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Academic literature on the topic 'Spodoptera Litura Nuclear Polyhedrosis Virus'

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Published: 6 September 2023

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Journal articles on the topic "Spodoptera Litura Nuclear Polyhedrosis Virus"

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Syahroni, M. Novel Ghufron, and Nanang Tri Haryadi. "Uji Efektivitas Konsentrasi Spodoptera litura – Nuclear Polyhedrosis Virus (SlNPV) JTM 97C Formulasi Bubuk Terhadap Larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Pada Tanaman Kedelai." Jurnal Pengendalian Hayati 2, no. 2 (September 25, 2019): 46. http://dx.doi.org/10.19184/jph.v2i2.17140.

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Spodoptera litura (armyworm) is an important leaf pest that is polyphagic and has a broad range of hosts. Spodoptera litura is the main pest in soybean plants and can cause up to 50% damage and often results in decreased productivity and even crop failure. Spodoptera litura is widespread in areas with hot and humid climates from the subtropics to the tropics. S. litura pests attack cultivation plants in the vegetative and generative phases. The prospect of insect pathogens as a substitute for chemical insecticides is quite good in terms of their effectiveness and impact on the environment. Nuclear Polyhedrosis Virus (NPV) is one of the Baculoviridae viruses that infect the larval stage. Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) is a pathogenic virus that infects armyworms. SlNPV JTM 97C is an effective isolate in controlling larvae of Spodoptera litura. The larvae used in this study were larval instar 4, instar 5, and instar 6. The study was conducted using a Completely Randomized Design (CRD) with 6 treatments and 3 replications. The treatments consisted of controls, 1 g / l, 2g / l, 3 g / l, 4 g / l, and 5 g / l. Each treatment was applied to instar 4, instar 5 and 6 instar larvae. The results showed that SlNPV JTM 97C affected mortality, stopped eating and pupa formation of instar 4, instar 5 larvae, but did not affect instar 6 larvae. The younger the larval instar the higher the pathogenicity response.
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Hasanah, Uswatun, and Nanang Tri Haryadi. "EFEKTIVITAS BAHAN PELINDUNG Spodoptera litura NUCLEAR POLYHEDROSIS VIRUS (SlNPV) UNTUK MENGENDALIKAN Spodoptera litura (F.) PADA TANAMAN KEDELAI." Jurnal Hama dan Penyakit Tumbuhan 10, no. 3 (September 16, 2022): 148–55. http://dx.doi.org/10.21776/ub.jurnalhpt.2022.010.3.5.

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Spodoptera litura dapat menyebabkan kehilangan hasil hingga 80% bahkan gagal panen. Nuclear Polyhedrosis Virus merupakan agensi hayati dari kelompok patogen serangga famili Baculoviridae yang banyak digunakan sebagai agens hayati dan Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) merupakan salah satu pemanfaatan NPV yang efektif dalam mengendalikan Spodoptera litura. Kendala penggunaan SlNPV yaitu tidak tahan terhadap sinar matahari sehingga perlu bahan pelindung agar virus tidak mengalami inaktif. Penelitian ini bertujuan untuk mengetahui pengaruh bahan pelindung dalam mempertahankan efektivitas SlNPV dari paparan sinar matahari. Penelitian dilakukan di rumah kaca dengan menggunakan Rancangan Acak Lengkap (RAL) non faktorial. Perlakuan meliputi SlNPV 100 ml tanpa pemaparan UV A (a), SlNPV 100 ml tanpa bahan pelindung + UV A (b), SlNPV 100 ml + Molase 40% + UV A (c), SlNPV 100 ml + Kaolin 40% + UV A (d), SlNPV 100 ml + Tinopal 1% + UV A (e), SlNPV 100 ml + Sunblock SPF 50 5% + UV A (f). Setiap perlakuan diulang sebanyak 4 kali sehingga didapatkan 24 satuan percobaan. Hasil penelitian menunjukkan bahwa pada uji skala rumah kaca perlakuan SlNPV + kaolin 40% dapat menyebabkan mortalitas S. litura 92,5% diikuti oleh perlakuan SlNPV + molase 40% dengan mortalitas sebesar 75% pada pengamatan 9 hari setelah aplikasi. Kaolin efektif digunakan sebagai bahan pelindung SlNPV dibandingkan bahan pelindung lainnya karena dapat melindungi SlNPV dari paparan sinar Ultra Violet (UV) karena membentuk lapisan seperti lapisan film.
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Monobrullah, M. D., and Masao Nagata. "EFFECTS OF LARVAL AGE ON SUSCEPTIBILITY OF SPODOPTERA LITURA (LEPIDOPTERA: NOCTUIDAE) TO SPODOPTERA LITURA MULTIPLE NUCLEAR POLYHEDROSIS VIRUS." Canadian Entomologist 132, no. 3 (June 2000): 337–40. http://dx.doi.org/10.4039/ent132337-3.

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Susceptibility of many insects to baculoviruses decreases with host development (Hochberg 1991), whereas the time required for infected hosts to die increases with larval age (Boucias et al. 1980; Bucher and Turnock 1983; Smits and Vlak 1988). The actual mechanism of this phenomenon, however, has not been established in many cases. The present study was undertaken to investigate the effect of larval age on the susceptibility of Spodoptera litura Fabricius to nuclear polyhedrosis virus (NPV) through oral and intrahaemocoelic routes of infection.
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Afidah, Nafisatul, Tutung Hadiastono, Bedjo Bedjo, and Fery Abdul Choliq. "Compatibility of Lemongrass Extract with Spodoptera litura Nuclear Polyhedrosis Virus Against Spodoptera litura on Soybean Plants." Journal of Tropical Plant Protection 2, no. 1 (January 1, 2021): 7–13. http://dx.doi.org/10.21776/ub.jtpp.2021.002.1.2.

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Armyworm (Spodoptera litura) is an important pest that damages the leaves of soybean. SlNPV is one type of pathogen that has potential as a biocontrol agent to control armyworms because it is specific, selective, and effective. This study aimed to investigate the compatibility between the lemongrass and SlNPV in controlling armyworm. The Research was conducted at the Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) from February 2016 until April 2016. The study using completely randomized design with 7 treatments and 3 replication with a treatment that consists of control, SlNPV-JTM 97C 1,5 × 106 PIB/ml, 10% of lemongrass extract, a combination SlNPV-JTM 97C fixed concentrate of 1,5 × 106 PIB/ml with 2.5%, 5%, 7.5%, and 10% of lemongrass extract. The results showed that the combination of SlNPV-JTM 97C 1.5 × 106 PIB/ml with 10% lemongrass had the highest level of virulence, with 96% of mortality on larva 100% on the pupa stage, so that the combination of SlNPV and lemongrass extracts was compatible in controlling S.litura.
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Maeda, S., Y. Mukohara, and A. Kondo. "Characteristically distinct isolates of the nuclear polyhedrosis virus from Spodoptera litura." Journal of General Virology 71, no. 11 (November 1, 1990): 2631–39. http://dx.doi.org/10.1099/0022-1317-71-11-2631.

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Das, Rakha, and Y. Durga Prasad. "Restriction endonuclease analysis of a Spodoptera litura nuclear polyhedrosis virus (NPV) isolate." IUBMB Life 39, no. 1 (May 1996): 1–11. http://dx.doi.org/10.1080/15216549600201001.

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Im, Dae Joon, B. M. Shepard, and R. M. Aguda. "Pathogenicity and Histopathology of a Nuclear Polyhedrosis Virus of Spodoptera litura (Fab.)." International Journal of Tropical Insect Science 9, no. 04 (August 1988): 539–42. http://dx.doi.org/10.1017/s1742758400011115.

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N, DHANDAPANI, and SUNDARA BABU P.C. "EFFICACY OF NUCLEAR POLYHEDROSIS VIRUS AGAINST Spodoptera litura ON GROUNDNUT IN DIFFERENT LOCATIONS OF TAMIL NADU." Madras Agricultural Journal 82, september October (1995): 531–33. http://dx.doi.org/10.29321/maj.10.a01256.

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Field trials conducted against Spodoptera litura (Fb.) with nuclear polyhedrosis virus (NPV) on groundnut (Arachis hypogaea L.) in eight different locations of Tamil Nadu revealed that two rounds of application of NPV at 250 Larval equivalent (LE)/ha with the adjuvant crude sugar at 2.5 kg/ha at ten days interval was significantly superior than untreated check in reducing the larval population and increasing the pod yield. irrespective of the locations tested.
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Miranti, Mia, Wawan Hermawan, Melanie ., Rahman Perdana Hadi, Dicky Budi Sugiarto, and Desy Efriyani Anggraeny Nasution. "THE POTENTIAL OF SUBCULTURE Helicoverpa armigera NUCLEAR POLYHEDROSIS VIRUS (HaNPV) TO BE UTILIZED AS AN ALTERNATE SYNTHETIC INSECTICIDES TO CONTROL INSECTS PESTS IN CABBAGES PLANTATION (Brassica oleracea var. capitata L)." KnE Life Sciences 2, no. 1 (September 20, 2015): 405. http://dx.doi.org/10.18502/kls.v2i1.183.

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<p>Subculture Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is an entomopathogenic viruses isolated from cadaver of Helicoverpa armigera larvae, and was succesfully produced in Spodoptera litura larvae as an alternate host. These viral agent have been applied to control the population of three species insects pests Spodoptera litura, Crocidolomia pavonana and Plutella xyllostella which were infested in Cabbages Plantation. The concentration of virus of 4 x 107 Polyhedral/ml was sprayed in cabbage leaves 24 hours before infested of larval and every four days after. The research used randomized design which consisted of factor (three species of second instar larvae, S. litura, C. pavonana, P. xyllostella) and eight replications. The result showed that the three species of larval were sensitive against subculture HaNPV infection. The mortality of each larval species (S. litura, C. pavonana and P. xyllostella) were 100%, 97.5% and 98.7%. Subculture HaNPV can be utilized to control the population of three spesies of insects pests in Cabbage Plantation. </p><p>Keywords : Subculture HaNPV, Polyhedral</p>
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Gatehouse, L. N., N. P. Markwick, J. Poulton, and J. T. Christeller. "Avidin expression does not increase speed of kill of Spodoptera litura by baculovirus." New Zealand Plant Protection 56 (August 1, 2003): 194–200. http://dx.doi.org/10.30843/nzpp.2003.56.6042.

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A disarmed (polhminus) Autographa californica nuclear polyhedrosis virusbased baculovirus expressing green fluorescent protein (GFP) on the strong late promotor p10 was constructed A second virus was made which also expressed the biotinbinding protein avidin from the strong late promotor polh Both viruses caused strong fluorescence in Spodoptera frugiperda cells in insect cell culture Sevendayold larvae of Spodoptera litura were inoculated by microinjection with GFP virus GFPavidin virus or tissue culture medium (control) Mortality growth rate and the development of green fluorescence were followed and after death the larvae were analysed for GFP and avidin content All virusinjected larvae developed strong fluorescence However no significant differences in growth or mortality were seen between the larvae injected with virus expressing and not expressing avidin Both GFP and avidin accumulated to high levels in the larvae The expression of avidin did not change the level of expression of GFP
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Dissertations / Theses on the topic "Spodoptera Litura Nuclear Polyhedrosis Virus"

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Cartagena, Juan Carlos. "Contribution a l'etude de la sensibilite des insectes aux maladies infectieuses : influence de la fumure sur la sensibilite d'un insecte phytophage, spodoptera littoralis, boisduval (lepidoptera, noctuidae), a la polyedrose nucleaire." Paris 6, 1987. http://www.theses.fr/1987PA066152.

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On etudie la biologie du developpement et la sensibilite des chenilles de s. Littoralis a une polyedrose nucleaire. L'etude est realisee en fonction des fumures appliquees sans discontinuite chaque annee sur une culture de betteravess. Une carence azotee du regime alimentaire retarde le developpement des larves. Une deficience en phosphore provoque une diminution de la capacite reproductive des adultes. La sensibilite des chenilles a la virose est liee a la teneur de l'azote dans les feuilles et croit avec l'age des feuilles sur lesquelles elles s'alimentent
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Jones, K. A. "Studies on the persistance of Spodoptera littoalis nuclear polyhedrosis virus on cotton in Egypt." Thesis, University of Reading, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384156.

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Reeson, Andrew F. "Density dependent phase polyphenism in the African armyworm Spodoptera exempta (Lepidoptera: Noctuidae)." Thesis, University of Southampton, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297378.

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Dauthuille, Dominique. "Etude écopathologique de deux baculovirus pathogènes de Spodoptera Frugiperda (J. E. Smith) (lépidoptère : noctuidae) en prairie guyanaise à Digitaria Swazilandensis Stent." Paris 6, 1986. http://www.theses.fr/1986PA066484.

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Deux baculovirus, une granulose et une polyédrose nucléaire, isolés de S. F. En Guyane française sont décrits. Les deux maladies sont responsables d'enzooties dans les populations du ravageur en prairie à d. S. La valeur maximale de la mortalité larvaire naturelle engendrée par les deux virus, déterminée après échantillonnage et élevage au laboratoire, est de 32%. La cyclicité de la présence sur une même parcelle de la noctuelle et de ses baculovirus est due au mode de conduite des exploitations agricoles. Les taux de mortalité larvaire par granulose et par polyédrose nucléaire sont variables d'une parcelle à une autre, et un équilibre dynamique se maintient entre les deux viroses. L'introduction d'un mélange des deux virus a permis de montrer que les larves jouent un rôle important dans la dissémination des pathogènes dans la prairie.
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Lee, Shu-Tseng, and 李淑增. "Enhancing Factors of Nuclear Polyhedrosis Virus of Spodoptera litura and the Quantitative of Plutella xylostells Granulosis Virus." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/63596149658065483402.

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碩士
國立臺灣大學
植物病蟲害學系
84
The enhancing factors of SpltNPV was studied. In order to enhance the virulence of SpltNPV, we mixed several chemicals to the virus suspension to study the efficacy of enhancement. Our result revealed that SpltNPV was the most effective one against the Sl larvae. When we added the chemicals into the medium, the SL7B cell line could grow when the concentration was 0.01%. Therefore, we measured the TCID50 of SlECV with or without chemicals. The results showed several chemicals could lower the TCID50 of SLECV. The TCID50 of inoculant of SlECV was 3.09×10-8, bur, if we add 0.01 % sorbic acid or boric acid into the medium,the TCID50 could reduce to 1×10-8. However, 1% chemicals added to artifical diet were harmless to Sl larvae, the virus suspension containing 1% chemicals could increase the mortality of larvae. The mortality of larvae increased with increasing of chemicals' concentration in the virus suspension, except the fluorescent brightener 28. In addition to the chemicals, the granulosis virus（GV）could also enhance the effect of SpltNPV. The Kao Hsiung Plutella xylostella GV (PxGV) was the most effective to enhance the virulence of NPV. For estimating the concentration of GV, we established a method for purification and quantitation. The concentration of PxGV was estimated by mixing with latex and was observed by TEM. Better results were obtained when the latex diluted 100X. Absorbance of the PxGV mixture was measured by a spectrophotometer. The absorbance peak located between 200-300nm. The OD of different concentrations of PxGV were estimated under various wavelengths. The absorbance of PxGV increase with the concentration increasing and there was a significant relationship between absorbance and PxGV concentration. When comparing the absorbance at different wavelengths, the absorbance decrease with an increase of wavelength to estimate the concentration of PxGV.
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Lin, Ren Wei, and 林仁偉. "Construction of recombinant baculovirus containing melanin gene and the preliminary molecular studies on spodoptera litura nuclear polyhedrosis virus." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/07641768277165611775.

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鄭如茜. "Molecular cloning of polyhedrin gene from spodoptera exigua nuclear polyhedrosis virus." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/01846123010276182438.

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Chiu, Shu-Chun, and 邱淑君. "Gene expression of recombinant AcMNPV containing luciferase gene and the study of transfection of Spodoptera litura nuclear poly- hedrosis virus." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/87203117277970956167.

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碩士
國立臺灣大學
植物病蟲害學系
84
The luciferase gene from North American firefly was cloned to the AcMNPV transfer vector 1392, and cotrasfected with wild type AcMNPV DNA to SL7B cell line derived from Spodoptera litura ova- ries. The recombinant viruses were used to test the activities of of SV40 promoter and enhancer to regulate baculovirus gene expre- ssion. According to the difference in DNA fragment inserted, recombinant plasmid 1392 can be divided into four types, After cotransfection with 4 types recombinant plasmid DNA and wild type AcMNPV DNA te generate recombinant virus, plaque purificat- ion was used to purify recombinants. PCR and dot blot hybridization were used to indentify the inserts of each recombinant viruses. The four different recombinant viruses were obtained from different transfer vectors, and namely Acluc, Aclsp , Aclse, and Aclpe respectively. The time course of protein expr- essed in different 4 type recombinant viruses was ascertained by SDS- PAGE analysis. The results revealed that protein expressed was increase with the increased of time post infection. THe amo- unt and biological activity of protein expressed was checked by counting the light units of luminometer. The results revealed that recombinant Acluc containing only luciferase gene, obtains the highest protein expressed. The lowest protein expression was found in the recombinant Aclsp. In addition to recombinant virus study, we also tested the SlNPV DNA transfer to SL7B cells. The methods in our study included calcium phosphate coprecipitation, lipofection, and electroporation. Only lipofection obtained a positive result. After transfeciton, the PIBs were found in the nucleus of infected cell. The extracellular virus (ECV) DNA was extracted, and digested with EcoRI restriction enzyme to compare the RFLP between AcMNPV and SlNPV, and the results show that there were significant difference in these two kinds of viruses.
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Books on the topic "Spodoptera Litura Nuclear Polyhedrosis Virus"

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Seleksi berbagai jenis isolat nuclear polyhedrosis virus (NPV) yang paling virulen terhadap ulat bawang (spodoptera exigua hubn.): Laporan penelitian. Bandung: Lembaga Penelitian, Universitas Padjadjaran, 2000.

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Book chapters on the topic "Spodoptera Litura Nuclear Polyhedrosis Virus"

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Rajeshwari, R. "Mass Production of the Nuclear Polyhedrosis Virus (NPV) of Helicoverpa armigera and Spodoptera litura." In Biopesticides in Horticultural Crops, 185–94. London: CRC Press, 2021. http://dx.doi.org/10.1201/9781003243427-17.

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