Dissertations / Theses on the topic 'Sporulation'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Sporulation.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
com, rohanlowe@gmail, and Rohan George Thomas Lowe. "Sporulation of Stagonospra nodorum." Murdoch University, 2006. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20071101.221432.
Full textLowe, Rohan George Thomas. "Sporulation of Stagonospora nodorum /." Access via Murdoch University Digital Theses Project, 2006. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20071101.221432.
Full textLowe, Rohan George Thomas. "Sporulation of Stagonospra nodorum." Thesis, Lowe, Rohan George Thomas (2006) Sporulation of Stagonospra nodorum. PhD thesis, Murdoch University, 2006. https://researchrepository.murdoch.edu.au/id/eprint/166/.
Full textLowe, Rohan George Thomas. "Sporulation of Stagonospra nodorum." Lowe, Rohan George Thomas (2006) Sporulation of Stagonospra nodorum. PhD thesis, Murdoch University, 2006. http://researchrepository.murdoch.edu.au/166/.
Full textUnderwood, Sarah. "Sporulation initiation in Clostridium difficile." Thesis, University of Leeds, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.505066.
Full textCardaman, Richard C. "Sporulation mutants of Myxococcus xanthus." Case Western Reserve University School of Graduate Studies / OhioLINK, 1994. http://rave.ohiolink.edu/etdc/view?acc_num=case1057779064.
Full textPolak, Eline. "Asexual sporulation in the basidiomycete Coprinus cinereus /." [S.l.] : [s.n.], 1999. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=13125.
Full textDavidson, Philip. "Evolutionary Remodeling of the Sporulation Initiation Pathway." Research Showcase @ CMU, 2017. http://repository.cmu.edu/dissertations/1026.
Full textAnco, Daniel J. "Epidemiological Studies of the Sporulation Potential and Environmental Factors Affecting Sporulation of Phomopsis viticola on Infected Grapevines." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1322495236.
Full textArab, Najafi Seyed Mahmoud. "Control of compartment-specific sigma (#sigma'F) in Bacillus subtilis." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318858.
Full textKarandikar, Atul. "Streptomyces coelicolor A3(2) : growth and differentiation of surface grown cultures." Thesis, Liverpool John Moores University, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242153.
Full textBone, E. J. "Biochemistry and genetics of sporulation in Bacillus subtilis." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377250.
Full textMerrill, C. "Radiation-induced genetic change during sporulation in Saccharomyces cerevisiae." Thesis, Swansea University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.638182.
Full textRyding, Nicholas Jamie. "Analysis of sporulation genes in Streptomyces coelicolor A3(2)." Thesis, University of East Anglia, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296928.
Full textRoberts, David Michael. "Chromosome organisation and segregation during sporulation in Bacillus subtilis." Thesis, University of Newcastle upon Tyne, 2018. http://hdl.handle.net/10443/4080.
Full textHall, Holly. "The sporulation-specific small regulatory RNAs of Bacillus subtilis." Thesis, University of Warwick, 2017. http://wrap.warwick.ac.uk/102346/.
Full textMeador-Parton, Jennifer L. "Structural Analysis of Bacillus subtilis Spore Peptidoglycan During Sporulation." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/30922.
Full textMaster of Science
Würfl, Stephanie. "Molekularbiologische Untersuchungen zur Regulation der Sporulation in Clostridium acetobutylicum." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-65434.
Full textDembek, Marcin. "Whole-genome analysis of sporulation and germination in Clostridium difficile." Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/38630.
Full textReodica, Mayfebelle Biotechnology & Biomolecular Sciences Faculty of Science UNSW. "Transcriptional repression mechanisms of sporulation-specific genes in saccharomyces cerevisiae." Awarded by:University of New South Wales. School of Biotechnology and Biomolecular Sciences, 2006. http://handle.unsw.edu.au/1959.4/32731.
Full textStevens, Christine. "The regulation of gene expression during sporulation in Bacillus subtilis." Thesis, University of Oxford, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292334.
Full textShu, J. C. "Mutational studies of the regulation of sporulation in Bacillus subtilis." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288524.
Full textChapman, J. W. "A study of Bacillus subtilis sporulation genes cloned on plasmids." Thesis, Open University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.484403.
Full textChatwin, Heather M. "Molecular genetic analysis of chemical-induced sporulation of Myxococcus xanthus." Thesis, University of Warwick, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387398.
Full textRamage, Anne Donaldson. "Genetic analysis of mutations affecting the initiation of yeast sporulation." Thesis, University of Edinburgh, 1990. http://hdl.handle.net/1842/16922.
Full textHarry, Kathryn Helene. "Sporulation and enterotoxin regulation by sigma factors in Clostridium perfringens." Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/42517.
Full textMaster of Science
Palframan, Wendy Jane. "The whiD locus of Streptomyces coelicolorA3(2)." Thesis, University of East Anglia, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267722.
Full textHyde, K. D. "Spore settlement and attachment in marine fungi." Thesis, University of Portsmouth, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355131.
Full textWilson, Giles. "A study of the morphological and biochemical differentiation of Saccharopolyspora erythraea." Thesis, University of Surrey, 1994. http://epubs.surrey.ac.uk/844505/.
Full textTiley, Anna Mystica Mendez. "Investigating asexual sporulation in Zymoseptoria tritici, a fungal pathogen of wheat." Thesis, University of Bristol, 2016. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715767.
Full textPartridge, Sally Rachel. "Regulation of prespore-specific gene expression during sporulation in Bacillus subtilis." Thesis, University of Oxford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306742.
Full textIlling, Nicola. "The control of differential gene expression during sporulation in Bacillus subtilis." Thesis, University of Oxford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.276812.
Full textSaujet, Laure. "Etude du réseau de régulation de la sporulation chez Clostridium difficile." Paris 7, 2013. http://www.theses.fr/2013PA077269.
Full textClostridium difficile is a Gram-positive anaerobic spore-forming bacteria which is responsible for post-antibiotic diarrhea. Few data exist on cellular processes induced at the onset of stationary phase concomitantly with the synthesis of toxins and regulatory networks controlling stationary phase and sporulation. We constructed a sigH mutant to determine the role of SigH, which is an alternative sigma factor involved in the transcription of phase transition and initiation of sporulation genes in B. Subtilis. We compared the expression profiles of 630Aerm strain and the sigH mutant after 10 h of growth. In C. Difficile, SigH regulates the expression of many genes involved in motility, sporulation, cell division, virulence and metabolism. Finally, the expression of toxin genes is negatively regulated by SigH. We then studied the regulatory cascade of sporulation in C. Difficile, involving four sigma factors in B. Subtilis: SigF, SigE, SigG and SigK. We compared the expression profiles of the sigF, sigE, sigG and sigK mutants and the 6300erm strain. We defined the regulons of these sigma factors and showed that in C. Difficile, the SigE regulon is not under the strict dependence of SigF and SigG is not necessary for the SigK activity. We also analyzed the regulatory network in each compartment of the spore and studied the rote of SpoIIID and SpoVT regulators. This work showed a less strict communication between the two compartments of the spore in C. Difficile compared to B. Subtilis
McCue, Lee Ann. "Molecular analysis of a gene required for sporulation of Streptomyces griseus /." The Ohio State University, 1994. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487853913101721.
Full textFimlaid, Kelly Ann. "Identification Of Regulatory Factors That Control Clostridium Difficile Sporulation and Germination." ScholarWorks @ UVM, 2016. http://scholarworks.uvm.edu/graddis/458.
Full textDi, Donato Francis Anthony. "Determinants of compartmentalization of gene expression during sporulation in Bacillus subtilis." Diss., Temple University Libraries, 2008. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/4102.
Full textPh.D.;
Bacillus subtilis, a benign gram-positive bacterium, utilizes the strategy of sporulation, which enables it to survive stresses such as starvation, desiccation, and UV irradiation. The spore provides greatly heightened safety to heat and noxious chemicals and remains dormant until conditions become favorable to growth. Sporulation by Bacillus subtilis is a primitive example of cell differentiation. The study of sporulation by Bacillus subtilis has become a paradigm for the study of differentiation in prokaryotes. Central to this process is the establishment of distinct patterns of gene expression in the cell types involved. Our laboratory has developed a two-part sacB/SacY probe to study the temporal and spatial compartmentalization of gene expression. It utilizes the anti-terminator protein SacY to control the transcription of reporter lacZ, (cloned downstream of the sacB gene,) which is regulated by anti-termination. Expression of sacB and SacY is regulated by a pair of promoters specific for sF (prespore specific) and sE (mother cell specific.) Both SacY and sacB must be in the same compartment of the sporulating cell in order to obtain ß-galactosidase activity. Mutagenesis of Bacillus subtilis was employed to identify determinants of compartmentalization of gene expression during sporulation. Mutants were screened for loss of compartmentalization using the two-part probe. In addition to the two-part sacB/SacY probe, a second method was developed; transposon mutagenesis was performed on strains where expression of gfp was regulated by promoters recognized by either sF or sE. Cells deficient in sporulation were isolated and evaluated by fluorescence microscopy for uncompartmentalized gfp expression. A rescue vector was developed that allowed for efficient cloning of Tn10 insertions. This plamid, pJP17, proved to be an essential tool. Mutations causing uncompartmentalized sF activity were identified in spoIIIE, spoIIIAA, spoIIIAB, spoIIIJ, spoIIE, spoIIAA, spoIID, spoIIM, kinA and ald. The spoIIIE mutation provides the most dramatic phenotype, and was the only mutation, that resulted in 100% loss of compartmentalization during stage II of sporulation. In contrast to all other mutants, the dramatic stage II loss of compartmentalized activity of sF indicates a regulatory role for SpoIIIE, which has yet to be elucidated. Taken together, these results indicate a central role for SpoIIIE in preventing activation of sF in the mother cell in addition to its DNA translocation activity.
Temple University--Theses
Rostami, Nadia. "Co-ordination of DNA replication initiation and chromosome segregation during sporulation." Thesis, University of Newcastle upon Tyne, 2015. http://hdl.handle.net/10443/2884.
Full textMaughan, Heather. "Evolution of Sporulation in Bacillus Subtilis: Functional Loss and Evolutionary Consequences." Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/193989.
Full textGarcía, García Tránsito. "Functional analysis of phosphorylation of the replication controller YabA in Bacillus subtilis." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS506/document.
Full textUpon environmental or nutritional changes, bacteria must adjust their cell cycle with their growth rate. Most particularly, DNA replication initiation events must be controlled and coordinated with cell physiology to ensure faithful chromosome inheritance. In Bacillus subtilis, a model of Gram-positive bacteria, YabA plays a major role in down regulating initiation replication through interaction with the initiator protein DnaA and the clamp polymerase DnaN. However, YabA is a structural hub protein able to interact with other protein partners, indicating it might be multifunctional. Through its unique overall tri-dimentional structure composed of N-terminal four helix-bundle tetramer connected to four monomeric C-terminal domains by a highly flexible linker, YabA is capable to physically interact with more than one protein at a time, thus providing a suitable platform to integrate intracellular signals to replication initiation. Phosphorylation is the most prevalent post translational modification that modulates protein activities in response to cellular signals. Using in vitro phosphorylation and mass spectrometry we demonstrated that YabA is phosphorylated by the Hanks-type serine/threonine kinase YabT at a threonine residue localized within the flexible inter-domain region. YabT is a kinase activated by DNA and up-regulated during glucose starvation, sporulation and stationary phase. We constructed YabA phosphomimetic (yab-AT71D) and non-phosohorylatable (yabA-T71A) mutants to (i) confirm the requirement of T71 for YabT-mediated phosphorylation in vitro and (ii) perform in vivo and in vitro functional studies.We show in vivo that the phosphorylation of YabA is not involved in initiation control, but rather modulates bacillus developmental processes. We found that YabA phosphorylation inversely regulates sporulation and biofilm formation highlighting the multifunctional role of YabA as well as its role in integrating physiological signals to connect chromosomal replication initiation control with cell development. Our results support a role of YabT-mediated phosphorylation of YabA in Bacillus subtilis life-style decision making through the modulation of Spo0A-P intracellular levels. We established that YabA phosphorylation correlates with high cellular levels of Spo0A-P, leading to sporulation stimulation and preventing biofilm formation. Additionally, thin layer chromatography (TLC) analysis and In-Gel assays showed that YabA possess an atypical "ATP / GTPase" activity. This unusual activity seems to be modulated by phosphorylation of the YabA T71 residue. Our functional analysis pointed to a potential role of YabA in the c-di-GMP signaling transduction pathway, known to regulate biofilm formation in many bacteria. This suggesting a complex regulatory role of YabA during development, involving signaling crosstalk. LC-MS analyzes showed that when overexpressed in Escherichia coli, YabA is phosphorylated on the residue Y90 in a YabT independent manner. Y90 belongs to the interaction C-terminal domain, which contacts DnaA and DnaN. We found that Y90 was involved YabA-mediated replication initiation control. We provided evidence that phosphorylation state of YabA at Y90 can potentially modulates a protein-interaction switch with its protein partners DnaA and DnaN in a yeast-two-hybrid-based assay. Although we did not identified a kinase responsible for the phosphorylation of YabA at Y90 in B. subtilis, this finding hint at the possibility of a YabA-mediated control of initiation modulated by phosphorylation in this bacteria. Thus, all of these in vitro and in vivo observations suggest the existence of different modes of regulation of YabA activity by phosphorylation, involving threonine and tyrosine residues. This study established that YabA, apart from its role during replication initiation, plays a key regulatory role in B. subtilis development
Pozzi, Adrien C. "Rôles adaptatifs et contraintes de la sporulation chez les microorganismes associés aux plantes : cas de la sporulation in planta dans la symbiose actinorhizienne Frankia (Frankiaceae)–Alnus (Betulaceae)." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10359/document.
Full textFrankia sp. is a telluric actinobacteria able to establish a root symbiosis with actinorhizal plant such as Alnus sp. Only some Frankia strains are able to sporulate in-planta, as spores can be present in (Sp+) or absent from (Sp–) the vegetal cells of the root nodule. It is to our knowledge a unique case of endophytic sporulation. However, the description and the ecological interpretation of this original life-history trait (LHT) were scarce. Our contribution to the study of the in-planta sporulation of Alnus-infective Frankia sp. combines theoretical, descriptive and experimental approaches to precise (i) the relative effect of the bacterial strain, the host-plant species and the pedoclimatic conditions on this LHT, (ii) the effect of the of the environmental variability on the distribution, diversity and selection of the trait, and (iii) the associated costs and benefits for the two symbiotic partners. We demonstrated for the first time that the in-planta sporulation is a LHT (i) specific to some Frankia lineages, (ii) major to understand their evolutionary history and (iii) significantly correlated to particular genetic features. We also shown that the occurrence of the trait varies according to the environment We also proposed a model of the evolution of the trait taking its fitness into account. We bring all the previous considerations and results to discuss the inplanta sporulation trait within a continuum of symbiotic strategies and more generally to discuss the evolutionary ecology of plant-microbe symbioses
Gilmore, Meghan Elizabeth. "Analysis of the Roles of the cwlD Operon Products during Sporulation in Bacillus subtilis." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/35779.
Full textMaster of Science
Malleck, Tiffany. "Mécanismes d’adaptation de Moorella thermoacetica/thermoautotrophica sur les lignes de production de produits alimentaires appertisés." Thesis, Avignon, 2017. http://www.theses.fr/2017AVIG0339/document.
Full textMoorella thermoactica is a spoilage anaerobic and thermophilic spore-former producing the most highly heat-resistant spores isolated so far in food industry, which enables the bacteria to survive the sterilization process applied in cannery. M. thermoacetica is the main cause of low acid canned food spoilage after incubation at 55 °C. Little is known about sporulation mechanisms and spore properties according to environmental conditions.In this work, we aimed at characterizing the impact of environmental conditions on sporulation and spore resistance properties, as well as describing the molecular mechanisms underlying sporulation. We showed that sporulation capacities are higher when sporulation is performed at the optimal temperature (55 °C) that at limit temperatures (45 °C and 65 °C). Besides, spores are less resistant to wet heat and biocides when formed at 45 °C than at 55 °C. We showed that the ultrastructure and spore protein composition varied according to sporulation temperature. Moreover, the study of gene expression by RNAseq during sporulation in optimal regulated conditions showed that most of the 167 genes involved in the sporulation process and identified in silico in M. thermoacetica ATCC 39073 genome, were up-regulated during sporulation, suggeting that the mechanisms described in other endospore-formers are conserved in Moorella.Altogether, our results showed that sporulation temperature strongly impacts sporulation and spore properties of M. thermoacetica and that sporulation mechanisms tend to be conserved in Moorella considering data available on other endospore-formers
Sonoda, Yo. "Structural and functional analysis of a sporulation protein Spo0M from Bacillus subtilis." Kyoto University, 2016. http://hdl.handle.net/2433/215587.
Full text0048
新制・課程博士
博士(農学)
甲第19761号
農博第2157号
新制||農||1039(附属図書館)
学位論文||H28||N4977(農学部図書室)
32797
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 三上 文三, 教授 加納 健司, 教授 喜多 恵子
学位規則第4条第1項該当
Gauvry, Emilie. "Modélisation de la sporulation de Bacillus subtillis BSB1 et liens physiologiques avec les cinétiques de croissance." Thesis, Brest, 2017. http://www.theses.fr/2017BRES0140/document.
Full textSpore-forming bacteria cause health risks and alteration of food products. They can sporulate to form cells resistant to various physical or chemical aggressions. In order to limit the formation of spores in food and on production lines in the agri-food industry, a preventive approach consists in predicting this bacterial process according to the environmental conditions encountered during the manufacturing processes. For this, a kinetic model describing both growth and sporulation of the bacterium model Bacillus subtilis BSB1 was developed. This model is a useful tool for assessing the impact of environmental factors on quantitative and physiological aspects of growth and sporulation of B. subtilis. Unfavorable conditions of temperature, pH and water activity cause a slowing of B. subtilis’ growth, a more synchronous sporulation in the bacterial population leading to later spore emergence and lower spore production. All these effects have been described with a predictive model of growth and sporulation: the cardinal model. These (kinetic and cardinal) models are efficient to predict growth and sporulation of B. subtilis BSB1 in different culture conditions, different matrices and in dynamic conditions of environmental factors. This work and these mathematical models will allow a better understanding of the sporulation behavior of bacteria according to environmental factors and thus a better understanding of the sporulation in the agrofood industry
Abbas, Amina Aicha. "Effet de l’absence d’oxygène sur la capacité de sporulation et les propriétés des spores de Bacillus cereus." Thesis, Avignon, 2014. http://www.theses.fr/2014AVIG0330/document.
Full textThe effect of temperature and nutrient composition of the medium on B. cereus spore properties (resistance and germination) has been extensively studied unlike to the effect of anaerobiosis. Nevertheless, B. cereus vegetative cells can be found in a large variety of natural environments with low oxygen level (intestine, soil, food processing line) where sporulation take place. Spores produced in these anaerobic environments could have particular properties. In this work, a panel of B. cereus strains belonging to phylogenetic groups II to VII was studied for their capacity to sporulate in anaerobiosis in an appropriate sporulation medium we developed (MODS). In anaerobiosis, sporulation ability was lower and more heterogeneous than in aerobiosis. The B. cereus AH187 strain produced the highest level of spores in anaerobiosis, it was therefore chosen to study spore properties. Spores produced in anaerobiosis were more resistant to wet heat from 90°C to 100 °C, 1M NaOH, 1M nitrous acid and pulsed light. No difference in resistance to 5 % hydrogen peroxide or 0.25 mM formaldehyde or UV-C was observed between these two conditions. In the presence of L-alanine, spores produced in anaerobiosis germinated more efficiently than spore produced in aerobiosis. No difference in germination was observed with inosine. No difference in the spores size produced in the two conditions was observed by transmission electron microscopy. However, spores obtained under anaerobic conditions had a damaged exosporium, or in some cases a completely detached exosporium, unlike spores produced under aerobic conditions. To understand differences in sporulation ability between both conditions, Real-time reverse transcription-PCR was used to study the expression the expression of sporulation initiation genes spo0A, spo0B, spo0F, kinA and kinB. The kinetics of gene expression spo0A, spo0B, spo0F and kinA had the same trend. They were characterized by a higher expression in anaerobiosis compared to aerobiosis at the beginning and the end of exponential growth phase. Furthermore, kinB gene expression was characterized by an increase in anaerobiosis compared to aerobiosis to achieve a peak between 4 (middle exponential phase) and 6 (early stationary phase) hours of growth. The spo0A, spo0B, spo0F, kinA and kinB genes are differentially expressed between aerobiosis and anaerobiosis. These data may help to understand the difference in B. cereus sporulation capacity between aerobic and anaerobic condition
Tchervenivanova, Eli. "Development of a model to predict sporulation of Bremia lactucae in lettuce." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23943.
Full textElgadi, Suliman Ali. "Sporulation septation in Streptomyces as a model to investigate bacterial cell division." Thesis, Swansea University, 2012. https://cronfa.swan.ac.uk/Record/cronfa43201.
Full textDean, Amanda Marie. "Requirements for Compartmentalization of Penicillin-Binding Proteins during Sporulation in Bacillus subtilis." Thesis, Virginia Tech, 2002. http://hdl.handle.net/10919/36489.
Full textMaster of Science
Babcock, Martin J. "Characterization and regulation of a gene involved in sporulation of Streptomyces griseus /." The Ohio State University, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487676261012572.
Full textRerngsamran, Panan. "Functional analysis of fluffy, a transcriptional regulator for conidial development in Neurospora crassa." Diss., Texas A&M University, 2005. http://hdl.handle.net/1969.1/2452.
Full text