyces cerevisiae SNF2, SNF5 and SNF6 genes were initially identified as genes required for expression of SUC2 and other glucose repressible genes. The Suc- defect in all three of these classes of mutants is suppressed by mutations in the SPT6 gene. Since mutations in SPT6 had also been identified as suppressors of Ty and solo delta insertion mutations at the HIS4 and LYS2 loci, we have examined Ty transcription in snf2, snf5 and snf6 mutants and have found that Ty transcription is abolished or greatly reduced. The snf2, snf5 and snf6 defect for Ty transcription, like the defect for SUC2 transcription, is suppressed by spt6 mutations. In contrast to other mutations that abolish or greatly reduce Ty transcription (in the SPT3, SPT7 and SPT8 genes), mutations in these SNF genes do not cause suppression of insertion mutations. This result suggests that the SNF2, SNF5 and SNF6 gene products act by a distinct mechanism from the SPT3, SPT7 and SPT8 gene products to promote transcription of Ty elements. This result also suggests that a reduction of Ty transcription is not always sufficient for activation of adjacent gene expression.
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Belotserkovskaya, Rimma, David E. Sterner, Min Deng, Michael H. Sayre, Paul M. Lieberman, and Shelley L. Berger. "Inhibition of TATA-Binding Protein Function by SAGA Subunits Spt3 and Spt8 at Gcn4-Activated Promoters." Molecular and Cellular Biology 20, no. 2 (January 15, 2000): 634–47. http://dx.doi.org/10.1128/mcb.20.2.634-647.2000.

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ABSTRACT SAGA is a 1.8-MDa yeast protein complex that is composed of several distinct classes of transcription-related factors, including the adaptor/acetyltransferase Gcn5, Spt proteins, and a subset of TBP-associated factors. Our results indicate that mutations that completely disrupt SAGA (deletions of SPT7 orSPT20) strongly reduce transcriptional activation at theHIS3 and TRP3 genes and that Gcn5 is required for normal HIS3 transcriptional start site selection. Surprisingly, mutations in Spt proteins involved in the SAGA-TBP interaction (Spt3 and Spt8) cause derepression of HIS3 andTRP3 transcription in the uninduced state. Consistent with this finding, wild-type SAGA inhibits TBP binding to theHIS3 promoter in vitro, while SAGA lacking Spt3 or Spt8 is not inhibitory. We detected two distinct forms of SAGA in cell extracts and, strikingly, one lacks Spt8. Conditions that induceHIS3 and TRP3 transcription result in an altered balance between these complexes strongly in favor of the form without Spt8. These results suggest that the composition of SAGA may be dynamic in vivo and may be regulated through dissociable inhibitory subunits.
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Eisenmann, D. M., C. Chapon, S. M. Roberts, C. Dollard, and F. Winston. "The Saccharomyces cerevisiae SPT8 gene encodes a very acidic protein that is functionally related to SPT3 and TATA-binding protein." Genetics 137, no. 3 (July 1, 1994): 647–57. http://dx.doi.org/10.1093/genetics/137.3.647.

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Abstract Mutations in the Saccharomyces cerevisiae SPT8 gene were previously isolated as suppressors of retrotransposon insertion mutations in the 5' regions of the HIS4 and LYS2 genes. Mutations in SPT8 confer phenotypes similar to those caused by particular mutations in SPT15, which encodes the TATA-binding protein (TBP). These phenotypes are also similar to those caused by mutations in the SPT3 gene, which encodes a protein that directly interacts with TBP. We have now cloned and sequenced the SPT8 gene and have shown that it encodes a predicted protein of 602 amino acids with an extremely acidic amino terminus. In addition, the predicted SPT8 amino acid sequence contains one copy of a sequence motif found in multiple copies in a number of other eukaryotic proteins, including the beta subunit of heterotrimeric G proteins. To investigate further the relationship between SPT8, SPT3 and TBP, we have analyzed the effect of an spt8 null mutation in combination with different spt3 and spt15 mutations. This genetic analysis has shown that an spt8 deletion mutation is suppressed by particular spt3 alleles. Taken together with previous results, these data suggest that the SPT8 protein is required, directly or indirectly, for TBP function at particular promoters and that the role of SPT8 may be to promote a functional interaction between SPT3 and TBP.
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Roberts, S. M., and F. Winston. "SPT20/ADA5 encodes a novel protein functionally related to the TATA-binding protein and important for transcription in Saccharomyces cerevisiae." Molecular and Cellular Biology 16, no. 6 (June 1996): 3206–13. http://dx.doi.org/10.1128/mcb.16.6.3206.

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Mutations selected as suppressors of Ty and solo delta insertion mutations is Saccharomyces cerevisiae have identified a number of genes important for transcription initiation. One of these gens, SPT15, encodes the TATA-binding protein, and three others, SPT3, SPT7, and SPT8, encode proteins functionally related to the TATA-binding protein. To identify additional related functions, we have selected for new spt mutations. This work has identified one new gene, SPT20. Null mutations in SPT20 cause poor growth and a set of severe transcriptional defects very similar to those caused by null mutations in SPT3, SPT7, and SPT8 and also very similar to those caused by certain missense mutations in SPT15. Consistent with its having an important function in transcription in vivo, SPT20 was also recently identified as ADA5 and has been shown to be important for transcriptional activation (G.A. Marcus, J. Horiuchi, N. Silverman, and L. Guarente, Mol. Cell. Biol. 16:3197-3205, 1996.
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Happel, A. M., and F. Winston. "A mutant tRNA affects delta-mediated transcription in Saccharomyces cerevisiae." Genetics 132, no. 2 (October 1, 1992): 361–74. http://dx.doi.org/10.1093/genetics/132.2.361.

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Abstract Mutations in the SPT3, SPT7, SPT8 and SPT15 genes define one class of trans-acting mutations that are strong suppressors of insertion mutations caused by Ty elements or by the Ty long terminal repeat sequence, delta. These SPT genes are required for normal transcription of Ty elements, and their gene products are believed to be involved in initiation of Ty transcription from delta sequences. We have isolated and analyzed extragenic suppressors of spt3 mutations. These new mutations, named rsp, partially suppress the requirement for SPT3, SPT7, SPT8 and SPT15 functions. In addition, rsp mutations cause changes in transcription of some delta insertions in an SPT+ genetic background. Interactions between mutations in the four identified RSP genes show a number of interesting genetic properties, including the failure of unlinked rsp mutations to complement for recessive phenotypes. Cloning and sequencing of one rsp mutant gene, rsp4-27, showed that it encodes a frameshift suppressor glycine tRNA. Our results indicate that the other three RSP genes also encode frameshift suppressor glycine tRNAs. In addition, other types of frameshift suppressor glycine tRNAs can confer some Rsp- phenotypes.
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Sterner, David E., Patrick A. Grant, Shannon M. Roberts, Laura J. Duggan, Rimma Belotserkovskaya, Lisa A. Pacella, Fred Winston, Jerry L. Workman, and Shelley L. Berger. "Functional Organization of the Yeast SAGA Complex: Distinct Components Involved in Structural Integrity, Nucleosome Acetylation, and TATA-Binding Protein Interaction." Molecular and Cellular Biology 19, no. 1 (January 1, 1999): 86–98. http://dx.doi.org/10.1128/mcb.19.1.86.

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ABSTRACT SAGA, a recently described protein complex in Saccharomyces cerevisiae, is important for transcription in vivo and possesses histone acetylation function. Here we report both biochemical and genetic analyses of members of three classes of transcription regulatory factors contained within the SAGA complex. We demonstrate a correlation between the phenotypic severity of SAGA mutants and SAGA structural integrity. Specifically, null mutations in the Gcn5/Ada2/Ada3 or Spt3/Spt8 classes cause moderate phenotypes and subtle structural alterations, while mutations in a third subgroup, Spt7/Spt20, as well as Ada1, disrupt the complex and cause severe phenotypes. Interestingly, double mutants (gcn5Δ spt3Δand gcn5Δ spt8Δ) causing loss of a member of each of the moderate classes have severe phenotypes, similar tospt7Δ, spt20Δ, or ada1Δmutants. In addition, we have investigated biochemical functions suggested by the moderate phenotypic classes and find that first, normal nucleosomal acetylation by SAGA requires a specific domain of Gcn5, termed the bromodomain. Deletion of this domain also causes specific transcriptional defects at the HIS3 promoter in vivo. Second, SAGA interacts with TBP, the TATA-binding protein, and this interaction requires Spt8 in vitro. Overall, our data demonstrate that SAGA harbors multiple, distinct transcription-related functions, including direct TBP interaction and nucleosomal histone acetylation. Loss of either of these causes slight impairment in vivo, but loss of both is highly detrimental to growth and transcription.
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van Oevelen, Chris J. C., Hetty A. A. M. van Teeffelen, and H. T. Marc Timmers. "Differential Requirement of SAGA Subunits for Mot1p and Taf1p Recruitment in Gene Activation." Molecular and Cellular Biology 25, no. 12 (June 15, 2005): 4863–72. http://dx.doi.org/10.1128/mcb.25.12.4863-4872.2005.

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ABSTRACT Transcription activation in yeast (Saccharomyces cerevisiae) involves ordered recruitment of transcription factor complexes, such as TFIID, SAGA, and Mot1p. Previously, we showed that both Mot1p and Taf1p are recruited to the HXT2 and HXT4 genes, which encode hexose transporter proteins. Here, we show that SAGA also binds to the HXT2 and HXT4 promoters and plays a pivotal role in the recruitment of Mot1p and Taf1p. The deletion of either SPT3 or SPT8 reduces Mot1p binding to HXT2 and HXT4. Surprisingly, the deletion of GCN5 reduces Taf1p binding to both promoters. When GCN5 is deleted in spt3Δ or spt8Δ strains, neither Mot1p nor Taf1p binds, and this results in a diminished recruitment of TATA binding protein and polymerase II to the HXT4 but not the HXT2 promoter. This is reflected by the SAGA-dependent expression of HXT4. In contrast, SAGA-independent induction of HXT2 suggests a functional redundancy with other factors. A functional interplay of different SAGA subunits with Mot1p and Taf1p was supported by phenotypic analysis of MOT1 SAGA or TAF1/SAGA double mutant strains, which revealed novel genetic interactions between MOT1 and SPT8 and between TAF1 and GCN5. In conclusion, our data demonstrate functional links between SAGA, Mot1p, and TFIID in HXT gene regulation.
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Swanson, M. S., and F. Winston. "SPT4, SPT5 and SPT6 interactions: effects on transcription and viability in Saccharomyces cerevisiae." Genetics 132, no. 2 (October 1, 1992): 325–36. http://dx.doi.org/10.1093/genetics/132.2.325.

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Abstract The SPT4, SPT5 and SPT6 genes of Saccharomyces cerevisiae were identified originally by mutations that suppress delta insertion mutations at HIS4 and LYS2. Subsequent analysis has demonstrated that spt4, spt5 and spt6 mutations confer similar pleiotropic phenotypes. They suppress delta insertion mutations by altering transcription and are believed to be required for normal transcription of several other loci. We have now analyzed interactions between SPT4, SPT5 and SPT6. First, the combination of mutations in any two of these three genes causes lethality in haploids. Second, some recessive mutations in different members of this set fail to complement each other. Third, mutations in all three genes alter transcription in similar ways. Finally, the results of coimmunoprecipitation experiments demonstrate that at least the SPT5 and SPT6 proteins interact physically. Taken together, these genetic and biochemical results indicate that SPT4, SPT5 and SPT6 function together in a transcriptional process that is essential for viability in yeast.
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Shao, Wei, Zhan Ding, Zeng-Zhang Zheng, Ji-Jia Shen, Yu-Xian Shen, Jia Pu, Yu-Jie Fan, Charles C. Query, and Yong-Zhen Xu. "Prp5−Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription." Nucleic Acids Research 48, no. 11 (May 13, 2020): 5799–813. http://dx.doi.org/10.1093/nar/gkaa311.

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Abstract Transcription and pre-mRNA splicing are coupled to promote gene expression and regulation. However, mechanisms by which transcription and splicing influence each other are still under investigation. The ATPase Prp5p is required for pre-spliceosome assembly and splicing proofreading at the branch-point region. From an open UV mutagenesis screen for genetic suppressors of prp5 defects and subsequent targeted testing, we identify components of the TBP-binding module of the Spt–Ada–Gcn5 Acetyltransferase (SAGA) complex, Spt8p and Spt3p. Spt8Δ and spt3Δ rescue the cold-sensitivity of prp5-GAR allele, and prp5 mutants restore growth of spt8Δ and spt3Δ strains on 6-azauracil. By chromatin immunoprecipitation (ChIP), we find that prp5 alleles decrease recruitment of RNA polymerase II (Pol II) to an intron-containing gene, which is rescued by spt8Δ. Further ChIP-seq reveals that global effects on Pol II-binding are mutually rescued by prp5-GAR and spt8Δ. Inhibited splicing caused by prp5-GAR is also restored by spt8Δ. In vitro assays indicate that Prp5p directly interacts with Spt8p, but not Spt3p. We demonstrate that Prp5p's splicing proofreading is modulated by Spt8p and Spt3p. Therefore, this study reveals that interactions between the TBP-binding module of SAGA and the spliceosomal ATPase Prp5p mediate a balance between transcription initiation/elongation and pre-spliceosome assembly.
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Dissertations / Theses on the topic "Spt8"

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Bhat, Abdul Wajid. "Regulation of transcription elongation factors SPT2 and SPT6 by casein kinase II." Thesis, Université Laval, 2013. http://www.theses.ulaval.ca/2013/29184/29184.pdf.

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Comme pour tous les autres processus en lien avec l’ADN, la structure de la chromatine lors de la transcription est dans un état de perpétuel changement. Ainsi, elle est ouverte pour permettre l’accès à l’ADN, pour ensuite se replier correctement. La dynamique de la structure chromatinienne est régulée finement par de multiples mécanismes qui agissent ensemble afin de rendre le processus hautement efficace. Ces mécanismes comprennent les modifications post-traductionelles des histones, le remodelage de la chromatine par les remodeleurs ATP-dépendants, l’incorporation des variants d’histones et l’assemblage/désassemblage des nucléosomes par les chaperons d’histones. En plus de ces activités, il y a un certain nombre de composantes non-reliées aux histones qui sont directement impliquées dans les modulations de la conformation de la chromatine associées à la transcription. Chez la levure, un de ces facteurs est la protéine HMG-like Spt2p, démontrée précédemment comme étant directement impliquée dans le réassemblage des nucléosomes dans le sillon de l’ARN polymérase II en déplacement le long du segment d’ADN transcrit. Dans la présente étude, nous démontrons que Spt2p est phosphorylée directement par la caséine kinase II (CKII) et que cette modification inhibe sa liaison à la chromatine. Nos résultats indiquent que la CKII altère l’interaction de Spt2p avec le chaperon d’histone Spt6p. Nous avons aussi trouvé que la phosphorylation directe de Spt6p par la CKII stimule l’association de ce facteur avec un autre partenaire, Iws1p. Cette association est absolument nécessaire pour le repliement correct des nucléosomes durant l’élongation. De plus, cette régulation positive du complexe Spt6p/Iws1p par la CKII module directement l’association de ce complexe avec la méthyltransférase de H3K36, Set2p. Finalement, nous avons montré que la phosphorylation de Spt6p par la CKII est essentielle à l’inhibition des promoteurs cryptiques et des erreurs de transcription. Dans l’ensemble, nos résultats suggèrent un nouveau mécanisme par lequel la CKII contrôle le repliement correct de la structure de la chromatine dans les régions codantes en modulant les interactions du chaperon d’histone essentiel Spt6p avec ses partenaires Spt2p, Iws1p et Set2p.
Like any other DNA-related process, chromatin structure is in a state of constant flux during transcription, unfolded to get access to DNA and refolded back properly. The dynamics of chromatin structure are tightly regulated and multiple mechanisms act together to make the process highly efficient. These include modifications of histones, chromatin remodeling by ATP-dependent remodeling factors, incorporation of histone variants and nucleosome disassembly and reassembly by histone chaperones. In addition to these activities, there are a number of non-histone chromatin components that are directly involved in the modulation of chromatin associated with transcription. In yeast, one of these factors is the HMG-like protein Spt2p previously shown to participate directly in the process of nucleosome reassembly in the wake of RNA polymerase II movement along transcribed DNA. In this work, we show that Spt2p is directly phosphorylated by the casein kinase II (CKII) and we demonstrate that this modification inhibits its association with chromatin. Our findings indicate that CKII disrupts the interaction of Spt2p with the histone chaperone Spt6p. Interestingly, we also found that direct phosphorylation of Spt6p by CKII stimulates the association of this factor with another partner, Iws1p. This association is absolutely required for the refolding of nucleosomes during elongation. Furthermore, this positive regulation of the Spt6p/Iws1p complex by CKII modulates directly the association of this complex with the H3K36 methyltransferase Set2p. Finally, we show that phosphorylation of Spt6p by CKII is essential to the inhibition of cryptic promoters and spurious transcription. Taken together, our results suggest a new mechanism whereby CKII directs chromatin structure refolding in coding regions by modulating the interaction of the essential histone chaperone Spt6p with its partners Spt2p, Iws1p and Set2p.
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Dürr, Julius [Verfasser], and Klaus [Akademischer Betreuer] Grasser. "The role of the transcription elongation factor SPT4-SPT5 in plant growth and development / Julius Dürr. Betreuer: Klaus Grasser." Regensburg : Universitätsbibliothek Regensburg, 2015. http://d-nb.info/1065445318/34.

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Vojnić, Erika. "NMR solution structure of the Set2 SRI domain and preparation of RNA polymerase II complexes with the elongation factor Spt4-Spt5." [S.l.] : [s.n.], 2006. http://edoc.ub.uni-muenchen.de/archive/00006976.

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Vojnic, Erika. "NMR solution structure of the Set2 SRI domain and preparation of RNA polymerase II complexes with the elongation factor Spt4-Spt5." Diss., lmu, 2006. http://nbn-resolving.de/urn:nbn:de:bvb:19-69769.

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Burckin, Todd A. "Probing the integration of steps in the gene expression pathway through analysis of the SPT4-SPT5 transcription elongation complex in Saccharomyces cervisiae /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2004. http://uclibs.org/PID/11984.

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Howarth, Clayton. "Motorinformationens roll i SPT-effekten." Thesis, University of Skövde, School of Humanities and Informatics, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-940.

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Det har visat sig vara bättre att öva in listor med handlingsfraser genom att utföra dem (SPT, subject-performed tasks) än genom att bara läsa dem (VT, verbal tasks). Vid ett återerinringstest visar sig SPT-effekten då försöksdeltagare med SPTs har ett mycket bättre minne av materialet än försöksdeltagare med VTs. En förklaring till fenomenet är att utförandet av handlingsfraserna förser deltagarna med motorinformation. I den här undersökningen testas motorinformationens roll i SPT-effekten på ett sätt som skiljer sig från traditionell SPT-forskning. Försöksdeltagare fick antingen cykla eller använda en joystick för att navigera genom en virtuell värld där ord fanns utplacerade. Minnet för orden testades sedan i ett efterföljande minnestest. Det visade sig att joystickgruppen kunde återerinra sig fler ord än cykelgruppen. Effekten var oväntad och misstänks bero på bättre koncentrationsmöjligheter för joystickgruppen.

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Kasberg, Abigail D. "Sp8 Function During Craniofacial Development." University of Cincinnati / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1396452767.

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Belincanta, Antonio. "Avaliação de fatores intervenientes no Índice de Resistência à Penetração do SPT." Universidade de São Paulo, 1998. http://www.teses.usp.br/teses/disponiveis/18/18132/tde-12062018-162336/.

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São salientados neste trabalho de pesquisa alguns pontos importantes da origem, evolução e normatização do ensaio SPT (\"Standard Penetration Test\") no Brasil e no exterior, bem como analisadas variantes, em uso, do método de ensaio proposto pela ABNT (Associação Brasileira de Normas Técnicas), tais como acionamento com cabo de aço e avanço da perfuração feita pela cravação do próprio amostrador, apresentando-se uma avaliação preliminar da influência nos valores dos índices de resistência à penetração N do SPT. Variáveis tidas como de relevância também serão avaliadas, com a quantificação da influência delas, de forma preliminar. Pode-se citar, entre estas variáveis, o tipo do martelo, o dispositivo para queda livre, o uso de roldana móvel, tamanho da cabeça de bater, estado de conservação da composição das hastes e o uso ou não do coxim de madeira. Para a análise e avaliação tanto dos métodos variantes quanto das variáveis intervenientes foram coletados dados em sondagens/ensaios realizados, em quatro cidades de três Estados brasileiros, por seis empresas selecionadas e escolhidas em conformidade aos interesses específicos dos trabalhos. Por fim, são feitas sugestões para continuidade dos trabalhos de pesquisa, visando a melhoria deste tipo de sondagem/ensaio, quanto ao entendimento de suas variáveis, evolução, normatização e, consequentemente, sua padronização.
This research work presents some important aspects of the origin, evolution and standardization of the Standard Penetration Test (SPT), both in Brazil and abroad. It will also introduces the currently used variations of the proposed ABNT (Brazilian Association of Technical Standard) test method, which encompass the use of steel rope and drilling advance by sampler driving, and a preliminary evaluation on N values. Finally, it also analyses the influence on N results, of variables such as type of hammer, free weight fall system, use of mobile sheave wheel, size of anvil, state of conservation of sampling rods and use or not of a hard wood cushion. Analysis and evaluation were performed using selected data obtained from six different companies which carried out tests in four cities at three different Brazilian States. Finally suggestions are made for the continuity of research works on this subject aiming SPT test improvement by the understanding of the variables which affect N values which will contribute to its standardization.
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Bayliss, M. B., J. Ruel, C. W. Stubbs, S. W. Allen, D. E. Applegate, M. L. N. Ashby, M. Bautz, et al. "SPT-GMOS: A GEMINI/GMOS-SOUTH SPECTROSCOPIC SURVEY OF GALAXY CLUSTERS IN THE SPT-SZ SURVEY." IOP PUBLISHING LTD, 2016. http://hdl.handle.net/10150/622337.

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We present the results of SPT-GMOS, a spectroscopic survey with the Gemini Multi-Object Spectrograph (GMOS) on Gemini South. The targets of SPT-GMOS are galaxy clusters identified in the SPT-SZ survey, a millimeter-wave survey of 2500 deg(2) of the southern sky using the South Pole Telescope (SPT). Multi-object spectroscopic observations of 62 SPT-selected galaxy clusters were performed between 2011 January and 2015 December, yielding spectra with radial velocity measurements for 2595 sources. We identify 2243 of these sources as galaxies, and 352 as stars. Of the galaxies, we identify 1579 as members of SPT-SZ galaxy clusters. The primary goal of these observations was to obtain spectra of cluster member galaxies to estimate cluster redshifts and velocity dispersions. We describe the full spectroscopic data set and resulting data products, including galaxy redshifts, cluster redshifts, and velocity dispersions, and measurements of several well-known spectral indices for each galaxy: the equivalent width, W, of [O II] lambda lambda 3727, 3729 and H-delta, and the 4000 angstrom break strength, D4000. We use the spectral indices to classify galaxies by spectral type (i.e., passive, post-starburst, star-forming), and we match the spectra against photometric catalogs to characterize spectroscopically observed cluster members as a function of brightness (relative to m*). Finally, we report several new measurements of redshifts for ten bright, strongly lensed background galaxies in the cores of eight galaxy clusters. Combining the SPT-GMOS data set with previous spectroscopic follow-up of SPT-SZ galaxy clusters results in spectroscopic measurements for >100 clusters, or similar to 20% of the full SPT-SZ sample.
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Loeliger, Erin Michelle. "Structure-Function Analysis of the Conserved Histone Chaperone Spt6." Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11369.

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Chromatin structure is crucial to regulate access to the genome for processes such as a transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin structure, is conserved throughout eukaryotes. Spt6 has been shown to function in many aspects of gene expression, including nucleosome assembly, transcription initiation and elongation, and mRNA processing and export. In addition, Spt6 has several conserved domains; however, little is known about their functions. I have performed a structure-function analysis of Spt6 using three separate approaches. First, I employed a random insertion mutagenesis that has identified sixty-seven mutants. While these mutants did not provide information regarding known domains, some have phenotypes that may prove useful for future study. Second, in a collaborative project with Romier lab, I studied the functional roles of the Spt6 SH2 domains. I have shown that deletion of the region of Spt6 encoding the SH2 domains causes severe mutant phenotypes without affecting Spt6 protein levels, demonstrating the importance of the SH2 domains of Spt6. Third, in an additional collaboration with the Romier lab, I showed that mutations that alter the region of Spt6 that interacts with the conserved transcription factor Spn1 impair Spt6 functions in vivo. Overall, this multi-pronged structure-function analysis of Spt6 has provided new insights into the tandem SH2 domains of Spt6, the Spt6-Spn1 interaction, and the uses and limitations of insertion mutagenesis. In addition, I have attempted to explore a possible role for Spt6 in transcription-associated mutagenesis. After employing several types of in vivo assays, I conclude that a possible role for Spt6 in transcription-associated mutagenesis is uncertain, as the results (with respect to a role for Spt6) reproducibly vary depending on the assay used. Thus, understanding this aspect of Spt6 biology awaits better assays and understanding of transcription-associated mutagenesis. Overall, the work in this dissertation will serve to further elucidate the mechanisms of Spt6 in chromatin regulation, transcription, and DNA damage repair.
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Books on the topic "Spt8"

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Glazyrin, V. V. Uchashchiĭsi︠a︡ SPTU: Prava, obi︠a︡zannosti. Moskva: I︠U︡rid. lit-ra, 1987.

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Glazyrin, V. V. Uchashchiisya SPTU: prava, obyazannosti. Moskva: Yurid. lit-ra, 1987.

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Manzella, David. High voltage SPT performance. [Cleveland, Ohio]: National Aeronautics and Space Administration, Glenn Research Center, 2001.

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Kınıklı, Nuriye Esra. Analiz yöntemlerine genel bakiş ve analizde SPT yöntemi. Eskişehir: Anadolu Üniversitesi yayınları, 2008.

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(Publisher), Graha Ilmu. SPT elektronik, PPh pekerja ditanggung pemerintah dan bebas fiskal. Yogyakarta: Graha Ilmu, 2009.

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obrazovanieto, Bulgaria Ministerstvo na naukata i. Sbornik uchebna dokumentat︠s︡ii︠a︡ za SPTU po selsko stopanstvo: Profesii︠a︡ N. 030306 - zemedelet︠s︡. Sofii︠a︡: Ministerstvo na naukata i obrazovabieto, 1994.

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Winter, Charles J. Investigation of standard penetration torque testing (SPT-T) to predict pile performance. Madison, WI: Wisconsin Highway Research Program, University of Wisconsin-Madison, 2005.

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Nachev, Nachko. Izsledvanii͡a︡ za opredeli͡a︡ne tematikata po metodika na obuchenieto po tekhnicheskite dist͡s︡iplini v tekhnikumite, SPTU i ESPU. Gabrovo: [s.n.], 1986.

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Sai er hao SPT xian feng dui: Ji po! ling yu xian jing. Nanjing: Jiang su shao nian er tong chu ban she, 2012.

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Chenxi, Xiao. Sai er hao SPT xian feng dui: Ji zhan long mian sha mo. Nanjing: Jiang su shao nian er tong chu ban she, 2011.

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Book chapters on the topic "Spt8"

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Mnad, Mouna Tka, Christophe Deleuze, and Ioannis Parissis. "Synchronous Programs Testing Language (SPTL)." In Computational Science and Its Applications – ICCSA 2014, 683–95. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-09144-0_47.

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Radzevich, Stephen P. "SPTS – Split Power Transmission Systems." In Theory of Gearing, 809–38. 3rd ed. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003311744-33.

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Singh, Rishabh, and Armando Solar-Lezama. "SPT: Storyboard Programming Tool." In Computer Aided Verification, 738–43. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31424-7_58.

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Lutenegger, Alan J. "Standard Penetration Test (SPT)." In In Situ Testing Methods in Geotechnical Engineering, 13–72. First edition. | Boca Raton, FL : CRC Press, 2021.: CRC Press, 2021. http://dx.doi.org/10.1201/9781003002017-2.

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Pettersson, Holger, and Hans Ringertz. "SP8 Thoracic kyphosis/age [radiography]." In Measurements in Pediatric Radiology, 34–35. London: Springer London, 1991. http://dx.doi.org/10.1007/978-1-4471-1844-2_17.

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Dorsch, Jörg, Anders Ek, and Reinhard Gotzhein. "SPT – The SDL Pattern Tool." In System Analysis and Modeling, 50–64. Berlin, Heidelberg: Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/978-3-540-31810-1_4.

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Morgano, C. M., and R. Liang. "Energy transfer in SPT – Rod length effect." In Application of Stress-Wave Theory to Piles, 121–27. London: Routledge, 2022. http://dx.doi.org/10.1201/9781315137544-18.

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Sermalai, Selvam, Manoj Mukundan, and Swathi Alagirisamy. "Standard Penetration Test (SPT) Pitfalls and Improvements." In Lecture Notes in Civil Engineering, 363–75. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-3383-6_33.

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Zachariah, Jithin P., and Ravi S. Jakka. "Liquefaction Potential of Ash Pond Using SPT." In Lecture Notes in Civil Engineering, 27–34. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-9976-7_3.

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Monnet, Jacques. "Penetrometer Test (CPT, CPTU, SPT, DCPT) and Variants." In In Situ Tests in Geotechnical Engineering, 113–56. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119145592.ch7.

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Conference papers on the topic "Spt8"

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Matloff, Gregory L. "The Holographic Solar Photon Thruster (SPT) : A Low-Earth Orbit Solar Sail." In ASME 2003 International Solar Energy Conference. ASMEDC, 2003. http://dx.doi.org/10.1115/isec2003-44059.

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Atmospheric drag limits most solar sails to altitudes>1000 km. A two-sail variant, the Solar-Photon Thruster (SPT) , could be used in Low-Earth Orbit (LEO). An SPT has a fixed-orientation collector sail that focuses light against a smaller, adjustable thruster sail. Maintaining the collector surface parallel to the Earth minimizes SPT drag in LEO. To minimize solar-radiation back pressure towards Earth, the upper collector surface is non-reflective. The reflective lower collector surface directs light reflected and reradiated from the Earth against the thruster. Thruster orientation is adjusted in LEO to increase the orbital energy by the net radiation-pressure. Experiments reveal that holograms are tolerant to solar-wind radiation. SPTs with white-light holographic thrusters are useful in LEO because small thruster rotations produce greatly altered reflectivity. It may be possible to holographically combine SPT collector and thruster.
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Bahat, Anat, and Rivka Dikstein. "I14 Development of drugs against SPT4/SPT5 for the treatment of Huntington’s disease." In EHDN 2022 Plenary Meeting, Bologna, Italy, Abstracts. BMJ Publishing Group Ltd, 2022. http://dx.doi.org/10.1136/jnnp-2022-ehdn.240.

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Li, R., T. H. Hyde, W. Sun, and B. Dogan. "Modelling and Data Interpretation of Small Punch Creep Testing." In ASME 2011 Pressure Vessels and Piping Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/pvp2011-57204.

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The small punch testing (SPT) technique has been proposed for use in determining the creep properties of materials for which only a very small volume of material is available. A draft code of practice on SPT has been produced. However it is not, as yet, generally accepted that the data obtained from small punch tests can be directly related to those which would be obtained from conventional uniaxial creep tests. For this reason, the development of techniques suitable for the interpretation of SPT data has become very important. In this paper, a set of uniaxial creep test data has been characterised in such a way as to gain an improved understanding of the correlation between the data from small punch tests and corresponding uniaxial creep tests. Finite element (FE) analyses of small punch creep tests, using a damage mechanics based creep model, have been performed. The effect of large deformation on the determination of material properties for a creep damage model, has been investigated to take into account the large deformation nature of small punch tests. An equivalent stress, σeq, proposed by the draft code, was used to relate the SPT results to the corresponding uniaxial creep test results. A preliminary assessment of the use of small punch test results, in determining creep properties, has been presented, which includes comparisons of the failure life and equivalent minimum strain rate results obtained from SPTs with the corresponding uniaxial creep test data. Future work related to the interpretation of SPT is briefly addressed.
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Jia, Ning, Chun Yang, Yu He, and Xu Cheng. "SPTU." In International Conference. New York, New York, USA: ACM Press, 2014. http://dx.doi.org/10.1145/2611354.2611368.

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Lumb, Christopher R., and Richard Golding. "D-SPTF." In the 11th international conference. New York, New York, USA: ACM Press, 2004. http://dx.doi.org/10.1145/1024393.1024399.

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Lirk, P. "SP38 THA." In ESRA Abstracts, 39th Annual ESRA Congress, 22–25 June 2022. BMJ Publishing Group Ltd, 2022. http://dx.doi.org/10.1136/rapm-2022-esra.43.

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Niu, Changan, Chuanyi Li, Vincent Ng, Jidong Ge, Liguo Huang, and Bin Luo. "SPT-code." In ICSE '22: 44th International Conference on Software Engineering. New York, NY, USA: ACM, 2022. http://dx.doi.org/10.1145/3510003.3510096.

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Dogan, Bilal, and Thomas Hyde. "Industrial Application of Small Punch Testing for In-Service Component Condition Assessment: An Overview." In ASME 2012 Pressure Vessels and Piping Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/pvp2012-78691.

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The Sampling and Small Punch testing (SPT) is a powerful technique based on tests using miniaturized specimens machined out small sampled material of components in service. At present, it is the only existing method capable of providing experimental characterization of service exposed materials of components and materials of new built plants. Small sampling is non-invasive and SPT provides direct measured material properties. It provides a significant technology capability that facilitates assessing power plant operating equipment for structural integrity and operational condition. The new method provides utility members an attractive option to interrogate equipment for making run/inspect/repair/replace decisions. The SPT technique supported by assessment software, NDE and Metallography, used to define guidelines for components life assessment cross the power generation and petro-chemical sectors, serving both utilities, and constructors. It addresses the industrial need for personalized material and welds data required for a) lifing of plant; consumed life and residual life of components, b) convenience of repairing, replacing, life of the new welds on old components, c) cost of component deterioration, cost of normal service, d) characterizations and qualifications of blade repairs, of coating materials-methods. The first international SPT workshop was organized in June 27–28, 2011 in Nottingham, UK in order to discuss the state-of-the art SPT Creep and Fracture, and the draft CEN Cope of Practice (COP). The International SPT Experts Group serves as international forum for discussion and collaboration of industrial application of SPT methodology for in-service component life assessment. It is noted the draft CEN COP needs to be revised. Presently, European, Japanese and Indian national SPT project groups are running SPT tests and working on analysis programs. The present paper reports on a) the use of SPT in materials and component characterization, and b) drafted technical program by the international experts group to harmonize international efforts on SPT testing and analysis for efficiency and cost effectiveness. The draft program to bring the SPT methodology to standardization and develop an engineering component condition assessment tool for industrial application.
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Fomina, Yuliya I. "Specificities of Handling Conflict Situations by Young People with Different Types of Ethnic Identity." In Wellbeing and Security in the Face of Social Transformations. Liberal Arts University – University for Humanities, 2019. http://dx.doi.org/10.35853/lau.ws.2019.sp08.

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Zotova, Olga Yu, and Olga Yu Fleitlikh. "A Non-Binary Approach to Gender in the Context of Personality Psychological Security in a Transitive Society." In Wellbeing and Security in the Face of Social Transformations. Liberal Arts University – University for Humanities, 2019. http://dx.doi.org/10.35853/lau.ws.2019.sp18.

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Reports on the topic "Spt8"

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Engelman, S. F., and John M. Fife. Hemispherical Measurements of the SPT - 140 Plume. Fort Belvoir, VA: Defense Technical Information Center, June 2002. http://dx.doi.org/10.21236/ada410330.

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Stuckey, P., C. Clauss, M. Day, V. Murashko, and N. Maslennikov. SPT-140 High Performance Hall System (HPHS) Development. Fort Belvoir, VA: Defense Technical Information Center, July 1998. http://dx.doi.org/10.21236/ada410741.

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Prezzi, Monica, Seth Scheilz, Rodrigo Salgado, and Nayyar Zia Siddiki. Development of SPT-Torque Test Correlations for Glacial Till. Purdue University, June 2017. http://dx.doi.org/10.5703/1288284315499.

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Yokel, Felix Y. Effect of blow count on energy transfer in SPT. Gaithersburg, MD: National Bureau of Standards, 1988. http://dx.doi.org/10.6028/nbs.ir.88-3765.

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Sheffield, Kimberly. Interplay of Transcription Factor E and Spt4/5 During Transcription Initiation in Pyrococcus furiosus. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.6328.

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Fife, J. M., W. A. Hargus Jr, D. A. Jaworske, C. Sarmiento, L. Mason, R. Jankovsky, J. S. Snyder, S. Malone, J. Haas, and A. Gallimore. Spacecraft Interaction Test Results of the High Performance Hall System SPT-140 (Postprint). Fort Belvoir, VA: Defense Technical Information Center, July 2000. http://dx.doi.org/10.21236/ada471175.

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Hargus, W., Jankovsky Jr., Mason R., Snyder L., Malone J., and S. Status of US Testing of the High Performance Hall System SPT-140 Hall Thruster. Fort Belvoir, VA: Defense Technical Information Center, January 2000. http://dx.doi.org/10.21236/ada410594.

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Nakles, Michael R., Jr Hargus, Delgado William A., Corey Jorge J., and Ronald L. A Performance Comparison of Xenon and Krypton Propellant on an SPT-100 Hall Thruster (Preprint). Fort Belvoir, VA: Defense Technical Information Center, August 2011. http://dx.doi.org/10.21236/ada549666.

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Weinschenk, Craig, Daniel Madrzykowski, and Paul Courtney. Impact of Flashover Fire Conditions on Exposed Energized Electrical Cords and Cables. UL Firefighter Safety Research Institute, October 2019. http://dx.doi.org/10.54206/102376/hdmn5904.

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A set of experiments was conducted to expose different types of energized electrical cords for lamps, office equipment, and appliances to a developing room fire exposure. All of the cords were positioned on the floor and arranged in a manner to receive a similar thermal exposure. Six types of cords commonly used as power supply cords, extension cords, and as part of residential electrical wiring systems were chosen for the experiments. The non-metallic sheathed cables (NMB) typically found in residential electrical branch wiring were included to provide a link to previous research. The basic test design was to expose the six different types of cords, on the floor of a compartment to a growing fire to determine the conditions under which the cord would trip the circuit breaker and/or undergo an arc fault. All of the cords would be energized and installed on a non-combustible surface. Six cord types (18-2 SPT1, 16-3 SJTW, 12-2 NM-B, 12-3 NM-B, 18-3 SVT, 18-2 NISPT-2) and three types of circuit protection (Molded case circuit breaker (MCCB), combination Arc-fault circuit interrupter (AFCI), Ground-fault circuit interrupter (GFCI)) were exposed to six room-scale fires. The circuit protection was remote from the thermal exposure. The six room fires consisted of three replicate fires with two sofas as the main fuel source, two replicate fires with one sofa as the main fuel source and one fire with two sofas and MDF paneling on three walls in the room. Each fuel package was sufficient to support flashover conditions in the room and as a result, the impact on the cords and circuit protection was not significantly different. The average peak heat release rate of the sofa fueled compartment fires with gypsum board ceiling and walls was 6.8 MW. The addition of vinyl covered MDF wall paneling on three of the compartment walls increased the peak heat release rate to 12 MW, although most of the increased energy release occurred outside of the compartment opening. In each experiment during post flashover exposure, the insulation on the cords ignited and burned through, exposing bare conductor. During this period the circuits faulted. The circuit protection devices are not designed to provide thermal protection, and, thus, were installed remote from the fire. The devices operated as designed in all experiments. All of the circuit faults resulted in either a magnetic trip of the conventional circuit breaker or a ground-fault trip in the GFCI or AFCI capable circuit protection devices. Though not required by UL 1699, Standard for Safety for Arc-Fault Circuit-Interrupters as the solution for detection methodology, the AFCIs used had differential current detection. Examination of signal data showed that the only cord types that tripped with a fault to ground were the insulated conductors in non-metallic sheathed cables (12-2 NM-B and 12-3 NM-B). This was expected due to the bare grounding conductor present. Assessments of both the thermal exposure and physical damage to the cords did not reveal any correlation between the thermal exposure, cord damage, and trip type.
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