Relevant bibliographies by topics / Sperm

Academic literature on the topic 'Sperm'

Author: Grafiati
Published: 4 June 2021
Last updated: 18 May 2024

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Journal articles on the topic "Sperm"

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Xie, Lan, Rui Ma, Chao Han, Kai Su, Qiufang Zhang, Tian Qiu, Lei Wang, et al. "Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-Based Device." Clinical Chemistry 56, no. 8 (August 1, 2010): 1270–78. http://dx.doi.org/10.1373/clinchem.2010.146902.

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BACKGROUND Sperm screening is an essential step in in vitro fertilization (IVF) procedures. The swim-up method, an assay for sperm motility, is used clinically to select the ideal sperm for subsequent manipulation. However, additional parameters, including acrosome reaction capability, chemotaxis, and thermotaxis, are also important indicators of mammalian sperm health. To monitor both sperm motility and chemotaxis simultaneously during sperm screening, we designed and constructed a microdevice comprising a straight channel connected with a bibranch channel that mimics the mammalian female reproductive tract. METHODS The width and length of the straight channel were optimized to select the motile sperms. We selectively cultured cumulus cells in the bibranch channel to generate a chemoattractant-forming chemical gradient. Sperm chemotaxis was represented by the ratio of the sperm swimming toward different branches. RESULTS The percentage of motile sperms improved from 58.5% (3.8%) to 82.6% (2.9%) by a straight channel 7 mm in length and 1 mm in width. About 10% of sperms were found to be chemotactically responsive in our experiment, which is consistent with previous studies. CONCLUSIONS For the first time, we achieved the combined evaluation of both sperm motility and chemotaxis. The motile and chemotactically responsive sperms can easily be enriched on a lab-on-a-chip device to improve IVF outcome.
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S. Vishwekar, Pallavi, Nikita Lad, Mamta Shivtare, and Pradnya Shetty. "ICSI outcome in surgically retrieved sperm compared with ejaculated sperm control." International Journal of Reproduction, Contraception, Obstetrics and Gynecology 8, no. 3 (February 26, 2019): 869. http://dx.doi.org/10.18203/2320-1770.ijrcog20190847.

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Background: Globally, the prevalence of infertility is around 10% of the total population. 30% of these have male factor infertility. Azoospermia is found in 1% of men, in 20% of which, the etiology is a bilateral obstruction of the male genital tract while others have non obstructive azoospermia. In azoospermic men sperms are microsurgically retrieved from epididymis and testes by TESA and PESA respectively. The aim of this study was to evaluate the outcomes of intracytoplasmic sperm injection ICSI using surgically retrieved sperm of azoospermic men either obstructive or nonobstructive and to compare it with ejaculated sperms in men having severe oligospermia.Methods: This was retrospective cohort study conducted based on the data collected from our reproductive endocrinology and infertility unit, 126 ICSI cycles performed during the period of 5 years were taken and divided into two groups, one with patients having ejaculated sperms with oligospermia and other group with patients who had surgically retrieved normal sperms due to azoospermia. Outcome of these ICSI cycles included fertilization, cleavage, biochemical and clinical pregnancy was assessed.Results: In present study it was found that ICSI outcome was comparable in both the groups with ejaculated sperm and surgically retrieved sperm as fertilization rate (72% vs 65%), Implantation Rate (58 vs 51%), clinical pregnancy rate (CPR) (51% vs 44.82%) observed with ejaculated or retrieved sperm group respectively showed no statistical difference.Conclusions: Present study shows that minimally invasive techniques of PESA and TESA can be successfully performed to retrieve sperm for ICSI in the treatment of azoospermic men which gives them the chance to father their biological child. The result of this study indicates that treatment outcomes of PESA/TESA-ICSI cycles compare favourably with that of ICSI using ejaculated sperm.
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Kaliky, Nunun Ainun Putri Sari Banun, Mia Setiawati, Odang Carman, and Nur Bambang Priyo Utomo. "Effect of zinc (Zn) supplementation on quality and quantity of striped catfish Pangasianodon hypophthalmus sperm." Jurnal Akuakultur Indonesia 18, no. 1 (January 18, 2019): 46–53. http://dx.doi.org/10.19027/jai.18.1.46-53.

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ABSTRACT This study aimed to evaluate the effects of Zinc (Zn) supplementation on the quality and quantity of striped catfish sperm. Experimental design for this study was a complete randomized design with five treatments and five replications. Male broods fed with Zn supplementation for eight weeks. The Zn supplemented into the fish diet at different concentrations (0, 50, 100, 150 and 200 mg/kg of feed). The results showed that Zn supplementation could improve the quality and quantity of striped catfish sperm. The treatments also showed significant effects on semen volume, sperm motility, sperm viability, and sperm concentration (P<0.05). Zn supplementation at a dose of 200 mg/kg feed demonstrated the best result has indicated by enhancement of quality and quantity of striped catfish sperm, increasing 51% of the volume, 11.6% of motility, 5.81% of viability, 54.1% of concentrations. The results suggested that Zn played an important role in improving reproductive performances of male striped catfish reproduction. Keywords: quality of sperm, a quantity of sperm, striped catfish, supplementation zinc ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi Zinc (Zn) terhadap kualitas dan kuantitas sperma ikan patinPangasianodon hypophthalmus. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan danlima ulangan. Induk jantan diberi pakan dengan suplementasi Zn selama 8 minggu. Zn disuplementasikan dengan dosis berbeda (0, 50, 100, 150 dan 200 mg/kg pakan). Hasil penelitian menunjukkan bahwa suplementasi Zn dapat meningkatkan kualitas dan kuantitas sperma ikan patin sehingga berpengaruh signifikan terhadap volume semen, motilitas, viabilitas dan konsentrasi sperma (P<0,05). Suplementasi Zn pada dosis pakan 200 mg/kg menunjukkan hasil terbaik yang ditunjukkan oleh peningkatan kualitas dan kuantitas sperma ikan patin 51% volume; 11,6% motilitas; 5,81% viabilitas; 54,1% konsentrasi sperma. Hasil penelitian ini menunjukkan bahwa Zn memainkan peran penting dalam meningkatkan reproduksi ikan patin. Kata kunci: kualitas dan kuantitas sperma, Ikan patin, suplementasi Zn
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Salama, Nader, and Omer Sirelkhatim Hassan. "Staged Laboratory Processing of Testicular Tissue in Non-Obstructive Azoospermia May Rescue Retrieving an Existing Sperm: A Case Report and Literature Review." Clinical Medicine Insights: Case Reports 16 (January 2023): 117954762311783. http://dx.doi.org/10.1177/11795476231178353.

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Non-obstructive azoospermia (NOA) is the most difficult form of male infertility to manage. It usually requires sperm retrieval from the testis, which is most challenging due to sperm rarity. Here, we describe the recovery of testicular sperms that had been missing and whose original retrieval results were negative. Salvage microsurgical testicular sperm extraction and sperm testing were performed on a 36-year-old male with NOA. Neither in the operation room nor after an inspection in the embryology laboratory were any sperm detected. The obtained tissue was advised to be frozen because the patient data and surgical microscopy predicted a favorable outcome, and the tissue processing was done in an inappropriate environment. About 1 month later, the specimen was thawed, crushed, and re-examined. Successful oocyte fertilization resulted from an effective detection of sperms and their direction to intra-cytoplasmic sperm injection. This is the first case report that, to the best of our knowledge, describes the stepwise laboratory processing of testicular tissue and its capacity to recover lost sperms in challenging NOA cases and under less-than-ideal working conditions.
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Sudipta Chowdhury and Samarendra Nath Banerjee. "Genotoxic activity of betel nut on germinal cell in Sarcoma 180 ascites tumour bearing male mice." GSC Biological and Pharmaceutical Sciences 16, no. 2 (August 30, 2021): 171–81. http://dx.doi.org/10.30574/gscbps.2021.16.2.0235.

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The genotoxicity of the ethanolic extract of betel nut was evaluated using sarcoma 180 tumour bearing mouse considering sperm motility, sperm viability, biochemical estimation of fructose in seminal fluid and sperm head morphology assays. Sperm head morphology was studied by H-E staining and Toluidine blue staining method. But Toluidine blue staining method is a reliable method to evaluate the DNA damage of sperms. Ethanolic BNE (betel nut extract) can suppress the percentage of sperm motility, sperm viability and seminal fructose level. In addition, it can also enhance the percentage of DNA damaged sperms. Moreover, histological sections of testes have been studied in control and BNE treated sarcoma 180 tumour bearing mice to highlight the potential toxic effect of BNE. The significant decreasing rate of seminal fructose concentration, sperm motility as well as viability and increasing rate of sperm head abnormality in different doses of treated series may be as a result of different toxic alkaloid ingredients present in BNE. Therefore, the results showed the potential of the BNE to induce different types of germ cell abnormalities in tumour bearing male mice.
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Akbar, Fahmi, Agus Oman Sudrajat, and Siti Subaidah. "Sperm quality of Litopenaeus vannamei broostock injected by PMSG and antidopamin." Jurnal Akuakultur Indonesia 14, no. 2 (October 15, 2015): 98. http://dx.doi.org/10.19027/jai.14.98-103.

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<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">The important role in determining the productivity of shrimp was the quality and quantity of shrimp sperm. The decreasing of hatching rate was predicted as the effect of the decreasing quality of sperm. It then could influence the number and quality of naupli produced. Hormonal induction of maturation is one of alternative solution that can improve shrimp sperm quality. This study was conducted to examine the effect of pregnant mare serum gonadotropin (PMSG) and antidopamine (AD) injection on white shrimp <em>Litopenaeus vannamei</em> sperm quality. This research consisted of six treatments which were treatment without eyestalk ablation, eyestalk ablation, and premix PMSG hormone, and AD at the dose of 0.1 mL/kg, 0.25 mL/kg, 0.5 mL/kg, and 1 mL/kg. The observed parameters were sperm count and percentage of normal and abnormal sperm. The results showed that PMSG hormone and AD injection could improve sperm quality of <em>L. vannamei</em> shrimp. Hormone at the dose of 0.25 mL/kg and 0.5 mL/kg were the optimal doses to increase sperm count and the percentage of normal sperm, also to lower the percentage of abnormal sperm.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keyword: PMSG, AD, sperm quality, <em>Litopenaeus vannamei</em></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kuantitas dan kualitas sperma udang jantan sangat berperan penting dalam menentukan produktivitas udang. Terjadinya penurunan daya tetas telur udang diduga karena terjadinya penurunan kualitas sperma. Hal ini berpengaruh terhadap jumlah dan kualitas nauplius yang diproduksi. Induksi maturasi secara hormonal merupakan salah satu alternatif yang dapat meningkatkan kualitas sperma udang. Penelitian ini dilakukan untuk mengkaji pengaruh penyuntikan <em>pregnant mare serum gonadotropin</em> (PMSG) dan antidopamin (AD) terhadap kualitas sperma udang vaname <em>Litopenaeus vannamei</em>. Penelitian terdiri atas enam perlakuan, yaitu perlakuan tanpa ablasi mata, ablasi mata, dan injeksi dengan premix hormon PMSG dan AD dosis 0,1 mL/kg, 0,25 mL/kg, 0,5 mL/kg, dan 1 mL/kg. Parameter yang diamati jumlah sperma, persentase sperma normal dan abnormal. Hasil penelitian menunjukkan penyuntikan hormon PMSG dan AD dapat meningkatkan kualitas sperma udang <em>L. vannamei</em>. Hormon dosis 0,25 mL/kg dan 0,5 mL/kg merupakan dosis optimal dalam meningkatkan jumlah sperma dan persentase sperma normal, serta mengurangi persentase sperma abnormal.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: PMSG, AD, kualitas sperma, <em>Litopenaeus vannamei</em></p><p> </p>
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Dewi, Raden Roro Sri Pudji Sinarni, Alimuddin Alimuddin, Agus Oman Sudrajat, Komar Sumantadinata, and Sularto Sularto. "OPTIMAL ELECTROPORATION CONDITION FOR SPERM MEDIATED GENE TRANSFER IN STRIPPED CATFISH (Pangasionodon hypophthalmus)." Indonesian Aquaculture Journal 5, no. 1 (June 30, 2010): 1. http://dx.doi.org/10.15578/iaj.5.1.2010.1-10.

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The success of transgenic fish production has been achieved through eggs fertilization using electroporated sperms carrying exogenous DNA. This study was conducted in order to obtain the optimal electroporation condition for stripped catfish sperm. A plasmid containing green fluorescent protein (GFP) gene driven by carp β-actin promoter was transferred into sperm using electrophoresis method towards transgenic stripped catfish (Pangasionodon hypophthalmus) production. Electroporation was carried out using square wave shock with pulse length of 30 ms and pulse interval of 0.1 sec. Treatments are combination between voltage (50 V, 75 V, and 100 V) and pulse number (1 and 3). Exogenous DNA concentration used was 10 μg/mL of Tris-EDTA. Results showed that increasing the voltage from 50 to 100 decreased sperm motility, while pulse number did not affect sperm motility. Voltage of 50 gave the best motility of sperm, although sperm viability relatively similar between treatments and control except at 100 V with 3 pulses number. Further, electroporation-treated sperms were able to fertilize eggs. Higher hatching rate of eggs was obtained in electroporation treatment at 50 V with pulse number of 1 and 3. The persistence of transferred GFP was detected in electroporated and incubated sperms (control). However, GFP was only detected in larvae from eggs that were fertilized by electroporated sperm. Thus, electroporation could be applied to produce transgenic stripped catfish.
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Rahayu, Vitaloka Guci, and Evi Hanizar. "The Effect of Lemon (Citrus limon) Extracts On The Quantity and Quality of Mice (Mus musculus) Sperm." Elkawnie 7, no. 2 (January 17, 2022): 300. http://dx.doi.org/10.22373/ekw.v7i2.9389.

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Abstract: Vitamin C has been proved as a nutrient to improve the quality of sperm. Society believed that consuming the lemons could potentially enhance the sperm quality of humans. However, the appropriate concentration should be well studied to obtain the optimum concentration to improve the sperm quality and quantity. The present research tried to provide information on how lemon could improve the sperm quality by designing a true experimental using a series concentration of lemon extract (25%, 50% and 75 % concentrations) given to the male mice (Mus musculus). The investigation was made by giving the lemon treatment three times a day for 5 weeks. To investigate the effect of lemon extract, the mice sperm were taken from the epididymis and observed using a multimedia microscope and counted using Neubauer’s counting rooms, while motility and morphology were observed using object-glass. The result showed that the high concentration of lemon could not provide the greatest improvement of sperm quality and quantity. The optimum condition was seen in 25% of lemon extract, where the increase of lemon concentration suppressed the lemon improvement effect, which reduced the sperm quality and quantity. However, the improvement was still made if the result was compared to control, meaning consuming lemon was better than consuming any lemon treatment. The result was in accordance with quality improvement of sperm, where 25% of lemon concentration treatment provided the highest average motility and normal morphology of sperm. However, the high concentration of lemon extract (50% and 75% of lemon concentrations) provided a lower effect due to the adverse effect. The result proved that lemon could be used to boost the quality and quantity of sperm in an appropriate concentration where the excess lemon extract could reduce the effect of lemon in improving sperm quality and quantity.Abstrak: Vitamin C telah terbukti sebagai nutrisi untuk meningkatkan kualitas sperma dimana masyarakat percaya bahwa mengkonsumsi lemon berpotensi meningkatkan kualitas sperma manusia. Namun, konsentrasi yang tepat harus dipelajari dengan baik untuk mendapatkan konsentrasi yang optimal untuk meningkatkan kualitas dan kuantitas sperma. Penelitian ini mencoba memberikan informasi bagaimana lemon dapat meningkatkan kualitas sperma dengan merancang eksperimen nyata menggunakan serangkaian konsentrasi ekstrak lemon (konsentrasi 25%, 50% dan 75%) yang diberikan kepada mencit jantan (Mus musculus). Penyelidikan dilakukan dengan memberikan ekstrak lemon sebanyak tiga kali dalam sehari selama 5 minggu. Untuk mengetahui pengaruh ekstrak lemon, sperma mencit diambil dari epididimis dan diamati menggunakan mikroskop multimedia dan dihitung menggunakan kamar hitung Neubauer, sedangkan motilitas dan morfologi diamati menggunakan kaca objek. Hasil penelitian menunjukkan bahwa konsentrasi lemon yang tinggi tidak dapat memberikan peningkatan kualitas dan kuantitas sperma yang tertinggi. Kondisi optimum terlihat pada ekstrak lemon 25% dimana peningkatan konsentrasi lemon menekan efek perbaikan lemon yang menurunkan kualitas dan kuantitas sperma. Namun perbaikan tetap dilakukan jika hasilnya dibandingkan dengan kontrol, artinya mengkonsumsi lemon lebih baik daripada tanpa mengkonsumsi lemon. Hasil tersebut sesuai dengan peningkatan kualitas sperma dimana perlakuan konsentrasi lemon 25% memberikan rata-rata motilitas dan morfologi sperma yang normal paling tinggi. Namun, konsentrasi tinggi ekstrak lemon (50% dan 75% konsentrasi lemon) memberikan efek yang lebih rendah karena efek samping. Hasil penelitian membuktikan bahwa lemon dapat digunakan untuk meningkatkan kualitas dan kuantitas sperma dalam konsentrasi yang sesuai dimana kelebihan ekstrak lemon dapat mengurangi efek lemon dalam meningkatkan kualitas dan kuantitas sperma.
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SWAIN, DILIP KUMAR, PRASANT SWARNKAR, JITENDER KUMAR, and SARVAJEET YADAV. "Evaluation of in vitro longevity of caprine cauda epididymal sperms at different storage intervals of time." Indian Journal of Animal Sciences 82, no. 11 (November 20, 2012): 1347–50. http://dx.doi.org/10.56093/ijans.v82i11.25144.

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The present study was designed to evaluate the effect of both temperature and storage time interval on the cauda epididymal sperm quality in bucks. The testes collected in normal saline at 4°C were exhibited improved sperm attributes at all periods of storage temperature as compared to the testes collected in normal saline at room temperature as well as preserved at the same temperature. In both cases, with the passage of time of storage the sperm features started deteriorating. That is why the study recommended that to maintain the sperm attributes for a longer period of storage, suitable antioxidants and amino acids may be supplemented. The sperms should be isolated as soon as possible after the collection of the testes to maintain the integrity and quality of the sperm from the cauda. It was evident that, as soon as possible, the sperms from the cauda should be recovered as well as storage temperature should be lesser than 4°C. Suitable strategies should be developed to cryopreserve the cauda epididymal sperms.
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Tian, Fang-Bao, and Li Wang. "Numerical Modeling of Sperm Swimming." Fluids 6, no. 2 (February 7, 2021): 73. http://dx.doi.org/10.3390/fluids6020073.

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Due to rising human infertility, sperm motility has been an important subject. Among the hundreds of millions of sperms on the journey up the oviducts, only a few excellent travelers will reach the eggs. This journey is affected by many factors, some of which include sperm quality, sperm density, fluid rheology and chemotaxis. In addition, the sperm swimming through different body tracks and fluids involves complex sperm flagellar, complex fluid environment, and multi-sperm and sperm-wall interactions. Therefore, this topic has generated substantial research interest. In this paper, we present a review of computational studies on sperm swimming from an engineering perspective with focus on both simplified theoretical methods and fluid–structure interaction methods. Several open issues in this field are highlighted.
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Dissertations / Theses on the topic "Sperm"

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Bennett, Monique. "Marine invertebrate sperm: Assessment of sperm quality using computer-aided sperm analysis." University of Western Cape, 2020. http://hdl.handle.net/11394/7747.

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Williams, Katrina. "Sperm-CMV interactions : implications for sperm donor recruitment." Thesis, University of Sheffield, 2016. http://etheses.whiterose.ac.uk/15713/.

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Human Cytomegalovirus (CMV) is a common herpesvirus found in 60% of the population. Normally, it poses no risk, however it can have consequences for unborn babies. This is of concern when donor sperm is used in assisted conception, as CMV is present in semen. The risk of transmission from a positive donor is unclear, as it is not known if sperm can act as a vector for transmission. Additionally, this raises questions about whether CMV might affect sperm function. The hypothesis for this study is that CMV will interact with human sperm and alter sperm function and that sperm will act as a vector for viral transmission. A survey was conducted to examine how fertility clinics were screening for CMV in sperm donors. This survey found that the majority of UK clinics are screening for CMV in sperm donors in the manner recommended by current guidelines but that the requirement to screen for CMV is causing problems in clinics with regards to sperm donor supply. Fortunately, this thesis has shown that sperm washing by density gradient centrifugation is mostly effective at removing CMV from semen samples infected in vitro, with CMV (AD169) grown in the laboratory, and in naturally infected samples. This presents a possible approach for alleviating some of the problems relating to CMV infection in sperm donors in UK fertility clinics. However, co-incubation with CMV has no effect on any of the sperm function parameters tested in this thesis, including, motility, viability, acrosome reaction, tyrosine phosphorylation and levels of DNA damage. In conclusion, this thesis has highlighted problems with the current approach to screening and managing CMV infection in sperm donors but has provided evidence to show that there could be a simple solution to the problem. No effect on sperm function was observed, but this does not rule out a direct interaction between CMV and sperm. Overall, this thesis shows that fertility clinics should be concerned about CMV infection in sperm donors, but that simple steps could be taken to alleviate the current problems clinics are experiencing.
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Maree, Liana. "Sperm mitochondria: Species specificity and relationships to sperm morphometric features and sperm function in selected mammalian species." Thesis, University of the Western Cape, 2011. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1728_1363788268.

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Numerous studies on mammalian spermatozoa have reported large variations in the dimensions of the main sperm structural components, namely the head, midpiece and flagellum. These variations in sperm architecture are believed to be adaptations for functioning of spermatozoa in complex environments outside the male reproductive system. The midpiece of the mammalian 
permatozoon contains a varied number of mitochondria, but the reason for the marked difference in the size and structure of this sperm component is not clear. This study 
confirmed the variations in the sperm morphometry of seven selected mammalian species and revealed unique features of the sperm midpiece and sperm mitochondria of these seven species. Evaluation of several sperm kinematic parameters revealed the unique swimming characteristics of the different spermatozoa. The importance of using standardized motility 
parameters was highlighted as well as the assessment of different subpopulations of spermatozoa in order to produce more reliable and comparable data. Investigating the role of sperm mitochondria in human sperm 
metabolism indicated that these organelles are related to sperm function in terms of sperm motility. Furthermore, it was suggested that glycolysis and mitochondrial respiration are linked processes and that both are important for the maintenance of human sperm motility. By optimizing and employing standardized experimental procedures and analysis techniques, this study was 
able to confirm the species specificity of almost all the sperm parameters evaluated, while also elucidating the phylogenetic relatedness of the non-human primate species. In conclusion, the present study has confirmed that the various midpiece morphometry parameters are related to the remaining sperm morphometry parameters as well as to the sperm kinematic parameters. 
These proposed associations between the various sperm parameters were used to explain the sperm velocity of two hypothetical and morphologically different sperm structures. Therefore, the results of the current study support the idea of co-evolution between sperm components in mammalian spermatozoa and propose that the midpiece morphometry parameters that are selected for in these spermatozoa are midpiece volume, total number of mitochondrial gyres, thickness of the mitochondrial sheath and mitochondrial height.

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Huang, Wenxin, and 黃聞馨. "Sperm fucosyltransferase-5 mediates the sperm-oviductal epithelial cell interaction to protect human sperm from oxidative damage." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/196485.

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Oxidative damage by reactive oxygen species (ROS) is a major cause of sperm dysfunction. Excessive ROS generation reduces fertilization and enhances DNA damage of spermatozoa. In mammals, including humans, oviduct functions as a sperm reservoir which is created by the binding of spermatozoa to the epithelial lining in the oviduct. Interaction between sperm and oviductal epithelial cells improves the fertilizing ability of and reduces chromatin damage in spermatozoa. However, the mechanism(s) by which spermatozoa-oviduct interaction producing these beneficial effects is unknown. One possibility is that oviduct protects spermatozoa from oxidative stress. The hypothesis of this project was that oviductal cell membrane proteins interact with spermatozoa to protect them from oxidative damage. Due to the limited availability of human oviductal tissue for research, an immortalized human oviductal epithelial cell line, OE-E6/E7, was used as a study model. The first objective examined the effect of OE-E6/E7 membrane proteins on human spermatozoa. The extracted OE-E6/E7 membrane proteins bound to sperm head and preferentially to uncapacitated sperm. Pretreatment with OE-E6/E7 membrane proteins significantly suppressed ROS-induced adverse effects in sperm motility, membrane integrity, DNA integrity, and intracellular ROS level. OE-E6/E7 membrane proteins also increased the endogenous enzyme activities of sperm superoxide dismutase (SOD) and glutathione peroxidase (GPx). Sperm fucosyltransferase-5 (sFUT5) is a membrane carbohydrate-binding protein on human sperm. The second objective investigated the involvement of sFUT5 in sperm-oviduct interaction. Purified sFUT5 bound to OE-E6/E7 cells and anti-FUT5 antibody inhibited this interaction. Pre-absorption of OE-E6/E7 membrane proteins with purified sFUT5 or blocking of sFUT5 on sperm with anti-FUT5 antibody significantly inhibited the protective effects of OE-E6/E7 membrane proteins against ROS-induced damages in spermatozoa. Asialofetuin, a reported sFUT5 substrate, can partly mimic the protective effect of OE-E6/E7 membrane proteins. Sperm processing in assisted reproductive technology (ART) treatment, including centrifugation and cryopreservation, has shown to induce ROS production and oxidative damage in sperm. The third objective investigated the possible use of OE-E6/E7 membrane proteins or asialofetuin as an antioxidant supplement during centrifugation and cryopreservation. No adverse effect on sperm functions was detected by centrifugation using our centrifugation protocols. OE-E6/E7 membrane proteins or asialofetuin pretreatment suppressed the cryopreservation-induced damage on sperm in terms of motility and DNA fragmentation. The fourth objective aimed to identify the sFUT5-interacting proteins from OE-E6/E7 membrane extracts. By using immuno-affinity chromatography and mass spectrometry analysis, cell adhesion molecule 4 (CADM4) was identified as a potential sFUT5-interacting protein. This result was further supported by co-immunoprecipitation, immunofluorescent staining and immunohistochemistry. CADM4 expression level was shown to be higher at follicular phase when compared to luteal phase of the menstrual cycle. In conclusion, this thesis demonstrated that oviductal epithelial cell membrane proteins bind to the human spermatozoa and protect them from ROS-induced damages in terms of motility, membrane integrity, and DNA integrity. sFUT5 mediates the spermatozoon-oviductal epithelial cell interaction and the beneficial effects of such interaction on the fertilizing ability of spermatozoa. Results from this study provide the potential use of sFUT5-interacting proteins to enhance the fertilization ability of human spermatozoa by protecting them from oxidative stress.
published_or_final_version
Obstetrics and Gynaecology
Doctoral
Doctor of Philosophy
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Achikanu, Cosmas Ezekaibeya. "Regulation of sperm motility by cell-signalling events in human sperm." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7907/.

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Ca²⁺ signals from activated Ca²⁺ channels (CatSper) and mobilisation of Ca²⁺ stores regulate human sperm cell behaviour as they ascend the female tract. I investigated the effects on human sperm [Ca²⁺]i and behaviour of CatSper channel activation (alkaline pH and progesterone) and Ca²⁺-store mobilisation (4-aminopyridine, thimerosal) using a fluorescence plate reader and CASA. Extracellular alkalinisation raised pH¬i (pHi = 6.9 and 7.2 at pHo7.4 and 8.5 respectively), caused tonic elevation of [Ca²⁺]i, which was partially inhibited by CatSper block and increased the proportion of hyperactivated cells (from 1.8±0.5 to 10.5±1.6%; n=34, P=1x10⁻⁷). Progesterone elevated [Ca²⁺]i but caused negligible hyperactivation. Co-application of these stimuli revealed little, if any, synergistic interaction. Ca²⁺-store mobilisation (4-aminopyridine) caused prolonged [Ca²⁺]i elevation and was associated with strong hyperactivation. Analysis of [Ca²⁺]i and hyperactivation data from 24 different conditions in this study showed a continuous relationship between [Ca²⁺]i and hyperactivation. The strong hyperactivating effect of store mobilisation (compared to CatSper activation) may reflect opening of store-operated channels. Human sperm behaviour assessed over a 180 s recording revealed regular ‘switching’ between progressive and various hyperactivated types. Mobilisation of Ca²⁺ stores potently increased hyperactivated behaviour and suppressed the rate of behavioural switching.
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Chang, Camacho Violeta Noemí. "Segmentation and classification of human sperm heads towards morphological sperm analysis." Tesis, Universidad de Chile, 2015. http://repositorio.uchile.cl/handle/2250/136250.

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Doctora en Ciencias, Mención Computación
La infertilidad es un problema clínico que afecta hasta a 15% de parejas en edad reproductiva, con implicancias tanto emocionales como fisiológicas. Un análisis de semen es el primer paso en la evaluación de una pareja infértil. El énfasis en identificar no sólo cabezas normales de espermatozoides sino también categorías de cabezas anormales puede tener una significativa utilidad clínica al decidir por un tratamiento de fertilidad. Esta tesis propone una nueva metodología para detectar, segmentar, caracterizar y clasificar cabezas de espermatozoides humanos, con el objetivo de facilitar el posterior análisis morfológico, para diagnósticos de fertilidad, toxicología reproductiva, investigación básica o estudios de salud pública. En la primera parte de este tesis, se ha tratado la detección y segmentación de cabezas de espermatozoides humanos. En este sentido, se propone un gold-standard para segmentación de espermatozoides construido con la cooperación de un experto referente en el área, para comparar métodos para detección y segmentación de espermatozoides. Además, se ha desarrollado un framework para la detección y segmentación de componentes de cabezas de espermatozoides humanos (incluyendo acrosoma y núcleo) que usa tres espacios de color además de técnicas de clustering y análisis estadístico del histograma. La evaluación experimental muestra que el método propuesto mejora el desempeño del estado del arte. Los resultados logran 98% de detección correcta a expensas de un número menor de falsos positivos, comparado con el estado del arte. Así mismo, los resultados de segmentación de cabeza, acrosoma y núcleo muestran más de 80% de solapamiento comparado con las máscaras de segmentación manual del gold-standard. En la segunda parte de esta tesis, el enfoque estuvo en la caracterización y clasificación de cabezas de espermatozoides humanos. Así, se introduce un gold-standard para clasificación de cabezas de espermatozoides humanos, construido con la colaboración de tres expertos referentes en área, y de acuerdo al criterio de la OMS. Además, se ha formulado un nuevo descriptor para cabezas de espermatozoides que, combinado con otros descriptores basados en forma, permite discriminar entre cabezas de espermatozoides normales y anormales, identificando cuatro tipos de cabezas anormales. También se propone un esquema de clasificación, que permite categorizar las cabezas de espermatozoides en 5 clases diferentes, según la OMS. La evaluación experimental muestra que el esquema propuesto tiene mejor desempeño que distintos clasificadores monolíticos, así como varios esquemas de clasificación en cascada que fueron diseñados en el contexto de esta investigación. Los resultados muestran más de 70% de clasificación correcta usando un dataset de total concordancia entre expertos del área.
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Mabotha, Luke Allen. "Evaluation of sperm functionality in non-human primates, focussing on sperm capacitation." University of the Western Cape, 2019. http://hdl.handle.net/11394/7027.

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Magister Scientiae (Medical Bioscience) - MSc(MBS)
The incidence of male infertility is increasing, with up to 50% of infertile males having “unexplained” (idiopathic) infertility. Newly developed molecular techniques have great value in detecting subtle causes of male infertility, as compared to idiopathic infertility which may be explained by standardizing and optimizing sperm functional and structural tests in non-human primate (NHP) sperm. The aim of the study was to evaluate sperm functionality utilizing the sperm of two NHP species, i.e.1) the rhesus monkey (Macaca mulatta) and 2) the vervet monkey (Chlorocebus aethiops), and further evaluate the effect of physiological media (including commonly used, and newly formulated sperm wash and sperm capacitating media) on NHP sperm functionality. Sperm functionality was evaluated by investigating the following sperm functions i.e.: sperm motility, vitality, acrosome reaction (AR), hyperactivation, and mitochondrial membrane potential (MMP). Sperm functional tests included computer-aided semen analysis (CASA), motility analysis, BrightVit staining for sperm vitality, flourescenin isothiocyanate (FITC)- conjugated peanut agglutinin (PNA) staining for sperm acrosome integrity, induction of hyperactivation by stimulants (sperm preparation media containing capacitating ingredients), and mitochondrial inhibitor (Oligomycin-A) for testing MMP. All functional and structural tests were investigated in both species, except for acrosome integrity, mitochondrial inhibition and functional tests compared over time that could not be successfully completed and investigated in the rhesus species. Motility analysis tests proved that within the vervet species, the use of different physiological media results in statistically significant differences in motility and kinematic parameters over a 1 hour time period. Hyperactivation tests proved that capacitating physiological media produced significantly higher percentages hyperactivation when compared to sperm wash media within the vervet species over a 1 hour time period. Furthermore, within both NHP species, sperm structural analysis (vitality and acrosome integrity) results showed that no significant differences are present when making use of different physiological media over a period of 1 hour incubation. The incubation of vervet sperm with different concentrations of mitochondrial inhibitor, Oligomycin-A (0 μM, 5 μM, and 25 μM), resulted in motility inhibition over a 1 hour incubation period. By the evaluation of these tests it was found that the use of different sperm wash [Human tubal fluid (HTF), Ham‟s F-10® and HD Sperm Wash Plus (HDSWP)] and sperm capacitation media [Human tubal fluid with added caffeine (HTFC) and HD Sperm Capacitating Plus (HDSCP)] resulted in significantly different results within sperm functional tests as compared to sperm structural tests. The study indicates that the composition of media, varying from simple to more complex, used for semen preparation plays an important role in determining NHP sperm functionality. Based on these findings further investigation in larger NHP sample groups and human sperm are required to evaluate the role of certain ingredients in the development of more cost-effective media producing satisfactory results in terms of sperm functionality for artificial reproductive technologies (ART).
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Friedrich, Benjamin. "Chemotaxis of Sperm Cells." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1235056439247-79608.

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Sperm cells are guided to the egg by chemoattractants in many species. Sperm cells are propelled in a liquid by the regular beat of their flagellum. In the presence of a concentration gradient of a chemoattractant, they can steer upwards the concentration gradient, a process called chemotaxis. Eggs release chemoattractants to guide the sperm cells to the egg. Sperm chemotaxis is best studied experimentally in the sea urchin. There, specific receptors in the flagellar membrane of the sperm cells are activated upon binding of chemoattractant molecules and trigger a signaling cascade which ultimately changes the activity of the molecular motors which drive the flagellar beat and result in a swimming response. Sea urchin sperm cells swim along circular and helical paths. Sperm cells of the sea urchin and several other species swim along helical paths far from boundary surfaces in the absence of chemoattractant. In a two-dimensional experimental geometry, sperm swimming paths are planar circles. The non-zero curvature of their swimming paths is a direct consequence of an asymmetry of their flagellar beat. In a concentration gradient of chemoattractant, sperm swimming path are drifting circles in two dimensions and bend helices in three dimensions. What is the working mechanism of sperm chemotaxis? In this thesis, we develop a theoretical description of sperm chemotaxis which can be subsumed as follows: While swimming along an approximately circular path in a concentration gradient a sperm cell traces a periodic concentration stimulus from the concentration field that has the frequency of circular swimming. The chemotactic signaling system processes this stimulus and causes a periodic modulation of the curvature of the swimming path which then gives rise to a swimming path which is a drifting circle. The relative direction of the drift with respect to the gradient direction is determined by the phase shift between the stimulus and the curvature oscillations. This picture is in perfect agreement with recent experimental findings. The mechanism is more general and also works in three dimensions for swimming along helical paths. Our results. Our theoretical description of sperm chemotaxis clarifies the concepts underlying sperm chemotaxis. In particular, we derive the role of internal timing of the chemotactic signaling system for sperm chemotaxis. We conclude that sampling a concentration field along circular and helical paths is a robust strategy for chemotaxis that does not require fine-tuning of parameters and which works reliable also in the presence of fluctuations. In a last chapter of this thesis, we discuss sperm chemotaxis in the more general context of an abstract search problem.
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Friedrich, Benjamin. "Chemotaxis of Sperm Cells." Doctoral thesis, Technische Universität Dresden, 2008. https://tud.qucosa.de/id/qucosa%3A23708.

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Sperm cells are guided to the egg by chemoattractants in many species. Sperm cells are propelled in a liquid by the regular beat of their flagellum. In the presence of a concentration gradient of a chemoattractant, they can steer upwards the concentration gradient, a process called chemotaxis. Eggs release chemoattractants to guide the sperm cells to the egg. Sperm chemotaxis is best studied experimentally in the sea urchin. There, specific receptors in the flagellar membrane of the sperm cells are activated upon binding of chemoattractant molecules and trigger a signaling cascade which ultimately changes the activity of the molecular motors which drive the flagellar beat and result in a swimming response. Sea urchin sperm cells swim along circular and helical paths. Sperm cells of the sea urchin and several other species swim along helical paths far from boundary surfaces in the absence of chemoattractant. In a two-dimensional experimental geometry, sperm swimming paths are planar circles. The non-zero curvature of their swimming paths is a direct consequence of an asymmetry of their flagellar beat. In a concentration gradient of chemoattractant, sperm swimming path are drifting circles in two dimensions and bend helices in three dimensions. What is the working mechanism of sperm chemotaxis? In this thesis, we develop a theoretical description of sperm chemotaxis which can be subsumed as follows: While swimming along an approximately circular path in a concentration gradient a sperm cell traces a periodic concentration stimulus from the concentration field that has the frequency of circular swimming. The chemotactic signaling system processes this stimulus and causes a periodic modulation of the curvature of the swimming path which then gives rise to a swimming path which is a drifting circle. The relative direction of the drift with respect to the gradient direction is determined by the phase shift between the stimulus and the curvature oscillations. This picture is in perfect agreement with recent experimental findings. The mechanism is more general and also works in three dimensions for swimming along helical paths. Our results. Our theoretical description of sperm chemotaxis clarifies the concepts underlying sperm chemotaxis. In particular, we derive the role of internal timing of the chemotactic signaling system for sperm chemotaxis. We conclude that sampling a concentration field along circular and helical paths is a robust strategy for chemotaxis that does not require fine-tuning of parameters and which works reliable also in the presence of fluctuations. In a last chapter of this thesis, we discuss sperm chemotaxis in the more general context of an abstract search problem.
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Svygrys, Tomas. "Šėrimo įtaka veislinių bulių spermos kokybei." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2013. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2013~D_20130618_095738-75852.

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Darbo tikslas: įvertinti skirtingais racionais šertų veislinių bulių spermos kokybę. Darbo uždaviniai: 1. Išnagrinėti ir įvertinti skirtingus veislinių bulių racionus; 2. Nustatyti tiriamųjų bulių spermos kokybę; 3. Įvertinti skirtingų racionų įtaką veislinių bulių spermos kokybiniams rodikliams. Išvados: 1. B fermos buliai reproduktoriai gavo 16,4 MJ AE, 52,35 žaliųjų baltymų, 20,66 g fosforo, 40,9 mg vario, 859,2 mg cinko, 28290 TV vitamino D bei 122700 TV vitamino A daugiau nei A fermos buliai, kur šios medžiagos yra ypač svarbios reprodukcinei sistemai ir spermos kokybei. 2. A fermoje laikomų bulių išskirtas spermos tūris buvo 5,60 (±0,11) ml, koncentracija 1287,61 (±38,04) mln/cm3, gyvybingų spermatozoidų 81,22 (±0,57) proc., tiesiai judančių spermatozoidų 65,73 (±0,76) proc. B fermoje laikomų bulių vidutinis išskiriamas spermos tūris buvo 8,50 (±0,25) ml, koncentracija 1371,54 (±67,07) mln/cm3, gyvybingų spermatozoidų 80,13 (±0,37) proc, tiesiai judančių spermatozoidų 67,81 (±0,47) proc. 3. B fermoje laikomų bulių išskirtas spermos tūris buvo 2,90 ml (P<0,001), koncentracija – 84,92 mln/cm3 (P>0,05), tiesiai judančių spermatozoidų – 2,07 proc. didesnis (P>0,05) nei A fermoje laikomų bulių. Vienintelis priešingai rodęs rodiklis, t.y. gyvybingi spermatozoidai buvo 1,09 proc. (P<0,05) mažesnis už A fermos bulių. 4. Bulių davusių iki 4 ml spermos kiekį, koncentracija vidutiniškai nuo 1351,11(P<0,001) (A buliai) ir 2218,25 (P<0,001) (B buliai) sumažėjo iki 1097,43 (P<0,001)... [toliau žr. visą tekstą]
The aim of this research is evaluate the quality of bull semen, that are fed different diets. Research goal of the paper: 1. Analyze and evaluate different breeding bull rations; 2. Analyze bull’s semen quality; 3. Evaluate the influence of different diets on breeding bull semen quality (semen volume, concentration, percent viable sperm, directly moving spermatozoa percent). The results of different bull’s diet show that bulls of farm B got more mineral and vitamin needed for reproductive system than bulls from farm A. The breeding bulls of farm B were given 16.4 MJ 52.35 crude protein, 20.66 g of phosphorus, 40.9 mg of copper, 859.2 mg of zinc, 28,290 IU of vitamin D and 122 700 IU of vitamin A more than bulls from farm A. This material is particularly important for the reproductive system and sperm quality. Breeding bull’s sperm quality indicators showed that the semen characteristics (volume, concentration, and right-moving sperm) were on average superior to those bulls whose diets were additionally given mineral - vitamin supplements. The semen quality of bulls is changing dependent on different bull semen volume released. It showed that increasing volume of the sperm determine decreasing in sperm concentration mln/cm3.
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Books on the topic "Sperm"

1

Moen, K. Sperm. Victoria, B.C: Hopeace Press, 2006.

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Sperm. Victoria, BC: Hopeace Press, 2005.

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Zini, Armand, and Ashok Agarwal, eds. Sperm Chromatin. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6857-9.

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Pacey, Allan A., and Mathew J. Tomlinson, eds. Sperm Banking. Cambridge: Cambridge University Press, 2009. http://dx.doi.org/10.1017/cbo9781139193771.

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Orlov, Stephen. Sperm count. [Toronto: Playwrights Guild of Canada], 2002.

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Gunderson, Megan M. Sperm whales. Edina, Minn: ABDO Pub. Co., 2011.

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Tsuzuki, Kyoichi. Sperm Palace. Tokyo: Aspect Corp., 2001.

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Sperm whales. Edina, Minn: Abdo & Daughters, 1995.

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Sperm whales. Stillwater, Minn: Voyageur Press, 1998.

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Sperm whales. Grantown-on-Spey: Colin Baxter Photography, 1998.

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Book chapters on the topic "Sperm"

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Sleigh, Merry. "Sperm." In Encyclopedia of Child Behavior and Development, 1427–28. Boston, MA: Springer US, 2011. http://dx.doi.org/10.1007/978-0-387-79061-9_2765.

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Kunz, Yvette W. "Sperm." In Developmental Biology of Teleost Fishes, 131–46. Dordrecht: Springer Netherlands, 2004. http://dx.doi.org/10.1007/978-1-4020-2997-4_8.

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Saucedo, Leslie. "Sperm." In Getting to Know Your Cells, 67–71. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-30146-9_12.

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Oliva, Rafael, and Judit Castillo. "Sperm Nucleoproteins." In Sperm Chromatin, 45–60. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6857-9_3.

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Balhorn, Rod. "Sperm Chromatin: An Overview." In Sperm Chromatin, 3–18. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6857-9_1.

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Gosálvez, Jaime, Carmen López-Fernández, and José Luís Fernández. "Sperm Chromatin Dispersion Test: Technical Aspects and Clinical Applications." In Sperm Chromatin, 151–70. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6857-9_10.

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Manicardi, Gian Carlo, Davide Bizzaro, and Denny Sakkas. "Basic and Clinical Aspects of Sperm Chromomycin A3 Assay." In Sperm Chromatin, 171–79. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6857-9_11.

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Tsarev, Igor, and Juris Erenpreiss. "Cytochemical Tests for Sperm Chromatin Maturity." In Sperm Chromatin, 181–88. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6857-9_12.

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Varghese, Alex C., C. Fischer-Hammadeh, and M. E. Hammadeh. "Acridine Orange Test for Assessment of Human Sperm DNA Integrity." In Sperm Chromatin, 189–99. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6857-9_13.

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Sharma, Rakesh, and Ashok Agarwal. "Laboratory Evaluation of Sperm Chromatin: TUNEL Assay." In Sperm Chromatin, 201–15. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6857-9_14.

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Conference papers on the topic "Sperm"

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Xue, Baigong, Zhanfei Yao, Yeling Wang, and Lianwen Zheng. "Assayed Sperm Damnification by SCGE and Its Correlation with Sperm Motility and Sperm Malformation Rate." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780103.

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Salari, Valiollah. "Sperm motion analysis." In Electronic Imaging '91, San Jose,CA, edited by Alan C. Bovik and Vyvyan Howard. SPIE, 1991. http://dx.doi.org/10.1117/12.44301.

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Wang, Yongxin, Yanping Jia, Ming Yuchi, and Mingyue Ding. "The Computer-Assisted Sperm Analysis (CASA) Technique for Sperm Morphology Evaluation." In 2011 International Conference on Intelligent Computation and Bio-Medical Instrumentation (ICBMI). IEEE, 2011. http://dx.doi.org/10.1109/icbmi.2011.21.

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Osypchuk, G., Nina Bradu, Irina Gengera, Ion Balan, and S. Povetkin. "К вопросу влияния биологически активных веществ (БАВ) на сперматогенез и некоторые биохимические показатели крови." In Scientific and practical conference with international participation: "Management of the genetic fund of animals – problems, solutions, outlooks". Scientific Practical Institute of Biotechnologies in Animal Husbandry and Veterinary Medicine, 2023. http://dx.doi.org/10.61562/mgfa2023.40.

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The use of biologically active substances allows you to activate the functions of all organs and systems of the body. The aim of the research was to study the effect of BAS agents on the qualitative and quantitative parameters of sperm and some biochemical parameters of the blood of boars and rams. During the research, it was found out that the non-hormonal BAS agents used by us can improve spermatogenesis, do not have a negative effect on the body, stimulate metabolic processes. It was found that the qualitative and quantitative parameters of the seed (the number of live sperms and the number of rectilinearly translational sperms) increase more intensively in animals of the experimental groups.
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Oberloskamp, Helga. "Sperm Donation and Adoption." In 26th Conference Medicine, Law & Society. University of Maribor Press, 2017. http://dx.doi.org/10.18690/978-961-286-021-9.14.

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Magdanz, Veronika, Johannes Gebauer, Dalia Mahdy, Juliane Simmchen, and Islam S. M. Khalil. "Sperm-templated magnetic microrobots." In 2019 International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS). IEEE, 2019. http://dx.doi.org/10.1109/marss.2019.8860953.

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Hidayatullah, Priyanto, Iwan Awaludin, Reyhan Damar Kusumo, and Muhammad Nuriyadi. "Automatic sperm motility measurement." In 2015 International Conference on Information Technology Systems and Innovation (ICITSI). IEEE, 2015. http://dx.doi.org/10.1109/icitsi.2015.7437674.

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Fujii, Takuro, Hayato Nakagawa, Teppei Takeshima, Yasushi Yumura, and Tomoki Hamagami. "Automated Sperm Assessment Framework and Neural Network Specialized for Sperm Video Recognition." In 2024 IEEE/CVF Winter Conference on Applications of Computer Vision (WACV). IEEE, 2024. http://dx.doi.org/10.1109/wacv57701.2024.00750.

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Park, Daniel S., Robert Egnatchik, Hali Bordelon, Terrence R. Tiersch, and W. Todd Monroe. "A Microfluidic Mixer to Activate Sperm Cells of Aquatic Species for Standardization of Computer-Assisted Motion Analysis." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53839.

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The objective of this paper is to develop a microfluidic device to: 1) activate a small volume of aquatic species sperm by rapid mixing with diluent, and 2) position sperm in a viewing chamber for motility evaluation using computer-assisted sperm analysis (CASA). Analysis of aquatic species sperm is becoming more important as the use of fish as biomedical models expands. Because it is more efficient to maintain frozen stocks of genetic material rather than thousands of research lines of adult fish, there has been increased study on cryopreservation for model fish. The analysis of fish gametes is challenging due to small sample size, short motility duration, and inconsistent activation (motility induction). For many aquatic species, sperm motility is initiated through the manual alteration of the medium osmolality, typically accomplished through manual dilution and mixing by hand. Manual methods limit control over the activation process and therefore viability analysis. The short lifespan of these cells makes CASA challenging due to the limitations in capturing and processing data rapidly enough to monitor the peak motility, as CASA systems were designed for mammalian sperm which have a longer motility duration.
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Bouloorchi, Mohammadjavad, Saeed Javadizadeh, Aref Valipour, MirBehrad Mousavi, and Majid Badieirostami. "Numerical Study of a Microfluidic-Based Motile Sperm Enrichment Using Sperm Rheotaxis Behavior." In 2023 31st International Conference on Electrical Engineering (ICEE). IEEE, 2023. http://dx.doi.org/10.1109/icee59167.2023.10334731.

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Reports on the topic "Sperm"

1

Golovsky, David, and Shannon Kim. Sperm retrieval for assisted reproduction. BJUI Knowledge, November 2020. http://dx.doi.org/10.18591/bjuik.0528.

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Golovsky, David, and Shannon Kim. Sperm retrieval for assisted reproduction. BJUI Knowledge, November 2020. http://dx.doi.org/10.18591/bjuik.0528.v2.

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Bodt, B. A., and R. J. Young. Hyperactivated Rabbit Sperm Cell Motility Parameters. Fort Belvoir, VA: Defense Technical Information Center, March 1995. http://dx.doi.org/10.21236/ada294502.

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Kailash, Yamini, and Tet Yap. Klinefelter syndrome and success of sperm retrieval. BJUI Knowledge, November 2020. http://dx.doi.org/10.18591/bjuik.0531.v2.

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Smith, Ian. Hey Dad! How sperm donors lost their anonymity. Edited by Grace Jennings-Edquist. Monash University, November 2023. http://dx.doi.org/10.54377/404f-ce4a.

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Tsvetkov, Tvetan, and Denica Daskalova. Analysis of Seminal Plasma Proteins Related to Sperm Hyperactivation. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, January 2021. http://dx.doi.org/10.7546/crabs.2021.01.09.

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Watkins, William A. Tag Development and Response of Sperm Whales to Low Frequencies. Fort Belvoir, VA: Defense Technical Information Center, June 1994. http://dx.doi.org/10.21236/ada282974.

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Watkins, William A. Tag Development and Response of Sperm Whales to Low Frequencies. Fort Belvoir, VA: Defense Technical Information Center, June 1994. http://dx.doi.org/10.21236/ada283383.

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Young, Ronald J., and David Burnett. Chemical Inhibition of Rabbit Sperm Cell Motility in Toxicological Testing. Fort Belvoir, VA: Defense Technical Information Center, January 1990. http://dx.doi.org/10.21236/ada219488.

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Prince, R. M. Sperm cells as vectors in the production of transgenic animals. Office of Scientific and Technical Information (OSTI), April 1993. http://dx.doi.org/10.2172/10175384.

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You might also be interested in the extended bibliographies on the topic 'Sperm' for particular source types:
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