Academic literature on the topic 'Splinkerette'

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Journal articles on the topic "Splinkerette"

1

Hengen, P. "Vectorette, splinkerette and boomerang DNA amplification." Trends in Biochemical Sciences 20, no. 9 (September 1995): 372–73. http://dx.doi.org/10.1016/s0968-0004(00)89079-9.

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Potter, Christopher J., and Liqun Luo. "Splinkerette PCR for Mapping Transposable Elements in Drosophila." PLoS ONE 5, no. 4 (April 13, 2010): e10168. http://dx.doi.org/10.1371/journal.pone.0010168.

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Horn, Carsten, Jens Hansen, Frank Schnütgen, Claudia Seisenberger, Thomas Floss, Markus Irgang, Silke De-Zolt, Wolfgang Wurst, Harald von Melchner, and Patricia Ruiz Noppinger. "Splinkerette PCR for more efficient characterization of gene trap events." Nature Genetics 39, no. 8 (August 2007): 933–34. http://dx.doi.org/10.1038/ng0807-933.

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Horn, Carsten, Jens Hansen, Frank Schnütgen, Claudia Seisenberger, Thomas Floss, Markus Irgang, Silke De-Zolt, Wolfgang Wurst, Harald von Melchner, and Patricia Ruiz Noppinger. "Erratum: Splinkerette PCR for more efficient characterization of gene trap events." Nature Genetics 39, no. 12 (December 2007): 1528. http://dx.doi.org/10.1038/ng1207-1528.

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Cavagnaro, Pablo F., Douglas Senalik, and Philipp W. Simon. "SplinkBES: a splinkerette-based method for generating long end sequences from large insert DNA libraries." BioTechniques 47, no. 2 (August 2009): 681–90. http://dx.doi.org/10.2144/000113122.

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Uren, Anthony G., Harald Mikkers, Jaap Kool, Louise van der Weyden, Anders H. Lund, Catherine H. Wilson, Richard Rance, et al. "A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites." Nature Protocols 4, no. 5 (April 30, 2009): 789–98. http://dx.doi.org/10.1038/nprot.2009.64.

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Too, W. C. See, Y. C. Liew, and L. L. Few. "Cloning of glyceraldehyde-3-phosphate dehydrogenase from an Antarctic psychrophilic bacterium by inverse and splinkerette PCR." Journal of Basic Microbiology 48, no. 5 (October 2008): 430–35. http://dx.doi.org/10.1002/jobm.200800008.

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Lynn, Elizabeth C., and Robert B. Beckstead. "Identification of Gene Expression Elements in Histomonas meleagridis Using Splinkerette PCR, a Variation of Ligated Adaptor PCR." Journal of Parasitology 98, no. 1 (February 2012): 135–41. http://dx.doi.org/10.1645/ge-2916.1.

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See Too, W. C., and L. L. Few. "Cloning of triose phosphate isomerase gene from an antarctic psychrophilic Pseudomonas sp. by degenerate and splinkerette PCR." World Journal of Microbiology and Biotechnology 26, no. 7 (January 3, 2010): 1251–59. http://dx.doi.org/10.1007/s11274-009-0295-9.

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Cleveland, Susan M., and Utpal P. Dave. "Insertional Activation of GLI2 in Adult T-Cell Leukemia/Lymphoma." Blood 110, no. 11 (November 16, 2007): 4149. http://dx.doi.org/10.1182/blood.v110.11.4149.4149.

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Abstract Retroviruses induce cancer by integrating into the cellular genome and activating oncogenes or inactivating tumor suppressor genes. Human T-cell Leukemia Virus type 1 (HTLV-1), a complex retrovirus, induces Adult T-cell Leukemia/Lymphoma (ATLL) after a latency of over 30 years and in only 5% of carriers. The long latency and incomplete penetrance is similar to how slow transforming retroviruses induce cancer in mice and imply multiple oncogenic “hits” need to accumulate for clinically apparent disease. Insertional mutagenesis may be one mechanism by which ATLL develops. We used splinkerette-PCR to clone and map insertion sites from an HTLV-1 infected T-cell line, Hut-102. We identified an HTLV-1 insertion 5′ of the GLI2 gene, formerly known as Tax-Helper-Protein-1. We found GLI2 was up-regulated by promoter insertion. Interestingly, we found GLI2 protein occupied the HTLV-1 Long Terminal Repeat. The effect of GLI2 expression on viral expression was investigated by knockdown of GLI2 in Hut-102 cells. Our results show that retroviral insertional mutagenesis can be an important mechanism in HTLV-1-induced leukemias and lymphomas.
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Book chapters on the topic "Splinkerette"

1

Yin, Bin. "Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures." In Methods in Molecular Biology, 25–42. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-944-4_3.

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2

"Splinkerette." In Encyclopedia of Genetics, Genomics, Proteomics and Informatics, 1862. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_16009.

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