Journal articles: 'Spectrophotometer analysi'

1

Shehata, Adel B., Abdulrahman R. Alaskar, Mohammed A. Alrasheed, Abdullah S. Alosaimi, Fahd A. Alkharraa, and Abdulrahman M. Alzahrani. "Certification of sodium benzoate solution reference material by HPLC-UV, LC-MS/MS and UV-VIS-NIR spectrophotometry for food and drug analysi." Journal of Chemical Metrology 14, no. 2 (November 28, 2020): 88–105. http://dx.doi.org/10.25135/jcm.48.20.08.1780.

Full text

Abstract:

Sodium benzoate is one of the most widely used food perseverates and has to be used in regulated amounts to avoid its harmful health effects. Reviewing the scientific literature for traceability of the analytical measurement results of sodium benzoate in various food and drug applications to the SI units, it has been found that no scientific details of certification of sodium benzoate CRMs used for the calibration of measuring equipment are published. For this reason, the national metrology institute of Saudi Arabia (SASO/NMCC) certifies a sodium benzoate solution reference material. In this work, sodium benzoate was synthesized, purified and a batch solution reference material was prepared as 1014.47 mg/kg then homogenized and bottled. Homogeneity and stability of the candidate RM were assessed and the results obtained showed that the material is sufficiently homogeneous and stable. Characterization of the reference material was carried out by HPLC-UV, LC-MS/MS and UV-VIS-NIR spectrophotometer as three independent methods. The certified value was derived by combining data from the three methods using the weighted mean approach and was found 1016.13 mg/kg. The certified uncertainty was calculated as weighted uncertainty and was found 10.47 mg/kg (1.03%). Sources of this uncertainty were identified from the characterization, uchar, homogeneity, uhom, and long-term stability, ults, as well as the bias allowance, B.

APA, Harvard, Vancouver, ISO, and other styles

2

Mohammad, Karim Aly, Abdelhalim Zekry, and Mohamed Abouelatta. "LED Based Spectrophotometer can compete with conventional one." International Journal of Engineering & Technology 4, no. 2 (May 24, 2015): 399. http://dx.doi.org/10.14419/ijet.v4i2.4504.

Full text

Abstract:

Spectrophotometers are the common devise used for blood analysis. These analyses are simply optioned from the transmittance of monochromatic light through a blood sample. It disperses their light sources by different techniques; the simplest one is the use of interference filters in front of broad-band light source. As Interference bandpass filters are relatively inexpensive wavelength selectors. Interference filters that allow transmission of a predetermined wavelength while rejecting or blocking other wavelengths are widely used in instrumentation for clinical chemistry. On the other hand, the lights emitting diodes (LEDs) emits a specified band and have a wide selection options. An economic device based on LEDs (LED based spectrometer) is explained in this work. Instead of the wide spectrum light source and filters (commercial spectrophotometer), The LED based spectrophotometer is calibrated with standard solutions, and its measurement results is compared with the Mindray BS-200 Chemistry Analyzer (commercial spectrophotometer). The comparison shows acceptable results between both spectrophotometers with maximum error 2.33%. The errors can be reduced by applying more fining alignment. Another benefit of the new designee is to minimize testing costs.

APA, Harvard, Vancouver, ISO, and other styles

3

Dhawale, Nandkishor M., Viacheslav I. Adamchuk, Shiv O. Prasher, Raphael A. Viscarra Rossel, and Ashraf A. Ismail. "Evaluation of Two Portable Hyperspectral-Sensor-Based Instruments to Predict Key Soil Properties in Canadian Soils." Sensors 22, no. 7 (March 26, 2022): 2556. http://dx.doi.org/10.3390/s22072556.

Full text

Abstract:

In contrast with classic bench-top hyperspectral (multispectral)-sensor-based instruments (spectrophotometers), the portable ones are rugged, relatively inexpensive, and simple to use; therefore, they are suitable for field implementation to more closely examine various soil properties on the spot. The purpose of this study was to evaluate two portable spectrophotometers to predict key soil properties such as texture and soil organic carbon (SOC) in 282 soil samples collected from proportional fields in four Canadian provinces. Of the two instruments, one was the first of its kind (prototype) and was a mid-infrared (mid-IR) spectrophotometer operating between ~5500 and ~11,000 nm. The other instrument was a readily available dual-type spectrophotometer having a spectral range in both visible (vis) and near-infrared (NIR) regions with wavelengths ranging between ~400 and ~2220 nm. A large number of soil samples (n = 282) were used to represent a wide variety of soil textures, from clay loam to sandy soils, with a considerable range of SOC. These samples were subjected to routine laboratory soil analysis before both spectrophotometers were used to collect diffuse reflectance spectroscopy (DRS) measurements. After data collection, the mid-IR and vis-NIR spectra were randomly divided into calibration (70%) and validation (30%) sets. Partial least squares regression (PLSR) was used with leave one out cross-validation techniques to derive the spectral calibrations to predict SOC, sand, and clay content. The performances of the calibration models were reevaluated on the validation set. It was found that sand content can be predicted more accurately using the portable mid-IR spectrophotometer and clay content is better predicted using the readily available dual-type vis-NIR spectrophotometer. The coefficients of determination (R2) and root mean squared error (RMSE) were determined to be most favorable for clay (0.82 and 78 g kg−1) and sand (0.82 and 103 g kg−1), respectively. The ability to predict SOC content precisely was not particularly good for the dataset of soils used in this study with an R2 and RMSE of 0.54 and 4.1 g kg−1. The tested method demonstrated that both portable mid-IR and vis-NIR spectrophotometers were comparable in predicting soil texture on a large soil dataset collected from agricultural fields in four Canadian provinces.

APA, Harvard, Vancouver, ISO, and other styles

4

Morawski, Roman Z. "Measurement Data Processing in Spectrophotometric Analysers of Food." Metrology and Measurement Systems 19, no. 4 (December 1, 2012): 623–52. http://dx.doi.org/10.2478/v10178-012-0056-1.

Full text

Abstract:

Abstract Spectrometry, especially spectrophotometry, is getting more and more often the method of choice not only in laboratory analysis of (bio)chemical substances, but also in the off-laboratory identification and testing of physical properties of various products, in particular - of various organic mixtures including food products and ingredients. Specialised spectrophotometers, called spectrophotometric analysers, are designed for such applications. This paper is on the state of the art in the domain of data processing in spectrophotometric analysers of food (including beverages). The following issues are covered: methodological background of food analysis, physical and metrological principles of spectrophotometry, the role of measurement data processing in spectrophotometry. General considerations are illustrated with examples, predominantly related to wine and olive oil analysis.

APA, Harvard, Vancouver, ISO, and other styles

5

Kholifa, Mulik, Tri Mulyono, and Yeni Maulidah Muflihah. "Simultaneous Determination of Magnesium (Mg2+) and Ammonium (NH4+) by Flow Injection Analysis." Jurnal ILMU DASAR 19, no. 1 (February 28, 2018): 1. http://dx.doi.org/10.19184/jid.v19i1.5483.

Full text

Abstract:

An automatic method for the simultaneous determination of magnesium and ammoniun by flow injection analysis is described. This analysis used two detectors, potentiometry and spectrophotometry, for the determination of magnesium and ammonium simultaneously. Potentiometric detectors are used to detect ammonium ions, whereas spectrophotometer detectors are used to detect the presence of magnesium ions. The performance of the FIA system includes linear range linearity, limit of detection, sensitivity, reproducibility and recovery test. Spectrophotometry FIA showed good performance with several criteria of linearity with regression value of 0.9918, the detection limit of 0.06 ppm, a sensitivity of 0.0115, repeatability of less than 2% and the percent recovery of 91,6%. While potentiometric FIA showed th performance with several criteria of linearity with regression value of 0.9896, the detection limit of 0.17 ppm, a sensitivity of 40.747 mV per decade of concentration, repeatability of less than 2% and the percent recovery of 89,7%. Keywords: Flow Injection Analysis, spectrophotometry, potensiometry, simultaneous

APA, Harvard, Vancouver, ISO, and other styles

6

Mouazen, A. M., W. Saeys, J. Xing, J. De Baerdemaeker, and H. Ramon. "Near Infrared Spectroscopy for Agricultural Materials: An Instrument Comparison." Journal of Near Infrared Spectroscopy 13, no. 2 (April 2005): 87–97. http://dx.doi.org/10.1255/jnirs.461.

Full text

Abstract:

The selection of a spectrophotometer for the measurement of constituents of agricultural materials with acceptable accuracy and cost effectiveness requires a comparative study of the performance of different spectrophotometers. Four commercially available spectrophotometers were evaluated, based on measurements performed on three agricultural materials. These spectrophotometers, differing mainly in wavelength range and measurement principles, comprised a diode array (DA) of 300–1700 nm, a combination of diode array and scanning monochromator (DASM) of 350–2500 nm, a Fourier transform (FT) of 750–2500 nm and a scanning monochromator (SM) of 400–2500 nm spectrophotometers. They were used to measure the moisture content of soil, the chemical constituents of hog manure and to detect bruising in apples. Three spectral pre-treatments were considered. Calibrations were developed using partial least squares (PLS) regression with the leave-one-out cross-validation technique for soil and manure and principal component analysis (PCA) for apple. The four instruments provided good predictions for soil moisture content, with the largest coefficient of determination ( r2) values between 0.84–0.86 and with the largest ratio of prediction to deviation ( RPD) of standard deviation ( SD) to root mean square error of cross-validation ( RMSECV) ranging from 2.53 to 2.75. The DASM and SM were comparable and slightly better than the DA and FT. For hog manure, total nitrogen was predicted more accurately with the four instruments ( r2 = 0.83–0.89 and RPD = 2.43–3.01) than the phosphorus ( r2 = 0.66–0.85 and RPD = 1.72–2.61) and potassium ( r2 = 0.70–0.84 and RPD = 1.83–2.50). The order of accuracy of the four spectrophotometers for the measurement of total nitrogen was: FT–SM–DA–DASM. For phosphorus and potassium the order was: DA–DASM–FT–SM and SM–FT–DASM–DA, respectively. The DA performed better than the DASM and FT for discrimination of bi-colour and single-colour bruised apple, respectively. Therefore, selection of a spectrophotometer depends mainly on the type of material analysed and the constituent to be measured. Wavelengths above 1700 nm were found unnecessary for the applications considered and the DA spectrophotometer was of sufficient accuracy, as it is robust, significantly cheaper and can be used in the field for on-line measurements.

APA, Harvard, Vancouver, ISO, and other styles

7

Saputro, Sulistyo, Ashadi Ashadi, Lina Mahardiani, Nurma Yunita Indriyanti, Maria Ciptaning Sabdo Kawedhar, and Wima Pudya Ajunda. "The Analysis of Low-Cost Pb(II) Adsorbents using Batch Method of Solid-Phase Spectrophotometry." Jurnal Kimia Valensi 7, no. 1 (June 21, 2021): 38–45. http://dx.doi.org/10.15408/jkv.v7i1.18363.

Full text

Abstract:

Heavy metal pollutants contained in wastewater can cause health problems for living things around. Minor to fatal health problems can occur due to heavy metal poisoning, mainly caused by Pb(II) metal.. This study aimed to determine the optimum mass combination of rice husk and zeolite to adsorb Pb(II) metal ions in simulated wastewater, and to determine the sensitivity of the analysis method. This study used Solid Phase Spectrophotometry (SPS) to determine the decrease in Pb(II) metal ion levels after being adsorbed by activated carbon from rice husks and zeolites. This study used an experimental method with simulated wastewater samples containing Pb(II) at several concentrations. Pb(II) adsorption processes by rice husk and natural zeolite used various adsorbents' mass ratios. The adsorbents were characterized by using Fourier-Transform Infra-Red (FTIR) Spectrophotometry. Pb(II) analysis during adsorption processes used a single beam UV-visible Spectrophotometer for Solid-Phase Spectrophotometry. This study indicates that the combination of adsorbent from rice husk and natural zeolite can properly adsorb Pb(II) ions with an adsorption capacity of 0.75 μg g-1 and 0.025 μg L-1 for the LoD of the instrument.

APA, Harvard, Vancouver, ISO, and other styles

8

Saputro, Sulistyo, Ashadi Ashadi, Lina Mahardiani, Nurma Yunita Indriyanti, Maria Ciptaning Sabdo Kawedhar, and Wima Pudya Ajunda. "The Analysis of Low-Cost Pb(II) Adsorbents using Batch Method of Solid-Phase Spectrophotometry." Jurnal Kimia Valensi 1, no. 1 (June 21, 2021): 38–45. http://dx.doi.org/10.15408/jkv.v1i1.18363.

Full text

Abstract:

Heavy metal pollutants contained in wastewater can cause health problems for living things around. Minor to fatal health problems can occur due to heavy metal poisoning, mainly caused by Pb(II) metal.. This study aimed to determine the optimum mass combination of rice husk and zeolite to adsorb Pb(II) metal ions in simulated wastewater, and to determine the sensitivity of the analysis method. This study used Solid Phase Spectrophotometry (SPS) to determine the decrease in Pb(II) metal ion levels after being adsorbed by activated carbon from rice husks and zeolites. This study used an experimental method with simulated wastewater samples containing Pb(II) at several concentrations. Pb(II) adsorption processes by rice husk and natural zeolite used various adsorbents' mass ratios. The adsorbents were characterized by using Fourier-Transform Infra-Red (FTIR) Spectrophotometry. Pb(II) analysis during adsorption processes used a single beam UV-visible Spectrophotometer for Solid-Phase Spectrophotometry. This study indicates that the combination of adsorbent from rice husk and natural zeolite can properly adsorb Pb(II) ions with an adsorption capacity of 0.75 μg g-1 and 0.025 μg L-1 for the LoD of the instrument.

APA, Harvard, Vancouver, ISO, and other styles

9

Darmawati, Darmawati, Syaiful Bahri, and Husain Sosidi. "Analisis Kadar Glukomanan Dari Biji Durian (Durio zeibethinus Murr) dengan Metode Spektrofotometri pada Berbagai Waktu dan Suhu Hidrolisis." KOVALEN: Jurnal Riset Kimia 6, no. 2 (September 1, 2020): 158–64. http://dx.doi.org/10.22487/kovalen.2020.v6.i2.11582.

Full text

Abstract:

Durian seeds are one type of seed that contains glucomannan. The aim is to determine glucomannan levels in the durian seed. The study was designed using a completely randomized design (CRD). The extracted glucomannan was determined by the spectrophotometry method at various temperature and hydrolysis time. The crude extract of glucomannan was hydrolyzed using 3 M HCl for 70, 80, 90, 100, and 110 minutes at 70, 80, 90, 100, and 110°C. The highest glucomannan concentration of 39.60% was obtained at a hydrolysis temperature of 100°C for 100 minutes. The analysis of the FTIR spectrophotometer shown that the vibration of the C-O-C functional group (glycoside bond) was obtained at the wavelength number of 1020.34 cm-1 which is a characteristic of glucomannan. Keywords: glucomannan, durian seed, hydrolysis, spectrophotometry

APA, Harvard, Vancouver, ISO, and other styles

10

Brescia, P. "Multi-Volume Analysis of Nucleic Acids Using the EPOCH™ Spectrophotometer System." Nauka ta innovacii 8, no. 2 (March 30, 2012): 43–47. http://dx.doi.org/10.15407/scin8.02.043.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Andulaai, Arlina Mayharty, Ruslan Ruslan, Hardi Ys., and Dwi Juli Puspitasari. "STUDI PERBANDINGAN ANALISIS VITAMIN E MINYAK SAWIT MERAH TERSAPONIFIKASI ANTARA METODE SPEKTROFOTOMETRI UV-VIS DAN KCKT." KOVALEN 3, no. 1 (April 30, 2017): 50. http://dx.doi.org/10.22487/j24775398.2017.v3.i1.8233.

Full text

Abstract:

A research about a comparative study of spectrophotometry UV-Vis and HPLC method for the analysis of vitamin E in saponified red palm oil has been done. This research aims to compare the results of analysis using Spectrophotometer UV - Vis and HPLC to determine the concentration of vitamin E in red palm oil previously saponified and extracted. HPLC analysis was carried out using an RP-18 column and mobile phase composed a methanol and water ( 86:14 ), with a flow rate of 1 ml/min and UV detection at 290 nm. For the Spectrophotometric UV-Vis analysis, hexane was used as a solvent and the wavelength at 298,5 nm was selected for the detection. The results are the concentration of vitamin E using spectrophotometric and HPLC method was respectively 104.5 ppm and 127 ppm.Keyword: Vitamin E, Red Palm Oil, saponification, extraction, spectrophotometry Ultra Violet -Visible, High Performance Liquid Chromatography

APA, Harvard, Vancouver, ISO, and other styles

12

Gomes, Patrícia, Carla M. U. Negretto, Zanandria B. Naisinger, Ricardo Lorenzoni, Nathalie Ribeiro Wingert, and Renata P. Raffin. "Second-derivative spectrophotometry for the analysis of simvastatin in polymeric nanocapsules." Drug Analytical Research 3, no. 2 (December 23, 2019): 12–17. http://dx.doi.org/10.22456/2527-2616.98057.

Full text

Abstract:

Conventional spectrophotometry methods are very susceptible to the presence of interferences in complex mixtures such as nanoparticules, requiring prior treatment or extraction of the analyte, and not always providing an adequate response. Derivative spectrophotometry method is capable to eliminate its interference; it is an alternative method for drugs determination in complex matrices. This work investigated the utility of derivate spectrophotometry in assay of simvastatin in polymeric nanocapsules (SIVNC). Shimadzu® UV-1650 double-beam spectrophotometer with 1.0 cm quartz cells was used in this study. The second-order derivative spectrum was obtained employing Δλ=20,000 nm and scaling factor=9.0. The determinations were made at 239 nm (2D239) by zero-crossing method. 2D239 method was validated employing the parameters: specificity, linearity, robustness, precision and accuracy. Results: The specificity test showed there was no interference of constituents commonly found in SIVNC formulation in 2D239. The standard curve showed a correlation coefficient of 0.994. The robustness was evaluated by small changes in the conditions of sample analysis and however, no significant changes were observed regarding drug quantitation. The precision was demonstrated by relative standard deviation (RSD) of intra-day (RSD=1.61-3.76) and inter-day studies (RSD=2.32). The recovery test resulted in an average of 100.66%, which confirmed the accuracy of the method. The procedure was simple and rapid; therefore this technique offers an alternative for determination of SIVNC without interferences.

APA, Harvard, Vancouver, ISO, and other styles

13

Welleweerd, Marcel K., Tijmen Hageman, Marc Pichel, Dave van As, Hans Keizer, Jordi Hendrix, Mina M. Micheal, et al. "An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles." Review of Scientific Instruments 93, no. 9 (September 1, 2022): 094101. http://dx.doi.org/10.1063/5.0098008.

Full text

Abstract:

We present a spectrophotometer (optical density meter) combined with electromagnets dedicated to the analysis of suspensions of magnetotactic bacteria. The instrument can also be applied to suspensions of other magnetic cells and magnetic particles. We have ensured that our system, called MagOD, can be easily reproduced by providing the source of the 3D prints for the housing, electronic designs, circuit board layouts, and microcontroller software. We compare the performance of our system to existing adapted commercial spectrophotometers. In addition, we demonstrate its use by analyzing the absorbance of magnetotactic bacteria as a function of their orientation with respect to the light path and their speed of reorientation after the field has been rotated by 90°. We continuously monitored the development of a culture of magnetotactic bacteria over a period of 5 days and measured the development of their velocity distribution over a period of one hour. Even though this dedicated spectrophotometer is relatively simple to construct and cost-effective, a range of magnetic field-dependent parameters can be extracted from suspensions of magnetotactic bacteria. Therefore, this instrument will help the magnetotactic research community to understand and apply this intriguing micro-organism.

APA, Harvard, Vancouver, ISO, and other styles

14

Azis, Sayed Abdul, Muhammad Irham, Sugianto Sugianto, Ichsan Setiawan, and Amri Adnan. "Analysis of research methodology on the content of heavy metals in sediments on the Indonesian coastal." Depik 10, no. 3 (December 30, 2021): 260–66. http://dx.doi.org/10.13170/depik.10.3.21275.

Full text

Abstract:

Heavy metal pollution is one of the problems that often occurs in Indonesian coastal waters. Comparative methods of looking at heavy metal content in coastal sediments include a way to show similarities and differences between one or more articles by using certain criteria. Different comparison methods to see the heavy metal content in sediments include the AAS (Atomic Absorption Spectrophotometer) method, AAN (Netron Activation Analysis), Regression, and USEPA methods. Based on the comparison of 2 methods, namely: AAS and AAN methods, the most efficient in analyzing heavy metals in sediments in coastal areas is the Atomic Absorption Spectrophotometry (AAS) method compared to the Neutron Activation Analysis (AAS) method.Keywords:CoastalSedimentHeavy MetalSpectrophotometer

APA, Harvard, Vancouver, ISO, and other styles

15

Pratama, Mamat, Raiz Razak, and Vivien Sandra Rosalina. "ANALISIS KADAR TANIN TOTAL EKSTRAK ETANOL BUNGA CENGKEH (Syzygium aromaticum L.) MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS." Jurnal Fitofarmaka Indonesia 6, no. 2 (August 7, 2019): 368–73. http://dx.doi.org/10.33096/jffi.v6i2.510.

Full text

Abstract:

Clove flower (Syzygium aromaticum L.) contain saponins, tannins, alkaloids, glycosides and flavonoids. The research aimed to qualitatively and quantitatively determine the existence of tannins in ethanol extract of clove flower by UV-Vis Spectrophotometry. The extraction process used was maceration using ethanol of 30% with the yield value of 4.26%. The extract obtained was measured by UV-Vis Spectrophotometer at the maximum wavelength of 649.9 nm using tanic acid as the standard. The value of the measurement result of tannin content obtained in the ethanol extract of clove flower was 300.826 mg TAE/g extract or 30.0826% b/b TAE.

APA, Harvard, Vancouver, ISO, and other styles

16

Blanco, Marcelo, Jordi Coello, Hortensia Iturriaga, Santiago Maspoch, and Esther Rovira. "Wavelength Calibration Transfer between Diode Array UV-Visible Spectrophotometers." Applied Spectroscopy 49, no. 5 (May 1995): 593–97. http://dx.doi.org/10.1366/0003702953964084.

Full text

Abstract:

The need to obtain expeditious results in control analyses of complex mixtures has turned multivariate calibration procedures into major choices for routine analyses. The inherent complexity of the calibration process and the practical need for analyses to be carried out as near the manufacturing line as possible occasionally entail calibrating with a different instrument from that subsequently employed for the analytical measurements proper. This paper exposes the problems potentially arising in transferring calibrations between diode array UV-Vis spectrophotometers. Basically, such problems originate in wavelength differences between spectrophotometers, even if they meet the manufacturer's specifications and the pharmacopoeia recommendations. We developed a straightforward method for harmonizing instrumental responses on the basis of reference wavelengths corresponding to zero values in the first-derivative spectra for potassium dichromate and benzoic acid standards. The method was applied to the analysis of binary mixtures of theophylline and doxylamine by multiple linear and partial least-squares regression with the use of one spectrophotometer for calibration and four others for analyses.

APA, Harvard, Vancouver, ISO, and other styles

17

Tam, K. Y., and F. T. Chau. "Simultaneous multiwavelength study of the reaction of phenolphthalein with sodium hydroxide." Journal of Automatic Chemistry 14, no. 5 (1992): 157–62. http://dx.doi.org/10.1155/s1463924692000294.

Full text

Abstract:

A photodiode array (PDA) spectrophotometer was used to study the fading reaction of phenolpthalein in dilute sodium hydroxide solution. The principal component analysis (PCA) method was employed to identify the number of light absorbing species in the kinetics system. The target factor analysis (TFA) procedure, coupled with the Broyden-Fletcher-Goldfard-Shanno (BFGS) optimization method, was applied to the observed data to deduce the rate constants and the concentration-time profile of the reaction. The internal referencing method was shown to be essential in improving the quality of data obtained by a single beam PDA spectrophotomer.

APA, Harvard, Vancouver, ISO, and other styles

18

Dupuit, E., A. Dandrieux, P. Kvapil, J. Ollivier, G. Dusserre, and O. Thomas. "UV spectrophotometry for monitoring toxic gases." Analusis 28, no. 10 (December 2000): 966–72. http://dx.doi.org/10.1051/analusis:2000163.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

YANTI, IKA. "Determination of Cr (VI) and SO42- Using UV-Vis Spectrophotometry in River Water Samples at the Environment Office of Semarang City." INDONESIAN JOURNAL OF CHEMICAL RESEARCH 6, no. 2 (January 17, 2022): 51–58. http://dx.doi.org/10.20885/ijcr.vol6.iss2.art1.

Full text

Abstract:

Analysis of hexavalent chromium (Cr-VI) and sulfate ions (SO42-) in river water in the city of Semarang aims to find out the quality of river water compared to Government Regulation No. 22 of 2021. Concentration of chromium (Cr-VI) were determined using the spectrophotometry method with 1,5-diphenylcarbazide reagent and analyzed with a UV-Vis spectrophotometer at a wavelength of 540 nm, while the determination of sulfate levels (SO42-) using the turbidimetric method uses the same spectrophotometer at a wavelength of 420 nm. The results of the analysis obtained Cr(VI) of 0.0094 mg/L and 0.0096 mg/L while SO42- amounted to 17.2105 mg/L and 20.6842 mg/L. The results showed that river water based on PP Number 22 of 2021 on class II river water quality standards, river water quality is still good and can be used according to its designation because it is still below the maximum threshold of Cr(VI) and SO42- which is 0.05 mg/L for Cr (VI) and 300 mg/L levels for SO42-levels.

APA, Harvard, Vancouver, ISO, and other styles

20

E.B. da Silva, Fabiana, Willian R.R. Almeida, Fávero R. Paula, Aline L.H. Müller, Érico M.M. Flores, and Marco F Ferrão. "The effect of pH on the simultaneous determination of Sulfamethoxazole and Trimethoprim by Ultraviolet Spectrophotometry and Multivariate Calibration." Drug Analytical Research 1, no. 2 (December 28, 2017): 15–23. http://dx.doi.org/10.22456/2527-2616.79218.

Full text

Abstract:

Analytical techniques based on Ultraviolet (UV) spectrophotometry are widely used in pharmaceutical analysis, because they are simple and inexpensive. The choice of pH is critical in the development of univariate methods for pharmaceutical quantitation by UV spectrophotometry since changes may modify the absorption spectrum profile. Similar to univariate methods by UV spectrophotometry changes in pH may influence the predictive ability of multivariate models, affecting the resultant analytical performance. We report herein on the influence of pH on the simultaneous determination of sulfamethoxazole (SMZ) and trimethoprim (TMP) in tablets using UV spectrophotometry and multivariate calibration. Data were recorded using a UV spectrophotometer in the wavelength range of 200 to 350 nm. The experimental matrix was constructed using 36 synthetic samples of SMZ-TMP mixtures. The concentration ranges used for the investigation were 14.0 to 26.0 mg L-1 for SMZ and 2.8 to 5.2 mg L-1 for TMP. The Partial Least Squares (PLS) regression models were generated with full-spectrum and multiple pH levels. At pH 4.3, lower values of relative standard error of prediction (RSEP %) for SMZ (1.83) and TMP (1.13) were obtained. The PLS model at pH 4.3 was used for the quantification of real samples (tablets obtained from 13 different manufacturers) and the results were compared with conventional procedures using high performance liquid chromatography (HPLC).

APA, Harvard, Vancouver, ISO, and other styles

21

YAMADA, Tomoko, Atsush YAMAMOTO, Akinobu MATSUNAGA, Takaho WATANABE, and Hiroyuki NAKAZAWA. "An Alternative Method for CD Measurement by a Conventional Spectrophotometer." Analytical Sciences 16, no. 3 (2000): 325–27. http://dx.doi.org/10.2116/analsci.16.325.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Kozioł, Michał, Daria Wotzka, Tomasz Boczar, and Paweł Frącz. "Application of Optical Spectrophotometry for Analysis of Radiation Spectrum Emitted by Electric Arc in the Air." Journal of Spectroscopy 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/1814754.

Full text

Abstract:

This paper presents the results of measurement and analysis of optical radiation emitted by a free burning electric arc. The aim was to determine the application possibilities of optical spectrophotometry for detection of electric arcs. The research works considered electric arc generated with a constant voltage supply between two copper electrodes in the air, carried out under laboratory conditions. A high resolution optical spectrophotometer was used for registration of optical radiation. The analyses involved determination of two dimensionless descriptors obtained for the gathered spectra. Moreover, for each of the registered intensity distributions, the energy values were calculated for three frequency ranges. Based on the measured signals, the possibility of application of spectrophotometry for the optical radiation analysis was confirmed. The analysis indicated that the most energy of optical radiation is detected for the range of 200–780 nm, while above 780 nm almost no optical energy is emitted. Spectrophotometric studies performed in the UV-NIR range are of interest since one can obtain information about the structural defects (at lower wavebands) or impurities and/or point defects (at low energies bands). It was also stated that the obtained descriptors may be applied for diagnosis and identification of electric arc purposes.

APA, Harvard, Vancouver, ISO, and other styles

23

Payne, Gemma, Neil Langlois, Chris Lennard, and Claude Roux. "Applying visible hyperspectral (chemical) imaging to estimate the age of bruises." Medicine, Science and the Law 47, no. 3 (July 2007): 225–32. http://dx.doi.org/10.1258/rsmmsl.47.3.225.

Full text

Abstract:

Hyperspectral (chemical) imaging collects spectroscopic data in a two-dimensional spatial format. The potential application for the determination of the age of bruises is demonstrated and compared to reflectance probe spectrophotometry as well as photography. Blood was deposited on white cotton cloth or injected subcutaneously into pig skin to simulate a ‘fresh bruise’. A mixture of blood and bile was used to simulate ‘old’ bruises. On the cloth background all the photographic methods clearly separated the two groups of samples (i.e. ‘blood only’ from ‘blood plus bile’). However, on the pig skin the two groups could be separated by one of the photographic methods only. Separation of blood from blood and bile mixtures was obtained on the cloth and skin backgrounds using spectrophotometry and hyperspectral imaging. In a test using serial dilutions of blood and bile mixtures, the hyperspectral system performed slightly better than the spectrophotometer. The former also had the advantage of imaging a wider area and providing spatial data. Hyperspectral (chemical) imaging and spectrophotometry are superior to photography for the detection of bilirubin on a background of skin (due to the presence of yellow chromophores); this technology combined with mathematical analysis of the spectral data warrants further investigation.

APA, Harvard, Vancouver, ISO, and other styles

24

Liu, Jiang Hao, Tian Tian Mao, Qiao Liang Liu, and Shao Hong Gao. "Research on Color Matching and Reproduction on Aluminum Foil Packaging Materials." Applied Mechanics and Materials 469 (November 2013): 273–77. http://dx.doi.org/10.4028/www.scientific.net/amm.469.273.

Full text

Abstract:

The effects of aluminum foil packaging materials on color reproduction were mainly studied from the following two aspects in this topic: (1) through the study of CIEl976 chroma space, color difference evaluations of 7 kinds of colors for aluminum foil were compared with that of coated paper, based on the established color libraries of coated paper. (2) Four different spectrophotometers of X-Rite were used to measure L*、 a*、 b* of different colors, analyzing the effects of different spectrophotometer on color reproduction. Research has proved that: (1) the method which color was transferred to the aluminum foil, according to the established color libraries of coated paper, is feasible, and there was no difference between two colors on aluminum foils by visual inspection, the chromatic aberration was less than 1. (2) From the measurements, aspheric photometers like X-Rite939, Spectroeye, X-Rite500 cannot be used for color measurement on aluminum foil, the error is bigger than SP64, and accuracy is lower. (3) Through the total color difference analysis of four instruments on magenta, the ball SP64 portable spectrophotometer is the most suitable equipment for color reproduction on aluminum foil packaging materials.

APA, Harvard, Vancouver, ISO, and other styles

25

Septiani, Devi Ayu, Agus Abhi Purwoko, and Aliefman Hakim. "Solvent Characterization of Lycopene Extraction in Tomato Fruits as Sensitizer Candidates in Dye-Sensitized Solar Cell (DSSC)." Jurnal Biologi Tropis 22, no. 3 (July 19, 2022): 705–14. http://dx.doi.org/10.29303/jbt.v22i3.3787.

Full text

Abstract:

This study is an experimental study aimed at clarifying the characteristics of the solvent in the extraction of lycopene in tomato (Solanum lycopersicum) as a candidate photosensitizer for dye-sensitized solar cells (DSSC). The performance of DSSC depends on the type of dye commonly used as a sensitizer. Tomatoes contain an lycopene. Yields of lycopene extract in tomatoes were characterized by FTIR and UV-Vis spectrophotometers. The extraction methods used in this study are the maceration method and liquid-liquid extraction. The procedure of this study was carried out in two main stages, the extraction stage and the characterization stage. The first step was the extraction of lycopene from tomatoes using the maceration and liquid-liquid extraction methods. The maceration process compared the use of acetone and ethyl acetate as solvents. This extraction step produced 6.514 g (acetone) and 5.6702 g (ethyl acetate) lycopene extracts. The second step is to identify the functional groups of the compound formed using an FTIR spectrophotometer and use a UV-Vis spectrophotometer to determine the absorbance and maximum wavelength value of the lycopene and M-lycopene complex. The results of the FTIR spectrophotometer test showed that using acetone as the solvent produced wavenumbers similar to lycopene compared to ethyl acetate. UV-Vis spectrophotometer test results show the maximum wavelengths of the lycopene extract using acetone as the solvent were 447 nm, and 294 nm when ethyl acetate was used as the solvent. The Eg results revealed that the Eg values for the acetone and ethyl acetate extracts were 4.52 eV and 2.68 eV. Based on the results of property analysis of the two solvents used, acetone was more suitable than ethyl acetate for the extraction of tomato lycopene used as a DSSC sensitizer.

APA, Harvard, Vancouver, ISO, and other styles

26

Ananda, Meita Sari. "UJI KADAR SULFAT PADA AIR MINUM DALAM KEMASAN (AMDK) SECARA SPEKTROFOTOMETRI UV-VIS." AMINA 1, no. 1 (April 30, 2019): 35–38. http://dx.doi.org/10.22373/amina.v1i1.12.

Full text

Abstract:

The goal of this study is to quantitaively determine both the concentration of sulfate (SO4-2) contained in bottled-drinking water (AMDK) via spectrophotometry UV-Vis and the quality of the water compared to Permenkes No. 429/MENKES/PER/IV/2010. The sample was mixed with BaCl2 to form BaSO4 which can be recognised by employing UV-Vis spectrophotometer at 420 nm wave length. The obtained absorbance data were then applied to the linear equation proposed by using standard sulfate solution. The analysis shows that the various products of AMDK consumed Acehnese people contain sulfate in low level, numerically under 0,0397 ppm. Thie research conclude that the AMDK is safe to be consumed daily.

APA, Harvard, Vancouver, ISO, and other styles

27

NOGUEIRA, Ana Rita de Araujo, Fabiana MOCKIUTI, Gilberto Batista de SOUZA, and Odo PRIMAVESI. "Flow Injection Spectrophotometr ic Catalytic Determination of Iodine in Milk." Analytical Sciences 14, no. 3 (1998): 559–64. http://dx.doi.org/10.2116/analsci.14.559.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Budiyanto, Fitri. "GRAPHITE FURNACE ATOMIC ABSORPTION SPECTROPHOTOMETRY SEBAGAI METODE ANALISIS LOGAM BERAT." OSEANA 42, no. 3 (October 30, 2017): 9–20. http://dx.doi.org/10.14203/oseana.2017.vol.42no.3.80.

Full text

Abstract:

GRAPHITE FURNACE ATOMIC ABSORPTION SPECTROPHOTOMETRY AS A METHOD FOR ANALYZING HEAVY METAL. Heavy metal, mostly present in a (ultra) trace-level, becomes one of the hazardous pollutants due to its toxicity, bioaccumulative, biomagnification characters. Conducting a micro analysis of these trace-level pollutants, a sensitive instrument and method are strongly recommended. Graphite Furnace Atomic Absorption Spectrophotometer (GFAAS) offers a solution for the challenge. It uses the principle of light emission absorption by nascent atom cloud from the metals. The analysis has been developed in three stages of the tube heating programs, i.e. drying, ashing and then atomizing element at 3000° C. Since the injected sample is almost completely atomized, the sensitivity of GFAAS is extremely high and this value is covering up the disadvantages of the other analyses. Therefore, the GFAAS analysis is suitable fo ultra-trace analysis of ultra-trace pollutants like heavy metals in environment.

APA, Harvard, Vancouver, ISO, and other styles

29

Sari, Indah, Robiyatul Adawiyah, and Nurhidayanti Nurhidayanti. "PERBEDAAN HASIL KADAR FORMALIN PADA SAMPEL TAHU YANG DIRENDAM AIR HANGAT DAN AIR GARAM MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS." JURNAL MEDIA KESEHATAN 15, no. 2 (December 27, 2022): 140–53. http://dx.doi.org/10.33088/jmk.v15i2.857.

Full text

Abstract:

Problems: Formalin Examination is use to determine of presence or absence of harmful substances or preservatives in samples tested or examined on food, especially tofu. The Aim Of The Research: To determine the difference in the results of formaldehyde levels in tofu samples soaked in warm water and salt water using UV-Vis Spectrophotometry method. Research Method: The type of research used was a pure experiment, which was conducted at the Laboratory of Chemistry and Microbiology of Agricultural Products, Department of Agricultural Technology, Faculty of Agriculture, Unsri Palembang. The sample used was tofu and the number of careful samples was 18 tofu samples using the total sampling technique. The Result: Based on the results of examining the formalin content in tofu samples soaked in warm water for 15 minutes using the UV-Vis Spectrophotometry method with the A & E Visible Spectrophotometer yielded an average of 0.56 mg/L while the results of examining formalin levels in tofu samples soaked in salt water produces an average of 0.74 mg/L. Data analysis using the Independent Sample T-Test test obtained a significant value for the warm water treatment p = 0.395 and a significant value for the salt water treatment p = 0.397. Conclutions: There are differences in the results of formalin levels in tofu samples soaked in warm water and salt water using UV-Vis Spectrophotometry method.

APA, Harvard, Vancouver, ISO, and other styles

30

Siomos, Nikolaos, Dimitrios Balis, Alkiviadis Bais, Mariliza Koukouli, Katerina Garane, Kalliopi A. Voudouri, Fani Gkertsi, Athanasios Natsis, Dimitrios Karagkiozidis, and Ilias Fountoulakis. "Towards an Algorithm for Near Real Time Profiling of Aerosol Species, Trace Gases, and Clouds Based on the Synergy of Remote Sensing Instruments." EPJ Web of Conferences 237 (2020): 08023. http://dx.doi.org/10.1051/epjconf/202023708023.

Full text

Abstract:

In this manuscript we present the concept of a novel algorithmic chain that aims to a dataset of unprecedented detail in the vertical distribution of multiple atmospheric components in near real time conditions. The analysis will be based on the following remote sensing instruments: a depolarization Raman lidar, a visible and a thermal all-sky camera, a Brewer spectrophotometer, and up to three mini DOAS/MAX-DOAS systems. Based on both individual and synergistic processing of the data collected, novel products will be made available in near real time conditions to the end users. Columnar aerosol information from the spectrophotometers will be combined with lidar data to retrieve vertical profiles of individual aerosol species. Cloud layers will be detected and classified based mainly on the synergy of the lidar and the sky cameras and a realistic 3D representation of cloud conditions around the measurement site will be produced. Lidar profiles will be implemented as a priori information for radiative transfer purposes, that are necessary in order to obtain high quality trace gases profiles from the DOAS/MAX-DOAS spectrophotometer. Fast synergistic data processing will ensure that the algorithm can be applied for near real time public data dissemination in the future.

APA, Harvard, Vancouver, ISO, and other styles

31

HEMASUNDARAM, Abburi, and Nutalapati Venkatasubba NAIDU. "Sensitive Determination of Propoxur by Spectrophotometry Using 3-Aminopyridine." Analytical Sciences 20, no. 12 (2004): 1707–10. http://dx.doi.org/10.2116/analsci.20.1707.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

EL-SAYED, Abdel-Aziz Y., and Najeb A. EL-SALEM. "Recent Developments of Derivative Spectrophotometry and Their Analytical Applications." Analytical Sciences 21, no. 6 (2005): 595–614. http://dx.doi.org/10.2116/analsci.21.595.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

SHAH, Jasmin, M. Rasul JAN, and Nadia BASHIR. "Determination of Starane (Fluroxypyr) Herbicide Using Flow Injection Spectrophotometry." Analytical Sciences 22, no. 1 (2006): 145–46. http://dx.doi.org/10.2116/analsci.22.145.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

ZHANG, Jinzhong, Wanzhi WEI, Youan MAO, Wei LAN, and Mingjun CHEN. "A New Method for a Mutagenicity Test Using Spectrophotometry." Analytical Sciences 16, no. 12 (2000): 1265–69. http://dx.doi.org/10.2116/analsci.16.1265.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Widodo, Didik Setiyo, Rahmad Nuryanto, Abdul Haris, Prihastuti Santini Laksmi Dewi, and Lutfia Apipah. "Potential Oxidative Treatment Using Pb-PbO2 Electrode in Electrodecolorizing Batik Wastewater." Jurnal Kimia Sains dan Aplikasi 21, no. 3 (July 22, 2018): 118–23. http://dx.doi.org/10.14710/jksa.21.3.118-123.

Full text

Abstract:

Electrodecolorization of batik wastewater has been done. Study was performed to remediate aquatic environment containing dyes of batik industry by electrolysis, decreasing COD and some metal ions as well. Research was conducted by electrolyzing sample from Buaran Pekalongan and Semarang using Pb and PbO2 as electrodes at a constant applied potential. Solution after treating were analysed, zeolite sieving treatment, and final analysis by UV-Vis spectrophotometer for measuring decolorization percentages, atomic absorption spectrophotometerer (AAS) for evaluating metal ions of Cu2+, total Fe and Na+, and COD analysis. Results show that electrolysis of batik wastewater using PbO2 as anode effectively decolorize and decreasing COD values. Sample containing indigozol and remazol black B (RBB) were decolorized up to 100%. CODs were reduced to 98.6% and 95.4%, respectively. Zeolite treatment enhancing water quality by reducing ion concentration of Cu2+, total Fe ions and Na+.

APA, Harvard, Vancouver, ISO, and other styles

36

Garg, Anuj Kumar. "Quantitative Analysis of Caffeine in the Green Tea, Black Tea and Soft Drink Using UV-Visible Spectrophotometer." Indian Journal of Science and Technology 14, no. 37 (October 5, 2021): 2860–64. http://dx.doi.org/10.17485/ijst/v14i37.2241.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

EL-SAYED, Abdel-Aziz Y., Yehia Z. HUSSEIN, and Montaser A. MOHAMMED. "Simultaneous Determination of Phosphate and Silicate by First-Derivative Spectrophotometry." Analytical Sciences 17, no. 12 (2001): 1461–64. http://dx.doi.org/10.2116/analsci.17.1461.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

ABBASPOUR, Abdolkarim, and Leila BARAMAKEH. "Simultaneous Determination of Zirconium and Molybdenum by First-Derivative Spectrophotometry." Analytical Sciences 18, no. 10 (2002): 1127–30. http://dx.doi.org/10.2116/analsci.18.1127.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Blanco, M., J. Coello, H. Iturriaga, S. Maspoch, and M. Redon. "Principal Component Regression for Mixture Resolution in Control Analysis by UV-Visible Spectrophotometry." Applied Spectroscopy 48, no. 1 (January 1994): 37–43. http://dx.doi.org/10.1366/0003702944027633.

Full text

Abstract:

The potential of principal component regression (PCR) for mixture resolution by UV-visible spectrophotometry was assessed. For this purpose, a set of binary mixtures with Gaussian bands was simulated, and the influence of spectral overlap on the precision of quantification was studied. Likewise, the results obtained in the resolution of a mixture of components with extensively overlapped spectra were investigated in terms of spectral noise and the criterion used to select the optimal number of principal components. The model was validated by cross-validation, and the number of significant principal components was determined on the basis of four different criteria. Three types of noise were considered: intrinsic instrumental noise, which was modeled from experimental data provided by an HP 8452A diode array spectrophotometer; constant baseline shifts; and baseline drift. Introducing artificial baseline alterations in some samples of the calibration matrix was found to increase the reliability of the proposed method in routine analysis. The method was applied to the analysis of mixtures of Ti, AI, and Fe by resolving the spectra of their 8-hydroxyquinoline complexes previously extracted into chloroform.

APA, Harvard, Vancouver, ISO, and other styles

40

Popova, Marina I., Tatyana A. Kobeleva, and Alik I. Sichko. "Spectrophotometric analysis of carvedilol in a medication based on aquacomplex of titanium glycerosolvate." Aspirantskiy Vestnik Povolzhiya 22, no. 2 (November 1, 2022): 67–72. http://dx.doi.org/10.55531/2072-2354.2022.22.2.67-72.

Full text

Abstract:

Aim to develop a methodology for quantitative spectrophotometric analysis of carvedilol in the "Carvedilosol" medication based on aquacomplex of titanium glycerosolvate. Material and methods. During the analysis we used the pharmaceutical substance "Carvedilol", gel "Tizol", ointment "Carvedilosol" consisting of 0.5% beta-blocker in titanium glycerohydrogel. The spectrophotometry was applied, registration of optical density and electron spectra was performed on spectrophotometer SF-2000. The following parameters were chosen for validation of the methodology: specificity, linearity, precision, correctness. Results. It is reasonable to analyze Carvedilol at a wavelength of 243 nm with the limit of quantitative determination of 0.761 g/ml. The results of the study showed that the weight of carvedilol in the ointment is within acceptable limits (0.0404-0.0548 g) according to the regulatory documentation for soft dosage forms (Order of the Ministry of Health of the Russian Federation No. 751n dated 26.10.2015). Conclusion. Optimal conditions for the implementation of spectrophotometric method of analysis were selected for detection of the content of carvedilol in the ointment "Carvedilosol" with a relative error not exceeding 1.80 %.

APA, Harvard, Vancouver, ISO, and other styles

41

MATSUO, Koichi, Kenichi SAKAI, Yosuke MATSUSHIMA, Takayuki FUKUYAMA, and Kunihiko GEKKO. "Optical Cell with a Temperature-Control Unit for a Vacuum-Ultraviolet Circular Dichroism Spectrophotometer." Analytical Sciences 19, no. 1 (2003): 129–32. http://dx.doi.org/10.2116/analsci.19.129.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Peng, Fei, Shi Bo Liu, Hai Yan Xu, and Zhi Qun Li. "A Comparative Study on the Analysis Methods for Chlorophyll-a." Advanced Materials Research 726-731 (August 2013): 1411–15. http://dx.doi.org/10.4028/www.scientific.net/amr.726-731.1411.

Full text

Abstract:

This paper investigated three chlorophyll-a analysis methods in water sample. The UV-VIS spectrophotometry method followed SL88-2012 standard procedure, the results showed the detection limit of chlorophyll-a was 0.22 μg/L, with the lower detection limit of 1.0 μg/L. The detection range of the vivo chlorophyll-a fluorescence method was 0.1-300μg/L, the ratios of different algae matched that prepared in water samples. The detection limits and quantification limits for HPLC by using UVD and FLD were respectively 0.013 and 0.004 μg/L, 0.040 and 0.013μg/L. FLD was more sensitive than UVD for chlorophyll-a analysis, and the recovery of standard addition was in the range of 90.2% to 103.9% with UVD and FLD. Given the different pretreatment procedures of the sample, equipment conditions, correction and precision, vivo chlorophyll-a fluorescence method was the best on-site monitoring and the emergency monitoring method. Spectrophotometer was suitable for routine laboratory determination of chlorophyll-a, especially for bulk water samples. HPLC method had high precision and sensitivity, so this method applied to the determination of exact requirements, microanalysis and pigment separation.

APA, Harvard, Vancouver, ISO, and other styles

43

Kosenkova, S. I., I. I. Krasnyuk, I. I. Krasnyuk (jr.), A. V. Belyatskaya, O. I. Stepanova, and S. R. Naryshkin. "Evaluation of the Possibility of Using the Method of UV Spectrophotometry for the Development of Quantitative Determination of Naftifine Hydrochloride in its Solution with a Combination of PEG for the Treatment of Fungal Infections." Drug development & registration 9, no. 1 (February 26, 2020): 35–38. http://dx.doi.org/10.33380/2305-2066-2020-9-1-35-38.

Full text

Abstract:

Introduction. An innovative antifungal agent based on hydrochloride with combination of PEG-400 and PEG-1000 has been developed based at the chairs of Sechenov University. This solution is designated for external application as an antifungal agent. Naftifine hydrochloride has a broad spectrum of action against various fungi capable of causing onychomycosis. PEGs, forming part of the developed dosage form, bring about necessary viscosity of the solution (for retention in application area) and do not prevent drug product performance. Naftifine hydrochloride has an apparent UV absorption band with maximum of 256 ± 2 mn, in connection with this, it is supposed to use the UV spectrophotometry method for further development of quantitation method of naftifine hydrochloride detection in tested oral liquid.Aim. To evaluate the feasibility of using the UV spectrophotometry method for development of quantitation of naftifine hydrochloride in its solution with a combination of PEG for mycotic infection treatment.Materials and methods. The substance of naftifine hydrochloride, PEG-400 and PEG-1000, ethyl alcohol 96 %, UV spectrophotometry, «Millipore» filter.Results and discussion. A set of operations was carried out with UV spectrophotometer, including trials of solutions of initial substance of naftifine hydrochloride, PEG solutions, a solution of developed prolonged antimycotic drug and its placebo. The identity of UV spectra of the actual substance in various media was determined, maximum absorption of actual substance and dependence preservation between specified concentration and optical density of the solution were proved. It was found that auxiliary substances included in liquid dosage form do not affect the main characteristics of the UV spectrum of actual substance.Conclusion. The possibility in principle of using the UV spectrophotometry method for quantitative and qualitative analysis of innovative prolonged antimycotic drug – liquid dosage form of naftifine hydrochloride with a combination of PEG has been established.

APA, Harvard, Vancouver, ISO, and other styles

44

Syamsu Nur, Andi Nur Aisyah, Alfat Fadri, Sharfianty, Amriani Sapra, and Fitriyanti Jumaetri Sami. "Comparative study of catechin levels from green tea, oolong tea and black tea product with various treatments." GSC Biological and Pharmaceutical Sciences 14, no. 1 (January 30, 2021): 001–10. http://dx.doi.org/10.30574/gscbps.2021.14.1.0416.

Full text

Abstract:

Background: Tea is a refreshing drink that contains polyphenol compounds, namely catechins that are used for medicine and cosmetics. This study was to assess the content of catechin compounds in green tea, oolong and black tea products from Indonesia, China and Taiwan. Methods: Some tea products are brewed at varying temperatures (75±2; 85±2 and 95±20 C) and times (5; 10 and 15 minutes). Identification of catechin compounds was carried out using chemical reagents and UV spectrophotometry. The level of cathecin in tea products were analyzed by spectrophotometer at 280 nm wavelength. Results: The results obtained indicate that green tea, oolong tea and black tea contain epigallocatechin-3-gallate (EGCG) compounds according to the color change based on chemical reagents and for UV spectrum analysis which has λmax in the range 268-274 nm. The results of quantitative tests using UV-Vis spectrophotometry showed that the green tea samples gave the highest levels of catechins followed by oolong tea and black tea with brewing temperature at 95±20 °C. Conclusion: The catechin content of tea obtained from various products varies according to the type of processing method and the brewing temperature. Therefore, this study is expected to provide information related to catechin content to the public and researchers.

APA, Harvard, Vancouver, ISO, and other styles

45

LONNI, Audrey A. S. G., Ieda S. SCARMINIO, Lucas M. C. SILVA, and Dalva T. FERREIRA. "Numerical Taxonomy Characterization of Baccharis Genus Species by Ultraviolet-Visible Spectrophotometry." Analytical Sciences 21, no. 3 (2005): 235–39. http://dx.doi.org/10.2116/analsci.21.235.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Barges, N., H. Conan, and N. Poisson. "NIR spectrophotometry: Qualitative and quantitative applications in pharmaceutical industry (in French)." Analusis 26, no. 4 (May 1998): 43–45. http://dx.doi.org/10.1051/analusis:199826040043.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Rohmah, Siti Awwalul Amanatur, Afidatul Muadifah, and Rahma Diyan Martha. "Validasi Metode Penetapan Kadar Pengawet Natrium Benzoat pada Sari Kedelai di Beberapa Kecamatan di Kabupaten Tulungagung Menggunakan Spektrofotometer Uv-Vis." Jurnal Sains dan Kesehatan 3, no. 2 (April 30, 2021): 120–27. http://dx.doi.org/10.25026/jsk.v3i2.265.

Full text

Abstract:

Sodium benzoate is an organic preservative chemical compound which use is permitted if the amount is below the maximum threshold. This research aims to validate the UV-Vis Spectrophotometry method which will be used to determine sodium benzoate levels in soybean milk in 3 sub-districts in the Tulungagung Regency using the UV-Vis Spectrophotometer instruments. Before determining the content, the samples were analyzed qualitatively first using the acid-base titration method. The result of this qualitative analysis of the sample will turn pink if it contains sodium benzoate. Then the sodium benzoate wavelength optimization is carried out in the range of 200-400nm, and the optimum wavelength is 226nm. The method validation process is done by using four parameters namely linearity test, accuracy test, precision test, and LOD&LOQ. Based on the validation of the method, the correlation coefficient (R2) of 0.99563 indicates linear, recovery % is 97.58% in the range of 80-120%, RSD is 0.0454% which is ?2%, LOD is 0.33 ppm, and LOQ of 1.0996 ppm. Based on these results, it can be said that the method used is valid because all parameters meet the specified requirements. Analysis of the determination of levels using a UV-Vis Spectrophotometer instruments at wavelength 226nm using 5 samples, from the five samples obtained average rate of 90.639±0.0406. Analysis of the rate determination using UV-Vis Spectrophotometer instruments at 226nm wavelengths show that sample A has a concentration 92,243±0,039 ppm, sample B is 80,286±0,039 ppm, sample C is 99,04±0,063 ppm, sample D is 101,483±0,025 ppm, and sample E is 80,143±0,038 ppm. The content of sodium benzoate in soybean milk is following the requirements of BPOM RI regulation No.36 of 2013 concerning the maximum limit of food use, the use of sodium benzoate in fruit/vegetable juice products and non-fermented soybean products is 600ppm of food, with ADI 0-5mg/kg body weight.

APA, Harvard, Vancouver, ISO, and other styles

48

Agustina, Agustina, Munawarah Munawarah, Stefanus Agustinus Lumi, and Syamsu Nur. "Green Synthesis Nanopartikel Perak (Agnps) Terkonjugasi Etil Parametoksi Sinamat (Epms) sebagai Bahan Tabir Surya." Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) 4, no. 2 (October 20, 2018): 98–105. http://dx.doi.org/10.22487/j24428744.2018.v4.i2.10440.

Full text

Abstract:

Green Synthesis is a method to make silver nanoparticles (AgNPs) by utilizing natural materials as bioreductors. One of the natural materials that can reduce metal ions is Kaempferia galanga rhizome because it has a chemical component that can penetrate the metal. The compounds are ethyl paramethoxycinnamate (EPMC) which is used as sunscreen. This study aims to produce AgNPs conjugated with EPMC that can be used as raw material sunscreen. This study was conducted by extracting EPMS from the Kaempferia galanga rhizome and synthesizing the AgNPs conjugated with EPMC. The synthesis results were characterized by UV-Vis Spectrophotometry, FTIR, SEM, PSA and sunscreen activity. Extracted result was obtained crystalline is ethyl paramethoxycinnamate compound with yield 2,3%. The characterization of EPMC with UV-Vis spectrophotometer was obtained maximum wavelength of 308 nm and FTIR analysis result at wave number 1701,27 cm-1 indicated the presence of carbonyl group (C = O) and 1165,97 cm-1 presence of C-O and showed specific characteristic from EPMS. Characterization of AgNPs using Uv-Vis spectrophotometer was obtained maximum wavelength of 469 nm. Characterization of AgNPs using SEM are spherical and monodispers forms. Then, particle size using PSA obtained an average diameter of 182 nm. AgNPs conjugated with EPMS has a sunscreen activity with sunblock category and successfully provide protection against UV exposure at low concentration (200 ppm) categorized as ultra protection with SPF value 36,4.

APA, Harvard, Vancouver, ISO, and other styles

49

Ngibad, Khoirul, and Dheasy Herawati. "Perbandingan Pengukuran Kadar Vitamin C Menggunakan Spektrofotometri UV-Vis pada Panjang Gelombang UV dan Visible." Borneo Journal of Medical Laboratory Technology 1, no. 2 (April 30, 2019): 77–81. http://dx.doi.org/10.33084/bjmlt.v1i2.715.

Full text

Abstract:

Vitamin C is a source of antioxidants which has a primary function to inhibit the occurrence of an oxidation reaction and inhibit a free radical. The source of vitamin C can be found in food and beverage. This study aims to compare the measurement of vitamin C levels using a UV-Vis spectrophotometer at UV and Visible wavelength. The method used in this study was the UV-Vis spectrophotometry method. The analysis of vitamin C levels at UV wavelength 266 nm and Visible wavelength 494 nm with a calibration curve range 0.3 to 0.8 mg/L. Validation of the methods studied included: linearity, detection limits, quantization limits, and precision. The results showed that the measurement of vitamin C levels at UV wavelengths resulted in the linearity of concentrations of 0.2 - 0.8 mg / L, the limit of detection of 0.05 mg/L, the limit of quantization of 0.17 mg/L and level of precision with RSD to 0.2015. On the other hand, the measurement of vitamin C levels in the Visible wavelength produced linearity from concentrations of 0.3 to 0.8 mg/L, the detection limit of 0.05 mg/L, quantization limit of 0.18 mg/L and the level of precision with RSD of 1.0489. Thus, the determination of vitamin C levels in samples using a UV-Vis spectrophotometer was preferred to be measured at UV wavelengths.

APA, Harvard, Vancouver, ISO, and other styles

50

Meyns, Silke, Richard Illi, and Bruno Ribi. "Comparison of chlorophyll-a analysis by HPLC and spectrophotometry: Where do the differences come from?" Archiv für Hydrobiologie 132, no. 2 (December 21, 1994): 129–39. http://dx.doi.org/10.1127/archiv-hydrobiol/132/1994/129.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Spectrophotometer analysi'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles