Academic literature on the topic 'Spatial hybridization'
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Journal articles on the topic "Spatial hybridization"
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Klein, Etienne K., Lélia Lagache-Navarro, and Rémy J. Petit. "Demographic and spatial determinants of hybridization rate." Journal of Ecology 105, no. 1 (December 16, 2016): 29–38. http://dx.doi.org/10.1111/1365-2745.12674.
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Omilani, Nathaniel A., and Shamsudeen Adebayo Raji. "Effect of Computer Animation Instructional Package on Students’ Achievement in Hybridization in Chemistry." International Journal of Instruction 17, no. 3 (July 1, 2024): 435–52. http://dx.doi.org/10.29333/iji.2024.17324a.
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The study aimed to ascertain how learners' performance in the chemical hybridization concept was affected by the Computer Animation Instructional Package (CAI). A pretest-posttest quasi-experimental research design was used in the study. There were 120 seniors secondary school students in the sample. The control group received instruction via traditional lectures, while the experimental group was treated using computer animation. The Hybridization Achievement Test (HAT) and Students' Spatial Ability Test (SSAT) were tested and used for data collection. The tests had reliability coefficients of 0.71 and 0.77, respectively using Kuder Richardson 20. Using estimated marginal means and Analysis of Covariance (ANCOVA) at a 0.05 significance level, seven research hypotheses were tested. Results showed that CAI highly impacted students' performance in the chemical concept of hybridization. A noteworthy interaction effect between gender and spatial ability was also found, favouring females with high spatial ability over males with low spatial ability regarding learners' performance. The research results indicate that CAI enhanced students' performance on the hybridization concept in chemistry. The results also showed the gender-friendly and successful instructional characteristics of CAI.
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Lowe, Winsor H., Clint C. Muhlfeld, and Fred W. Allendorf. "Spatial sorting promotes the spread of maladaptive hybridization." Trends in Ecology & Evolution 30, no. 8 (August 2015): 456–62. http://dx.doi.org/10.1016/j.tree.2015.05.008.
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Voith von Voithenberg, Lena, Anna Fomitcheva Khartchenko, Deborah Huber, Peter Schraml, and Govind V. Kaigala. "Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity." Nucleic Acids Research 48, no. 3 (December 19, 2019): e17-e17. http://dx.doi.org/10.1093/nar/gkz1151.
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Abstract Multiplexed RNA in situ hybridization for the analysis of gene expression patterns plays an important role in investigating development and disease. Here, we present a method for multiplexed RNA-ISH to detect spatial tumor heterogeneity in tissue sections. We made use of a microfluidic chip to deliver ISH-probes locally to regions of a few hundred micrometers over time periods of tens of minutes. This spatial multiplexing method can be combined with ISH-approaches based on signal amplification, with bright field detection and with the commonly used format of formalin-fixed paraffin-embedded tissue sections. By using this method, we analyzed the expression of HER2 with internal positive and negative controls (ActB, dapB) as well as predictive biomarker panels (ER, PgR, HER2) in a spatially multiplexed manner on single mammary carcinoma sections. We further demonstrated the applicability of the technique for subtype differentiation in breast cancer. Local analysis of HER2 revealed medium to high spatial heterogeneity of gene expression (Cohen effect size r = 0.4) in equivocally tested tumor tissues. Thereby, we exemplify the importance of using such a complementary approach for the analysis of spatial heterogeneity, in particular for equivocally tested tumor samples. As the method is compatible with a range of ISH approaches and tissue samples, it has the potential to find broad applicability in the context of molecular analysis of human diseases.
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Bassell, Gary, and Robert H. Singer. "Ultrastructural analysis of the spatial distribution of MRNA." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 1 (August 1992): 552–53. http://dx.doi.org/10.1017/s0424820100123167.
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We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.
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Hitt, Nathaniel P., Christopher A. Frissell, Clint C. Muhlfeld, and Fred W. Allendorf. "Spread of hybridization between native westslope cutthroat trout, Oncorhynchus clarki lewisi, and nonnative rainbow trout, Oncorhynchus mykiss." Canadian Journal of Fisheries and Aquatic Sciences 60, no. 12 (December 1, 2003): 1440–51. http://dx.doi.org/10.1139/f03-125.
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We examined spatial and temporal patterns of hybridization between native westslope cutthroat trout, Oncorhynchus clarki lewisi, and nonnative rainbow trout, O. mykiss, in streams of the Flathead River system in Montana, U.S.A. We detected hybridization in 24 of 42 sites sampled from 1998 to 2001. We found new Oncorhynchus mykiss introgression in seven of 11 sample populations that were determined to be nonhybridized in 1984. Patterns of spatial autocorrelation and linkage disequilibrium indicated that hybridization is spreading among sites and is advancing primarily via post-F1 hybrids. Although hybridized populations were distributed widely throughout the study area, the genetic contribution from O. mykiss decreased with increasing upstream distance from the Flathead River mainstem, suggesting that O. mykiss introgression is spreading in an upstream direction. The spread of hybridization may be constrained more by demographic than by environmental factors, given that (i) hybridized populations generally encompassed the range of environmental variability in nonhybridized populations, and (ii) hybridization status was more strongly associated with neighborhood statistics than measured environmental gradients.
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Serhal, Philippe, and Sébastien Lemieux. "Correction of Spatial Bias in Oligonucleotide Array Data." Advances in Bioinformatics 2013 (March 13, 2013): 1–9. http://dx.doi.org/10.1155/2013/167915.
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Background. Oligonucleotide microarrays allow for high-throughput gene expression profiling assays. The technology relies on the fundamental assumption that observed hybridization signal intensities (HSIs) for each intended target, on average, correlate with their target’s true concentration in the sample. However, systematic, nonbiological variation from several sources undermines this hypothesis. Background hybridization signal has been previously identified as one such important source, one manifestation of which appears in the form of spatial autocorrelation. Results. We propose an algorithm, pyn, for the elimination of spatial autocorrelation in HSIs, exploiting the duality of desirable mutual information shared by probes in a common probe set and undesirable mutual information shared by spatially proximate probes. We show that this correction procedure reduces spatial autocorrelation in HSIs; increases HSI reproducibility across replicate arrays; increases differentially expressed gene detection power; and performs better than previously published methods. Conclusions. The proposed algorithm increases both precision and accuracy, while requiring virtually no changes to users’ current analysis pipelines: the correction consists merely of a transformation of raw HSIs (e.g., CEL files for Affymetrix arrays). A free, open-source implementation is provided as an R package, compatible with standard Bioconductor tools. The approach may also be tailored to other platform types and other sources of bias.
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Gyllborg, Daniel, Christoffer Mattsson Langseth, Xiaoyan Qian, Eunkyoung Choi, Sergio Marco Salas, Markus M. Hilscher, Ed S. Lein, and Mats Nilsson. "Hybridization-based in situ sequencing (HybISS) for spatially resolved transcriptomics in human and mouse brain tissue." Nucleic Acids Research 48, no. 19 (September 29, 2020): e112-e112. http://dx.doi.org/10.1093/nar/gkaa792.
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Abstract Visualization of the transcriptome in situ has proven to be a valuable tool in exploring single-cell RNA-sequencing data, providing an additional spatial dimension to investigate multiplexed gene expression, cell types, disease architecture or even data driven discoveries. In situ sequencing (ISS) method based on padlock probes and rolling circle amplification has been used to spatially resolve gene transcripts in tissue sections of various origins. Here, we describe the next iteration of ISS, HybISS, hybridization-based in situ sequencing. Modifications in probe design allows for a new barcoding system via sequence-by-hybridization chemistry for improved spatial detection of RNA transcripts. Due to the amplification of probes, amplicons can be visualized with standard epifluorescence microscopes for high-throughput efficiency and the new sequencing chemistry removes limitations bound by sequence-by-ligation chemistry of ISS. HybISS design allows for increased flexibility and multiplexing, increased signal-to-noise, all without compromising throughput efficiency of imaging large fields of view. Moreover, the current protocol is demonstrated to work on human brain tissue samples, a source that has proven to be difficult to work with image-based spatial analysis techniques. Overall, HybISS technology works as a targeted amplification detection method for improved spatial transcriptomic visualization, and importantly, with an ease of implementation.
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Tompsett, A. R., J. W. Park, X. Zhang, P. D. Jones, J. L. Newsted, D. W. T. Au, E. X. H. Chen, et al. "In situ hybridization to detect spatial gene expression in medaka." Ecotoxicology and Environmental Safety 72, no. 4 (May 2009): 1257–64. http://dx.doi.org/10.1016/j.ecoenv.2008.10.013.
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Neole, Bhumika, Latika Pinjarkar, Jagdish Chandra Patni, Anusha Vidyabhanu, Anshu Malpani, and Ojas Dudhe. "Denoising of Digital Images Using Spatial Domain Edge Detection Approach." International Journal of Religion 5, no. 6 (April 29, 2024): 298–307. http://dx.doi.org/10.61707/y562qn51.
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Better results can be produced by the Hybridization of the Wavelet-based image denoising technique and sparse representation of edges. A novel method for spatial domain edge identification that produces a denoised image that has been tainted by additive white Gaussian noise without sacrificing the image's detail information. By combining bivariate shrinkage and local profile edge detection, a denoised image is produced. In this paper, the hybridization method is proposed by modifying the existing Wavelet Transform for image denoising leading to an increase in the PSNR and SSIM as compared to that given by existing Wavelet denoising techniques, maintaining the visual quality of an image. To modify the wavelet coefficients Bivariate Wavelet Shrinkage is used. The quality assessment is evaluated in terms of SSIM value and PSNR value.
Dissertations / Theses on the topic "Spatial hybridization"
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Tang, Siu-hang Wesley. "Beyond hybridization the spatial histories of Mong Kok, Hong Kong /." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B30712440.
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Tang, Siu-hang Wesley, and 鄧肇恆. "Beyond hybridization: the spatial histories of Mong Kok, Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B30712440.
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Yau, Monica M. "Hybridization in western trout : spatial variation and the role of environmental factors." Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/45527.
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Hybridization and introgression with non-native salmonids is one of the greatest factors threatening native cutthroat trout species. Westslope cutthroat trout (Oncorhynchus clarkii lewisi; WSCT) were recently listed under the Canadian Species at Risk Act (SARA) as "special concern" (British Columbia populations) and "threatened" (Alberta populations). I employed a 10 locus-microsatellite DNA assay to investigate levels of hybridization between westslope cutthroat trout and introduced rainbow trout (O. mykiss; RT) at 159 sampling locations in southwestern Alberta and parts of southeastern British Columbia. My results revealed that hybridization is extensive across the region sampled. Admixture levels (qwsct of 0 = pure rainbow trout, 1.0 = pure westslope cutthroat trout) at sampling locations ranged from 0.01 to 0.99. An average qwsct below 0.99 is a criterion that has been used in previous work to designate a population as "hybridized." Landscape genetic analysis using regression trees indicate that water temperature, elevation, distance to the nearest stocking site and distance to the nearest railway were significant components of a model that described 34% of the variation in qwsct across 58 sites for which habitat variables were available. Building on this finding, I explored the role of water temperature, the best predictor of hybridization levels amongst the variables tested, in limiting the spread of admixture by evaluating cold tolerance in both species using critical thermal methods (CTM). Analysis of variance revealed a statistically significant difference between the critical thermal minima (CTMin) of WSCT and RT acclimated to 15 °C (1.0 ± 0.8 °C and 1.4 ± 1.0 °C respectively). The heritability of cold tolerance observed in this study appears to be complex and does not seem to behave in a simple additive manner. The identification of water temperature as a major factor influencing admixture and subsequent test for physiological differences in cold tolerance provide evidence to support a hypothesis that cold water habitats act as a natural barrier to hybridization between WSCT and RT. This information provides insight into the evolutionary history of WSCT and RT and will be useful in assisting conservation efforts aimed at mitigating the wide-spread loss of WSCT to genomic extinction.
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Bassell, Gary J. "Development and Application of Ultrastructural in Situ Hybridization to Visualize the Spatial Organization of mRNA: a Dissertation." eScholarship@UMMS, 1992. https://escholarship.umassmed.edu/gsbs_diss/153.
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It has been well documented that mRNA is associated with the cytoskeleton, and that this relationship is involved in translation and mRNA sorting. The molecular components involved in the attachment of mRNA to the cytoskeleton are only poorly understood. The objective of this thesis was to directly visualize the interaction of mRNA with the cytoskeleton, with sufficient resolution to identify the filament systems and structures involved. This work required the development of novel in situ hybridization methods for use with electron microscopy. This allowed resolution to visualize single mRNA molecules and individual filaments.
The development of a silver enhancement methodology for both the light and electron microscopic detection of biotinated oligo-dT probes permitted a synoptic view of the intracellular distribution of poly(A) mRNA. At the light microscope, the distribution of poly(A) mRNA did not resemble the individual distribution patterns of microfilaments, intermediate filaments or microtubules. Ultrastructural examination revealed that poly(A) mRNA was not uniformly distributed along cytoskeletal filaments, but clustered at their intersections. The composition of these mRNA containing structures was investigated by both morphologic and in situ hybridization analysis using antibodies to cytoskeletal proteins. In thin sections, polysomes were observed attached to both microfilaments and intermediate filaments. To permit the simultaneous detection of oligo-dT hybridization and specific cytoskeletal proteins, a double labelling method using colloidal gold conjugated antibodies was developed. The majority of poly(A) mRNA was associated with the actin cytoskeleton, with 72% of the hybridization localized within 5nm of a labelled microfilament. Within the actin cytoskeleton, poly(A) mRNA was localized to intersections of orthogonal networks. Greater than 50% of poly(A) colocalized with the actin crosslinking proteins, filamin and α-actinin, but not vinculin.
A significant amount of poly(A) mRNA was found to be associated with intermediate filaments. The double label gold analysis demonstrated that 33% of the hybridization signal localized within 5nm of labelled vimentin filaments. Prior disorganization of the actin cytoskeleton using cytochalasin did not disrupt the association of mRNA with vimentin. These observations are consistent with our morphologic results of polysome-intermediate filament associations, and indicate that microfilaments are not the only filament system to which mRNA is bound. Furthermore, a small amount of hybridization signal (12%) consistently was observed along microtubules, providing an additional cytoskeletal network to distribute mRNA.
To further characterize the spatial organization of mRNA within the cytoskeleton, ultrastructural methods were developed to directly visualize individual mRNA molecules. First, oligonucleotide probes chemically modified with a single hapten and directly conjugated primary reagents were used to permit detection of an individual hybridized probe molecule by a single gold particle. Second, biotin and digoxigenin labelled oligonucleotide probes were used to simultaneously visualize the intermolecular and intramolecular relationships of two nucleic acid sequences. Third, reverse transcriptase was used to extend hybridized primers in situ which permitted visualization of the poly(A) sequence concomittant with the conformation of an mRNA molecule. These methods have permitted analysis of how single mRNA molecules may be positioned with respect to each other within the cytoskeleton.
The ultrastructural visualization of mRNA within its structural environment has demonstrated heterogeneous interactions with the cytoskeleton. Future work will be needed to further characterize the mechanism of mRNA attachment. The proteins which bridge nucleic acid sequences to specific intersections can be identified. It will be interesting to learn how the identified mRNA-cytoskeletal interactions might be involved in the regulation of both mRNA translation and intracellular location. Lastly, and perhaps the most challenging goal, is to investigate whether the identified mRNA-cytoskeletal interactions are used by the cell to influence its own shape, polarity and architecture.
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Dubrowski, Piotr. "An automated multicolour fluorescence in situ hybridization workstation for the identification of clonally related cells." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/733.
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The methods presented in this study are aimed at the identification of subpopulations (clones) of genetically similar cells within tissue samples through measurement of loci-specific Fluorescence in-situ hybridization (FISH) spot signals for
each nucleus and analyzing cell spatial distributions by way of Voronoi tessellation and Delaunay triangulation to robustly define cell neighbourhoods.
The motivation for the system is to examine lung cancer patient for
subpopulations of Non-Small Cell Lung Cancer (NSCLC) cells with biologically meaningful gene copy-number profiles: patterns of genetic alterations statistically
associated with resistance to cis-platinum/vinorelbine doublet chemotherapy treatment.
Current technologies for gene-copy number profiling rely on large amount of cellular
material, which is not always available and suffers from limited sensitivity to only the
most dominant clone in often heterogeneous samples. Thus, through the use of FISH, the
detection of gene copy-numbers is possible in unprocessed tissues, allowing identification of specific tumour clones with biologically relevant patterns of genetic aberrations.
The tissue-wide characterization of multiplexed loci-specific FISH signals,
described herein, is achieved through a fully automated, multicolour fluorescence imaging microscope and object segmentation algorithms to identify cell nuclei and FISH spots within. Related tumour clones are identified through analysis of robustly defined cell neighbourhoods and cell-to-cell connections for regions of cells with homogenous
and highly interconnected FISH spot signal characteristics.
This study presents experiments which demonstrate the system’s ability to
accurately quantify FISH spot signals in various tumour tissues and in up to 5 colours
simultaneously or more through multiple rounds of FISH staining. Furthermore, the
system’s FISH-based cell classification performance is evaluated at a sensitivity of 84% and specificity 81% and clonal identification algorithm results are determined to be comparable to clone delineation by a human-observer. Additionally, guidelines and procedures to perform anticipated, routine analysis experiments are established.
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Parrilla, Huertas Juan. "Espace géographique et environnement numérique : implications de l'hybridation fidigitale dans la praxis de la Géographie Humaine." Electronic Thesis or Diss., Pau, 2024. http://www.theses.fr/2024PAUU1134.
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La numérisation a déclenché une révolution dans l'étude de la Géographie, marquant un tournant décisif. La nécessité de comprendre les relations complexes entre la géographie et les technologies numériques devient de plus en plus évidente. Les nouvelles technologies ont transformé l'espace géographique, affectant en particulier la Géographie Humaine. L'avancement des Technologies de l'Information et de la Communication (TIC) a placé le numérique au centre de l'attention, devenant, comme l'indique Gillian Rose, à la fois objet et sujet de recherche, modifiant ainsi la pratique géographique et déclenchant un débat continu autour du "virage numérique".Ce virage a fourni de nouveaux outils à la Géographie pour relever les défis contemporains, transformant notre conception de l'espace en une entité qui fusionne des éléments tangibles et intangibles. La numérisation a créé des réalités hybrides où le physique et le numérique se mêlent, se connectent et se floutent, remettant en question les notions traditionnelles de territorialité et d'échelle. Cela oblige à revoir et à mettre à jour les approches géographiques, en développant de nouvelles méthodologies pour comprendre ces territoires hybrides et comment les étudier.La numérisation a ouvert de nombreuses possibilités et interrogations pour la science géographique, qui nécessitent des réponses d'une perspective intégrale et collaborative, en intégrant plusieurs disciplines. Tout d'abord, une étude exhaustive de l'état de l'art est réalisée, justifiant l'inclusion des concepts classiques de la Géographie et leur évolution dans le cadre des révolutions méthodologiques actuelles. Une "Généalogie des Géographies Numériques" est présentée, différenciant les concepts techniques liés à l'espace numérique et réfléchissant sur l'hybridation entre les espaces physique et numérique, ainsi que la redéfinition du rôle de la distance dans ce nouveau contexte.La thèse se concentre également sur l'application pratique de quatre études de cas de Géographie Humaine dans des environnements numériques, montrant comment le "virage numérique" a impacté la discipline. Ces recherches sont classées selon une taxonomie tripartite qui couvre les géographies générées, produites et propres au numérique. La Géographie Quantitative est reconnue comme précurseur de ces nouvelles formes d'analyse spatiale, adoptant des technologies numériques pour explorer l'espace.L'espace numérique est présenté comme une extension de l'espace relationnel, soulignant l'importance de la dualité spatiale et la redéfinition du concept de distance à l'ère numérique. L'immersion dans le numérique peut conduire à une fusion totale entre les espaces physique et numérique, où les individus expérimentent une perception sensorielle complète du lieu numérique. En s'intégrant dans les éléments numériques, les individus acquièrent un sentiment d'identité et d'appartenance, interagissent socialement, établissent des relations et ressentent des émotions dans cet espace hybrideDigitalization has triggered a revolution in the study of Geography, marking a decisive turning point. The need to understand the complex relationships between geography and digital technologies is increasingly evident. New technologies have transformed geographical space, particularly affecting Human Geography. The advancement of Information and Communication Technologies (ICT) has placed digital technology at the forefront, becoming, as Gillian Rose points out, both the object and subject of research, thus altering geographical practice and sparking a continuous debate around the "digital turn."This turn has provided Geography with new tools to address contemporary challenges, transforming our conception of space into an entity that merges tangible and intangible elements. Digitalization has created hybrid realities where the physical and digital intertwine, connect, and blur, challenging traditional notions of territoriality and scale. This necessitates a revision and update of geographical approaches, developing new methodologies to understand these hybrid territories and how to study them.Digitalization has opened many possibilities and questions for geographical science, requiring responses from an integral and collaborative perspective, incorporating multiple disciplines. Firstly, an exhaustive study of the state of the art is conducted, justifying the inclusion of classical geographical concepts and their evolution within the framework of current methodological revolutions. A "Genealogy of Digital Geographies" is presented, differentiating technical concepts related to digital space and reflecting on the hybridization between physical and digital spaces, as well as the redefinition of the role of distance in this new context. The thesis also focuses on the practical application of four case studies in Human Geography in digital environments, showing how the "digital turn" has impacted the discipline. These studies are classified according to a tripartite taxonomy that covers geographies generated, produced, and native to the digital realm. Quantitative Geography is recognized as a precursor to these new forms of spatial analysis, adopting digital technologies to explore space.Digital space is presented as an extension of relational space, highlighting the importance of spatial duality and the redefinition of the concept of distance in the digital age. Immersion in the digital realm can lead to a complete fusion between physical and digital spaces, where individuals experience a full sensory perception of the digital place. By integrating into digital elements, individuals gain a sense of identity and belonging, interact socially, establish relationships, and experience emotions within this hybrid space
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Ficele, Giovanni. "Distribution of proopiocortin (POC) and proopiomelanotropin (POM) mRNA during development of the sea lamprey, a quantitative spatial and temporal in situ hybridization study." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0001/MQ30063.pdf.
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Deymier, Nicolas. "Étude d’une méthode d’éléments finis d’ordre élevé et de son hybridation avec d’autres méthodes numériques pour la simulation électromagnétique instationnaire dans un contexte industriel." Thesis, Toulouse, ISAE, 2016. http://www.theses.fr/2016ESAE0038/document.
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Dans cette thèse, nous nous intéressons à l’amélioration du schéma de Yee pour traiter demanière plus efficace et pertinente les problèmes industriels auxquels nous sommes confrontés à l’heureactuelle. Pour cela, nous cherchons avant tout à diminuer les erreurs numériques de dispersion et àaméliorer les modélisations des géométries courbes ainsi que des réseaux de câbles. Pour répondre àces besoins, une solution basée sur un schéma Galerkin discontinu pourrait être envisagée. Toutefois,l’utilisation d’une telle technique sur la totalité du volume de calcul est relativement coûteuse. De plus,la prise en compte de structures filaires sur un tel schéma n’est pas encore opérationnelle. C’est pourquoi,dans l’optique d’avoir un outil industriel, et après une étude bibliographique, nous nous sommes plutôtorientés sur l’étude d’un schéma éléments finis (FEM) sur maillage cartésien qui possède toutes lesbonnes propriétés du schéma de Yee. Notamment, à l’ordre d’approximation spatiale égal à 0 ce schémaFEM est exactement le schéma de Yee, et, pour des ordres supérieurs, il permet de réduire fortementl’erreur de dispersion numérique de ce dernier. Dans le travail de cette thèse, pour ce schéma, nous avons notamment donné un critère de stabilité théorique, étudié sa convergence théorique et fait une analyse de l’erreur de dispersion. Pour tenircompte des possibilités d’ordre d’approximation spatiale variable par direction, nous avons mis en placeune stratégie d’affectation des ordres suivant le maillage donné. Ceci nous a permis d’obtenir un pas detemps optimal pour une précision souhaitée tout en réduisant les coûts de calcul. Après avoir porté ceschéma sur des machines de production, différents problèmes de CEM, antennes, IEM ou foudre ont ététraités afin de montrer les avantages et le potentiel de celui-ci. En conclusion de ces expérimentationsnumériques, il s’avère que la méthode est limitée par le manque de précision pour prendre en comptedes géométries courbes. Afin d’améliorer cela, nous avons proposé une hybridation entre ce schéma et leschéma GD que l’on peut étendre aux autres schémas comme les méthodes différences finies (FDTD) etvolumes finis (FVTD). Nous avons montré que la technique d’hybridation proposée conserve l’énergie etest stable sous une condition que nous avons évaluée de manière théorique. Des exemples de validationont ensuite été montrés. Enfin, pour tenir compte des réseaux de câbles, un modèle de fils minces d’ordred’approximation spatiale élevé a été proposé. Malheureusement, celui-ci ne peut pas couvrir l’ensembledes cas industriels et pour remédier à cela, nous avons proposé une hybridation de notre approche avecune équation de ligne de transmission. L’intérêt de cette hybridation a été montré sur un certain nombred’exemples, que nous n’aurions pas pu traiter par un modèle de structure filaire simpleIn this thesis, we study the improvement of the Yee’s scheme to treat efficiently and in arelevant way the industrial issues we are facing nowadays. For that, we first of all try to reduce thenumerical errors of dispersion and then to improve the modeling of the curved surfaces and of theharness networks. To answer these needs, a solution based on a Galerkin Discontinuous (GD) methodhas been first considered. However, the use of such method on the entire modeling volume is quite costly ;moreover the wires are not taken into account in this method. That is the reason why, with the objectiveof an industrial tool and after a large bibliographic research, we headed for the study of finite elementsscheme (FEM) on a Cartesian mesh which has all the good properties of the Yee’s scheme. Especially,this scheme is exactly the Yee’s scheme when the spatial order of approximation is set to zero. Forthe higher orders, this new scheme allows to greatly reduce the numerical error of dispersion. In theframe of this thesis and for this scheme, we give a theoretical criterion of stability, study its theoreticalconvergence and we perform an analysis of the error of dispersion. To take into account the possibilityof the variable spatial orders of approximation in each direction, we put in place a strategy of orderaffectation according to the given mesh. This strategy allows to obtain an optimal time step for a givenselected precision while reducing the cost of the calculations. Once this new scheme has been adaptedto large industrial computing means, different EMC, antennas, NEMP or lightning problems are treatedto demonstrate the advantages and the potential of this scheme. As a conclusion of these numericalsimulations we demonstrate that this method is limited by a lack of precision when taking into accountcurved geometries. To improve the treatment of the curved surfaces, we propose an hybridization between this scheme andthe GD scheme. This hybridization can also be applied to other methods such as Finite Differences(FDTD) or Finite Volumes (FVTD). We demonstrate that the technique of hybridization proposed,allows to conserve the energy and is stable under a condition that we study theoretically. Some examplesare presented for validation. Finally and to take into account the cables, a thin wire model with a highorder of spatial approximation is proposed. Unfortunately, this model does not allow to cover all theindustrial cases. To solve this issue we propose an hybridization with a transmission line method. Theadvantage of this hybridization is demonstrated thanks to different cases which would not have beenfeasible with a more simple thin wire method
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Rönsch, Kathleen. "Patterning of stem cells during limb regeneration in Ambystoma mexicanum." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-232386.
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Axolotl uniquely generates blastema cells as a pool of progenitor/stem cells to restore an entire limb, a particular property that other organisms, such as humans, do not have. What underlies these differences? Is the main difference that cells residing at the amputation plane (in the stump) undergo reprogramming processes to re-enter the embryonic program, which allows developmental patterning to start, or are there fundamental differences? There is also a significant debate about whether regeneration occurs via stem cell differentiation or by dedifferentiation of mature limb tissue. The aim of my thesis was to address following questions: Are the cells in the blastema reprogrammed or differentiated to regenerate? Are the blastema cells genetically reactivated de novo during regeneration? How does the amputated limb exactly know which part of the limb needs to be regenerate?
Using a novel technique of long-term genetic fate mapping, my team demonstrated that dedifferentiation in regenerated axolotl muscle tissue does not occur. Instead, PAX7+ satellite cells indeed play an important role during muscle regeneration in the axolotl limb. Surprisingly, this is in contrast to the newt, which regenerates muscle cells through a dedifferentiation process. Therefore, there is a fundamental difference that underlies the regenerative mechanism ((Sandoval-Guzman et al., 2014) [KR1]). This demonstrates that there is an unexpected diversity and flexibility of cellular mechanims used during limb regeneration, even among two closely related species. Finally, if one salamander species uses a mammalian regenerative strategy (Cornelison and Wold, 1997; Collins et al., 2005) involving stem cells and another uses a dedifferentiative strategy, this raises the question of whether there are other fundamental aspects of regeneration that could also be anomalous. This hypothesis is promising since there could be more than one possible mechanism to induce mammalian regeneration.
The process of limb regeneration in principle seems to be more similar to those of limb development as historically assumed. We showed molecularly that embryonic players are reused during regeneration by reactivating the position- and tissue-specific developmental gene programs by using the newly isolated Twist sequences as early blastema cell markers ((Kragl et al., 2013) [KR2]). To gain insights into the molecular mechanisms of the P/D limb patterning in general, it was crucial to study the early patterning events of the resident progenitor/stem cells by using the specific blastema cell marker HoxA as a positional marker along the proximo-distal axis. Our HOXA protein analysis using high molecular and cellular resolution as well as transplantation assays demonstrated for the first time that axolotl limb blastema cells acquire their positional identity in a proximal to distal sequence. We found a hierarchy of cellular restrictions in positional identities. Amputation at the level of the upper arm showed that the blastema harbors cells, which convert to lower arm and hand. We observed ((Roensch et al., 2013) [KR3]) for the first time that intercalation- the intermediate element (lower arm) arises later from an interaction between the proximal and distal cells identities- does not occur. Intercalation, which has been an accepted model for a long time, is not the patterning mechanism underlying normal (without any manipulation) limb regeneration that is unique to axolotl. We further demonstrated, using the Hox genes as markers that positional identity is cell-type specific since their effects were confirmed to be present in the lateral plate mesoderm- derived cells of the limb.
As our knowledge about limb blastemas expands concerning cell composition and molecular events controlling patterning, the similarity to development is becoming more and more clear. My work has resolved many ambiguities surrounding the molecularly identification of different types of blastema cells and how P/D limb patterning occurs during regeneration in comparison to development. It has highlighted the importance of combining high-resolution methods, such as in situ hybridizations, single-cell PCR (sc-PCR) of individual dissociated blastema cells and genetic labeling methods with grafting experiments to map cell fates in vivo.
In addition to understanding the processes of regeneration, another long-term goal in the regenerative medicine field is to identify key molecules that trigger the regeneration of tissues. Recently, my colleague Takuji Sugiura (Sugiura et al., 2016) observed that an early event of blastema formation is the secretion of molecules like MLP (MARCKS-like protein), which induces wound-associated cell cycle re-entry. Such findings further increase the enthusiasm of biologists to understand the underlying principles of regeneration. By building our knowledge of the molecules and pathways that are involved in tissue regeneration, we increase the possibility of identifying a way to ‘activate’ regenerative processes in humans and thus reach the final goal of regenerative medicine, which is to use the concepts of cellular reprogramming, stem cell biology and tissue engineering to repair complex body structures.
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Colas, Steven. "Expression spatio-temporelle de deux protéines PR du grain de raisin - dégradation au cours de l'infection par Botrytis cinerea -." Thesis, Reims, 2012. http://www.theses.fr/2012REIMS024/document.
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L'infection des baies de raisin par le champignon Botrytis cinerea, responsable de la pourriture grise, est fréquente et occasionne des dégâts importants. Pourtant, il semble que la baie dispose de moyens de défense parmi lesquels des protéines PR "Pathogenesis-Related". Chez le Pinot Noir, une chitinase (CHI4D) et une thaumatin-like (TL3) s'accumulent naturellement en grande quantité à partir de la véraison et présentent une activité antifongique contre B. cinerea in vitro. L'objectif de ce travail est de comprendre comment B. cinerea peut se développer sur des baies censées disposer de défenses suffisantes. Pour cela, l'expression spatio-temporelle des ARNm et des protéines CHI4D et TL3 a été suivie respectivement par hybridation in situ et immunohistolocalisation dans les baies, au cours de la maturation, à la suite d'un stress abiotique (UV-C) ou d'un stress biotique (B. cinerea). Dans des baies avant véraison (vertes), n'exprimant naturellement que très faiblement CHI4D et TL3, les ARNm et les protéines s'accumulent en grande quantité après application d'un stress abiotique (UV-C) ou biotique (infection artificielle par B. cinerea). Les protéines CHI4D et TL3 sont localisées au niveau des faisceaux conducteurs ainsi qu'au niveau des tissus proches des sites d'exposition aux UV-C (exocarpe) ou au niveau des sites d'inoculation de B. cinerea, suggérant qu'elles sont impliquées dans la défense de la baie avant véraison. Après véraison, les ARNm et les protéines sont naturellement accumulés au niveau de l'exocarpe et des faisceaux conducteurs qui correspondent à des sites potentiels d'entrée ou de propagation des agents pathogènes. Alors que l'application d'un stress UV-C sur ces baies ne provoque qu'un effet mineur sur l'expression de CHI4D et de TL3, au cours de l'infection par B. cinerea, les quantités de transcrits et de protéines diminuent. A un stade précoce d'infection, la diminution de la quantité des deux protéines est observée en avant du front de propagation du champignon, suggérant une dégradation par des protéases sécrétées par B. cinerea. A un stade d'infection plus avancé, cette diminution s'étend à l'ensemble de la baie. La production hétérologue des protéines CHI4D et TL3 nous a permis de confirmer que CHI4D pouvait être dégradée par des protéases aspartiques sécrétées par B. cinerea. La dégradation de TL3 n'a pas pu être reproduite in vitro. Des tests antigerminatifs effectués in vitro avec les protéines hétérologues n'ont pas permis de mettre en évidence d'effet antifongique malgré la présence d'une activité chitinase pour CHI4D et β-1,3,-glucanase pour TL3. Il est donc possible que ces protéines possèdent des fonctions autres que celles impliquées dans la défenseGrape berries infection by the phytopathogenic fungus Botrytis cinerea, the causal agent of gray mold, is quite common and causes significant damage. However, it seems that berries have a mechanism of defense, among which are pathogenesis related proteins. In Pinot Noir grape berries, a chitinase (CHI4D) and a thaumatin-like (TL3) protein naturally accrue in large amounts from véraison and show in vitro an antifungal effect against B. cinerea. The aim of this work was to understand how B. cinerea can develop on grape berries that seem to have sufficient defense mechanisms. To do so, the spatio-temporal expression of CHI4D and TL3 mRNAs and proteins in berries was studied respectively by in situ hybridization and immunohistolocalization during maturation, after an abiotic stress (UV-C) or a biotic stress (B. cinerea). Before véraison (green berries) while the expression of CHI4D and TL3 is naturally low, mRNAs and proteins have accumulated in large amounts in berries after UV-C exposition or artificial infection with B. cinerea. CHI4D and TL3 proteins have accumulated around vascular bundles as well as near the sites of UV-C exposition (exocarp) or B. cinerea inoculation, suggesting that before véraison these proteins could be involved in the berry defense. After veraison, mRNAs and proteins naturally accumulate in the exocarp and around vascular bundles that correspond to potential sites of penetration or propagation of pathogenic agents. While the application of UV-C stress on these berries causes only a minor effect on the expression of CHI4D and TL3, during infection by B. cinerea, the amounts of mRNA and proteins decreased. At an early stage of infection, the less amounts of both proteins were observed around the fungus propagation area, suggesting that these proteins could be degraded by B. cinerea secreted proteases. At a more advanced stage of infection, the decrease extended to the entire berry.Production of heterologous CHI4D and TL3 proteins allowed us to confirm that CHI4D could be degraded by aspartic proteases secreted by B. cinerea whereas no degradation of TL3 could be observed in vitro. Both heterologous proteins showed no antifungal effect while a chitinase and a β-1,3-glucanase activities were observed respectively for CHI4D and TL3. It is therefore possible that these proteins have other functions than those involved in the defense
Books on the topic "Spatial hybridization"
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Michele, Luminati. Costruire, trasformare, controllare. Edizioni Casagrande, 2022. http://dx.doi.org/10.35263/casagrande-951-1.
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The essays collected in this volume draw from the history of law and institutions, history of art and urban planning, geography and cartography, economic and social history, and propose a reflection on center-periphery relations, the hybridization between cultures, and the adaptation of “external” models in the definition, construction and control of urban, rural and state space. The subject of this interdisciplinary investigation is mainly the city of Milan, the Lombard region and the area of Canton Ticino between the end of the eighteenth century and the first decades of the nineteenth century: a period marked by important political-legal transitions, during which on the one hand the efforts of state authorities were aimed at implementing new legislative references for spatial and territorial organization, and on the other hand phenomena of resistance and opposition to this new legislation emerged.
Book chapters on the topic "Spatial hybridization"
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Freger, G. E., V. N. Kestelman, and D. G. Freger. "Hybridization and Spatial Reinforcement of Composite Materials." In Spirally Anisotropic Composites, 1–26. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-662-09975-9_1.
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Miya, Masayuki, Ken-ichiro Hibara, and Jun-ichi Itoh. "Spatial Analysis of Gene Expression by In Situ Hybridization." In Methods in Molecular Biology, 49–59. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-4204-7_7.
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Traylor-Knowles, Nikki, and Madison Emery. "Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals." In Methods in Molecular Biology, 359–71. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2172-1_19.
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AbstractScleractinians, or stony corals, are colonial animals that possess a high regenerative capacity and a highly diverse innate immune system. As such they present the opportunity to investigate the interconnection between regeneration and immunity in a colonial animal. Understanding the relationship between regeneration and immunity in stony corals is of further interest as it has major implications for coral reef health. One method for understanding the role of innate immunity in scleractinian regeneration is in situ hybridization using RNA probes. Here we describe a protocol for in situ hybridization in adult stony corals using a digoxigenin (DIG)-labeled RNA antisense probe which can be utilized to investigate the spatial expression of immune factors during regeneration.
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Clements, Craig S., Ural Bikkul, Mai Hassan Ahmed, Helen A. Foster, Lauren S. Godwin, and Joanna M. Bridger. "Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence In Situ Hybridization." In Methods in Molecular Biology, 387–406. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3530-7_24.
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Roberts, Kenny, and Omer Ali Bayraktar. "Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping." In Methods in Molecular Biology, 229–44. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0623-0_15.
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Miles, Sam. "Let’s (not) Go Outside: Grindr, Hybrid Space, and Digital Queer Neighborhoods." In The Life and Afterlife of Gay Neighborhoods, 203–20. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-66073-4_9.
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AbstractDevelopments in mobile digital technologies are disrupting conventional understandings of space and place for smartphone users. One way in which location-based media are refiguring previously taken-for-granted spatial traditions is via GPS-enabled online dating and hook-up apps. For sexual minorities, these apps can reconfigure any street, park, bar, or home into a queer space through a potential meeting between mutually attracted individuals, but what does this signify for already-existing queer spaces? This chapter examines how smartphone apps including Grindr, Tinder, and Blued synthesize online queer encounter with offline physical space to create a new hybrid terrain predicated on availability, connection, and encounter. It is also a terrain that can sidestep established gay neighborhoods entirely. I explore how this hybridization impacts on older, physically rooted gay neighborhoods and the role that these neighborhoods have traditionally played in brokering social and sexual connection for sexual minorities. Few would deny that location-based apps have come to play a valuable role in multiplying opportunities for sexual minorities. However, the stratospheric rise of these technologies also provokes questions about their impact on embodied encounter, queer community, and a sense of place. A decade on from Grindr’s release, this chapter evaluates the impact of location-based media on gay spaces and reflects on what the increasing hybridization of online and offline spaces for same-sex encounter might mean for queer lives of the future.
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Ciaffi, Daniela, Carolina Giaimo, Emanuela Saporito, and Valeria Vitulano. "Space for Rights. The School Between Planning Standard and Social Innovation." In The Urban Book Series, 141–50. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-33894-6_11.
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AbstractThe paper straddles the boundary between urbanism and sociology, working on the common ground of rights: public services and facilities (‘planning standard,’ according to the Italian spatial planning legislation) on the one hand and the care of the commons on the other, in addition to attempting to grapple with a third dimension of rights that places space and society alongside law. This methodological hypothesis is practiced from the critical analysis of one of the basic public facilities: the school. In the national debate on public services and common goods, school spaces are one of the recurring examples of how they have functionality as public educational services during school time and how they can also have other functional profiles as common goods, i.e., as civic centers open to the urban community during out-of-school hours. The theme of the hybridization of spaces and functions emerges with ever-increasing theoretical and empirical force in the reflections on so-called social innovation. Even if, in several cases, many people ignored one of the most beautiful definitions of planning standards by Giovanni Astengo (1966). He stated that, besides being a minimum, standards represent a minimum of civilization. The paper intends to bring attention to the complex value of spatial and social resources related to schools.
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Gates, Ruth D., Thorsten Hadrys, Cesar Arenas-Mena, and David K. Jacobs. "Determining the Spatial and Temporal Patterns of Developmental Gene Expression in Vertebrates and Invertebrates Using in situ Hybridization Techniques." In Techniques in Molecular Systematics and Evolution, 365–94. Basel: Birkhäuser Basel, 2002. http://dx.doi.org/10.1007/978-3-0348-8125-8_17.
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Bradbury, Joshua J., Holly E. Lovegrove, Marta Giralt-Pujol, and Shane P. Herbert. "Analysis of mRNA Subcellular Distribution in Collective Cell Migration." In Cell Migration in Three Dimensions, 389–407. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_22.
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AbstractThe movement of groups of cells by collective cell migration requires division of labor between group members. Therefore, distinct cell identities, unique cell behaviors, and specific cellular roles are acquired by cells undergoing collective movement. A key driving force behind the acquisition of discrete cell states is the precise control of where, when, and how genes are expressed, both at the subcellular and supracellular level. Unraveling the mechanisms underpinning the spatiotemporal control of gene expression in collective cell migration requires not only suitable experimental models but also high-resolution imaging of messenger RNA and protein localization during this process. In recent times, the highly stereotyped growth of new blood vessels by sprouting angiogenesis has become a paradigm for understanding collective cell migration, and consequently this has led to the development of numerous user-friendly in vitro models of angiogenesis. In parallel, single-molecule fluorescent in situ hybridization (smFISH) has come to the fore as a powerful technique that allows quantification of both RNA number and RNA spatial distribution in cells and tissues. Moreover, smFISH can be combined with immunofluorescence to understand the precise interrelationship between RNA and protein distribution. Here, we describe methods for use of smFISH and immunofluorescence microscopy in in vitro angiogenesis models to enable the investigation of RNA and protein expression and localization during endothelial collective cell migration.
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Allkja, Jontana, and Andreia S. Azevedo. "Characterization of Social Interactions and Spatial Arrangement of Individual Bacteria in MultiStrain or Multispecies Biofilm Systems Using Nucleic Acid Mimics-Fluorescence In Situ Hybridization." In Methods in Molecular Biology, 97–109. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1115-9_7.
Full textConference papers on the topic "Spatial hybridization"
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Malyutin, Oleg, and Renat Khabibulin. "Modular Approach to Hybridization of Heuristic Algorithms for Spatial Optimization of Fire Units Placement." In 2024 6th International Conference on Control Systems, Mathematical Modeling, Automation and Energy Efficiency (SUMMA), 837–40. IEEE, 2024. https://doi.org/10.1109/summa64428.2024.10803695.
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Stojković, Marija. "HYBRIDIZATION OF PUBLIC CITY SPACES AS A TOOL FOR SUSTAINABLE URBAN REGENERATION." In 20th SCIENTIFIC-PROFESSIONAL CONFERENCE WITH INTERNATIONAL PARTICIPATION “URBANISM AND SUSTAINABLE DEVELOPMENT”. Serbian Town Planner Association, 2024. http://dx.doi.org/10.46793/urbanizam24.113s.
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In recent years, we have witnessed an increasingly pronounced tendency to combine and mix different urban structures, programs, and activities that create hybrid urban spaces. Hybridization as a method implies a new relationship between the content and the elements of the space, which increases its multifunctionality, flexibility and potentially expands the range of uses. Therefore, in urban theory and practice, it is recognized as a possible catalyst for the creation of sustainable urban environments. However, the existing theoretical discourses shed light on certain dimensions of spatial hybridization but do not enable its complex and comprehensive understanding in relation to specific aspects of the contribution of public spaces to sustainable urban development. In this sense, this paper aims to identify and define different dimensions/aspects of hybridization of public urban spaces and to consider and discuss the contribution of specific aspects of hybridization to ecological, economic, and social sustainability using selected examples (High Line and La Villette). The relationship between the city and nature and the positive regenerative effect that the process of hybridization of public spaces can have on the wider urban area, including the city itself, are reexamined. The research results suggest that hybrid spaces have regenerative power, establish new micro and macro spatial relationships, are important for economic development, and can be initiators of significant social changes.
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Cortese, Erika, Joshua Mornhinweg, Christoph Lange, and Simone De Liberato. "Real-space nanophotonic field manipulation using Landau polaritons." In CLEO: Applications and Technology. Washington, D.C.: Optica Publishing Group, 2022. http://dx.doi.org/10.1364/cleo_at.2022.jw3b.164.
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We develop the theory of multimode hybridization in polariton platforms. In the specific case of Landau polaritons we demonstrate the possibility of tuning the near-field electromagnetic spatial mode profile by hybridising multiple metamaterial modes.
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Skogberg, Gabriel, Sonya Jackson, Britt-Marie Fihn, Ulrika Tehler, Johan Karlsson, Michael Hühn, and Markus Fridén. "RNAscope in situ hybridization allows spatial estimation of free drug exposure through quantification of drug induced gene expression." In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa3377.
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Ismael, Yaseen. "Secure Image Steganography by Utilizing DNA Properties." In 3rd International Conference of Mathematics and its Applications. Salahaddin University-Erbil, 2020. http://dx.doi.org/10.31972/ticma22.08.
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In the last period, Steganography is commonly used as an alternative to encryption to achieve secret communication between parties. Many methods have emerged to achieve steganography, including the use of spatial domain, spread spectrum, transform domain, and etc. On the other hand, the methods of attackers have also developed in revealing hidden data and trying to retrieve it. To increase the security of the hiding process, some researchers have found hybrid methods that combine encryption and steganography processes. The research aims to present a new method in steganography by taking advantage of the properties of DNA, which includes the random sequence of nitrogenous bases (A, C, G, T), the process of hybridization, which occurs between two single strands of DNA to form a double strand of DNA so that the bases in the first strand are complementary to the nitrogenous bases in the second strand. The research includes the following steps: First, the secret image to be hidden is encrypted by encoding it into a series of nitrogenous bases, and then the XOR process is performed with a nitrogenous bases sequence for a DNA tape agreed upon between the sender and recipient, the hybridization process applied before and after the XOR process. The results show that encrypted image is much different from the original image and thus they added another level of security to the hidden image. Secondly, the encrypted image resulting from the first step is hidden in the cover image and using a new method based on the use of the agreed-upon DNA tape as a key.
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Li, Jingyao, Dongdong Lin, and Yu-Ping Wang. "Segmentation of Multicolor Fluorescence In-Situ Hybridization (M-FISH) image using an improved Fuzzy C-means clustering algorithm while incorporating both spatial and spectral information." In 2015 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2015. http://dx.doi.org/10.1109/bibm.2015.7359717.
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Stock, Mark J., and Adrin Gharakhani. "A Hybrid High-Order Vorticity-Based Eulerian and Lagrangian Vortex Particle Method, the 2-D Case." In ASME 2021 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2021. http://dx.doi.org/10.1115/fedsm2021-65637.
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Abstract Hybrid Lagrangian-Eulerian solvers combine the convective and compactness advantages of vortex methods with the spatial anisotropy and boundary-resolving advantages of Eulerian methods to create flexible solvers capable of adequately capturing thin boundary layers while still maintaining wake vortex coherency for unsteady incompressible flow in complex geometries. The present paper details a new hybrid method which combines, in one open-source package, a novel, compact, high-order Eulerian scheme for vorticity transport to predict the flow in the near-boundary region with a grid-free, unremeshed, Lagrangian Vortex Particle Method (LVPM) for the off-boundary vorticity-containing region. This paper focuses on the hybridization of the two methods and demonstrates its effectiveness on two canonical benchmarks: flow in 2-D lid-driven cavity at Re = 1,000 and impulsively started flow over a circular cylinder at Re = 9,500. In each case, the hybrid method improves upon a pure LVPM and uses far fewer cells than a purely Eulerian scheme. In addition, the size of the associated Eulerian region is greatly reduced compared to previous hybrid methods.
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Occhiuto, Rita. "Resistance & Permanence of Green Urban Systems in the Globalization Age." In 24th ISUF 2017 - City and Territory in the Globalization Age. Valencia: Universitat Politècnica València, 2017. http://dx.doi.org/10.4995/isuf2017.2017.6328.
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Rita Occhiuto Faculté d’Architecture. Université de Liège, ULG. 1, Rue Courtois 4000 Liège (BE) Tél. +3242217900 e-mail : r.occhiuto@ulg.ac.be Keywords: public space, park system, green and water infrastructure, morphological green writings, landscape memory The rapid transformation and the trivialization of landscapes in Wallonia (BE), require reformulating tools and objectives of morphological studies. Built fabrics and landscapes show the effects of abandoning or losing interest in the interrelations between natural and human actions. This contribution focuses on studies of cities and territories that have ceased to be the object of spatial policies attentive to the relationship between the need to live, maintain or care for green or natural spaces. After the systematic reduction of urban environments to simple green covers, morphological reading allows the recognition of traces of park systems or green infrastructures, whose communities often do not remember. The research's focus has shifted from the building to the green space structure. This displacement of interest makes it possible to find commons cultures that have acted on the territory of Liège (industrial city) on the one hand, through the building’s extension and on the other hand, through the project of forests, walks, squares, parks and public gardens. Now, these fragmented places become the main resource for reorganizing natural and human systems in order to offer new - social and spatial - coherence for tomorrow. Thus the historical green systems become a strong structuring link which serves to seek new dialectics of balance between existing fabrics and green systems. This system’s regeneration stands, on the one hand, to the hybridization of materials - water, green and buildings - and, on the other hand, to the physical and mental memory of the inhabited environments that populations keep. Green systems impose themselves as powerful vectors for the construction of new socio-spatial balances of cities and territories of globalization, as in the study case for the landscape systems in Liège and for the water and landscapes infrastructure in Chaudfontaine.References Foxley, A. (2010), Distance & engagement. Walking, thinking and making landscape. Vogt landscape architects, Lars Müller Publishers Cronon,W., Coll., Uncommon ground. Rethinking the Human Place in Nature. W.W.Norton & Company New York/London McHarg, I.(1969), Design with Nature, 1th, New York Spirn, A.W. (1994), The granite garden. Urban Nature and Human Design, ed. Basic Book Ravagnati, C. (2012), L’invenzione del Territorio. L’atlante inedito di Saverio Muratori, ed. Franco Angeli, Milano
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Oyinna, Benneth Chimaobi, and Michael O. Ukoba. "Geographical Information System Based Assessment of Small Hydropower Potential in South-Eastern Nigeria: A Case Study of Abia State." In Africa International Conference on Clean Energy and Energy Storage. Switzerland: Trans Tech Publications Ltd, 2024. http://dx.doi.org/10.4028/p-a0roum.
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Small hydropower technology has gained traction in the Nigerian energy and power ecosystem owing to incentives and reforms aimed at increasing Nigeria’s energy mix for sustainable development. Utilizing these opportunities through harnessing SHP potentials has not made it to the front burner during policy formulations and implementations in South-Eastern Nigeria despite the availability of water bodies and waterlines in the region. This paper focuses on the potentials of small hydropower in Abia state and utilized ArcGIS software to conduct spatial analysis using map data overlayed by shapefiles of water bodies, waterlines, road networks and Land use, Land Cover data (LULC). Multiple ring buffers were created for various proximities around the waterbodies and waterlines and Normalized Difference Vegetation Index calculations were done to determine suitability areas for small hydropower schemes after reclassification of the data. The analysis revealed suitability areas in Osisioma Ngwa and Obingwa Local Government areas with suitable elevations and hydraulics data for run off the river schemes and siting of hydropower plants within a multiple ring buffer distance between 200m to 5km from the waterlines and roads, having a weighted score between 33-66 with NDVI range of -0.018 –0.015 indicating the presence of water bodies and built-up areas around the water bodies with NDVI range of 0.015 – 0.14 and a weighted score within the range of 11-16 This revelation also encourages the hybridization of renewable energy technology using pumped hydro storage to improve the reliability and affordability of mini-grid solutions in Abia State and Nigeria at large.
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Coleski, Andrej, Sethu Pitchiaya, Jeremy D'Silva, Marcin Cieslik, and Arul Chinnaiyan. "Abstract 2162: Spatially resolved image-based transcriptomics using high-throughput single-molecule fluorescence in situ hybridization (HITSFISH)." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-2162.
Full textReports on the topic "Spatial hybridization"
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Weiss, Gregory, and Aidan Thompson. Spatially-dependent Hybridization of Potentials in LAMMPS. Office of Scientific and Technical Information (OSTI), April 2018. http://dx.doi.org/10.2172/1488473.
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