Relevant bibliographies by topics / Spermidina

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Spermidina'

Author: Grafiati
Published: 22 February 2023

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Journal articles on the topic "Spermidina"

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Lee, Jung Yeop, Brian K. Janes, Karla D. Passalacqua, Brian F. Pfleger, Nicholas H. Bergman, Haichuan Liu, Kristina Håkansson, et al. "Biosynthetic Analysis of the Petrobactin Siderophore Pathway from Bacillusanthracis." Journal of Bacteriology 189, no. 5 (December 22, 2006): 1698–710. http://dx.doi.org/10.1128/jb.01526-06.

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ABSTRACT The asbABCDEF gene cluster from Bacillus anthracis is responsible for biosynthesis of petrobactin, a catecholate siderophore that functions in both iron acquisition and virulence in a murine model of anthrax. We initiated studies to determine the biosynthetic details of petrobactin assembly based on mutational analysis of the asb operon, identification of accumulated intermediates, and addition of exogenous siderophores to asb mutant strains. As a starting point, in-frame deletions of each of the genes in the asb locus (asbABCDEF) were constructed. The individual mutations resulted in complete abrogation of petrobactin biosynthesis when strains were grown on iron-depleted medium. However, in vitro analysis showed that each asb mutant grew to a very limited extent as vegetative cells in iron-depleted medium. In contrast, none of the B. anthracis asb mutant strains were able to outgrow from spores under the same culture conditions. Provision of exogenous petrobactin was able to rescue the growth defect in each asb mutant strain. Taken together, these data provide compelling evidence that AsbA performs the penultimate step in the biosynthesis of petrobactin, involving condensation of 3,4-dihydroxybenzoyl spermidine with citrate to form 3,4-dihydroxybenzoyl spermidinyl citrate. As a final step, the data reveal that AsbB catalyzes condensation of a second molecule of 3,4-dihydroxybenzoyl spermidine with 3,4-dihydroxybenzoyl spermidinyl citrate to form the mature siderophore. This work sets the stage for detailed biochemical studies with this unique acyl carrier protein-dependent, nonribosomal peptide synthetase-independent biosynthetic system.
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Ponasik, J. A., C. Strickland, C. Faerman, S. Savvides, P. A. Karplus, and B. Ganem. "Kukoamine A and other hydrophobic acylpolyamines: potent and selective inhibitors of Crithidia fasciculata trypanothione reductase." Biochemical Journal 311, no. 2 (October 15, 1995): 371–75. http://dx.doi.org/10.1042/bj3110371.

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The enzyme trypanothione reductase (TR), together with its substrate, the glutathione-spermidine conjugate trypanothione, plays an essential role in protecting parasitic trypanosomatids against oxidative stress and is a target for drug design. Here we show that a naturally occurring spermine derivative, the antihypertensive agent kukoamine A [N1N12-bis(dihydrocaffeoyl)-spermine] inhibits TR as a mixed inhibitor (Ki = 1.8 microM, Kii = 13 microM). Kukoamine shows no significant inhibition of human glutathione reductase (Ki > 10 mM) and thus provides a novel selective drug lead. The corresponding N1N8-bis(dihydrocaffeoyl)spermidine derivative was synthesized and acted as a purely competitive inhibitor with Ki = 7.5 microM. A series of mono- and di-acylated spermines and spermidines were synthesized to gain an insight into the effect of polyamine chain length, the nature and position of the acyl substituent and the importance of conformational mobility. These compounds inhibited TR with Ki values ranging from 11 to 607 microM.
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Alves da Costa Ribeiro Quintans, Isadora Louise, Juliana Alves da Costa Ribeiro Souza, and Michael K. Deyholos. "Orbitides and free polyamines have similarly limited fungicidal activity against three common pathogens of flax in vitro." FACETS 7 (January 1, 2022): 843–52. http://dx.doi.org/10.1139/facets-2021-0201.

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Fusarium oxysporum f. sp. lini and Septoria linicola are causes of fusarium wilt and pasmo in flax ( Linum usitatissimum). Members of a third fungal genus, Alternaria spp., have also been found in fiber and linseed varieties of flax, and are a source of post-harvest spoilage and mycotoxins in a wide range of crops. We performed a microdilution assay and calculated the median effective concentration (EC50) to compare the potency of cyclolinopeptides (CLPs), two polyamines (spermidine and spermine), and the fungicide carbendazimin in the control of three fungi that have potential pathogenic activity ( F. oxysporum, S. linicola, and Alternaria spp), of which the first two are particulary significant causes of disease in flax. For carbendazim, all EC50 values were <0.6 μg/mL. The observed EC50 ranged from 111 to 340 μg/mL for a mixture of six unique CLPs, 109 to 778 μg/mL for spermine, and 21 to 272 μg/mL for spermidine. Spermidine was most effective against Alternaria sp., with an EC50 of 21 μg/mL. The results presented here showed that polyamines and CLPs possess limited antifungal activities against several fungi, with spermidines the most effective naturally occurring compound tested. Our findings do not support the hypothesis that CLPs act as potent antifungals against the three species of pathogens tested.
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Mercier, A. J., S. Farragher, B. Schmor, M. Kamau, and J. Atkinson. "Effect of a plant-derived spider toxin analogue on crayfish neuromuscular junctions." Canadian Journal of Zoology 76, no. 11 (November 1, 1998): 2103–7. http://dx.doi.org/10.1139/z98-142.

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N8-Coumaroyl spermidine (N8-CS) is an example of hydroxycinnamic acid - polyamine conjugates found in certain plants. Because of its structural similarity to some spider toxins, N8-CS was tested for its ability to block arthropod neuromuscular synapses. It inhibited chemical synaptic transmission at crayfish (Procambarus clarkii) neuromuscular junctions, the IC50 being approximately 200 µM. Its effect was at least partially reversed by washing with physiological saline. Joro spider toxin, a structurally similar compound, also blocked crayfish neuromuscular synapses but its effect was irreversible. These results suggest that plant-derived cinnamoyl spermidines might have paralytic properties similar to those of spider toxins.
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Perrin, Jennifer, Natalja Kulagina, Marianne Unlubayir, Thibaut Munsch, Inês Carqueijeiro, Thomas Dugé de Bernonville, Johan-Owen De Craene, et al. "Exploiting Spermidine N-Hydroxycinnamoyltransferase Diversity and Substrate Promiscuity to Produce Various Trihydroxycinnamoyl Spermidines and Analogues in Engineered Yeast." ACS Synthetic Biology 10, no. 2 (January 15, 2021): 286–96. http://dx.doi.org/10.1021/acssynbio.0c00391.

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Hamana, Koei, and Shigeru Matsuzaki. "Polyamine distribution patterns serve as a phenotypic marker in the chemotaxonomy of the Proteobacteria." Canadian Journal of Microbiology 39, no. 3 (March 1, 1993): 304–10. http://dx.doi.org/10.1139/m93-043.

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Polyamines of various genera of the class Proteobacteria were analyzed by high-performance liquid chromatography to determine if they can serve as taxonomic markers. The major polyamine of Zymomonas was homospermidine, whereas the Acetobacter–Gluconobacter complex contained spermidine, suggesting the presence of two different polyamine distribution patterns in the alpha subclass. Both the homospermidine-dominant type and the spermidine-dominant type were found in heterogeneous Sphingomonas species. Typical species belonging to the gamma subclass have their own unique polyamine pattern in Xanthomonas (spermidine), Azomonas (putrescine), Frateuria (spermidine), Alteromonas (putrescine–spermidine or spermidine), Shewanella (putrescine), Marinomonas (putrescine–spermidine or spermidine), Halomonas (putrescine–spermidine or spermidine), and Deleya (spermidine). Cadaverine was sporadically distributed in some species in these genera. Some strains classified into Rhizobacter, Zoogloea, Azomonas, or Alteromonas contained 2-hydroxyputrescine found exclusively in the beta subclass. Polyamine distribution patterns are genus- and (or) species-specific and can serve as a phenotypic marker in the chemotaxonomy of the Proteobacteria.Key words: polyamine, chemotaxonomy, Proteobacteria.
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Higashi, Kyohei, Hiroyuki Ishigure, Risa Demizu, Takeshi Uemura, Kunihiko Nishino, Akihito Yamaguchi, Keiko Kashiwagi, and Kazuei Igarashi. "Identification of a Spermidine Excretion Protein Complex (MdtJI) in Escherichia coli." Journal of Bacteriology 190, no. 3 (November 26, 2007): 872–78. http://dx.doi.org/10.1128/jb.01505-07.

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ABSTRACT A spermidine excretion protein in Escherichia coli was looked for among 33 putative drug exporters thus far identified. Cell toxicity and inhibition of growth due to overaccumulation of spermidine were examined in an E. coli strain deficient in spermidine acetyltransferase, an enzyme that metabolizes spermidine. Toxicity and inhibition of cell growth by spermidine were recovered in cells transformed with pUCmdtJI or pMWmdtJI, encoding MdtJ and MdtI, which belong to the small multidrug resistance family of drug exporters. Both mdtJ and mdtI are necessary for recovery from the toxicity of overaccumulated spermidine. It was also found that the level of mdtJI mRNA was increased by spermidine. The spermidine content in cells cultured in the presence of 2 mM spermidine was decreased, and excretion of spermidine from cells was enhanced by MdtJI, indicating that the MdtJI complex can catalyze excretion of spermidine from cells. It was found that Tyr4, Trp5, Glu15, Tyr45, Tyr61, and Glu82 in MdtJ and Glu5, Glu19, Asp60, Trp68, and Trp81 in MdtI are involved in the excretion activity of MdtJI.
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Elejalde-Palmett, Carolina, Thomas Dugé de Bernonville, Gaëlle Glevarec, Olivier Pichon, Nicolas Papon, Vincent Courdavault, Benoit St-Pierre, Nathalie Giglioli-Guivarc’h, Arnaud Lanoue, and Sébastien Besseau. "Characterization of a spermidine hydroxycinnamoyltransferase inMalus domesticahighlights the evolutionary conservation of trihydroxycinnamoyl spermidines in pollen coat of core Eudicotyledons." Journal of Experimental Botany 66, no. 22 (September 12, 2015): 7271–85. http://dx.doi.org/10.1093/jxb/erv423.

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Erwin, B. G., and A. E. Pegg. "Regulation of spermidine/spermine N1-acetyltransferase in L6 cells by polyamines and related compounds." Biochemical Journal 238, no. 2 (September 1, 1986): 581–87. http://dx.doi.org/10.1042/bj2380581.

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Exposure of rat L6 cells in culture to exogenous polyamines led to a very large increase in the activity of spermidine/spermine N1-acetyltransferase. Spermine was more potent than spermidine in bringing about this increase, but in both cases the elevated acetyltransferase activity increased the cellular conversion of spermidine into putrescine. The N1-acetyltransferase turned over very rapidly in the L6 cells, with a half-life of 9 min after spermidine and 18 min after spermine. A wide variety of synthetic polyamine analogues also brought about a substantial induction of spermidine/spermine N1-acetyltransferase activity. These included sym-norspermidine, sym-norspermine, sym-homospermidine, N4-substituted spermidine derivatives, 1,3,6-triaminohexane, 1,4,7-triaminoheptane and deoxyspergualin, which were comparable with spermidine in their potency, and N1N8-bis(ethyl)spermidine, N1N9-bis(ethyl)homospermidine, methylglyoxal bis(guanylhydrazone), ethylglyoxal bis(guanylhydrazone) and 1,1′-[(methylethanediylidene)dinitrilo]bis(3-amino-guanidine), which were even more active than spermidine. It is suggested that these polyamine analogues may bring about a decrease in cellular polyamines not only by inhibiting biosynthesis but by stimulating the degradation of spermidine into putrescine.
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Liao, Chen-Yu, Oona M. P. Kummert, Amanda M. Bair, Nora Alavi, Josef Alavi, Delana M. Miller, Isha Bagga, et al. "The Autophagy Inducer Spermidine Protects Against Metabolic Dysfunction During Overnutrition." Journals of Gerontology: Series A 76, no. 10 (June 1, 2021): 1714–25. http://dx.doi.org/10.1093/gerona/glab145.

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Abstract Autophagy, a process catabolizing intracellular components to maintain energy homeostasis, impacts aging and metabolism. Spermidine, a natural polyamine and autophagy activator, extends life span across a variety of species, including mice. In addition to protecting cardiac and liver tissue, spermidine also affects adipose tissue through unexplored mechanisms. Here, we examined spermidine in the links between autophagy and systemic metabolism. Consistently, daily injection of spermidine delivered even at late life is sufficient to cause a trend in life-span extension in wild-type mice. We further found that spermidine has minimal metabolic effects in young and old mice under normal nutrition. However, spermidine counteracts high-fat diet (HFD)-induced obesity by increasing lipolysis in visceral fat. Mechanistically, spermidine increases the hepatokine fibroblast growth factor 21 (FGF21) expression in liver without reducing food intake. Spermidine also modulates FGF21 in adipose tissues, elevating FGF21 expression in subcutaneous fat, but reducing it in visceral fat. Despite this, FGF21 is not required for spermidine action, since Fgf21−/− mice were still protected from HFD. Furthermore, the enhanced lipolysis by spermidine was also independent of autophagy in adipose tissue, given that adipose-specific autophagy-deficient (Beclin-1flox/+Fabp4-cre) mice remained spermidine-responsive under HFD. Our results suggest that the metabolic effects of spermidine occur through systemic changes in metabolism, involving multiple mechanistic pathways.
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Dissertations / Theses on the topic "Spermidina"

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Stöckle, Silke. "Thin liquid films with nanoparticles and rod-like ions as models for nanofluidics." Phd thesis, Universität Potsdam, 2010. http://opus.kobv.de/ubp/volltexte/2010/4637/.

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With the rise of nanotechnology in the last decade, nanofluidics has been established as a research field and gained increased interest in science and industry. Natural aqueous nanofluidic systems are very complex, there is often a predominance of liquid interfaces or the fluid contains charged or differently shaped colloids. The effects, promoted by these additives, are far from being completely understood and interesting questions arise with regards to the confinement of such complex fluidic systems. A systematic study of nanofluidic processes requires designing suitable experimental model nano – channels with required characteristics. The present work employed thin liquid films (TLFs) as experimental models. They have proven to be useful experimental tools because of their simple geometry, reproducible preparation, and controllable liquid interfaces. The thickness of the channels can be adjusted easily by the concentration of electrolyte in the film forming solution. This way, channel dimensions from 5 – 100 nm are possible, a high flexibility for an experimental system. TLFs have liquid IFs of different charge and properties and they offer the possibility to confine differently shaped ions and molecules to very small spaces, or to subject them to controlled forces. This makes the foam films a unique “device” available to obtain information about fluidic systems in nanometer dimensions. The main goal of this thesis was to study nanofluidic processes using TLFs as models, or tools, and to subtract information about natural systems plus deepen the understanding on physical chemical conditions. The presented work showed that foam films can be used as experimental models to understand the behavior of liquids in nano – sized confinement. In the first part of the thesis, we studied the process of thinning of thin liquid films stabilized with the non – ionic surfactant n – dodecyl – β – maltoside (β – C₁₂G₂) with primary interest in interfacial diffusion processes during the thinning process dependent on surfactant concentration 64. The surfactant concentration in the film forming solutions was varied at constant electrolyte (NaCl) concentration. The velocity of thinning was analyzed combining previously developed theoretical approaches. Qualitative information about the mobility of the surfactant molecules at the film surfaces was obtained. We found that above a certain limiting surfactant concentration the film surfaces were completely immobile and they behaved as non – deformable, which decelerated the thinning process. This follows the predictions for Reynolds flow of liquid between two non – deformable disks. In the second part of the thesis, we designed a TLF nanofluidic system containing rod – like multivalent ions and compared this system to films containing monovalent ions. We presented first results which recognized for the first time the existence of an additional attractive force in the foam films based on the electrostatic interaction between rod – like ions and oppositely charged surfaces. We may speculate that this is an ion bridging component of the disjoining pressure. The results show that for films prepared in presence of spermidine the transformation of the thicker CF to the thinnest NBF is more probable as films prepared with NaCl at similar conditions of electrostatic interaction. This effect is not a result of specific adsorption of any of the ions at the fluid surfaces and it does not lead to any changes in the equilibrium properties of the CF and NBF. Our hypothesis was proven using the trivalent ion Y3+ which does not show ion bridging. The experimental results are compared to theoretical predictions and a quantitative agreement on the system’s energy gain for the change from CF to NBF could be obtained. In the third part of the work, the behavior of nanoparticles in confinement was investigated with respect to their impact on the fluid flow velocity. The particles altered the flow velocity by an unexpected high amount, so that the resulting changes in the dynamic viscosity could not be explained by a realistic change of the fluid viscosity. Only aggregation, flocculation and plug formation can explain the experimental results. The particle systems in the presented thesis had a great impact on the film interfaces due to the stabilizer molecules present in the bulk solution. Finally, the location of the particles with respect to their lateral and vertical arrangement in the film was studied with advanced reflectivity and scattering methods. Neutron Reflectometry studies were performed to investigate the location of nanoparticles in the TLF perpendicular to the IF. For the first time, we study TLFs using grazing incidence small angle X – ray scattering (GISAXS), which is a technique sensitive to the lateral arrangement of particles in confined volumes. This work provides preliminary data on a lateral ordering of particles in the film.
Mit dem Heranwachsen der Nanotechnologie in den vergangenen zehn Jahren hat sich die Nanofluidik als Forschungsbereich etabliert und erfährt wachsende Aufmerksamkeit in der Wissenschaft, sowie auch in der Industrie. Im biomedizinischen Bereich, wo intrazelluläre Prozesse häufig komplexer, nanofluidischer Natur sind, wird sich vermehrt für ein detailliertes Verständnis von nanofluidischen Prozessen interessiert, z.B. für den Einfluss von Kolloiden verschiedenster Form oder elektrischer Ladung auf die Kanäle und auf das Fließverhalten oder die Auswirkungen der Einengung von Flüssigkeiten und Kolloiden in Nanometer Geometrien. In der vorliegenden Arbeit werden dünne flüssige Filme, hinsichtlich ihrer Funktion als nanofluidische Modelle untersucht. Im ersten Teil der Arbeit wurde die Fließgeschwindigkeit des Fluids aus dem dünnen Film, abhängig von der Konzentration der filmstabilisierenden Tensidmoleküle n – Dodecyl β – D – Maltoside ( β – C₁₂G₂) bei einer konstanten Elektrolytkonzentration von 0.2 mM NaCl untersucht. Mit einem theoretischen Modell konnte das Dünnungsverhalten nachgezeichnet werden. Es wurde eine kritische Tensidkonzentration gefunden, unter der die Oberflächen lateral mobil sind und über der sie sich wie fest verhalten. Dadurch konnten wir Aufschluss darüber erlangen, wie die Oberfläche des Films unter verschiedenen Bedingungen geschaffen ist, und das in Bezug zur Verteilungsdichte der Moleküle an den Oberflächen setzen. Im weiteren wurden komplexere, nanofluidische Systeme untersucht, wobei zum einen ~ 1 nm lange, stäbchenförmige, multivalent geladene Spermidin - Moleküle die punktförmigen Elektrolyte ersetzten. Es konnte eine deutliche Veränderung der Stabilität zwischen Filmen mit und ohne Stäbchen festgestellt werden. Die Filme, mit NaCl, blieben länger in dem metastabilen „Common Film“ (CF) Zustand als die Filme, die eine vergleichbare Konzentration von Spermidin Stäbchen beinhalteten. Die Ergebnisse deuteten auf eine zusätzliche Anziehungskraft durch Brückenbildung zwischen zwei geladenen Oberflächen durch gegensätzlich geladene Stäbchenförmige Moleküle hin. Es konnte gezeigt werden, dass dieser Effekt weder ein Ergebnis von spezifischer Ionenadsorption an die Filmoberfläche war, noch ein Unterschied in den Gleichgewichtszuständen von den Dicken der CFs und der Newton Black Films (NBFs) hervorrief, was auf die korrekte Annahme der Ionenstärke in der Lösung schließen ließ. Auch in Versuchen mit ebenfalls trivalenten Ionen YCl3 wurde festgestellt, dass kein vergleichbarer Überbrückungseffekt auftritt. Die Ergebnisse wurden mit theoretischen Simulationen verglichen und es wurde eine quantitative Übereinstimmung gefunden bezüglich der Größe des Systeminternen Energiegewinns durch den Überbrückungseffekt. Desweiteren wurde das Fließverhalten von Fluiden mit Kolloiden eingeengt in Nanometer Geometrien untersucht. Für zwei verschiedene Arten von Nanopartikeln (Fe3O4 stabilisiert mit Oleinsäure und polymerstabilisierte Goldpartikel) wurde eine Verlangsamung der Fließgeschwindigkeit festgestellt. Mit einem theoretischen Modell konnte das Fließverhalten nur für enorm erhöhte Viskositätswerte des Fluids erklärt werden. Die Viskositätserhöhung wurde mit Partikelaggregaten, die den Ausfluss behindern, erklärt und diskutiert, unter der Annahme eines nicht - Newtonischen Fließverhaltens der Dispersionen. Gleichermaßen wurde die strukturelle Anordnung der Partikel in den Filmen hinsichtlich ihrer vertikalen und lateralen Verteilung untersucht. In dieser Arbeit werden vorläufige Ergebnisse präsentiert, die noch weiteren Studien bedürfen. Mit Neutronenreflexion sollte die Anordnung der Partikel orthogonal zur Oberfläche im Film analysiert werden. Eine qualitative Analyse lässt schließen, dass bei einer höheren Konzentration von Partikeln in Lösung, sich auch eine erhöhte Konzentration von Partikeln im dünnen Film befindet. Leider konnten die Daten nicht hinsichtlich der Lage der Partikel analysiert werden. Zum ersten Mal wurden dünne flüssige Filme mit Kleinwinkelröntgenstreuung unter streifendem Einfall (GISAXS) analysiert. Mit Hilfe dieser Methode sollte eine laterale Anordnung der Partikel im Film untersucht werden. Es konnten erfolgreiche Messungen durchgeführt werden und mit Hilfe der rechnergestützten Analyse konnte eine Aussage gemacht werden, dass ~ 6 nm große Teilchen in ~ 43 nm Abstand sich im Film befinden. Die Aussage bezüglich der kleinen Teilchen könnte sich auf einzelne, kleinere Partikel beziehen, allerdings könnten auch die 43 nm eine relevante Strukturgröße darstellen, da es in der Dispersion gehäuft Aggregate mit dem Durchmesser in dem Größenbereich vorliegen. Zusammenfassend können sich mit dieser Arbeit die dünnen flüssigen Filme als eine wichtige Kernmethode der Untersuchung von nanofluidischen Prozessen, wie sie häufig in der Natur vorkommen, behaupten.
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Almeida, Maria de Lurdes Soares de. "Selective protection of polyamines : Synthesis of spermidine derivatives." Doctoral thesis, Universidade do Porto. Reitoria, 1989. http://hdl.handle.net/10216/10192.

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Almeida, Maria de Lurdes Soares de. "Selective protection of polyamines : Synthesis of spermidine derivatives." Tese, Universidade do Porto. Reitoria, 1989. http://hdl.handle.net/10216/10192.

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Nadaraia-Hoke, Shorena Flanagan John M. "Structural and thermodynamic characterization of spermidine and spermine synthases." [University Park, Pa.] : Pennsylvania State University, 2009. http://etda.libraries.psu.edu/theses/approved/PSUonlyIndex/ETD-4555/index.html.

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Choucair, Bassima. "Synthèse des polyaminostérols, analogues de la squalamine, à propriétés antibiotique et anticancéreuse." Caen, 2003. http://www.theses.fr/2003CAEN3098.

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La squalamine est un polyaminostérol 3 -N-[3N-(4-aminobutyl)aminopropyl] aminocholestan-7 -ol-24R-sulfate qui a été isolé en 1993 de tissus du requin " Squalus acantias ". Cette molécule a montrée des propriétés antibiotique et anticancéreuse par inhibition de l'angiogénèse dans les tumeurs solides. Cependant, la squalamine est présente en quantités infimes dans les tissus du requin et sa synthèse nécessite plusieurs étapes. D'où l'intérêt de synthétiser des analogues désulfatés de la squalamine. Le polyamine a été introduit en position C-7 du cholestérol, en positions C-7 et C-6 du cholestanol. Ces analogues sont les premiers exemples où la position de la spermidine a été modifiée. Les analogues synthétisés de la squalamine montrent un large spectre d'activité antimicrobienne. Ils présentent également une cytotoxicité vis-à-vis des cellules cancéreuses NSCLC-N6 (CI 50 < 3. 3 g/ml). La squalamine devient le chef de file d'une nouvelle famille d'antibiotiques et d'anticancéreux.
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Catros, Véronique. "Implication des polyamines dans les processus proliferatifs malins." Rennes 1, 1990. http://www.theses.fr/1990REN1T085.

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Des etudes effectuees in vivo chez l'animal ainsi que chez des patients atteints de differents types histologiques de tumeurs, ont permis de montrer que le taux erythrocytaire de spermidine (spd) se comportait comme un index sanguin de la proliferation cellulaire tumorale. L'etude in vivo du metabolisme des polyamines (pa) apres administration de precurseurs radiomarques a des animaux porteurs de tumeurs greffees a permis d'apporter la preuve de l'origine tumorale de la spermidine erythrocytaire. Les modalites d'uptake des pa par les erythrocytes ont par ailleurs ete caracterisees in vitro: les erythrocytes de souris cancereuses presentent des modifications de leurs proteines stromales, leur conferant la capacite de capter trois fois plus de #1#4c spd que les erythrocytes de souris saines. A l'inverse de ce qui se passe au cours d'un processus proliferatif controle, ou l'evolution des taux erythrocytaires de pa est parfaitement regulee, en cas de cancer, les pa s'accumulent dans les hematies. Cette accumulation est correlee a une reduction de la concentration tissulaire en malonaldehyde (mda), une molecule inhibitrice de la replication de l'adn. L'etude du catabolisme des pa dans les cellules cancereuses ainsi que l'utilisation de drogues modulatrices de ce metabolisme permettent d'evoquer l'hypothese d'un role des pa erythrocytaires dans le controle homeostatique de la proliferation cellulaire. Les effets biologiques obtenus avec des analogues tetramethyles de pa permettent egalement d'envisager l'utilisation du metabolisme des pa a des fins therapeutiques et diagnostiques en cancerologie
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Sanvura, Bashwira. "Myricoïdine et dihydromyricoïdine, nouveaux alcaloïdes macrocycliques dérivés de la spermidine." Doctoral thesis, Universite Libre de Bruxelles, 1990. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/213135.

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Suttmann, Rebecca T. "Purification and inhibition of spermidine N⁸-acetyltransferase from rat liver nuclei." Scholarly Commons, 1991. https://scholarlycommons.pacific.edu/uop_etds/2222.

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The naturally occurring polyamines play an essential role in cell growth and proliferation. The enzyme spermidine N8- acetyltransferase catalyzes the acetylation of spermidine utilizing acetyl-CoA as the acetyl donor. In this study, an in vitro acetyltransferase assay was used to determine the types of compounds which can inhibit this reaction. The enzyme was partially purified from rat liver nuclei and solubilized in 0.4 M KCl. The Km for spermidine was 0.47 mM. Studies on the nature of the active site indicated that: (i) a sulfhydryl group is essential for optimal activity as shown by inhibition with parahydroxymercuribenzoate and N -ethylmaleimide, (ii) a metal ion does not appear to be necessary for catalytic activity of this enzyme since EDTA, 2,2-dipryridil, and 1,10 phenanthroline were poor inhibitors of this enzyme, (iii) a lysine or another primary amine is likely to play a crucial role in this reaction since succinate anhydride and 2,4,6-trinitrobenzene sulfonic acid were effective inhibitors of this reaction and (iv) tyrosine is not likely present at the catalytic site since N -acetylimidazole produced no inhibition.
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9

Caron, Édith. "Étude de l'interaction entre l'actine et les polyamines : spermidine et spermine." Thèse, Université du Québec à Trois-Rivières, 1987. http://depot-e.uqtr.ca/5803/1/000560724.pdf.

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Garcie, Christophe. "Modulation atypique de la biosynthèse de la colibactine, une génotoxine de Escherichia coli, ou comment un îlot génomique est en symbiose avec le chromosome bactérien." Thesis, Toulouse 3, 2016. http://www.theses.fr/2016TOU30283/document.

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L'îlot génomique pks code une machinerie de biosynthèse complexe synthétisant la colibactine, une génotoxine produite par certaines souches de Escherichia coli. Cette génotoxine induit des cassures double-brin de l'ADN sur les cellules eucaryotes in vitro et in vivo. La colibactine n'est pas une protéine, mais un métabolite secondaire de type polycétide/peptide non-ribosomal (PK/NRP). Des résultats préliminaires de l'équipe semblaient indiquer que certains gènes du core genome de E. coli seraient également impliqués dans la production de la colibactine. L'objectif de cette thèse était d'identifier les gènes non-essentiels de E. coli situés hors de l'îlot génomique pks impliqués dans la synthèse de colibactine, en construisant une banque de mutants par insertion de transposons. Ce criblage a permis d'identifier 29 gènes candidats, mais deux groupes de gènes ont été particulièrement étudiés dans la suite du projet : trois gènes codants des protéines chaperons, et trois gènes codant des enzymes impliquées dans le métabolisme des polyamines. Le premier projet a permis de montrer que la protéine chaperon HtpG (ou Hsp90Ec), homologue bactérien de la protéine de choc thermique eucaryote Hsp90, est requise pour la production de colibactine, mais aussi de yersiniabactine, un sidérophore (ou système bactérien de captation du fer) appartenant à la même famille chimique que la colibactine. De plus, la protéase ClpQ intervient de concert avec Hsp90Ec dans la production de colibactine et de yersiniabactine. Ces résultats confirment ainsi l'interconnexion entre la synthèse des deux facteurs de virulence de E. coli, la colibactine et la yersiniabactine. Enfin, l'analyse des effets de la mutation du gène htpG au cours d'une infection systémique chez l'animal, dans des modèles de sepsis et de méningite néonatale chez les rongeurs, démontre le rôle de la protéine de réponse au stress Hsp90Ec dans la virulence de E. coli. Le second projet a révélé que les polyamines sont impliquées dans la production de colibactine. L'étude du métabolisme des polyamines par une approche de microbiologie moléculaire a démontré que la spermidine est la polyamine nécessaire à la production de colibactine. Les résultats préliminaires de ce projet indiquent que la spermidine participerait à la régulation de l'expression de certains gènes de l'îlot génomique pks, et de fait modulerait la biosynthèse de colibactine. Des études complémentaires sont en cours pour élucider les mécanismes impliqués. Les résultats de cette thèse sont une illustration parfaite de l'intégration symbiotique d'un élément génétique mobile acquis au cours de l'évolution au sein du chromosome bactérien, grâce à plusieurs connexions bilatérales permettant la production de facteurs de virulence par E. coli
The pks genomic island codes a complex biosynthetic assembly line that synthetizes the colibactin, a genotoxin produced by some strains of Escherichia coli. This genotoxin generates DNA double-strand breaks in eukaryotic cells both in vitro and in vivo. Colibactin is not a protein, but a secondary metabolite belonging to the chemical family of hybrid polyketide/nonribosomal peptide compounds. Preliminary results from our research team suggested that certain genes of the E. coli core genome (i.e. genes present in all strains of the species) could also be involved in the colibactin production. The main goal of this thesis was to identify non-essential E. coli genes located outside the pks island that are required for colibactin biosynthesis, with the screening of a transposon mutant library. This revealed 29 potential candidate genes, but the project focused specifically on two groups of genes: three genes encoding chaperone proteins, and three genes encoding enzymes involved in polyamines metabolism. The first project highlighted the role of the molecular chaperone HtpG (or Hsp90Ec), the bacterial homolog of eukaryotic heat shock protein 90, in the production of colibactin, but also yersiniabactin, a siderophore (i.e. a bacterial iron uptake system) that belongs to the same chemical family as colibactin. Furthermore, the ClpQ protease was involved in colibactin and yersiniabactin production in combination with Hsp90Ec. These results confirmed the interplay between the biosynthesis of two E. coli virulence factors, colibactin and yersiniabactin. Finally, analysis of the effects of htpG disruption during systemic infection in animals, using rodent models of sepsis and neonatal meningitis, demonstrated the role of the stress-responsive molecular chaperone Hsp90Ec in E. coli virulence. The second project revealed the involvement of polyamines in the biosynthesis of colibactin. A molecular microbiology approach demonstrated that spermidine was the polyamine required for colibactin production. Preliminary results suggested that spermidine could regulate the expression of some pks island genes, and therefore could modulate colibactin production. Further experiments are in progress to elucidate the molecular mechanisms involved in this regulation. Together, the results of this thesis perfectly illustrate the symbiotic integration of a mobile genetic element acquired during evolution into the bacterial chromosome, through several crosstalks allowing the production of virulence factors in E. coli
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Books on the topic "Spermidina"

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Sobolev, Victor S. The peanut plant and light: Spermidines from peanut flowers and studies of their photoisomerization. New York: Nova Science Publishers, 2010.

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Sobolev, Victor S. The peanut plant and light: Spermidines from peanut flowers. Hauppauge, N.Y: Nova Science Publishers, 2010.

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Klose, Markus. Effects of a hydroxy-cinnamoyl conjugate of spermidine in the neuromuscular junction of crayfish. St. Catharines, Ont: Brock University, Dept. of Biological Sciences, 2000.

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Tower, Paula Allene. Homospermidine, spermidine, and putrescine: The biosynthesis and metabolism of polyamines in Rhizobium meliloti. 1987.

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Book chapters on the topic "Spermidina"

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Schomburg, D., M. Salzmann, and D. Stephan. "Spermidine dehydrogenase." In Enzyme Handbook 7, 195–97. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-78521-4_40.

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Schomburg, Dietmar, and Dörte Stephan. "Spermidine synthase." In Enzyme Handbook 13, 63–68. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-59176-1_13.

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Rips, R., and C. Guette. "Determination of Polyamines (Spermine, Spermidine, Putrescine) in Biological Samples." In Contemporary Electroanalytical Chemistry, 299–302. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4899-3704-9_33.

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Zahedi, Kamyar, and Manoocher Soleimani. "Spermidine/Spermine-N1-Acetyltransferase in Kidney Ischemia Reperfusion Injury." In Methods in Molecular Biology, 379–94. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-034-8_24.

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Nampoothiri, Madhavan, Kiran Kumar Kolathur, Runali Sankhe, and Sairaj Satarker. "Spermidine, an Autophagy Inducer, as a Therapeutic Antiaging Strategy." In Emerging Anti-Aging Strategies, 135–53. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-7443-4_8.

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Feirer, R. P., S. R. Wann, and D. W. Einspahr. "The effects of spermidine synthesis inhibitors on in-vitro plant development." In Polyamines in Plants, 117–25. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5171-6_10.

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Perin, Antonio, Angela Sessa, and M. Alfonsina Desiderio. "Estrogenic Control of Spermidine/Spermine N1-Acetyltransferase Activity in Rat Uterus." In Progress in Polyamine Research, 345–51. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5637-0_30.

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Sunkara, Prasad S., John H. Zwolshen, Nellikunja J. Prakash, and Terry L. Bowlin. "Mechanism of Antitumor Activity of Norspermidine, a Structural Homologue of Spermidine." In Progress in Polyamine Research, 707–16. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5637-0_62.

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Tortolini, Cristina, Gabriele Favero, and Franco Mazzei. "Development of Amine-Oxidase-Based Biosensors for Spermine and Spermidine Analysis." In Methods in Molecular Biology, 75–80. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7398-9_7.

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Tabor, Herbert, and Celia White Tabor. "Biosynthesis and Metabolism of 1,4-diaminobutane, Spermidine, Spermine, and Related Amines." In Advances in Enzymology - and Related Areas of Molecular Biology, 203–68. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470122815.ch7.

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Conference papers on the topic "Spermidina"

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KOPEL, Pavel, Natalia CERNEI, Pavel HORKY, Zuzana LACKOVA, Vedran MILOSAVLJEVIC, Milica GAGIC, Ondrej ZITKA, and Vojtech ADAM. "MAGHEMITE PARTICLES FOR SPERMIDINE SEPARATION." In NANOCON 2019. TANGER Ltd., 2020. http://dx.doi.org/10.37904/nanocon.2019.8496.

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KATSUMATA, SHUNJI, HARUNORI KIGASAWA, and KUNIHARU UTSUNO. "AFM STUDY ON CONDENSATION OF DNA BY SPERMIDINE." In Proceedings of the 8th Asia-Pacific Physics Conference. WORLD SCIENTIFIC, 2001. http://dx.doi.org/10.1142/9789812811523_0146.

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Shkodra, Bajramshahe, Mattia Petrelli, Martina Costa Angeli, AKM Sarwar Inam, Enrico Avancini, Niko Munzenrieder, Paolo Lugli, and Luisa Petti. "Flexible carbon nanotube-based electrolyte-gated field-effect transistor for spermidine detection." In 2021 IEEE International Conference on Flexible and Printable Sensors and Systems (FLEPS). IEEE, 2021. http://dx.doi.org/10.1109/fleps51544.2021.9469788.

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Murayama, Yoshihiro. "Reentrant Collapsing Transition of Single DNA Molecules: Elastic Response Depending on Spermidine Concentration." In SLOW DYNAMICS IN COMPLEX SYSTEMS: 3rd International Symposium on Slow Dynamics in Complex Systems. AIP, 2004. http://dx.doi.org/10.1063/1.1764157.

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Brett-Morris, Adina, Scott M. Welford, Eli Bar, Raffaella Spina, Bradley Wright, Junran Zhang, Jun Lu, and Yuji Seo. "Abstract 3954: SAT1 (Spermidine/spermine-N1-acetyltrasferase 1) promotes radiation resistance in glioblastoma multiforme." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-3954.

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Shaker, Husham A., and Jwan A. Zainulabdeen. "Estimation the levels of spermidine and spermine in sera of inflammatory bowel disease patients." In PROCEEDING OF THE 1ST INTERNATIONAL CONFERENCE ON ADVANCED RESEARCH IN PURE AND APPLIED SCIENCE (ICARPAS2021): Third Annual Conference of Al-Muthanna University/College of Science. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0093817.

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Keller, Nicholas, and Douglas E. Smith. "Single-molecule studies of the effect of spermidine on DNA mechanics and viral DNA packaging." In SPIE NanoScience + Engineering, edited by Kishan Dholakia and Gabriel C. Spalding. SPIE, 2012. http://dx.doi.org/10.1117/12.930381.

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Park, S. W., M. Kim, A. R. Baek, J. H. Lee, A. S. Jang, and D. Kim. "Spermidine Attenuated Bleomycin Induced Lung Injury/Fibrosis by Inhibition of Endoplasmic Reticulum Stress (ers) Induced Apoptosis and Cellular Senescence." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a5228.

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Lu, Jun, Mingming Li, Hong Xu, Malcolm Tingle, and Garth J. S. Cooper. "Abstract LB-287: Spermidine/spermine actyltransferase is responsible for the metabolism of triethylenetetramine and other polyamine analogs in humans." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-lb-287.

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Kuo, Be-Sheng, Gil Korner, and Thorir D. Bjornsson. "ROLE OF POLYAMINES IN THE REGULATION OF SYNTHESIS AND SECRETION OF PLASMINOGEN ACTIVATOR FROM BOVINE AORTIC ENDOTHELIAL CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644655.

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The effects of three polyamines, putrescine (PUT), spermidine (SPD) and spermine (SPM), were investigated on the synthesis and secretion of plasminogen activator (PA) and antiactivator (PAI) activities in confluent bovine aortic endothelial cells. PA activity was determined bythe fibrin plate method, and individual species with PA and PAI activities were separated and visualized using SDS-PAGE with zymography and reverse fibrin autography. Both control cells and cells treated with polyamines secreted PA activity in a time-dependent fashion. After 24-hour incubation, the three polyamines enhanced PA secretion in a dose-dependent manner (10-6 to 2.5 × 10-3 M), with a potency order of SPM > SPD> PUT, as estimated by the fibrin plate method. The maximum PA releases after PUT (0.5 mM), SPD (2.5 mM) and SPM (0.5 mM) were 1.7, 4.5 and 5.4 times control levels, respectively. Concentrations lower than 1 μM had essentially no effects. The enhancement of PA activity by polyamines was blocked by actino-mycin D and cycloheximide, while it was not affected by inhibitors of polyamine biosynthesis except that the enhancement by PUT (0.5 mM) was reduced by methylglyoxal bis(guanylhydrazone). These data suggest that polyamines directly stimulate PA synthesis and secretion through promotion of gene transcription and translation, and that this effect appears to be related to their position in the biosynthetic pathway of polyamines. The kinetic patterns of activities of ornithine decarboxylase and S-adenosy-methionine decarboxylase in confluent endothelial cells stimulated withfresh culture medium suggest that there is rapid turnover of intracellular polyamines. Multiple forms of secreted PA were observed and both tissue- and urokinase-type PA were enhanced by polyamines, while the PAI activity, as evaluted by reverse fibrin autograpy, was apparently reduced. These experimental results suggest that polyamines may play an important role in the regulation of the synthesis and secretion of plasminogen activators, and that this biological function could be modified by disease states and by agents that are associated with altered polyamine metabolism.
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