Dissertations / Theses on the topic 'Sperm membrane'
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Huang, Wenxin, and 黃聞馨. "Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/196485.
Full textpublished_or_final_version
Obstetrics and Gynaecology
Doctoral
Doctor of Philosophy
Sartini, Becky Lynn. "Characterization of boar sperm plasma membrane candidate oocyte membrane fusion proteins and investigation of oocyte membrane binding sites in boar sperm populations /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2002. http://uclibs.org/PID/11984.
Full textDetmar, Jacqueline. "Studies on putative sex chromosome-specific antigens of bovine sperm membrane." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0016/MQ47320.pdf.
Full textRichmond, Alissa Gale. "Sperm-Oocyte Membrane Interactions during Fertilization in the Nematode Caenorhabditis elegans." W&M ScholarWorks, 2004. https://scholarworks.wm.edu/etd/1539624377.
Full textFortuin, Kay Arlene. "Analyses of spermatozoa surface proteins using different separation techniques." Thesis, University of the Western Cape, 2013. http://hdl.handle.net/11394/4607.
Full textPassage of spermatozoa through the female reproductive tract is essential for the regulation of fertilization, ensuring that healthy sperm reach the oocyte. Previous studies were devoted to morphological selection of sperm cells by the cervical mucus. However, research prove that the loss of integrity of the sperm plasma membrane is associated with infertile men, irrespective of their normal semen parameters. This indicates that the sperm plasma membrane plays an important role in fertilization. Further studies indicated that sperm surface proteins assist penetration through the female reproductive tract and would therefore provide useful insight in understanding other factors associated with male infertility. The aim of this project was to determine if there are any differences between sperm surface proteins of fertile donor samples in relation to infertile patient samples using different separation techniques and different detergents. Three different sperm separation techniques were employed, including wash, swim-up (SU) and Percoll density gradient centrifugation (DGC).Parallel to this, the deoxy-ribose nucleic acid (DNA) fragmentation of these cells were analysed for comparison of the extent of DNA damage induced due to different separation techniques used. This provided evidence that the best separation technique is the DGC as it minimises the amount of DNA fragmentation caused. Four different detergents were used in the process of extracting the membrane proteins from spermatozoa, namely sodium dodecyl sulphate (SDS), saponin,cetyl-trimethyl-ammonium bromide (CTAB), and TWEEN-20. The membrane proteins were then separated on a12% SDS poly-acrylamide gel electrophoresis (PAGE), and analysed by Coomassie blue and silver staining techniques as well as densitometry. Due to the different chemical nature of the detergents that extracted different surface proteins, CTAB (cationic) and SDS (anionic) extracted the most because of its strong solubilising abilities as non-ionic detergents. Common proteins that were extracted in donor samples included; 115, 92.5, 89, 61, 55.5, 51.5, 47, 44.5, 43, 38.5, 34 and 28 kDa proteins. In patients, commonly occurring proteins included; 92.5, 74.5, 70, 60.5, 51.5, 50, 44.5, 43, 36, 29.5, and 25.5 kDa proteins. Marked differences were found between membrane proteins extracted from donor samples in comparison to patient samples. Identification of these proteins was done using the SwissProt database and a literature search. Mostly non-genomic progesterone receptors were identified; others included oestrogen receptor, a phosphotyrosyl protein, P34H, equatorial segment protein, mannose lectin receptor, human guanylylcyclase receptor, epididymal protease inhibitor receptor, PH30 and estradiol binding protein. The function of the membrane surface proteins identified in this study plays a vital role in fertilization. A few of these functions include sperm attachment and binding to the oocyte as well as penetration thereof. Others play a role in signalling events such as capacitation, hyperactivation and acrosome reaction. The absence of these proteins in patient sperm possibly accounts for the functional inability to successfully achieve fertilization suggesting that this provides molecular insight to reasons for infertility amongst men. In addition to this, proteins presented by patient samples that were absent in healthy donors may too account for their infertility status. Estradiol binding protein and PH30 are two proteins presented only in patient samples. Their function plays a role in the inhibition of the acrosome reaction and sperm-egg fusion, respectively. In conclusion, these differences in protein expression between fertile donors and patients may form the molecular basis of infertility amongst men and indicates possibilities for novel proteonomic approaches to improve andrological diagnosis in future.
Vicente, Carrillo Alejandro. "Sperm Membrane Channels, Receptors and Kinematics : Using boar spermatozoa for drug toxicity screening." Doctoral thesis, Linköpings universitet, Avdelningen för kliniska vetenskaper, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-131862.
Full textSlabbert, Marisa. "Investigating alternative sperm preservation methods for assisted reproductive technologies." Diss., University of Pretoria, 2013. http://hdl.handle.net/2263/40838.
Full textDissertation (MSc)--University of Pretoria, 2013.
gm2014
Obstetrics and Gynaecology
unrestricted
Fußhöller, David [Verfasser]. "The voltage‐gated H+ channel Hv1 and the plasma‐membrane Ca2+ ATPase PMCA4 in human sperm / David Fußhöller." Bonn : Universitäts- und Landesbibliothek Bonn, 2015. http://d-nb.info/1113688157/34.
Full textYuen, Chung-yat. "Effects of secretions from ampullary gland and ventral prostate on the sperm plasma membrane of golden hamster (mesocricetus auratus) /." [Hong Kong] : University of Hong Kong, 1993. http://sunzi.lib.hku.hk/hkuto/record.jsp?B13447488.
Full textBlässe, Anne-Kathrin [Verfasser]. "Effects of cryopreservation on osmotic membrane properties, intracellular ion distribution, and ion channels of bovine sperm / Anne-Kathrin Blässe." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2012. http://d-nb.info/1024339556/34.
Full textFrancisco, Júnior Arthur. "Qualidade do sêmen equino criopreservado com L-acetil-cisteina." Universidade Federal de Goiás, 2014. http://repositorio.bc.ufg.br/tede/handle/tede/4034.
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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
This study was conducted to evaluate the effect of adding L-acetyl-cysteine (CIS) as anti oxidant agent to extender used for sperm cryopreservation of equine Mangalarga Marchador. Three semen samples from seven healthy stallions were obtained by artificial vagina, diluted in commercial extender and distributed into two treatments: control without addition and CIS, containing the antioxidant concentration of 2.5 mM. After the stabilization period, the samples were filled into 0.5 mL straws and subjected to cryopreservation protocol manually. Sixty days after the samples were thawed and evaluated in vitro by means of computerized analysis of sperm kinetics (CASA) and the evaluation of the integrity of plasma and acrosomal membranes by fluorescent probes. Regarding the kinetic variables related to sperm was found that in samples supplemented with CIS percentage of total and progressive motility (TM) (MP) was higher (P <0.01) compared to control samples (23.95±3 , 33 X 11 .38±2.35 and 4.66 ± 0.84 X 2.04 ± 0.36, respectively), but variable for straightness (STR) and linearity (LIN) the percentages were highe r (p ≤ 0.01) for the control samples (86.95 ± 1.02 X 82.28 ± 1.02 and 49.85 ± 1.32 X 45.19 ± 1.17, respectively). As those related to the integrity of the membranes of the sperm variables was superior (P <0.05) in the number of sperm with intact acrosome reaction (ICRA) in the samples compared to those CIS Control (4.7 ± 1.6 x 0.8 ± 0.2, respectively). The results suggest that the addition of acetyl-L-cysteine concentration of 2.5 mM in the diluent medium before cryopreservation can improve both the motility as the integrity of the sperm plasma membrane equine Mangalarga Marcher. More studies should be done to confirm this effect in vivo.
Esse estudo foi conduzido com o objetivo de avaliar o efeito da adição de L-acetilcisteína (CIS) como agente anti oxidante ao meio diluente utilizado para criopreservação de espermatozóides de equinos da raça Mangalarga Marchador. Três amostras seminais de sete garanhões hígidos foram obtidas por meio de vagina artificial, diluídas em meio comercial e distribuídas em dois tratamentos: controle, sem adição e CIS, contendo o antioxidante na concentração de 2,5 mM. Após o período de estabilização as amostras foram envasadas em palhetas de 0,5 mL e submetidas ao protocolo de criopreservação manual. Sessenta dias após as amostras foram descongeladas e avaliadas in vitro por meio da análise computadorizada da cinética espermática (CASA) e da avaliação da inte gridade das membranas plasmática e acrosomal por meio de sondas fluorescentes. Em relação às variáveis relacionadas à cinética espermática verificou-se que nas amostras suplementadas com CIS o percentual de motilidade total (MT) e progressiva (MP) foi superior (P<0,01) em relação às amostras controle (23,95±3,33 X 11,38±2,35 e 4,66±0,84 X 2,04±0,36, respectivamente), mas para as variáveis retilinearidade (STR) e linearidade (LIN) os percentuais foram maiores (P≤0,01) para as amostras Controle (86,95±1,02 X 82,28±1,02 e 49,85±1,32 X 45,19±1,17, respectivamente). Quanto às variáveis relacionadas à integridade das membranas do espermatozóide houve superioridade (P<0,05) no número de espermatozóides íntegros com reação acrossomal (ICRA) nas amostras CIS em relação àquelas controle (4,7±1,6 X 0,8±0,2, respectivamente). Os resultados sugerem que a adição de L-acetil-cisteína na concentração de 2,5 mM ao meio diluente antes da criopreservação pode melhorar tanto a motilidade quanto a integridade da membrana plasmática de espermatozóides de equinos da raça Mangalarga Marchador. Mais estudos devem ser feitos para comprovar esse efeito in vivo.
Kongmanas, Kessiri. "Roles of Seminolipid and Its Associated Membrane Domain in Male Fertility." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32509.
Full textMuhlrad, Paul, Jessica Clark, Ubaydah Nasri, Nicholas Sullivan, and Craig LaMunyon. "SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegans." BioMed Central, 2014. http://hdl.handle.net/10150/610393.
Full textGojowsky, Marina [Verfasser]. "Fourier transform infrared spectroscopy studies on freezing-induced membrane phase behavior of stallion sperm in the presence of cryoprotective agents / Marina Gojowsky." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2012. http://d-nb.info/1024421678/34.
Full textFonseca, Lucas dos Santos. "Performance, dry matter intake, seminal parameters and proteomics of seminal plasma and sperm membrane of Morada Nova sheep fed the diet cashew nut base." Universidade Federal do CearÃ, 2013. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11466.
Full textO presente estudo foi conduzido com o objetivo de avaliar os efeitos da inclusÃo de 13% de farelo de castanha de caju na dieta de carneiros da raÃa Morada Nova sobre o ganho de peso, consumo de matÃria seca, rendimento de carcaÃa, pesos dos ÃrgÃos sexuais, qualidade seminal e proteÃnas seminais e membranares do espermatozoide. Para tanto, vinte carneiros foram divididos em dois grupos alimentados com dietas contendo 13% (GCA, n=10) ou 0% (GCO, n=10) de farelo de castanha de caju. As dietas foram isoproteicas e isoenergeticas, com uma relaÃÃo de 50/50 de concentrado/volumoso (feno de Tifton 85) e Ãgua e sal mineral à vontade. Durante noventa dias, os animais foram mantidos em baias individuais e avaliados quanto ao consumo de matÃria seca e ganho de peso. As coletas seminais por eletroejaculaÃÃo ocorreram semanalmente com a determinaÃÃo do volume ejaculado e, concentraÃÃo, motilidade e morfologia espermÃtica. ApÃs os noventa dias, as proteÃnas seminais e espermÃticas foram analisadas por meio de eletroforese bidimensional em gel de poliacrilamida. Os mapas proteÃcos foram avaliados por meio do aplicativo PDQuest, version 8.0; Bio Rad, USA. Ao final do experimento, os carneiros foram abatidos e, o peso vivo, rendimento de carcaÃa e peso dos ÃrgÃos sexuais foram mensurados. O consumo de matÃria seca total permaneceu sem alteraÃÃes nos dois grupos experimentais durante os primeiros 60 dias do estudo. PorÃm, apÃs 60 dias, houve e reduÃÃo no consumo de matÃria seca no grupo alimentado com castanha (P < 0,05). NÃo houve efeito da inclusÃo do farelo de castanha sobre o ganho de peso, rendimento de carcaÃa, desenvolvimento dos ÃrgÃos sexuais e parÃmetros seminais dos animais. No entanto, a inclusÃo do farelo de castanha de caju esteve associada à expressÃo de proteÃnas seminais e espermÃticas. Sete spots proteicos presentes nos mapas de plasma seminal foram expressos diferencialmente entre os grupos castanha e controle (P < 0,05) e um spot proteico (21,8 kDa; pI: 5) correlacionou-se negativamente com o vigor espermÃtico (r = -0,49; P<0,05). Adicionalmente, sete spots proteicos dos gÃis com proteinas da membrana dos espermatozoides tambÃm diferiram entre os carneiros alimentados com e sem farelo de castanha de caju (P < 0,05). Conclui-se, portanto, que a inclusÃo de 13% de farelo de castanha de caju na dieta altera a expressÃo de proteÃnas seminais e espermÃticas em ovinos.
The cashew nut meal is alternative food for ruminant nutrition which is rich in lipids, proteins and minerals. However, its nutrition influences on male development and reproduction are still unknown. In this context, seminal and sperm protein analysis can show metabolic consequences from cashew nut meal on sheep reproductive efficiency. Therefore, the current dissertation aims evaluate the effects of 13% of cashew nut meal in the ration on the weight gain, food intake, carcass yield, weight of sexual organs, sperm quality and, seminal and sperm membrane proteins of Morada Nova Rams. Twenty rams were divided in two groups: cashew nut and control, which received 13% or 0% of cashew nut meal in the diet, respectively. During ninety days, the animals were kept in individual boxes and evaluated for ration consumption and weight gain. The sperm collections by eletroejaculation were made by weekly and, the volume, sperm concentration and motility were analyzed. After ninety days, the seminal and sperm membrane proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. The gels were digitalized, and their images were evaluated by computer software. At the end of the experiment, the rams were slaughtered and, the weight gain, carcass yield and weight of sexual organs were measured. There was not effect of cashew nut meal addition in the diet on the weight gain, carcass yield and weight of sexual organs. But, after sixty days, there was a reduction on food intake in the cashew nut group (P < 0,05). The sperm quality was not influenced by the diet. We observed an effect of cashew nut diet on the expression of seminal and sperm proteins. Seven spots from seminal plasma were expressed differently between cashew nut and control groups (P < 0,05). The spot 2206 (21,82 kDa; pI: 5,04) was negatively correlated with the individual motility score (r = -0,49). Additionally, seven spots from sperm membrane protein also differ between rams from cashew nut and control groups (P < 0,05). In conclusion, the addition of 13% of cashew nut meal in the diet alters the expression of sheep seminal plasma and sperm membrane proteins.
Kaydos, Emilie Hayes. "Fertility and the sperm membrane biomarker (SP22) are compromised in an additive fashion by priority disinfection by-products of drinking water a validation of enzyme linked immunosorbant assay (ELISA) for SP22 /." NCSU, 2004. http://www.lib.ncsu.edu/theses/available/etd-04132004-133325/.
Full textKurz, Anke. "Organisation und Dynamik der Phospholipide in der Zell- und Akrosommembran von Eberspermien während der Kapazitation und Akrosomreaktion." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät II, 2005. http://dx.doi.org/10.18452/15274.
Full textOne of the essential qualities of cell membranes in Eucaryotae is a stable transverse phospholipid asymmetry. It is regulated and maintained by ATP-dependent action of an aminophospholipid translocase and is a major prerequisite for cell homeostasis. The plasma membranes of several mammalian cells show moreover lateral lipid domains, which are imputed to play a significant role in signal transduction. The membranes of mammalian spermatozoa undergo significant changes during genesis. The localisation and dynamics of phosphatidylserine in the cell as well as acrosome membranes of boar sperm cells was studied during capacitation and acrosome reaction to assess the relevance of lipid asymmetry for sperm function. The localisation of endogenous phosphatidylserine in morphologically differentiated cells was followed using the selective calcium depending binding of annexinV. Information on the transverse dynamics of phospholipids in the plasma membrane was obtained by labelling the cells with a NBD-phospholipid analogues. The morphological status of the cells was assessed by flow cytometry, fluorescence and electron microscopy. The results of this study indicate both a transversal and lateral inhomogenous distribution of lipids in the cell membrane as well as in the outer acrosome membrane during sperm genesis. The plasma membrane of boar sperm shows a stable transversal lipid asymmetry characterised by an accumulation of phosphatidylserine in the cytoplasmic monolayer. Moreover a cytoplasmic localisation of phosphatidylserine on the outer acrosome membrane could be detected for the first time. Therefore the two facing cytoplasmic leaflets of the outer acrosome and cell membrane contain phosphatidylserine. Applying microscopy substantiated the hypothesis that there are close interactions between the cell membrane and the outer layer of the acrosome membrane because of capacitation. The cytoplasmic accumulation of phosphatidylserine in lateral lipid domains is probably essential for the strong association of plasma and outer acrosome membrane finally leading to local fusions of both membranes. An indication for the functional meaning of lateral membrane domains in sperm cells was futher deduced from the isolation of Triton-insoluble lipid domains from membranes of trout sperm cells.
Springsguth, Hans Christopher. "Mechanismen und Bedeutung der aktivierten Apoptosekaskade in humanen Spermatozoen." Doctoral thesis, Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-192660.
Full textKavak, Ants. "Evaluation of sperm production, testicular measurements and post-thaw sperm quality in Tori and Estonian breed stallions /." Uppsala : Dept. of Obstetrics and Gynaecology, Swedish Univ. of Agricultural Sciences, 2004. http://epsilon.slu.se/9329559.pdf.
Full textFierro-Pastrana, Reyna-Carmen. "Etude de l'architecture moléculaire de la membrane plasmique et des membranes acrosomiques du spermatozoïde humain : leurs modifications au cours des phénomènes de capacitation et de la réaction acrosomique." Nancy 1, 1997. https://hal.univ-lorraine.fr/tel-01747360.
Full textBrandão, Alessandra Cunha. "Efeito do laser diodo sobre as características de motilidade, de integridade das membranas plasmática e acrossomal e de potencial de membrana mitocondial de espermatozóides criopreservados de eqüinos." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-09012009-162214/.
Full textSperm motility depends on energy consumption. In some species sperm mitochondria play an important role in the production of energy for tail activity. Low-level laser irradiation increases this production as a modulation tool. The objective of this study was to analyze the effect of a continuous 650 nm wavelength diode laser irradiation, with dose 6 J/cm2 for 120s, in the motility, plasma and acrosomal membrane integrity and mitochondrial membrane potential in fresh and frozen equine spermatozoa. Five ejaculates were obtained from five stallions (n=25). Semen was packaged into 0.5mL straws with 200x106 cells/mL in a Botu-CrioTM (Biotech-Botucatu-Ltda/ME, Botucatu, Brazil) and frozen by automated technique using a programmed machine (TK3000®, TK Tecnologia em Congelação-Ltda, Uberaba, Brazil). Fresh samples were divided in two groups: spermatozoa treated with laser and without laser (non treated spermatozoa), and frozen samples in three groups: spermatozoa treated with laser before freezing; spermatozoa treated with laser after thawing and without laser (cryopreserved spermatozoa). Cryopreserved samples were analyzed immediately after thawing (time 0) and two hours after thawing (time 2). Motility was evaluated by computer assisted sperm analysis (CASA, Ivos-Ultimate of Hamilton Thorne Biosciences), plasma and acrosomal membrane integrity and mitochondrial membrane potential were evaluated by flow cytometry (FACSaria-Beckton-Dickeson, San Jose, USA). The data were analyzed by the SAS program, at a 5% level. Beat cross frequency (BCF) test was higher (p<0.05) for fresh semen group treated with laser (34.8 ± 0.7%) as compared to non treated group (without Laser - 33.4 ± 0.8%). At time zero, mitochondrial membrane potential was lower in laser treatment before freezing group (40.7 ± 1.5%); compared to without laser treatment group (cryopreserved spermatozoa - 47.4 ± 2.4%) (P<0.05). Two hours after thawing, plasma and acrosomal integrity was higher (P<0.05) in the group were spermatozoa were treated with laser before freezing (8.3 ± 0.7%) compared with the group without laser treatment (cryopreserved spermatozoa) (6.2 ± 0.6%). The group treated with laser after thawing didn´t show any difference (P>0.05) compared to the others groups at this period. However, at time 0 percentage of progressive motility was lower (2.0 ± 0.3%) (P<0.05) than groups treated with laser before freezing (6.5 ± 1.3%) and cryopreserved without laser (5.5 ± 1.1%). These results indicated that the irradiation of 650 nm wavelength diode laser improves beat cross frequency in the fresh semen and support a long term (2 hours) protection to plasma and acrosomal membrane of equine spermatozoa. Based on the results of frozen semen, the best moment for laser application is before cryopreservation protocol. New studies with different diode laser wavelength, different power and energy doses should be driven in order to improve stallion semen cryopreservation.
Schröter, Filip [Verfasser]. "Recombinantly expressed spermadhesin AWN and fluorescence based characterization of porcine sperm membranes - prerequisites for functional interaction studies / Filip Schröter." Berlin : Freie Universität Berlin, 2017. http://d-nb.info/1136608710/34.
Full textBotha, Alma Ester. "Effect of the acidic buffer 2-(N-Morpholino) ethanesulfonic acid on frozen-thawed bull semen." Diss., University of Pretoria, 2009. http://hdl.handle.net/2263/22848.
Full textDissertation (MSc (Veterinary Science))--University of Pretoria, 2008.
Production Animal Studies
unrestricted
Bou, Khalil Maroun. "Biophysical and biochemical properties of the mammalian male germ cell specific sulfogalactosylglycerolipid (SGG): Contribution to the structure and zona pellucida affinity of pig sperm raft membranes." Thesis, University of Ottawa (Canada), 2004. http://hdl.handle.net/10393/29079.
Full textRodríguez, Shirley Andrea Flórez. "Efeitos de diferentes diluidores sobre a cinética, membranas, morfologia e cromatina espermáticas durante a refrigeração de sêmen equino." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-06052013-092347/.
Full textTo develop the efficiency of cooling semen is the great advantage in the equine reproduction, as according to the equine population to grow up, too increase the demand to market and transport of cooling semen. This experiment was performed to verify the effects of different extenders to equine cooling semen at 5°C on the sperm kinetic, membranes, morphology and chromatin during 12 hours of storage. Were utilized four ejaculates from four stallions of different breeds, collected a week. Immediately after the collection, the in natura semen was evaluated as the sperm movement using the Computer-Assisted Semen Analysis (CASA), integrity of plasma and acrossomal membranes and mitochondrial membrane potential, using the fluorescent probes (PI, H342, FITC-PSA and JC-1, by epifluorescência microscopy), sperm morphology by microscopy of differential interference contrast (DIC) and chromatin denaturation by Toluidine blue. As soon as finished the analyses, the semen was diluted using three different extenders: SMT, skim milk based and Tyrode medium (adapted from PADILLA; FOOTE, 1991), BSAG, extender containing BSA (adapted from GIBB et al., 2011) and SMK, skim milk based (KENNEY et al., 1975; control), following was packed in flasks at 50 x 106sperm/mL concentration and cooling at 5°C in BotuFLEX® (Botupharma, Botucatu-SP) box, during 12 hours. The semen was analyzed 5 minutes after dilution (T0), 4 (T4), 8 (T8) and 12 hours (T12) after cooling about sperm movement, plasma and acrossomal membranes integrity and mitochondrial membrane potential sperm morphology and chromatin denaturation. The statistical analysis was performed using Statistical Analysis System (SAS inst. Inc.) software. To all variables was utilized the analysis of variance (ANOVA). To media comparison was utilized the Tukey method, considering the significant level at 5%. Were observed interactions between times X treatment to majority of sperm kinetic characteristic. Among the found effects of the extenders, it was detached that SMT and SMK were superior to preserve total and progressive motility and percentage of rapid sperm in detriment to BSAG extender. It about the membranes preservation the SMT extender (56.6±18.7%) was significantly superior to SMK (49.6±18.6%), which was superior to BSAG (25.8±14.8%) as percentage of sperm with plasma and acrossomal membranes integrity and high mitochondrial potential (PIAIA). Behavior similar was observed to the plasma membrane integrity and potential mitochondrial membrane percentage. Contrary, The acrossomal membrane was not affected by semen cooling at 5ºC until 12 hours independently of the extender. It was found major percentage of defect total to cooling semen using BSAG (50±12.4%) extender compared to SMT (42.6±11.2%) and SMK (41.8±12.4%). When evaluated the sperm chromatin were not found effect of the cooling time neither of the extenders; however, when compared to in natura semen notice an increase on the percentage of spermatozoa with moderate chromatin denaturation after 12 hours of cooling at 5ºC with BSAG extender. It was concluded that the equine semen cooling at 5ºC change the sperm kinetic oscillating way among extenders during the time until 12 hours. The SMT and SMK extenders are better to preserve the sperm kinetic, membranes integrity and morphology than BSAG extender.
Gallego, Andrés Mejia. "Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-01042011-143106/.
Full textSex selecting has a huge impact in dairy and beef production. The only way commercially viable to obtain sex selecting nowadays, is a flow citometry sex sorted sperm technique. Currently sex sorted sperm have been used regularly in Artificial Insemination programs. Meanwhile, there are limitations about non return rate in farm conditions, superficially explained as cellular loss integrity. The objective of this experiment was assessing sex sorted sperm effects and time to wait 0, 3 and 6 hours before sorting in motility parameters by computer assisted sperm analysis (CASA), plasmatic membrane functionality by hiposmotic swelling test (HOST), sperm morphology by differential interference contrast microcopy (DIC) and between taurine subspecies (Bos taurus taurus) zebuine subspecies (Bos taurus indicus). Sex sorted sperm showed best results in the most motility parameters compared with conventional sperm, suggesting a flow citometer selection. The time to wait of the sperm (untis 6 hours) to keep sorting, altered a linearity (LIN). Plasmatic membrane functionality was affected when sperm runs through flow citometer.. Sex sorted induces increase of minor defects in sperm, probably by tail membrane plasmatic alterations. Zebuin sperm are more sensitive for tail plasmatic membrane alterations compared with taurine sperm. Finally, there is strong indication that bovine sperm that runs through flow citometer sign in hiperactivation state.
Azevedo, Hymerson Costa [UNESP]. "Integridade e funcionalidade dos espermatozóides ovinos submetidos à criopreservação após a incorporação de colesterol, desmosterol, ácido oléico-linoléico e alfa-lactoalbumina." Universidade Estadual Paulista (UNESP), 2006. http://hdl.handle.net/11449/105970.
Full textEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Objetivando testar a ação do colesterol, desmosterol, ácido oléico-linoléico ou Q-Iactoalbumina sobre os espermatozóides ovinos, alíquotas de sêmen de 25 carneiros foram submetidas à criopreservação após tratamento com essas substâncias. O sêmen foi avaliado antes do processamento e após a refrigeração, descongelação e incubação quanto à, cinética pela análise subjetiva e computadorizada (CASA), morfologia, avaliação simultânea da integridade da membrana plasmática (IMP) e do acrossomo (IAC) e do potencial de membrana mitocondrial (PMM) pela associação da aglutinina de Pisum sativum conjugada ao isotiocianato de fluoresceína (FITC-PSA), iodeto de propídio (PI) e JC-1 e, status de capacitação pela c10rtetraciclina (CTC). A influência dos aditivos em poucos parâmetros não foi suficiente para sugerir a superioridade de qualquer um dos tratamentos. A criopreservação provocou prejuízos à cinética, IMP, IAC, PMM, além de induzir a capacitação e reação do acrossomo. Foram observadas diferenças entre grupos de carneiros quanto ao status de capacitação no sêmen in natura, assim como na sensibilidade desse sêmen à crioinjúria e criocapacitação. Após a descongelação, as mudanças semelhantes à capacitação foram maiores nos animais de menor IMP. A segregação dos carneiros de acordo com a crioresistência da membrana plasmática norteou o comportamento de vários outros parâmetros. Conclui-se, que o colesterol, desmosterol, ácido oléico-linoléico e alactoalbumina não protegem o sêmen ovino contra as crioinjúrias, que a congelação-descongelação é mais danosa que a refrigeração e, que existem diferenças entre grupos de indivíduos relacionadas à crioresistência e criocapacitação espermática.
In order to evaluate the proteeting aetion of some substanceson ram spermatozoa, semen samples of 25 rams were colleeted and submitted to cryopreservation after treatment with cholesterol, desmosterol, oleic-linoleic acid or Q-Iactalbumin. Before the processing and after the procedures of cooling, freezing-thawing and incubation, semen was evaluated as to kínetcs by subjective and computerized analyses (CASA), morphology, simultaneously to plasma membrane integrity (PMI), acrosome integrity (ACI) and mitochondrial membrane potential (MMP) by isothiocyanate-conjugated Pisum sativum (FITCPSA), propidium iodide (PI) and JC-1 combination and as to sperm capacitation and acrosome reaction determined by chlortetracycline (CTC) assay. The influence resulted from the additives in a few parameters was not enough to suggest the superiority of any treatments. The cryopreservation process, especially the freezing-thawing, promotes damages to kinetics, PMI, ACI, MMP and induees accelerated eapacitation and acrosome reaction in spermatozoa. Differenees among groups of rams were observed regarding capacitation status in fresh semen as well as the sensibility of their semen to cryoinjury and cryocapacitation. Higher and more accelerated capacitation-like ehanges were observed in groups of rams presenting lower plasmatic membrane cryoresistance after thawing. The segregation of rams in different levels of PMI influenced the behavior of several other parameters. It was concluded that cholesterol, desmosterol, oleic-Iinoleic acid and Q-Iactalbumin do not protect the ram semen against the cryoinjuries as well as freezing-thawing is more harmful than cooling. Important differenees among groups of individuais were noticed as for the spermatozoa resistance to the damages provoked by cryopreservation such as the eryocapacitation susceptibility.
Labbé, Catherine. "Membrane plasmique du spermatozoide de salmonides : caracterisation biochimique et biophysique : etude des modifications induites par l'alimentation et la temperature d'elevage : relation avec l'aptitude a la congelation du sperme." Rennes 1, 1992. http://www.theses.fr/1992REN10075.
Full textAzevedo, Hymerson Costa. "Integridade e funcionalidade dos espermatozóides ovinos submetidos à criopreservação após a incorporação de colesterol, desmosterol, ácido oléico-linoléico e alfa-lactoalbumina /." Botucatu : [s.n.], 2006. http://hdl.handle.net/11449/105970.
Full textBanca: Rui Machado
Banca: Jairo Pereira Neves
Banca: César Roberto Esper
Banca: Cezinande de Meira
Resumo: Objetivando testar a ação do colesterol, desmosterol, ácido oléico-linoléico ou Q-Iactoalbumina sobre os espermatozóides ovinos, alíquotas de sêmen de 25 carneiros foram submetidas à criopreservação após tratamento com essas substâncias. O sêmen foi avaliado antes do processamento e após a refrigeração, descongelação e incubação quanto à, cinética pela análise subjetiva e computadorizada (CASA), morfologia, avaliação simultânea da integridade da membrana plasmática (IMP) e do acrossomo (IAC) e do potencial de membrana mitocondrial (PMM) pela associação da aglutinina de Pisum sativum conjugada ao isotiocianato de fluoresceína (FITC-PSA), iodeto de propídio (PI) e JC-1 e, status de capacitação pela c10rtetraciclina (CTC). A influência dos aditivos em poucos parâmetros não foi suficiente para sugerir a superioridade de qualquer um dos tratamentos. A criopreservação provocou prejuízos à cinética, IMP, IAC, PMM, além de induzir a capacitação e reação do acrossomo. Foram observadas diferenças entre grupos de carneiros quanto ao status de capacitação no sêmen in natura, assim como na sensibilidade desse sêmen à crioinjúria e criocapacitação. Após a descongelação, as mudanças semelhantes à capacitação foram maiores nos animais de menor IMP. A segregação dos carneiros de acordo com a crioresistência da membrana plasmática norteou o comportamento de vários outros parâmetros. Conclui-se, que o colesterol, desmosterol, ácido oléico-linoléico e alactoalbumina não protegem o sêmen ovino contra as crioinjúrias, que a congelação-descongelação é mais danosa que a refrigeração e, que existem diferenças entre grupos de indivíduos relacionadas à crioresistência e criocapacitação espermática.
Abstract: In order to evaluate the proteeting aetion of some substanceson ram spermatozoa, semen samples of 25 rams were colleeted and submitted to cryopreservation after treatment with cholesterol, desmosterol, oleic-linoleic acid or Q-Iactalbumin. Before the processing and after the procedures of cooling, freezing-thawing and incubation, semen was evaluated as to kínetcs by subjective and computerized analyses (CASA), morphology, simultaneously to plasma membrane integrity (PMI), acrosome integrity (ACI) and mitochondrial membrane potential (MMP) by isothiocyanate-conjugated Pisum sativum (FITCPSA), propidium iodide (PI) and JC-1 combination and as to sperm capacitation and acrosome reaction determined by chlortetracycline (CTC) assay. The influence resulted from the additives in a few parameters was not enough to suggest the superiority of any treatments. The cryopreservation process, especially the freezing-thawing, promotes damages to kinetics, PMI, ACI, MMP and induees accelerated eapacitation and acrosome reaction in spermatozoa. Differenees among groups of rams were observed regarding capacitation status in fresh semen as well as the sensibility of their semen to cryoinjury and cryocapacitation. Higher and more accelerated capacitation-like ehanges were observed in groups of rams presenting lower plasmatic membrane cryoresistance after thawing. The segregation of rams in different levels of PMI influenced the behavior of several other parameters. It was concluded that cholesterol, desmosterol, oleic-Iinoleic acid and Q-Iactalbumin do not protect the ram semen against the cryoinjuries as well as freezing-thawing is more harmful than cooling. Important differenees among groups of individuais were noticed as for the spermatozoa resistance to the damages provoked by cryopreservation such as the eryocapacitation susceptibility.
Doutor
Losano, João Diego de Agostini. "Efeito do tratamento antioxidante sistêmico em touros Bos taurus taurus submetidos ao estresse térmico e suplementados com dieta rica em ácidos graxos poliinsaturados sobre a capacidade de ligação de espermatozóides à membrana vitelínica de ovos de galinha." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-09102013-101625/.
Full textSeveral studies suggest a high susceptibility of European bulls (Bos taurus) to the heat stress, which will lead to an increase on oxidative stress and consequent impairment on sperm viability. Sperm membranes are rich in poly-unsaturated fatty acids (PUFAs). The carbon-carbon double bounds (insaturations) that characterize these PUFAs confer the necessary fluidity to the sperm membrane, essential to motility and fecundation capacity. However, these insaturations are more unstable and, therefore, more susceptible to the lipid peroxidation (i.e., attack of reactive oxygen species to the lipids). Hence, PUFA supplementation would improve certain sperm characteristics but increase the already high susceptibility to the oxidative stress. Vitamin E is a key antioxidante on oxidative stress prevention by intercepting hydroxyl radical and protecting sperm membrane PUFAS against lipid peroxidation. Therefore, an alternative to improve sperm quality in heat stressed european bulls would be a PUFA supplementation associated to a Vitamin E therapy in order to avoid a possible deleterious oxidative effects of these fatty acids. Estudies suggest an important relationship between conventional tests for sperm evaluation and fertility. However, limitations have been also verified, especially in cases of idiopathic infertility. Thus, tests to evaluate sperm functionality may be an interesting alternative to assess fecundation capacity of semen samples. The binding of sperm to the zona pellucida occurs initially by the interaction between acrosomal receptors and oocyte proteins ZP3 and ZP2. The perivitelline membrane of chicken eggs present homologies to zona pellucida proteins, which allows sperm from several species to bind to this membrane. Thus, the sperm binding test to the perivitelline membrane may be an efficient method to asses the fecundation capacity in semen of bulls. In the present study, 16 Bos taurus bulls were submitted to testicular insulation for 4 day. Animals were then submitted to a 2x2 factorial design, with the factor represented by a PUFA oral supplementation (Megalac®, 4 Kg for day) and the treatment with Vitamin E (Monovin E®, 3000 UI, each 13 days) for 60 days. Samples were cryopreserved/thawed and analyzed for motility variables by the computer assisted sperm analysis (CASA; Hamilton Thorne, Ivos 12.3, USA), membrane integrity (Eosin / Nigrosin), acrosome integrity (Fast Green / Bengal Rose), mitochondrial activity (33 diaminobenzidine), susceptibility to the oxidative stress (tiobarbituric acid reactive substances - TBARS), DNA integrity (SCSA) and the sperm binding test to the chicken egg perivitelline membrane, which was previously validated. Results demonstrated a beneficial effect of Vitamin E on some sperm characteristics, with the inverse effect observed for PUFA when both treatments were considered individually. However, the association between both treatments were not efficient to improve sperm quality as well as the fecundation capacity as evaluated by the sperm binding test to the chicken perivitelline membrane.
Fonseca, Lucas dos Santos. "Desempenho, consumo de matéria seca, parâmetros seminais e proteômica do plasma seminal e da membrana espermática de carneiros Morada Nova alimentados com dieta à base de castanha de caju." reponame:Repositório Institucional da UFC, 2013. http://www.repositorio.ufc.br/handle/riufc/14861.
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The cashew nut meal is alternative food for ruminant nutrition which is rich in lipids, proteins and minerals. However, its nutrition influences on male development and reproduction are still unknown. In this context, seminal and sperm protein analysis can show metabolic consequences from cashew nut meal on sheep reproductive efficiency. Therefore, the current dissertation aims evaluate the effects of 13% of cashew nut meal in the ration on the weight gain, food intake, carcass yield, weight of sexual organs, sperm quality and, seminal and sperm membrane proteins of Morada Nova Rams. Twenty rams were divided in two groups: cashew nut and control, which received 13% or 0% of cashew nut meal in the diet, respectively. During ninety days, the animals were kept in individual boxes and evaluated for ration consumption and weight gain. The sperm collections by eletroejaculation were made by weekly and, the volume, sperm concentration and motility were analyzed. After ninety days, the seminal and sperm membrane proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. The gels were digitalized, and their images were evaluated by computer software. At the end of the experiment, the rams were slaughtered and, the weight gain, carcass yield and weight of sexual organs were measured. There was not effect of cashew nut meal addition in the diet on the weight gain, carcass yield and weight of sexual organs. But, after sixty days, there was a reduction on food intake in the cashew nut group (P < 0,05). The sperm quality was not influenced by the diet. We observed an effect of cashew nut diet on the expression of seminal and sperm proteins. Seven spots from seminal plasma were expressed differently between cashew nut and control groups (P < 0,05). The spot 2206 (21,82 kDa; pI: 5,04) was negatively correlated with the individual motility score (r = -0,49). Additionally, seven spots from sperm membrane protein also differ between rams from cashew nut and control groups (P < 0,05). In conclusion, the addition of 13% of cashew nut meal in the diet alters the expression of sheep seminal plasma and sperm membrane proteins.
O presente estudo foi conduzido com o objetivo de avaliar os efeitos da inclusão de 13% de farelo de castanha de caju na dieta de carneiros da raça Morada Nova sobre o ganho de peso, consumo de matéria seca, rendimento de carcaça, pesos dos órgãos sexuais, qualidade seminal e proteínas seminais e membranares do espermatozoide. Para tanto, vinte carneiros foram divididos em dois grupos alimentados com dietas contendo 13% (GCA, n=10) ou 0% (GCO, n=10) de farelo de castanha de caju. As dietas foram isoproteicas e isoenergeticas, com uma relação de 50/50 de concentrado/volumoso (feno de Tifton 85) e água e sal mineral à vontade. Durante noventa dias, os animais foram mantidos em baias individuais e avaliados quanto ao consumo de matéria seca e ganho de peso. As coletas seminais por eletroejaculação ocorreram semanalmente com a determinação do volume ejaculado e, concentração, motilidade e morfologia espermática. Após os noventa dias, as proteínas seminais e espermáticas foram analisadas por meio de eletroforese bidimensional em gel de poliacrilamida. Os mapas proteícos foram avaliados por meio do aplicativo PDQuest, version 8.0; Bio Rad, USA. Ao final do experimento, os carneiros foram abatidos e, o peso vivo, rendimento de carcaça e peso dos órgãos sexuais foram mensurados. O consumo de matéria seca total permaneceu sem alterações nos dois grupos experimentais durante os primeiros 60 dias do estudo. Porém, após 60 dias, houve e redução no consumo de matéria seca no grupo alimentado com castanha (P < 0,05). Não houve efeito da inclusão do farelo de castanha sobre o ganho de peso, rendimento de carcaça, desenvolvimento dos órgãos sexuais e parâmetros seminais dos animais. No entanto, a inclusão do farelo de castanha de caju esteve associada à expressão de proteínas seminais e espermáticas. Sete spots proteicos presentes nos mapas de plasma seminal foram expressos diferencialmente entre os grupos castanha e controle (P < 0,05) e um spot proteico (21,8 kDa; pI: 5) correlacionou-se negativamente com o vigor espermático (r = -0,49; P<0,05). Adicionalmente, sete spots proteicos dos géis com proteinas da membrana dos espermatozoides também diferiram entre os carneiros alimentados com e sem farelo de castanha de caju (P < 0,05). Conclui-se, portanto, que a inclusão de 13% de farelo de castanha de caju na dieta altera a expressão de proteínas seminais e espermáticas em ovinos.
Medeiros, Coralia. "Membrane modifications in bovine sperm during capacitation." 1994. http://catalog.hathitrust.org/api/volumes/oclc/33064256.html.
Full textTypescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 108-127).
Ting-wei, Jhan, and 詹庭威. "The possibility of producin treansgenic porcine embryos with membrane-damaged sperm by intracytoplasmic sperm injection." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/30768347202620347473.
Full text國立宜蘭大學
動物科技學系碩士班
97
The method of sperm-mediated gene transfer is a simple manipulation and costs low, however, direct fertilization in vitro with sperm carrying exogenous gene is difficult to achieve the transgenic animals by polyspermy. The ICSI technique could effectively overcomes the obstacle. Making damage on the sperm membrane was benefit to transgenic efficiency because of enhances the binding of exogenous gene, but the damage treatment on sperm would decrease the sperm motility and influence the fertilization ability. It must ensure normal fertilization by ICSI. The present study was to investigate the exogenous DNA-binding site, binding rate and copies in boar sperm. Further we evaluated the effect of making damage on the sperm membrane by frozen-thawed and dithiothreitol(DTT)co-incubation treatment on the exogenous gene-binding amount of sperm. Finally we used the technology of intracytoplasmic sperm injection combined with sperm vector to inject sperm bearing EGFP into the in vitro maturated porcine oocytes. The formation of pronuclear, fertilization and embryos development were observed to estimate the possibility of producing porcine embryos expressing green fluorescence. The highest survival rate (100%) of oocytes was the group of sperm capacitated within 5 hours combined with the oocytes in vitro maturated for 48 hours following ICSI. That was higher than the group of sperm capacitated within 5 hours combined with the oocytes maturated for 44 hours (86.7%) significantly, but there was no significantly difference with other 2 groups. Just for the rate of activation, the group of sperm capacitated within 1 hour combined with the oocytes maturated for 44 hour oocytes is the highest (80.1%) and higher than the group of sperm capacitated within 5 hour combined with in vitro maturated 44 hour oocytes (41.6%) significantly, but there was no significantly difference with other groups. However, the rate of male pronuclear formation and sperm decondensation were not influenced by the time of oocytes maturation and sperm capacitation. The boar sperm was co-incubated with FITC-dUTP DNA probe and observed the fluorescence on post-acrosome under microscope. The sperm bearing exogenous DNA after co-incubated with EGFP were estimated by real time PCR. The result showed that physical treatment of frozen-thawed enhanced EGFP-binding copies but chemical treatment of DTT co-incubation decreased the binding ability significantly. The EGFP fragments were electroporated into the cumulus cells to confirm that expressed normally. Further the oocytes were injected the frozen-thawed sperm that co-incubated with EGFP previously, the rate of male pronuclear formation or decondensed sperm head, normal fertilization were all lower than the group of DTT co-incubation. The rate of cleavage at 48 hour following ICSI were no significantly difference in the two group. We never obtained any porcine embryos of expressing green fluorescence.
"The role of membrane character in human sperm function." Tulane University, 1994.
Find full textacase@tulane.edu
Wrench, Nicola. "Effect of season on sperm membrane protein 22 and selected mRNAs in fresh and cryopreserved stallion sperm." 2007. http://www.lib.ncsu.edu/theses/available/etd-11092007-124518/unrestricted/etd.pdf.
Full textLin, Ting-Wei, and 林廷維. "Quantitative Phosphoproteomics Profiling of the Mouse Sperm Membrane Protein During Capacitation." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/19675907050990599867.
Full text國立臺灣海洋大學
生物科技研究所
96
After ejaculation, sperm are able to move actively but lack fertilizing competence. Capacitation is an important physiological pre-requisite before the sperm cells can acrosome react and fertilize the oocyte. Several studies suggested that capacitation is associated with phosphorylation of membrane proteins. However, the quantitation and qualification of membrane protein phosphorylation during capacitation is still less understood. To identify protein phosphorylation during capacitation, detergent-base partition coupled with tube-gel digestion and tandem mass spectrometry (MS/MS) was performed. In addition immobilized metal affinity chromatography (IMAC) was used to investigate phosphorylation site in whole protein digests before and after capacitated mouse sperm. After the purification of membrane proteins, about 250 proteins were identified and about 80% of the identified proteins were membrane-associated. After the phosphopeptide purification followed by LC-MS analysis, phosphorylation site for the capacitated and noncapacitated sperm proteins were identified and quantified. Using hexokinase 1 as an internal control, about 27 phosphorylation sites were identified to be increased in phosphorylation levels during capacitation. This approach provides a site-specific quantitation result for the protein phosphorylation during sperm capacitation. The results will help to improve our understanding of the pin-point protein phosphorylation that associates with hyperactive motility and redistribution of the membrane components during capacitation.
Figueiredo, Maria Inês Lopes. "Investigation of Changes in Stallion Sperm Mitochondrial Membrane Potential During Storage." Master's thesis, 2016. https://hdl.handle.net/10216/83887.
Full textFigueiredo, Maria Inês Lopes. "Investigation of Changes in Stallion Sperm Mitochondrial Membrane Potential During Storage." Dissertação, 2016. https://repositorio-aberto.up.pt/handle/10216/83887.
Full textStump, Karen Elizabeth. "Evaluation of Stallion Sperm Membrane Integrity Using Varied Flow Cytometer-Based Methodologies." Thesis, 2013. http://hdl.handle.net/1969.1/149554.
Full textLiu, Chih-Chun, and 劉志濬. "Assessment of Viability and Mitochondrial Membrane Sheath in Fresh and Frozen Boar Sperm." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/35653214082796715282.
Full text國立屏東科技大學
畜產系
91
Semen qulity evalution is the most important thing before artificial insemination. The objective of this study was to compare the vital stain of trypan blue and fluorometric technique to assess different living/killed ratio sperm of viability and mitochondrial membran sheath and utilization of fluorometric technique to estimate frozen-thawed sperm and different stage of frozen-thawed sperm viability. The parameters of motility, viability and mitochandrial membrane sheath of three treatments (fresh, killed and 1:1 ration sperm) were estimated by Trypan blue, SYBR-14/PI, Hoechst 33258 and Rhodamine 123. All of the parameters in three treament were significantly different (p<0.05). Trypan blue, Hoechst 33258 and Rhodamine 123 with SYBR-14/PI were positive correlation (0.96, 0.95 and 0.96). Correlation coefficient among motility, Trypam blue, Hoechst 33258, SYBR-14/PI and Rhodamine 123 were 0.87, 0.86, 0.85 and 0.93, respectively. Trypan blue show almost the same pattern with fluorometric technique and those stains can hightly correlation with motility. 5 ml and 0.5 ml straw were used for freezing package, after thawed 0.5ml straw have better frozen-thawed sperm motility comparison with 5 ml straw (p<0.05), but after 3 hr and 6 hr the motility between 5ml and 0.5ml straw were no significant difference. Estimated frozen-thawed sperm cell membrance status by Hoechst 33258, SYBR-14/PI and Rhodamine 123, those were no significant difference. Different stage among sperm motility and viability at frozen-thawed procedure, the sperm motility between fresh diluted and diluted with first stage freeze diluter were no significant difference, however, the motility decrease after diluted second stage freeze diluter (p<0.05). The sperm viability decrease after diluted with first stage freeze diluter, between first and second stage freeze diluter were no significant difference. Storage at 25℃ after 24hr the sperm motility and viability at different stages decreased almost the same with 0hr, but the motility drop obviously. The sperm cell membrane damaged at all stage in frozen-thawed procedure, after 24hr storage sperm motility drop more than membrane integrity. To estimate sperm viability by vital stain trypan blue is unhandy, fluorometric technique may replace it and empolyed for estimating sperm cell membrane integrity, found out that membrane integrity would be injured at each stage in frozen procedure, and result between 5 ml and 0.5 ml straw is similar except the sperm motility of frozen-thawed at 0 hr.
Foster, Mary L. "Comparison of Methods for Assessing Viability of Equine Spermatozoa and Effects of Seminal Plasma on Viability and Motion Characteristics of Equine Spermatozoa." 2009. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7469.
Full textWen, Chia-Wei, and 温家偉. "Using mouse Izumo1 as a model to study the phosphoproteome of sperm membrane after capacitation." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/34387185548412185658.
Full text國立臺灣海洋大學
生物科技研究所
98
Mammalian spermatozoa are unable to fertilize eggs immediately after ejaculation until they undergo a regulatory procedure named capacitation. Although many attempts, there are still many molecular mechanisms of capacitaion remain unclear. Since mature spermatozoa could only adjust their physiological condition through post-translational modifications, especially phosphorylation, we applied a quantitative phosphoproteomic platform, including detergent enrichment of membrane proteins, IMAC chromatography enrichment of phosphopeptides, and LC-MS/MS analysis for globally comparing of capacitated and non-capacitated mouse sperm membrane to identify important fertilization related molecules. Our data indicates that at least 11 proteins increased their phosphorylation level more than 2 folds after capacitation. By using GO algorism, all of the 11 proteins are annotated as membrane associated. Two previous known sperm-egg essential proteins, Izumo1 and SPACA1, were found to increase their phosphorylation level after capacitation. The others are known as membrane-integrated enzymes, transporters or receptors, which may play roles in fertilization awaits further investigation. To evaluate our MS data, we also performed in vitro kinase assay with recombinant Izumo1 (GST-Izumo1) in capacitated or non-capacitated mouse sperm lysate. We found it also increased phosphorylation in capacitated sperm lysate and the phosphorylation site was in accord with endogenous Izumo1. Further, we made a mutant Izumo1 devoid of the phosphorylation site. By comparing mutant and wild-type GST-Izumo1, we found that a potential Izumo1 associated protein binding was also weakened as the phosphorylation level decreased. By using multiple sequence alignment, it is found that the phosphorylation sites of mouse Izumo1 are not observed in the rat Izumo1. However, we found that the rat Izumo1 recombinant protein is still phosphorylated in the capacitated mouse sperm lysate and the phosphorylation sites were in accord with the endogenous rat Izumo1. To sum up, our work provided a powerful tool for studying the molecular mechanism of sperm capacitation and fertilization
Khunsook, Sumpars. "Purification and characterization of human sperm plasma membrane-associated and human seminal fluid [alpha]-L-fucosidases /." Diss., 2001. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3036265.
Full textShalaweh, Salem. "Effect of cissampelos capensis rhizome extract on human sperm capacitation and acrosome reaction." 2013. http://hdl.handle.net/11394/3881.
Full textCissampelos capensis, is commonly known by the Afrikaans name ‟dawidjies” or ‟dawidjieswortel”. C. capensis is the most important and best known medicinal plant of the family Menispermaceae used by the Khoisan and other rural people in the western regions of South Africa. Among numerous other ailments, it is traditionally taken to treat male fertility problems. Yet, no studies have investigated the effects of this plant or its extracts on human spermatozoa. The aim of study was to investigate the effects of C. capensis rhizome extracts on sperm function.
Yu, YANG. "THE IDENTIFICATION AND CHARACTERIZATION OF AN INNER ACROSOMAL MEMBRANE ASSOCIATED PROTEIN, IAM38, RESPONSIBLE FOR SECONDARY SPERM-ZONA BINDING DURING FERTILIZATION." Thesis, 2008. http://hdl.handle.net/1974/1589.
Full textThesis (Ph.D, Anatomy & Cell Biology) -- Queen's University, 2008-11-27 15:33:50.226
Cheng, Chiu-Hung, and 鄭秋虹. "Research of using opposite-sex immunization to screen sex-specific proteins from X- and Y-sperm plasma membrane of pigs." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/61379468661546998575.
Full text國立中興大學
獸醫學系
92
Biotechnology for pre-selecting gender of one’s offspring is much worth business investment. However, there is no effective, convenient and safe means to separate X and Y sperm for pre-selecting gender to date. One of the feasible ways is to use a non-invasive and immunological method to screen X or Y sperm out. Based on the Ohno’s law that sex-specific proteins (SSPs) are evolutionarily more highly conserved than non-SSPs, separation of SSPs from X or Y sperm creates a foundation for further immunological selection of X or Y sperm. Opposite-sex immunizations (antigens: swine sperm membrane crude proteins) of rabbits was applied to produce the corresponding antibodies for each sex. ELISA confirmed the paramount of the raising antibodies. SDS-PAGE and Western blotting analysis revealed comparable membrane proteins patterns in each sex antibody preparations. Sex-specific affinity columns prepared from the antibodies produced by each sex were used to purify SSPs for three cycles. Preliminary results demonstrated that two obvious X-SSPs bands (13.6 and 72.2 kDa) were detected by the one-dimensional electrophoresis analysis. However, these results were not consistently presented under same preparations (n=3). Further, with the application of high resolution two-dimensional electrophoresis and western blotting (antibodies of each sex), purified X-SSPs (app. 102 kDa) obtained from three-cycles affinity column chromatography, was clearly observed. In conclusion, this study has shown the presence of X-SSPs. But, Y-SSps appears to be imperceptible. Nevertheless, the result of present study indicates the feasibility of immunological sperm sexing techniques. Further investigation should be toward on the immunoaffinity enrichment for SSPs and the protein identification.
Tan, Wenxian active 21st century. "Elucidating the signal cascades induced by progestins that mediate sperm hypermotility in Atlantic croaker (Micropogonias undulatus) and southern flounder (Paralichthys lethostigma)." Thesis, 2013. http://hdl.handle.net/2152/28716.
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Canvin, Andrew T. "Factors affecting the fluidity of boar sperm membranes." 1988. http://hdl.handle.net/1993/16621.
Full textSpringsguth, Hans Christopher. "Mechanismen und Bedeutung der aktivierten Apoptosekaskade in humanen Spermatozoen." Doctoral thesis, 2015. https://ul.qucosa.de/id/qucosa%3A14133.
Full textBašus, Kryštof. "Monitorování dynamiky proteinových sítí: role FcRL proteinů při interakci membrány spermie a vajíčka." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-435880.
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