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Journal articles on the topic "SP-ELISA"
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CIMINO, R. O., M. MONJE RUMI, P. RAGONE, J. LAUTHIER, A. ALBERTI D'AMATO, I. R. LÓPEZ QUIROGA, J. F. GIL, et al. "Immuno-enzymatic evaluation of the recombinant TSSA-II protein ofTrypanosoma cruziin dogs and human sera: a tool for epidemiological studies." Parasitology 138, no. 8 (April 26, 2011): 995–1002. http://dx.doi.org/10.1017/s0031182011000540.
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SUMMARYThe rTSSA-II (recombinant Trypomastigote Small Surface II) antigen was evaluated by ELISA to detect anti-Trypanosoma cruziantibodies in sera from naturally infected dogs and humans. For this evaluation ELISA-rTSSA-II was standardized and groups were classified according to the results obtained through xenodiagnosis, ELISA and PCR. Sensitivity (Se), Specificity (Sp), Kappa index (KI) and area under curve (AUC) were determined. The Se was determined by using 14 sera from dogs infected withT. cruziVI (TcVI) whereas Sp was determined by using 95 non-chagasic sera by xenodiagnosis, ELISA-Homogenate and PCR. The performance of ELISA-rTSSA-II in dog sera was high (AUC=0·93 and KI=0·91). The Se was 92·85% (1 false negative) and Sp was 100%. Two sera from dogs infected with TcI and 1 with TcIII were negative. For patients infected withT. cruzi, reactivity was 87·8% (36/41), there was only 1 indeterminate, and Sp was 100%. Fifty-four sera from non-chagasic and 68 sera from patients with cutaneous leishmaniasis did not react with rTSS-II. ELISA-rTSSA-II showed a high performance when studying sera from naturally infected dogs and it also presented 100% Sp. This assay could be an important tool to carry out sero-epidemiological surveys on the prevalence ofT. cruzicirculating lineages in the region.
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Ramírez-Reveco, Alfredo, Gerardo Velásquez, Christopher Aros, Gabriela Navarrete, Franz Villarroel-Espíndola, Maritza Navarrete, Alberto Fica, et al. "Performance estimation of two in-house ELISA assays for COVID-19 surveillance through the combined detection of anti-SARS-CoV-2 IgA, IgM, and IgG immunoglobulin isotypes." PLOS ONE 18, no. 2 (February 6, 2023): e0270388. http://dx.doi.org/10.1371/journal.pone.0270388.
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The main objective of this study was to estimate the performance, under local epidemiological conditions, of two in-house ELISA assays for the combined detection of anti-SARS-CoV-2 IgA, IgM, and IgG immunoglobulins. A total of 94 serum samples were used for the assessment, where 44 corresponded to sera collected before the pandemic (free of SARS-CoV-2 antibodies), and 50 sera were collected from confirmed COVID-19 patients admitted to the main public hospital in the city of Valdivia, southern Chile. The Nucleocapsid (Np) and the receptor-binding domain (RBD) proteins were separately used as antigens (Np and RBD ELISA, respectively) to assess their diagnostic performance. A receiver operating characteristic (ROC) analysis was performed to estimate the optical density (OD) cut-off that maximized the sensitivity (Se) and specificity (Sp) of the ELISA assays. Np ELISA had a mean Se of 94% (95% CI = 83.5–98.8%) and a mean Sp of 100% (95% CI = 92.0–100%), with an OD 450 nm positive cut-off value of 0.88. On the other hand, RBD ELISA presented a mean Se of 96% (95% CI = 86.3–99.5%) and a mean Sp of 90% (95% CI = 78.3–97.5%), with an OD 450 nm positive cut off value of 0.996. Non-significant differences were observed between the Se distributions of Np and RBD ELISAs, but the latter presented a significant lower Sp than Np ELISA. In parallel, collected sera were also analyzed using a commercial lateral flow chromatographic immunoassay (LFCI), to compare the performance of the in-house ELISA assays against a commercial test. The LFCI had a mean sensitivity of 94% (95% CI = 87.4–100%) and a mean specificity of 100% (95% CI = 100–100%). When compared to Np ELISA, non-significant differences were observed on the performance distributions. Conversely, RBD ELISA had a significant lower Sp than the LFCI. Although, Np ELISA presented a similar performance to the commercial test, this was 2.5 times cheaper than the LFCI assay (labor cost not considered). Thus, the in-house Np ELISA could be a suitable alternative tool, in resource limited environments, for the surveillance of SARS-CoV-2 infection, supporting further epidemiological studies.
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Wahyuningsih, Sri P. Astuti, R. Warsito, Hastari Wuryastuti, and Kamiso H.N. "DETEKSI Streptococcus Sp PADA Clarias gariepinus MENGGUNAKAN AMPLIFIED ENZYME-LINKED IMMUNOSORBENT ASSAY." Berkala Penelitian Hayati 5, no. 1 (December 31, 1999): 23. http://dx.doi.org/10.23869/bphjbr.5.1.19993.
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An enzyme-linked immunosorbent assay has been developed which detect Streptococcus sp. Twenty Clarian gariepinus at the age of two weeks was soaked in Streptococcus sp. suspension with concentration of 108 bacteria per ml for two hours. Specimens such as blood, mucous, muscles, heart, kidney, liver, intestines and gill were collected, and assayed for the presence of Steptococcus sp. using ELISA. Result of present study showed that ELISA can be applied to detect an isolated Streptococcus sp. from tissues of Clarias gariepinus. Kidney is a primary target organ (predilection). Therefore, the kidney is a specimen of choice for diagnosis approach (es) of Streptococcus sp. infection.
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Oliveira, Trícia Maria F. de Sousa, Patrícia I. Furuta, Débora de Carvalho, and Rosangela Z. Machado. "Study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and Ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test." Revista Brasileira de Parasitologia Veterinária 17, no. 1 (March 2008): 7–11. http://dx.doi.org/10.1590/s1984-29612008000100002.
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To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by Indirect Fluorescent Antibody (IFAT) and Enzyme-linked immunosorbent assay (ELISA). All serum samples from endemic areas were positive for Leishmania sp., by ELISA and IFAT, 51% positive for Babesia canis and 43% for Ehrlichia canis by IFAT. None of the serum samples from non-endemic areas were positive for Leishmania sp., by IFAT, but 67% were positive for B. canis and 78% for E. canis using the same test. When tested by ELISA for Leishmania sp., four samples from non-endemic area were positive. These dogs were then located and no clinical signs, parasites or antibody was detected in new tests for a six month period. Only one of these 4 samples was positive for B. canis by IFAT and ELISA and three for E. canis by IFAT. The results of the work suggest a co-infection in the endemic area and no serological cross-reaction among these parasites by IFAT and ELISA.
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METZGER-BODDIEN, CHRISTOPH, ANJA BOSTEL, and JOHANNES KEHLE. "AnDiaTec Salmonella sp. PCR-ELISA for Analysis of Food Samples." Journal of Food Protection 67, no. 8 (August 1, 2004): 1585–90. http://dx.doi.org/10.4315/0362-028x-67.8.1585.
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A commercially available PCR kit (AnDiaTec Salmonella sp. PCR-ELISA) was developed and evaluated for the detection of Salmonella sp. in food samples. The test is based on PCR amplification and hybridization of the amplified DNA to a microtiter plate followed by the detection of PCR product in the manner of an enzyme-linked immunosorbent assay test. The sensitivity and specificity were evaluated first with Salmonella pure cultures and artificially contaminated food samples, including food types for which an inhibition of the PCR reaction was expected. Both experiments proved a very good sensitivity, specificity, and reliability of the test with a very broad range of food types. In a second evaluation study, more than 1,100 food samples of different types were tested in parallel with the PCR method and with the International Standardization Organization 6579 bacteriological reference method. The results of this evaluation study and the results from other experiments on dilutions of artificially contaminated food samples led to the establishment of a positive-negative cutoff value (optical density at 450 nm of more than 0.9) with respect to the conventional bacteriological method. Using this positive-negative cutoff, 98% agreement to the bacteriological method was obtained.
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Garcia-de-Lomas, J., C. Morales, M. A. Grau, and A. Mir. "Detection of Candida sp. mannan antigen by indirect ELISA-inhibition." Mycopathologia 102, no. 3 (June 1988): 175–78. http://dx.doi.org/10.1007/bf00437401.
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Paré, Julie, Sharon K. Hietala, and Mark C. Thurmond. "An Enzyme-Linked Immunosorbent Assay (ELISA) for Serological Diagnosis of Neospora Sp. Infection in Cattle." Journal of Veterinary Diagnostic Investigation 7, no. 3 (July 1995): 352–59. http://dx.doi.org/10.1177/104063879500700310.
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A kinetic enzyme-linked immunosorbent assay (ELISA) was developed and optimized for detection of antibodies to Neospora sp. in cattle. Sonicated tachyzoites of Neospora sp. isolated from an aborted bovine fetus were used as antigen. Variability in immunoblot patterns among positive sera, and the fact that all life stages of the parasites are unknown, justified use of a multiple-antigen ELISA to allow for maximum sensitivity. Immunoblot analysis revealed negligible cross-reactions between Toxoplasma gondii antigen and Neospora sp. antisera and between Neospora sp. antigen and antisera from various apicomplexan parasites. The maximum positive-to-negative Vmax (average maximum slope of the optical density over time) ratio was obtained using 200 ng/well of sonicated tachyzoite antigen and a 1:200 serum dilution. Using logistic regression to determine the optimal cutoff point between known infected and noninfected cattle, a sample-to-positive control Vmax ratio of 0.45 was found to maximize the percent correct classification, with an estimated sensitivity of 88.6% and specificity of 96.5%. Use of Neospora caninum antigen following the same protocol demonstrated no difference in ELISA interpretation. Comparison with an existing indirect immunofluorescent antibody (IFA) test showed the ELISA to be the more sensitive and specific test for serodiagnosis of Neospora infection in cattle.
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Boot, R., H. C. W. Thuis, J. L. Veenema, and R. G. H. Bakker. "An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Pasteurella pneumotropica antibodies." Laboratory Animals 29, no. 3 (July 1, 1995): 307–13. http://dx.doi.org/10.1258/002367795781088306.
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An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats} mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity. The ELISA using P. pneumotropica NCTC 8284 detected more infected animals than selective culture in groups of rodents from which P. pneumotropica, Haemophilus sp and/or Actinobacillus sp were cultured. Cross reactivity between P. pneumotropica NCTC 8284 and haemophilus and actinobacillus isolates were not studied.
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NIU, QINGLI, ZHIJIE LIU, JIFEI YANG, PEIFA YU, YUPING PAN, BINTAO ZHAI, JIANXUN LUO, GUIQUAN GUAN, and HONG YIN. "Expression of sheep pathogen Babesia sp. Xinjiang rhoptry-associated protein 1 and evaluation of its diagnostic potential by enzyme-linked immunosorbent assay." Parasitology 143, no. 14 (October 17, 2016): 1990–99. http://dx.doi.org/10.1017/s0031182016001293.
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SUMMARYOvine babesiosis is one of the most important tick-borne haemoparasitic diseases of small ruminants. The ovine parasite Babesia sp. Xinjiang is widespread in China. In this study, recombinant full-length XJrRAP-1aα2 (rhoptry-associated protein 1aα2) and C-terminal XJrRAP-1aα2 CT of Babesia sp. Xinjiang were expressed and used to evaluate their diagnostic potential for Babesia sp. Xinjiang infections by indirect enzyme-linked immunosorbent assay (ELISA). Purified XJrRAP-1aα2 was tested for reactivity with sera from animals experimentally infected with Babesia sp. Xinjiang and other haemoparasites using Western blotting and ELISA. The results showed no cross-reactivities between XJrRAP-1aα2 CT and sera from animals infected by other pathogens. High level of antibodies against RAP-1a usually lasted 10 weeks post-infection (wpi). A total of 3690 serum samples from small ruminants in 23 provinces located in 59 different regions of China were tested by ELISA. The results indicated that the average positive rate was 30·43%, and the infections were found in all of the investigated provinces. This is the first report on the expression and potential use of a recombinant XJrRAP-1aα2 CT antigen for the development of serological assays for the diagnosis of ovine babesiosis, caused by Babesia sp. Xinjiang.
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Quispe Pari, Elizabeth. "Diagnóstico de teniasis humana mediante elisa coproantígeno y microscopía tradicional en poblaciones rurales de Puno - Perú." Revista Investigaciones Altoandinas - Journal of High Andean Investigation 17, no. 3 (December 30, 2015): 477. http://dx.doi.org/10.18271/ria.2015.152.
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<p align="center"><strong>RESUMEN</strong></p><p class="Default">La teniasis, es una enfermedad parasitaria endémica distribuida a nivel mundial, la detección de antígenos por coproantígeno tiene mejor sensibilidad diagnóstica. Los objetivos del estudio fueron: Determinar la prevalencia de <em>Taenia sp</em> en dos poblaciones rurales utilizando la técnica de microscopia y elisa-coproantigeno; Comparar la sensibilidad de elisa-coproantigeno con el análisis microscópico. Se analizaron 723 muestras de heces. Los resultados en: Copamaya por microscopia 1,7% (3/173) de positivos, mediante elisa-coproantigeno 2,8% (5/173). En Pharata por microscopia 2,2% (12/550), por elisa-coproantigeno 3,3% (18/550). En ambas localidades las edades de 30-59 obtuvieron mayor prevalencia. La prueba de elisa- coproantigeno detectó mayor número de casos en comparación con la microscopia. 12 muestras positivas por elisa-coproantigeno y microscopia se confirmó al observar el parasito en el tratamiento. En 7 muestras elisa-coproantigeno positivo y microscopia negativo no se pudo confirmar la presencia de taenia por que no se administró tratamiento por el bajo valor de porcentaje de positividad entre 16,28 a 31,28. Conclusión: La prevalencia de <em>Taenia sp</em> en Copamaya por microscopia es 1,7%; en Pharata 2,2%; por elisa-coproantigeno 2,8% y 3,3% respectivamente. La prueba de elisa-coproantigeno detectó mayor número de casos positivos frente al análisis microscópico, pero en algunos casos son complementarios para el diagnóstico de la teniasis. Mediante la prueba de t student para analizar las diferencias significativas de los dos métodos de diagnóstico se obtuvo (P=0.665). </p><p> </p><p align="center"><strong>DIAGNOSIS OF HUMAN TAENIASIS BY COPROANTIGEN ELISA AND TRADITIONAL MICROSCOPY</strong> <strong>IN THE RURAL POPULATIONS OF PUNO -PERU</strong></p><p align="center"><strong>ABSTRACT</strong></p><p>The tapeworm is a parasitic disease endemic in developing worldwide distributed, the antigen detection by coproantigen-Elisa has better diagnostic sensitivity. The objectives studies were: To determine the prevalence of <em>Taenia sp</em> in two rural populations using the technique of microscopy and coproantigen- Elisa; to compare the sensitivity of coproantigen-elisa with microscopic analysis. 723 stool samples were analyzed. The results show: In Copamaya it was possitive by microscopy 1, 7% (3/173), by coproantigen-elisa 2, 8% (5/173), while in Pharata by microscopy 2, 2% (12/550) and 3.3% (18/550) by coproantigen-elisa. In both locations the ages of 30- 59 had the greatest number of positive. Elisa-coproantigen test detected more cases compared with microscopy. 12 positive samples were confirmed by microscopy and coproantigen it is confirmed by the parasite in the treatment. In 7 samples coproantigen-elisa positive and negative microscopy could not confirm the presence of taenia because treatment is not administered by the low value of percentage of positivity between 16, 28 to 31,28. Conclusions: the prevalence of <em>Taenia sp</em> in population the Copamaya by microscopy is 1.7% and Pharata 2.2%, by coproantigen-elisa 2.8% and 3.3% respectively. The test coproantigen-elisa detected highest number of positive cases compared to microscopic analysis, but in some cases is complementary to diagnose tapeworm. Using the student t test to analyze the significant differences in the two diagnostic methods was obtained (P = 0.665).</p>
Dissertations / Theses on the topic "SP-ELISA"
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Maciel, Marilene Oliveira dos Santos. "ELISA plasmônica na detecção de anticorpos IgG anti-leishmania sp. /." Araçatuba, 2019. http://hdl.handle.net/11449/182290.
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Orientador: Valéria Marçal Felix LimaResumo: O cão tem sido alvo do controle da Leishmaniose Visceral (LV), pois são reservatórios potenciais de Leishmania infantum e desempenham um papel fundamental na cadeia epidemiológica da doença no homem. Portanto, o diagnóstico da leishmaniose canina (Lcan) no Brasil tem sido um desafio para os órgãos de controle de endemias, uma vez que apresentam limitações quanto à sensibilidade e especificidade em áreas endêmicas. Nesta perspectiva a presente pesquisa objetivou desenvolver e validar um ELISA plasmônica indireto rK28 (pELISA) para o diagnóstica da Lcan. Para o desenvolvimento do pELISA, foram realizados diferentes ensaios de otimização, determinação das concentrações ideais de peróxido de hidrogênio, íons ouro, anticorpo IgG anti-dog biotinilado e também do soro. Para a validação do ensaio, 170 amostras de soro de cães de área endêmica para Lcan e 26 amostras de cães saudáveis de área não endêmica para a doença foram testadas pelo pELISA e comparadas com ELISA indireto rk28 e com o teste imunocromatográfico (Dual Path Platform, TR_DPP®) usando como teste padrão-ouro o qPCR em amostras de sangue e/ou swab de subconjuntival. O ensaio foi padronizado com as concentrações de 250 μM de peróxido de hidrogênio, 0,30 mM de íons ouro e a melhor diluição do conjugado de estreptavidina-catalase foi de 1/50. O TR_DPP®, ELISA indireto rK28 e pELISA apresentaram sensibilidade de 79,0%, 89,5% e 94,7% e especificidade de 90,1%, 91,4% e 100,0%, respectivamente. Os maiores valores preditivos po... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Dogs have been the target of control of Visceral Leishmaniasis (VL) in humans, as they are potential reservoirs of Leishmania infantum and play a key role in the epidemiological chain of the disease. Therefore, the diagnosis of Canine Leishmaniasis (CanL) in Brazil has been a challenge for endemic control organs, since they have limitations on sensitivity and specificity in endemic areas. In this perspective the present research aimed to develop and validate an indirect plasmonic ELISA rK28 (pELISA) for the diagnosis of CanL. For the development of pELISA, different concentrations of hydrogen peroxide, gold ions, biotinylated anti-dog IgG antibody and serum were tested in order to establish ideal values to each parameter. For the validation of the assay, 170 dog serum samples from endemic area to CanL and 26 healthy dog samples from an area nonendemic to the disease were tested by pELISA and compared with indirect ELISA rk28 and the imunocromatografic test (Dual Path Platform, TR_DPP®) using as gold standard assay the real-time PCR in blood samples and/or subconjunctival swab. The assay was standardized with the concentrations of 250 μM hydrogen peroxide, 0.30 mM gold ions, and dilution of the streptavidin-catalase conjugate of 1/50. The TR_DPP®, indirect ELISA rK28 and pELISA presented sensitivity of 79.0%, 89.5% and 94.7% and specificity of 90.1%, 91.4% and 100%, respectively. The highest predictive positive (100%), negative (99.3%) and accuracy (99.4%) values were observed... (Complete abstract click electronic access below)
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Casimiro, Angélica Maria. "Padronização e avaliação de método sorológico ELISA para detecção de anticorpos IgG anti-Cryptosporidium sp." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-06032015-145009/.
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O presente trabalho teve como objetivo padronizar a técnica de ELISA para detecção de anticorpos IgG anti-Cryptosporidium sp para aplicação em estudos epidemiológicos da criptosporidiose em imunocompetentes. Para obtenção de antígeno, bezerros foram oralmente infectados. Os oocistos foram recuperados das fezes doanimal, com a utilização do gradiente de sacarose modificado, técnica de concentração onde se obteve o melhor rendimento. Para preparação do antígeno, os oocistos foram rompidos através de ciclos de congelamento/descongelamento e ultra-som. Soros controle positivo foram escolhidos entre o grupo de funcionários do laboratório de Parasitologia, pois apresentavam anticorpos anti-Cryptosporidium e devido as suas atividades no laboratório era um grupo mais exposto; soros controle negativo foram escolhidos entre aqueles com leituras de densidade óptica menores que 0,300 no ELISA para detecção de anticorpos anti-Cryptosporidium. Diferentes grupos de soros de indivíduos clinicamente normais (funcionários da parasitologia, doadores de sangue, pacientes que fizeram o Pré-Natal) ou outras infecções parasitárias (cisticercose, toxoplasmose, esquistossomose, Doença de Chagas, leishmaniose), foram avaliados para presença de anticorpos anti-Cryptosporidium. A alta freqüência foi observada para o grupo de pacientes com Doença de Chagas (66,6%) e baixa freqüência para o grupo de pacientes com esquistossomose e toxoplasmose (20,0%). A especificidade do teste ELISA para Cryptosporidium foi demonstrada com significante redução nas leituras de 0.0. observada em alguns soros após absorção dos anticorpos anti-Cryptosporidium. Além disso, no grupo de pacientes Pré-Natal 14,6%, quando comparada a alta freqüência de anticorpos anti-Cryptosporidium 52,0%, indica provável ausência de reações cruzadas entre os dois antígenos. Enfim, os resultados obtidos sugerem que a técnica de ELISA pode ser uma importante metodologia para aplicação em estudos soroepidemiológicos da criptosporidiose.The aim of the present study was to standardize an immunoenzymatic assay, ELISA, for detection of IgG antibodies to Cryptosporidium sp for use in epidemiologic studies on cryptosporidiosis. For antigen preparation, oocysts were obtained from fecal samples of orally infected calves. A modified sucrose gradient, concentration technique was used for recovered and purification of oocysts, which were ruptured by using freezethaw cycles and ultra-sonication. Positive control sera were chosen among the Parasitology workers, who presented anti-Cryptosporidium antibodies and had been exposed to this parasite, because of their activities in the laboratory; and negative control sera were chosen among the ones with optical density (O.D.) readings lower than 0,300 at ELISA for anti- Cryptosporidium antibodies. Oifferent groups of sera from clinically normal individuais (parasitology workers, blood donors, pregnant patients) or with other parasite infection (cysticercisis, toxoplasmosis, schistosomiasis, Chagas disease, leishmaniasis) were evaluated for the presence of Cryptosporidium antibodies. The higher frequency was observed for the group of patients with Chagas disease (66.6%) and the lower frequency for the group patients with schistosomiasis and toxoplasmosis (20.0%). The specificity of the Cryptosporidium-ELISA test was demonstrated when significant reduction of the 0.0. readings was observed for some serum samples after absorption of the anti-Cryptosporidium antibodies. Also, in the group of pregnant patients, the high frequency of 52.0% for anti-Cryptosporidium antibodies when compared to the low frequency of 14.6% for anti-Toxoplasma antibodies might suggest possible absence of cross reactions between these two closely related parasite antigens. The ELISA for detection of anti-Cryptosporidium antibodies, as standardized in the present work, can constitute a good toel for epidemiological studies of cryptosporidiosis.
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Santiago, Maria Emília Bodini [UNESP]. "Investigação de Leishmania spp. em Didelphis spp. (Linnaeus, 1756) na cidade de Bauru-São Paulo." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/94690.
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No período de março de 2005 a fevereiro de 2006, coletaram-se amostras de sangue e medula óssea de 112 gambás (Didelphis sp.) na região urbana da cidade de Bauru,estado de São Paulo, Brasil, com o objetivo de se verificar a possibilidade destes animais atuarem como reservatórios de Leishmania. Em amostras de soro foram detectados, anticorpos anti – Leishmania sp. pela técnica de ensaio imunoenzimático em fase sólida indireto (ELISA) e em medula óssea o DNA de Leishmania sp foi amplificado por meio da reação em cadeia pela polimerase (PCR) utilizando os primers 13A e 13B. Na reação de ELISA das 107 amostras analisadas 71,02% apresentaram se positivas, no PCR das 112 amostras de medula óssea analisadas 91,56% foram positivas. A evidência de fatores epidemiológicos de risco como, a presença do parasito circulante e dos vetores levam a acreditar que o gambá possa estar incluindo no ciclo da transmissão da Leishmaniose na cidade de Bauru.
Between March 2005 and February 2006, blood and Bone marrow samples were collected from 112 Opossums (Didelphis sp.) in the urban region of Bauru city - São Paulo state - Brazil . The objective was to verify the possibilities of these animals to be the reservoir of Leishmaniasis. Anti- Leishmania sp. antibodies were detected in serum by the Enzyme-Linked Immunosorbent Assay (ELISA) and DNA from Leishmania sp was detected in bone marrow through polymerase chain reaction (PCR). The 13A and 13B primers were used to perform the PCR. From 107 samples analysed with ELISA, 71,02% were positive for Leishmania sp, and positive outcome of 91,56% was observed by PCR. The evidence of epidemiologic risk factors like, the presence of a circulating parasite and of vectors leads to the conclusion that Opossums might be included in the leishmaniasis transmission cycle in Bauru city.
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Sakauchi, Dirce. "Desenvolvimento de um ensaio do tipo ELISA indireto utilizando anti-soro policlonal produzido em coelho contra a proteína SP-A suína para quantificar SP-A no lavado broncoalveolar humano." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-06012009-152702/.
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As colectinas pulmonares SP-A e SP-D são marcadores específicos de doenças pulmonares. A determinação destas proteínas por ensaios imunoenzimáticos permite aos clínicos correlacionarem seu papel funcional durante o desenvolvimento do processo patológico baseado nas anormalidades de suas concentrações. Como o lavado broncoalveolar (BAL) é uma amostra que permite estudar as proteínas secretadas pelo epitélio pulmonar em quaisquer circunstâncias, foi proposto o desenvolvimento de um ELISA indireto capaz de detectar SP-A no BAL humano usando um anti-soro policlonal contra SP-A suína produzido em coelho. SP-A suína foi purificada por protocolo que acopla precipitação ácida do extrato pulmonar suíno e cromatografia de afinidade. O anti-soro reagiu com as duas espécies de SP-A testadas: humana e suína. A curva padrão foi otimizada utilizando como calibrador a SP-A purificada de pacientes com artrite reumatóide. A faixa selecionada da curva de calibração foi de 0,312 to 5,0 mg/mL usando a diluição 1:1000 do anticorpo. O limite de detecção da curva foi de 0,625 mg/mL.The lung collectins SP-A and SP-D are specific markers for lung diseases. Determination of amounts of these proteins using polyclonal and monoclonal antibodies on ELISA assays enabled clinicians to predict their role in the course of the lung disease process based on abnormalities on their concentrations. As the bronchoalveolar lavage (BAL) is a sample that permits to study the proteins secreted by the lung epithelium at any conditions, we proposed to develop an indirect ELISA able to detect SP-A in the BAL using polyclonal rabbit antiserum, raised against porcine SP-A. Porcine SP-A was purified by a protocol that includes an acid precipitation of the porcine pulmonary extract before affinity chromatography. The antiserum reacted with the two tested species porcine and human. The calibration curve were optimized, using human SP-A purified from patients with rheumatoid arthritis as human antigen calibrator. The selected calibration curve range was 0.312 to 5.0 mg/mL using the antiboby dilution 1:1.000. The detection limit of the standard curve was 0.625 mg/mL.
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SIXDENIER, FABIENNE. "Detection de cryptospordium sp. Dans les selles de sideens : comparaison de trois methodes elisa avec la coloration de ziehl-neelsen modifiee et une technique d'immunofluorescence directe." Lyon 1, 1993. http://www.theses.fr/1993LYO1M341.
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Oliveira, Carlos Augusto Fernandes de. "Aflatoxina M1 em leite em pó distribuído pelo Programa de Alimentação Escolar do Município de São Paulo, SP-Brasil: utilização do ensaio por enzimas imuno-adsorvidas (ELISA)." Universidade de São Paulo, 1994. http://www.teses.usp.br/teses/disponiveis/6/6135/tde-24012018-111852/.
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Aflatoxina M1 (AFM1) foi pesquisada em 300 amostras de leite em pó distribuído pelo Programa de Alimentação Escolar do Município de São Paulo, SP-Brasil. As análises foram efetuadas através do ensaio por enzimas imuno-adsorvidas (ELISA), mediante o emprego de conjuntos de reativos produzidos em escala comercial. As amostras foram reconstituídas em água a 1:8 e submetidas diretamente ao ensaio, sem etapas de purificação. Os resultados revelaram 72 amostras (24,0 por cento ) positivas para AFM1, em concentrações de O,01 - 1,00 ng/ml, com média de O,15 ng/ml. Concentrações acima de 0,10 ng/ml foram observadas em 33 amostras (11,0por cento ). O método de ELISA foi avaliado no laboratório, através da execuçao de análises repetidas em amostras de leite experimentalmente contaminado com a toxina. Para os níveis de 0,10; 0,20; 0,50 e 1,00 ng/ml, os percentuais de recuperação foram, respectivamente, 83,0por cento ; 87,5por cento ; 103,0por cento e 111,8por cento . O desvio padrão relativo, para as referidas concentrações foi, respectivamente, 65,5por cento ; 31,8por cento ; 10,9por cento e 13,6por cento (n = 10, para cada nível de contaminação). Utilizando-se os dados de consumo de leite, adotados pelo Programa, para crianças de 4 meses de idade (máxima ingestão), estimou-se a ingestão diária média de 3,7 ng de AFM1/kg de peso corpóreo/dia. Discute-se a importância destes dados para a saúde humana, bem como os principais aspectos relativos ao estabelecimento de limites de tolerância para a AFM1 em leite e derivados.Aflatoxin M1 (AFM1) was surveyed in 300 samples of milk powder distributed by the School Food Supply Program of São Paulo, SP-Brazil. The analysis were performed by commercially available test sistems of competitive enzyme-linked immunosorbent assay (ELISA). Samples were reconstituted in water (1:8) and submitted directly to the assay, without clean-up procedures. Results showed 72 (24.0per cent ) positive samples for AFM1 at levels of 0.01 - 1.00 ng/ml (mean: 0.15 ng/ml). Concentrations above 0.10 ng/ml were observed in-33 samples (11.0per cent ). The method performance was evaluated experimentally in the laboratory, through repeated analysis of milk samples spiked with the toxin. Recoveries of AFM1 added to milk at levels of 0.10, 0.20, 0.50 and 1.00 ng/ml were 83.0per cent , 87.5per cent , 103.0per cent and 111.8per cent , respectively. Relative standard deviations for the concentrations refered were 65.5per cent , 31.8per cent , 10.9per cent and 13.6per cent , respectively (n = 10, per spiking level). By using data on milk consumption patterns, adopted by the Program for children aging 4 months (highest intake), a mean daily intake of 3.7 ng of AFM1/kg body weight/day was estimated. The implications of these data on human health, as well as the approaches for the establishment of regulations for AFM1 in milk and milk products, are discussed.
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Rossi, Claudio Nazaretian [UNESP]. "Ocorrência de Leishmania sp. em gatos do município de Araçatuba - São Paulo - Brasil." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/89230.
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Apesar de alguns relatos da ocorrência de leishmaniose visceral em felinos, a literatura é escassa no que diz respeito à sua pesquisa em populações de gatos de áreas endêmicas para a doença. Desta forma, o presente estudo teve por objetivo pesquisar em uma área endêmica para leishmaniose visceral canina, a possibilidade de infecção em gatos, por meio de exame parasitológico direto e da pesquisa de anticorpos anti-Leishmania chagasi pelas técnicas de ensaio imunoenzimático indireto (ELISA) e reação de imunofluorescência indireta (RIFI). Para tanto, foram colhidas amostras de soro de 200 gatos, encaminhados ao Centro de Controle de Zoonoses do município de Araçatuba - São Paulo - Brasil, bem como realizadas punções biópsias aspirativas de linfonodo, medula óssea, baço e fígado, utilizados para a confecção de preparados citológicos para a pesquisa direta de formas amastigotas de Leishmania sp. A prevalência da doença nessa população de gatos foi de 6,5%. Dos 200 animais avaliados, oito (4,0%) apresentaram resultado parasitológico positivo, seis (3,0%) apresentaram títulos sorológicos acima do ponto de corte (0,332) pela técnica de ELlSA e um (0,5%) evidenciou título superior ao ponto de corte (1:40) pela RIFI, totalizando 13 gatos considerados positivos.
In spite of some reports of the occurrence of feline visceral leishmaniasis, the literature is scarce about its research on populations of cats in endemic areas for the disease. The present work aimed to study, in an endemic area for canine visceral leishmaniasis, the infection possibility in cats using the direct parasitological test, enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent antibody test (IFAT). For this purpose, a total of 200 cats directed to the Zoonosis Control Center of the municipal district of Araçatuba - São Paulo - Brazil were employed. Lymph node, bone marrow, spleen and liver aspiration biopsies were carried out and observed under optical microscope to search for amastigote forms of the parasite and serum samples were submitted to serological methods in order to detect anti-Leishmania chagasi circulating antibodies. The prevalence of the disease in this population of cats was 6.5%. Amastigote forms of the parasite were observed in eight (4.0%) cats; by ELISA method, six (3.0%) cats presented titer above the specie's cut off point (0,332) and one cat (0,5%) showed a titer above 1:40, a positive serological reaction, by IFAT, totalizing 13 positive cats.
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Triques, Nelise Juliane. "Detecção de anticorpos contra Salmonella sp. em suco de carnes de suínos." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2008. http://hdl.handle.net/10183/13949.
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Silva, Fabio Jorge Moreira da. "Rela??o entre infesta??o natural por Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) e n?veis de anticorpos da classe IgG para os agentes da Tristeza Parasit?ria Bovina e Borrelia sp. em bezerros." Universidade Federal Rural do Rio de Janeiro, 2008. https://tede.ufrrj.br/jspui/handle/tede/755.
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Previous issue date: 2008-02-28Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
This study was conducted with the objective to contribute with the agreement of the relation calves x ticks x hemoparasites in the sector of milk cows of the Farm of the Institute of Zootecnia (FAIZ) of the Universidade Federal Rural do Rio de Janeiro (UFRRJ). Seventeen female calves with age between 15 days old and 14 months old, between july of 2006 and june of 2007. These animals were subdivided in three ages bands: up to 2 months, between 3- 6 months and above of 7 months, in accordance with the handling of the property. Was realized ticks`s counting, collection of blood and hematological examination of all the animals in interval of 14 days. The exams were carrying through in laboratories of Parasites Diseases, Clinical Pathology of UFRRJ and the Serological of Embrapa Beef Cattle. Throughout 12 months, it can be verified the constant presence of larvaes, nymphs and females of tick Rhipicephalus (Boophilus) microplus. The frequency of positive animals for the indirect enzyme-linked immunosorbent assay (ELISA), for the agents of the Tick-borne Disease (Babesia bigemina, B. bovis and Anaplasma marginale) and Borrelia sp., was verified that in all ages bands exist positive serological animal. The frequency and antibody levels, as much for B. bigemina as for B. bovis, evaluated through the indirect ELISA, had been high. This fact associated with the absence of infection symptoms suggests a situation of immunization of the animals and an area of enzootically stable. None trend of seasonal distribution of infections for B. bigemina, B. bovis, A. marginale and Borrelia sp. was observed.
Este estudo foi conduzido com o objetivo de contribuir para o entendimento das rela??es bezerros x carrapatos x hemoparasitos no setor de bovinocultura de leite da Fazenda do Instituto de Zootecnia (FAIZ) da Universidade Federal Rural do Rio de Janeiro (UFRRJ). Foram utilizadas 17 bezerras com idade entre 15 dias e 14 meses, entre julho de 2006 a junho de 2007. Estes animais foram subdivididos em tr?s faixas et?rias: at? 2 meses, de 3-6 meses e acima de 7 meses, de acordo com o manejo zoot?cnico da propriedade. Procedeu-se contagem de carrapatos, coleta de sangue e exames hematol?gicos de todos animais em intervalo de 14 dias. Os exames foram realizados nos Laborat?rios de Doen?as Parasit?rias, de Patologia Cl?nica da UFRRJ e de Sorologia da Embrapa Gado de Corte. Ao longo de 12 meses, podese verificar a presen?a constante de larvas, ninfas e f?meas de carrapatos Rhipicephalus (Boophilus) microplus. Em rela??o a freq??ncia de positividade pelo ensaio de imunoadsors?o enzim?tica (ELISA) indireto, para os agentes da Tristeza Parasit?ria Bovina (Babesia bigemina, B. bovis e Anaplasma marginale) e Borrelia sp., verificou-se que em todas as faixas et?rias haviam animais sorologicamente positivos. A freq??ncia e os n?veis de anticorpos, tanto para B. bigemina como para B. bovis, avaliados atrav?s do ELISA indireto, foram altos. Este fato associado ? aus?ncia de sintomas de infec??o sugere uma situa??o de pr?-imuniza??o dos animais e uma ?rea de estabilidade enzo?tica. N?o foi observada qualquer tend?ncia de distribui??o sazonal de infec??es por B. bigemina, B. bovis, Anaplasma marginale e Borrelia sp.
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Cordeiro, Daniela. "Uso de bioindicador de efeito endócrino e validação do método para determinação de hormônios na água da Represa Municipal de São José do Rio Preto, SP." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-11032010-102102/.
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Dentre os vários xenobióticos que as atividades humanas têm produzido nas últimas décadas, os desreguladores endócrinos (EDs), incluindo os hormônios, vêm chamando a atenção de pesquisadores devido aos efeitos que eles causam em animais. Esses efeitos podem resultar em características hermafroditas nos peixes e em anfíbios, inibição do crescimento testicular, inibição da espermatogênese, decrescimento da capacidade de fertilização dos ovos e alteração no comportamento reprodutivo dos seres vivos. Concentrações de apenas 10 ng L-1 de hormônio no meio aquático já são capazes de causar efeito endócrino nos organismos. Neste estudo determinou-se o hormônio natural 17β-estradiol e os hormônios sintéticos levonorgestrel e 17α-etinilestradiol na água da Represa Municipal de São José do Rio Preto (SP). A primeira etapa deste estudo foi a validação dos métodos segundo a Resolução-RE 899 da ANVISA. Os limites de detecção, de quantificação e inferior de quantificação do método para a determinação do 17α-etinilestradiol foram, respectivamente, 25, 100 e 100 ng L-1. A linearidade, desvio-padrão relativo, exatidão e recuperação média para o 17α-etinilestradiol foram, respectivamente, R de 0,98, 3,23%, 100,53% e 89,95%. Os limites de detecção, de quantificação e inferior de quantificação do método para a determinação do 17β-estradiol foram, respectivamente, de 100, 150 e 150 ng L-1. A linearidade, desvio-padrão relativo, exatidão e recuperação média do 17β-estradiol foram, respectivamente, R de 0,99, 3,43%, 106,16% e 89,05%. Para o levonorgestrel, os limites de detecção, de quantificação e inferior de quantificação foram, respectivamente, 50, 150 e 150 ng L-1. A linearidade, desvio-padrão relativo, exatidão e recuperação média do método para a determinação do levonorgestrel foi respectivamente, R de 0,98, 3,48%, 105,15% e 86,45%. Na segunda etapa desta pesquisa analisaram-se amostras de água coletadas na Represa Municipal de São José do Rio Preto (SP) quanto à presença de hormônios. Como método de extração dos hormônios, usou-se a SPE e, como técnica analítica HPLC/FLU/DAD. Os resultados não indicaram a presença dos hormônios estudados até o limite de detecção do método empregado. Foi feita também a análise de vitelogenina (VTG) em plasma sanguíneo de peixes das espécies Geophagus brasiliensis (cará), Satanoperca pappaterra (zoiúdo) e Tilapia rendalli (tilápia rendali) capturados na referida represa. Observou-se que os peixes machos continham concentração de VTG na faixa de 152,4 a 2.841,8 ng mL-1. Isto indica que há substâncias de efeito endócrino na água da represa, mas não se pode afirmar que sejam os hormônios estudados.Among the several xenobiotics that human activities have produced in the last decades, endocrine disruptors (EDs), including hormones, have been drawing the attention of researches due to the effects they can cause in animals. Those effects may result in hermaphrodite characteristics in fishes and amphibians, testicular growth inhibition, spermatogenesis inhibition, eggs fertilization capacity decrease, and changes in the reproductive behavior of living beings. Concentrations of only 10 ng L-1 of hormones in the aquatic medium are capable of causing endocrine effects in organisms. In this study, the natural hormone 17β-estradiol and the synthetic ones levonorgestrel and 17α-ethinylestradiol were determined in the waters of the São José do Rio Preto (SP) dam. The first step of this study was the validation of the methods according to ANVISA\'s Resolution 899. The detection, quantification, and lower quantification limits of the method for determining 17α-ethinylestradiol were, respectively, 25, 100, and 100 ng L-1. The linearity, relative standard deviation, accuracy, and average recovery of the method for determining 17α-ethinylestradiol were, respectively, R equal to 0.98, 3.23%, 100.53%, and 89.95%. The detection, quantification, and lower quantification limits of the method for determining 17β-estradiol were, respectively, 100, 150, and 150 ng L-1. The linearity, relative standard deviation, accuracy, and average recovery of the method for determining 17β-estradiol were, respectively, R equal to 0.99, 3.43%, 106.16%, and 89.05%. For levonorgestrel, the detection, quantification, and lower quantification limits of the method were, respectively, 50, 150, and 150 ng L-1. The linearity, relative standard deviation, accuracy, and average recovery of the method for determining levonorgestrel were, respectively, R equal to 0.98, 3.48%, 105.15%, and 86.45%. In the second step of this research, samples collected in the São José do Rio Preto (SP) dam were analyzed regarding the presence of hormones. For extracting the hormones, SPE cartridges were used followed by HPLC/FLU/DAD. The results indicated the absence of the studied hormones down to the detection limits of the methods employed. Vitellogenin (VTG) analyses were performed in the blood plasma of fishes captured in the beforementioned dam, of the species Geophagus brasiliensis (pearl cichlid or pearl eartheater), Satanoperca pappaterra (Pantanal eartheater or Paraguay River eartheater), and Tilapia rendalli (redbreast tilapia). It was observed that male fishes had VTG concentrations between 152.4 and 2,841.8 ng mL-1. That indicates that there are substances with endocrine effect in the dam water, although one cannot say the studied hormones are among them.
Book chapters on the topic "SP-ELISA"
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Dilger, I., G. Schwedler, S. Janßen, and J. W. Dudenhausen. "Messung der hydrophoben Surfactant-Proteine SP-B und SP-C im Fruchtwasser mit einem Kompetitions-ELISA als Beitrag zur antenatalen Lungenreifediagnostik." In Gynäkologie und Geburtshilfe 1992, 1451–52. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77857-5_581.
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