Dissertations / Theses on the topic 'Spike Lee'
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Doughty, Ruth. "Scoring a black aesthetic : music in the films of Spike Lee." Thesis, Keele University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.412510.
Full textMota, Jorge Manuel Guedes Silva. "Representações dos africano-americanos em Do the right thing de Spike Lee." Master's thesis, Instituições portuguesas -- -Universidade Aberta, 1999. http://dited.bn.pt:80/29222.
Full textGaines, Mikal J. "Beating Songs: Blues, Violence, and the Male Body in the Films of Spike Lee." W&M ScholarWorks, 2005. https://scholarworks.wm.edu/etd/1593092170.
Full textWarren, Naomi Irene. "The politics of representation : a rhetorical analysis of Spike Lee's films, 1986-1998 /." Digital version accessible at:, 1999. http://wwwlib.umi.com/cr/utexas/fullcit?p9959605.
Full textSougstad, Timothy J. "Iconoclastic tradition in American literature /." free to MU campus, to others for purchase, 2001. http://wwwlib.umi.com/cr/mo/fullcit?p3036857.
Full textRodrigues, Vladimir Miguel [UNESP]. "Malcolm X: entre o texto escrito e o visual." Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/99127.
Full textMalcolm X foi figura exponencial durante a luta pelos direitos civis da população afroamericana nos EUA nas décadas de 1950 e 1960. Seu polêmico discurso pela resistência violenta das populações negras contra o racismo branco marcou gerações naquele país. Esta dissertação de mestrado pretende analisar as representações desse personagem histórico na obra “Autobiografia de Malcolm X”, texto biográfico escrito pelo jornalista Alex Halley e sua transcodificação para o Cinema no filme “Malcolm X” do cineasta Spike Lee
Malcolm X was a remarkable historical character during the Civil Rights struggles in the USA in the 1950s and 1960s. His polemical speech in favor of black resistance against the white racism was fundamental to the next generations in the country. This study aims at analyzing Malcolm´s representations in Alex Halley´s biography – Malcolm X – and its transcodification to the film X, directed by Spike Lee
Young, Kelcei. "And the Stereotype Award Goes to...: A Comparative Analysis of Directors using African American Stereotypes in Film." Thesis, University of North Texas, 2019. https://digital.library.unt.edu/ark:/67531/metadc1609173/.
Full textLavi, Tali, and talilavi@netspace net au. "Tales of Ash: Phantom Bodies as Testimony in Artistic Representations of Terrorism." RMIT University. Creative Media, 2007. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080428.114445.
Full textMorris, Tracy Louise. "Investigation of thoracic spine kinematics in adult sports participants with chronic groin pain during a single leg drop landing task." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86314.
Full textENGLISH ABSTRACT: Chronic groin pain is widespread across many sporting disciplines. The aim of our research was to determine if there are kinematic differences of the thoracic spine in active sports people with chronic groin pain, compared with healthy controls. A cross-sectional descriptive design was followed. Participants were required to complete six single leg drop landings with each leg from a 20cm height. The study was done in the 3D Movement Analysis Laboratory at the University of Stellenbosch. Ten male participants with unilateral or bilateral chronic groin pain of more than 3 months duration and 10 asymptomatic males, matched for age and sports participation, were recruited. The main outcome measures were: thoracic spine angle at initial foot contact, maximum thoracic spine angle, range of movement (ROM) (difference between the minimum and maximum values) and thoracic spine angle at lowest vertical point of the pelvis. This was assessed in all 3 movement planes: the sagittal plane (X plane), the coronal plane (Y plane) and the transverse plane (Z plane). The results of our study showed that for the unilaterally affected groin pain group, the cases landed in significantly more thoracic flexion (P<0.001 with large effect size) and were in significantly more thoracic flexion still at the lowest point. Peak thoracic flexion was significantly more in the cases than the controls. (P<0.001 with medium effect size) The same was true for the bilaterally affected group when landing on the most painful side, although this was not statistically significant. There were no significant differences in the frontal or transverse planes. In the bilaterally painful group, axial rotation ROM was significantly reduced when landing on either leg (worst affected side: P=0.040 with medium effect size and least affected side: p=0.006 with large effect size). The same occurred in the unilaterally affected group, although this was not statistically significant. Our study suggests that, in participants with chronic groin pain, there is greater thoracic forward flexion away from neutral during landing and that total axial rotation ROM during landing is diminished.
AFRIKAANSE OPSOMMING: Kroniese liespyn kom dikwels en in verskeie sportsoorte voor. Die doel van ons studie was om te bepaal of daar kinematiese verskille van die torakale werwelkolom is in aktiewe sportmense met chroniese liespyn, in vergelyking met gesonde kontroles. ‘n Dwars-deursnit beskrywende studiemetode is gevolg, en uitgevoer in die 3D Beweging Analise Laboratorium, Universiteit van Stellenbosch. Deelnemers moes ses landings op een been doen, met elke been, vanaf 'n 20cm hoogte. Tien mans met eensydige of bilaterale chroniese liespyn vir langer as 3 maande, en 10 asimptomatiese mans (ooreenstemmende ouderdom en sport deelname) het deelgeneem. Die hoof uitkomste wat gemeet is, was torakale werwelkolom krommingshoek by aanvanklike voet-kontak, maksimum torakale werwelkolom krommingshoek, omvang van beweging (OVB) (verskil tussen die minimum en maksimum waardes) en torakale werwelkolom krommingshoek by die laagste punt van die bekken. Dit is beoordeel in al 3 beweging vlakke: die sagittale (X) vlak, die koronale/frontale (Y) vlak en die transversale (Z) vlak. Die resultate van die studie het getoon dat, in die eensydig-geaffekteerde liespyn groep, die deelnemers in beduidend meer torakale fleksie geland het(P < 0.001, met 'n groot effekgrootte), asook met aansienlik meer torakale fleksie by die laagste punt na landing. Piek torakale fleksie was aansienlik meer in die liespyn-gevalle as in die kontroles. (P < 0.001, met middelmatige effekgrootte ) Dieselfde het vir die bilateraalgeaffekteerde groep gegeld wanneer hulle op hul mees pynlike kant geland het, hoewel dit nie statisties beduidend was nie. Daar was geen betekenisvolle verskille in die frontale of transversale vlakke van beweging nie. In die bilateraal pynlike groep, was aksiale rotasie OVB aansienlik verminder wanneer die gevalle op hul pynlikste been óf op hul minder pynlike been geland het ( mees pynlike been : P = 0,040, met 'n middelmatige effekgrootte en minder pynlike been : p = 0,006, met 'n groot effekgrootte ). Dieselfde het in die eensydig-geaffekteerde groep gebeur, hoewel dit nie statisties beduidend was nie. Ons studie dui daarop dat, in deelnemers met chroniese liespyn, daar meer torokale fleksie weg van neutraal tydens landing is en dat die totale aksiale rotasie OVB tydens die landing verminder is, in vergelyking met die kontrolegroep.
Huff, Melissa Lee. "Coming of Age in Spite of the Contrast of Vagueness: Native Speaker and The House on Mango Street as Erziehungsroman." BYU ScholarsArchive, 2008. https://scholarsarchive.byu.edu/etd/1489.
Full textAbdulla, Lana. "Kulturkonflikter : En komparativ studie av synen på ras och klass i Spike Lees Do The Right Thing & Mike Leighs Hemligheter och lögner." Thesis, Högskolan i Halmstad, Sektionen för humaniora (HUM), 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-4727.
Full textNonclercq, Antoine. "New strategies of acquisition and processing of encephalographic biopotentials." Doctoral thesis, Universite Libre de Bruxelles, 2007. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210711.
Full textSince the biopotentials produced by the upper parts of the brain have an amplitude of about one microvolt, the measurements performed by an EEG are exposed to many risks.
Moreover, since the present tendency is measure those signals over periods of several hours, or even several days, human analysis of the recording becomes extremely long and difficult. The use of signal analysis techniques for the help of paroxysm detection with clinical interest within the electroencephalogram becomes therefore almost essential. However the performance of many automatic detection algorithms becomes significantly degraded by the presence of interference: the quality of the recordings is therefore fundamental.
This thesis explores the benefits that electronics and signal processing could bring to electroencephalography, aiming at improving the signal quality and semi-automating the data processing.
These two aspects are interdependent because the performance of any semi-automation of the data processing depends on the quality of the acquired signal. Special attention is focused on the interaction between these two goals and attaining the optimal hardware/software pair.
This thesis offers an overview of the medical electroencephalographic acquisition chain and also of its possible improvements.
The conclusions of this work may be extended to some other cases of biological signal amplification such as the electrocardiogram (ECG) and the electromyogram (EMG). Moreover, such a generalization would be easier, because their signals have a wider amplitude and are therefore more resistant toward interference.
Doctorat en sciences appliquées
info:eu-repo/semantics/nonPublished
Dare, Michael Robert. "Investigation of hip kinematics in adult sports participants during single leg drop landing with chronic groin pain." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86334.
Full textENGLISH ABSTRACT: Introduction-Groin injuries are among the top six most cited injuries in soccer and account for 10-18 per cent of all injuries reported in contact sport. Groin pain can result from a variety of pathologies, but according to literature, 63 per cent of groin pain is due to adductor pathology. Objective-The objective of this study was to explore if there are kinematic differences in the hip joint in sports participants with groin pain compared to matched healthy controls. Study design A cross sectional, descriptive study was conducted. Study setting-The study was conducted at the FNB -3D motion analysis laboratory at the University of Stellenbosch, South Africa. Outcome variables-The dependent variables included hip kinematics in the sagittal, frontal and transverse planes at foot strike, lowest vertical point of the pelvis and total range of hip motion during a single leg drop landing. Methodology-The study sample comprised 20 male club level soccer-and, rugby players, running and cycling participants between the ages of 18-55 years of age. Ten of the subjects had chronic groin pain and the other ten were healthy matched controls. An eight-camera Vicon system was used to analyse the kinematics of the hip joint during single leg drop landing. For the purpose of comparison, the data was analysed for participants with unilateral groin pain and matched controls (n=14) and participants with bilateral groin pain and controls (n=6). The full set of data was subdivided for analysis into three distinct sub-groups. Unilaterally injured groin cases (n=7) were matched with seven healthy controls for analysis. Bilaterally injured groin cases (n=3) were matched with three healthy controls. Results-Cases with unilateral groin pain at initial contact had significantly more abduction of the hip joint when compared to controls (p<0.05). The effect size of this difference was large (0.94). Cases with unilateral groin pain also demonstrated greater hip internal rotation while the controls had external rotation (p<0.05) during a drop landing activity. Bilaterally injured groin cases landed with significantly (p=?) greater ranges of hip flexion as well as in significantly (p=?) more hip abduction during a drop landing activity. They also demonstrated greater total range of motion in the frontal plan when compared to controls. Groin pain cases overall demonstrated greater ranges of motion and tended to land in more abduction compared to controls. Conclusion-This study found that during a single leg drop landing, sports participants with unilateral chronic groin pain landed with significantly greater hip abduction and exhibited larger total range of motion in the transverse plane, which may indicate impaired stability of the hip complex when compared to controls.
AFRIKAANSE OPSOMMING: Inleiding-Liesbeserings is een van die top ses mees prominente sokker beserings. Dit beloop 10-18 persent van alle beserings wat in kontaksport aangemeld word. Liespyn kan die gevolg wees van ‘n verskeidenheid patologië, maar volgens die literatuur is 63 persent van liespyn as gevolg adduktor patologie. Doelwitte-Die doelwit van hierdie studie was om ondersoek in te stel of daar enige kinematiese veranderinge in die heupgewrig is in spelers met liespyn in vergelyking met dieselfde vergelykbare spelers sonder liespyn. Studie Ontwerp-‘n Deursnit, beskrywende studie was onderneem. Studie Omgewing-Die studie was uitgevoer by die FNB-3D bewegingsanalise laboratorium van die Stellenbosch Universiteit, Suid-Afrika. Uitkomsveranderlikes-Die afhanklike veranderlikes het in gesluit die heup kinematika in die sagitale, frontale en transvers vlakke met voet kontak endie laagste vertikale punt van die pelvis sowel as die totale heup omvang van beweging gedurende een been landing. Metodologie-Die studie populasie het bestaan uit 20 manlike sokker- en, rugbyspelers, hardlopers en fietsryers tussen die ouderdomme van 18 en 55 jaar. Tien van die deelnemers het kroniese liespyn gehad en die ander tien in die gelyke gesonde groep was sonder liespyn. Die agt kamera Vicon sisteem was gebruik om die kinematika van die heupgewrig te analseer tydens een been landing. Vir die doel om ‘n vergelyking te kan maak, was die data geanaliseer van deelnemers met unilaterale liespyn en die vergelykende groep sonder liespyn (n=14) en deelnemers met bilaterale liespyn en hulle vergelykende groep sonder liespyn (n=6).. Die volledige stel data was onderverdeel in drie afsonderlike sub groepe. Vir die analiese was unilaterale liesbeserings (n=7) vergelyk met sewe deelnemers sonder liespyn in die kontrolegroep. Deelnemers met bilaterale liesbeserings (n=3) was vergelyk met drie in die kontrolegroep. Resultate-Die deelnemers met unilaterale liespyn het met eerste kontak beduidend meer abduksie van die heupgewrig gehad in vergelyking met die kontrolegroep (p<0.05). Die effek van hierdie verskil was groot (0.94). Die deelnemers met unilaterale liespyn het ook ‘n grooter interne rotasie getoon, terwyl die kontrole groep meer eksterne rotasie gedemonstreer het (p<0.05) met landing. Deelnemers met bilaterale liespyn het beduidend (p=?) meer heup fleksie en abduksie omvang van beweging tydens landing. Hulle het ook ‘n groter totale heup omvang van beweging in die frontale vlak gehad in vergelyking met die kontrolegroep. Deelnemers met liespyn het oor die algemeen ‘n grooter omvang van beweging getoon, en was geneig om met meer abduksie van die heup te land as die kontrolegroep. Gevolgtrekking-Die studie toon dat deelnemers met kroniese unilaterale liespyn, tydens een been landing, beduidende meerheup abduksie toon en dat die heup in die transverse vlak meer totale omvang van beweging gebruik wat kan dui op onstabiliteit in die heupkompleks in vergelyking met die kontrolegroep.
Kanike, Vanaja. "“Acid-spike” effect in spurs/tracks of the low/high linear energy transfer radiolysis of water : potential implications for radiobiology and nuclear industry." Mémoire, Université de Sherbrooke, 2016. http://hdl.handle.net/11143/9711.
Full textAbstract : Hydronium ions (H3O+) are formed within spurs or tracks of the low or high linear energy transfer (LET) radiolysis of pure, deaerated water at early times. The in situ radiolytic formation of H3O+ renders the spur and track regions temporarily more acid than the surrounding medium. Although experimental evidence for an acidic spur has already been reported, there is only fragmentary information on its magnitude and time dependence. In this work, spur or track H3O+ concentrations and the corresponding pH values are obtained from our calculated yields of H3O+ as a function of time, using Monte Carlo track chemistry simulations. We selected four impacting ions and we used two different spur and track models: 1) an isolated “spherical” spur model characteristic of low-LET radiation and 2) an axially homogeneous “cylindrical” track model for high-LET radiation. Very good agreement was found between our calculated time evolution of G(H3O+) in the radiolysis of pure, deaerated water by 300-MeV incident protons (which mimic 60Co gamma/fast electron irradiation) and the available experimental data at 25 °C. For all cases studied, an abrupt transient acid pH effect, which we call an “acid spike”, is observed during and shortly after the initial energy release. This acid-spike effect is virtually unexplored in water or in a cellular environment subject to the action of ionizing radiation, especially high-LET radiation. In this regard, this work raises a number of questions about the potential implications of this effect for radiobiology, some of which are briefly evoked. Our calculations were then extended to examine the effect of temperature from 25 to 350 °C on the yield of H3O+ ions that are formed in spurs of the low-LET radiolysis of water. The results showed an increasingly acidic spike response at higher temperatures. As many in-core processes in a water-cooled nuclear reactor critically depend on pH, the question here is whether these variations in acidity, even highly localized and transitory, contribute to material corrosion and damage.
Williams, Langston A. "Stay Woke." ScholarWorks@UNO, 2017. https://scholarworks.uno.edu/td/2439.
Full textBouyer, Benjamin. "Utilisation des bases de données médico-administratives pour la recherche clinique et épidémiologique en orthopédie." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS462.
Full textThe National Health Data System includes data on all health care activities in France (outpatient care, hospital stay and social benefits related to diseases), which should be associated with mortality (causes of deaths, ongoing) and disability data (in the middle term). Many studies have already shown the interest of the analysis of these data for research purposes, in various fields (epidemiological studies of pathologies or groups of patients, evaluation of health products or medico-economic studies). The application for orthopedic research purposes is fairly recent. This surgical discipline has specificities in terms of data that we have exposed in the first part of this work; especially with regard to medical devices. The knowledge of these data made it possible to carry out an assessment of the national burden caused by fractures in France. More than 562,000 events were identified in 2016 for 1% of the adult population and nearly 12,000 (2%) were followed by early deaths. The detailed analysis showed specificities by fracture site and by sex with a significant increase of incidence with age for all groups. The third part of this work is devoted to the analysis of thromboembolic risk after spinal surgery. Analysis of detailed individual data has shown an intermediate risk (close to 1% in the general population) largely modulated by factors related to patients and surgical procedures performed.The National System of Health Data offers as we show in this thesis vast opportunities for research in orthopedics. Preparatory works, particularly in the field of the reliability of measures of efficacity of treatments are still needed to answer certain questions
Adedjouma, Morayo. "Requirements Engineering Process according to Automotive Standards in a Model-driven Framework." Phd thesis, Université Paris Sud - Paris XI, 2012. http://tel.archives-ouvertes.fr/tel-00724470.
Full textKnutsson, Björn. "Lumbar spinal stenosis : Body mass index and the patient's perspective." Doctoral thesis, Uppsala universitet, Ortopedi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-264589.
Full textWu, Yi-Lin, and 吳奕霖. "African-American Identity and Alienation: A Case Study of Spike Lee 's Films." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/6y89r2.
Full text國立臺灣師範大學
圖文傳播學系
105
Black actors found it quite difficult to break through in early Hollywood films; they were often portrayed as foolish or uncivilized, taking on roles as gangsters, clowns or country bumpkins among others. Spike Lee’s movies focus mostly on African Americans, as he tries to convey the existential issues and alienation suffered by black people under the yoke of colonial oppression. This study explores the different forms of alienation experienced by black people in Lee’s films, as well as exploring Spike Lee's own personal alienation through the narrative of his films. By making an independent movie, a filmmaker can avoid content control by those funding the movie. Since this research aims to explore the director’s existence and alienation through his films, the study needed to be based on Spike Lee's independently produced films. The decision on which films to include was based on two sets of criteria: first, whether or not they were directed by Spike Lee, and second, whether or not they were independently produced. Then correlation between the films narrative content was considered; finally, three films, “Do the Right Thing,” “Malcolm X,” and “Red Hook Summer,” became the main objects of the research. This study uses narrative analysis to transform the films into textual narrative “instruments descriptifs” and then adopts a theoretical framework to interpret the narrative content. Screenshots from the films are added into the discussion as "instruments citationnels" to help readers understand the narrative content and arguments within. Also included is content from interviews with Spike Lee, historical information, and findings from relevant studies. These "instruments documentaires" help to further explain the study and make it more complete. The analytical framework is based on Frantz Fanon's research into post-colonialism, and it uses the existentialist and dialectical aspects of his theories to investigate existence and alienation within the black community. This study first explains the conscious behavior of the subject; and this forms a basis for explaining black people’s alienation as a result of colonialism, as well as labor alienation. The study then goes on to clarify Spike Lee’s own form of alienation. The conclusion is that Spike Lee has been long aware of his own alienation, but has chosen to continue in this state of alienation and to continue making movies, with the aim of helping moviegoers to consider how to pursue de-alienation and de-colonization.
Almeida, Joana Araci Rodrigues. "The Universal Language of Pop Culture, Spike Lee's films as narratives of counter-memory." Master's thesis, 2021. http://hdl.handle.net/10316/96986.
Full textOlhar os Estados Unidos e todos os mitos que a rodeiam e o seu impacto global sempre foi crucial para perceber um mundo que, apesar de todas as mudanças, insiste em velhos problemas, ou como o racismo e a ascensão de velhas narrativas extremistas e da supremacia branca continuam a ser problemas presentes. Dada a importância da chamada ‘pop culture’, ou cultura de massas, na disseminação desses mitos à escala global para puro entretenimento, mas também na discussão crítica desses mitos, escolhi três filmes do realizador Spike Lee, Malcolm X (1991), Blackkklansman (2018) e Da 5 Bloods (2020) como representativos dessa dinâmica. Embora associados à indústria de Hollywood, estas narrativas cinematográficas parecem-me ilustrar a forma como a cultura se presta a ser palco de perspetivas variadas, neste caso, acerca do lugar e identidade da comunidade Afro-Americana nos E.U.A. No primeiro capítulo, exponho o entendimento de ‘pop’ culture que guia a minha análise; não como cultura de massas, mas no sentido de cultura popular defendido pela Escola de Birmingham, que salienta o seu carácter de oposição às narrativas dominantes. Nos capítulos seguintes, procedo à análise de cada um dos filmes, com base na perspetiva de estudiosos da Critical Race Theory, que propõem a categoria ‘raça’ como construção social, e também o conceito de ‘contra-memória’, elaborado por George Lipsitz e que me permite explicar a função das representações cinematográficas em questão. A minha seleção dos filmes deveu-se à sua capacidade de revisitação das narrativas dominantes através da criação de contra-memórias. Tanto a nível temático, como pelas técnicas cinematográficas usadas por Lee, estes são três filmes que se envolvem com aspetos da História dos Estados Unidos e assim permitem uma melhor perceção da complexidade da sociedade norte-americana.
Looking at the United States and all the myths that surround it as well as their global impact has always been crucial for understanding a world that, despite all the changes, insists on old problems, that is to say, how racism and the rise of old extremist narratives and white supremacy are still present. Given the importance of so-called 'pop culture', or mass culture, in disseminating these myths on a global scale for sheer entertainment, but also in critically discussing them, I have chosen three films by director Spike Lee, Malcolm X (1991), Blackkklansman (2018), and Da 5 Bloods (2020) as representative of that dynamics. Although associated with the Hollywood industry, these cinematic narratives illustrate, in my view, how culture lends itself to be the stage for varied perspectives, in this case, about the place and identity of the African American community in the U.S.A. In the first chapter, I set out the understanding of ‘pop’ culture that guides my analysis; not as mass culture, but in the sense of popular culture advocated by the Birmingham School, which stresses its oppositional character to dominant narratives. In the following chapters, I analyze each of the films based on the perspectives of Critical Race Theory scholars, who propose the category ‘race’ as a social construction and George Lipsitz’s elaboration of the concept ‘counter-memory’, which allows me to explain the function of the cinematic representations in question. My selection of the films was due to their ability to revisit dominant narratives through the creation of counter-memories. Both thematically and through the cinematic techniques used by Lee, these are three films that engage with aspects of U.S. history and thus allow for a better perception of the complexity of U.S. society.
Metzler, Jessica Lhamon W. T. "Genuine spectacle sliding positionality in the works of Pauline E. Hopkins, Zora Neale Hurston, Langston Hughes, and Spike Lee /." Diss., 2006. http://etd.lib.fsu.edu/theses/available/etd-01192006-155938.
Full textAdvisor: W.T. Lhamon, Florida State University, College of Arts and Sciences, Dept. of English. Title and description from dissertation home page (viewed June 9, 2006). Document formatted into pages; contains vii, 67 pages. Includes bibliographical references.
""No Brothers on the Wall": Black Male Icons in Spike Lee's Do the Right Thing." Master's thesis, 2012. http://hdl.handle.net/2286/R.I.14874.
Full textDissertation/Thesis
M.A. History 2012
Tan, Chung-Yueh, and 譚中岳. "Vaccination against severe acute respiratory syndrome (SARS) with the linear array epitopes (LAE) of different peptide regions on SARS-CoV spike protein (I)." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/71368704791999136993.
Full text國立中正大學
分子生物研究所
93
In coronavirus, the spike proteins were known to locate on the surface of virus particle and to participate in the entrance of virus into cells. According to the previous reports, there was higher healing possibility within seven days after infection of SARS Co-V clinically. In order to increase the chance to be cured, we proposed to develop a fast and efficient diagnostics kit to detect SARS-CoV at early stage of infection. The LAE technique was used to generate DNA fragments encoding for repeated epitopes of spike protein fused with GST or PEIa proteins. These fusion proteins were purified with affinity chromatography and then used to immunized rabbits for producing antibodies. The ELISA assays showed the purified immunogens can induce antisera production and in the Western blot assays, the antisera can against each indicated epitope. From blockage test, an antiserum against epitope 8 was proved to block virus infection. In addition, other research also show that the monoclonal antibody against amino acid 130 to 150 of spike protein has neutralizing activity. Therefore, in order to obtain the stable antibodies for diagnosis or vaccination in the future, we prepared the monoclonal antibodies of epitope 1 and epitope 8. We obtained several hybridoma clones, but these clones could not produce the monoclonal antibodies specifically against epitope 1 and epitope 8.
Gingras, Guillaume. "Validité de construit d’un test d’évaluation de la stabilité lombo-pelvienne, le soulèvement actif de la jambe tendue (SAJT) version objective." Thèse, 2009. http://hdl.handle.net/1866/3745.
Full textIntroduction: The clinical assessment of the trunk muscles neuromuscular control is lacking in rehabilitation and in conditioning. The main goal of this study was to assess the intra and inter-rater reliability of a clinical test, the active straight leg raise (ASLR), on an active and healthy population. Method: Each subject (18 tennis players) was tested twice by 2 physiotherapists with the ASLR. Results: The intra-rater reliability turned out to be high to very high (ICC = 0.81 à 0.93) while the inter-rater reliability came out clinically unsustainable. Conclusion: To our knowledge, the ASLR is the first reliable clinical test to assess the core neuromuscular control based on muscular and kinetic compensation observations with active and healthy subjects. A more exhaustive training of the assessors would definitely improve the inter-rater reliability.