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Xie, Lan, Rui Ma, Chao Han, Kai Su, Qiufang Zhang, Tian Qiu, Lei Wang, et al. "Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device." Clinical Chemistry 56, no. 8 (August 1, 2010): 1270–78. http://dx.doi.org/10.1373/clinchem.2010.146902.
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BACKGROUND Sperm screening is an essential step in in vitro fertilization (IVF) procedures. The swim-up method, an assay for sperm motility, is used clinically to select the ideal sperm for subsequent manipulation. However, additional parameters, including acrosome reaction capability, chemotaxis, and thermotaxis, are also important indicators of mammalian sperm health. To monitor both sperm motility and chemotaxis simultaneously during sperm screening, we designed and constructed a microdevice comprising a straight channel connected with a bibranch channel that mimics the mammalian female reproductive tract. METHODS The width and length of the straight channel were optimized to select the motile sperms. We selectively cultured cumulus cells in the bibranch channel to generate a chemoattractant-forming chemical gradient. Sperm chemotaxis was represented by the ratio of the sperm swimming toward different branches. RESULTS The percentage of motile sperms improved from 58.5% (3.8%) to 82.6% (2.9%) by a straight channel 7 mm in length and 1 mm in width. About 10% of sperms were found to be chemotactically responsive in our experiment, which is consistent with previous studies. CONCLUSIONS For the first time, we achieved the combined evaluation of both sperm motility and chemotaxis. The motile and chemotactically responsive sperms can easily be enriched on a lab-on-a-chip device to improve IVF outcome.
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S. Vishwekar, Pallavi, Nikita Lad, Mamta Shivtare, and Pradnya Shetty. "ICSI outcome in surgically retrieved sperm compared with ejaculated sperm control." International Journal of Reproduction, Contraception, Obstetrics and Gynecology 8, no. 3 (February 26, 2019): 869. http://dx.doi.org/10.18203/2320-1770.ijrcog20190847.
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Background: Globally, the prevalence of infertility is around 10% of the total population. 30% of these have male factor infertility. Azoospermia is found in 1% of men, in 20% of which, the etiology is a bilateral obstruction of the male genital tract while others have non obstructive azoospermia. In azoospermic men sperms are microsurgically retrieved from epididymis and testes by TESA and PESA respectively. The aim of this study was to evaluate the outcomes of intracytoplasmic sperm injection ICSI using surgically retrieved sperm of azoospermic men either obstructive or nonobstructive and to compare it with ejaculated sperms in men having severe oligospermia.Methods: This was retrospective cohort study conducted based on the data collected from our reproductive endocrinology and infertility unit, 126 ICSI cycles performed during the period of 5 years were taken and divided into two groups, one with patients having ejaculated sperms with oligospermia and other group with patients who had surgically retrieved normal sperms due to azoospermia. Outcome of these ICSI cycles included fertilization, cleavage, biochemical and clinical pregnancy was assessed.Results: In present study it was found that ICSI outcome was comparable in both the groups with ejaculated sperm and surgically retrieved sperm as fertilization rate (72% vs 65%), Implantation Rate (58 vs 51%), clinical pregnancy rate (CPR) (51% vs 44.82%) observed with ejaculated or retrieved sperm group respectively showed no statistical difference.Conclusions: Present study shows that minimally invasive techniques of PESA and TESA can be successfully performed to retrieve sperm for ICSI in the treatment of azoospermic men which gives them the chance to father their biological child. The result of this study indicates that treatment outcomes of PESA/TESA-ICSI cycles compare favourably with that of ICSI using ejaculated sperm.
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Kaliky, Nunun Ainun Putri Sari Banun, Mia Setiawati, Odang Carman, and Nur Bambang Priyo Utomo. "Effect of zinc (Zn) supplementation on quality and quantity of striped catfish Pangasianodon hypophthalmus sperm." Jurnal Akuakultur Indonesia 18, no. 1 (January 18, 2019): 46–53. http://dx.doi.org/10.19027/jai.18.1.46-53.
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ABSTRACT This study aimed to evaluate the effects of Zinc (Zn) supplementation on the quality and quantity of striped catfish sperm. Experimental design for this study was a complete randomized design with five treatments and five replications. Male broods fed with Zn supplementation for eight weeks. The Zn supplemented into the fish diet at different concentrations (0, 50, 100, 150 and 200 mg/kg of feed). The results showed that Zn supplementation could improve the quality and quantity of striped catfish sperm. The treatments also showed significant effects on semen volume, sperm motility, sperm viability, and sperm concentration (P<0.05). Zn supplementation at a dose of 200 mg/kg feed demonstrated the best result has indicated by enhancement of quality and quantity of striped catfish sperm, increasing 51% of the volume, 11.6% of motility, 5.81% of viability, 54.1% of concentrations. The results suggested that Zn played an important role in improving reproductive performances of male striped catfish reproduction. Keywords: quality of sperm, a quantity of sperm, striped catfish, supplementation zinc ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi Zinc (Zn) terhadap kualitas dan kuantitas sperma ikan patinPangasianodon hypophthalmus. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan danlima ulangan. Induk jantan diberi pakan dengan suplementasi Zn selama 8 minggu. Zn disuplementasikan dengan dosis berbeda (0, 50, 100, 150 dan 200 mg/kg pakan). Hasil penelitian menunjukkan bahwa suplementasi Zn dapat meningkatkan kualitas dan kuantitas sperma ikan patin sehingga berpengaruh signifikan terhadap volume semen, motilitas, viabilitas dan konsentrasi sperma (P<0,05). Suplementasi Zn pada dosis pakan 200 mg/kg menunjukkan hasil terbaik yang ditunjukkan oleh peningkatan kualitas dan kuantitas sperma ikan patin 51% volume; 11,6% motilitas; 5,81% viabilitas; 54,1% konsentrasi sperma. Hasil penelitian ini menunjukkan bahwa Zn memainkan peran penting dalam meningkatkan reproduksi ikan patin. Kata kunci: kualitas dan kuantitas sperma, Ikan patin, suplementasi Zn
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Salama, Nader, and Omer Sirelkhatim Hassan. "Staged Laboratory Processing of Testicular Tissue in Non-Obstructive Azoospermia May Rescue Retrieving an Existing Sperm: A Case Report and Literature Review." Clinical Medicine Insights: Case Reports 16 (January 2023): 117954762311783. http://dx.doi.org/10.1177/11795476231178353.
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Non-obstructive azoospermia (NOA) is the most difficult form of male infertility to manage. It usually requires sperm retrieval from the testis, which is most challenging due to sperm rarity. Here, we describe the recovery of testicular sperms that had been missing and whose original retrieval results were negative. Salvage microsurgical testicular sperm extraction and sperm testing were performed on a 36-year-old male with NOA. Neither in the operation room nor after an inspection in the embryology laboratory were any sperm detected. The obtained tissue was advised to be frozen because the patient data and surgical microscopy predicted a favorable outcome, and the tissue processing was done in an inappropriate environment. About 1 month later, the specimen was thawed, crushed, and re-examined. Successful oocyte fertilization resulted from an effective detection of sperms and their direction to intra-cytoplasmic sperm injection. This is the first case report that, to the best of our knowledge, describes the stepwise laboratory processing of testicular tissue and its capacity to recover lost sperms in challenging NOA cases and under less-than-ideal working conditions.
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Sudipta Chowdhury and Samarendra Nath Banerjee. "Genotoxic activity of betel nut on germinal cell in Sarcoma 180 ascites tumour bearing male mice." GSC Biological and Pharmaceutical Sciences 16, no. 2 (August 30, 2021): 171–81. http://dx.doi.org/10.30574/gscbps.2021.16.2.0235.
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The genotoxicity of the ethanolic extract of betel nut was evaluated using sarcoma 180 tumour bearing mouse considering sperm motility, sperm viability, biochemical estimation of fructose in seminal fluid and sperm head morphology assays. Sperm head morphology was studied by H-E staining and Toluidine blue staining method. But Toluidine blue staining method is a reliable method to evaluate the DNA damage of sperms. Ethanolic BNE (betel nut extract) can suppress the percentage of sperm motility, sperm viability and seminal fructose level. In addition, it can also enhance the percentage of DNA damaged sperms. Moreover, histological sections of testes have been studied in control and BNE treated sarcoma 180 tumour bearing mice to highlight the potential toxic effect of BNE. The significant decreasing rate of seminal fructose concentration, sperm motility as well as viability and increasing rate of sperm head abnormality in different doses of treated series may be as a result of different toxic alkaloid ingredients present in BNE. Therefore, the results showed the potential of the BNE to induce different types of germ cell abnormalities in tumour bearing male mice.
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Akbar, Fahmi, Agus Oman Sudrajat, and Siti Subaidah. "Sperm quality of Litopenaeus vannamei broostock injected by PMSG and antidopamin." Jurnal Akuakultur Indonesia 14, no. 2 (October 15, 2015): 98. http://dx.doi.org/10.19027/jai.14.98-103.
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<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">The important role in determining the productivity of shrimp was the quality and quantity of shrimp sperm. The decreasing of hatching rate was predicted as the effect of the decreasing quality of sperm. It then could influence the number and quality of naupli produced. Hormonal induction of maturation is one of alternative solution that can improve shrimp sperm quality. This study was conducted to examine the effect of pregnant mare serum gonadotropin (PMSG) and antidopamine (AD) injection on white shrimp <em>Litopenaeus vannamei</em> sperm quality. This research consisted of six treatments which were treatment without eyestalk ablation, eyestalk ablation, and premix PMSG hormone, and AD at the dose of 0.1 mL/kg, 0.25 mL/kg, 0.5 mL/kg, and 1 mL/kg. The observed parameters were sperm count and percentage of normal and abnormal sperm. The results showed that PMSG hormone and AD injection could improve sperm quality of <em>L. vannamei</em> shrimp. Hormone at the dose of 0.25 mL/kg and 0.5 mL/kg were the optimal doses to increase sperm count and the percentage of normal sperm, also to lower the percentage of abnormal sperm.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keyword: PMSG, AD, sperm quality, <em>Litopenaeus vannamei</em></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kuantitas dan kualitas sperma udang jantan sangat berperan penting dalam menentukan produktivitas udang. Terjadinya penurunan daya tetas telur udang diduga karena terjadinya penurunan kualitas sperma. Hal ini berpengaruh terhadap jumlah dan kualitas nauplius yang diproduksi. Induksi maturasi secara hormonal merupakan salah satu alternatif yang dapat meningkatkan kualitas sperma udang. Penelitian ini dilakukan untuk mengkaji pengaruh penyuntikan <em>pregnant mare serum gonadotropin</em> (PMSG) dan antidopamin (AD) terhadap kualitas sperma udang vaname <em>Litopenaeus vannamei</em>. Penelitian terdiri atas enam perlakuan, yaitu perlakuan tanpa ablasi mata, ablasi mata, dan injeksi dengan premix hormon PMSG dan AD dosis 0,1 mL/kg, 0,25 mL/kg, 0,5 mL/kg, dan 1 mL/kg. Parameter yang diamati jumlah sperma, persentase sperma normal dan abnormal. Hasil penelitian menunjukkan penyuntikan hormon PMSG dan AD dapat meningkatkan kualitas sperma udang <em>L. vannamei</em>. Hormon dosis 0,25 mL/kg dan 0,5 mL/kg merupakan dosis optimal dalam meningkatkan jumlah sperma dan persentase sperma normal, serta mengurangi persentase sperma abnormal.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: PMSG, AD, kualitas sperma, <em>Litopenaeus vannamei</em></p><p> </p>
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Dewi, Raden Roro Sri Pudji Sinarni, Alimuddin Alimuddin, Agus Oman Sudrajat, Komar Sumantadinata, and Sularto Sularto. "OPTIMAL ELECTROPORATION CONDITION FOR SPERM MEDIATED GENE TRANSFER IN STRIPPED CATFISH (Pangasionodon hypophthalmus)." Indonesian Aquaculture Journal 5, no. 1 (June 30, 2010): 1. http://dx.doi.org/10.15578/iaj.5.1.2010.1-10.
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The success of transgenic fish production has been achieved through eggs fertilization using electroporated sperms carrying exogenous DNA. This study was conducted in order to obtain the optimal electroporation condition for stripped catfish sperm. A plasmid containing green fluorescent protein (GFP) gene driven by carp β-actin promoter was transferred into sperm using electrophoresis method towards transgenic stripped catfish (Pangasionodon hypophthalmus) production. Electroporation was carried out using square wave shock with pulse length of 30 ms and pulse interval of 0.1 sec. Treatments are combination between voltage (50 V, 75 V, and 100 V) and pulse number (1 and 3). Exogenous DNA concentration used was 10 μg/mL of Tris-EDTA. Results showed that increasing the voltage from 50 to 100 decreased sperm motility, while pulse number did not affect sperm motility. Voltage of 50 gave the best motility of sperm, although sperm viability relatively similar between treatments and control except at 100 V with 3 pulses number. Further, electroporation-treated sperms were able to fertilize eggs. Higher hatching rate of eggs was obtained in electroporation treatment at 50 V with pulse number of 1 and 3. The persistence of transferred GFP was detected in electroporated and incubated sperms (control). However, GFP was only detected in larvae from eggs that were fertilized by electroporated sperm. Thus, electroporation could be applied to produce transgenic stripped catfish.
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Rahayu, Vitaloka Guci, and Evi Hanizar. "The Effect of Lemon (Citrus limon) Extracts On The Quantity and Quality of Mice (Mus musculus) Sperm." Elkawnie 7, no. 2 (January 17, 2022): 300. http://dx.doi.org/10.22373/ekw.v7i2.9389.
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Abstract: Vitamin C has been proved as a nutrient to improve the quality of sperm. Society believed that consuming the lemons could potentially enhance the sperm quality of humans. However, the appropriate concentration should be well studied to obtain the optimum concentration to improve the sperm quality and quantity. The present research tried to provide information on how lemon could improve the sperm quality by designing a true experimental using a series concentration of lemon extract (25%, 50% and 75 % concentrations) given to the male mice (Mus musculus). The investigation was made by giving the lemon treatment three times a day for 5 weeks. To investigate the effect of lemon extract, the mice sperm were taken from the epididymis and observed using a multimedia microscope and counted using Neubauer’s counting rooms, while motility and morphology were observed using object-glass. The result showed that the high concentration of lemon could not provide the greatest improvement of sperm quality and quantity. The optimum condition was seen in 25% of lemon extract, where the increase of lemon concentration suppressed the lemon improvement effect, which reduced the sperm quality and quantity. However, the improvement was still made if the result was compared to control, meaning consuming lemon was better than consuming any lemon treatment. The result was in accordance with quality improvement of sperm, where 25% of lemon concentration treatment provided the highest average motility and normal morphology of sperm. However, the high concentration of lemon extract (50% and 75% of lemon concentrations) provided a lower effect due to the adverse effect. The result proved that lemon could be used to boost the quality and quantity of sperm in an appropriate concentration where the excess lemon extract could reduce the effect of lemon in improving sperm quality and quantity.Abstrak: Vitamin C telah terbukti sebagai nutrisi untuk meningkatkan kualitas sperma dimana masyarakat percaya bahwa mengkonsumsi lemon berpotensi meningkatkan kualitas sperma manusia. Namun, konsentrasi yang tepat harus dipelajari dengan baik untuk mendapatkan konsentrasi yang optimal untuk meningkatkan kualitas dan kuantitas sperma. Penelitian ini mencoba memberikan informasi bagaimana lemon dapat meningkatkan kualitas sperma dengan merancang eksperimen nyata menggunakan serangkaian konsentrasi ekstrak lemon (konsentrasi 25%, 50% dan 75%) yang diberikan kepada mencit jantan (Mus musculus). Penyelidikan dilakukan dengan memberikan ekstrak lemon sebanyak tiga kali dalam sehari selama 5 minggu. Untuk mengetahui pengaruh ekstrak lemon, sperma mencit diambil dari epididimis dan diamati menggunakan mikroskop multimedia dan dihitung menggunakan kamar hitung Neubauer, sedangkan motilitas dan morfologi diamati menggunakan kaca objek. Hasil penelitian menunjukkan bahwa konsentrasi lemon yang tinggi tidak dapat memberikan peningkatan kualitas dan kuantitas sperma yang tertinggi. Kondisi optimum terlihat pada ekstrak lemon 25% dimana peningkatan konsentrasi lemon menekan efek perbaikan lemon yang menurunkan kualitas dan kuantitas sperma. Namun perbaikan tetap dilakukan jika hasilnya dibandingkan dengan kontrol, artinya mengkonsumsi lemon lebih baik daripada tanpa mengkonsumsi lemon. Hasil tersebut sesuai dengan peningkatan kualitas sperma dimana perlakuan konsentrasi lemon 25% memberikan rata-rata motilitas dan morfologi sperma yang normal paling tinggi. Namun, konsentrasi tinggi ekstrak lemon (50% dan 75% konsentrasi lemon) memberikan efek yang lebih rendah karena efek samping. Hasil penelitian membuktikan bahwa lemon dapat digunakan untuk meningkatkan kualitas dan kuantitas sperma dalam konsentrasi yang sesuai dimana kelebihan ekstrak lemon dapat mengurangi efek lemon dalam meningkatkan kualitas dan kuantitas sperma.
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SWAIN, DILIP KUMAR, PRASANT SWARNKAR, JITENDER KUMAR, and SARVAJEET YADAV. "Evaluation of in vitro longevity of caprine cauda epididymal sperms at different storage intervals of time." Indian Journal of Animal Sciences 82, no. 11 (November 20, 2012): 1347–50. http://dx.doi.org/10.56093/ijans.v82i11.25144.
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The present study was designed to evaluate the effect of both temperature and storage time interval on the cauda epididymal sperm quality in bucks. The testes collected in normal saline at 4°C were exhibited improved sperm attributes at all periods of storage temperature as compared to the testes collected in normal saline at room temperature as well as preserved at the same temperature. In both cases, with the passage of time of storage the sperm features started deteriorating. That is why the study recommended that to maintain the sperm attributes for a longer period of storage, suitable antioxidants and amino acids may be supplemented. The sperms should be isolated as soon as possible after the collection of the testes to maintain the integrity and quality of the sperm from the cauda. It was evident that, as soon as possible, the sperms from the cauda should be recovered as well as storage temperature should be lesser than 4°C. Suitable strategies should be developed to cryopreserve the cauda epididymal sperms.
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Tian, Fang-Bao, and Li Wang. "Numerical Modeling of Sperm Swimming." Fluids 6, no. 2 (February 7, 2021): 73. http://dx.doi.org/10.3390/fluids6020073.
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Due to rising human infertility, sperm motility has been an important subject. Among the hundreds of millions of sperms on the journey up the oviducts, only a few excellent travelers will reach the eggs. This journey is affected by many factors, some of which include sperm quality, sperm density, fluid rheology and chemotaxis. In addition, the sperm swimming through different body tracks and fluids involves complex sperm flagellar, complex fluid environment, and multi-sperm and sperm-wall interactions. Therefore, this topic has generated substantial research interest. In this paper, we present a review of computational studies on sperm swimming from an engineering perspective with focus on both simplified theoretical methods and fluid–structure interaction methods. Several open issues in this field are highlighted.
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Tabarez, A., W. García, and M. J. Palomo. "58 EFFECT OF MELATONIN IMPLANT ON BLANCA de RASQUERA BUCKS DURING SPRING ON SPERM MORPHOMETRY BEFORE AND AFTER CRYOPRESERVATION." Reproduction, Fertility and Development 27, no. 1 (2015): 122. http://dx.doi.org/10.1071/rdv27n1ab58.
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In order to improve sperm cryopreservation throughout the year and accelerate the process of preservation of this Catalonian goat breed in extinction danger, we proposed to assess the effect of melatonin implant application in Blanca de Rasquera males during spring on sperm head morphometry of fresh and thawed sperm. Therefore 8 bucks of 30 months old approximately were divided into 2 groups. In one of the groups, 2 melatonin implants (Melovine®, CEVA) were inserted into bucks 60 days before starting the collection of semen, and the other group was kept untreated. Briefly, fresh ejaculates from each group of 4 bucks were collected in spring, immediately mixed in equal quantities, and centrifuged twice (600 g for 10 min). Then the pellet was resuspended in a Tris-based medium containing 15% (v/v) of powdered egg yolk supplemented with 5% glycerol. Afterward, sperm samples were refrigerated at 5°C for 4 h before being frozen in LN vapour. Buck sperm head morphometry was analysed by computer-assisted sperm analysis (ISAS®) on fresh and thawed sperm previously stained with Diff Quick®. Data were analysed by GLM multivariate procedure (IBM SPSS, 2011; mean ± s.e., n = 6), showing significant differences among treatments in all the morphometric parameters except head perimeter and rugosity (Table 1). Our results suggest that melatonin application in bucks increases the ellipticity and elongation of fresh and thawed sperm, meanwhile the cryopreservation process reduces both parameters. Likewise melatonin implants increase significantly the head length only on thawed sperms as cryopreservation process increases the head width, area in sperms from implanted males and regularity only in sperms from nonimplanted bucks. These head changes on fresh and thawed sperm morphometry should be deeply investigated in order to know how they could affect sperm cryosurvival and fertility. Table 1.Effect of melatonin implant on Blanca de Rasquera bucks during spring on morphometry of fresh and thawed sperm This research was supported by INIA (RZ2009–00008–00–00), Generalitat de Catalunya (2009SGR0621 and CUR-DIUE), and FSE and Fundación Carolina.
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Junior, M. Zairin, S. Handayani, and I. Supriatna. "Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15%." Jurnal Akuakultur Indonesia 4, no. 2 (July 1, 2007): 145. http://dx.doi.org/10.19027/jai.4.145-151.
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<p>Attempt to produce a lot number of <em>Tor soro</em> is difficult if only obtained by natural spawning since they often get mature in dissimilar time. Stock of sperm kept in enough number for fertilization in anytime is needed to overcome the problem. Cryopreservation of semen is one of sperm cryopreservation methods using liquid nitrogen (-196<sup>o</sup>C) to quickly freeze the sperm. Several cryoprotectants can be utilized in cryopreservation. In this study, quality by means of motility of cryopreserved sperm using dimethylsulfoxide or glycerol as cryoprotectant in the dose of 5, 10 and 15% were analyzed. The result of study show that percentage of sperm motile after cryopreservation using dimethylsulfoxide and glycerol was similar ranged from 76.7 to 83.3%. Therefore, both cryoprotectant used in this study could be employed for cryopreservation of Tor soro sperm.</p> <p>Keywords: cryopreservation, dimethylsulfoxide, glycerol, sperm, <em>Tor soro</em></p> <p><strong> </strong></p> <p><strong>ABSTRAK</strong></p> <p>Upaya produksi ikan batak (<em>Tor soro</em>) dalam jumlah besar relatif sulit jika hanya mengandalkan hasil pemijahan secara alami karena kematangan gonad antara ikan jantan dan betina sering tidak bersamaan. Untuk mengatasinya diperlukan stok sperma yang disimpan dalam jumlah yang cukup banyak untuk pembuahan yang dapat dipakai setiap saat. Kriopreservasi semen merupakan salah satu cara penyimpanan sperma yang menggunakan larutan nitrogen cair (-196°C) untuk membekukan sperma secara cepat. Beberapa jenis protektan dapat digunakan dalam pembekuan sperma. Pada penelitian ini, dilakukan analisis kualitas dalam arti motilitas sperma hasil kriopreservasi menggunakan dimetilsulfoksida dan gliserol sebagai krioprotektan dengan dosis 5, 10 dan 15%. Hasil penelitian menunjukkan bahwa persentase sperma motil setelah dilakukan kriopreservasi adalah sama, berkisar antara 76,7% hingga 83,35%. Dengan demikian, kedua jenis krioprotektan yang digunakan dalam penelitian ini bisa dipakai dalam kriopreservasi sperma ikan batak.</p> <p>Kata kunci: kriopreservasi, dimetilsulfoksida, gliserol, sperma, <em>Tor soro</em></p>
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Hansen, Jan, Sebastian Rassmann, Jan Jikeli, and Dagmar Wachten. "SpermQ–A Simple Analysis Software to Comprehensively Study Flagellar Beating and Sperm Steering." Cells 8, no. 1 (December 26, 2018): 10. http://dx.doi.org/10.3390/cells8010010.
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Motile cilia, also called flagella, are found across a broad range of species; some cilia propel prokaryotes and eukaryotic cells like sperm, while cilia on epithelial surfaces create complex fluid patterns e.g., in the brain or lung. For sperm, the picture has emerged that the flagellum is not only a motor but also a sensor that detects stimuli from the environment, computing the beat pattern according to the sensory input. Thereby, the flagellum navigates sperm through the complex environment in the female genital tract. However, we know very little about how environmental signals change the flagellar beat and, thereby, the swimming behavior of sperm. It has been proposed that distinct signaling domains in the flagellum control the flagellar beat. However, a detailed analysis has been mainly hampered by the fact that current comprehensive analysis approaches rely on complex microscopy and analysis systems. Thus, knowledge on sperm signaling regulating the flagellar beat is based on custom quantification approaches that are limited to only a few aspects of the beat pattern, do not resolve the kinetics of the entire flagellum, rely on manual, qualitative descriptions, and are only a little comparable among each other. Here, we present SpermQ, a ready-to-use and comprehensive analysis software to quantify sperm motility. SpermQ provides a detailed quantification of the flagellar beat based on common time-lapse images acquired by dark-field or epi-fluorescence microscopy, making SpermQ widely applicable. We envision SpermQ becoming a standard tool in flagellar and motile cilia research that allows to readily link studies on individual signaling components in sperm and distinct flagellar beat patterns.
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Kurd, Soleiman, Sara Hosseini, Fardin Fathi, Vahid Jajarmi, and Mohammad Salehi. "Dimethyl sulphoxide and electrolyte-free medium improve exogenous DNA uptake in mouse sperm and subsequently gene expression in the embryo." Zygote 26, no. 5 (October 2018): 403–7. http://dx.doi.org/10.1017/s0967199418000436.
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SummaryOne of the methods to generate transgenic animals is called sperm-mediated gene transfer (SMGT). Mature sperm cells can take up exogenous DNA molecules intrinsically and transfer them into the oocyte during fertilization. This study assessed the effect of dimethyl sulfoxide (DMSO) and electrolyte-free medium (EFM) on DNA uptake (EGFP–N1plasmid) in mouse sperm. Sperms cells cultured in human tubular fluid (HTF) without any treatment were considered as the control group. Sperms cells that were incubated in EFM and HTF with DNA/DMSO at 4°C were classified into EFM and HTF groups. Sperm motility and viability were assessed following treatment. In vitro fertilization (IVF) with sperm in all groups was performed. Fertilization, embryo development and GFP-positive blastocyst rates were analyzed and compared. The result showed that sperm motility and viability in EFM were better than those in the HTF group. The rate of development to reach the blastocyst stage and GFP-positive blastocysts was significantly higher in the EFM group compared with the HTF group (P<0.05). Our data demonstrate that sperm stored in the EFM group can improve the efficiency of SMGT for the generation of GFP-positive blastocysts.
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BANDULARATNE, ENOKA, and ARIFF BONGSO. "Evaluation of Human Sperm Function After Repeated Freezing and Thawing." Journal of Andrology 23, no. 2 (March 4, 2002): 242–49. http://dx.doi.org/10.1002/j.1939-4640.2002.tb02621.x.
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ABSTRACT: Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to sperm after repeated freezing and thawing. The results showed that normozoospermic and oligozoospermic sperm survived after 3 repeated freeze‐thaw cycles. The inclusion of seminal plasma did not seem to protect human sperm during freezing and thawing. There were no significant differences in recovery percentages for motile, vital, and morphologically normal sperm between slow and rapid freezing methods in thaws 1, 2, and 3 of normozoospermic and oligozoospermic unwashed (u), washed (w), and washed + seminal plasma (ws) samples. However, there were significant percentage drops in the recovery of motile and vital sperm between each thaw (ie, first to second thaw, and second to third thaw) using both slow and rapid freezing for u, w, and ws samples (P < .01). There were also no significant differences in percentage recovery of motile, vital, and morphologically normal sperm between u, w, and ws samples during thaws 1 to 3 in the normozoospermic and oligozoospermic groups. Sperm were capable of fertilizing hamster oocytes microinjected with single sperms after 3 freeze‐thaw cycles as evidenced by the formation of 2 distinct pronuclei and 2 polar bodies in 22.2% and 17.2% of normozoospermic and oligozoospermic samples, respectively. The numbers of normal vital motile sperm after 3 serial freeze‐thaw cycles are adequate for bringing about fertilization via intracytoplasmic sperm injection in ART programs. Thus, leftover washed sperm in laboratories that perform in vitro fertilization can be frozen, thawed, and refrozen several times without loss of the sperms' ability to fertilize. This approach has tremendous benefits for men who have difficulty producing sperm and for those with low and declining sperm counts.
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Goncharenko, I. V., and Yu S. Pelykh. "COMPARATIVE ASSESSMENT OF SEXED AND TRADITIONAL SEMEN OF HOLSTEIN BULLS." Animal Breeding and Genetics 51 (March 28, 2018): 231–39. http://dx.doi.org/10.31073/abg.51.31.
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Holstein dairy cattle are characterized by many outstanding qualities such as exterior constitutive type, level of milk yield per lactation and during the period of economic use, well developed udder in size and shape, adapted to machine milking and others. However, there are some negative aspects at the breeding of animals of this breed. This is a short period of practical use (2-2,5 lactation) and decreased fertility of breeding stock (67-72% – in the farms with intensive-industrial technologies). At high intensity of herd selection – 30-33%, it is practically impossible to obtain the expanded reproduction of a herd from its own resources. Therefore, it always has to depend on the import of heifers and feeder heifers. These circumstances may necessitate the development of special breeding activities which eliminate these problems.
The science developed technology and laboratory equipment for sexed bull sperm and use of frozen-thawed sexed sperm relatively recently. Production test confirmed high efficiency of separation of spermiums by sex (bulls: heifers) – up to 92%. However, the high cost of sexed sperm and reasonable doubts of scientists and geneticists on the biological "harmlessness" of the proposed technology require further research in the future.
The aim of our work was to conduct a comparative assessment of sexed and traditional sperm quality of the same Holstein bulls, which comes in straws and proposed for using in farms of Ukraine.
Sexed and traditional (not sexed) sperm of Holstein sires of Canadian selection from "Simex Alliance Ukraine" LTD was used for research. The sperm of 4 sires: Benjamin CANM 7866444, Ardent HOUSAM 137922325, Mathys CANM 103439288, Vioris Sleeman HOCANM7817774 was taken for the analysis; each of the bulls had 3 sexed sperm doses and 3 traditional sperm doses. Total 24 sperm doses were studied.
Thaw-frozen bull sperm was studied in the cryopreservation laboratory SPC "Zahіdplemresursy" Ltd., Lviv region using the technological equipment of the German company «Minitube» according to the software package CASA (Computer Assisted Semen Analysis) – Sperm Vision. Assessment of semen quality was conducted on indicators: concentration of sperms in 1ml, motility after thawing, number of sperms with rectilinear reciprocating movement (RRM), circular motion and stationary, and after incubation at 37 ° C after 60, 120, 180 minutes; acrosome intactness, level of microbial contamination.
It has been established that motility and survivability of the sexed sperms were15-20% lower compared with these indicators of traditional sperm. Irrespective of the division of sperm by sex, we had the highest activity of the sperms of bull Vioris Sleeman HOCANM7817774. This indicates the possibility of bull selection by this indicator of quality sperm.
The experimental results should not be assessed pessimistically. The similar problems occurred at the early stages of development and adopting of freezing and thawing technology of native bull sperm. We know that these issues have been successfully resolved.
Therefore, the experimental results indicate necessity of improving the technology of freezing and thawing sexed bull sperm and preparing specialists of required qualification for the breeding centres laboratories and breeding enterprises in Ukraine.
The genotype of a number of generations of progeny, obtained using sexed sperm should be systematically studied in the future.
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Swain, Dilip Kumar, Prasant Swarnkar, Jitender Kumar, and Sarvajeet Yadav. "Evaluation of DNA integrity of caprine cauda epididymal spermatozoa." Indian Journal of Animal Sciences 82, no. 8 (August 14, 2012): 854–55. http://dx.doi.org/10.56093/ijans.v82i8.23008.
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Sperm DNA integrity of the sperms recovered from cauda epididymis was evaluated by Comet assay to study the sperm DNA integrity and viability of the sperms through highly efficient fluorescent staining. With the advancement of the storage time, the viability and intactness of the sperm DNA got compromised. Our findings will strongly form a baseline data for further studies about the cauda epididymal sperms in terms of DNA integrity. We reported that cauda can be a source of retrieval of sperms for assisted reproduction after the death of the animal. We strongly argue for the development of suitable extenders with antioxidants for the reduction of free radical mediated damage to the sperms as well as optimal cryopreservation technique. The sooner the recovery of the sperms from the cauda the better the viability and an increased probability for fertilisation. Further studies are required to elucidate the key cellular and molecular mechanisms which are associated with the loss of quality of the epididymal sperms.
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Raouf, Chro Ghafoor, and Mahmood Othman Ahmad. "Suspected Protective Effect of Eruca Sativa Leaves Extract on Sperm Abnormalities in Male Albino Mice Treated with Hypericum triqutrifolium aqueous crude extract." UHD Journal of Science and Technology 3, no. 2 (November 14, 2019): 87. http://dx.doi.org/10.21928/uhdjst.v3n2y2019.pp87-92.
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This study was carried out to evaluate the effect of Eruca sativa on the cytotoxic effect of Hypericum triquetrifolium on sperm abnormalities in albino mice. Leaves of E. sativa and aerial parts of H. triquetrifolium were dried in shade and grinded and their aqueous extracts were used for the treatments to study their effect on sperm morphology. Treated groups were injected with a single dose of 38 mg/kg body weight (BW) of Hypericum subcutaneously, while the Eruca groups were orally administered with 250 mg/kg BW twice/week for 2 weeks. After the exposure of H. triquetrifolium, the frequency of abnormal sperms showed a highly significant induction of sperm abnormalities; separated head from tail sperms, swollen head, hookless, defective head, and hook. However, the Eruca group showed no obvious abnormalities in sperm morphology, while in cotreatment with both Eruca and Hypericum (H/E) groups, there was an extremely significant decrease (P < 0.0001) in the abnormal sperms. In conclusion, it appeared that E. sativa could prevent or at least minimize the damages that Hypericum toxins would make on the sperm morphology significantly.
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Lokesh, G., Geetha N. Murthy, Veeranna Gowda, Alok Sahay, and Gargi Gargi. "Conservation of wild silkworm genetic resources through cryopreservation: Standardization of sperm processing." Journal of Applied and Natural Science 10, no. 2 (June 1, 2018): 544–47. http://dx.doi.org/10.31018/jans.v10i2.1733.
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Conservation of the invaluable sericigenous genetic resources is of prime importance with respect to their utilization and improvement for wider exploitation. Conservation of wild silkworms and its applicability in hybridization have limitations due to incompatibility, less amenability, change of behaviour under ex situ conditions, non-synchronization of moth eclosion and difficulties in mating between variables. In view of this, the newer technologies such as cryopreservation and artificial insemination are offering better strategies for preservation of biologically active samples like semen at sub-zero temperature (-196º C) conditions for longer duration. In this context, under standardization of sperms preservation from wild silkworms, two methods of semen collection were scrutinized for obtaining active and viable sperm for cryopreservation and further artificial insemination. Semen collection from the seminal vesicle of freshly emerged male moth and the other from the bursa copulatrix (BC) and spermatheca of the female moth after mating. The sperms in the semen collected from seminal vesicle are in the form of bundles known as eupyrene sperm bundles and apyrene sperms. The morphology and behaviour of these sperm bundles were recorded through microscopic examination. To study the density and motility behaviour of the sperms, sperm bundles were treated with proteolytic enzyme (~2-3µg/ml) to digest the membrane and release the sperms. The density and motility behaviour of sperms in the semen recovered from the BC and spermatheca of female moth after mating were higher compared to those released after digestion of sperm bundles from seminal vesicle of the male moth.
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Khodamoradi, Maedeh, Saeed Rafizadeh Tafti, Seyed Ali Mousavi Shaegh, Behrouz Aflatoonian, Mostafa Azimzadeh, and Patricia Khashayar. "Recent Microfluidic Innovations for Sperm Sorting." Chemosensors 9, no. 6 (June 1, 2021): 126. http://dx.doi.org/10.3390/chemosensors9060126.
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Sperm selection is a clinical need for guided fertilization in men with low-quality semen. In this regard, microfluidics can provide an enabling platform for the precise manipulation and separation of high-quality sperm cells through applying various stimuli, including chemical agents, mechanical forces, and thermal gradients. In addition, microfluidic platforms can help to guide sperms and oocytes for controlled in vitro fertilization or sperm sorting using both passive and active methods. Herein, we present a detailed review of the use of various microfluidic methods for sorting and categorizing sperms for different applications. The advantages and disadvantages of each method are further discussed and future perspectives in the field are given.
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Belayeva, N. S. "Relation of the male gametes with embryo sec cells. The hypothesis of double fertilization." Acta Societatis Botanicorum Poloniae 50, no. 1-2 (2014): 173–75. http://dx.doi.org/10.5586/asbp.1981.026.
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The sperms one after another get out of synergids. The front sperm gets the first into the egg cell attraction zone and then the sperm comes into contact with egg membrane. At this moment Attraction ceases and the second sperm is led by a current of cytoplasma to the central nucleus. In the egg cell the sperm nucleus is led to the nucleus by cytoplasmic current too. After fertilization the character of cytoplasmic motion changes, because of a cell membrane damage. The presence of the sperm in the female nuclei may also serve as a regulating factor.
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Rahma, Magnadi Yogi, Wiryawan Permadi, Anita Rachmawati, Jusuf Sulaeman Effendi, Tono Djuwantono, and Budi Handono. "Hubungan Kadar Vitamin D Serum dengan Konsentrasi, Motilitas dan Morfologi Sperma pada Pria Subfertil di Klinik Teknologi Reproduksi Berbantu Aster Rumah Sakit Dr. Hasan Sadikin Bandung." Indonesian Journal of Obstetrics & Gynecology Science 3, no. 1 (March 29, 2020): 55. http://dx.doi.org/10.24198/obgynia.v3i1.89.
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Tujuan: Untuk menganalisis hubungan kadar vitamin D dengan kualitas sperma pada pria subfertil, serta membandingkan kualitas sperma berdasarkan klasifikasi kadar vitamin D serum pada pria normospermia dan pria oligoasthenoteratospermia.Metode: Penelitian ini merupakan penelitian yang bersifat observasional analitik dengan rancangan studi potong lintang. Subjek penelitian adalah pria subfertil yang berusia 20-50 tahun, dibagi menjadi 2 kelompok, yaitu 30 pasien normospermia dan 16 pasien oligoasthenoteratospermia berdasarkan berdasarkan kriteria World Health Organization 2010. Penelitian dilakukan di Klinik Teknologi Reproduksi Berbantu Aster, RSUP Dr. Hasan Sadikin Bandung. Analisis data menggunakan analisis korelasi rank SpearmanHasil: Pada penelitian ini didapatkan korelasi positif antara kadar vitamin D dan motilitas sperma ( r = 0,639, p = < 0,001), tetapi tidak didapatkan korelasi pada konsentrasi dan morfologi sperma ( p>0,05). Perbedaan yang bermakna didapatkan antara kadar vitamin D pada kelompok normospermia dan oligoasthenoteratospermia dengan nilai p<0,05. Pada perbandingan kualitas sperma berdasarkan klasifikasi kadar vitamin D hanya motilitas sperma saja yang memiliki perbedaan bermakna dengan nilai p<0,05.Simpulan: Terdapat hubungan yang bermakna antara kadar vitamin D serum dan motilitas sperma.Correlation Of Vitamin D Serum Level With Concentration, Motility, And Morphology Of Spermatozoa Subfertil In Assisted Reproductive Technology Aster Clinic Hasan Sadikin Hospital BandungAbstractObjective: This study aims to analyze the relationship of vitamin D levels with sperm quality in subfertile men, as well as comparing sperm quality based on vitamin D serum levels classification in normospermic men and oligoasthenoteratospermia men.Method: This study is an analytic observasional with cross sectional design. Research subjects were subfertile men aged 20-50 years divided into 2 groups, consisting of 30 patients with normospermic and 16 patients with oligoasthenoteratospermia based on World Health Organization 2010 criteria. The research was conducted at Assisted Reproductive Technology Aster, RSUP Dr. Hasan Sadikin Bandung. Data analysis was using Spearman’s rank correlation analytic.Result: We found a positive correlation between vitamin D levels and sperm motility (r = 0.639, p = <0.001), but no correlation was found on sperm concentration and morphology (p> 0.05). There was a significant difference between vitamin D levels in normospermic and oligoasthenoteratospermia group with p <0,05. In the sperm quality comparison based on vitamin D serum classification, only sperm motility alone had a significant difference with the value of p <0.05. It was concluded that level of vitamin D serum has positive correlation with sperm motility.Key word: subfertile, vitamin D, sperm quality
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Moazin, Maher Saleh, Muhammad Tahir Bashir Malik, Muhammad Aslam, Naif Aldaham, and Muhammad Yahya Alrawi. "MICROSCOPIC TESTICULAR SPERM EXTRACTION." Professional Medical Journal 25, no. 02 (February 10, 2018): 180–84. http://dx.doi.org/10.29309/tpmj/2018.25.02.439.
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Azoospermia, the complete absence of sperms in the ejaculate is found in 1% ofnormal males and 10-15% of infertile couples. Surgical sperm retrieval (SSR) and use of spermsfor ICSI/IVF offers an opportunity of parenting to the male factor infertile couples. Micro-TESEgives higher surgical sperms retrieval rates in those patients in whom the chances of spermretrieval otherwise are very low. Objectives: To evaluate the outcome of Microscopic TesticularSperm Extraction (Micro-TESE) in different patients groups of non-obstructive azoospermia,in terms of testicular volume, histopathology, hormones levels as well as cytogenetic variants.Study Design: Retrospectively reviewed. Setting: Urology Division, King Fahd Medical City,Riyadh in collaboration with King Abdullah Reproductive Medical Unit (RMU). Period: January2011 to January 2016. Material & Methods: Fifty-four patients of primary and secondary infertilityage range of 29 to 65 years who had undergone Microscopic Testicular Sperm Extraction(Micro-TESE). Outcome measures: Finding of sperm in testicular specimen extracted bymicroscopic testicular dissection. Results: Out of 54 patients, hormonal abnormalities werefound in 45% patients and 65% had low volume testes. Abnormal histology was found in 23%patients and 9% had chromosomal abnormalities. Overall sperm retrieval rate in all groups was33%. Sperm retrieval rate was 34 % in patients with small volume testes (<15ml), 42% patientswith abnormal hormones (FSH), 33% patients with abnormal histology and 38% in patientswith chromosomal abnormalities. Minor complications (small hematoma and orchalgia) wereobserved in two (3.7%) patients. Conclusion: Micro-TESE is a valid option for sperm retrieval inpatients in which probability of sperm retrieval is otherwise very low.
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Sachinkumar Sahebrao Patil. "Ayurvedic management of male Vandhyatwa with special reference to male infertility." World Journal of Advanced Research and Reviews 15, no. 1 (July 30, 2022): 694–700. http://dx.doi.org/10.30574/wjarr.2022.15.1.0742.
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Infertility is the not getting pregnant, despite of having carefully timed, unprotected sex for one year.Various environmental and biological factors affect the sperm count in males. Oligozoospermia means low sperm count and Azoospermia means complete absence of sperms is one of the main cause of male infertility and term can be correlate with KsheenaShukra andNirbeeja /Ashukra respectively. There is no satisfactory treatment in modern medicine for this condition. Asthenozoospermia means the motility of spermatozoa is lower than 50% of active motile sperm. Ayurveda has many ways to increase sperm count, sperm motility and thus increase fertility. Sperm quality, quantity and motility decreases as their age progresses. The Aging process can be slow down by using Ayurvedic Vajeekarana Chikitsa (Aphrodisiac Therapy) which rejuvenates the male reproductive system and slows down the aging process also increases the quality, quantity and motility of sperms . It has also recommended an appropriate use of plant based remedies, a proper lifestyle and nutritious diet, Panchakarma,Yoga for improving overall health and treating male infertility. Current paper aims at putting forward the causes and treatment of male infertility as described in Ayurveda.
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Dobrovolny, Michal, Jakub Benes, Jaroslav Langer, Ondrej Krejcar, and Ali Selamat. "Study on Sperm-Cell Detection Using YOLOv5 Architecture with Labaled Dataset." Genes 14, no. 2 (February 9, 2023): 451. http://dx.doi.org/10.3390/genes14020451.
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Infertility has recently emerged as a severe medical problem. The essential elements in male infertility are sperm morphology, sperm motility, and sperm density. In order to analyze sperm motility, density, and morphology, laboratory experts do a semen analysis. However, it is simple to err when using a subjective interpretation based on laboratory observation. In this work, a computer-aided sperm count estimation approach is suggested to lessen the impact of experts in semen analysis. Object detection techniques concentrating on sperm motility estimate the number of active sperm in the semen. This study provides an overview of other techniques that we can compare. The Visem dataset from the Association for Computing Machinery was used to test the proposed strategy. We created a labelled dataset to prove that our network can detect sperms in images. The best not-super tuned result is mAP 72.15.
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Fikriyah, Siti, and Ninis Trisyani. "Efektivitas Penambahan Bahan Pengencer Sperma Yang Berbeda Terhadap Tingkat Keberhasilan Ginogenesis Ikan Mas Punten (Cyprinus carpio L.)." Jurnal Ilmu-ilmu Perikanan dan Budidaya Perairan 18, no. 2 (December 25, 2023): 113–18. http://dx.doi.org/10.31851/jipbp.v18i2.9629.
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Ginogenesis merupakan proses terbentuknya zigot dari gamet betina tanpa kontribusi dari gamet jantan. Manfaat melakukan ginogenesis adalah dapat mempercepat proses pemurnian, membuat populasi klon hanya dalam dua generasi, membuat populasi tunggal kelamin betina, mempercepat proses dan mendeterminasi genotip jenis kelamin betina. Penelitian ini bertujuan untuk mengetahui efektivitas penambahan bahan pengencer sperma yang berbeda terhadap tingkat keberhasilan ginogenesis ikan mas punten (Cyprinus carpio L.). Pada penelitian ini menggunakan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dengan 3 pengulangan, yaitu Sperma + 100% NaCl; Sperma + 50% madu + 50% NaCl; Sperma + 50% Air Kelapa Muda + 50% NaCl; Sperma + 50% Sari Tebu + 50% NaCl. Perlakuan terbaik yang dihasilkan dalam penelitian ini adalah sperma yang ditambahkan dengan air kelapa muda dan NaCl dengan nilai motilitas 83,33%, FR 48%, HR 40,5%, SRe 95,82%, SR-7 91,74%, dan SR-21 85,55%. Gynogenesis is the process of forming a zygote from female gametes without the contribution of male gametes. The benefits of gynogenesis are that it can speed up the purification process, create a clone population in only two generations, create a single female population, speed up the process and determine the genotype of the female sex. This study aimed to determine the effectiveness of adding different sperm diluents to the success rate of gynogenesis of punten carp (Cyprinus carpio L.). The method used in this study is an experimental research method using a completely randomized design (CRD) consisting of 4 treatments with 3 repetitions, namely P1: Sperm + 100% NaCl, P2: Sperm + 50% honey + 50% NaCl, P3: Sperm + 50% Young Coconut Water + 50% NaCl, P4 : Sperm + 50% Sugarcane Juice + 50% NaCl. The results obtained in this study were sperm dilution which significantly affected motility, fertilization rate, hatching rate, embryo survival rate, survival rate – 7 days, and survival rate – 21 days. The addition of young coconut water became the best treatment with motility value of 83.33%, FR: 48%, HR: 40.5%, Sre: 95.82%, SR-7: 91.74%, and SR-21: 85, 55%.
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almana, Yahya, and Ula AL-kawaz. "Comparison in Pregnancy Rates After ICSI Using Fresh Sperm Versus Cryopreserved." IraQi Journal of Embryos and Infertility Researches 12, no. 2 (November 6, 2022): 81–91. http://dx.doi.org/10.28969/ijeir.v12.i2.r6.22.
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For couples with a variety of male factor disorders, intracytoplasmic sperm injection has made pregnancy and motherhood possible. Two hundred-one males who had non-obstructive azoospermia or severe oligospermia were included in this study. One hundred forty trials of ICSI were done by using fresh sperms (fresh group n=140), while 61 trials of ICSI were done by using cryopreserved sperms (cryopreserved group n=61) to compare the conception rates between fresh and cryopreserved sperms used in intracytoplasmic sperm injection (ICSI). Twenty-five samples from each group were collected by doing a testicular biopsy. Pregnancy rates were higher using cryopreserved sperms as compared to fresh spermatozoa, 39.3% compared to 24.3% in the fresh group (P .030), respectively. Binary logistic regression analysis showed that the odds ratio of achieving pregnancy with cryopreserved sperms was 2.022 higher than with fresh (P .030). Cryopreservation has no negative effect on the pregnancy rate. Instead, it might improve the results of ICSI in comparison with a fresh sperm sample.
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Mohamed, Amal, Aza Hassan, Magdy Amer, and El-Said Abdel-Aziz. "The effects of oral ketoconazole and griseofulvin on the fertility of male rabbits." Mansoura Veterinary Medical Journal 21, no. 2 (June 25, 2020): 32–38. http://dx.doi.org/10.35943/mvmj.2020.21.2.0203.
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Objectives: To investigate possible side effects of ketoconazole and griseofulvin on fertility of clinically healthy male rabbits. Design: Randomized controlled experimental study. Animals: Thirty mature male rabbits. Procedures: Rabbits were randomly allocated into three equal sized groups (10 animals each). The first group served as a control group (received no treatment), the second group received griseofulvin (25 mg/kg B.W) orally for 30 days, and the third group received ketoconazole (50 mg/kg B.W) orally for 30 days. Semen samples were collected after 1 day, 1 week and 2 weeks post-administration to determine sperm motility, % of live and dead sperms, total sperm abnormalities and sperm cell concentration. Two weeks post-administration, testes and epididymides were removed for histopathological examination. Results: Both drugs produced a significant reduction in the serum testosterone level, sperm cell concentration, percent of live sperms and percent of sperm motility. Conclusion and clinical relevance: Ketoconazole and griseofulvin have a negative impact on fertility of male rabbits, and the effect is more pronounced with ketoconazole.
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Ragab, Mohamed Wael, Mina Saad, Zeinab Nour, Hamed Abdallah Hamed, Taymour Mostafa, and Ahmed M. El-Guindi. "Seminal TEX101 May Predict Seminal Sperm Recovery after Varicocelectomy in Nonobstructive Azoospermic Patients with Varicocele." Andrologia 2023 (September 22, 2023): 1–6. http://dx.doi.org/10.1155/2023/5652572.
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Background. Infertile men with nonobstructive azoospermia (NOA) associated with varicocele may benefit from varicocele repair with recovered sperms in semen. Currently, there is no clinically applied method to predict the success of seminal sperm recovery in this subset of azoospermic men. Objective. We aimed to evaluate the predictive role of the seminal testis expressed protein (TEX101) in sperm recovery in the semen of NOA men with palpable bilateral varicocele after microsurgical varicocelectomy. Patients and Methods. This prospective cross-sectional cohort study included 40 patients with NOA and bilateral varicocele. Seminal TEX101 levels were measured followed by subinguinal microsurgical varicocele repair. Two seminal analyses were carried out at 3- and 6-month follow-up periods to evaluate seminal sperm recovery. Results. After varicocele repair, sperms were recovered in the semen of 10/40 patients (25%) along the follow-up periods (seven patients after 3 months and additional three patients after 6 months). The preoperative median seminal TEX101 level was significantly higher in NOA men with seminal sperm recovery compared with NOA patients without seminal sperm recovery (13.5 vs. 9.8 ng/ml, p = 0.014 ). Besides, the serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) median levels were significantly higher in NOA men without seminal sperm recovery compared with NOA men with seminal sperm recovery ( p = 0.001 , p = 0.01 ). There were nonsignificant differences comparing the preoperative testicular volume or serum testosterone levels between the two investigated groups ( p = 0.072 , p = 0.272 ). A cutoff value of 9.9 ng/ml was demonstrated to have preoperative TEX101 prediction of seminal sperm recovery (sensitivity of 90% and specificity of 57%). Conclusion. Preoperative seminal TEX101 could be considered as a biomarker for sperm recovery for seminal sperm recovery in NOA cases associated with varicocele after its surgical repair. Further work at this point with larger number of cases should be carried out to verify its potential importance.
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Jonáková, Věra, and Marie Tichá. "Boar Seminal Plasma Proteins and Their Binding Properties. A Review." Collection of Czechoslovak Chemical Communications 69, no. 3 (2004): 461–75. http://dx.doi.org/10.1135/cccc20040461.
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Binding properties of a group of proteins isolated from boar seminal plasma and their role in the fertilization process are discussed. Boar seminal plasma contains different types of proteins: spermadhesins of AQN and AWN family, DQH and PSP proteins belong to the most abundant. Some of these proteins are bound to the sperm surface during ejaculation and thus protein-coating layers are formed. Sperms coated with proteins participate in different types of interactions in the following steps of the fertilization process: formation of oviductal sperm reservoir, sperm capacitation, oocyte recognition and sperm binding. Saccharide-based interactions of boar seminal plasma proteins play role in the binding of sperm to oviductal epithelium, in sperm capacitation and primary binding of sperm to zona pellucida. An interaction with phospholipid components is responsible for the protein adsorption to sperm membrane. Interactions between proteins participate in the arrangement and remodelling of sperm-coating layers. Study of boar seminal plasma proteins, their characterization and elucidation of their interactions will contribute to understanding the fertilization process. A review with 82 references.
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Al-Dulimi, Ayman Ali H., and Ali Abdullah Zuairi Al-Sadoon. "Effect of Aqueous Extract of Dill leaves (AEDL) on some Traits of Ram Epididymal Sperms under Cooling Conditions." IOP Conference Series: Earth and Environmental Science 1262, no. 7 (December 1, 2023): 072048. http://dx.doi.org/10.1088/1755-1315/1262/7/072048.
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Abstract The current study was conducted in the Graduate Studies Laboratory of the College of Agriculture, Al-Muthanna University, for the period from December 3, 2022 to March 9, 2023, to determine the effect of an aqueous extract of dill leaves ( AEDL)on some properties of epididymal sperms of rams under cooling conditions of 5 °C for different periods. The aqueous extract of dill leaves was prepared and kept in the refrigerator until the time of use. The epididymal sperms were obtained from the testis of Rams that slaughters in the local slaughterhouse of Al-Muthanna Governorate, the testicles were transferred to the laboratory less than an hour after the slaughter of the animal, the caudal epididymis was diluted with a Tris diluent containing 3% concentration of aqueous extract of dill leaves. The results of using the aqueous extract of dill leaves showed a significant increase (P<0.01) in the percentage of live sperm, Individual motility, and a decrease in the percentage of abnormal sperms. While the use of the extract did affect the percentage of the integrity of acrosome represented by the hypo osmotic swelling test (HOST) at the 0 time of cooling preservation, The Increasing of the time of sperm cooling.leads to a decreases in the percentage of live sperm, HOST, individual motility, with an increase in the percentage of abnormal sperms. The use of the aqueous extract of dill leaves at different times resulted in a significant effect(P< 0.01) on the percentage of live sperm, individual motility, while it did not significantly affect the percentage of abnormal sperm, and there were no significant differences in the percentage of HOST. according to the results of this study We can conclude to adding the aqueous extract of dill leaves to Improve the physical traits of rams epididymal sperm include the live sperm and individual motility under cooling conditions for different periods.
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Takea S. A. "Protective effect of Ginkgo biloba and Olive oil on the reproductive performance of male albino rats exposed to oxidative stress induced by Potassium dichromate." Tikrit Journal of Pure Science 22, no. 8 (February 1, 2023): 94–102. http://dx.doi.org/10.25130/tjps.v22i8.857.
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The present study is designed to demonstrate the effect of Ginkgo biloba and Olive oil on the reproductive performance of male rats exposed to oxidative stress induced by Potassium dichromate with respect to male sex hormones, semen parameters and testicular antioxidant system,. Forty Wister albino rats were distributed into four groups and each group have ten rats, control group, Potassium dichromate group (Potassium dichromate 24 mg/kg administered with gavage for 2 month), Potassium dichromate treated with Ginkgo biloba group (Potassium dichromate and Ginkgo biloba 40 mg/kg administered at half an hour interval for 2 months) and Potassium dichromate treated with olive oil group (Potassium dichromate and Olive oil 1 ml/kg administered at half an hour interval for 2 months). The statistical analysis of results showed a significant (P<0.05) decrease in sperms count, the percentage of sperms viability, the level of sex hormones, ceruloplasmin, glutathione concentration, and superoxide dismutase activity, antioxidant activity (AOA), germinal epithelium height and diameter of seminiferous tubules and a significant increase of sperm percentage abnormalities, dead sperm, malondialdelyde (MDA), peroxynitrite (ONOO-) in Potassium dichromate group, compared with control group. The administration of Potassium dichromate with Ginkgo biloba and Olive oil separately result in a significant increase of sperms count, sperms viability, sex hormones levels, ceruloplasmin, glutathione concentration , superoxide dismutase and antioxidant activity, germinal epithelium height and diameter of seminiferous tubules and a significant decrease of sperm abnormalities, dead sperm percentage, MDA, ONOO-. In conclusion the levels of antioxidant parameters, sperm count and sperm viability were restored to normal values when Potassium dichromate administered in combination with Ginkgo biloba or with Olive oil therefor Ginkgo biloba and Olive oil may be beneficial for improving reproductive performance of male rats.
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Mahour, Simmi, and Sreelakshmy R. Nair. "Fertility outcomes in microdissection testicular sperm extraction for non-obstructive azoospermia." International Journal of Reproduction, Contraception, Obstetrics and Gynecology 12, no. 12 (November 28, 2023): 3604–9. http://dx.doi.org/10.18203/2320-1770.ijrcog20233642.
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Background: The objective of this study was to evaluate outcome of micro dissection testicular sperm extraction (micro TESE) and intracytoplasmic sperm injection (ICSI) for treatment of non-obstructive azoospermia (NOA). Methods: We retrospectively analysed data of 96 consecutive patients with clinical NOA who were treated with micro TESE by single surgeon, between January 2022 and December 2022, in Lifeline superspeciality hospital Adoor, Kerela. Embryological and clinical outcomes were demonstrated based on ICSI-IVF cycles using fresh or frozen sperms, different etiologies of NOA and various counts of sperms retrieved. Results: 96 men underwent micro TESE and 72.9% (70/96) of them had sperms retrieved. ICSI performed in 64 couples. Of those, 41 reached the stage of embryo transfer (ET). Of the couples who underwent embryo transferred, 18 (43%) resulted in biochemical pregnancies and 7 (17%) clinical pregnancies. There was a significant difference in the testicular volume and serum FSH levels between micro-TESE positive and negative groups (p=0.000). Retrieval rates were higher in group of men with normal testicular volume and FSH<12. Clinical pregnancy rate was around 11% in couples who had sperms retrieved by micro TESE. The sperm retrieval rates were higher in men with age <40 years. Similarly younger the female age more was the pregnancy rate. Out of various etiologies idiopathic NOA and Klinefelter syndrome had better sperm retrieval rate in our study which was statistically significant. Total fertilization rate and blasts rates were 79.7% and 51.6% out of ICSI. Conclusions: Microdissection testicular sperm extraction is an effective treatment for NOA with higher rate of sperm retrieval and pregnancy rate. The increasing success rates over several years indicate the importance of surgical skill and laboratory staff experience.
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Ahuja, Meenu Vashisht, and Pankaj Talwar. "A successful outcome in a case of nonobstructive azoospermia with repeat surgical sperm retrieval (SSR)-testicular sperm aspiration followed by microsurgical testicular sperm extraction." Fertility Science & Research 10, no. 4 (October 2023): 219–22. http://dx.doi.org/10.4103/fsr.fsr_34_23.
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Azoospermia is a major cause of male infertility, either obstructive or nonobstructive azoospermia (NOA). However, such couples should be offered surgical sperm retrieval to enable them to become parents with their own gametes. Cases of NOA will have higher retrieval rate with microsurgical testicular sperm extraction (micro-TESE), even though, in some cases, it may be a repeat procedure. Presenting here a case of successful twin gestation after intracytoplasmic sperm injection with sperms retrieved successfully by micro-TESE after a previous failed frozen embryo transfer with a very poor retrieval by a testicular sperm aspiration in a case of NOA.
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Pérez-Marín, Carlos Carmelo, Ander Arando, Francisco Maroto-Molina, Alberto Marín, and Juan Vicente Delgado. "Las subpoblaciones de espermatozoides y su calidad en fracciones producidas por la centrifugación de una sola capa en muestras frescas y normospérmicas de esperma de cordero." Revista Mexicana de Ciencias Pecuarias 12, no. 2 (September 15, 2021): 386–401. http://dx.doi.org/10.22319/rmcp.v12i2.5683.
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Single layer centrifugation (SLC) technique has been developed to select the best sperm population in the ejaculate in order to increase the fertilization rates by artificial insemination or in vitro fertilization. Normospermic ram semen samples containing 800 and 3,000 × 106 sperms/ml (C800 and C3000, respectively) were processed by SLC. Three sperm fractions were separated in each sample following silica-coloidal sperm centrifugation and sperm yield, quality and subpopulations were analyzed in each one. In C800 group, the sperm recovery rate did not vary in any studied fraction, but when samples were highly concentrated (C3000) the top fraction (F1) contained significantly higher spermatozoa than bottom fraction (F3). Also, it was observed that F1 in C3000 had got a significantly higher percentage of spermatozoa (53.2 %) than in C800, while the quantity of spermatozoa recovered in fraction 2 was lower (25.2 % vs 45.4 %). Based on the sperm motility parameters, three sperm subpopulations were identified: SP1, low velocity spermatozoa showing no progressive movement (19.1 %); SP2, rapid and progressive spermatozoa (43.7 %); and SP3, rapid spermatozoa but non-linear movement (37.2 %). While SLC has been implemented for sperm separation in suboptimal and/or low concentrated sperm samples, this trial demonstrates that SLC is not efficient to separate different sperm populations in normospermic ram sperm samples containing high concentrations of spermatozoa.
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Cho Ping, Ng, Noor Hashida Hashim, and Durriyyah Sharifah Hasan Adli. "Effects ofNigella sativa(Habbatus sauda) Oil and Nicotine Chronic Treatments on Sperm Parameters and Testis Histological Features of Rats." Evidence-Based Complementary and Alternative Medicine 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/218293.
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Twenty-fourSprague-Dawleymale rats (7–9 weeks old, 200–250 g) were divided into Nicotine (N) (0.5 mg/100 g body weight (BW), Nicotine Control (NC) (saline, 0.1 mL/100 g BW),Habbatus saudaoil (HS) (6.0 μL/100 g BW), andHabbatus saudaControl (HSC) (corn oil, 0.1 mL/100 g BW) groups and treated for 100 days. Sperm parameters and seminiferous tubules measurements were evaluated. The N showed a significantly lower sperm motility (1.03±0.05×106 sperm/mL) and percentage of normal (82.61±0.03%) and live (93.88±0.01%) sperm, higher value for the seminiferous tubule (253.36±1.83 μm) and lumen (100.15±2.38 μm) diameters and spermatogonia (19.85±0.39 μm) and spermatocytes (33.37±0.59 μm) layers, and thinner spermatid-sperm layer (22.14±0.71 μm) than the NC (P<0.05). The HS had significantly higher sperm motility (1.49±0.04×106 sperm/mL) and percentage of normal (90.61±0.01%) and live (96.98±0.01%) sperm, smaller lumen diameter (67.53±2.34 μm) and thinner spermatogonia (17.67±0.32 μm) and wider spermatid-sperm (36.95±0.79 μm) layers than the HSC (P<0.05). This research confirmed that nicotine reduced sperm motility and morphology of normal and live sperms and also affected the testis histology, whileHabbatus saudaoil increased sperm quality and gave better testis histological features.
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Khunkaew, Chakorn, Prapas Patchanee, Sasithorn Panasophonkul, Zubing Cao, Zhang Yunhai, and Anucha Sathanawongs. "The sperm longevity and freezability in the modified BHSV extender of Thai Pradu-hangdum chicken." Veterinary Integrative Sciences 19, no. 2 (December 28, 2020): 161–72. http://dx.doi.org/10.12982/vis.2021.015.
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Pradu-hangdum is a distinctive Thai native pure breed chicken. To conserve the pure breed of this chicken species, the artificial insemination was invested. The key to the highly successful output of this technique is high quality of semen. Hence, the objective of the present study is to characterize the Pradu-hangdam sperms and their longevity and freezability in the BHSV extender. The semen of the 30 randomized male chicken were collected. The macroscopic and microscopic examination were used to determine the sperm characters. The results revealed that the Pradu-hangdam sperms contained normal a spiral-shaped head, mid-piece and tail and normal white cream color. The mean number of sperm concentration was 5.24×109 ± 1.54 sperms/mL. The mean volume was 0.22 ± 0.08 mL. The results of longevity in mean total motility of sperm was 85.20%, 56.00% and 36.33% storage for 1, 24, and 48 hours after semen collection in extender, respectively. The longevity of sperm storage in extender decreased significantly at 24 and 48 hours (P<0.05). The freezability of Pradu-hangdam semen significant decreased from 81.45% to 57.02% of motility (P<0.05) but in the range of acceptable result for insemination. In conclusion, this study provides the basic knowledge of sperm characters and their longevity which decreases in relation to the time after collection even though it was preserved and frozen in the BHSV of the acceptable data. Furthermore, the freezing technique and fertility rate should be a further study in the long-term preservation of Pradu-hangdam sperms.
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Bonyadi, F., S. Hasanzadeh, H. Malekinejad, and G. Najafi. "Cyclopiazonic acid decreases sperm quality and in vitro fertilisation rate in mice." World Mycotoxin Journal 11, no. 4 (December 7, 2018): 599–610. http://dx.doi.org/10.3920/wmj2018.2337.
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The presence of cyclopiazonic acid (CPA) as a mycotoxin has been reported in feed and foodstuffs. The aim of this investigation was to determine the effects of CPA on reproductive functions of male mice. In this experiment, 40 mature male mice were randomly assigned into five groups (n=8): control, control-sham, CPA (0.03 mg/kg, body weight (BW)), CPA (0.06 mg/kg, BW) and CPA (0.12 mg/kg, BW). Following 28 days exposure to CPA, sperm quality parameters, in vitro fertilisation (IVF) capacity of sperms, serum testosterone level, Leydig cells number and serum total antioxidant capacity (TAC) were analysed. The results revealed a significant (P<0.05) reduction in sperm count, sperm viability, sperm motility, chromatin quality of sperm, sperms with intact DNA, IVF rate, testosterone level, Leydig cell distribution and TAC in comparison to the control group. The most prominent detrimental effects of CPA were found at the highest given dose level. Our results suggest that CPA at higher dose levels exerts detrimental effects on the male reproductive system. Moreover, these descriptive warrant further investigations into the specific mechanisms of action and the effects of CPA on spermatogenesis.
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Duc, Nguyen Huu, Pham Thu Giang, Tran Thi Binh Nguyen, Nguyen Thi Mai, and Bui Dai Phong. "Evaluating the ability of semen cryopreservation of Blance Blue Belge bull cattle in Vietnam." Vietnam Journal of Biotechnology 19, no. 2 (August 2, 2021): 237–44. http://dx.doi.org/10.15625/1811-4989/16332.
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The objective of this study was to determine the semen cryopreservation capacity of BBB bulls in Hanoi-Vietnam. Research conducted on the fresh semen collected from 05 BBB bulls. Results showed that semen color was normal (milky white, ivory white, ivory yellow), semen volume ranged from 6.35 mL to 7.48 mL (P <0.05), initial motility of semen ranged from 80.53% to 82.92% (P <0.05), sperm concentration in semen ranged from 1.02 x 109 sperms/ml to 1.12 x 109 sperms/mL (P <0.05), abnormal sperm ratio ranged from 6.45% to 8.12% (P <0.05), alive sperm ratio ranged from 76.34% to 82.97% (P <0.05), sperm motility after thawing from straw semen ranged from 71.33% to 75.92% (P<0.05). In conclusion, successfully semen collection from 05 breeding BBB bulls at Hanoi Cattle Breeding Joint Stock Company, semen samples had normal color and good quantity and quality, suitable for production of frozen semen; and semen cryopreservation of straws of the 05 bull BBB semen mentioned at -196oC, sperm motility after freezing-thawing reached the economic and technical norms of 675/2014 of the Ministry of Agriculture and Rural Development.
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Toropov, V. A., S. Yu Borovets, S. Kh Al’-Shukri, A. M. Gzgzyan, V. Ya Belousov, and I. D. Fedorova. "Endocrine predictors of testicular biopsy efficacy in patients with azoospermia." Grekov's Bulletin of Surgery 176, no. 3 (June 28, 2017): 38–42. http://dx.doi.org/10.24884/0042-4625-2017-176-3-38-42.
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OBJECTIVE. The study identified predictors of endocrine detection of sperm in case of open testicular biopsy in patients with non-obstructive azoospermia. MATERIAL AND METHODS. The research was based on the results of examination and testicular biopsy of 76 patients with secretory azoospermia aged from 20 to 55 years old. The instrumental, laboratory, physical methods of research were carried out for all the patients before performing of the open biopsy for testicular sperm extraction. Hormone levels were determined in blood plasma such as luteinizing hormone, follicle stimulating hormone, prolactin, total and free testosterone, estradiol and sex steroid binding globulin. Patients were divided into two groups. The first group consisted of patients (n =43) whom sperms were found in testicular biopsies. The second group included patients (n = 33) whom sperms weren’t detected. RESULTS. Сonсentration of follicle stimulating hormone in blood plasma was the most significant hormone predictor. It was found that follicle stimulating hormone level between 12 and 16 IU/L indicated to the low probability of sperm presence in testicular biopsies and in case it was less than 17 IU/L the probability was extremely low. There was also established that elevated follicle stimulating hormone levels in patients older than 26 years pointed to the low probability of finding sperm using open testicular sperm extraction. CONCLUSIONS. Increase of luteinizing hormone, follicle stimulating hormone or their correlation in blood plasma indicates to a low probability of finding sperm by open testicular sperm extraction. The concentrations of testosterone, sex steroid binding globulin, prolactin and estradiol in the blood plasma weren’t reliable predictors of finding sperm in testicular biopsies.
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Yulianti, Retno. "Efek Protektif Zink Terhadap Stres Oksidatif Testis dan Kualitas Sperma Pada Mencit Jantan (Mus Musculus) Setelah Diinduksi Cyclophosphamide." Jurnal Biosains Pascasarjana 22, no. 2 (December 1, 2020): 63. http://dx.doi.org/10.20473/jbp.v22i2.2020.63-72.
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AbstractCyclophosphamide is a class of alkylating agents used for cancer treatment. The side effect of CP is through the depletion mechanism of the GSH cellular pathway which generates free radicals by activation by copper/iron in the body to damage the spermatogenesis process. Zinc has antioxidant enzymes and can bind metallothionein to protect the body from the effects of anti-cancer drugs. The purpose of this study was to determine the protective effect of Zn against testicular oxidative stress and sperm quality in male mice (Mus musculus) after cyclophosphamide induction. This study used 30 male mice divided into six groups, namely negative controls that were not given CP and Zn (K1), positive controls that were given only Zn (K2) and only CP (K3) and groups that were given CP injections (200 mg / Kg) with 3 doses of Zn, namely 25 mg (K4), 50 mg (K5) and 100 mg (K6). The analysis showed that the mean sperm quality in all groups was significantly different (p=0.011). The mean levels of MDA testis in all groups did not differ significantly. There was no relationship between testicular MDA levels with sperms quality (p=0.800) and the degree of correlation in a negative direction (R= -0.048). This study concluded that testicular MDA levels are not associated with sperms quality, especially the number of sperms cells. Keywords : cyclophosphamide, malondialdehid, sperms, zinc AbstrakCyclophosphamide (CP) merupakan golongan alkylating agent yang digunakan untuk pengobatan kanker. Efek samping CP melalui mekanisme deplesi jalur seluler GSH yang menghasilkan radikal bebas dengan aktivasi oleh tembaga atau besi dalam tubuh sehingga merusak proses spermatogenesis. Zink (Zn) memiliki enzim–enzim antioksidan dan mampu mengikat metallothionein untuk melindungi tubuh dari efek obat anti kanker. Tujuan penelitian ini untuk mengetahui efek proteksi zink terhadap stress oksidatif testis dan kualitas sperma pada mencit jantan (Mus musculus) setelah diinduksi cyclophospamid . Penelitian ini menggunakan 30 mencit jantan yang dibagi enam kelompok yaitu kontrol negatif yang tidak diberi CP dan Zn (K1), kontrol positif yang hanya diberi Zn (K2) dan hanya CP (K3) serta kelompok yang diberikan penyuntikan CP (200 mg/Kg) dengan 3 dosis Zn yaitu 25 mg (K4), 50 mg (K5) dan 100 mg (K6). Hasil analisis menunjukkan rerata kualitas sperma (jumlah sel sperma) pada semua kelompok berbeda siginifikan (p=0.011). Rerata kadar MDA testis pada semua kelompok tidak berbeda signifikan. Tidak terdapat hubungan antara kadar MDA testis dengan kualitas sperma (jumlah sel sperma) (p =0.800, p>0.05) dan derajat korelasi ke arah negatif (nilai R= -0.048) Kesimpulan penelitian ini bahwa kadar MDA testis tidak berhubungan dengan kualitas sperma terutama jumlah sel sperma. Kata Kunci: cyclophosphamide, malondialdehid, sperma, zink
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Ding, Qiang, Xiuhu Ding, Shuwen Xia, Fang Zhao, Kunlin Chen, Yong Qian, Shaoxian Cao, et al. "Bta-miR-6531 Regulates Calcium Influx in Bovine Leydig Cells and Is Associated with Sperm Motility." Genes 13, no. 10 (October 3, 2022): 1788. http://dx.doi.org/10.3390/genes13101788.
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MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved in fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA in low-motility sperms in previous study. To investigate the mechanism of bta-miR-6531, 508 shared target genes of bta-miR-6531 were predicted using two miRNA target databases (TargetScan7 and miRWalk). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG), the calcium and cAMP signaling pathways were the most enriched of the target genes. A dual-luciferase assay indicated that bta-miR-6531 targeted ATP2A2 mRNA by binding to the coding sequence region. In bovine Leydig cells, bta-miR-6531 overexpression affected the intracellular calcium concentration by restraining ATP2A2 expression. Moreover, we observed high calcium concentrations and high ATP2A2 protein levels in high-motility sperm compared with those in low-motility sperms. Furthermore, high-linkage single-nucleotide polymorphisms (SNPs) of the pre-bta-miR-6531 sequence were identified that related to sperm traits. Genotype TCTC of bta-miR-6531 showed high sperm motility and density and low deformity rate in Holstein bulls. However, the mutation in pre-miR-6531 did not significantly affect mature bta-miR-6531 expression in the sperm or cell models. Our results demonstrate that bta-miR-6531 might involve in sperm motility regulation by targeting ATP2A2 of the calcium signaling pathway in bovine spermatozoa.
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Muchlisin, Zainal A., Dian Afriani, Kartini Eriani, Iwan Hasri, Firman M. Nur, Siti Maulida, Luvi S. Handayani, et al. "Improvement of Sperm Quality of the Depik Fish, Rasbora tawarensis, After Cryopreservation Using Antioxidant." Cryoletters 44, no. 1 (January 1, 2023): 13–19. http://dx.doi.org/10.54680/fr23110110312.
Full textAbstract:
BACKGROUND: The cryopreservation of the sperm of the depik fish, Rasbora tawarensis, has previously been developed. However, the quality of the sperm post cryopreservation was not satisfactory and might be improved through the application of antioxidants. OBJECTIVE: To determine the most suitable antioxidant for the cryopreservation of the depik fish spermatozoa. MATERIALS AND METHODS: A completely randomized design with a non-factorial experiment was used and the tested antioxidants were glutathione, β-carotene, ascorbic acid, and butylated hydroxytoluene (BHT) at 6% concentrations. All treatments had three replications. The sperms were collected from 10 male fishes and diluted with Ringer solution in a ratio of 1: 20 (v/v, sperm: Ringer solution). Then 5% DMSO and 5% egg yolk were added to the diluted sperms. Furthermore, 6% of the tested antioxidants were added to the diluents, and then, cryopreservation was carried out in liquid nitrogen for 14 days. RESULTS: The ANOVA test showed that the application of antioxidants significantly affected the sperm motility, fertility, and hatching rates of the eggs (P> 0.05). Furthermore, the antioxidants also protected the sperm cells during cryopreservation, with glutathione being the best antioxidant. CONCLUSION: The application of antioxidants during the cryopreservation of depik fish sperm had a significant effect on motility, fertility and hatchability of eggs post-cryo. Furthermore, glutathione was the most suitable antioxidant.
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Yang, Sijie, Xuan Gao, Taijian Zhang, Feifei Cai, and Haobo Zhang. "Density Gradient Centrifugation Alone or the Combination of DGC with Annexin V Magnetic-Activated Cell Sorting Prior to Cryopreservation Enhances the Postthaw Quality of Sperm from Infertile Male Patients with Poor Sperm Quality." Andrologia 2023 (April 4, 2023): 1–11. http://dx.doi.org/10.1155/2023/9030902.
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Objective. To examine whether density gradient centrifugation (DGC) alone or its combination with annexin V magnetic-activated cell sorting (DGC-MACS) can be used to process semen samples from infertile male patients with poor sperm quality prior to subjecting these to freeze/thaw process in order to optimize the outcomes of sperm freezing. Methods. This study enrolled sixteen patients with sperm concentration ≥ 20 × 10 6 / mL , sperm motility < 30 %, and/or <4% normal sperm morphology. Sperms were processed by DGC or DGC-MACS prior to the freeze/thaw process. Sperm motility, hyperosmotic swelling test (HOS), TUNEL test, and morphological analysis were performed before and after the freeze/thaw process. Results. The freeze/thaw process had a detrimental effect on sperm motility, viability, morphology, and DNA integrity in all three groups (RAW, DGC, and DGC + MACS groups). The DGC and DGC + MACS groups showed increased sperm motility, viability, and normal morphology following freeze/thaw than untreated frozen controls. The motility and viability were not significantly different between DGC-MACS-CPT (cryopreservation-thawing) and DGC-CPT groups. Moreover, almost no grade A or grade B sperm was observed in the DGC-MACS-CPT groups. The sperm selected by DGC or DGC + MACS showed decreased levels of sperm DNA fragmentation than RAW samples following freeze/thaw. Moreover, the sperm DNA fragmentation following freeze/thaw in the DGC-MACS-CPT group was significantly lower than that in the DGC-CPT group. Conclusions. Sperm preparation by DGC before cryopreservation improved the quality of sperm postthaw in infertile males with poor sperm quality. If the sperm quality following freeze/thaw is foreseen to be insufficient for artificial insemination with husband’s sperm or in vitro fertilization, or if there is high DNA fragmentation in RAW sperm, DGC + MACS should be used prior to cryopreservation to reduce sperm DNA fragmentation and improve the quality of sperm available for intracytoplasmic sperm injection.
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Mohammed A. Obid and Jawad Kadhim Arrak. "Protective Effect of Ellagic Acid on Epididymal Sperms Quality and DNA Deformity in Mature Rats Exposed to Cadmium Chloride." University of Thi-Qar Journal of agricultural research 12, no. 1 (June 8, 2023): 165–80. http://dx.doi.org/10.54174/utjagr.v12i1.247.
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This study was designed to investigate the protective effect of Ellagic Acid on Epididymal Sperms Quality and DNA Deformity in mature rats exposed to Cadmium Chloride. The experiment includes a total number of Sixty (60) male Albino Wistar rats weighting (180-220 g) were used in this experiment. Their ages ranged between (10-14) weeks. Rats were divided into four equal groups which administrated orally and daily for six weeks as follows: 100mg EA Kg B.wt, 5mg /Kg B.wt Cd, 100mg EA + 5mg Cd Kg B.wt, and tap water for group 1 (T1), group 2 (T2), group 3 (T3) and control group (C) respectively. The experiment was lasted for 6th weeks. Semen samples were collected after 2, 4 and 6 weeks for estimation sperms parameters. The results revealed a significant decrease of Sperm concentration and Sperms Motility in T2 as compared with control increase significantly of Sperm concentration and Sperms Motility in T1 and the results showed a significant increase of Sperms Abnormalities and Sperms Immotility in T2 with a significant decrease of Sperms Abnormalities and Sperms Immotility in T1 as compared with control. Depending on the current results and discussion, Conclusions are: Ellagic acid is an effective antioxidant against the toxic effect of Cadmium chloride on testicular function and Sperm Parameters.
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Diemer, Jorin, Jens Hahn, Björn Goldenbogen, Karin Müller, and Edda Klipp. "Sperm migration in the genital tract—In silico experiments identify key factors for reproductive success." PLOS Computational Biology 17, no. 7 (July 15, 2021): e1009109. http://dx.doi.org/10.1371/journal.pcbi.1009109.
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Sperm migration in the female genital tract controls sperm selection and, therefore, reproductive success as male gametes are conditioned for fertilization while their number is dramatically reduced. Mechanisms underlying sperm migration are mostly unknown, since in vivo investigations are mostly unfeasible for ethical or practical reasons. By presenting a spatio-temporal model of the mammalian female genital tract combined with agent-based description of sperm motion and interaction as well as parameterizing it with bovine data, we offer an alternative possibility for studying sperm migration in silico. The model incorporates genital tract geometry as well as biophysical principles of sperm motion observed in vitro such as positive rheotaxis and thigmotaxis. This model for sperm migration from vagina to oviducts was successfully tested against in vivo data from literature. We found that physical sperm characteristics such as velocity and directional stability as well as sperm-fluid interactions and wall alignment are critical for success, i.e. sperms reaching the oviducts. Therefore, we propose that these identified sperm parameters should be considered in detail for conditioning sperm in artificial selection procedures since the natural processes are normally bypassed in reproductive in vitro technologies. The tremendous impact of mucus flow to support sperm accumulation in the oviduct highlights the importance of a species-specific optimum time window for artificial insemination regarding ovulation. Predictions from our extendable in silico experimental system will improve assisted reproduction in humans, endangered species, and livestock.
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Kondracki, Stanisław, Anna Wysokińska, Magdalena Kania, and Krzysztof Górski. "Application of two staining methods for sperm morphometric evaluation in domestic pigs." Journal of Veterinary Research 61, no. 3 (September 26, 2017): 345–49. http://dx.doi.org/10.1515/jvetres-2017-0045.
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Abstract Introduction: The effect of two smear staining methods on the dimensions and shape of sperm cells in the semen of domestic pigs was evaluated. Material and Methods: The studies were carried out on 30 ejaculates collected from 15 boars, which included five Duroc boars, five Pietrain boars, and five hybrid Duroc × Pietrain boars. Each ejaculate was next sampled to make two microscopic slides, of which one was stained with eosin-nigrosin and the other with eosin-gentian dye. In total, 600 measurements of sperm cells were made. Each sperm was measured for the following morphometric parameters: head length, head width, head area, head perimeter, tail length, and the total sperm length. Results: Sperms measured on slides stained with eosin-nigrosin showed lower dimensions as compared with those stained with the eosin-gentian dye method. Sperm stained with eosin-nigrosin had shorter and narrower heads than sperm stained with eosin-gentian dye. The method of staining, therefore, affected not only the dimensions of the sperm, but also the proportions of the dimensions defining the shape of the sperm. Conclusions: The size and shape parameters in porcine sperm may take on different values depending on the method of semen staining. Sperm cells stained with eosin-nigrosin are smaller than the sperm stained with eosin-gentian dye. The sensitivity of the sperm to the type of dye used for the fixation may be associated with genetic factors.
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Al-Sadoon, Ali. "Effect of Aqueous extract of Moringa Olivera leaves( AEMOL) on some characteristics of ram epididymal sperms under cooling conditions." Muthanna Journal for Agricultural Sciences 10, no. 2 (December 15, 2023): 1–6. http://dx.doi.org/10.52113/mjas04/10.2/12.
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The current study was conducted in the Graduate Studies Laboratory at the College of Agriculture, Al-Muthanna University, for the period from December 3, 2022 to March 9, 2023, to determine the effect of an aqueous extract of Moringa leaves on some characteristics of epididymal sperm of rams under cooling conditions for different periods. The aqueous extract of Moringa leaves was prepared and stored in the refrigerator ( 5 °C) until the time of use, the testicles were transferred to the laboratory less than an hour after the slaughter of the animal, the caudal epididymis was diluted with a Tris extender containing 0.03 concentration of aqueous extract of moringa olivera leaves.The results of this study showed a significant increase (P<0.01) in the percentage of live sperm, individual motility, and the plasma membrane integrity, while the adding of AEMOL leads to a decreasing of the percentage of abnormal sperms. Increasing the cooling hours of sperm leads to a significant decrease in the percentage of live sperm, individual motility, testing the integrity of the plasma membrane with an increase in the percentage of abnormal sperm. Using the aqueous extract of Moringa leaves at different times led to a significant effect on the individual motility, percentage of the plasma membrane integrity represented by the hypo-osmotic swelling test (HOST) , While the treatment did not significantly affect the percentage of live sperm and abnormal sperm. According to the results of this study we conclude that the aqueous extract of Moringa Oleifera leaves improves the characteristics of rams epididymal sperms preserved under cooling conditions for different periods
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Idris, Rosila, and Hadi Hartamto. "Logam Berat, Radiasi, Diet, Rokok, Alkohol, dan Obat-obatan Sebagai Penyebab Infertilitas Pria." Jurnal Keperawatan Indonesia 10, no. 2 (April 24, 2014): 70–75. http://dx.doi.org/10.7454/jki.v10i2.176.
Full textAbstract:
AbstrakInfertilitas pada pria dapat disebabkan berbagai faktor seperti logam berat, radiasi, diet, rokok, alkohol, dan obat-obatan. Logam berat yang sering dihubungkan sebagai penyebab gangguan proses spermatogenesis adalah timbal dan kadmium. Radiasi pada intensitas tinggi dapat menyebabkan kerusakan sperma atau mutasi. Konsumsi yang mengandung kortison dan leptin dalam jumlah banyak dapat mempengaruhi produksi FSH dan LH yang mengakibatkan kuantitas sperma berkurang. Asap rokok dapat mengakibatkan penurunan kuantitas atau kualitas sperma pada perokok aktif maupun pasif. Sedangkan alkohol dan obat-obatan dapat menurunkan fertilitas pria melalui jalur gangguan sistem hormon reproduksi. Gangguan hormonal dapat menyebabkan gangguan spermatogenesis, sehingga terjadi gangguan kualitas atau kuantitas sperma. AbstractInfertility on male can be cause by some factors such as heavy metal, radiation, diet, cigarette, alcohol, and drugs. Heavy metals that often related to influence the process of spermatogenesis are plumbum and cadmium. Radiation on the high intensity can destroy sperm or mutation. Consumption of cortisone and leptin in the large number will influence the production of FSH and LH that will decrease the quantity of sperm. Cigarette smoker can decrease the quantity or sperm quality on active and passive smoker. While alcohol and drugs can decrease the fertility of male through interference on reproductive hormone system. Hormonal interferences can cause/effect on spermatogenesis, so that caused decreased quality or quantity of sperm.
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Dupré, E., and G. Schatten. "Actin filaments in two spermatozoa of crustacea decapoda." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (August 12, 1990): 70–71. http://dx.doi.org/10.1017/s0424820100157899.
Full textAbstract:
Sperm of decapod crustaceans are formed by a round or cup-shaped body, a complex acrosome and one a few appendages emerging from the main body. Although this sperm does not have motility, it has some components of the cytoskeleton like microtubules, which are found inside the appendages. Actin filaments have been found in the spike of penaeidae sperms. The actual participation of the crustacean decapod sperm cytoskeleton during fertilization is not well understood. Actin is supposed to play an active role in drawing the penaeidae shrimp sperm closer to the egg after bending of the spike. The present study was aimed at the localization of actin filaments in sperm of the Robinson Crusoe island lobster, Jasus frontalis and in the crayfish Orconectes propincus, by fluorescent probes and low voltage scanning electron microscopy.