Journal articles on the topic 'Spirit of aloha'

The meaning of Kekuni expresses the mana of a special healing light from the fire of the lama wood, which carries the power to reverse negativity. For those of us who knew Kekuni Blaisdell and remain close in spirit, and for those who are forever affected by his light, we have all felt the impact of his aloha, his spirit, his soul, and that eternal light in our lives. In honor of Kekuni and all that he stood for, worked for, and manifested, we honor him most by carrying that light forward in our collective lives, work, and continuing kuleana of aloha ‘āina, ‘āina aloha...

King’s Hawaiian has been family owned for more than six decades, and the ‘aloha spirit’ runs deep. But, rapid growth in recent years has exposed serious challenges. From production inefficiencies to supply chain disruptions, management recognizes the need to rapidly evolve their family business to a professionally managed one. Their relationship-based supply chain worked for a family firm, but will this approach continue to work as they expand rapidly? A disruption in their egg supply exposed the firm to risks they never anticipated. Now, they are rethinking their entire supply chain, including their most important raw material: flour. How can they strategically manage their raw material needs while sustaining double-digit growth? How do they identify and mitigate risks in their chain? King’s Hawaiian knows its success is in its supply chain, including their unique delivery model. Getting this right is critical to their mission: to deliver their aloha spirit to every household, every day.

Many people think of Hawaii only in terms of the enthusiastic “Aloha Spirit”, but have very little knowledge of the development and evolution of Hawaiian art. This research paper analyzes the influence of the Hawaiian environment and immigrants from different cultural backgrounds on the development of Native Hawaiian art in three areas: music, painting, and sculpture.

AbstractThis article studies the Hawai‘i tourism industry's efforts to market Hawai‘i as a multi-cultural paradise where positive racial experiences could be bought and sold. With jet travel arriving in Hawai‘i the same year as statehood, the tourism industry, aided by the new state government, exploited Hawai‘i's newfound prominence, luring planeloads of American tourists who thronged its beaches, hotels, and cultural spectacles. Tourism helped turn racial tolerance into a saleable, if intangible, commodity. Marketers invited mainlanders to partake in the islands’ celebrated ‘Aloha Spirit’: an elusive vision of social harmony said to be the defining feature of the Hawai‘i vacation. By attending ethnic festivals, eating exotic food, and interacting with locals, visitors might even bring some Aloha Spirit home with them. Hawai‘i's society thus became not only a site of consumption, but an object of consumption itself. What such utopian ideas obscured was that the broader construction of Hawai‘i as a multi-cultural paradise was part of state efforts both to transform Hawai‘i's economy and to promote US influence in the Pacific. While the limited historiography on multi-culturalism situates its emergence in grassroots protest, this article argues for the elite origins of the multi-cultural ideal, which served the interests of both business and US foreign policy.

El surf es una práctica que cada vez acapara más relevancia en nuestra sociedad. Esta práctica tiene en multitud de comunidades implicaciones socioeconómicas que son cada vez más importantes ya que el aumento de practicantes ha conllevado también un aumento de empresas que tratan de prestarles servicios. Muestra de su creciente relevancia social es que cada vez son más los medios de comunicación generalista que dan cobertura a noticias vinculadas con el deslizamiento sobre las olas, en especial, seguramente por su estética radical, a los hitos alcanzados por los surfistas que cabalgan olas gigantes. El crecimiento de practicantes de surf se vincula con la representación fenomenológica que tiene de muchos de los valores aspiracionales de la sociedad contemporánea como la libertad, la vida en la naturaleza o el hedonismo. Valores asociados al espíritu Aloha, al cual se vinculan los orígenes del surf. Sin embargo, los hallazgos realizados tras una revisión bibliográfica de varios de los principales textos bibliográficos sobre la historia y cultura surf, nos llevan a la conclusión de que parece evidente la existencia de un interés en ofrecer una visión romántica de los orígenes del surf. El nacimiento del surf, aunque muchos historiadores pretendan negarlo, es una total incógnita, sin embargo, vincularlo con raíces polinesias ha servido para moldear la imagen paradisíaca socialmente construida que el marketing ha logrado perpetuar. Abstract. Surfing is a practice that is becoming increasingly important in our society. This practice has socioeconomic implications in many communities that are increasingly important since the increase in practitioners has also led to an increase in companies that try to provide them with services. An example of its growing social relevance is that more and more general media are covering news related to surfing the waves, especially, surely due to its radical aesthetics, the milestones reached by surfers riding giant waves. The growth of surfers is linked to the phenomenological representation that they have of many of the aspirational values of contemporary society such as freedom, life in nature or hedonism. Values associated with the Aloha spirit, to which the origins of surfing are linked. However, the findings made after a bibliographical review of several of the main bibliographical texts on the history and culture of surfing, lead us to the conclusion that the existence of an interest in offering a romantic vision of the origins of surfing seems evident. The birth of surfing, although many historians try to deny it, is a total unknown, however, linking it with Polynesian roots has served to shape the socially constructed paradisiacal image that marketing has managed to perpetuate.

This article addresses three issues of what I suggest here should be regarded as the shifting technological and matter-based apparatus of animation: first, the meanings and affect of objects and materials actually used in animated films; second, the visual dramaturgy made possible by objects and materials for animation screenwriters; and, third, the status of animation process materials as archival objects. The analysis looks at a number of animated films and specifically at their design form, material association, and narrative function to define what I will call the “scripted artefact”, and an “Animated Object Cycle”. This overview will also operate in a spirit of thinking about theories of practice and practices of theory in animation, and refer to both established theoretical perspectives as well as primary practice idioms.

When I conceptualised the Sisters of the Screen project as a book and film, I envisioned an “imagined community” of kindred spirits, a “sisterhood” where the screen was their ultimate point of convergence. The screen is where their images are read; whether it’s a movie screen, television set, video monitor, computer screen, tablet or mobile phone, for a director, producer, film festival organiser, actor, critic or spectator the screen is the ultimate site where the moving image is viewed, interpreted and understood. With the phenomenal development of screen culture as a result of the digital turn, I return to the “screen” as a conceptual framework that integrates screen media, and their associated devices and technologies; hence, the concept “African women of the screen” as the organising principle. This report examines the impact of the digital turn on African women of the screen, how their cinematic gaze has evolved, developed and transformed with the evolution of new technologies such as the Internet and, in particular, the emergence of social media.

Dark Spirit Adventure is a 2D puzzle platform game developed for Windows PC. This game was created using Unity game engine with C# as a programming language. The goal of this game is to complete all the stages and collect all the collectibles. At each stage, players will face various obstacles such as enemies, puzzles, and lights. Testing is done by blackbox testing, alpha testing by the supervisors, and beta testing by 31 respondents through an online questionnaire. The test results show that the game Dark Spirit Adventure entertains players, challenging players and has high replay value.

The goal of this paper was to identify the leadership approach and dimensions influencing the managerial perceptions of the agricultural organization’s president. This research was carried out with agricultural organization presidents by using questionnaires. For this purpose, 29 items were given to presidents and a Likert scale used for the degree of agreement of each item. Factor analysis, Cronbach alpha coefficient was situated to be 0.933. To specify the dimensions influencing the managerial perception of agricultural organization’s president principle component analysis with varimax rotation was used. Eigenvalue belongs to seven of 29 items was calculated higher than one. As a result of this analysis, seven dimensions were examined. These dimensions clarified 75.75% of the total variance. The main aspects influencing the managerial perception of agricultural organization’s president were constructed to be motivation and team spirit, the power of initiative, put the organization interests over their individual interests, devotion to duty, entrepreneurial spirit, empathy ability and sense of belonging to an organization.

This study examined if poor officiating, lack of sportsmanship spirit and poor facilities are triggers of violence among sports audience in Port Harcourt Metropolis, Nigeria. This study was conducted among the inhabitants of Port Harcourt metropolis in River state. The descriptive survey research design and a sample size of 1,000 participants were used. The multi-stage sampling technique, a structured, validated and modified Likert type questionnaire were employed. The reliability of the instrument was determined through the Crombach Alpha, which gave a value of r = 0.89. The data collected was analyzed using the Chi-square statistics. The study revealed that poor officiating, lack of Spirit of Sportsmanship and poor facility were triggers of violence among sports audience in Port Harcourt metropolis, Nigeria. It was consequently recommended that only competent and knowledgeable officials should be used for matches. Both players and supporters should be educated on the rules of games and trained to accept the decision of referees’ umpires etc. and should not see competition as a do-or-die affair/event.

The purpose of this research was to study deterring factors to entrepreneurship among graduates of agriculture and natural resources. A survey approach was used in this research. The statistical population included ninety eight graduates of agriculture and natural resources who are working in private or governmental business units in the province of Bushehr, Iran. A questionnaire was developed to interview the subjects of the study of which the validity and reliability were estimated based on the opinions of a panel of experts and Cronbach’s alpha coefficient respectively. The results indicated that among personality factors, low self-confidence and low spirit of independence were the most important deterring factors to entrepreneurship. The results also revealed that a weak relation between university and business environment, insufficient practical and theoretical education, high risk of investment in the agriculture, and legal and administrative restrictions were the most important environmentally deterring factors. Based on this research finding, male graduates had a higher spirit of opportunity recognition and independence than female graduates.

We present a theory of large-scale dynamo action in a turbulent flow that has stochastic, zero-mean fluctuations of the ${\it\alpha}$ parameter. Particularly interesting is the possibility of the growth of the mean magnetic field due to Moffatt drift, which is expected to be finite in a statistically anisotropic turbulence. We extend the Kraichnan–Moffatt model to explore effects of finite memory of ${\it\alpha}$ fluctuations, in a spirit similar to that of Sridhar & Singh (Mon. Not. R. Astron. Soc., vol. 445, 2014, pp. 3770–3787). Using the first-order smoothing approximation, we derive a linear integro-differential equation governing the dynamics of the large-scale magnetic field, which is non-perturbative in the ${\it\alpha}$-correlation time ${\it\tau}_{{\it\alpha}}$. We recover earlier results in the exactly solvable white-noise limit where the Moffatt drift does not contribute to the dynamo growth/decay. To study finite-memory effects, we reduce the integro-differential equation to a partial differential equation by assuming that ${\it\tau}_{{\it\alpha}}$ be small but non-zero and the large-scale magnetic field is slowly varying. We derive the dispersion relation and provide an explicit expression for the growth rate as a function of four independent parameters. When ${\it\tau}_{{\it\alpha}}\neq 0$, we find that: (i) in the absence of the Moffatt drift, but with finite Kraichnan diffusivity, only strong ${\it\alpha}$ fluctuations can enable a mean-field dynamo (this is qualitatively similar to the white-noise case); (ii) in the general case when also the Moffatt drift is non-zero, both weak and strong ${\it\alpha}$ fluctuations can lead to a large-scale dynamo; and (iii) there always exists a wavenumber ($k$) cutoff at some large $k$ beyond which the growth rate turns negative, irrespective of weak or strong ${\it\alpha}$ fluctuations. Thus we show that a finite Moffatt drift can always facilitate large-scale dynamo action if sufficiently strong, even in the case of weak ${\it\alpha}$ fluctuations, and the maximum growth occurs at intermediate wavenumbers.

Abstract Caregivers of older adults are at risk for poor physical and mental health outcomes. Chinese American caregivers, especially those who are recent immigrants, are at risks for developing health symptoms while less likely to seek outside help and utilize caregiver resources due to cultural barriers, low health literacy, and limited-English proficiency. This symposium will introduce the Caregiver Self-Management Program (CSMP), a training program based on the Body-Mind-Spirit model to equip Chinese immigrant caregivers with self-care knowledge, skills, and self-efficacy. We designed the in-person training to be four 3-hour sessions and pilot tested it among 11 Chinese caregivers. The training was well-received by participants; however, the caregivers’ duties and their working status, compounded with transportation barriers, leave them little time to participate in the intervention. To address these challenges, we further employed a user participatory approach to design, prototype, and pilot-test a CSMP mobile application (app) to meet the needs of Chinese immigrant caregivers. App functions included interactive multi-media lessons and practice, scheduling of coaching sessions for problem solving, extended readings, community resources, etc. We co-designed the app with seven caregiver co-designers, alpha-tested the app prototype with 12 caregivers, and beta-tested the app in real-life settings with 20 caregivers. Four presentations in the symposium will discuss the CSMP in-person training design and evaluation, influence of Chinese culture on caregiving and impacts of CSMP, user-participatory approach used in co-design and alpha-testing of the app prototype, and preliminary results from the beta-testing to assess external user acceptance and feasibility of the CSMP app.

The purpose of this research is to know the level of financial literacy learning model effectivity for building quality character of sekkah student base The effectivity levels of the models will be used to determine whether the model can be used as best practical or not. Number of population of 272 and it all made as the respondents, thus this research is the research population. Data analysis using t-test and tools to validate using the kendall and spearman, while for reliability using cronbach alpha as its parameters. The results showed the existence of level models effectivities at the level of significance of 1%, the components of the planning, the implementation and evaluation has an impact on the success of the formation of nonkonsumerisme character and spirit of entrepreneurship.

In 1911, Schur published a rather formidable paper [9] in which he determined all the complex projective characters for the symmetric group (denoted Σn here, despite the title), and for the alternating group An (A pronounced “alpha”). As far as we know, the construction of the modules involved is still an unsolved problem. The results of Schur can be expressed in terms of certain induced representations whose characters form a basis for the group of virtual characters, plus formulae expressing the irreducible characters in terms of these induced characters. Here we give a new formulation of the above induced characters in the spirit of the well known “induction algebra” approach to the linear representations of Σn. We use some Hopf algebra techniques inspired by [5] to give new proofs of Schur's results, and to determine the extra structure which we define.

The current study aims to identify the contribution of Arabic middle-school curricula in the KSA in developing risk management skills from the view of teachers in light of Covid-19 pandemic. The sample consisted of 240 Arabic teachers in Khamis Mushait department of education. A questionnaire was used as the study tool. The questionnaire consisted of 5 dimensions and 24 indicators. Pearson correlation, Alpha-Cronbach, average, relative weights of averages, columns, standard deviation, and the outweighing relative weight of averages. The main results were: there is a medium contribution for the skills of facing stress and innovative thinking, and a low contribution for courses in the following skills: effective contact and communication, boosting team spirit and collective work, and time management. In light of results, the researchers provided some recommendations: reconsidering Arabic curricula to raise awareness of crises management skills and relevant concepts.

The current study was conducted to examine the internal structure of the 1983 Participation Motivation Questionnaire of Gill, Gross, and Huddleston, a measure of motives for participating in sport and physical activity, when used among undergraduates. Principal component analysis of responses from students yielded a six-factor solution. The subscales were team orientation, achievement/status, fitness, friendship, skill development, and fun/excitement/challenge. The internal consistency for the subscales was generally adequate (coefficients alpha ranged from .67 to .93). Descriptive statistics indicated that the students' most important motives for participating in physical activity were to (a) maintain fitness, (b) experience fun, excitement, and challenge, and (c) acquire and improve skills. The least important motives for participating in physical activity were to (a) maintain and develop friendship, (b) obtain achievement and status, and (c) experience teamwork, team spirit, and being on a team. Avenues of research are discussed.

Aromatherapy is an ancient healing process that uses pure aromatherapy plant extracts aimed at improving the health and well-being of the body, mind and spirit. Lavender contains linalil acetate and linalool. Linalil acetate and linalool have no harmful side effects on health. This substance is antibacterial, fungicidal, viriside, parasiticidal and vermifugal and has neurotonic and uterotonic action. This study aims to determine the effect of lavender oil (Lavandula angustifolia) aromatherapy candles on increasing the concentration of student learning at the Madrasah Tsanawiyah Al-Wasliyah Pasar Miring in Desa Pasar Miring . The study used a quasi-pretest-posttest experimental method for 30 randomly selected students. The instrument in this study used the Army Alpha Test with data analysis using Paired T-Test. Hypothesis testing using Paired T-Test showed p value = 0.026<0.05 (p <0.05). It can be concluded that there is an influence of lavender oil aromatherapy candles on increasing the concentration of student learning in Madrasah Tsanawiyah Al-Wasliyah Pasar Miring in Desa Pasar Miring

Abstract Abstract 2192 Poster Board II-169 Thanks to its striking effectiveness, imatinib (IM) is the current standard of therapy in chronic myeloid leukemia (CML). However, Interferon-alpha (IFN-alpha) has induced a low but reproducible curative effect in some CML patients, inducing durable remissions lasting even after therapy discontinuation. For this reason, the interest on the possible use of IFN-alpha in the treatment of CML is still substantial, and very recently some newer prospective studies from the French and the German Groups have proposed the re-introduction of IFN-alpha in the front-line treatment of early chronic phase CML (ECP-CML), in association with IM (Rousselot et al, Haematologica 2009;94:abs.1093; Hehlmann et al, Haematologica 2009;94:abs. 0478). We compared the response of 495 ECP-CML patients, enrolled into three different prospective studies promoted by the GIMEMA CML WP (419 treated with imatinib 400mg and 76 treated with imatinib 400mg plus IFN-alpha) (study protocols NCT00511121, NCT00514488 and NCT00511303). Cytogenetic analysis was performed by standard banding techniques and cytogenetic response was rated according to the European LeukemiaNet guidelines. Molecular response was assessed on peripheral blood cells by a standardized quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR) method on an ABI PRISM 7700 Sequence Detector (Perkin Elmer, Faster City, CA). BCR-ABL transcript levels were expressed as a percentage according to the IS. Patients were equally distributed by Sokal risk in the two cohorts. Median follow-up was 43 mos (range, 12- 67) in the IM group and 54 mos (range, 11-63) in the IM+IFN-alpha group. Compliance to IM was excellent in both groups, with 90 to 95% of patients receiving IM 400 mg/daily throughout the follow-up. Conversely, the proportion of patients continuing IFN-alpha dropped from 41% at 12 mos to 18% at 18 mos, 13% at 24 mos, 3% at 36 mos; by the end of the fourth year, all patients were off IFN-alpha. The number of patients in CCgR was higher in the IM+IFN-alpha than in the IM group at 6 mos (60% vs 42%, p=0.002) but not from 12 mos on (Table 1). The number of patients in MMolR was higher in the IM+IFN-alpha than in the IM group at 6, 12 and 24 mos, but not later on (Table 2). Also the number of patients with undetectable Bcr-Abl transcript levels was higher in the IM+IFN-alpha group at 12 months (15% vs 5%, p=0.003) but not later on (19% vs 18% at 48 mos). These data support the preliminary analysis of the French SPIRIT group, reporting a higher rate of MMolR after initial treatment with IM and IFN-alpha as compared to IM alone. The loss of the difference from 24 mos on could be explained by the fact that almost all patients had discontinued IFN-alpha during the first two years, pointing out that the low compliance to the combination may limit its utility in practice. ACKNOWLEDGEMENT The Italian Association Against Leukemia-lymphoma and myeloma (BolognAIL), The Fondazione del Monte di Bologna e Ravenna, The Italian Ministery of Education (PRIN 2005, No. 20050 63732_003, and PRIN 2007, No 2007F7 AE7B_002), The University of Bologna, The European Union (European LeukemiaNet). Disclosures: Pane: Novartis: Research Funding; Ministero dell'Università/PRIN: Research Funding; Regione Campania: Research Funding; Ministero della Salute/Progetto integrato Oncologia: Research Funding. Saglio:Celgene: Honoraria; Novartis: Honoraria.

This paper deals with the connection between the origin of the human race and the evolving universe in the works of Teilhard de Chardin, the French thinker analysed this problem from different points of view: scientific, philosophical and theological, showing its different aspects.The results of his reflections on this topic form a system of thought in which Teilhard tried to explain the mystery of man and the universe, the main concept of this system is the evolution of the whole universe from a material into a spiritual reality. Part of this cosmic evolutionary process is also the origin of the human race (considered by Teilhard as a species of living creatures and only accidentally as individual human beings). Creation of the world and man according to Teilhard is also a continual process in which God uses the natural law of evolution. Man is the best part and the summit of this cosmic process, the human race has been craeted by God as one philum (monophiletism) and not as a couple (Adam and Eve in the garden of Eden) or many phila (poliphiletism). While creating human souls, God also uses the material elements of the world, sublimating them into spirit, these opinions created many problems for the Catholic Church and were not accepted. Teilhardian analyses of the future of the universe and mankind are very interesting and inspiring and have been used by many modern thinkers. Teilhard wrote about one global society united by science and technology (globalisation). In the future people will also form one sphere of human spirit, the sphere of common information (noosphere). Eschatologically, the whole universe along with the human race will be united with God as the mover and final cause of the cosmic evolution (its point Alpha and Omega).The end of the history of all created reality will be the transformation into spiritual reality of the Cosmic Christ, thus anthropogenesis will be fulfilled in cosmogenesis and finally in Christogenesis.

AbstractIn this paper, we use the rearrangement-free argument, in the spirit of the work by Li, Lu and Zhu [25], on the concentration-compactness principle on the Heisenberg group to establish a sharpened version of the singular Lions concentration-compactness principle for the Trudinger–Moser inequality in{\mathbb{R}^{n}}. Then we prove a compact embedding theorem, which states that{W^{1,n}(\mathbb{R}^{n})}is compactly embedded into{L^{p}(\mathbb{R}^{n},|x|^{-\beta}\,dx)}for{p\geq n}and{0<\beta<n}. As an application of the above results, we establish sufficient conditions for the existence of ground state solutions to the followingn-Laplace equation with critical nonlinearity:($*$){}\left\{\begin{aligned} &\displaystyle{-}\operatorname{div}(|\nabla u|^{n-2}% \nabla u)+V(x)|u|^{n-2}u=\frac{f(x,u)}{|x|^{\beta}},\\ &\displaystyle u\in W^{1,n}(\mathbb{R}^{n}),\quad u\geq 0,\end{aligned}\right.where{V(x)\geq c_{0}}for some positive constant{c_{0}}and{f(x,t)}behaves like{\exp(\alpha|t|^{\frac{n}{n-1}})}as{t\rightarrow+\infty}. This work improves substantially related results found in the literature.

Early Saturday morning on 16 May 1998, fifty people made their wayinto the conference room of Georgetown University’s SalaamIntercultural Center for the first annual conference of the Association ofContemporary Muslim Philosophers. Looking into their eyes, one couldsee a glimmer of hope and the fire of enthusiasm. Clearly, this was notgoing to be a run of the mill encounter of Muslim minds. Some of thegreatest intellects of the Muslim world were present, among themProfessor Seyyed Hossein Nasr, Dr. T a B JBbir a1 ‘AlwBni, and Dr. KamalHassan. Young students with fresh countenances and effervescent commentswaited to deliberate upon such issues as the difference betweenpublic and private philosophy, the role of postmodernism in the Muslimworld, and why and in what condition does the Muslim intellectual traditionfind itself.Ironically, this occurred in view of an immense Jesuit Crucifix heraldedby the Greek letters alpha and omega, which symbolize Christ.While for some this signified the contradiction and turmoil presentwithin current Muslim philosophical discourse, for others it embodieda promising message. For those who saw it as a positive symbol,including myself, the cross served to illustrate the universe, markingthe four cardinal directions of space, and the surrounding alpha andomega symbolized the all-encompassing nature of the Qur’an. On aterrestrial level, it verified the resilient nature of the Muslim intellectfor, quite obviously, we were a group of Mhims meeting in a Jesuitinstitution to talk about reviving Islamic philosophy. Nevertheless, theuniversal significance of that symbol was realized by the spirit of thegathering and in the profound discussions afforded by all those present.The conference started with a moment of reflection upon the versesof the Qur’an found in Siirat a1 ‘Alaq: ...

Juliusz Słowacki and Teilhard de Chardin did not have much in common. The former was a Polish poet who wrote in the first half of the 19th century using a literary-pictorial style. The latter, on the other hand, was a French philosopher working in the first half of the 20th century using a scientific and intellectual style. In spite of these differences, one may get the impression that they both followed the same goal: to learn and explain the principles of the development of the world, from its origin to its end, from Alpha to Omega. This aspiration was accompanied by a belief (in Słowacki’s case, a messianic one) that the progress of existence leads to salvation and takes place according to a certain plan. One of the main mecha- nisms of this plan is the process of lifting the consciousness through the evolution of various biological forms towards its final shape – unity with God who is both a person and the absolute which encompasses all of the creation. Although the poet and the philosopher used different communication codes, their works share a common vision of evolution as a transition from an unconscious, dispersed exist- ence to a united being in which the spirit, the knowledge and the mind can achieve a “global”, yet personalized level.

The purpose of this study was to develop a rating scale that can be used to evaluate behavior patterns of the organization people pattern of preservice teachers (PSTs). By reviewing the related literature on people patterns, a preliminary scale of 38 items with a five-points likert type was prepared. The number of items was reduced to 29 after obtaining expert opinions and was administered to 620 PSTs. As the results of the exploratory and confirmatory factor analysis, unlike two factors: structurist and free spirits behavior patterns, in the theory, we obtained the final scale of 15 items consisting of three factors: planners, solution-oriented and prescriptive behavior patterns. The related Cronbach Alpha value was found to be .830 for all the items. We identified that behavior patterns rating scale of the organization people pattern can be confidently applied to evaluate behavior patterns. Moreover, in this study, we obtained a contradiction between practice and theory. Thus, we provided a new idea related to behavior patterns of the organization people pattern.

Within a hegemonic Western discourse, Hawai‘i is largely considered an aesthetic tourist destination. It is perceived to be a vacation haven, bountiful in opportunities for real estate, commodification, and gentrification. While endeavors such as these have indeed proven to be economically prolific for the state, the profits do not directly, if even remotely, benefit the Native Hawaiians whose land continues to be seized and commodified in the name of said profits. Therefore, that dominant discourse which paints Hawai‘i as a tourist destination of great economic potential is in fact a colonialist notion, denoting Hawaiian land as public property to be seized, altered, and owned. In reality, the land that is used for expansive capitalist ventures is often seized from Native people, as has been the trend since settlers first invaded Hawai‘i. This truth is further troubling when one considers Native Hawaiian land relations and the spiritual connection that many Kānaka Maoli (Native Hawaiians) have to that land. In light of the spiritual ties that many Kānaka Maoli have to land, or 'āina, its seizure and alteration by non-Native persons is an act of colonialism against not only Kānaka Maoli homes, but also against our bodies and spirits. This spiritual and emotional connection between Kānaka Maoli and our land is deeply rooted, and it is an idea commonly expressed in contemporary Kanaka culture by the term aloha ʻāina. In understanding this sentiment, it is essential that one first understands that aloha carries a much deeper meaning than the Hawaiian “hello” and “goodbye.” Aloha connotes one’s deep love for and connection to Kanaka culture. It also signifies love for one’s neighbors, friends, and ancestors. In essence, aloha ʻāina is an expression of one’s identification with and commitment to Hawaiian land and its connected historical and cultural significance. By close reading Kanaka texts and terminology such as this, one can begin to understand the sanctity of Kanaka land relations, thereby lending to an understanding of one of the ways by which colonialism against Kānaka Maoli continues in perpetuity. In this thesis, I will investigate and discuss relationships between Kanaka bodies and ‘āina. I will do this by close reading Kanaka literature, including the Hawaiian creation mele known as the Kumulipo, the narrative and performative device that is hula, and my Auntie Betty's stories that have been passed down to me through oral storytelling. I will contextualize my findings in both historical and contemporary frames. Ultimately, I am conducting this research with the aim of contributing to existing scholarship which aims to dismantle the dominant narrative which suggests that we live in a post-colonial era. The idea that colonialism is an extinguished historical event is a dangerous and false misconception that allows for the perpetuation of the discriminatory maltreatment of marginalized Indigenous communities and cultures. This discrimination is enacted in countless ways, including but in no way limited to the seizure of Kānaka Maoli lands. It is my hope that this research will encourage any and all readers to continue to learn about Kanaka Maoli and other Native cultures, and that this endeavor for further knowledge will lead to advocacy on behalf of, and greater reverence toward Native people, narratives, and knowledge.

Introduction: How patients cope with a new diagnosis of a serious/life threatening disease has been studied in a variety of settings and diseases, but not in acute myeloid leukemia (AML). In AML patients are confronted with a unique situation of a life-threatening diagnosis and the prospect of intensive prolonged treatments ahead of them. The aim of the current study is to assess different modes of coping with the diagnosis of AML, and whether these strategies remain stable over time. Methods: Adult patients with a new diagnosis of AML who were candidates for intensive chemotherapy and agreed to participate in this study were enrolled. The second author (Dr. Dorfman), a trained psychiatrist, and the chief of the Psycho-Oncology service in our institute, interviewed the patients at diagnosis and 3 months later. In these sessions the patients completed the Mental Adjustment to Cancer (MAC) questionnaire. The Hebrew version of this instrument was previously validated and consists of 40 items that assess the ability to cope with cancer and provides information on four major coping styles: fighting spirit, hopelessness/helplessness, anxious preoccupation and fatalistic acceptance. For each scale we calculated the standard (Z) score. The internal reliability measure (Cronbach's alpha) was above 0.7 for all scales. The sociodemographic interview gathered information on age, education, marital status, living arrangements, country of birth, religion and financial status. Additional medical information was obtained from the electronic medical record of each patient. We compared continuous variables using paired T-test and χ2 to compare categorical variables. We used a multivariate linear regression model to predict which of the baseline characteristics is associated with the coping strategy score. Results: We enrolled 18 patients with a recent diagnosis of AML. The median age was 55 years, and 12 (67%) were males. At time of diagnosis women, single patients (non-married or divorced), and patients with the lowest or highest income levels were significantly more anxious. Those who experienced a major life event within the previous year expressed lowers levels of hopelessness and a lesser degree of a fatalistic mode of coping. On the other hand, male patients, as well as unemployed or patients with low income, tended to express a higher degree of fatalistic feelings. Female patients were less likely to have high levels of fighting spirits. The mode of coping did not remain constant over time, and scores in all 4 scales changed over time. In fact, MAC scores in our cohort at 3 months correlated with baseline scores only in the fatalistic scale ( rp = 0.67, p = 0.006). Conclusion: In patients with newly diagnosed AML facing intensive chemotherapy, socioeconomics factors and life experience dictate, at least in-part, the coping strategy. However, 3 months after starting treatment, these factors become less influential. It is possible that at this stage, other factors, such as response to treatment, residual physical and mental resources and long-term prognosis become more important. Disclosures Wolach: Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees, Other: Speaker; AbbVie: Honoraria, Membership on an entity's Board of Directors or advisory committees, Other: Speaker.

Resilient economic development is supported by each and every people as a whole. To deal with disasters, we should not act alone. Readiness is one part of the process of disaster management and natural disasters that disrupt the living system. The study was conducted to measure the level of readiness for emergencies and disasters among youth (eg: high learning students) and to develop items for factors that influence students' ability to serve in disaster areas. To achieve the following objectives, two analyses were conducted: the first analysis was the comparison of mean scores and the second analysis was the factor analysis involving four factors, physical factors, university management factors, financial factors and personal factors. This study uses questionnaire and online form to collect data from respondents. A total of 120 respondents from three higher learning in Malaysia – Universiti Pendidikan Sultan Idris (UPSI), Universiti Sains Malaysia (USM) and University of Technology (UiTM) were involved in this study. The level of readiness has a high mean value and indicates a good level of readiness among the respondents. Factor analysis showed that there were no significant differences between the techniques of determining the number of factors or not for all the items that were formed. Nevertheless, the Cronbach Alpha values indicate that the items are constructed and that the overall research tool has internal consistency values. This study is expected to increase the engagement and spirit of volunteerism as it can add value to the students and even produce a high quality national leadership.

Inclusive education is based on the idea that all learners have to become participants in the process of (self-) education. This at first sight simple truth causes difficulties because of the variety of inborn human powers and in cases of learners with special needs, in particular. A wide range of strategies have been tested and applied for education of such children in Lithuania and all over the world. Lately significant attention has been laid on inclusive education. However, to achieve the desired outcomes, it is necessary to perceive methodological fundamentals and meaning of inclusive education to a teacher and a learner. Education is a specific activity, which is directed towards empowerment of an individual to spread own powers. Therefore, it is important to cognise the human nature and to derive the most significant approaches towards education from it. Contemporary science distinguishes three dimensions in the human nature, i.e., physical, psychical and spiritual ones, and their development is considered to be the main goal of a general education school. To enable appropriate attainment of this goal, it is necessary to perceive the essence of every dimension and their interrelation. The spiritual dimension causes the most serious concern. According to neurologist and psychotherapist V. Frankl, the genesis of the spiritual dimension is supernatural and enables an individual to transcendent the reality and to embody the existential freedom, whereof the real essence is revealed only through the interaction with truth (phenomenon of consciousness). An individual manages own physical and physical powers with the help of own spirit, is able to make decisions, to link own relations and activities with truth in an authentic way. The supreme expression of relations as well as that of spiritual dimension is love. The spiritual dimension is evoked and maturated by love of another individual, therefore, fruitful pedagogical support is always based on it. Moreover, V. Frankl points out that the spirit is never sick. Such approach to a human being enables us to understand why alpha and omega of education embrace spiritual powers of an individual, why teacher’s spiritual maturation becomes an important factor implementing the paradigm of free education, which contains inclusive education. It is of particular importance to learners with special needs because such education enables them to become full-value participants in the process of self-education in the most appropriate way to them, to experience success and to see the meaning of such activities. Healthy learners also feel advantages of inclusive education because it enables them to self-assess own potential and to envisage their real meaning in a new light. Inclusive education is relevant to teachers as a certain challenge to their spiritual as well as professional capacities, which reveals the in-depth essence of their activities.

Applications of teachers’ pedagogical approaches for learners’ educational gains have been remained one of the educational debates since ago. Teachers transfer chunk of information among students through implementing techniques to strengthen teaching learning process. Relevance of ground-breaking instructional strategies put concrete effect on students’ entire academic success. Present quantitative research was conducted to explore the effect of teachers’ usage of students’ centered teachers’ pedagogical approaches on students’ educational gains. Researchers adopted ex-post-fact research design on sample of 800 respondents; 400 urban and 400 rural students randomly selected from public sector secondary schools of District Kasur Punjab-Province of Pakistan. Data were collected by administering self-constructed questionnaire mode of 5-point Likert type options. Validity of the instrument was confirmed from experts and Cronbach’s Alpha was applied to ensure instrument’s reliability scores; .864. Learners’ educational gains of science and arts students were obtained from gazette of Board of Intermediate and Secondary Education, Lahore. Results of independent sample t-test and regression analysis ascertained that teachers’ centered students’ pedagogical approaches effect 58% on learners’ educational gains whereas brainstorming 43%, inquiry 57%, problem solving 54% and cooperative technique effect 50% on learners’ educational gains. Science teachers were making more use of brainstorming, inquiry, problem solving, and cooperative teaching as compared to arts teachers. Study recommends that Govt. may train heads of the institutions on students’ centered teachers’ pedagogical approaches and bound them to implement with true spirit and vigor through teachers to overcome science and specially arts students’ declining level of educational gains; stakeholders’ cause for concern. Keywords: brainstorming, cooperative teaching, inquiry base, learners’ educational gains, problem solving.

Abstract Of the few studies that have examined alcohol consumption in relation to risk of renal cell cancer (RCC), most are case-control studies. The extent to which alcohol affects RRC risk is unclear. We prospectively examined the association between total alcohol intake as well as specific types of alcoholic beverage and RCC in a large cohort of Finnish male smokers. Men from the Alpha-Tocopherol, BetaCarotene (ATBC) Cancer Prevention Study were followed for 12 years and RCC cases were identified. Alcohol consumption was assessed at baseline using a questionnaire previously shown to be both reproducible and valid. Cox proportional hazards modeling was used to adjust simultaneously for known or suspected risk factors for RCC. We ascertained 195 incident cases of RCC. In multivariate analysis, the relative risks and 95% confidence intervals (CI) of RCC according to increasing quartiles of total alcohol intake were 1.0, 0.91 (0.62-1.33), 0.94 (0.64-1.38), and 0.53 (0.34-0.83), respectively (P value for trend = 0.005); for spirit consumption, 1.0, 0.93 (0.63-1.Fspiait39), 0.84 (0.58-1.20), and 0.55 (0.36-0.85) (P for trend = 0.02); and for beer intake, 1.0, 1.22 (0.85-1.76), 0.83 (0.57-1.22), and 0.55 (0.36-0.85) (P for trend = 0.003). Too few people in this cohort drank wine to assess its association with risk of RCC. These data suggest that alcohol consumption is associated with decreased risk of RCC in male smokers. Because most of the risk reductions were seen at the highest quartile of alcohol intake and alcohol is a risk factor for a number of cancers particularly among smokers, these data should be interpreted with caution.

Introduction: Holistic care focuses on the relationship between the body, mind, and spirit. Spiritual needs are among the basic needs of the individual. From the physical and spiritual dimension and the interaction of these two dimensions, the spiritual needs of the person are developed. Aim:The aim of our study was to analyze if the patient's knowledge regarding their diagnosis and possible proximity of death have significant impact on satisfaction of psychological and spiritual needs. Material and Methods:In our study we used standardized questionnaire The Spiritual Needs Assessment for Patient – SNAP constructed by Sharma Rashmi, applied for 113 respondents and processed by SPSS statistical program. Results:Reliability of questionnaire is given by Cronbach's Alpha 0.945 for the total SNAP. A statistically significant difference at the level p < 0.01 has been confirmed in all questions among respondents with fatal diagnosis and respondents with not life-threatening diagnosis. In domain of psychosocial needs, significant difference was not confirmed regarding to gender, age, education, income, religion and place of residency. In domain of spiritual needs, significant difference was partially confirmed regarding religion in 4 questions from 13. Conclusion:Faced with chronic or fatal diseases, many patients rely on dealing with spiritual and religious issues. In fact, spirituality/religiosity can be considered an important source of support and management of severe chronic diseases. It is possible to identify the four basic dimensions of spiritual need: interconnection, peace, meaning/purpose, and transcendence. Patients often have the problem addressing their needs for related psychosocial and physical problems such as physical disability, fatigue, sleep disorders, side effects of treatment, etc. Importance of satisfaction of psychosocial and spiritual needs is increased in time of threat, fatal diagnosis.

In the current Covid-19 pandemic, there is a process of major changes the result of social distancing policies for all people so that they must be able to adapt. Students must also adapt to changes in learning methods and have high morale so that they can continue to follow learning well. So students are required to have soft skills regarding the use of online learning model applications, preparing quotas, and being in an area that is sufficiently accessible to the internet network so that later there will be no obstacles when attending online lectures. Students must also adapt and have a fighting spirit to stay enthusiastic about learning. This research is a type of quantitative research, cross-sectional design with a descriptive-analytic approach. The sample was taken by random sampling technique of 200 respondents. as many as 175 people (88%). Talented respondents in the arts were 90 respondents (45%), sports were 70 respondents 35%), and other fields were 40 respondents (20%). The results of univariate analysis describe respondents with adversity quotient in the category climbers as many as 33 respondents (16.5%), the category campers obtained 163 respondents (81.5%) and the category quitters obtained 4 respondents (2%). Meanwhile, the frequency of self-adaptation ability was 193 respondents (96.5%) with the adaptive category and as many as 7 respondents (3.5%) in the maladaptive category. The results of the bivariate analysis showed that the Pv value was 0.001 less than the alpha value = 0.05, which indicated that there was a significant relationship between adversity quotient and the self-adaptation ability of students during the Covid-19 pandemic with a positive relationship with sufficient strength. Further researchers are expected to research to discuss the factors and characteristics of self-adaptation that are dominant to adapt to a new environment. Keywords: Adversity Quotient, self-adaptation ability, covid-19

Fettgewebe ist kein passives Speicherorgan. Fettzellen sezernieren Hormone, Wachstumsfaktoren und Zytokine, sowie Komponenten des Komplement- und Gerinnungssytems. die nicht nur an der Regulation des Energiehaushaltes und anderer wichtiger Körperfunktionen beteiligt sind, sondern auch zur Entstehung von Gesundheitsstörungen beitragen können. So trägt, neben einer Dysregulation von PPAR-gamma möglicherweise eine Überproduktion von TNF-alpha im Fettgewebe Dicker zur Entstehung des metabolischen Syndroms bei. Fettgewebe liegt in Form definierter Depots vor, die sich biologisch unterscheiden. Bei Patienten mit Morbus Cushing oder AIDS kommt es zur Umverteilung des Fettgewebes, die im Falle von AIDS groteske Formen annehmen kann. Dies zeigt, dass sich die Zellen verschiedener Depots funktionell unterscheiden. Entgegen früheren Vorstellungen ist die Neubildung von Fettzellen aus fibroblastenähnlichen Vorstufen nicht auf kritische frühe Lebensphasen beschränkt, sondern ist bis ins hohe Alter möglich. Einmal gebildete Fettzellen müssen auch nicht lebenslang erhalten bleiben, sondern können durch Dedifferenzierung oder Apoptose wieder eliminiert werden. PPAR-gamma, ein ligandenregulierter Transkriptionsfaktor, der durch Fettsäuren und ihre Derivate aktiviert wird, spielt bei der Fettzelldifferenzierung eine zentrale Rolle und scheint auch an der Regulation der Fettzellgröße beteiligt zu sein.

Background: Task shifting has been recommended as a strategy to reach out to persons with mental illness and bridge the treatment gap. There is a need to explore task-shifting using existing health staff like Accredited Social Health Activists (ASHAs). Aim and Context: ASHAs are involved in ongoing community-based rehabilitation (CBR) program run with a public–private partnership over the last 5 years at Jagaluru Taluk (an administrative block) in Davanagere district (Karnataka, India). This article aims to summarize a randomized controlled trial (RCT) to examine whether CBR delivered by ASHAs is more effective than treatment as usual (TAU) control group in reducing disability associated with severe mental illness (SMI). Method: A group of proactive ASHAs is already working with us for a follow-up of persons with SMI. For the study, we would allocate areas that are currently not being covered proactively by ASHAs randomly in a 1:1 ratio via computer-generated randomization list to receive either ASHAs delivered CBR arm or TAU control group. A sample size of about 100 in each arm is enough to identify an effect size of 0.5 in total IDEAS score between the intervention and control arms with a power of 90% and an alpha of 0.05. We use the SPIRIT (Standard Protocol Items: Recommendations for Interventional Trials) statement to describe the methods of the trial. Result: The study has been approved by the institute ethics committee and registered with CTRI (CTRI/2019/08/020585 dated 6th August 2019). The recruitment of subjects is ongoing. The patients will be followed up for 1 year and assessed. The trial is funded by the Indian Council of Medical Research, Government of India. Discussion: The results of the study will be helpful from a public health perspective in delivering cost-effective and replicable CBR for persons with SMI through ASHAs. If the model turns successful, this could be expanded throughout the state/country. This would go a long way in bridging the huge treatment gap.

This article is a phenomenological interpretation of the myriad processes that influence the transient nature of ‘work’ and measures to retrieve the dignity it deserves. Vasu is an emerging organisational futurist whose passion is to develop management educational approaches that ensure a positive outlook of the uncertain future ahead. The differences between Eastern and Western history, especially Indian history is ingrained with a unique resilience to catastrophic events, invasions and embedded diversity. Leaning more in defence of human dignity at work according to Pablo Gilbert, Victor Frankl, World Happiness Report 2012 and the contemporary theory of autopoiesis, he conjectures a ‘FUTOPIA’ rising in the horizon, where humanistic valuing of work becomes culture. The corporate world is realizing the shifting paradigms—from end goals of profit-centric strategies and exploitation of human futures to partnerships with associates and ‘working resources’ that optimize creative contribution from work environments. At a meta level, the nation-building agenda of development is talking the language of equal opportunity for and treatment of women at work, with equitable wage parity and abolition of forced labour in the United States. Recent bills in the Indian Parliament on education and farmers’ rights and tailoring of the archaic labour laws will strengthen the negotiation for equitable fund allocation. The spirit of enterprise will boost small and medium sectors, especially farming and food product preservation, innovation and research and development (R&D), and rapid skill development will for sure retrieve the dignity tag for academics, farm labourers and those in the service sector, such as paramedics, the police, etc. According to thought leaders, corporate founders and contemporary authors, the future of work can be seen as a promising work in progress towards a new work ethic. This article risks suggesting radical steps needed in challenging traditional leadership styles and human resource (HR) practices of a growth economy that draws on patriarchal alpha-male prominence. What will replace traditional leadership styles is compassionate servant leadership, with leaders who will become designers of future ‘work’ environments.

Kajian bagi mengenal pasti penglibatan dan motivasi siswa guru di Institut Pendidikan Guru Malaysia (IPGM) dengan aktiviti-aktiviti berbentuk kesukarelawan terhadap golongan Orang Kelainan Upaya (OKU) amat diperlukan untuk penyediaan maklumat asas kepada pihak pentadbir IPGM dalam merangka program berbentuk kesukarelawanan untuk menarik minat siswa guru memberi sumbangan dalam bentuk khidmat masyarakat terutamanya kepada golongan OKU. Kajian deskriptif ini bertujuan untuk mengkaji motivasi penglibatan siswa guru dalam akiviti kesukarelawan membantu golongan OKU yang melibatkan 70 orang responden siswa guru dari Institut Pendidikan Guru Kampus Kota Bharu (IPG KKB). Instrumen kajian yang digunakan adalah berbentuk soal selidik adaptasi daripada Teori Fungsi Katz (1960) dan diubahsuai berdasarkan Volunteer Function Inventory (VFI). Analisis data dijalankan menggunakan perisian IBM SPSS Statistics (Statistical Packages for Social Sciences) Version 19.0 bagi mendapatkan data peratus, kekerapan, min dan sisihan piawai. Kajian rintis dijalankan dan nilai Alpha Cronbach yang diperolehi adalah 0.92. Dapatan kajian mendapati fungsi nilai (min= 4.32) dan fungsi pemahaman (min=4.31) merupakan faktor motivasi tertinggi yang menyumbang kepada penglibatan siswa guru dalam aktiviti sukarelawan membantu golongan OKU. Hasil kajian juga mendapati faktor jantina tidak begitu mempengaruhi motivasi penglibatan siswa guru IPG KKB dalam aktiviti sukarelawan membantu OKU. Namun begitu, terdapat perbezaan yang signifikan antara siswa guru lelaki dan siswa guru perempuan bagi penglibatan yang bermotivasikan peningkatan (t=3.14, p<0.05), perlindungan (t=4.14, p<0.05) dan kerjaya (t=2.88, p<0.05). Dapatan kajian memberi implikasi terhadap keperluan perancangan program sukarelawan dan khidmat masyrakat terhadap golongan OKU oleh pihak pentadbir IPGM bagi memupuk semangat kesukarelawan yang positif dalam kalangan siswa guru. Research on the identification of the involvement and motivation of pre service teachers at the Malaysian Teacher Education Institute (MTEI) with volunteerism activities for the Disabled is needed to provide basic information to MTEI administrators in designing volunteer programs to encourage pre service teachers to contribute in the community service activities especially to the disability group. This descriptive study aimed to identify the motivation of pre-service teachers’ involvement in volunteering activities to help the disabled and 70 respondents of pre-service teachers from the Kota Bharu Campus Teacher Education Institute (KBCTEI) were involved in this study. The instrument used was the adaptation questionnaire from the Katz Function Theory (1960) and was modified based on the Volunteer Function Inventory (VFI). Data analysis was performed using IBM SPSS Statistics (Statistical Packages for Social Sciences) Version 19.0 software for Windows to obtain standardized data of percentages, frequency, mean and standard deviation. A pilot study was conducted and found that the Alpha Cronbach's alpha was 0.92. The study found that the value function (mean=4.32) and comprehension function (mean=4.31) were the highest motivating factors contributing to the involvement of pre-service teachers in volunteering to assist the disabled. The study also found that gender factors did not significantly influence the involvement of KBCTEI pre-service teachers in volunteering to assist the disabled. However, there was a significant difference between male and female pre-service teachers for improving function (t=3.14, p<0.05), protection function (t=4.14, p<0.05) and career (t=2.88, p<0.05). The findings of the study provide implications for the needs of volunteer program planning and services for people with disabilities by MITE administrators to foster a positive volunteer spirit among pre-service teachers.

The first description of a disease resembling dengue fever (DF) was in the 15th century slave trade era by Spanish sailors visiting the Tanzania coast. The disease, then associated with evil spirits is now known to be caused by four serotypes of dengue virus (DENV1-4) that are transmitted by Aedes mosquitoes. Kenya has experienced multiple outbreaks, mostly associated with DENV-2. In this study, plasma samples obtained from 37 febrile patients during a DF outbreak at Kenya’s south coast in March 2019 were screened for DENV. Total RNA was extracted and screened for the alpha- and flavi-viruses by real-time polymerase chain reaction (qPCR). DENV-3 was the only virus detected. Shotgun metagenomics and targeted sequencing were used to obtain DENV whole genomes and the complete envelope genes (E gene) respectively. Sequences were used to infer phylogenies and time-scaled genealogies. Following Maximum likelihood and Bayesian phylogenetic analysis, two DENV-3 genotypes (III, n = 15 and V, n = 2) were found. We determined that the two genotypes had been in circulation since 2015, and that both had been introduced independently. Genotype III’s origin was estimated to have been from Pakistan. Although the origin of genotype V could not be ascertained due to rarity of these sequences globally, it was most related to a 2006 Brazilian isolate. Unlike genotype III that has been described in East and West Africa multiple times, this was the second description of genotype V in Kenya. Of note, there was marked amino acid variances in the E gene between study samples and the Thailand DENV-3 strain used in the approved Dengvaxia vaccine. It remains to be seen whether these variances negatively impact the efficacy of the Dengvaxia or future vaccines.

This paper discusses three issues that challenge contemporaneous taxonomy, with examples from the fields of mycology and lichenology, formulated as three questions: (1) What is the importance of taxonomy in contemporaneous and future science and society? (2) An increasing methodological gap in alpha taxonomy: challenge or opportunity? (3) The Nagoya Protocol: improvement or impediment to the science of taxonomy? The importance of taxonomy in society is illustrated using the example of popular field guides and digital media, a billion-dollar business, arguing that the desire to name species is an intrinsic feature of the cognitive component of nature connectedness of humans. While continuous societal support of a critical mass of taxonomists is necessary to catalogue all species on Earth, it is shown that this is a finite task, and a proposal is made how a remaining 10 million species can be catalogued within 40 years by 1,000 well-trained and dedicated taxonomists, with an investment of $4 billion, corresponding to 0.0001% of the annual global GDP or 0.005% of annual military expenditures. Notorious undercitation of actually used taxonomic resources and lack of coverage of impact metrics for monographs and other taxonomic work that cannot be published in indexed journals is discussed and suggestions are made how this problem can be remedied. An increasing methodological gap in approaches to taxonomy, between classic morphological and advanced genomic studies, affects in particular taxonomists in biodiversity-rich countries and amateurs, also regarding proper training to apply advanced methods and concepts. To counterbalance this problem, international collaborations bringing different expertise to the table and undertaking mutual capacitation are one successful remedy. Classic taxonomy still works for many groups and is a first approach to catalogue species and establish taxon hypotheses, but ultimately each taxonomic group needs to be studied with the array of methods proper to the group, including descriptive work. Finally, the Convention on Biological Diversity (CBD) and the Nagoya Protocol has put additional burden on basic biodiversity science. Using lichenology in Latin America and Brazil as an example, it is shown that the spirit of non-monetary benefit-sharing proper to taxonomy and systematics, namely capacitation, joint publications, and shared reference collections, has been increasingly implemented long before the CBD and the Nagoya Protocol, and does not need additional “policing”. Indeed, the Nagoya Protocol puts the heaviest burden on taxonomy and researchers cataloguing biodiversity, whereas for the intended target group, namely those seeking revenue gain from nature, the protocol may not actually work effectively. The notion of currently freely accessible digital sequence information (DSI) to become subject to the protocol, even after previous publication, is misguided and conflicts with the guidelines for ethical scientific conduct. Through its implementation of the Nagoya Protocol, Colombia has set a welcome precedence how to exempt taxonomic and systematic research from “access to genetic resources”, and hopefully other biodiversity-rich countries will follow this example.

AbstractAt present, bulk liquid water on the surface and near-subsurface of Mars does not exist due to the scarcity of condensed- and gas-phase water, pressure and temperature constraints. Given that the nuclei of soil and ice, that is, the soil solid and ice lattice, respectively, are coated with adsorbed and/or thin liquid films of water well below 273 K and the availability of water limits biological activity, we quantify lower and upper limits for the thickness of such adsorbed/water films on the surface of the Martian regolith and for subsurface ice. These limits were calculated based on experimental and theoretical data for pure water ice and water ice containing impurities, where water ice containing impurities exhibit thin liquid film enhancements, ranging from 3 to 90. Close to the cold limit of water stability (i.e. 273 K), thin liquid film thicknesses at the surface of the Martian regolith is 0.06 nm (pure water ice) and ranges from 0.2 to 5 nm (water ice with impurities). An adsorbed water layer of 0.06 nm implies a dessicated surface as the thickness of one monolayer of water is 0.3 nm but represents 0.001–0.02% of the Martian atmospheric water vapour inventory. Taking into account the specific surface area (SSA) of surface-soil (i.e. top 1 mm of regolith and 0.06 nm adsorbed water layer), shows Martian surface-soil may contain interfacial water that represents 6–66% of the upper- and lower-limit atmospheric water vapour inventory and almost four times and 33%, the lower- and upper-limit Martian atmospheric water vapour inventory. Similarly, taking the SSA of Martian soil, the top 1 mm or regolith at 5 nm thin liquid water thickness, yields 1.10×1013and 6.50×1013litres of waters, respectively, 55–325 times larger than Mars’ atmospheric water vapour inventory. Film thicknesses of 0.2 and 5 nm represent 2.3×104–1.5×106litres of water, which is 6.0×10−7–4.0×10−4%, respectively, of a 10 prμm water vapour column, and 3.0×10−6–4.0×10−4% and 6.0×10−6–8.0×10−4%, respectively, of the Martian atmospheric water vapour inventory. Thin liquid film thicknesses on/in subsurface ice were investigated via two scenarios: (i) under the idealistic case where it is assumed that the diurnal thermal wave is equal to the temperature of ice tens of centimetres below the surface, allowing for such ice to experience temperatures close to 273 K and (ii) under the, likely, realistic scenario where the diurnal thermal wave allows for the maximum subsurface ice temperature of 235 K at 1 m depth between 30°N and 30°S. Scenario 1 yields thin liquid film thicknesses ranging from 11 to 90 nm; these amounts represent 4×106–3.0×107litres of water. For pure water ice, Scenario 2 reveals that the thickness of thin liquid films contained on/within Martian subsurface is less than 1.2 nm, several molecular layers thick. Conversely, via the effect of impurities at 235 K allows for a thin liquid film thickness on/within subsurface ice of 0.5 nm, corresponding to 6.0×104litres of water. The existence of thin films on Mars is supported by data from the Mars Exploration Rovers (MERs) Spirit and Opportunity's Alpha Proton X-ray Spectrometer instrumentation, which have detected increased levels of bromine beneath the immediate surface, suggestive of the mobilization of soluble salts by thin films of liquid water towards local cold traps. These findings show that biological activity on the Martian surface and subsurface is not limited by nanometre dimensions of available water.

Abstract Background: Combination of Pegylated-Interferon alpha (Peg-IFNa) 2a and imatinib (IM) has been reported to significantly induce higher rates of molecular responses (including undetectable BCR-ABL transcript) over IM alone, as frontline therapy for CP-CML patients (pts) in a randomized phase 3 trial (SPIRIT, Preudhomme et al, NEJM 2010). Second generation TKIs such as dasatinib (DASISION, Kantarjian et al, NEJM 2010) enhance the speed and depth of molecular response (MR) in comparison to IM. Phase II trial using nilotinib and PegIFNa2a has recently reported high rates of deep molecular response (MR4.5) within 24 months (Nicolini FE et al, Lancet Haematology 2015). Aims: To determine the efficacy and safety of the combination of dasatinib and Peg-IFNa2b in CP-CML frontline. (EUDRACT Number: 2012-003389-42, Dasa-PegIFN trial). Methods: Newly diagnosed Ph+ CP-CML pts less than 65-year-old started dasatinib 100 mg/day. At 3 months, they were assigned to receive Peg-IFNa2b associated to dasatinib when platelets (plt) > 100 X 109/L, Neutrophils (ANC) > 1.5 X 109/L) and lymphocytes < 4.0 X 109/L counts were achieved. Otherwise, dasatinib was continued alone in the study according to the current international ELN guidelines. The maximum duration of the combination dasatinib and Peg-IFNa2b is 21 months. The primary endpoint is the cumulative rate of Molecular Response 4.5log (MR4.5 defined as BCR-ABL1/ABL1IS≤0.0032%) at 12 months. Molecular analyses were centralized and expressed according to the international scale (IS). Secondary endpoints included efficacy (cytogenetic and molecular responses at several time-points) and safety endpoints. Preliminary results are reported here. Results: 81 pts were enrolled between October 2013 and July 2014. All pts will have completed the 12 months follow-up time-point in August 2015. 79/81pts were included in the analysis (1 pt died of a CML-related haemorrhage before receiving dasatinib, 1 screening failure (masked Ph)). Median age was 48 (20-65) years. 54% of pts were male. Sokal scores were low, intermediate and high in 51%, 32% and 17% of pts respectively. After the first 3 months of therapy (M3), sixty-one patients (77%) started Peg-IFNa2b at the dose of 30 microg/week in association with dasatinib. For these pts after M3, reported hematologic adverse events (AE) were neutropenia (G3/4 n=11; G1/2 n=17), thrombocytopenia (G3/4 n=0; G1/2 n=7), anemia (G3/4 n=0; G1/2 n=7). Extra-hematologic AE were essentially of low grade (overall, G3/4 n=3; G1/2 n=113). According to NCI CTCAE V4.0, most frequent AE were infections (16%), general symptoms (15%), skin lesions (10%), hepato-biliary abnormalities (7.7%), nervous system/headache (7.7%) musculoskeletal pain (7%), psychiatric (7%), GI (6%) disorders. Eight serious AE (SAE) were reported after Peg-IFNa2b initiation: G4 neutropenia n=2, dysthyroitidis n=1, dyspnea n=1, pleural effusion n=1, lymphoid hyperplasia n=1, hemorrhoids n=1, rectal fistula (SUSAR) n=1. Efficacy was analysed according to the intention-to-treat principle (ITT), and considering missing data as no response to avoid inflated results. Overall at M3, 85% of pts had a BCR-ABL1/ABL1 ratio ≤10%. For eligible patients who received combined therapy (n=61), rates of MMR were 16%, 51%, 70%, and 70% (pending n=5) at M3, M6, M9 and M12, including MR4.5 rates 10%, 20%, 30% at M6, M9 and M12 respectively. Eighteen pts (22.7%) were not eligible to receive Peg-IFNa2b. Reasons, according to protocol criteria, were ANC <1.5 X 109/L (n=10), plt <100.0 X 109/L (n=5), lymphocytes >4.0 X 109/L (n=1), absence of complete hematologic response (n=1), non compliance (n=1). Rates of MMR for these pts were 27% at M6, 50 % at M9 (missing n=2), pending data for n=6 at M12. Conclusion: Peg-IFNa2b combined to dasatinib therapy in first line CP-CML induces a high rate of deep molecular response (ie MR4.5) during the first year of therapy. Despite few pending data, results at 12 months are already in line with previous data combining Peg-IFNa and TKI, expecting a potential for an increased rate in therapy cessation attempt. Preliminary data of this phase II trial indicate a manageable toxicity profile for this combination, despite an increased rate of neutropenia. Updated analyses (ITT and per protocol) will be presented for all the pts with at least 12 months follow-up. Disclosures ROY: BMS: Other: CongressTravels/Accomodations, Research Funding, Speakers Bureau; Novartis: Other: Congress Travels/Accomodations, Research Funding, Speakers Bureau; Merck: Other: Peg-Interferon provided in the trial. Guerci-Bresler:Novartis: Speakers Bureau; BMS: Speakers Bureau; ARIAD: Speakers Bureau; PFIZER: Speakers Bureau. Giraudier:BMS: Speakers Bureau; Novartis: Other: Congress Travel/Accomodation, Speakers Bureau. Johnson-Ansah:Hybrigenics SA: Consultancy, Research Funding; Novartis: Consultancy, Speakers Bureau; BMS: Speakers Bureau. Amé:Novartis: Speakers Bureau; BMS: Speakers Bureau. Etienne:BMS: Consultancy, Honoraria, Speakers Bureau; ARIAD: Consultancy, Honoraria, Speakers Bureau; Novartis: Consultancy, Honoraria, Other: Congress Travel/Accomodations, Research Funding, Speakers Bureau. Nicolini:BMS: Other: Travel/Accommodations/Expenses; Novartis: Other: Travel, Accommodations, Expenses; ARIAD: Honoraria, Research Funding, Speakers Bureau; BMS: Honoraria, Membership on an entity's Board of Directors or advisory committees, Other: Consulting or Advisory Role, Speakers Bureau; Novartis: Honoraria, Other: Consulting & Advisory Role, Research Funding, Speakers Bureau. Rea:Novartis: Honoraria; BMS: Honoraria; Ariad: Honoraria; Pfizer: Honoraria. Cony-Makhoul:Novartis: Consultancy, Honoraria, Speakers Bureau; BMS: Consultancy, Honoraria, Speakers Bureau. Ianotto:Novartis: Other: Congress Travel/ Accomodations. Legros:ARIAD: Speakers Bureau; Novartis: Research Funding, Speakers Bureau; BMS: Speakers Bureau. Coiteux:BMS: Speakers Bureau. Hermet:BMS: Speakers Bureau; Novartis: Speakers Bureau. Mahon:BMS: Speakers Bureau; Novartis: Speakers Bureau. Rousselot:ARIAD: Consultancy, Speakers Bureau; BMS: Consultancy, Speakers Bureau; Pfizer: Consultancy, Speakers Bureau; Novartis: Speakers Bureau.

Background and Objectives The aim of this study was to investigate the correlation between creativity and entrepreneurial skills in health system managers of the educational hospitals. Material and Methods This research is descriptive-analytical and applied in terms of purpose. The study population includes the managers of teaching hospitals in Ahvaz, south west Iran. The data was collected by census method from 110 senior and middle managers by validated questionnaire. Data were analyzed using absolute and relative frequency, mean, standard deviation, independent samples t-test, ANOVA, Pearson correlation coefficient and stepwise linear regression in SPSS software version 22. Results The mean score of creativity for managers was 44.74 7.91. 64.6% of the managers were highly creative and 35.4% of them were considered relatively creative. The average score of entrepreneurial skills of managers was 3 0.67 and was estimated to be appropriate. The correlation between creativity and entrepreneurial skills was 0.645, which showed a positive and strong relationship between these two variables (P <0.001). Simple linear regression showed that managerial skills among entrepreneurial skills determine 44.4% of creativity (P <0.001). Conclusion It is suggested to strengthen the level of managerial skills among managers by training courses Extended Abstract Background and Objectives Since creativity is assumed to be the mainstay of the spirit of entrepreneurship, thereforeit is necessary due measures be taken to establish the degree of creativity of health managers if one is to have any reasonable hope of developing enterepreueship in healthcare organizations. One way to do so might be to assess health managers’ creativity and determine if there is a correlation between their creativity and entrepreurship. Therefore, this study aimed to investigate the correlation between creativity and entrepreneurship skills among managers of educational hospitals in Ahvaz. Material and Methods This research was descriptive-analytical and applied in terms of purpose. The present research population consisted of managers of educational hospitals in Ahvaz city and data were collected by census method from 110 senior (management, metrons, educational assistants of hospitals) and mid-level (Supervisors, Head Nurses, Service Officers, Heads of Finance and Administration, Laboratory and Radiology Officers) managers through a questionnaire. In this study, two questionnaires were used: the Creativity Questionnaire, developed by Dorabjee et al., and the Smith Entrepreneurship Skills Questionnaire. The former consists of 12 five-point Likert scale items indicating the extent of the organization's support for creativity while the latter consists of 16 questions summarized in four components with each component having four questions. The validity of the questionnaire was confirmed in accordance with professors’ and experts’ opinions. The reliability of the questionnaires was estimated using Cronbach's alpha coefficient, which was 0.83 and 0.88 for for creativity questionnaire and entrepreneurial skills questionnaire, respectively. Data were analyzed using absolute and relative frequency, mean, standard deviation, independent samples t-test, ANOVA, Pearson correlation coefficient and stepwise linear regression in SPSS software version 22. Results The mean score of creativity for managers was 44.74±7.91. 64.6% of managers were found to be very creative and 35.4% were relatively creative. The mean score of managers' entrepreneurship skills was 3±0.67 which was considered at an appropriate level. Pearson correlation coefficient was estimated -0.27 (P=0.017) between age and creativity and-0.31 (P=0.04) between work experience and creativity indicating a small size and negative correlation between the former variables and a moderate and negative correlation between the latter ones. Likewise, Pearson correlation coefficient between age and entrepreneurial skills was calculated -0.28 (P=0.01) pointing to a small size and negative correlation between these two variables. As for the correlation coefficient between work experience and entrepreneurial skills was -0.32 (P=0.005) showing that these two variables have a moderate negative correlation. However, creativity and entrepreneurial skills were found to be positively and strongly correlated (r= 0.645, P<0.001). Simple linear regression showed that managerial skills among entrepreneurial skills come to determine 44.4% of creativity (P<0.001). Conclusion Managers of Ahvaz teaching hospitals were found to possess the required level of creativity and entrepreneurial skills as measured by the research tools in the present study with the creativity component showing a strong correlation with entrepreneurial skills. Management skills might be the most important indicators of creativity among hospital managers, it is suggested the barriers to creativity in the organization be removed, a financial system be formulated to encourage entrepreneurs, support creativity and innovation in the organization by formulating the necessary policies and guidelines, as well as holding classes and training courses to strengthen management skills among managers. Also, given the negative correlation between age/work experience on the one hand, and creativy/entrepreneurial skills on the other, it seems reasonable that due measures had best be taken to pave the way for the younger generation taking on managerial responsibilities. Practical implications of research The findings of the present study seem to point toa strong and significant relationship between creativity and entrepreneurial skills. Therefore, appropriate training programs should be designed and implemented to increase the creativity of hospital managers. Also, due to the high level of creativity and entrepreneurial skills of the managers of educational hospitals in Ahvaz, the trustees should take action to provide the best possible conditions for benefiting from this capacity. Ethical considerations Observance of ethical instructions: This study is based on the protocols of the ethics committee in the research of Ahvaz Jundishapur University of Medical Sciences with the code of ethics number IR.AJUMS.REC.1399.269 and also the study was conducted with the consent of the statistical population of the study. Conflict of interest The authors state that there is no conflict of interest in the present study. Aknowledgment This research has been done with the financial support of the Student Research Committee of Ahvaz Jundishapur University of Medical Sciences with the number 99S27. The authors are grateful to all the directors who participated in this study.

Biosurfactants are natural substances produced by several bacterial and fungal organisms that are amphiphilic and are extracellular (a part of the cell membrane). Biosurfactants can reduce the stress between solids and liquids on the surface and at the end. Biosurfactants have several properties, i.e. they are stable, less harmful, as well as readily degradable, and extremely eco-friendly. Biosurfactants also have a wide range of industrial uses because they are a versatile category of chemical substances. The principal justification for conducting such research was the isolation of possible biosurfactants containing bacteria. Sampling was performed for the isolation of bacteria producing biosurfactants from different oil-polluted sites That is to say, experiment for emulsification, test for oil spreading, test for drop collapse, and measure for hemolysis. The capability to produce biosurfactants was seen in 22 different isolates from polluted sites B1, B2, and B3. Through different biochemical tests and Gram staining, it was identified that isolated bacterial strains are Pseudomonas spp and that is Pseudomonas aeruginosa. The procedure used as characterizing biosurfactants was the TLC plate’s procedure, by using TLC plates process yellow dots emerged after spraying on silica gel plates with an throne and ninhydrin reagents. These yellow spots confirmed the presence and production of rhamnolipid in the biosurfactant. Hence, it was concluded that identified strains in the study can be helpful in the heavy metals, pesticides, and hydrocarbons bio-degradation and bioremediation. These may also be used as biological control agents to protect plants from various pathogens, resulting in improved crop yields. Introduction Biosurfactants are natural substances produced by several bacterial and fungal organisms that are amphiphilic and are extracellular (a part of the cell membrane) (Chen et al., 2007; Ghayyomiet al., 2012). Main purpose of the bio-surfactantsgeneration or production is a consequence of financial availability (Van Dyke et al., 1993 It is reported that almost 50 percent of the world's surfactants are used because of the need for cleaning agents as well as the rate of growth grows every day (Deleu and Paquot, 2004). Appropriate use of bio-surfactants will control environmental emissions what these are the most dangerous, constantly rising gradually and disrupting the routine maintenance of life every day. Awareness campaign initiatives have been introduced and also increase for environmental laws, various innovative approaches need to be implemented and even the issue of pollution focused entirely. Developing appropriate advanced technologies to help clear up chemicals and toxins from the ecosystem, like hydrocarbons (both inorganic and organic). Studies on biosurfactants are being launched by scholars and researchers with significant health issues like adverse environmental effects, air contamination, environmental change, and waste management (Makkar and Cameotra, 2002 Biosurfactants contribute to expanded demand for such microbial products as alternatives to chemical surfactants (Benatet al., 2000). Microbes seem to have the capability to degrade contaminants, but their biodegradation is limited leading to hydrophobicity, low solubility in water, and inadequate bioavailability, of such pollutants (Patil, et al., 2012). GhayyomiJazeh, Mishraet. al (2001) those bacteria that produce biosurfactants were isolated from the site of petroleum spills and afterward, 160 strains and as well as 59 strains were able to produce biosurfactants have shown better performance in a test for hemolysis of blood, and 45 strains with positive findings within oil spread experiment were applied in the laboratory to isolate and segregate the media cultured Banat process (Rahman et al., 2002) These were observed and researched that biosurfactants of Pseudomonas aeruginosa spp are most likely to disrupt the bonding of hydrocarbons like nonadecane, octa, Hexa, and hepta, in marine Water contaminated with oil spills up To approximately 47%, 53%, 73% and 60%(Abrar et al., 2020). Current study concluded that the isolated strain having the ability to degrade hydrocarbon as well as the ability to degrade the heavy metal. The strain also can protect the plant from various diseases. The present research found that the isolated strain is capable of degrading hydrocarbon while also being capable of degrading the heavy metal. As well as the strain does have the capability to defend plants from different diseases. Material And Methods Area of Study The investigation was conducted at HazaraUniversity(HU) Microbiology Laboratory, MansehraPakistan. Assemblage of Samples Thehomestay area of the city Mansehra Pakistan which is named as a township, where oil spills arose, oil spills soil samples were obtained as well as sampling from various Mansehra automobile workshops were also done. Sterilized bags of polythene were being used to collect samples of the soil, after thatthe sample was taken towards the Hazara University (HU) Mansehra Microbiology Laboratory to examine and extract bacterial strains that could develop biosurfactants. The soil temperature at the time of sample selection was around 30 ° C. The pH was also verified by Galvano science companies at the time of selection by pH meter, and the pH being reported was 7. Preparation of Media 15 x 100 mm Petri dishes were being used to prepare the media. Agar plates were thoroughly cleaned with water from the tap and then carefully covered in aluminum foil following cleaning then placed within autoclave at 121°C for about 15 min at 15 psi for sterilization. The nutrient agar which contains 0.5% NaCl, 0.3% beef extract, 0.5% peptone, and 1.5% agar, in 500 ml of distilled water, 14 g of the nutrient agar media (Merck) were dissolved. The nutrient level used mainly for the production of non-fastidious species. Nutrient agar is widely known as it's capable of growing a variety of bacteria types and provides nutrients required for the growth of bacteria. Upon sufficient dissolution of such nutrient agar in distilled water, these were then sterilized by autoclaving for 15 min at 15 psi in the autoclave and held at 121 °C Upon autoclaving, pouring of the media was done in laminar flow hood, and then packed and placed for yet more use in a fridge at 4°C. 2.4 Preparation of serial dilution The bacteria are isolated using the serial dilution process. During this process, 10 test tubes were taken and distilled water (9ml) was added in each tube. After that tubes were put for 15 minutes in the autoclave machine at 121°C. After that 1gm of a crude oil sample from the soil was added in a test tube containing distilled water. Further, 1 ml of the solution was taken from the first test tube and poured to the adjoining tubes for the preparation dilution as under . Afterward, 10μl of the solution was pipetted from both the dilution of and shifted for spread culture techniques, then incubated the plates at 37°C for 48hrs. Biosurfactants extraction Firstly, in nutrient broth solution theculture of bacteria was added and inoculated with oil, the bacterial colony was then incubated at the temperature of 25°C in a shaking incubator just for 7 days. Incubation after seven days of trembling. Thebacterial Crop was then taken and centrifuged at 5000rpm at temperature 4°C for 20minutes. Following centrifugation, the supernatant was collected and then mixed in the equivalent amount in Methanol: Chloroform. White sediment was then retained and collected for further use . Bacterial Colonies Isolation 1 g of the soil polluted with oil was diluted serially up to 106 dilutions.10 μl of 104 and 106 dilutions for spread culture were transferred to the MSM agar plates and nutrient agar. The plates were then incubated at 37°C for 48hrs. Twenty-two morphologically separate colonies were separated for further specific examination just after the incubation and processed by using the technique of streak plate. Screening of Isolates’ Biosurfactants Behavior To check the activity of biosurfactants produced by the bacterial species the following methods of screening were done. Hemolytic Activity of Biosurfactants for Erythrocytes Blood agar containing 5% of blood was prepared as after the fresh isolates were added and inoculated on blood agar plates, then the plates were taken and placed in the incubator at temperature 37°C for 48hrs (Rashediet al., 2005). Thereafter the observation of clear zone in the colonies indicated the existence of bacterial species that produce biosurfactants. This experiment was undertaken to control the ability of isolated bacteria to induce blood agar hemolysis. Three forms of hemolysis usually involve; alpha, beta, and hemolysis of the gamma. The agar underneath the species is dark greenish, then it is Alpha, the yellowish color produced in beta hemolysis and gamma hemolysis does not affect the bacterial sppwhichadded on the plates (Anandaraj and Thivakaran, 2010). Bio-surfactant identification with process of CTAB MSM (Mineral salt agar medium) with (2%) of glucose serving both as carbon source, (0.5 mg / ml) acetyl-tri-methyl-ammonium-bromide (CTAB), and methylene blue (MB: 0.2 mg/ml) are used to detect anionic bio-surfactants (Satpute et al., 2008). For this method, thirty microliters (30μl) of cell-free supernatant were added to each of the wells of the methylene blue agar plate that comprises of borer (4 mm in diameter). after that, the incubation of the plates was done for 48-72 hrs at 37°C. Just after incubation in each of the wells, a dark blue halo zone was being used to show the successful anionic bio-surfactant production. Table 1: Composition of MSM Media S. No Ingredients Amount (gm/L) I Potassium dihydrogen phosphate (KH2PO4) II Magnesium Sulfate (MgSO4) III Iron Sulfate (FeSO4) IV Sodium Nitrate (NaNO3) V Calcium Chloride (CaCl2) VI Ammonium Sulfate (NH4)2SO4 Technique for Spreading of Oil A sufficient number of isolated bacteria were inoculated into a solution of 100ml nutrient broth. Over 3 days, the culture was incubated at 37 ° C in a rotating shaker incubator (150 rpm). After that biosurfactants synthesis was checked in culture suspensions (Priya and Usharani, 2009; Anandaraj and Thivakaran, 2010). For this process, thirty milliliters (30ml) of distilled water was added in a Petri dish. In the middle of the distilled water, 1 milliliter (1ml) of diesel oil was added, and then a centrifuged twenty microliter (20μl) culture was introduced to the middle of a plate, which was isolated from oil spilled soil or local oily groundwater. The species producing the bio-surfactant displace the hydrocarbons and disperse it even in the water. Then it was calculated and analyzed within 1 mint (Ali et al., 2013). Technique for Drop collapse In this process, 96-wellsformed in each of the plates of nutrient agar. Afterwards, all the 96-wells of microliter plates was then filled withmineral oil of about 2ml. Then stabilized the plate at 37oC for 1 hour, after which the oil surface was filled with 5μl of supernatant culture. Therefore, the drop shape was taken to be observed on the oil surface after 1min. The drop which was collapsed, generated by the supernatant culture which is used to signify positive(+ive) outcome and the drops which stayed the same and displayed no changeindicates negative(-ive) outcome. And was taking distilled water as a control(Plaza et al., 2006). Emulsification index The emulsification index was calculated, as stated by the process followed by Cooper and Goldenberg (1981) In this process, 2 ml of kerosene oil was taken and inserted in each of the test tubes to the same amount of cell-free supernatant, and then homogenized for 2 min in a vortex at high speed and allowed for 24 hours to stand. The emulsification steadiness was then determined after the 24 hours, and the emulsification value was estimated by measuring the emulsified layer height by the total liquid layer height, then multiplied by 100. Quantification for the Dry weight of Biosurfactants The bacterial colony was inserted and inoculated in the nutrient broth medium, followed by oil and centrifuged at 5000rpm and after that, the supernatant was clutched and treated with chloroform and methanol and mixed. The white colored deposits were taken and used for the furtherprocess of dry weight. Afterwards, took the clean Petri plate and determined the empty plate weight. Next, the sediment was poured onto Petri plates. Now, for the drying process the hot air oven was used and set the 100ºC of temperature for 30minutes and the plates were put in the oven. After the drying process, the plates were weighted again. The dry weight was calculated for the biosurfactants using the formula which described below: Selected strains Identification and their characterization Instead, various basic biochemical methods were used to identify the isolated bacterial strains. Various biochemical tests, such as Gram staining, Oxidase test, Urease test.Catalase test, Methyl red test, Motility test, Indole test, Starch hydrolysis, Citrate test, Spore staining, Gelatin hydrolysis. Then afterwards, for the preliminary characterization of the biosurfactant, the thin layer chromatography process was used. Physical characterization of the strains selected Gram staining First, on the slide, using the wire loop the bacterial pure culture was taken, and smear was prepared on the slide, and then a drop of purified water was applied. Then, the sterile loop or needle was correctly mixed the bacterial colony and purified water, then mixed up until it is somewhat turbid. Then, spirit lamp was used to fixed the bacterial smear on slide and cooled to room temperature. With this glass slide was loaded with solution of crystal violet and stood for 1minute anddistilled water was applied on slide. Meanwhile the slide was submerged for 1 minute with the iodine solution, and then flushed and rinsed with water. Therefore, decolorizer of about 1 to 2 drops(5 percent acetone and 95 percent alcohol) were added to the slide’s smear and stand for 30seconds, and then treated with water. After then slide was rinsed with safranin for 60seconds, and then treated with water anddry in air. Microscopic analysis was done with 100x objective lenses using emersion oil on smear. Cell morphology The isolates of the bacterial cell were gram stained on slides and then the slides were observed under the light microscope, showing the shape and color of the cells. Biochemical characterization of the selected strains Catalase test Aim of this study is to identify, evaluate and examine that, whether or not the microbes are capable of producing catalase enzymes, while catalase is a protective enzyme, i.e. catalase has the potential to protect against the lethal chemicals known as (H2O2). In this study a bacterial culture that was clarified overnight was used. This culture has been smeared on a glass slide, and 3 percent hydrogen peroxide (H2O2) has been applied and observed on smear. Effects have been observed for bubble formation. Citrate test This study was performed to check the amount or ingest the citrate as the carbon and energy supply for growth and metabolism. Medium containing bromothymol blue and sodium citrate as pH indicator, bacterial was introduced. Ammonium chloride is also present in this medium used as a nitrogen source. Results were noted with variations of color from green to blue. Urease test The capability of urease enzyme for degrading urea was calculated in this bacterial capacity test. Bacterial culture was taken and inoculated for 48 hours at 37 ° C in urease broth, and then color was observed. Methyl red test Through using the process known as mixed acid fermentation which is used to evaluate the bacteria's acid production. The bacterial culture was taken and introduced in the broth of MR-VP and then incubated for 3days at a temperature of 37°C. Two (2) to three (3) drops of Methyl red were added in the broth medium after the incubation period. The change in broth color was observed for final results after a few seconds. Indole test Through using the process to assess the bacteria 's capability to crash indole from tryptophane molecules. After the 24 hours of incubated, taken the fresh inoculum of bacteria and then inserted into the tryptone medium, 24 hours of incubation of about 30oC, 2ml of the tryptone broth medium was added into a sterile test tube. Kovac's reagent was taken to be added (few drops) in sterile test tube and stimulated for a few minutes, and variations of color were detected. Gelatin test It is the approach assess to figure out the use of enzymes known as gelatins from bacterial organisms that precipitate the gelatin. Fresh inoculum of bacteria was taken after 24 hours, and inserted into the media of gelatin agar. This was incubated for around 24 hours, so the temperature did not exceed 30 ° C. Media was observed after incubation time. Starch hydrolysis Several of the micro-organisms that use the starch as a carbon energysource. Therefore, this method has been used to assess whether or not bacteria may use starch as a source of carbon. The bacterial fresh inoculum was spread on the petri starch agar plates, and after that the plate was incubated for 24 hours andmaintained the temperature at 30 to 35 ° C, then gradually applying the supplements of iodine to the plates to flow the change, and then examining the plates. Preliminary characterization of the strains selected Experimental characterization of the bio-surfactant was performed by using the process of TLC (Anandaraj et al., 2010). On a silica gel plate, crude portion of the rudimentary bio-surfactant was separated using Methanol: Chloroform: water (CH3OH: CHCl3: H2O) in the ratio of as an eluent with a different color producing reagents. Ninhydrin reagent (0.5 g ninhydrin in 100ml anhydrous acetone) was used to find bio-surfactant lipopeptide as red spots and anthrone reagent (1 g anthrone in 5ml sulfuric acid combined with 95ml ethanol) as yellow spots to identify rhamnolipid bio-surfactant (Yin et al., 2008). Results and Discussion Isolation of bacteria At first, twenty-two (22) strains from a polluted soil sample were isolated from nutrient agar media.Mixed culture provided by these colonies, so they were taken and smeared on the plates of nutrient agar and then fresh inoculum was collected and stored at temperature of 4oC for the further analysis. Bio-surfactants (surface-active compounds)are formed by a variety of amphiphilic bacterial and fungal organisms that are extracellular (a part of the cellular membrane) (Chen et al., 2007). Screening of Isolated strains for biosurfactant producing colonies Different experiments were carried out to identify, isolate and screen bacteria that are capable of generating bio-surfactants and that is Oil spreading technique(OST), blood hemolysis test(BHT), CTAB test, Emulsification operation. There were twenty-two distinct isolates observed in the current research. And the B1, B2 and B3culture were taken and selected from the twenty-two (22) strains isolated from the polluted spot, which were found to produce biosurfactant. And the oil spreading technique showed promising results for these strains. And strain B2 showed a greater displacement of oil and this is 4 mm. Oil spreading method is quick and often easy to handle, and this technique requires no particular equipment, only a very small amount of sample is used. This approach can be applied when the production and quantity of biosurfactant is small (Plaza et al., 2006) and (Youssef et al., 2004) Only bacterial cultures have been allocated and screened for bacterial species that can generate or use biosurfactants. Just three (3) strainsamong them presented the best results.Those 3 strain,s (B1, B2 and B3) were selected as an additional analysis. Blood hemolysis test On the petri plates of blood agar, the . Isolated bacteriaof B1, B2 and B3 were taken andstreak at the temperature about 37°C for 48 hours. Strain B1 demonstrated β (Beta) hemolysis after the incubation cycle and B2 and B3strains demonstrated γ (Gamma) hemolysis. The B1 strain had an emulsification index of about 74 percent and that was very high as compared to 70 percent for B2 and about 53 percent for B3 respectively. Around the same time, B1 strain showed β (Beta) hemolysis and γ (Gamma) hemolysis was shown bystrains B2 and B3 on the platesof bloodagar. The β hemolysisshowed by the strain B1 in the blood agar test, and the strain B2 and B3 showed γ (Gamma) hemolysis. It is determined that 20 percent strains that are the bestproducer of rhamnolipid have not fully lysed the blood, because the ability of the producer strains capacity not be responsible for the hemolytic activity. According to many researchers, who have shown that this is not such an effective tool for biosurfactant detection due to many bioproducts that may also induce red blood cell lysis, that is not so sufficient to be the surface-active molecule (Youssef et al., 2004). (Rashedi and others, 2005). Table2 Blood Hemolysis Test CTAB agar plate test This test confirms the anionic biosurfactants development. After plate incubation at a temperature of 37 ° C for 72 hours, dark blue hollow zone was existedaround each of the B1 strains wells, which clearly indicated the positive (+ive) development of anionic Biofactant. In addition, the B1 and B2 strains showed positive (+ I ve) results and, in the CTAB analysis, the B3 strain was found to be negative (-ive). The growing microorganisms when secreted the anionic biosurfactants on the plates of CTAB (cetyl-tri-methyl-ammonium-bromide) and methylene blue, then as a result the dark blue-purple insoluble ion pairs formed on the plates. The halo zone around each of the colonies was developed that can recognize rhamnolipid production and that was dark blue in colour, and could correlate with production of rhamnolipid (Siegmund et al., 1991). As indicated in (Fig1) Fig1: B1 positive on CTAB agar plate Oil Spreading Technique The oil was displaced by B1, B2and B3 strains in this test strain and showed a zone that was so clear. The bacterial strains capable of developing biosurfactant were tested and separated from the sample of soil which was oil spilled and brought from the District of Mansehra, Pakistan and from automobile workshops of Mansehra. As shown in (Fig.2). Fig.2: Results of Oil Spreading by B1, B2 and B3Table 3;.Test for oil spreads Bacterial culture Formation of zone (mm) Readings B,1 B,2 B,3 Drop-collapse technique During this process the drop shape was observed at the oil surface. As seen in Fig 3, the collapsed drop was provided by the supernatant culture B1 , B2 and B3.. Emulsification index Emulsification stability was measured with the use of kerosene oilin this test, and then observed the results. Since this emulsification index was calculated by dividing the height of the emulsion layer by the total height of the liquid layer and then multiplying by 100, as shown in the formulation below. Emulsification index Emulsification stability was measured with the use of kerosene oilin this test, and then observed the results. Since this emulsification index was calculated by dividing the height of the emulsion layer by the total height of the liquid layer and then multiplying by 100, as shown in the formulation below. Fig 3: Result of Drop-collapse test Table 4: The activity of Biosurfactant emulsification Dry weight of bio-surfactants In this examination, white-colored sediment was collected. Then measured the weight of the sterile Petri plate which was empty in the first step. Then, the sediment was poured into plates. The plates were taken and weighted after 30 minutes of drying on a hot air oven, following the process of drying. The weight of biosurfactants (dry weight) was measured using the following formulations: Fig 4: Dry weight of biosurfactants Table: 5: Dry weight of the biosurfactants Bacterial Culture Weight of the plate (g) biosurfactant in The plate after drying (g) Dry weight of Biosurfactant (g) B,1 B,2 B,3 Identification of selected strains and their characterization Gram staining For structural applications, and stroke analysis gram staining method was used.(Fig.5) shows findings from the process of gram staining. Fig 5: Microscopic view of Gram staining Biochemical identification of bacterial strains and their characterization Specific biochemical studies were performed to identify the species for further recognition and characterization. The bio-surfactant producing microorganism was found to be Pseudomonas aeruginosa after conducting various characterizations and the biochemical tests(Eric Deziel et al., 1996), Which can be used to further analyze and study the industrial development of the biosurfactant. Rhamnolipid is also isolated and produced from the Pseudomonas aeruginosa species on the silica gel plate (Rashedi et al., 2005), a form of biosurfactants highly recommended for processes of bioremediation. All the findings collected from biochemical testing were labeled as Berge 's Manual and it revealed that the protected microorganism was (Pseudomonas aeruginosa). Results of biochemical test were tabulated in (Table.5) Table 6: Bacterial strain identification Tests B1 B2 B3 Gram staining Negative Negative Negative Oxidases Positive e Positive Positive Catalase Positive Positive Positive Indole Positive Negative Negative Citrate Positive Negative Negative Urease Negative Positive Negative Nitrate Positive Positive Positive Motility Positive Positive Positive Gelatin hydrolysis Positive Negative Negative Lactose Negative Positive Positive Methyl red Negative Positive Positive Voges Proskauer Negative Negative Negative Fig 6: Results of biochemical tests(A) Methyl red and Voges Proskauer tests (b) catalase tests (c) oxidase tests (d) indole tests (e) citrate tests (g) lactose tests (h) urease tests Preliminary bacterial strain’s characterization The plates showed yellow dots, when sprayed with anthrone reagent. It indicated the existence of biosurfactants of rhamnolipid in the organism on the plate of TLC as seen in theFig.7 Fig 7: Biosurfactant characterization by TLC Conclusion Biosurfactant development is exciting and perceptible across industries to clean up oil waste and pollutants, particularly in the ecosystem.Compared with chemical surfactants, the biosurfactants are less harmful. It plays an important role in defining the advantages and the importance of industrial applications. Therefore, it is not possible to disregard the growing role and importance of biosurfactants in environmental sustainability.Biosurfactant formulations which can be used for bacterial, fungal, and viral organisms as growth inhibitors. Such biosurfactant inhibition properties can make them components that are applicable to Numerous illnesses that are used as medicinal agents. Therefore it was decided that the described strain could be used as a potential source for heavy metal bioremediation pesticide and hydrocarbon polluted sites. And also used as shielding the plant from different pathogens, contributing to improved crop yields. There is no doubt that the biosurfactants are a multifunctional, advanced, versatile, long-lasting and updated type not only for the twenty-first century but beyond. Conflict of interest The authors declared that they have no conflict of interest and the paper presents their own work which does not been infringe any third-party rights, especially authorship of any part of the article is an original contribution, not published before and not being under consideration for publication elsewhere. References Ali, S.R.; Chowdhury, B.R.; Mondal, P. and Rajak, S. “Screening and characterization of biosurfactants producing microorganism from natural environment (Whey spilled soil)”. Nat. Sci. Res. 2013, 3(13), 34–64. 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Angela Davis I heard Angela was coming to Honolulu Black History Month people still struggling globally El Niño season Afghanistan, Pakistan Iraq, Iran, red alert people hurt in prison unconscious injustice still around. I heard Angela was coming to Honolulu a real event like Miles & his red trumpet Sweet Honey in the Rock even Maya Angelou representing conflict & transformations. People fear real events like indigenous struggles while all around the stock market bucking like an angry bull affirmative action slipping away ebonics a clever diversion while real events are shadowed. I heard we were connected Alabama Massachusetts California Hawaii mutual friends I dreamed she came again to Hawaii before her dreads we walked together on the beach at Kualoa. After the Marin County, George Jackson fiasco She came before when she still wore an Afro fitted jeans high black pumps talking revolutionising thought. I heard she would be visiting So folk over here be working real hard to raise the fees and share some soulful aloha. And so I hope the vine is pumping and Angela is coming to share a space in time. Mumia Abu Jamal Knight for Justice Knight of Justice, Political prisoner. We watch your tragic drama like waiting for a tsunami generated by the quake of your daring to challenge like a race of people pounding at the black cliffs on a group of volcanic islands in the Pacific. The spirit of the wind hoodoos the trees— Transcendent revenge. Citation reference for this article MLA Style Waddell Takara, Kathryn. "Freedom Poems." M/C Journal 9.4 (2006). echo date('d M. Y'); ?> <http://journal.media-culture.org.au/0609/7-waddelltakara.php>. APA Style Waddell Takara, K. (Sep. 2006) "Freedom Poems," M/C Journal, 9(4). Retrieved echo date('d M. Y'); ?> from <http://journal.media-culture.org.au/0609/7-waddelltakara.php>.