Dissertations / Theses on the topic 'Spot blotch'

Spot form net blotch (SFNB) caused by Pyrenophora teres f. maculata is a major foliar disease of barley (Hordeum vulgare L.) worldwide. SFNB epidemics have recently been observed in major barley producing countries, suggesting that the local barley cultivars are not resistant and that virulence of the pathogen populations may have changed. Here we attempt to identify sources of resistance effective against diverse isolates of the SFNB pathogen. A total of 2062 world barley core collection accessions were phenotyped using isolates of this pathogen collected in the United States (FGO), Australia (SG1), New Zealand (NZKF2), and Denmark (DEN 2.6). Isolate-specific susceptibility was identified in several of the barley accessions tested, indicating variability in both pathogen virulence and host resistance/susceptibility. Collectively, only 15 barley accessions were resistant across all isolates tested. Future research will involve the characterization of host resistance, pathogen virulence, and the host-pathogen interaction associated with SFNB of barley.

The fungal pathogen Bipolaris sorokiniana (teleomorph Cochliobolus sativus)causes the foliar disease spot blotch (SB) and the root disease common root rot (CRR). Spot blotch and CRR are serious disease constraints to barley production in warmer growing regions of the world, with estimated yield losses ranging from 30-70% from SB and 15-30% for CRR. Although chemical treatments may assist incontrolling spot blotch infections, the most effective and environmentally sound means of control for each disease is breeding for varieties with natural resistance. InAustralia, no commercially available varieties offer resistance to either SB or CRR. This study has sought to establish molecular markers that will be useful for selecting for resistance to each of these important fungal diseases.Barley cultivars derived from the breeding line NDB112 have provided durable SB resistance in the North Dakota region of the USA for over 40 years. The robustnessof this resistance had not been determined under Australian environmental conditions or with those B. sorokiniana pathotypes present within Australia. Toelucidate the genetics of resistance, two seedling and two field trials were conducted on an ND11231-12/VB9524 (ND/VB) doubled haploid (DH) population (180 lines).A molecular map of the ND/VB population was curated in order to provide a firm basis for mapping of resistance loci. Composite interval mapping revealed thatdifferent gene combinations are effective at different stages of plant development. Seedling resistance was found to be conditioned by a major locus on the short arm ofchromosome 7H and this region was validated in the related population ND11231-11/WI2875*17. A minor quantitative locus on chromosome 5HS was detected in one of the two seedling trials. However, this region requires further investigation to confirm its association to SB resistance in this population. Field resistance to SB in adult plants was found to be associated with two major quantitative trait loci (QTL)on chromosomes 7HS and 3HS; and a putative third minor QTL on chromosome 2HS. The 7H region is common between seedling and field resistance and is the most important locus for the expression of resistance at both stages of plant development. These findings largely concur with genetic studies of this trait in tworowed barley germplasm in North American environments.Common root rot is a difficult disease to phenotype for, and breeding programs will benefit from the identification of molecular markers linked to resistance. Data wasprovided from field trials of subsets of the population over four years. Using a novel approach combining the efficiency of bulked-segregant analysis with highthroughputDiversity Arrays Technology markers (BSA-DArT), CRR resistance was found to be conditioned by three putative QTL in an unmapped Delta/Lindwall population. QTL were identified on chromosomes 2HS, 4HS, and 7HS. To validatethe trait-linkage associations between the DArT markers and the CRR QTL,microsatellite (SSR) markers known to map to the regions identified by BSA-DArT were used. The 2H and 4H regions were validated using marker regression of the SSR markers in most seedling trials, whereas the 7H QTL, which is proximal to the location of the SB resistance QTL in the ND/VB population, was detected in only one seedling trial.The QTL identified in this study offer potential to combat the foliar and root diseases causes by this fungal pathogen. The chromosomal location of QTL for SB and CRR resistance have been found to differ in the ND/VB and D/L populations,which suggests that resistance to each disease is independently inherited. Further research is required to confirm the hypothesis that it is possible to combineresistance to both diseases into a single genotype. Such allelic combinations would provide elite germplasm that would benefit barley breeding programs world-wide.

Weather based models help time fungicide applications to the periods when the diseases are most likely to occur. The first objective of this work was to compare and adapt weather-based advisories developed for the control of Cercospora arachidicola on peanuts for resistant cultivars. It was achieved by comparing the disease progress curves of the 1997-1999 growing seasons in Lewiston NC, to spray schedules simulated by the Virginia Advisory, the Parvin, Smith and Crosby Advisory (PSC), NC Advisory, and AU-Pnuts Advisory and their adaptations for resistance. Field trials were conducted in 1997, 1998 and 1999 to test adaptations for resistant genotypes based on the NC Advisory. In all three years the leaf spot epidemics started late in the season (September). There was no yield difference due to leaf spot control except in 1999 in Lewiston for the susceptible genotypes (NC 7 and NC 11). All the advisories had a tendency to overspray at the beginning of the season, this might be due to a lack of inoculum at this time. The resistant genotype used for the study, GP-NC 343, did not lose any yield due to leaf spot in any of the tests and therefore did not need to be sprayed. The model that had the best fit to the disease progress curve of the susceptible genotypes was the AU-Pnuts 12/4. The AU-Pnuts advisory 7/3, currently used in the Southeastern US, started spraying to early in the season for NC. The Virginia advisories also oversprayed. The NC advisory and the PSC were considered almost equivalent, and the adaptations for the PSC did not differ from the PSC itself.The second objective was to develop a simulation model to predict epidemics of Stagonospora nodorum on winter wheat. The CERES-Wheat model was used to simulated leaf area indexes (LAI) for the wheat plant throughout the season. The disease model developed in this work simulated the spread of spores onto the plant leaves and heads, infection, the latent period and, lesion extension. The model equations were inferred from the literature and were calibrated with disease assessments made on Coker 9904 during the spring of 1998 in Plymouth NC. For 1998 and 1999, disease increase in the lower leaves took place 20 days after the disease increase was simulated by the model both years. The most effective spray timing corresponded to a period when disease was first observed in the lower leaves, no disease was seen on the flag leaf, and simulated onset of disease on the flag leaf had occurred. A sharp simulated disease increase in the flag leaf compartment may be a very good indicator for a spray recommendation. Combining a disease model to an already existing crop growth model facilitated modeling disease progress. Further work will be needed to fully validate both the CERES-wheat and the S. nodorum models in North Carolina Coastal Plains.

Upon sensing pathogens, plants initiating defense responses typically resulting in programmed cell death (PCD). PCD effectively subdues biotrophic pathogens but is hijacked by necrotrophs that colonize the resulting dead tissues. We showed that barley wall associated kinase (WAK) genes, underlying the rcs5 QTL, are manipulated by the necrotrophic fungal pathogen Bipolaris sorokiniana to cause spot blotch disease. The rcs5 genetic interval was delimited to ~0.23 cM, representing an ~234 kb genomic region containing four WAK genes, designated HvWak2, Sbs1, Sbs2, and HvWak5. Post-transcriptional gene silencing of Sbs1&2 in the susceptible barley cultivars Steptoe and Harrington resulted in resistance, suggesting a dominant susceptibility function. Sbs1&2 expression is undetectable in barley prior to pathogen challenge; however, specific upregulation of Sbs1&2 occurred in the susceptible lines post inoculation. Promotor sequence polymorphisms were identified in the allele analysis of Sbs1&2 from eight resistant and two susceptible barley lines, which supported the possible role of promotor regulation by virulent isolates contributing to susceptibility. Apoplastic wash fluids from virulent isolates induced Sbs1expression, suggesting regulation by an apoplastic-secreted effector. Thus, the Sbs1&2 genes are the first susceptibility/resistance genes that confer resistance against spot blotch, a disease that threatens barley and wheat production worldwide. The nec3 mutants of barley are hyper-susceptible to many necrotrophs and show distinctive cream to orange necrotic lesions that are induced by infection, representing aberrant PCD. The ?- irradiation induced necrotic mutant, nec3-?1 (Bowman) was confirmed as a nec3 mutant by allelism tests. The F2 progeny of a cross of nec3 x Quest inoculated with B. sorokiniana segregated as a single recessive gene fitting a 3 WT: 1 mutant ratio. The homozygous F2 mutant progeny were genotyped with four SSR and 25 SNP markers at nec3 locus on chromosome 6H, a physical region spanning ~ 16.96 Mb containing 91 high and low confidence annotated genes. Exome capture sequencing of nec3 mutants failed to identify a candidate gene, however, RNAseq analysis identified two candidates in the nec3 region with >three-fold downregulation. We hypothesize that the underlying aberrant PCD mechanism in the nec3 barley mutant facilitates extreme susceptibility to multiple adapted fungal pathogens of barley.

Spot blotch is one of the most important diseases of barley (Hordeum vulgare) in Libya and worldwide. The overall aim of this study was to investigate the potential of biological control in combination with disease resistance to control spot blotch without the potential hazards of chemical application. Fungi were isolated from barley plants with spot blotch symptoms from different areas in Libya. As well as the commonly known spot blotch pathogen Bipolaris sorokiniana (teleomorph Cochliobolus sativus), Bipolaris spicifera (teleomorph Cochliobolus spicifer), Curvularia inaequalis, and Alternaria alternata were identified by their morphology and ribosomal DNA sequences. Bipolaris sorokiniana was the most serious pathogen under the test conditions; the others infected barley but caused less severe symptoms. Spot blotch resistance of barley seedlings was tested under greenhouse conditions with four Libyan cultivars (ACSAD, Nibola, Rehan, and Wadi Utbah) and two UK cultivars (Gaelic and Pastoral). Nibola was the most resistant. The ability of the organisms in three commercial biocontrol products, Trichoderma harzianum T-22 (Trianum), Streptomyces lydicus WYEC 108 (Actinovate) and Bacillus subtilis QST 713 (Serenade), to control spot blotch individually and in combination was investigated. On agar plates, all three inhibited growth of the pathogens completely on the second day of culture, except that for B. spicifera with S. lydicus there was an inhibition zone and the pathogen grew in the opposite direction. Disease severity was lowest when T. harzianum T-22 was applied individually to the most resistant cultivar, Nibola. Foliar application, soil treatment and seed coating all reduced disease severity. With foliar application, T. harzianum T-22 was more effective when applied at the same time as the pathogen than when applied one week before or four days after. In a field experiment with T. harzianum T-22, foliar application combined with seed treatment suppressed spot blotch more effectively than either method individually.

Tan spot is an important disease caused by the necrotrophic fungus Pyrenophora triticirepentis. Two common necrotrophic effectors produced by this fungus are Ptr ToxA and Ptr ToxB, which recognize host sensitivity genes Tsn1 and Tsc2, respectively. In this research, a tetraploid recombinant inbred line population was evaluated for reaction to the Ptr ToxA and Ptr ToxB-producing isolates 86-124 (race 2) and DW5 (race 5). The results indicated that a compatible Tsc2-Ptr ToxB interaction accounted for 26% of the disease variation, which states that this interaction plays a significant role in the development of tan spot. On the contrary, the Tsn1-Ptr ToxA interaction was not associated with tan spot caused by 86-124. However, evaluation of a ToxA-producing isolate of Parastagonospora nodorum, indicated that the Tsn1- ToxA interaction accounted for 38% of the variation. Therefore, the Tsn1-ToxA interaction played a significant role in the development of septoria nodorum blotch, but not tan spot.

Pyrenophora teres f. maculata causes spot form net blotch (SFNB) on barley and was recently documented in North Dakota. The impact of SFNB on barley, the genetic diversity of the pathogen, and virulence structure are unknown for the state. Yield and quality loss in North Dakota due to SFNB was investigated over eleven year-sites, and simple linear regression of percent yield loss on adjusted percent disease using year-site means of treatments predicted a 0.77% increase in yield loss for every 1% increase in disease. When virulence of isolates of P. teres f. maculata collected from geographically diverse regions in the northern United States was evaluated on differential barley genotypes, few isolates were identical in terms of virulence patterns, and the virulence profile of a population from Idaho differed from other populations. To understand population structure and genetic diversity, SNPs of 140 isolates were generated using genotyping-by-sequencing for analysis of population genetics and structure. Evidence for sexual recombination in each population includes the ratio of mating-type idiomorphs that do not significantly differ from a 1:1 ratio; low index of association values for most populations; and high variation within and low variation among populations. Association mapping detected forty-five significant marker-trait associations of SNPs associated with virulence or avirulence across 19 P. teres f. maculata scaffolds using 82 isolates of P. teres f. maculata from diverse areas in the northern United States. The most significant marker, 01700_198, was found on P. teres f. maculata-scaffold 8 when the population was challenged with four different barley lines. This research demonstrates that SFNB causes significant yield loss; that high diversity exists in the pathogen, with respect to virulence and population genetics; and that association mapping can be used to identify virulence/avirulence marker-trait associations to fill gaps in our understanding of host-parasite genetic interactions in this pathosystem.
American Malting Barley Association
Triticeae CAP
North Dakota Barley Commission

Cultivated emmer wheat (Triticum turgidum ssp. dicoccum) is a good source of genes for resistance to several major diseases of wheat. The objectives of this study were to use genome-wide association analysis to detect genomic regions in cultivated emmer germplasm harboring novel resistance genes to four wheat diseases: stem rust, leaf rust, tan spot, and Septoria nodorum blotch (SNB). A natural population including 180 cultivated emmer accessions with a high level of geographic diversity was assembled as the association-mapping panel. This cultivated emmer panel was evaluated phenotypically by scoring reactions to stem rust, leaf rust, tan spot, and SNB and was genotyped using a 9K SNP Infinium array. After filtering for missing data points and minor allele frequency (MAF), 4,134 SNPs were used for association analysis using 178 emmer accessions. Based on principle component (PC) analysis, five subpopulations strongly associated with geographic origins were suggested by the first three PCs. Genome-wide association analysis revealed that 222, 42, 146, and 42 SNPs were significantly associated with resistance to stem rust, leaf rust, tan spot, and SNB, respectively, at the significant level of 1 percentile. Among the significant SNPs at the significant level of 0.1 percentile, ten, one, nine, and one co-located with known genes or QTL associated with resistance to the four diseases, respectively. The remaining significant SNPs were located in the genomic regions where no known resistance genes have been identified for the four diseases. This evidence suggests that some of the emmer wheat accessions carry novel genes conferring resistance to the four diseases. Additionally, 14, three, eight, and five LD blocks harboring at least one significant SNP were identified and might harbor putative QTL related to resistance to the four diseases, respectively. These studies provide information about the genomic regions in cultivated emmer that are associated with resistance to stem rust, leaf rust, tan spot, and SNB. Results from these studies provide guidance for selecting emmer accessions when decisions are being made about the parents that will be used for the development of new resistant germplasm and mapping populations for identifying novel genes conferring resistance to major wheat diseases.
Chinese Scholarship Council (CSC)

Three independent studies were conducted for spot blotch (Bipolaris sorokiniana), Fusarium head blight (FHB) (Fusarium graminearum), and root rot diseases (Fusarium species and B. sorokiniana). Histopathology of compatible and incompatible interactions between different pathotypes of B. sorokiniana and different genotypes of barley was examined with red fluorescent protein-tagged fungal isolates. The fungus penetrated the host cell wall and developed multicellular globular infection hyphae (IH) in the lumen of epidermal cells, but infected epidermal cells appeared to be alive till 16 hours post-inoculation (HPI). In the susceptible plants, the tip of IH was found to grow ahead of the dead tissue and invade the surrounding live mesophyll cells, whereas growth of IH in the resistant plants was restricted to the dead tissue after 20 HPI. The amount of H2O2 accumulation and the fungal biomass were also significantly higher in the susceptible hosts than in the resistant hosts. To map resistance to FHB, two populations consisting 130 doubled haploid lines from the cross Grandin ? PI277012 and 237 recombinant inbred lines from the cross Bobwhite ? ND2710 were phenotyped and genotyped. QTL for Type I resistance were identified on chromosomes 1A, 2B, 4B, 5B and 6B in the GP population. These QTL explained 10.7-19 % of the total phenotypic variation. With the BN population, QTL for Type I resistance were identified on chromosomes 2A, 5A and 6B, explaining 6.2-13.7% of the total phenotypic variation. To assess the prevalence, incidence and severity of wheat crown rot (CR) and common root rot (CRR) in ND, wheat root samples were collected from fields across the state in 2012, 2013, and 2014. Fungal isolations indicated that B. sorokiniana was most frequently recovered in all sampled years. Seedling tests on ten spring wheat lines showed that Glenn was the least susceptible while Steele-ND was the most susceptible to one F. culmorum isolate and one B. sorokiniana isolate tested. Evaluation of 20 spring wheat genotypes for reaction to CRR at the adult plant stage showed that Freyr and RB07 were more resistant while Len and Briggs were more susceptible to CRR compared to other wheat genotypes evaluated.
North Dakota Wheat Commission,
Minnesota Wheat Research and Promotion Council
ND State Board of Agricultural Research and Education
Triticeae-CAP project (2011-68002-30029) of the US Department of Agriculture National Institute of Food and Agriculture
U.S. Wheat and Barley Scab Initiative (USWBSI)

Dissertation (PhD(Agric)) -- University of Stellenbosch, 2001.
ENGLISH ABSTRACT: Net blotch of barley, caused by Pyrenophora teres, is one of the most important diseases of this cereal in the south Western Cape Province of South Africa. This fungus exists as two different types (forms), namely a nettype and a spot-type that are distinguished by differential symptom expression on barley leaves. Based on this specific plant pathological difference a series of studies of agricultural importance were executed to investigate the effects of sexual recombination between these two types. In addition, studies were done to determine the difference between local net- and spot-type populations with regards to population structure and fungicide sensitivity. This dissertation therefore, consists of a collection of separate publications and as a result a certain degree of redundancy has been unavoidable. Recombination is one of the most important evolutionary forces involved with sexual reproduction. In plant-fungal agricultural ecosystems this may result in pathogenic fungal populations adapting more rapidly to control programs such as fungicide applications. The first section of the review in part 1 of this dissertation covers different aspects of sexual reproduction in ascomycetes, specifically focussing on mating-type genes, vegetative incompatibility and recombination. The major part of the review is then dedicated to various plant pathological aspects of P.teres, specifically addressing the differences between the two types, and in various cases highlighting the significance of sexual recombination within and between the net- and spot-type. Using morphological criteria for identification purposes there have been many conflicting reports concerning the identity of leaf spot isolates in the Western Cape Province of South Africa. In part 2, the correct identity was eventually achieved employing mating studies and molecular markers .: This was accomplished after single ascospores were obtained from pseudothecia after in vitro mating had occurred between a verified P. teres net-blotch isolate from Denmark and a representative Pyrenophora leaf spot isolate from South Africa. Using amplified fragment length polymorphism (AFLP) and RAPD markers, recombination was demonstrated in the progeny that had DNA banding patterns different from the two parental isolates. Pathogenicity trials also confirmed that recombination had taken place during mating. Inoculations were conducted on the differential cultivars susceptible to the net-blotch and leaf spot forms. The two parents induced typical net-blotch or leaf spot symptoms whereas the progeny mostly induced a jagged spot symptom on each cultivar. Fungicide sensitivity tests using the ergosterol biosynthesis inhibitors showed that, due to recombination, some progeny could have increased resistance to these fungicides. Due to mating and subsequent recombination between a net blotch isolate of P. teres and a representative leaf spot isolate, it was concluded that the latter was P. teres f. maculata. Fifteen of the net-spot hybrid progeny (F1) produced from the mating study in Part 2 were screened in Part 3 to assess their viability and genetic stability. Hybrid progeny (F1) inoculated onto barley seedlings consisting of the cultivars Stirling (differentially susceptible to net-type isolates), B87/14 and Clipper (both differentially susceptible to spot-type isolates) produced intermediate symptoms on all cultivars. Axenic cultures (F1-1) isolated from foliar lesions, followed by repeated inoculation and isolation (F1-2) onto a healthy set of seedlings produced similar intermediate symptoms. RAPDs conducted with two 1Q-mer primers on all isolates of F1-1and F1-2progeny revealed profiles similar to those obtained for F1 isolates. RAPD molecular data, therefore, indicated that hybrid progeny of this net x spot mating were genetically stable after having been subjected to two repetitive inoculation and reisolation cycles. Phylogenetic analysis of DNA sequences of the internal transcribed spacers (ITS1 and ITS2) flanking the 5.8S nuclear ribosomal RNA gene and the 5' end partial histone-3 gene confirmed the genetic stability of the hybrid progeny. These results also indicated that the hybrid progeny produced consistent symptoms throughout the series of experiments, and maintained their virulence to the differential cultivars screened. Both types of P. teres are prevalent in the south Western Cape Province of South Africa, found on susceptible cultivars often grown within close proximity of each other. In Part 4, a net- and spot-type population were characterised in terms of their population structure using RAPD markers. Samples were collected from infected barley leaves from two separate quadrants in each field, the two quadrants positioned in corners of the fields, diagonal to one another. A total of 65 loci were produced of which 54 were polymorphic. Total gene diversities determined for all loci resulted in mean indices of 0.063 and 0.082 being obtained respectively for the net- and spottype populations. A coefficient of genetic differentiation (Gs) of 0.0149 was obtained between sites within populations while a coefficient (GT) of 0.63 was obtained between the two populations. Genotypic variation revealed 13 distinct multilocus genotypes (haplotypes) in the net-type population while there were 12 in the spot-type population. UPGMA cluster analysis done on the two populations together with six progeny from the mating between a netand spot-type isolate resulted in three main clusters being produced, one for each population and one for the progeny. One isolate collected from the nettype population also contained a unique spot-type RAPD fragment. This suggested that sexual recombination may be taking place between isolates of the net- and spot-type under field conditions. Fungicide application is the most important method used in the control of net blotch in South Africa. In Part 5 the fungicide sensitivities (ICsD values) of 89 monoconidial isolates (46 net-type and 43 spot-type) of P. teres to sterol demethylation inhibiting fungicides were determined, based on the inhibitory effect on radial mycelial growth. The fungicides evaluated were triadimenol, bromuconazole, flusilazole, propiconazole and tebuconazole. Both net- and spot-type isolates revealed strong resistance to triadimenol while flusilazole was shown to be the strongest inhibitor of fungal growth. Spot-type isolates showed a higher resistance than net-type isolates to all five fungicides screened. The ICsD values indicated significant differences between four of the fungicides (triadimenol, tebuconazole, flusilazole and propiconazole). The ICsD values between propiconazole and bromuconazole were not significant. This study suggested that spot-type isolates showed a higher degree of resistance to commercially used fungicides than net-type isolates. The overall conclusion of this study is that the spot-type of P. teres is the pathogen associated with leaf spots of barley in the south western Cape province of South Africa and not P. japonica as earlier reported. Together with the net-type, both types exist as genetically variable populations in this barley production region. Mating between the two types results in sexual progeny that are genetically stable. This implies that barley fields adjacent to one another in which either net- or spot-type susceptible cultivars are being cultivated may lead to sexual progeny being produced. This in turn may lead to an increased rate at which fungal populations may become resistant to commercially used fungicides. It is furthermore suggested that an alternative fungicide seed treatment is used instead of triadimenol due to high resistance of P. teres to this fungicide.
AFRIKAANSE OPSOMMING: Netvlek op gars is een van die belangrikste siektes van hierdie graansoort in die suidelike deel van die Westelike Kaapprovinsie. Dié siekte word veroorsaak deur die swam Pyrenophora teres. Hierdie swam kom voor as twee verskillende tipes, naamlik 'n net-tipe en 'n kol-tipe wat onderskei word op grand van die voorkoms van hulle simptome op garsblare. Hierdie planpatologiese verskil in ag genome, is 'n reeks studies van landboukundige waarde uitgevoer om die effek van geslagtelike rekombinasie tussen die twee tipes te ondersoek. Daarbenewens is ook studies uitgevoer om om die verskil te bepaal tussen plaaslike net- en koltipe populasies ten opsigte van populasiestruktuur en fungisiedsensitiwiteit. Hierdie verhandeling bestaan dus uit 'n versameling afsonderlike publikasies en as gevolg daarvan is daar onvermydelik'n mate van oorvleueling. Rekombinasie is een van die belangrikste evolusionêre kragte betrokke by geslagtelike voortplanting. In plant-swam landboukundige ekostelsels kan dit veroorsaak dat patogene swampopulasies vinniger aanpas by beheerpragramme soos fungisiedtoediening. Die eerste gedeelte in deel 1 van hierdie verhandeling dek die verskillende aspekte van geslagtelike voortplanting van ascomycetes, met spesifieke verwysing na paringstipe gene, vegetatiewe onverenigbaarheid en rekombinasie. Die grootste gedeelte van die oorsig word gewyaan verskeie plantpatologiese aspekte van P. teres,en wys veralop die verskille tussen die twee tipes. In verskeie gevalle word die betekenis van geslagsrekombinasie binne en tussen die net- en koltipe uitgelig. Deur morfologiese kenmerke vir identifikasiedoeleindes te gebruik, is daar baie teenstrydige verslae rakende die identifikasie van blaarvlekisolate in die Westlike Kaapprovinsie van Suid-Afrika. In deel 2 is die korrekte identifikasie eventueel verkry deur gebruik te maak van paringstudies en molekulêre merkers. Dit is bereik nadat enkel ascospore verkry is uit pseudothecia gevorm na in vitro paring plaasgevind het tussen 'n bevestigde P. teres netvlek isolaat uit Denemarke en 'n verteenwoordigende Pyrenophora blaarvlekisolaat van Suid- Afrika. Deur gebruik te maak van versterkte fragmentlengte polimorfisme [AFLP] en RAPD merkers, is rekombinasie gedemonstreer in die nasate wat DNA bandpatrone gehad het wat verskil het van dié van die "ouer" isolate. Patogenisiteitstoetse het ook bevestig dat rekombinasie tydens paring plaasgevind het. Inokulasies is uitgevoer op die verskillende cultivars wat vatbaar is vir die netvlek en blaarvlek vorme. Die twee ouers het tipiese netvlek of blaarvlek simptome veroorsaak, terwyl die nasate hoekige vlekke veroorsaak het op elke cultivar. Toetse vir fungisiedsensitiwiteit deur gebruik van die ergosterol biosintese inhibeerders het gewys dat a.g.v. rekombinasie sekere nasate verhoogde weerstand teen hierdie fungisiedes het. As gevolg van paring en daaropvolgende rekombinasie tussen 'n netvlek isolaat van P. teres en 'n verteenwoordigende blaarvlek isolaat is afgelei dat laasgenoemde P. teres f. maculata is. Vyftien van die netvlek hibried nakomelinge (F1) verkry van die paringstudie in deel 2 is ondersoek in deel 3 om hul lewensvatbaarheid en genetiese stabiliteit te bepaal. Hibried nasate (F1) geïnokuleer op garssaailinge bestaande uit die volgende cultivars: Stirling (soms vatbaar vir net-tipe isolate) , B87/14 en Clipper (albei soms vatbaar vir kol-tipe isolate) het intermediêre simptome op al die cultivars veroorsaak. Akseniese kulture (F1-1) geïsoleer uit blaarletsels gevolg deur herhaalde inokulasie en isolasie (F1-2) op 'n gesonde stel saailinge het dieselfde intermediêre simptome veroorsaak. RAPDs uitgevoer met twee 10-mer inleiers op al die isolate van F1-1 en F1-2 nasate het profiele opgelewer soortgelyk aan dié wat vir F1 isolate verkry is. RAPD molekulêre data het dus gewys dat die hibried nasate van hierdie net x kol paring geneties stabiel was nadat dit onderwerp is aan twee inokulasie en reïsolasie siklusse. Genetiese stabiliteit van die hibried nageslag is bevestig deur filogenetiese analise van die DNA volgorde van die interne getranskribeerde spasieerders (ITS1 en ITS2) reg langs die 5.8S nukluêre ribosomale RNA geen en die 5' end gedeeltelike histoon-3 geen. Hierdie resultate het ook gewys dat die hibried nasate konstante simptome getoon het tydens die hele reeks eksperimente en hulle virulensie behou het vir die kultivars wat getoets is. Beide tipes van P. teres kom algemeen voor in die suidelike deel van die Westelike Kaapprovinsie en word gevind op vatbare cultivars wat dikwels naby mekaar groei. In deel 4 is 'n net- en kol-tipe populasie gekarakteriseer in terme van hulle populasiestruktuur deur gebruik van RAPD merkers. Monsters is versamel van geïnfekteerde garsblare van twee aparte kwadrante in elke saailand. Die twee kwadrante is geplaas in die hoeke van die saailand, diagonaal tot mekaar. 'n Totaal van 65 lokusse is gevorm, waarvan 54 polimorfies was. Die algehele genetiese verskeidenheid bepaal vir alle lokusse, het gelei tot gemiddelde indekse van 0.063 en 0.082 soos gevind vir die net- en kol-tipe populasies. 'n Koëffisiënt van genetiese differensiasie (Gs ) van 0.0149 is gevind tussen gebiede tussen populasies, terwyl 'n koëffisiënt (GT) van 0.63 gevind is tussen die twee populasies. Genotipiese variasie het 13 duidelike multilokus genotipes (haplotipes) getoon in die net-tipe populasie, terwyl daar twaalf was in die kol-tipe populasie. UPGMA groeperingsanalises wat gedoen is op die twee populasies tesame met ses nasate van die paring van 'n net- en koltipe isolaat het tot gevolg gehad dat drie hoof groepe gevorm is, een vir elke populasie en een vir die nasate. Een isolaat wat versamel is, van die net-tipe populasie het 'n unieke kol-tipe RAPD fragment bevat. Dit wys daarop dat geslagtelike rekombinasie in veldomstandighede mag voorkom tussen isolate van die net- en kol-tipe. Fungisiedtoediening is die belangrikste metode wat gebruik word om netvlek in Suid-Afrika te beheer. In deel 5 is die fungisiedsensitiwteit (Ieso waardes) van 89 enkelkonidiale isolate (46 net-tipe en 43 kol-tipe) van P. teres teen sterol demetielasie inhiberende fungisiedes bepaal, op die basis van die onderdrukkende effek op die radiale groei van die miselium. Die volgende fungisiedes is geëvalueer: triadimenol, bromuconazole, flusilazole, propiconazole en tebuconazole. Beide net- en kol-tipe isolate het 'n sterk weerstand teen triadimenol openbaar, terwyl flusilazole gevind is as die sterkste onderdrukker van swamgroei. Kol-tipe isolate het 'n hoër weerstand as die net-tipe isolate teen al vyf fungisiedes wat getoets is, gehad. Die lesowaardes het aangedui dat daar beduidende verskille tussen vier van die fungisiedes IS (triadimenol, tebuconazole, flusilazole en propiconazole). Die leso waardes tussen propiconazole en bromuconazole was nie beduidend nie. Die gevolgtrekking van hierdie studie is dus dat die kol-tipe isolate 'n hoër graad van weerstand teen kommersiëel gebruikte fungisiedes as die net-tipe isolate gehad het. Die algehele gevolgtrekking van hierdie studie is dat die kol-tipe van P. teres, die patogeen is wat geassosieer word met blaarvlekke op gars in die suidwestelike Kaapprovinsie van Suid-Afrika, en nie P. japonica soos voorheen gerapporteer nie. Tesame met die net-tipe, kom altwee tipes voor as geneties veranderlike populasies in hierdie gars verbouingstreek. Paring tussen die twee tipes lei tot geslagtelike nasate wat geneties stabiel is. Dit impliseer dat aangrensende garsvelde waarop net- óf kol-tipe vatbare kultivars verbou word, mag lei tot die produksie van geslagtelike nasate. Dit kan weer lei tot 'n verhoogde tempo waarteen swampopulasies weerstandbiedend teenoor kommersiële fungisiedes raak. Daar word verder ook voorgestel dat alternatiewe fungisied saadbehandelings gebruik word in plaas van triadimenol as gevolg van verhoogde weerstand van P. teres teenoor laasgenoemde.

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Brown spot (Bipolaris sorokiniana) and net blotch (Drechslera teres) are the main foliar diseases of barley in southern of Brazil. The objectives of this study were to evaluate the survival and viability of B. sorokiniana and D. teres on barley seeds during the off seasons and verify the influence of different barley seed treatments on: a. The fungi transmission to plants; b. The population of emerged plants; c. The brown spot and net-blotch intensity; d. The productivity; e. The seed health and f. the number of captured spores in the air. Barley seeds of six cultivars from four regions were used for viability analysis. Seed sanity tests were developed during ten months. Samples of 400 seeds were disinfected by sodium hypochlorite (4%) and whashed by sterile distilled water. The samples were plated in the Potato Dextrose Agar medium and incubated in growth chamber for seven to ten days. Field experiments were carried out with two barley cultivars sowed in two different dates of 2012 and 2013 growing season. The experiments were performed with treatments, control (without fungicide seed treatment), commercial treatment and additional laboratory treatments. The experiments were conducted in a randomized block design with four replications. The number of symptomatic plants with brown spot and net-blotch diseases was assessed from five to seven days intervals during 40 days. The incidence and severity foliar were quantified from 40 to 95 days after sowing in ten tillers randomly per plot. Collectors spores like windmill with microscope slide smeared with a mixture of phenol + hexane + Vaseline + paraffin were installed in the field. The microscope slides were changed weekly, and the collectors remained in the field up to the 38 plant ear emergence. Grain yield, grain classification and thousand kernels was assessed during the harvest and the seeds submitted to pathology test. The incidence and viability of B. sorokiniana and D. teres reduced with the storage. The viability average reduction of B. sorokiniana and D. teres was 27% and 30% in the off season, respectively. None of the simultaneus seed treatments eradicated the fungi. Seed treatments allowed fungi transmission to the plant leaves. Seeds commercial treatment was not effective in the fungi eradication, allowing up to 90% transmission to plants. Additional seed treatments reduced up tp 89% the fungi transmission. Commercial seed treatment showed the AUDPCS of 519.0 and 139.0 for net blotch and brown spot, respectively. The most efficient seed treatment was triadimenol + difenoconazol + carbendazim + thiamethoxam, reducing the B. sorokiniana and D. teres AUSPC in 11.4 and 120.5, respectively. The highest fungi conidia capturing occurred in field under commercial treatment seeds. There was a positive and significant correlation (r = 0.89 B. sorokiniana and r = 0.70 D. teres) between the number of spores in the air and severity. Seed treatment influenced the sanitary quality of barley seeds. There is a significant and positive correlation (r = 0.99) between the brown spot and net-blotch AUDPC and the incidence of B. sorokiniana and D. teres in the harvested seed. Commercial seed treatment did not reduce the B. sorokiniana and D. teres inocula in barley seeds. Additional, commercial seed treatment anticipated the begining of brown spot and net blotch epidemic, increasing yield costs. All other treatments did not eradicate the fungi. However, they delayed the bigining of diseases, enabling the farmer profit increase
A mancha-marrom (Bipolaris sorokiniana) e a mancha-em-rede (Drechslera teres) são as principais doenças fúngicas foliares da cevada no sul do Brasil. Os objetivos foram: quantificar a sobrevivência e a viabilidade de B. sorokiniana e D. teres em sementes de cevada durante a entressafra e verificar o efeito de diferentes tratamentos de sementes de cevada na transmissão dos fungos para a parte aérea das plantas, na população de plantas emersas, na intensidade da mancha marrom e da mancha-em-rede, na produtividade, na sanidade de sementes colhidas e no número de conídios capturados no ar. Foram utilizadas sementes de cevada de seis cultivares oriundas de quatro regiões para a análise de viabilidade. Os testes de sanidade foram desenvolvidos durante dez meses. Amostras de 400 sementes foram desinfestadas em hipoclorito de sódio (4%) e água destilada esterilizada, distribuídas em meio de cultura Batata-Dextrose-Ágar e encubadas em câmara de crescimento durante sete a dez dias. Os experimentos de campo foram conduzidos em 2012 e 2013 em duas épocas de semeadura e duas cultivares. Os tratamentos utilizados foram: testemunha (sem tratamento fungicida de sementes), tratamento comercial e tratamentos adicionais testados em laboratório. O modelo experimental foi em blocos casualisados e quatro repetições. O número de plantas sintomáticas com mancha marrom e mancha-em-rede foi quantificado em intervalos de cinco a sete dias até 40 dias após a semeadura. A incidência e a severidade foliar foram quantificadas desde os 40 até os 95 dias após a semeadura em dez perfilhos coletados ao acaso de cada parcela. Foram instalados coletores de esporos tipo cata-vento contendo lâmina de microscopia untada com mistura de fenol+hexano+vaselina+parafina. As lâminas foram trocadas semanalmente, e os coletores permaneceram no campo até o espigamento das plantas. Na colheita, foi quantificado rendimento de grãos, classificação e massa de mil grãos. As sementes colhidas foram submetidas ao teste de sanidade de sementes. Houve redução da incidência e da viabilidade de B. sorokiniana e D. teres com o armazenamento. A redução média viabilidade de B. sorokiniana e D. teres foi de 27% e 30% na entressafra, respectivamente. Nenhum dos tratamentos erradicou os fungos simultaneamente das sementes e possibilitaram transmissão para a parte aérea as plantas. O tratamento comercial de sementes não foi eficiente na erradicação dos patógenos com transmissão para a parte aérea de até 90%. Tratamentos de sementes adicionais reduziram a transmissão dos fungos em até 89%. O tratamento comercial de sementes antecipou as doenças foliares com AACPS de até 519,0 de mancha em rede e 139,0 de mancha marrom. O tratamento de semente mais eficiente (triadimenol + difenoconazol + carbendazim + tiametoxan) reduziu a AACPS das doenças em 11,4 e 120,5, respectivamente. A maior captura de conídios no ar dos fungos ocorreu nas plantas submetidas ao tratamento comercial de sementes havendo correlação positiva e significativa (r = 0,89 B. sorokiniana e r = 0,70 D. teres) entre número de conídios no ar e severidade das doenças. O tratamento de semente utilizado influenciou a qualidade sanitária das sementes de cevada produzidas, com correlação significativa e positiva (r=0,99) entre AACPS da mancha marrom e da mancha-em-rede e incidência de B. sorokiniana e D. teres nas sementes colhidas. O tratamento comercial de sementes não reduziu o inóculo de B. sorokiniana e D. teres das sementes de cevada, antecipa a epidemia da mancha marrom e mancha-em-rede e aumenta custo de produção. Os demais tratamentos apesar de não erradicar os fungos, retardam as doenças e aumentam o lucro do agricultor

Outre la qualité de ses images, l'atout majeur du système SPOT est sa précision géométrique. Cette étude a pour objet d'évaluer l'apport d'une triangulation de bloc au traitement géométrique d'images SPOT, afin de traiter globalement la géométrie de l'ensemble des images d'un chantier de spatiocartographie. Tant l'intérêt réel du calcul de blocs que sa faisabilité sont ici démontrés. Au préalable, il est indispensable d'analyser la modélisation de la prise de vues SPOT. Une modélisation simple, robuste et proche de la réalité physique de la prise de vues SPOT est proposée. Avec un nombre restreint de points d'appui, l'exactitude obtenue reste inférieure à 10 mètres en planimétrie et 6 mètres en altimétrie, ce qui est conforme à la plupart des standards de cartographie à l'échelle du 1:50000. Sans point d'appui, une précision de 15 mètres a été obtenue sur des blocs couvrant 200 km x 200 km
Besides its image quality, the main advantage of Sport system is its geometric accuracy. The aim of this study is the evaluation of what block adjustment can bring to Spot images geometric processing, in order to process together all the images of a space mapping project. The actual advantage of this kind of computation is shown here. Before, a model of the image-ground relationship must be given. The proposed model is simple, robust and close to the physical image acquisition. With very few control points, the resulting accuracy is less than 10 meters in planimetry, and 6 meters in altimetry. These figures fit most standards of 1:50000 scale topographic maps. Without any ground control point, the accuracy is 15 meters on 200 km x 200 km area

Includes bibliographical references (leaves 106-111)
In recent years, studies have shown that 80-85% of e-mails sent were spam. Another form of spam that has just surfaced is VoIP (Voice over Internet Telephony) spam. Currently, VoIP has seen an increasing numbers of users due to the cheap rates. With the introduction of the IMS (IP Multimedia Subsystem), the number of VoIP users are expected to increase dramatically. This calls for a cause of concern, as the tools and methods that have been used for blocking email spam may not be suitable for real-time voice calls. In addition, VoIP phones will have URI type addresses, so the same methods that were used to generate automated e-mail spam messages can be employed for unsolicited voice calls. Spammers will always be present to take advantage of and adapt to trends in communication technology. Therefore, it is important that IMS have structures in place to alleviate the problems of spam. Recent solutions proposed to block SPIT (Spam over Internet Telephony) have the following shortcomings: restricting the users to trusted senders, causing delays in voice call set-up, reducing the efficiency of the system by increasing burden on proxies which have to do some form of bayesian or statistical filtering, and requiring dramatic changes in the protocols being used. The proposed decoying system for the IMS fits well with the existing protocol structure, and customers are oblivious of its operation.

Avec l'essor des objets connectés pour fournir un suivi systématique, objectif et fiable aux sportifs et à leur entraineur, de plus en plus de paramètres sont collectés pour un même individu. Une alternative aux méthodes d'évaluation en laboratoire est l'utilisation de capteurs inertiels qui permettent de suivre la performance sans l'entraver, sans limite d'espace et sans procédure d'initialisation fastidieuse. Les données collectées par ces capteurs peuvent être vues comme des données fonctionnelles multivariées : se sont des entités quantitatives évoluant au cours du temps de façon simultanée pour un même individu statistique. Cette thèse a pour objectif de chercher des paramètres d'analyse de la locomotion du cheval athlète à l'aide d'un capteur positionné dans la selle. Cet objet connecté (centrale inertielle, IMU) pour le secteur équestre permet de collecter l'accélération et la vitesse angulaire au cours du temps, dans les trois directions de l'espace et selon une fréquence d'échantillonnage de 100 Hz. Une base de données a ainsi été constituée rassemblant 3221 foulées de galop, collectées en ligne droite et en courbe et issues de 58 chevaux de sauts d'obstacles de niveaux et d'âges variés. Nous avons restreint notre travail à la prédiction de trois paramètres : la vitesse par foulée, la longueur de foulée et la qualité de saut. Pour répondre aux deux premiers objectifs nous avons développé une méthode de clustering fonctionnelle multivariée permettant de diviser notre base de données en sous-groupes plus homogènes du point de vue des signaux collectés. Cette méthode permet de caractériser chaque groupe par son profil moyen, facilitant leur compréhension et leur interprétation. Mais, contre toute attente, ce modèle de clustering n'a pas permis d'améliorer les résultats de prédiction de vitesse, les SVM restant le modèle ayant le pourcentage d'erreur inférieur à 0.6 m/s le plus faible. Il en est de même pour la longueur de foulée où une précision de 20 cm est atteinte grâce aux Support Vector Machine (SVM). Ces résultats peuvent s'expliquer par le fait que notre base de données est composée uniquement de 58 chevaux, ce qui est un nombre d'individus très faible pour du clustering. Nous avons ensuite étendu cette méthode au co-clustering de courbes fonctionnelles multivariées afin de faciliter la fouille des données collectées pour un même cheval au cours du temps. Cette méthode pourrait permettre de détecter et prévenir d'éventuels troubles locomoteurs, principale source d'arrêt du cheval de saut d'obstacle. Pour finir, nous avons investigué les liens entre qualité du saut et les signaux collectés par l'IMU. Nos premiers résultats montrent que les signaux collectés par la selle seuls ne suffisent pas à différencier finement la qualité du saut d'obstacle. Un apport d'information supplémentaire sera nécessaire, à l'aide d'autres capteurs complémentaires par exemple ou encore en étoffant la base de données de façon à avoir un panel de chevaux et de profils de sauts plus variés
With the growth of smart devices market to provide athletes and trainers a systematic, objective and reliable follow-up, more and more parameters are monitored for a same individual. An alternative to laboratory evaluation methods is the use of inertial sensors which allow following the performance without hindering it, without space limits and without tedious initialization procedures. Data collected by those sensors can be classified as multivariate functional data: some quantitative entities evolving along time and collected simultaneously for a same individual. The aim of this thesis is to find parameters for analysing the athlete horse locomotion thanks to a sensor put in the saddle. This connected device (inertial sensor, IMU) for equestrian sports allows the collection of acceleration and angular velocity along time in the three space directions and with a sampling frequency of 100 Hz. The database used for model development is made of 3221 canter strides from 58 ridden jumping horses of different age and level of competition. Two different protocols are used to collect data: one for straight path and one for curved path. We restricted our work to the prediction of three parameters: the speed per stride, the stride length and the jump quality. To meet the first to objectives, we developed a multivariate functional clustering method that allow the division of the database into smaller more homogeneous sub-groups from the collected signals point of view. This method allows the characterization of each group by it average profile, which ease the data understanding and interpretation. But surprisingly, this clustering model did not improve the results of speed prediction, Support Vector Machine (SVM) is the model with the lowest percentage of error above 0.6 m/s. The same applied for the stride length where an accuracy of 20 cm is reached thanks to SVM model. Those results can be explained by the fact that our database is build from 58 horses only, which is a quite low number of individuals for a clustering method. Then we extend this method to the co-clustering of multivariate functional data in order to ease the datamining of horses’ follow-up databases. This method might allow the detection and prevention of locomotor disturbances, main source of interruption of jumping horses. Lastly, we looked for correlation between jumping quality and signals collected by the IMU. First results show that signals collected by the saddle alone are not sufficient to differentiate finely the jumping quality. Additional information will be needed, for example using complementary sensors or by expanding the database to have a more diverse range of horses and jump profiles

Recently, the comparison of “periodized” strength training methods has been a focus of both exercise and sport science. Daily undulating periodization (DUP), using daily alterations in repetitions, has been developed and touted as a superior method of training, while block forms of programming for periodization have been questioned. Therefore, the purpose of this study is to compare block to DUP in Division I track and field athletes. Thirty-one athletes were assigned to either a 10-wk block or DUP training group in which sex, year, and event were matched. Over the course of the study, there were 4 testing sessions, which were used to evaluate a variety of strength characteristics. Although performance trends favored the block group for strength and rate of force development, no statistically significant differences were found between the 2 training groups. However, statistically different (P ≤ .05) values were found for estimated volume of work (volume load) and the amount of improvement per volume load between block and DUP groups. Based on calculated training efficiency scores, these data indicate that a block training model is more efficient than a DUP model in producing strength gains.

The effects of short-term resistance training on performance and health variables associated with prolonged sedentary lifestyle and metabolic syndrome (MS) were investigated. Resistance training may alter a number of health-related, physiological, and performance variables. As a result, resistance training can be used as a valuable tool in ameliorating the effects of a sedentary lifestyle including those associated with MS. Nineteen previously sedentary subjects (10 with MS and 9 with nonmetabolic syndrome [NMS]) underwent 8 weeks of supervised resistance training. Maximum strength was measured using an isometric midthigh pull and resulting force-time curve. Vertical jump height (JH) and power were measured using a force plate. The muscle cross-sectional area (CSA) and type were examined using muscle biopsy and standard analysis techniques. Aerobic power was measured on a cycle ergometer using a ParvoMedics 2400 Metabolic system. Endurance was measured as time to exhaustion on a cycle ergometer. After training, maximum isometric strength, JH, jump power, and V_ O2peak increased by approximately 10% (or more) in both the metabolic and NMS groups (both male and female subjects). Over 8 weeks of training, body mass did not change statistically, but percent body fat decreased in subjects with the MS and in women, and lean body mass increased in all groups (p # 0.05). Few alterations were noted in the fiber type. Men had larger CSAs compared those of with women, and there was a fiber-specific trend toward hypertrophy over time. In summary, 8 weeks of semiblock free-weight resistance training improved several performance variables and some cardiovascular factors associated with MS

In this paper, a simulation study is carried out for the development of concepts to optimize the tribological contact of valve plate and cylinder block in an axial piston machine in swash plate design. The valve plate/cylinder block contact is one of the three essential tribological contacts in axial piston machines. In a research project at the Institute for Fluid Power Drives and Systems (ifas), this contact is investigated by a specifically designed simulation tool. In addition, a test rig exists for the experimental investigation. With the results of simulation and experiment, it was shown before that the cylinder block is tilting to the high pressure side. Due to this movement, the gap height is not constant. In the area of minimum gap height, not only the fluid friction, but also the danger of solid body friction increases. Because of the higher friction losses in the area of minimum gap height, the temperature increase reduces the lifetime of the leaded coatings. In this paper, the results of the measurements as well as the simulation model are briefly summarized. It is followed by a simulation study of different possibilities to raise the gap height. Based on this pre-study, a first concept for the optimization of the tribological contact valve plate/cylinder block is presented and its applicability is discussed.

Daily undulating periodization (DUP), using daily alterations in repetitions, has been advocated as a superior method of resistance training, while traditional forms of programming for periodization (Block) have been questioned. Nineteen Division I track and field athletes were assigned to either a 10-week Block or DUP training group. Year and event were controlled. Over the course of the study, there were four testing sessions, which were used to evaluate a variety of strength characteristics, including maximum isometric strength, rate of force development, and one repetition maximum (1RM). Although, performance trends favored the Block group for strength and rate of force development, no statistical differences were found between the two groups. However, different (p ≤ 0.05) estimated volumes of work (VL) and amounts of improvement per VL were found between groups. Based upon calculated training efficiency scores, these data indicate that a Block training model is more efficient in producing strength gains than a DUP model. Additionally, alterations in testosterone (T), cortisol (C) and the T:C ratio were measured. Although there were no statistically (p ≤ 0.05) different hormone alterations between groups, relationships between training variables and hormone concentrations including the T:C ratio, indicate that Block may be more efficacious in terms of fatigue management.

The area of town part called Ring is situateted in Brno surrounded by Cejl, Křenová, Vlhká and Kolište Street. The main aim of the urban design is to provide the complete rehabilitation of the urban structure. The incompletely and accidentally arranged build- up area with many sandlots should be replaced with the traditional compact blocks which enable the create streets, boulevards, roads, green belts and a new piazetta. This design divides the border between private and public space. It counts with the replacement of the railway and bus station. There is designed the urban block with the dominance of the building function between the Cejl and Vlhká Street in the close neighborhood of old Edison factory. Another part of this block is created by the group of public buildings- Sport centre and Art school. There is also a hotel and an administrative building connecting few magistracies in the one place. The sport centre is divided into seven storeys respecting the fact that some storeys are 9 metres high if it requires the character of the sport space. There is a volume suitable for the basketball hall which can be reached from the entrance or from the second level. There one can found fitness centre, rehabilitation and relax centre, indoor golf, badminton, climbing wall, space for aerobic or zumba on the next floors. There is a restaurant offering the unique view on the town on the top of the building. The roof of the basketball hall is green with the minigolf. The essential component of the design is façade with blinds which enables the dynamically changing appearance of the building.

Purpose: Monitor changes in force production, running economy (RE), and running kinematics (RK) in a highly trained marathon runner after beginning a strength training (ST) program. Methods: One marathon runner (M, 27 y, 165 cm, 53.3 kg, VO2pesk 67.43 ml/kg/min, PR 2:33:13) with no history of ST completed a 12-week block periodized ST program. Baseline and reliability testing was conducted over a two-month period prior to ST. The completed ST RPE and work (volume load*displacement) and running volume (km/wk) were monitored over the 12 weeks. The athlete performed an isometric mid-thigh pull to assess peak force (PF, N), rate of force development at 250 ms (RFD250), and net impulse at 250 ms (NI250) during baseline and throughout ST. The athlete performed a steady state test on a treadmill instrumented with the OptojumpTM optic sensor system to assess RE (ml/kg/km) and RK during baseline and throughout ST. Impact of the ST program was assessed by percent change of the variables during the taper from the baseline average and by the odds of a true change using the typical error and smallest worthwhile change. Results: PF improved (120:1 odds) by 17.11%, RFD250 improved (22:1 odds) by 24.73%, and NI250 improved (10:1 odds) by 16.70% before competition. Ground contact time decreased (7:1 odds) by 2.57%, flight time decreased (1:1 odds) by 1.49%, step rate increased (2:1 odds) by 2.28%, and step length decreased (57:1: odds) by 2.21%. RE improved (3:1 odds) by 2.09%. Conclusion: Improving a runner’s maximal strength and rate of force development may positively influence RK and RE.

The purpose of this study was to investigate the kinetic and morphological adaptations that occur during distinct phases of a block periodized training cycle in weightlifters. Athlete monitoring data from nine experienced collegiate weightlifters was used. Isometric mid-thigh pull (IMTP) and ultrasonography (US) results were compared to examine the effects of three specific phases of a training cycle leading up to a competition. During the high volume strength-endurance phase (SE) small depressions in rate of force development (RFD) but statistically significant (p ≤ 0.05) increases in vastus lateralis cross-sectional area (CSA), and body mass (BM) were observed. The lower volume higher intensity strength-power phase (SP) caused RFD to rebound above pre-training cycle values despite statistically significant reductions in CSA. Small to moderate increases only in the earlier RFD time bands (<150 >ms) occurred during the peak/taper phase (PT) while CSA and BM were maintained. Changes in IMTP RFD and CSA from US reflected the expected adaptations of block periodized training phases. Changes in early (<100 >ms) and late (≥150 ms) RFD time bands may not occur proportionally throughout different training phases. Small increases in RFD and CSA can be expected in well-trained weightlifters throughout a single block periodized training cycle.

Tyrimo objektas: Įvairaus amžiaus sportininkų 20 metrų bėgimo iš aukšto ir žemo starto rezultatas. Tyrimo tikslas: Išanalizuoti įvairaus amžiaus sportininkų 20 metrų bėgimo iš aukšto ir žemo starto rezultatus ir jų skirtumo priežastis. Tyrimo uždaviniai: 1. Nustatyti skirtingo amžiaus sportininkų rezultatų skirtumą tarp 20 m bėgimo iš aukšto ir žemo starto. 2. Nustatyti rezultatų skirtumo tarp 20 m bėgimo iš aukšto ir žemo starto priežastis vaikų amžiaus grupėje. 3. Nustatyti rezultatų skirtumo tarp 20 m bėgimo iš aukšto ir žemo starto priežastis suaugusių amžiaus grupėje. Tiriamieji: Skirtingo amžiaus sprinteriai. Hipotezė: Jaunesnio amžiaus sprinteriai parodo geresnius rezultatus bėgant 20 m iš aukšto starto nei iš žemo starto Pagrindiniai rezultatai: 9-14 metų sportininkai geresnius 20 metrų bėgimo rezultatus parodė bėgdami iš aukšto starto, jų rezultato vidurkis 3,81±0,3 (s) . 15-16 metų sportininkai parodė panašius rezultatus bėgdami tiek iš aukšto, tiek iš žemo starto, jų rezultato vidurkis 3,65±0,2 (s) . O patys vyriausi bėgikai 21-23 metų geriausius rezultatus parodė bėgdami iš starto atramėlių jų rezultatas 3,07±0,15 (s) . Testuojant 2013 metais, 9-14 metų sportininkus, jie taip pat parodė geresnius rezultatus bėgant iš aukšto starto 3,75±0,2 (s), bėgdami iš žemo starto jie parodė 3,93±0,3 (s). Aukšto meisriškumo sportininkai bėgdami iš žemo starto parodė 2,93±0,2 (s) laiką, o iš aukšto 3,15±0,3 (s). Išvados: 1. Didesnio meistriškumo (suaugusių amžiaus grupės)... [toliau žr. visą tekstą]
The object: The athletes of all ages 20 meters running from the high and low start results The main aim: Analysis of the athletes of all ages 20 meters running from standing and block start results and their reasons for the difference. Objectives of the study: 1. Determine the difference between the results of different age of athletes running 20 meters from standing and block start. 2. Determine the difference between the results of the 20 meters of running from standing and block start reason child age group. 3. Determine the difference between the results of 20 meters of running from standing and block start of the adult age group. Research: Different age sprinters. Hypothesis: For younger sprinters show improved results over 20 meters from a high of start than the low start. The main results:9-14 years-old athlete running 20 meters have shown better results fleeing from a standing start, the average of the results are 3,81±0,3 (s). 15-16 years-old athlete running 20 meters have shown similar results but fleeing from both the standing and the block starts, the average of the results are 3,65±0,2 (s). And, the oldest runners 21-23 years-old had showed the best score while running from the block acceleration, their the average of the results are 3,21±0,15 (s.). By 2013 9-14 years-old athletes results, we can see, that they also had showed better results over time from a standing start 3,75±0,2 (s), running from a block start 3,93±0,3 (s). High-skills athletes running from... [to full text]

The master thesis Sanatorium with Speleotherapy for Children - Ostrov u Macochy is an architectonical concept which consist of the establishment of a sanatorium with various functions for patients and the public. The compound offers a medical block, administrative and economic blocks, a school, a cafeteria for visitors, a guests house for adult clients and complement. The building site is located on an inclined terrain near Císařské jeskyně (Caesar cave) and it is surrounded by forest from three sides. There is a scenic view to the south from the construction site. The neighboring buildings consist only of family houses. The compound of sanatorium aimed to have the closest interaction with surroundings and the terrain, therefore the estate is divided into three different segments based on the three most important functions of the construction program.

This Master's thesis deals with design of accommodation facility. The building si situated in the town of Boskovice, close to sports complex Červená zahrada. The buiding was built on slope. It contains three above floor, one basement floor which is accsesible from the ground. On the basement floor, there is sports complex. On the first floor, there is restaurant. The second and the third floor is intended for accommodation. The building has capacity of 50 beds. These are divided into one to four-bed rooms. The bricked construction system is made of sand-lime blocks. The ceilings are from prestressed concrete floor slab. The buiding has green roof. The work includes project documentation for the construction.

The purpose of this monitoring study was to investigate how alterations in training affect changes in force-related characteristics and weightlifting performance. Subjects: Seven competitive weightlifters participated in the study. Methods: The weightlifters performed a block style periodized plan across 20 weeks. Force plate data from the isometric mid-thigh pull and static jumps with 0 kg, 11 kg, and 20 kg were collected near the end of each training block (weeks 1, 6, 10, 13, 17, and 20). Weightlifting performance was measured at weeks 0, 7, 11, and 20. Results: Very strong correlations were noted between weightlifting performances and isometric rate of force development (RFD), isometric peak force (PF), peak power (PP), and jump height (JH). Men responded in a more predictable manner than the women. During periods of higher training volume, RFD was depressed to a greater extent than PF. JH at 20 kg responded in a manner reflecting the expected fatigue response more so than JH at 0 kg and 11 kg. Conclusions: PF appears to have been more resistant to volume alterations than RFD and JH at 20 kg. RFD and JH at 20 kg appear to be superior monitoring metrics due to their “sensitivity.”

This thesis wants to save character and identity of local. So it offers comfortable and special living and brings new values for the local inhabitants. Integrate suitable functions to improve the quality of quater.

The main design philosophy is gentle extension of the existing block with emphasis on keeping the character of Stare Brno untouched and enhance the area with missing functions, which would complement and rejuvenate both completed and projected house building development.

The diploma thesis follows up the area in the center of Náchod along the former textile factory Tepna, which is located at the transition between the historical center and the housing estate. The proposal is based on problem analysis and uses the potential, limits and quailities of the area. The concept is a new urban quarter with a residential block of buildings complemented by civic amenities and high-quality public spaces. The design preserves the existing building of the Bílá Tepna, for which a new functional use is proposed. The proposal is solved with respect to the character of the place, the connection to the surrounding buildings and the emphasis on the preference of pedestrian traffic over the automobile traffic.

The theme of the work is revitalisation of the Brno rivers, specifically the river Svitava. The main idea of the work is the understanding the river as a city-building element, as a place of potential that every city should fully utilize. People like to spend their free time at the water and such a space should satisfy their needs. The river flows smoothly throught the city, and people should be able to move freely around it. In the ideal case, it should be a green path across urban area. The solved territory is situated in the Brno-Zábrdovice district, which hasn´t ideal reputation. Its image is shaped by, among others, industrial areas which are quite often derelict. My task was to correctly distribute one of these sites, which is located near the Zábrdovice Monastery. The solved territory is currently a non-coherent and inconsistent structure that does not support the image of the site. The river is completely supressed at this point and the movement around it is very limited. In my design I tried to create a place that would offer a lot of activities through the day. I have modified the functions I have in the territory and supported by my proposal to form a working entity together. In such a territory will meet people, who work or live here, who are using the services or come to sport or spend their free time in the pleasant environment of Svitava river.

The thesis deals with the design of the area after the former brownfield of Tepna Náchod. The aim of the thesis is activate the territory, to revive the city center and to design the buildings in connection with the historical center and the chateau with a suitable functional structure, responding to the city's shortcomings. The proposal respects the character of the place trying to prefer pedestrian and cycling – pedestrian zones, cycling zones. The concept is based on the maximum use of built-up areas in the designed area and on the permeability of the entire area, the interconnection of three squares and the creation of a new public space. Furthermore, the concept responds to the historical city center and the chateau hill - views, perspective axes. Newly designed buildings are residential buildings with active parterres, administrative building for research and development, multi-purpose hall, retirement home, kindergarten, federal house and parking house. The new development clearly defines the street network. Part of the design is the planting of a park under the chateau hill with the revival of the trough of the Plhovsky´s stream.

Diploma thesis The Brownfield solutions within the Czech republic - Tepna Náchod, solves the unused territory after the former textile factory in close proximity to the historical centre of the city. The thesis deals with the analysis of the current state of the territory, its problems and the proposal of the new urban structure. It focuses on working with public space, a block structure linked to the historical centre and connecting the area to its surroundings. The aim is to create a functioning urban structure in an interesting city location.

The theme of the diploma thesis "Brno waterfront - rehabilitation of the Brno rivers and their surroundings" is creating an urban proposal in Brno, Zábrdovice, on the eastern waterfront of the Svitava river. The intention is to use the potential of the area and create the possibility of lucrative housing by river with a commercial ground floor, services, leisure time park with a multifunctional playground and easy direct access to the Svitava river. The studied location, has an area of 6.32 hectares andis defined mainly by the Zábrdovická Street and the Svitava River near the former Premonstratensian Monastery, which now serves as a Military Hospital. At present, the location has mainly low-floor industrial buildings with storage and expedition functions. The proposed buildings relate to existing buildings on the east and west banks of the Svitava River. They have the form of four to six storey blocks, which are characteristic for this area. The park connects to the revitalized eastern embankment of the river at the site of a new footbridgethat connects both rive sides and stairs. They provide space formeetings and relaxation, but also serve as a flood control in the event of a water inflow. Along the Svitava River there is a sidewalk with street alleyways and access to the river.

The diploma thesis deals with the design and implementation of the system that will control the water supply for the family house and its garden. The system aslo allows you to store a history that can be viewed via the web interface. The web interface also allows you to set system parameters and monitor the current status.

Availability of multiple disease resistant genotypes is especially important in international breeding centers where hundreds of crosses are prepared annually, and the availability of parents having resistance for multiple diseases would potentially facilitate breeders task in combining multiple disease resistance in a single cross. The principal aim of this study was to identify novel sources of resistance to multiple wheat fungal pathogens including wheat head and leaf blight diseases. For this goal, wheat genotypes of different geographic origins were tested for different FHB resistance types and four leaf spotting diseases including Tan spot (TS), Stagonospora nodorum blotch (SNB), Septoria tritici blotch (STB), in addition to Spot blotch (SB), independently.

Pyrenophora teres, the causal agent of net blotch of barley (Hordeum vulgare L.), exists in two forms; P. teres f. teres and P. teres f. maculata. Both forms induce a combination of brown necrotic spots and extensive chlorosis in susceptible barley cultivars. Although a number of low molecular weight compounds (LMWCs) have been previously isolated from P. teres culture filtrates, they only induced certain components of symptoms. Fungal metabolites were extracted from culture filtrates of both forms of the pathogen and separated into low (<3kDa) and high molecular weight compounds (HMWCs, >10 kDa) with each fraction inducing a component of the net blotch symptoms in a barley leaf toxicity assay. Inactivation of both LMWCs (<1kDa) and HMWCs resulted in loss of activity confirming their potential role in symptom development. Low molecular weight compounds induced chlorosis and water soaking but not the brown necrotic spots or lesions usually seen during the infection of barley by P. teres. The high molecular weight compounds (>10 kDa) induced the brown necrotic spots or lesions with no chlorosis evident. Further characterisation of LMWCs showed that they are not host specific while HMWCs exhibited host specificity. LMWCs were purified and further analysed using high voltage paper electrophoresis, staining and mass spectrometry. Electrophoretic properties and staining of the LMWCs with ninhydrin indicated that both forms of P. teres produced similar LMWCs in the conditions grown. Each form produced eight ninhydrin-positive compounds with the samerelative mobilities. Each individual compound was shown to induce chlorosis in excised barley leaves. All compounds except the one isolated in this study appear to be derivatives of or are the previously described compounds; N-(2-amino-2carboxyethyl) aspartic acid (Toxin A), aspergillomarasmine A, anhydroaspergillomarasmine A and aspergillomarasmine B. The exception is a bioactive UV absorbing LMWC which appears to be a reductive conjugation of the α-keto acid of phenylalanine with Toxin A. The HMWCs (>10kDa) were proteinaceous since they were identifiable using Coomassie staining. Additionally, the loss of activity that occurred with incubation at 40, 60, and 80 °C for 30 and 60 min followed a pattern fairly typical for protein denaturation. Further, treatment with protease decreased their phytotoxicity in proportion to the amount of enzyme used. Enzyme and heat treatment of proteins extracted from each form showed that proteins of P. teres f. teres are more resistant to heat and enzyme treatment compared with those of P. teres f. maculata. This suggests the protein(s) involved in symptom induction by P. teres f. teres and P. teres f. maculata are different which contributes to the difference in the symptom expression during the interaction between the pathogens and barley. Proteinaceous metabolites extracted from P. teres f. teres and P. teres f. maculata ranged from 10 to 100 kDa. Fractions purified using gel filtration had biological activity when they contained eight proteins when extracted from P. teres f. maculata (90, 80, 75, 55, 48, 35, 14 and 12 kDa) and six proteins when extracted from P. teres f. teres (90, 80, 55, 48, 14 and 12 kDa). Additionally, intercellular washing fluids (IWF) extracted from barley plants inoculated with both forms of P. teres, contained proteins of the same size as those in the biologically active fractions extracted from culture filtrates of P. teres f. maculata (80, 14 and 12 kDa) and P. teres f. teres (80, 48 and 14 kDa). Automated MS/MS sequencing of the biologically active proteins showed no resemblance to the sequences or conserved domain information available in public databases and as a consequence, these proteins were considered as novel proteins for P. teres. However, exact short matches with fragments resulting from the 80, 48 and 14 kDa proteins, showed considerable homology with ATP-binding cassette (ABC) transporters and their components, cellulases, serine proteinases as well as some hypothetical proteins isolated from different fungal species. Reaction of six plant species including one susceptible barley cultivar (Sloop) and one resistant line (CI9214) to P. teres showed that partially purified proteins induce the symptoms selectively in barley cultivars where the proteinaceous metabolites only induced brown necrotic spot/lesions in barley with a greater response seen on the susceptible cultivar Sloop when compared to the resistant line CI9214. No symptoms were seen on other plant species employed in this study suggesting that the proteinaceous metabolites isolated in this study are host specific phytotoxins. This research has allowed the first isolation of proteinaceous host-specific toxins from P. teres as well as the identification of a UV-sensitive LMWC phytotoxin not previously described. Proteinaceous toxins induced brown necrotic spots/lesions specific to the host while the LMWCs induced chlorosis in a number of different plant species. This contributes significantly to the body of knowledge defining how symptoms are caused during the pathogenicity process in the interaction between P. teres and barley.
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Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2007

Pyrenophora teres, the causal agent of net blotch of barley (Hordeum vulgare L.), exists in two forms; P. teres f. teres and P. teres f. maculata. Both forms induce a combination of brown necrotic spots and extensive chlorosis in susceptible barley cultivars. Although a number of low molecular weight compounds (LMWCs) have been previously isolated from P. teres culture filtrates, they only induced certain components of symptoms. Fungal metabolites were extracted from culture filtrates of both forms of the pathogen and separated into low (<3kDa) and high molecular weight compounds (HMWCs, >10 kDa) with each fraction inducing a component of the net blotch symptoms in a barley leaf toxicity assay. Inactivation of both LMWCs (<1kDa) and HMWCs resulted in loss of activity confirming their potential role in symptom development. Low molecular weight compounds induced chlorosis and water soaking but not the brown necrotic spots or lesions usually seen during the infection of barley by P. teres. The high molecular weight compounds (>10 kDa) induced the brown necrotic spots or lesions with no chlorosis evident. Further characterisation of LMWCs showed that they are not host specific while HMWCs exhibited host specificity. LMWCs were purified and further analysed using high voltage paper electrophoresis, staining and mass spectrometry. Electrophoretic properties and staining of the LMWCs with ninhydrin indicated that both forms of P. teres produced similar LMWCs in the conditions grown. Each form produced eight ninhydrin-positive compounds with the samerelative mobilities. Each individual compound was shown to induce chlorosis in excised barley leaves. All compounds except the one isolated in this study appear to be derivatives of or are the previously described compounds; N-(2-amino-2carboxyethyl) aspartic acid (Toxin A), aspergillomarasmine A, anhydroaspergillomarasmine A and aspergillomarasmine B. The exception is a bioactive UV absorbing LMWC which appears to be a reductive conjugation of the α-keto acid of phenylalanine with Toxin A. The HMWCs (>10kDa) were proteinaceous since they were identifiable using Coomassie staining. Additionally, the loss of activity that occurred with incubation at 40, 60, and 80 °C for 30 and 60 min followed a pattern fairly typical for protein denaturation. Further, treatment with protease decreased their phytotoxicity in proportion to the amount of enzyme used. Enzyme and heat treatment of proteins extracted from each form showed that proteins of P. teres f. teres are more resistant to heat and enzyme treatment compared with those of P. teres f. maculata. This suggests the protein(s) involved in symptom induction by P. teres f. teres and P. teres f. maculata are different which contributes to the difference in the symptom expression during the interaction between the pathogens and barley. Proteinaceous metabolites extracted from P. teres f. teres and P. teres f. maculata ranged from 10 to 100 kDa. Fractions purified using gel filtration had biological activity when they contained eight proteins when extracted from P. teres f. maculata (90, 80, 75, 55, 48, 35, 14 and 12 kDa) and six proteins when extracted from P. teres f. teres (90, 80, 55, 48, 14 and 12 kDa). Additionally, intercellular washing fluids (IWF) extracted from barley plants inoculated with both forms of P. teres, contained proteins of the same size as those in the biologically active fractions extracted from culture filtrates of P. teres f. maculata (80, 14 and 12 kDa) and P. teres f. teres (80, 48 and 14 kDa). Automated MS/MS sequencing of the biologically active proteins showed no resemblance to the sequences or conserved domain information available in public databases and as a consequence, these proteins were considered as novel proteins for P. teres. However, exact short matches with fragments resulting from the 80, 48 and 14 kDa proteins, showed considerable homology with ATP-binding cassette (ABC) transporters and their components, cellulases, serine proteinases as well as some hypothetical proteins isolated from different fungal species. Reaction of six plant species including one susceptible barley cultivar (Sloop) and one resistant line (CI9214) to P. teres showed that partially purified proteins induce the symptoms selectively in barley cultivars where the proteinaceous metabolites only induced brown necrotic spot/lesions in barley with a greater response seen on the susceptible cultivar Sloop when compared to the resistant line CI9214. No symptoms were seen on other plant species employed in this study suggesting that the proteinaceous metabolites isolated in this study are host specific phytotoxins. This research has allowed the first isolation of proteinaceous host-specific toxins from P. teres as well as the identification of a UV-sensitive LMWC phytotoxin not previously described. Proteinaceous toxins induced brown necrotic spots/lesions specific to the host while the LMWCs induced chlorosis in a number of different plant species. This contributes significantly to the body of knowledge defining how symptoms are caused during the pathogenicity process in the interaction between P. teres and barley.
Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2007

Collective case study (Creswell, 2014; Stake, 1995) was used to explore the journey of coach-athlete dyads who were able to successfully maintain their training and interpersonal relationships throughout the course of the athlete enduring a mental block. Three coach-athlete dyads, plus one additional athlete, completed in-depth one-on-one interviews, discussing their coach-athlete relationship before, during, and after the mental block. All dyads were same sex, nationally ranked coach-athlete pairs, from sports involving mandatory elements that include both twisting and flipping components. Categorical aggregation of participant statements lead to the formation of five main themes associated with dyads successfully overcoming a mental block (where success was defined as the athlete regaining the ability to perform the skill that they had previously been unable to do on account of the mental block and the dyad maintaining their training and interpersonal relationship): 1) Get to Know Your Athlete: The Need for High Quality Communication; 2) Be a United Front; 3) Mistakes and Miscommunications Happen: Recovery is Key; 4) Seek Outside Resources; 5) Be Patient. Results suggest that an environment for success can flourish when each party is open, honest, and self-aware of their own limitations. It is suggested that future research utilize the 3 + 1Cs Model of the coach-athlete relationship in exploring how dyads can successfully overcome a mental block.

O objetivo do presente relatório consiste em analisar se a implementação do Programa de Apoio à Educação Física Curricular – Bloco de Natação (P.A.E.F.C. – Bloco de Natação) resultou numa alteração da realidade desportiva do Município de Lisboa e em que medida essa alteração foi positiva. Particularmente procura saber a importância, influência e impacto que o Programa tem vindo a apresentar nos alunos das escolas do 1º Ciclo do Ensino Básico e nas piscinas que colaboram com o Bloco de Natação. O estudo apresentado no relatório tem três pontos fundamentais: centrados no impacto do Bloco de Natação no Programa Clubes do Mar e na Piscina de São Vicente (Alfama), ambos no ano letivo 2017/2018; nas opiniões dos Diretores Técnicos das Piscinas que colaboram com Bloco de Natação; e na análise da Tendência que o Programa tem vindo a apresentar desde a sua implementação até à atualidade. A realização do estudo passou por diversas fases de desenvolvimento, em primeiro a recolha de dados e posteriormente a análise dos mesmos, sendo possível por último reunir e concluir a análise de toda a informação com todos os pontos fundamentais do estudo, em conjunto. Desta forma, perante os dados analisados é possível verificar que o P.A.E.F.C. – Bloco de Natação tem vindo a aumentar a sua influência junto do município de Lisboa, pois tem crescido em diversos aspetos ao longo dos anos desde a sua implementação. O Bloco de Natação tem evoluído de forma positiva no sentido dos seus objetivos previamente definidos que visam proporcionar as condições básicas e fundamentais no que diz respeito à adaptação ao meio aquático e à aprendizagem da natação, que são consideradas competências basilares no processo formativo das crianças que se encontram definidas no programa curricular oficial da Expressão e Educação Física Motora, e também de forma geral no sentido do valor social e de forma particular na oferta de educação aos alunos através dos valores desportivos. Assim sendo, é possível afirmar que a implementação do Bloco de Natação conduziu a uma alteração da realidade do Município de Lisboa no âmbito do desporto de uma forma positiva, como comprovam os dados de crescimento do Programa, desde a sua implementação. Conclui-se assim que a Câmara Municipal de Lisboa, através da implementação do P.A.E.F.C. – Bloco de Natação apresenta uma alteração da realidade desportiva do Município de forma positiva, esta tem criado condições por via do desporto para o desenvolvimento sustentável da sua cidade, indo assim ao encontro do paradigma das cidades Europeias da atualidade, que se Página | 4 definem como “cidades sustentáveis” e mais recentemente como “cidades inteligentes”. Pois, de acordo com a (United Nations Office on Sport for Development and Peace, 2014) na “2030 Agenda for Sustainable Development”, “o desporto é encarado como um grande contributo para a concretização o desenvolvimento sustentável e da paz é promovendo a tolerância, respeito, poder associado a mulheres e jovens, comunidades, assim como contribuições para serem efetivados os objetivos delineados para a saúde, educação e inclusão social”.
The objective of this report is to examine whether the implementation of the Program to Support the Physical Education Curriculum – Swimming Block (P.A.E.F.C. - Swimming Block) resulted in a change of the reality of the sports city of Lisbon and to what extent this change was positive. In particular, it seeks to know the importance, influence and impact the program has had on the elementary school students (1st to 4th grade) that collaborate with the Swimming Block. The study presented in this report has three fundamental points: focusing on the impact of swimming at the Sea Club Program, and in the São Vicente (Alfama) pool, both in the academic year 2017/2018; in reviews of the Technical Directors of the pools that collaborate with the Swimming Block; and in the analysis of the trend that the program has been present since its implementation to the present. The completion of the study went through various stages of development, particularly in the first data collection and analysis, allowing for the compilation and conclusion of the analysis of all the information with its fundamental key points, as a whole. Confronting the data, it is possible to conclude that the P.A.E.F.C. – Swimming Block has been increasing its influence in the county of Lisbon due to the fact that it has grown in many aspects over the years since its implementation. The Swimming Block has positively grown when in regards to its previously defined objectives in the aims of providing the basic and fundamental conditions regarding the adaptation to the aquatic environment and swimming education, which are considered core skills in the formative process of children under the “Expressão e Educação Física Motora” official curricular programme. It has also generally evolved with respect to its social value, particularly through providing education to students by sports figures. Therefore, it is possible to confirm that the implementation of the Swimming Block has led to a positive change in the county of Lisbon in regards to the reality of sports, as the programme’s data concludes, ever since it was implemented. In conclusion, through the implementation of the P.A.E.F.C. – Swimming Block, the Municipal Council of Lisbon has grown positively within its sports panorama, by using sports as a method of contributing to the sustainable development of the city, putting it on par with the panorama Página | 6 of the modern European cities, self-defined as “sustainable cities” or most recently as “inteligente cities”. According to the (United Nations Office on Sport for Development and Peace, 2014) in “2030 Agenda for Sustainable Development”: Sports are seen as a great contributer to sustainable development and peace, through the promotion of tolerance, respect, empowerment of women and the youth, community, as well as health, education and social inclusion.